eChapters

Abstract

Agriculture is the major sector for the livelihood of the billions of people worldwide. Increasing population and decreasing arable lands are the major concern for the fulfillment of the desired food production. After green revolution more production in the crop is obtained by worldwide. Several insects and pests are responsible for the inhibition of desired production in agriculture. Therefore, pesticides play a major role in the control of destructive insects and pests. After ban of organochlorine pesticides, organophosphorus pesticides has been used vigorously. Beside the effective pest management in agriculture, residual organophosphorus pesticides are more lethal for human and other living creature. Monitoring of these compounds is very much essential in the food and agricultural samples. Chromatographic techniques are well adopted techniques for the hundreds of residual pesticides. Introduction of various analyzers in chromatographic techniques improve the monitoring of the residual pesticides from different samples. Therefore, recent advancement in residual pesticides isolation and chromatographic techniques has been discussed in this book chapter.

Drying of the Spices

Drying is an important mode of preservation and it ensures microbial safety of the spices.The main purpose of drying is to increase the shelf storage and make the seasonal spices available round the year with minute losses of nutrients, flavor, taste and color (Maroulis, 2003). During drying, there is a continuous transfer of heat and mass due to the heat application which eventually leads to moisture removal from the product (Akpinar, 2006) (Hashim, 2014). Since, the sensory, nutritional and functional attributes of spices are partially or totally affected by the drying process, the adequate choice of drying technique becomes requisite. There exists a number of conventional drying methods including solar drying (El-sebaii, 2012), freeze drying (Ciurzy nska 2011), vacuum drying (Nadi, 2012), osmotic dehydration,hot air drying and fluidized bed drying (Onwude, 2016). However, these drying techniques lead to poor quality of the spices because of the longer duration of treatment and high amount of energy. To combat with the issues of poor quality, there has been a significant development of the novel techniques, which includes- microwave, ohmic, infrared, pulse electric field, radio- frequency, ultraviolet, ultrasound, supercritical and heat pump heating, in the drying of the agricultural crops. These novel techniques are found to produce higher quality products as compared to the conventional drying techniques (Moses J. N., 2014). The microwave and radio frequency heating technologies are developed in such a manner that they are capable of partial or complete removal of the conventional methods of heating/ drying (Onwude, 2016). The use of these novel techniques has gained a keen attention for the drying purpose of spices. Some of the researchers have also proved that combination of conventional and novel drying techniques leads to better product output.

1. Introduction
Medicinal organic chemistry is a branch of chemistry focused on the design, development, and synthesis of organic molecules that can be used as therapeutic agents. It connects biology, pharmacology, and organic chemistry and is essential to the process of finding and developing new drugs. Here are some foundational concepts in medicinal organic chemistry:
1.1. Drug Discovery and Design
Medicinal chemists aim to identify bioactive compounds that can interact with specific biological targets, such as proteins or enzymes, to produce a desired therapeutic effect. The discovery process often starts with lead compounds, which may be found in natural sources (e.g., plants, fungi) or through high-throughput screening of large chemical libraries. Drug discovery and design (Zhong et al., 2014; Patani et al., 1996; Lipinski C. A., 2004) are critical aspects of medicinal organic chemistry, focused on identifying and optimizing chemical compounds with potential therapeutic effects. This complex, multi-step process combines organic chemistry, biology, and pharmacology to create drugs that are both effective and safe for clinical use (Figure 1). Below is a summary of the primary phases: Fig. 1: Drug discovery and development stages from target identification to clinical trials
1.1.1. Target Identification and Validation
The first step in drug discovery is identifying a biological target (Schenone et al., 2013), often a protein, enzyme, or receptor associated with a specific disease. This target must be validated, meaning that it is confirmed as essential for the progression of the disease. For instance, the identification of enzymes that play a role in cancer cell growth can provide targets for anticancer drug development. The list of some targets and their therapeutic application are given in table 1.
1.1.2. Hit Identification and Lead Compound Discovery
After identifying a target, medicinal chemists seek “hit” compounds that show initial biological activity against it. High-throughput screening (HTS) methods are often used to test thousands of compounds from chemical libraries to find potential hits. These hits are then refined to identify “lead” compounds, which exhibit stronger or more specific activity.

Two cornerstone methodologies in genetics, forward genetics and reverse genetics, illuminate the intricate connection between gene sequences and their functions. Forward genetics traditionally entailed laborious steps, beginning with the observation of mutants and culminating in the identification of causal genetic alterations. While effective, this approach was time-consuming and limited in scalability. Recent innovations have revolutionized forward genetics with next-generation sequencing (NGS), genome-wide association studies (GWAS), CRISPR (clustered regularly interspaced short palindromic repeats)-based screens, and advanced computational techniques. These advancements deliver precision and efficiency, enabling researchers to explore gene function on an unprecedented scale. In contrast, reverse genetics initiates its journey armed with sequence data and harnesses the manipulation of genes to observe resultant phenotypic shifts. Traditional reverse genetics, though invaluable, faced challenges like limited precision and scalability. Cutting-edge approaches have transformed the landscape. Techniques such as prime editing, base editing, high-throughput functional genomics, single-cell genomics, and synthetic biology offer unparalleled precision and capacity. They empower scientists to scrutinize gene function at the single-cell level, conducting precise genome editing and large-scale functional genomics studies. These strides in forward and reverse genetics have charted new territories for genetic research.

Introduction
Fisheries and aquaculture are crucial for global food security, providing a significant portion of the world’s animal protein supply. With the rapid growth of the human population and the overfishing of natural fish stocks, it is more important than ever to increase aquaculture production sustainably. Advances in molecular cytogenetics are crucial for promoting sustainable growth and innovation in fisheries and aquaculture. These advancements offer deeper insights into the genetic factors that govern key traits like growth, reproduction, and environmental resilience, enabling aquaculture to address global food demands while safeguarding biodiversity and maintaining genetic
integrity. The integration of these techniques in fisheries and aquaculture has led to significant advancements in the genetic improvement of cultured species, disease management, and biodiversity conservation in recent years. Moreover, these scientific advancements offer innovative methods for effective disease control and the preservation of aquatic biodiversity, all of which contribute to aquaculture’s long-term viability and growth. Over the past two decades, fish karyotype studies have been combined with genome sequencing, revealing more remarkable genome plasticity and variability in fish compared to other vertebrates. Comparative fish genome analysis evaluates divergence rates and explores sex-determination genes and gene family evolution in teleosts. ,Currently, over 80 fish species have their genomes annotated in the Ensembl database (https: //www.ensembl.org/index.html, accessed on 6 April 2021), and around 150 are assembled to the chromosome level in the NCBI database (https://www.ncbi.nlm.nih.gov/genome/). However, the quality of many assembled genomes has been criticized for not meeting reference genome standards. Fish genome sequencing is expected to grow significantly by 2028 due to initiatives such as the Earth BioGenome Project. This chapter will explore the chronological development of cytogenetic techniques used in aquaculture and fisheries, emphasizing their role in improving quality, ensuring disease resistance, and promoting environmentally sustainable practices.

