eChapters

Introduction

The protozoa (meaning proto- first and zoa mean animals) are considered to primitive and are unicellular organism. The protozoa are Eukaryotic organism having nucleus in which the genetic information is stored in chromosomes of nucleus. In poultry, the protozoan disease categorized into two main group

1. Intestinal protozoan parasites include Eimeria spp. (Coccidiosis), Cryptosporidia (Cryptosporidiosis) Histomoniasis (Histomonas meleagridis), Trichomoniasis (Trichomonas spp.) and Spironucleus (Hexamitiasis).

Coccidiosis is important disease of poultry caused by protozoa of the genus Eimeria. The disease occurs by ingestion of sporulated oocyst ehcih upon action of trypsin rupture in gizzard and proventriculus relealeses sporozoites. The sprozoites penetrate mucosa of intestine and two generation of asexual reproduction occurs (Schizogony) and releases merozoites which mature and produces gamonts. Sexyual reproduction occurs (Gametogony) and produses zygot which mature to form oocysts. The oocysts are passed in faeces. The high moisture (exceeding 30%), immunosuppressive diseses and environmental and managemental stress lead to occurrence of disease. The birds affected with coccidiosis show depression, ruffled feathers, blood tinged diarrhoea and pale comb and wattle. Specific part of intestine is affected by each species of Eimeria. Diagnosis of coccidiosis is done by finding oocyst in faeces under microscope.

A mutagen is a physical or chemical agent that changes the genetic material (DNA) of an organism and thus increases the frequency of mutations above the natural incidence. Errors during DNA replication, repair and recombination also causes spontaneous mutations naturally. Mutagens may be of chemical, physical or biological origin. The properties of each mutagen vary by causing damage to the DNA, and most often result in replication error.

Q fever (query fever) is an important global occupational zoonotic disease caused by a bacteria Coxiella burnetti. It is an OIE notifiable disease and showing an emerging and reemerging trend in different parts of the world. It is having great public health importance as the pathogen is transmitted through almost all routes but predominantly by aerosols from reproductive discharges of animals of farm and pet animals which serve as main reservoir of infection. The organism is shed in milk and foods of animal origin for very long period. Q fever is associated with a wide clinical spectrum and most of its acute infections are either asymptomatic, mild or resemble to influenza. Presently, the infection is endemic in many areas and its prevalence has been confirmed in at least 51 countries including India.

Etiology: C. burnetti was classified as a member of Rickettsiaceae family however, recent scientific investigations on 16S RNA based sequence analysis have shown that it is genetically more comparable to bacterial families covering genus Francisella and Legionella therefore it has been reclassified in the family Coxiellaceae or order Legionellales. It is a Gm-, pleomorphic rods (0.2-0.41μX0.4-1.0μ) or sphere (0.3μ-0.4μ in diameter in size) and an obligate I/C organism. It is relatively resistant to physical and chemical agents. The bacterium occurs in two antigenic forms - the pathogenic form (phase I) found in infected animals or humans and the avirulent form (phase II) obtained by repeated passagein embryonated eggs or in cell culture. Phase II cells are autoagglutinable and are readily phagocytosed in the absence of specific antibodies. Phase I activity is attributed to surface carbohydrate antigen which is soluble in trichloracetic acid and is destroyed by periodate. Phase I is a more powerful immunogen than phase II and elicits antibodies in high titer to both antigens. Phase II antigen gives better results in CFT with human and animal sera. Q fever sera do not react with other rickettsial antigens or with Proteus. In nature, bacterium exists mainly in 3 forms, namely - the small cell variant (SCV), the small dense cells (SDC) which are spore like forms that remain very stable in the environment as extracellular infections particles and the large cell variant (LCV) which is the growing and metabolically active form inside the host. In view of the potential for transmission of this infection from animal to people, the disease has been classified as a notifiable OIE disease.

1    Consent form for surgery

2    Consent form for euthanesia

3    Slaughter certificate

4    Wound certificate

5    Proforma for export certificate of dogs

Even after public concern the scientists feel that the GMO foods are quite safe. According to them the genetically-engineered crops are very safe to eat as much as the non-GE of the same crop. According to them the environment has improved by reducing the use of pesticides. GMO stands for genetically modified organism. Soy, corn, or other crops are very common as GM crops which are made by genetically engineered DNA. Many have come to the conclusion that GM foods are safe, healthy and sustainable. However, some people are against them. There are many benefits of GM crops like less water or pesticides to be used. Genetically engineered crops fight diseases to save the foods. At the same time the industries/ farmers that grow them are also benefitted. These foods have higher nutritional profiles, both in the US and abroad like corn which is one of the most prominent GMO foods. Soy is found in tofu, vegetarian products, soybean oil, soy flour and many other products. The other major crops taken for genetic modification are Papayas, Canola and Cotton. For dairy products a lot of work is being done on the genetic engineering of animals in a big way. This is going to be the most intractable environmental challenge of the 21^st Century. Presently, around 92% of the U. S. corn (GE), 94% of soybeans and 94% of cotton are genetically engineered.