Field-ready pregnancy diagnostic tests are an important tool for improving reproductive performance in livestock. They help optimize breeding programs, resource allocation, and decision-making processes, leading to better economic outcomes and sustainable livestock production systems. Pregnancy diagnosis is an important tool for improving reproductive performance in livestock for several reasons:

1) Timely management: early and accurate pregnancy diagnosis allows farmers and livestock producers to make timely decisions about the management of pregnant and non-pregnant animals. This includes adjusting feeding regimes, providing appropriate veterinary care, and establishing breeding plans.

2) Optimized breeding programs: In livestock, controlling the timing of pregnancies is critical to maintaining a well-organized breeding program. With accurate pregnancy testing, farmers can identify non-pregnant animals and breed them back earlier, optimizing resource utilization and shortening the generation interval.

3) Resource allocation: early identification of non-pregnant animals helps avoid unnecessary allocation of resources such as feed and health care to animals that will not produce offspring in the current cycle. This efficient allocation of resources is essential to maintaining a cost effective production system.

4) Lower costs: unproductive animals consume resources without contributing to farm productivity. By quickly identifying non-pregnant animals, farmers can avoid the cost of keeping these animals during their non-productive period.

5) Genetic improvement: for livestock programs focused on genetic improvement, accurate pregnancy diagnosis is critical. It enables the selection and promotion of animals with desirable traits for breeding purposes, thus accelerating genetic progress.

INTRODUCTION

The aquaculture sector expands and environmental regulations become stricter, waste management has emerged as a significant responsibility for managers of aquaculture farms. Waste generated by aquaculture facilities usually comes in the form of either effluent or sludge. Effective management and disposal of wastewater in aquaculture are becoming progressively crucial due to the strict regulations concerning the discharge of waste into natural water systems. The worldwide expansion of aquaculture industries has led to a rise in adverse environmental effects due to the release of significant quantities of polluting effluents containing uneaten feed and fecal matter (Chávez Crooker and Obreque-Contreras, 2010) Efficient management of wastewater in aquaculture is crucial for fostering sustainable practices. A variety of innovative methods have been developed to treat aquaculture wastewater effectively. These encompass eco-sensitive systems such as aquaponics and integrated multi-trophic systems, along with biofilm technologies that exploit the mutual growth of algae and fungi to remove nutrients. Furthermore, the reuse of wastewater via oxidation ponds can both provide natural sustenance for fish and treat household sewage effectively. Technologies like constructed wetlands and recirculation systems have proven highly effective in removing nutrients and recycling water, thus aiding in sustainable aquaculture practices. Additionally, specialized continuous automatic treatment equipment has been devised specifically for aquaculture wastewater, ensuring efficient purification processes. Together, these diverse strategies collectively strive to address the challenges posed by nutrient pollution and resource depletion in managing aquaculture wastewater.

Introduction

Ber belongs to the family Rhamnaceae, which consists of about 45 genera and 950 species. Out of 950, the genus Ziziphus consists of about 80 species of evergreen and deciduous climbers, shrubs, and trees found in the old world tropics, subtropics, and warmer temperate regions. The fruits of almost all species are edible, and jujube (Z. jujuba Mill.) and ber (Z. mauritiana Lamk.) are cultivated commercially. Liu and Cheng (1995) believed that the Indo Malaysia region (South and South East Asia) is the center of both the evolution and distribution of the genus Ziziphus.

Ber is the most common fruit of arid and semi-arid parts of the world due to its hardy nature and wild adaptability to soil and climatic conditions. It survives and produces economically viable yield in the hyper-arid zone where most of the trees fail to grow due to lack of moisture, extreme temperature, and poor soil conditions. It performs well under saline and alkaline soil conditions. It can produce economically viable yield with moderately saline irrigation water. Ber can withstand extreme heat but is susceptible to frost. Furthermore, due to its deep root system and other xerophytic characteristics, it is extremely drought tolerant but requires adequate soil moisture during fruit development. While high atmospheric humidity is unsuitable for ber cultivation, low humidity during fruit development improves fruit quality.

Litchi (Litchi chinensis Sonn.), a subtropical perennial fruit tree, belongs to family Sapindaceae and is indigenous to China. Its cultivation spread to the neighbouring countries of the south-east Asian region and other countries. The fruit of litchi is small, heart-shaped, conical or spherical in shape and pinkish red to bright-red in colour, with varying colour intensity in different cultivars. The pulp of fruit is crispy and grape-like in texture, very succulent and aromatic and is generally characterized by a sweet, acid, juicy and soft taste. Because of these reasons, litchi is highly prized in its fresh form as well as in different processed formulations. The total litchi production is about 24,00,000 tonnes from the net cultivated are of about 8,00,000 ha. The main litchi-producing countries are China, India and Thailand, whereas it is commercially grown also in many other countries. Of the total world litchi production, China and India contribute about 90%. Although litchi plants are long-lived, even then new plantings of this fruit are being done regularly, ensuring the continuous growth of production at the global level. In India, where this fruit can be grown in off season also in the southern states of Karnataka, Tamil Nadu and Kerala, the litchi production is about 7,37,200 tonnes from an area of 99,170 ha (Chandrakala et al., 2023).
 

Introduction

• India is the second largest producer of fruits in the world after China

• India is rich in fruit diversity starting from tropical, subtropical to temperate region.

• Some of the fruits like Guava, pomegranate, lemon, mandarin etc., if left without any treatment, give several light harvests of the variable quantities and qualities from the various flowering flushes throughout the year.

• Some of the fruit crops bloom throughout the year without any resting period and produces two or three crops (bahar) in a year but yield and quality is not so good in all crop harvest. So, it is very essential to understand the flowering and fruiting behavior of crops and which bahar will give good crop with considering all the factors associated with a particular bahar.

7.1 What is crop regulation?

• Crop regulation means to force the tree for its rest and to produce blossom and fruits during any one of two or three flushes”

• Crops in which flowering is continuous throughout the year; economic yield cannot be achieved in any of the season.

• Therefore, by analyzing market demand flowering can be forced in particular one season, which gives the economic yield.

• Through various techniques like cultural practices and chemical treatments, trees are thrown to rest prior to flowering and then practices are done to trigger flowering.

• Thus, crop regulation is a method to regulate flowering by altering natural tendency of the tree by artificial means.

Reasons for serious concern for food security in developing countries are, increasing food demand for the rapidly growing population, decline in productivity in high productive areas due to ‘Green revolution’ fatigue, and increasing vulnerability of agriculture to potential climate change. Climate scientists predict that global warming will make arable land in many developing countries less productive or unusable. Of all the parameters, abiotic environmental stresses contribute significantly to reduction in potential yield (Flowers and Yeo, 1995). Abiotic stresses arise from extremes of climate. The stress factors especially drought and salinity negatively effect plant growth and development, salt stress being one of the most serious environmental factors limiting productivity of crop plants (Ashraf, 1999). Therefore, extensive research into plant salt tolerance was carried out, with the aim of improving resistance of crop plants. With a large majority of the world’s population relying on crops like barley, maize and rice to survive, crop salt tolerance is globally important.

Introduction

Farm mechanization is application of machine power to work on land, usually performed by animate and mechanical power. It is also defined as an economic application of engineering technology to increase the labour efficiency and productivity. However, it not only includes production, distribution and utilization of a variety of tools, machinery and equipment but also planting, harvesting and primary process. Mechanization has a major impact on demand and supply of farm labour, agricultural profitability and a change in rural dynamics.

Abstract

It is well known that drying is an important unit operation in food processing industries, essentially used to extend the shelf life but also in making innovative processed products such as snacks with desired functionalities. Although drying of food products is carried out traditionally using several methods, the quality and energy aspects have been major focus of food drying research in the last decade or two. This is achieved by modifying and/or optimizing the existing drying systems, improving pre and post processing of the product, combination of more than one dryer type or developing altogether a new drying technique. There several dryer types which can give extremely good product quality, however, cost-effectiveness can be a major issue. It is necessary to balance both good product quality and minimum energy usage in order to sustain highly competitive market. This lecture will provide an overview of conventional drying methods used for foods, followed by some advances in these conventional methods and a discussion on some new drying techniques developed in recent years that are either commercialized or have potential of industrial use.

The Indian food industry is poised for huge growth, increasing its contribution in world food trade every year. In India, food processing is gaining importance as now it is being considered as profit industry. The increased demand for processed food in the domestic market and Indian foods getting popularized in the global market has put food processing industry on high priority list by the entrepreneurs. Accounting for about 32 percent of the country’s total food market, the food processing industry is one of the largest industries in India and is ranked fifth in terms of production, consumption and export. The total food production in India is likely to double in the next ten years with the country’s domestic food market estimated to reach US$ 258 billion by 2015. The food industry in India has been attracting a lot of attention from foreign investors in the area of processing, development of infrastructure for storage and transportation and retail marketing and scenario is changing rapidly.

Introduction

Livestock is an important component of Indian agriculture contributing 28% of agricultural gross value added (GVA) of the country. Agriculture, as such, contributes 16.4% of country's GVA (Economic survey, 2017-18). Livestock as a source of income has seen a steady increase from 4% to 13% in a decade time (2002-2012). India, with 2.29% of the world land area, is maintaining about 10.71% of the world’s livestock population. The milk production in the country increased from 17 million tonnes in 1950-51 to 187.7 million tonnes in 2018-19. The feed and fodder play an essential role in the health and productivity of livestock. Even though India is the world largest milk producer with 21% of global production, the productivity (1700 kg/year) is far below the world average (2574 kg/year) (FAO STAT, 2018). One of the main responsible factors (50.2%) for this low productivity is the deficiency in feed and fodder. Although forage crops are the critical component of livestock-based farming system, the area under cultivated fodder is static to around 8.4 million ha (5.23%) since last two decades due to the competition of land use with other crops. Availability of the fodder determines the productivity and profitability of the livestock rearing because fodder and feeds constitute about 60% of the total cost of milk production, which can be lowered by enhancing the green fodder-based feeding system. The traditional grazing lands are gradually diminishing because of urbanization, expansion of the cultivable area, grazing pressure, industrialization etc. These factors coupled with stagnation of area under cultivated fodder resulted in a severe shortage of feed and fodder to the extent of 26% in dry crop residues, 35.6% in green fodder and 41% of concentrates (IGFRI, 2011). This gap in demand and supply may further rise due to consistent growth of livestock population at the rate of 1.23% in the coming years. The share of the in-milk population to total population has increased from 23% to 27% in the case of cattle and from 33.64 to 34.74% in buffaloes from 2012 to 2019. The year-on-year growth rate of milk is approximate 6%. Thus, to sustain this growth rate and for further expansion to meet the demands of the ever growing human population, livestock needs a sustainable supply of feed material. To reduce the demand and supply gap, the production and productivity of fodder crops need to be enhanced. The productivity of cultivated fodder crops is low, due to the least attention and allocation of minimal production resources and lack of dissemination of the production techniques to stakeholders involved in the forage resource development. The present chapter highlights the recent advances in the forage crop production technologies. The comprehensive compilation of information at one place provides the opportunity to get the holistic picture of forage production altogether.

Abstract

Fusion is a well known entity in various fields (e.g. medical imaging, weapon detection, flood monitoring etc.) that surmises information originating from several modalities. This chapter is an attempt to assort the various image fusion techniques available for human face recognition and to put a review of the advancements being made by the different researchers. The various types of fusion those have been used or may be used for face recognition have also been discussed here.

Nutrient use efficiency of crops is being improved through temporal and spatial management of the form and amount of nutrient inputs as being demonstrated by various agronomic practices. In addition, understanding of the genetic basis of nutrient efficiency would lead to the development of nutrient use efficient varieties thus reducing the application of nutrients. Earlier studies on genetics of nutrient use efficiency were limited as increasing productivity with heavy nutrient inputs was the main focus. But in the backdrop of environmental degradation due to the excessive nutrients and its impact in climate change, nutrient use efficiency in crops is need of the hour for sustainable and eco-friendly agriculture.

Introduction 
Fossils fuel resources are crucial for producing and distributing other goods, making them one of the most important global commodities. Fossil fuels account for more than 75% of total energy consumption in society. It is critical to recognize that the fossil energy problem and anthropogenic climate change problem are inextricably linked and must be addressed as two intertwined challenges requiring a comprehensive solution. The features of coal, oil and gas which are employed as fossil fuels for power generation, have different classification of characteristics (Shindell & Smith, 2019). Fossil fuels are natural fuels and derived from the decompositions, heating and pressurization, of buries phytoplankton (plants) and zooplankton (animals). Over time, these waste materials get decomposed to produce carbon rich deposits that are now used to create energy products (Holechek et al., 2022). They are essential for the synthesis of steel and polymers in addition to energy. Fossil fuels fall into three main categories: coal, oil, and natural gas (Singh et al., 2023). Biofuels are produced from plants and animals waste materials and considered as a renewable source of energy. The sources are key components to overcome the issues related to greenhouse gas emission. Due to rapid change in climatic conditions, demand of renewable energy sources has increased. There are some drawbacks for fossil fuels due to presence of high carbon footprints and environmental impacts. On the other way, biofuels offer two major benefits first they reduce carbon emissions and modify waste management method, the other paving the way for a sustainable future. The carbon neutrality of biofuels is among their most alluring features. Biofuels only release the carbon dioxide that plants consume during growth, as opposed to fossil fuels that release old, stored carbon into the atmosphere. They are an eco-friendly choice because of this balance(Jeswani et al., 2020). Bioethanol, biodiesel, and biogas are among the several types of biofuels(Esmaeili & Tamjidi, 2021). Bioethanol is used in transportation and produced by using crops like corn and sugarcane. Waste management and sustainable energy production are further enhanced by biogas after producing with the help of anaerobic digestion of organic waste and is a great source of energy for heating and power generation. We can take a comprehensive approach to sustainability, improving energy efficiency and lessening our environmental impact, by combining the production of biofuel with waste management and the use of organic fertilizers. Significance environmental benefits of biofuels include lower GHG emission, reduced dependency on the fossil fuels and better air quality. By diversifying energy sources and possibly stabilizing prices, they also lessen exposure to changes in the world's oil markets, which promotes energy security. Nonetheless, it is essential to evaluate the wider ramifications of biofuel production and make sure that its advantages exceed any potential disadvantages in order to completely match with sustainability goals(Jeswani et al., 2020).

Introduction
Crop breeding programmes are under increasing pressure for developing cultivars that possess the ability to adapt to diverse environments while maintaining higher quality and quantity, in response to the escalating worldwide demand for food. The primary goal of plant or crop breeding is to attain genetic enhancement, leading to the creation of new varieties that demonstrate higher yield and superior quality. Hence, the central aim of crop breeding initiatives is to develop novel cultivars that demonstrate improved resistance against fungal, bacterial, insect, or viral pathogens, alongside increased tolerance to abiotic stresses such as drought low temperature, salinity, dehydration, and heavy metal toxicity. Additionally these breeding efforts aim to improve quality attributes, including taste, size shape, colour, and cooking convenience (Lenaerts et al., 2019). The ability to anticipate emergent phenotypes resulting from genetic or environmental disruptions necessitates the utilization of methodologies capable of identifying metabolic pathways or canopy structures suitable for change. The insilico tools have the potential to guide breeding endeavours aimed at developing novel germplasm that exhibits robust adaptation to diverse environmental conditions. The precise simulation of organisms in a computational environment is a current and effective approach to enhance prediction abilities. Significant progress has been made in the field of predictive medicine through the implementation of community projects that utilize integrative, multi-scale modeling techniques. However, there are a lot of crop models that are just geographically and temporally restricted, which limits their ability to go beyond the data that is now available and, consequently, leads to insufficient forecasts regarding crop reactions in novel circumstances. The plant community has launched or continued a coordinated endeavour focused on the development of a virtual plant modelling through consolidative and multi-scale mapping, models and simulations (Marshall-Colon et al., 2017).

Climate-based/smart (digital) irrigation practices and its management, irrigation automation, sensors based irrigation, robotic application in irrigation and artificial intelligence
Climate-smart (digital) irrigation: Aims and Objectives
Climate-smart irrigation (CSI) is a vital part of climate-smart agriculture,
aiming to
• Boost the productivity of irrigated farming and farmer incomes while minimizing negative social, political, and environmental consequences.
• Bridge the gap between potential and actual crop yields across different climates.
• Enhance the resilience of irrigated farming and related supply chains to current and future climate change impacts and other risks.
• Adapt irrigated farming to climate change by capitalizing on new opportunities and mitigating adverse effects.
• Reduce greenhouse gas emissions per unit of food, fiber, or fuel produced in irrigated systems, from farm to market.
• Identify and prioritize ways to cut GHG emissions from irrigated systems at various stages, from water sourcing to post-harvest processes.
• Improve the environmental sustainability of irrigated farming and supply chains while safeguarding water access for communities, protecting vulnerable groups’ livelihoods, and preserving aquatic ecosystems.
• Improve the sustainability and reducing the GHG emissions of existing crop production systems.
Irrigation is key to stable agricultural production, especially in dry conditions. CSI aims to improve existing and new irrigation systems by incorporating climate-smart principles. While good irrigation infrastructure is essential, it’s not enough. Effective CSI also requires supportive policies, strong institutions, good water governance, secure land and water tenure, farmer knowledge, market access, and credit availability.

Introduction

Maize (Zea mays L.) is one of the most versatile promising crops having wider adaptability under diverse agro-climatic conditions. Worldwide, maize is known as “Queen of cereals” because it has the maximum genetic yield potential amongst the cereals. It is cultivated on nearly 197 million ha in about 170 countries having wider diversity of climate, soil, biodiversity and management practices that contributes 36% (1135 million tonnes) of the global grain production. The United States of America (U.S.A.) is the largest producer of maize contributes nearly 33% of the total production in the world and other important growing countries are China, Brazil, India, Argentina, Indonesia, Ukraine and Mexico (Table 1).

INTRoDUCTION

In developing countries, cereals and legumes are the principal source of daily energy and protein intake. The major economic loss caused by grain infesting insects in not always the actual material they consume, but also the amount contaminated by them and their excreta which makes food unfit for human consumption. Today million of tones of the food grains are damaged by the ravages of the pests that not only cause quantitative but also qualitative changes in food grains. Stored product insects depress the food value of a product, introduce pathogens, parasites or toxins or make it less palatable. Insect infestation in high in the tropics and may lead 100 per cent damage in severe cases.
 
In order to cope with the requirement of the increasing population and food problem, at present as well as for the decades to come, the world will have to raise agricultural production very substantially, both by increasing the production and by reducing losses on account of post harvest spoilage especially of cereals and legumes which are the primary sources of food for men kind. This is a major problems during agricultural production, food transfer and processing. Million tonnes of grain worth several hundred corers of rupees every years are either damaged or loss for want of knowledge of scientific methods of saving the foodgrains during storage. In India, post-harvest losses caused by the unscientific storage, rodents, insects, micro-organisms, moisture etc. account for about 10 per cent of total foodgrains (Anonymous, 1971). A world survey by F.A.O. indicated about 5 per cent loss of cereals, in storage annually (Herford, 1952).

Introduction

The various kinds of plants present on earth have been used over the millennia for human welfare in the promotion of health and as drugs and fragrance materials. About 80% of the world’s inhabitants rely mainly on herbal medicines for their primary health care, while medicinal plants continue to play an important role in the health care systems of the remaining 20%. The use of herbal medicine and medicinal plants in most developing countries, as a normative basis for the maintenance of good health, has been widely observed (UNESCO, 1996). According to WHO (1998), herbal medicines are finished, labelled medicinal products that contain as active ingredients, above ground or underground parts of plants or other plant materials, or combinations thereof, whether in the crude state or as plant preparations. The plant materials referred to above include juices, gums, resins, fatty oils, essential oils and any other substances of this nature. Herbal medicines may contain excipients in addition to the active ingredients (WHO, 1991). Remarkably, even today there is no real definition for this special group of plants that has been accompanying mankind throughout history. Most frequently, medicinal plants are defined as feral and/or cultivated plants that, based on tradition and literature records, can be directly or indirectly used for medical purposes. The basis of medicinal use is that these plants contain so called active ingredients (active principles or biologically active principles) that affect physiological (metabolic) processes of living organisms, including human beings.

The conception about aromatic plants is even less definite. The term aromatic indicates plants having an aroma; being fragrant or sweet-smelling, while the word aroma is supposed to imply also the taste of the material (aromatic herbs). Since, large number of plants possess both medicinal and aroma properties which leads to complexity and overlapping uses of active ingredients, making impossible to establish rigid categories or a practical classification for medicinal and aromatic plants. For example; Anise, dill, coriander, thyme, etc. are equally known as medicinal, spice and essential oil crops. Thus, frequently these plants are simply referred to as medicinal plants, disregarding their specific features. More recently, the term “Medicinal and Aromatic Plants” (MAPs) has been used in a slightly broader sense, distinguishing the fragrant (aromatic, ethereal) ingredients containing group of medicinal plants.

Introduction

It is already established that mathematical modeling aims to describe the different aspects of the real world, their interaction, and their dynamics through mathematics. It constitutes the third pillar of science and engineering, achieving the fulfillment of the two more traditional disciplines, which are theoretical analysis and experimentation. Nowadays, mathematical modeling has a key role also in aquaculture. The problem is the complex modeling other than counting. Modeling and reasoning with data of different kinds can get extremely complex. Data streams are common source of Big Data. Now the task is to collect, prepare, represent, model, reason, visualize and additional issues like usage, quality, context, streaming, scalability. A few publications are available on modeling of fisheries and aquaculture in Indian context with live examples of modeling (Roy, et al. 1998, 2002, Roy et al., 2002, Ghosh et al., 2017, Roy, et al., 2006, Roy et al., 2008, 2012). A book on exclusively with various types of model applicable in fisheries and aquaculture has been published (Roy et al., 2009). In all these earlier publications conventional statistical and econometrics techniques are applied. But today there is sea change in this field with the advancement of technologies like artificial intelligence, machine learning, and big data analytics. There are attempts to apply these cutting edge technologies aiming sustainable productivity through efficient management of resources. In the following sections an attempt has been made to present an overview of the advances in analytics along with a briefing on what was developed in the past, and what is available today. Before proceeding to the analytics, I am presenting the scenario of basic characteristics of data along with different types of data generated today with automation of aquaculture industry.

Understanding biology and genetics at molecular level has become very important for better understanding and manipulation of genome architecture and this creates new opportunities to streamline plant breeding. Breeding is simply defined as the selective mating of individuals of a population to isolate or combine desired morphological, physiological or genetic traits such as appearance, yield, and disease resistance. This is performed with the assistance of identifiable traits.

Molecular markers have contributed significantly in this respect and have been widely used in plant science in a number of ways, including genetic fingerprinting, diagnostics, identification of duplicates and selecting core collections, determination of genetic distances, genome analysis, identification of markers associated with desirable breeding traits which are useful in marker assisted breeding, genomics and development of transgenics. Use of these markers also significantly reduces breeding time and cycles required for crop improvement. This is reflected in the fact that complete molecular and genetic maps have now been constructed for at least 20 major crop species and many more have been characterized at less defined level and the genomes of a few model plant species were completely sequenced.

Introduction
Molecular systematics is a branch of systematics that uses molecular data, particularly DNA sequences, to understand the evolutionary relationships among organisms. It utilizes nucleic acid and amino acid sequence compositions to analyse the taxonomy, phylogeny, and biogeography of biological organisms. It offers a superior alternative to morphological systematics, which relies on physical traits. This approach has significant applications in taxonomic studies, disease diagnosis, biodiversity conservation, and industrial purposes. The integration of molecular biology and bioinformatics has significantly advanced the capabilities of molecular systematics, making it a powerful tool in both microbiological research and public health.

1. Introduction
Fish and fisheries remains to be an important sector for Indian economy providing food, nutrition as well as employment to a large share of the population. Initiating with a production of merely 7.7 lakh ton of fishes in 1951-52, the country was on its way of increasing the production steadily and has now attained the phenomenal success of producing 162.5 lakh ton during 2021-22. Of this total amount, 41.3 lakh ton has been obtained from marine fisheries and 121.2 lakh ton from inland resources (Anon, 2022). For the inland fisheries production, the contribution of fish culture sector was much lower than that from capture fisheries for several decades during the initial phases. However, the production of culture fisheries maintained a consistently increasing trend and has now surpassed in magnitude the production from capture fisheries sector (Fig.1). With the potential areas for fish culture being gradually used up for different purposes, maintaining such high production rates in coming days will be possible only through optimum management of various factors which govern the productivity of the ponds. Among such factors, one important aspect is nourishing the quality of pond soil and water.

Introduction

Oilseeds are the third most important group of crops after cereals and pulses in India. These are important food crops which also find their place in industrial use. Oilseeds have been an integral component of Indian agriculture since time immemorial. On the oilseeds map of the world, India occupies a prominent position, both in terms of acreage and production. India is the 4^th largest edible oil economy in the world and contributes about 10% of the world oilseeds production, 6-7% of the global production of vegetable oil, and nearly 7% of protein meal. The oilseeds production statistics of the world for the year 2016-17 includes a production of 549.98 million tonnes from an area of 234.57 million ha with the productivity of 2.34 tonnes/ha. The top countries include U.S.A., China and India with U.S.A. ranking first in all area, production and productivity of 39.25 million ha, 126.94 million tonnes and 3.23 tonnes/ha, respectively. Next is China which ranks 2^nd in production and productivity but 3^rd in terms of area; the area, production and productivity being 22.72 million ha, 54.92 million tonnes and 2.42 tonnes/ha, respectively. India ranks 2^nd in terms of area, i.e. 33.83 million ha but 3^rd in both production and productivity being 36.32 million metric tonnes and 1.07 tonnes/ha respectively.

Introduction

India has very old and long tradition of flower cultivation. With changing life styles and increased urban affluence, floriculture has assumed a definite commercial status in recent times. Improved communication facilities have increased their availability in every part of the country. Floriculture in India is being viewed as a rapidly growing industry. In India, at present total area under flower crops is about 2,33,000 ha with production of about 17.29 lakh tonnes of loose flowers and 76732 lakh  number of cut flowers (NHB database, 2013).The important floricultural crops in the international flower trade are rose, carnation, chrysanthemum, gerbera, gladious, gypsophila, liastris, bird of paradise, orchids, anthurium, tulip, lilies, etc. All the flowers and ornamentals are the most perishable commodities needs special care during harvesting, handling, storage and transport. The losses can be both, quantitative as well as qualitative, which result in loss in terms of monitory value.

Low bioavailability of phosphorus (P) in soils is one of the major limiting factors influencing crop production throughout the world. Plants, however, are known to possess potential adaptive mechanisms at morphological, physiological, biochemical, and molecular levels to overcome P deficiency (Vance et al., 2003). Such adaptive mechanisms mainly include an increase in total root length and root hair growth (Bates and Lynch, 2000), enhanced organic acid (Singh and Pandey, 2003), acid phosphatase (Pandey, 2006) and ribonuclease (RNase) secretion into the rhizosphere (Hocking, 2001), increase in expression of proteins such as phosphatase, inorganic phosphate (Pi) transporter, RNase and phosphoenolpyruvate carboxylase (PEPcase) in plant tissues.

INTRODUCTION
 
The form genus Sclerotium was described by Saccardo (Saccardo, 1913) to include fungi that produce sclerotia and had sterile mycelia. Several fungi were inc1uded in this genus, and all were characterized by production of small, dark brown or black spherical sclerotia that were internally differentiated into a rind, cortex and medulla. Several plant pathogenic fungi with this sclerotial type and with no known sexual or conidial state were placed in the genus Sclerotium. However, as the teleomorphic states were discovered, affinities to the Basidiomycotina (Punja, 1988) or Ascomycotina (Krause and Webster, 1972) were established for some members of the form genus.

Abstract

Potassium (K) is the third most essential macronutrient element for plants. More than 98% of K in agricultural soils are remained as insoluble organic and inorganic compounds or complexes that are unavailable for plant uptake. Nutrition of crop plants are heavily relied on application of expensive inorganic chemical fertilizers, which is a potential cause of environmental pollution. An eco-friendly alternative strategy for plant K nutrition could be the application of K solubilizing bacteria (KSB) as biofertilizer. Plant and soil-associated bacteria from diverse taxonomic genera such as Bacillus, Pseudomonas, Acidithiobacillus, Paenibacillus, Flectobacillus, Arthrobacter, Enterobacter, Sphingomonas, Paraburkholderia etc. have shown high K solubilizing activities and promote plant nutrition in K deficient soils. The mechanisms involved in their activities include acidolysis, chelation, exchange reactions, and enzymatic degradation of insoluble K minerals and other K complexes. However, inconsistency in the efficiency of KSB-based biofertilizer for crop production in diverse ecological settings is still a big challenge. Recent genomics, post-genomics and CRISPR-Cas genome editing approaches seem to be useful for a better understanding the underlying mechanisms of KSB and development of highly efficient K fertilizers for promoting sustainable agriculture. This chapter comprehensively reviews current updates of KSB-based biofertilizer and their potentials for promoting sustainable crop production. The role of genomics and post-genomics approaches for better understanding of the mechanisms of the functions of applied KSB-based biofertilizers in the crop field and mitigation of the existing inconsistency in the efficiency of KSB-based fertilizers are also discussed.

Tissue Doppler Imaging

Doppler echocardiography relies on detection of the shift in frequency of ultrasound signals reflected from moving objects. With this principle, conventional Doppler techniques assess the velocity of blood flow by measuring high-frequency, low amplitude signals from small, fast-moving blood cells. In TDI, the same Doppler principles are used to quantify the higher-amplitude, lower-velocity signals of myocardial tissue motion.

There are important limitations to TD interrogation. As with all Doppler techniques, TDI measures only the vector of motion that is parallel to the direction of the ultrasound beam. In addition, TDI measures absolute tissue velocity and is unable to discriminate passive motion (related to translation or tethering) from active motion (fiber shortening or lengthening). The emerging technology of Doppler strain imaging provides a means to differentiate true contractility from passive myocardial motion by looking at relative changes in tissue velocity.

Precision viticulture aims to optimize vineyard management, reduce use of resources, reduce environmental impact and maximize yield and quality of grapes. New technologies as UAVs, satellites, proximal sensors, variable rate machines (VRT) and robots are being developed and used more frequently in some parts of the world in recent years. Developments and abilities of computers, software and informatic systems to read, analyze, process and transfer a huge amount of data are major milestones in precision viticulture. In addition, different decision support systems (DSSs) for making better crop management decisions at the right time also assist vine growers.

Introduction

Pomegranate (Punica granatum L.) is an important fruit crop in arid and semi arid regions. In India, it is grown commercially in Maharashtra, Gujarat, Karnataka, Andhra Pradesh, Telangana, Tamil Nadu, Madhya Pradesh, and Rajasthan. India is one of the largest producers of pomegranate in the world. During 2020–2021, pomegranate was cultivated over 2.71 lakh ha with an annual production of 30.88 lakh tonnes and a productivity of 11.39 tonnes/ ha in India. Maharashtra is the leading state, with 63% of the area and 62% of total production. In India, pomegranate is available throughout the year (January–December). India has the largest area of pomegranate cultivation globally.

Pomegranate can tolerate heat, drought, and moisture deficit. The area under pomegranate cultivation in India is increasing daily due to its high demand, hardy nature, low cost of cultivation, high yields, better storage quality, and therapeutic values. It has enormous medicinal and nutritional value and is one of the richest sources of antioxidants. Several processed products such as juices, squash, jelly, anardana, and mouth fresheners are prepared by fruit processing. The fruit is mainly used as an ingredient in cooling and refrigerant mixtures and in the preparation of juice, concentrates, condiments, and pastes. The juice is highly nutritious and is recommended for patients suffering from gastric troubles. It contains 16.5% TSS and 0.33% acidity; total sugars, 13.93; reducing sugars, 13.60 and non-reducing sugars, 0.33%; and 9.27 mg/100 g ascorbic acid.

Introduction Beetroot (Beta vulgaris L.) is a very popular herbaceous biennial root vegetable mainly grown for its edible roots and leaves, it is commonly known as Chukander in Hindi, but also has different local names depending on the area, for instance, it is called Bita gacha in Bengali, Bita in Marathi, Beet in Punjab, Salada in Gujarati, Dumpamokka in Telugu, and Bit in Malayalam etc (Kumar, 2015). It belongs to the family Chenopodiaceae (now Amaranthaceae). The chromosome number of beetroot is 2n = 18. It is used as a cooked vegetable, salad, and for pickling and canning. Origin and distribution The origin of beetroot (Beta vulgaris) can be traced back to the Mediterranean region and the Middle East.

Introduction The popular legume crop known as broad beans, or Vicia faba in scientific jargon, is valued for its diversity and nutritional content. Broad beans have a long history that dates back thousands of years, and they have been used extensively in many cuisines and agricultural practises all over the world. With the use of references from reputable sources, this introduction will discuss the traits, cultivation, and health benefits of broad beans. Annual plants in the Fabaceae family, broad beans have a cool growing season. They originate from the Mediterranean region, but due to their resilience to various climatic conditions, they have been grown in numerous locations across the world. With a height of up to one metre, the plants are renowned for their vigorous growth. Large, podded fruits with many seeds or beans are produced by broad bean plants. Broad bean cultivation is a rather simple process

Introduction The ‘Brussels sprouts’ (Brassica oleracea L. var. gemmifera) coming under Brassicaceae family with chromosome number 2n=18 preferred by farmer for its edible green bud or sprouts (small heads that resemble miniature cabbages). It is a robust, slow-growing, longseason vegetable. The “sprouts” are formed in the leaf axils, beginning at the base of the stem and progressing upward. Brussels sprouts are high in vitamin A and C, as well as folic acid and dietary fiber. It also has anticancer effects due to the presence of sulforaphane. Similarly, to other Brassicas, Brussels sprouts contain indole-3-carbinol, which inhibit cancer cell growth. The temperate regions in the hills have the greatest potential for commercial production of brussels sprouts, followed by coastal regions with prolonged cool falls.

Introduction Cauliflower Brassica oleracea var. botrytis) is a cold season vegetable cropbelongs to family cruciferous or Brassicaceae family with somatic chromosome number 2n = 18. Cauliflower is derived from the Latin word i.e., ‘caulis’ means stem and ‘floris’ means flower and wild progenitor is Brassica cretica. In India, cauliflower is grown in two different varieties: snowball type and tropical type. However, at first, other varieties of cauliflower, such as the European and Cornish types from England, were also brought to India. Later, the Cornish and European forms of cauliflower were intercrossed to develop the tropical type of cauliflower. The Indian type or tropical type of cauliflower can be grown in hot weather and high rainfall condition therefore, such type of cauliflower is mostly grown in Indian plains during the month of May to January followed by temperate type or snowball group based on the temperature based varietal classification.

Introduction Chinese cabbage (Brassica compestris var. pekinensis i.e., heading type and Brassica compestris var. chinensis i.e., non-heading type) with chromosome number 2n=20 is a green leafy vegetable that has come under the same family Cruciferae as cabbage. It is also called as celery cabbage, Bok choy, Peking cabbage, snow cabbage, napa cabbage etc. according to the places. It is originated from China and later adopted to other countries, mostly in Asian countries. Even though not being a member of cole group, it is regarded as one because of the similarity in cultural practices, breeding systems, same growing season and many other features that resembles apart from being a brassica vegetable. The best planting season for Chinese cabbages is in early spring to harvest as a late spring and after mid-summer for a fall crop. The inner leaves of the plant are light yellow colour with long (oblong) shaped head which is covered by compact wrinkled, profuse and light green leaves. Generally, the crop has high nutritional value due to which its demand is increasing tremendously. The potassium content of the crop is high with low fat content. It is also rich in vitamin C, fiber, folate as well as beta carotene and calcium are also more as compared to the cabbage due its leaves colour

Introduction Garlic (Allium sativum L.) belongs to the monocotyledonous family Alliaceae or Amaryllidacea; Chromosome No. 2n=2x=16. In India, it is the second most extensively grown bulb crop after onion. According to several botanists (Candolle, 1985; Dscalov and Popov,1941; Kuznetsov, 1948 and Regel, 1975) garlic was cultivated and utilised by Mediterranean population since medieval time. Origin, History and Distribution Central Asia, particularly elevated highland of Pamir and Tian Shan is considered as the primary centre of origin of garlic (Vavilov, 1926), however Caucasus and North Africa is considered as the secondary centre of origin. Allium longicuspis is considered as the wild ancestor of garlic. The history of garlic usage dates back to 3000 B.C when it was used in Egyptians tombs likewise its first usage in modern era occurred during 10th century in England (Shinde and Sontakke, 1986) and its first introduction in North and South America was done by Spanish and Portugese settlers during fifteenth century; introduction and usage of garlic in other European and Asian countries occurred subsequently.

Introduction The Globe artichoke is a herbaceous perennial crop that is grown for its tender, edible, immature flower buds. It is botanically known as (Cynara scolymus L.) and having chromosome number 2n=2x=34. The globe artichoke belongs to the family Compositae, and it originates in the Mediterranean Basin, where it was most likely introduced as a crop in about the first century AD (Sonnante et al., 2007). The globe artichoke is presently grown as an annual crop or a perennial crop in different regions of the world. The increasing demand for globe artichokes as a source of pharmaceuticals and modern functional foods has created new challenges for producers, necessitating the concurrent development of new production hubs and the use of cutting-edge technology in established growing regions that can support the global market.

Introduction Kale (Brassica oleracea var. acephala) belongs to family Cruciferae or Brassicaceae is a under exploited vegetable crop. It is introduced in 19th century. Economic Importance and Uses Its edible part is leaves and contains amazing source of amino acids, vitamins and minerals which has many medical advantages. It helps in lowering of blood cholesterol level which helps in lessening the heart illness. Kale leaves is also rich source of beta carotene, ascorbic acid and phylloquinone. Kale is a nutritious vegetable that is rich in vitamins A, C, and K, as well as calcium and iron. It is also a good source of antioxidants and fiber, making it a popular choice for health-conscious consumers. Kale is not so popular crop in India therefore it confined to very few states of Northern India like HP, UP, J & K, Pb and Haryana.

Introduction In India, knol-khol (Brassica oleracea var. gongylodes) is belongs to family brassicaceae with chromosome number is 2n=18. It is grown in north eastern states of India, J &K, West Bengal, Assam, Punjab, and other regions of southern India also. It is distinguished by the formation of knobs(tuber) is a thickening of the stem tissue above the cotyledons. This knob is picked for human use as a raw or cooked vegetable, while young leaves are also utilized in some areas. Economic Importance & Uses Knol-khol, also known as kohlrabi or German turnip, is a biennial vegetable that belongs to the same family as cabbage and broccoli. It is a popular crop in many parts of the world, including Europe, Asia, and the Americas.

Introduction The leek (Allium ampeloprasum var. porrum L.) belongs to the Alliaceae family and is a non-bulb-forming crop. It is consumed raw by itself, combined with other foods in salads, and cooked as flavoring in soups and stews. It is a preferred vegetable in a kitchen garden but is not commercially farmed in India. Leek has chromosome 2n=4X=32, which makes it an autotetraploid in nature. It is a vigorous biennial herbaceous cultivar that has never been discovered in its wild form. For their blanched stems and leaves, leeks are grown. It is a biennial plant that is used to flavor soups and eaten as a salad. The leek was first domesticated in the Eastern Mediterranean, where it was later brought to middle Europe by the Greeks, Romans, and Egyptians. Leeks may have originated in Central Asia and/or Europe, according to several theories. Since the majority of the Allium species are found worldwide, their distribution within the northern hemisphere is not uniform.

Introduction The Asteraceae family includes Lactuca sativa, better known as lettuce, which is cultivated as a leafy vegetable. Due to its usage in the creation of salad, soup, and vegetable curries, this plant is well-known around the world. Additionally, this herb has fantastic therapeutic qualities. Because of its flavor and excellent nutritional content, lettuce is one of the most popular raw green leafy vegetables and is grown all over the world. It is thought to be a crucial source of phytonutrients. Significant physical and genetic differences define it. The Egyptians were the first people to plant this leafy crop. It is currently manufactured all over the world and used in forensic medicine to treat a variety of conditions, including as pain, inflammation and issues with the stomach, as well as urinary tract infections. Its pharmacological potential, which includes antibacterial, antioxidant, neuroprotective, and hypnotic actions, has been scientifically supported by a number of research.

Introduction The botanical name of carrot is Raphanus sativus which belongs to the family Brassicaceae having haploid chromosome number of x = 9 and is believed to be originated from Europe and Asia. Radish is one of the most ancient and popular leaf cum root vegetables in both tropical and temperate regions. Radish probably originated in Europe and Asia. Radishes were extensively cultivated in Egypt and ancient records show that radishes were eaten before the pyramids were built. It had spread to China during 500 BC and to Japan during AD 700. The recent study has indicated that east of Mediterranean region, most probably China is the centre of origin of radish. Wild state of radish is not known. Previously it was believed to haveevolved from a common weed Raphanus raphanistrum Linn in Europe but now it is believed to have been evolved from more a variety of sources. As it is a short duration annual or biennial crop, it can be widely used in sequence cropping, companion cropping and intercropping in widely spaced crops. Roots are pink in colour due to presence of anthocyanin pigment.

Introduction The “rhubarb and pie plant,” also known as Rheum rhaponticum L., is a member of the Polygonaceae family. Diploid (2n = 2x = 22), tetraploid (2n = 4x = 44), or hexaploid (2n = 6x = 66) rheum species are the three possible forms. The long, thick leafstalk, often called the petiole, is used to make pies and sauces. The rhubarb stalks’ colour may range from the typically recognized crimson red to pale pink with specks to plain light green. Rhubarb is a perennial plant that thrives in cold climates and is drought and winter tolerant. Edible petioles and leaves are the first springtime shoots to emerge. rhubarb was first cultivated in Italy and introduced to the continent in the year 1608. The earliest recorded use of rhubarb in the United States was in 1778, but by 1806 it had achieved broad acceptance. According to Lindley’s Treasury of Botany, the name comes from the Greek word rheo, meaning “to make one vomit,” because of the root’s laxative properties. It is a good source of carbohydrates (3.8g), protein (0.74 g), fibre (1.75 g) and fat (0.13g) and minerals like calcium (130 mg) and potassium (360 mg) per 100 grams of edible portion. There are numerous rhubarb cultivars contain bioactive substances such as flavonoids, anthraquinones, glycosides, tannins, volatile oils, and saponins.

Introduction Spinach, scientifically known as Spinacia oleracea, is a versatile leafy green vegetable that is widely consumed and enjoyed for its nutritional benefits and culinary uses. Believed to have originated in Persia (modern-day Iran), spinach has been cultivated and consumed for centuries in various regions of the world. It is highly regarded for its vibrant green leaves and is a popular ingredient in salads, soups, smoothies, and cooked dishes. Spinach is an annual plant, meaning it completes its life cycle within one year. It has an upright growth habit with an erect, branched stem that can reach a height of about 30-60 cm. Due to its delicate flavor and versatility, spinach is used in various culinary preparations. It can be consumed raw in salads or added to sandwiches for a fresh and nutritious crunch. Spinach is commonly cooked as well, either sautéed, steamed, or included in soups, stews, and stir-fries. Its mild taste makes it an adaptable ingredient that complements a wide range of dishes (Thind et al., 2021)

Introduction Broccoli (Brassica oleracea L. var. italica) having chromosome number 2n=2x=18 is a native of Mediterranean region. The broccoli was developed in eastern Mediterranean region and Italy is the main diversification which was later introduced into England about 1720. It is a cool season crop which produces fleshy green flower heads arranged on branches sprouting from thick edible stalk in such way that it looks like a miniature bonsai tree. It has the same family as cabbage and cauliflower. Even though it resembles cauliflower by forming head like structure but it has longer flower stalks with loose large green terminal head (10 to 20 cm). In Italy broccoli is called as broccoli which means “the flowering crest of a cabbage.” Due to the presence of bountiful nutritious properties, it is also known as “Crown Jewel of Nutrition”. It is a great source of vitamins A, B and C and minerals like iron, fibre, calcium, zinc, magnesium etc. Apart from the minerals and vitamin it also a rich source of riboflavin, thiamine and niacin.