Introduction

Aonla or Indian gooseberry (Emblica officinalis Gaertn. Syn. Phyllanthus emblica) is one of the important indigenous fruits of the Indian subcontinent. Most of the xerophytes are members of the Euphorbiaceae.

The tropics are evergreen, while the subtropics are deciduous. The highest concentration of vitamin C in aonla is found in mature fruit rather than immature fruit. The special attribute is its capacity to retain vitamin C even in a dried stage, which is not possible in other fruits.

Aonla contains six seeds per fruit, and in it is found zygotic dormancy. Flowers are borne in the axils of the leaves on determinate shoots.

Indian Government has several developmental programmes to promote agriculture and rural sector. It includes Drought Prone Area Program, Antodaya Programme, Integrated Rural Development Programme, Hill Area Development Programme, Desert Development Programme, Intensive Jawahar Rojgar Yojna and Employment Assurance Scheme, Indira Awaas Yojna, Integrated Waste Land Development Programme, National Food Security Mission, National Horticulture Mission, etc. Mahatma Gandhi National Rural Employment Guarantee Scheme (MGNREGS) has the main focus to provide guaranteed employment for 100 days in a year to every rural household. Since 2014, the Swachh Bharat Abhiyan has been a major initiative by the Government of India.
THE FOOD SECUR3ITY BILL
The Food Security Bill is here. Some of the salient features are
Nearly 82 crore (820 m) poor people to get food grains at highly subsidized rate, nearly 20 crore poor in urban areas and 62 crore in rural areas will be eligible to get 5 kg rice, wheat and coarse grains at Rs.3, 2 and 1 respectively. Additionally for pregnant women, there will be a provision for free food for 10 months and for children below 7 years, food will be free. There is one danger point feared in this scheme that in the long run, many farmers will stop farming and go for easier way for casual labour and take food security cover in stead of farming the low production lands. But on the other hand we should look at the solution to this keeping in mind that the Food Security Bill is here and how best we can build on our strength rather than weakness. The areas of strength to look for should be :

Heat stress induced by climate change is a serious issue of livestock industries worldwide (Bajagai, 2011). When environmental temperatures move out of the thermo-neutral zone (or comfort zone) animals begins to experience either heat stress or cold stress. Both these stresses require the animals to increase the amount of energy used to maintain the body temperature so that less energy is available for productive processes. Atmospheric temperature, relative humidity, air movement and solar radiation are the major factors that determine the heat load on animal. Thermoregulation is the ability of the animals to maintain their body temperature in cold or hot environments. It consists of behavioural, physiological and anatomical responses that affect energy metabolism. At lower temperature as a physiological adjustment to maintain the body temperature the animal increases the metabolic heat production by increasing the basal metabolic rate and uncoupling of oxidative phosphorylation. Hence the maintenance energy requirement of animals increases in a cold environment, which reduces the amount of energy available for production. From a practical point of view higher temperatures are much more dangerous for producing animals than a cold environment. Temperatures exceeding the higher critical level compromise animal performance not only by changing the energy and nutrient metabolism, but also by upsetting the body homeostasis, with detrimental consequences both for immune competence and for product quality. In general, livestock with high production potential are at greatest risk of heat stress, and require most attention.

Due to the globalization of the food supply there has been a need to strengthen food safety systems in and between the countries. In this regard the World Health Organization (WHO) has promoted efforts in improving the food safety from farm to plate which started on World Health Day, 7 April 2015. The World Health Day 2015 slogan was “From farm to plate, make food safe”. The informal food production and marketing system is still strong in most countries of the South-East Asia Region, for the enforcement of food safety regulations. Traditionally, the preferences have been for hot and well-cooked food. These habits are partially responsible for preventing foodborne infections. In many countries street food has become very popular as it is cheap. Hygienic conditions are improving, provided that potable water supply and clean facilities are ensured by municipal authorities. Many waterborne and diarrhoeal diseases could be prevented with the introduction of bottled drinking water. The awareness and education on food safety by the consumers has strengthened in the enforcement of food standards. This has improved the hygienic practices and has helped in the prevention of foodborne illnesses. The “WHO Five keys to safer food” serves as the basis for educational programmes to train food handlers and educate the consumers. These help in preventing the foodborne illnesses. The important points are to keep food surfaces clean, washing of all utensils, plates and cutlery, keeping the raw food separate from the cooked food. Cooking food thoroughly to the appropriate temperature, keeping food at safe temperatures, both for serving and storage and using safe water and raw materials.

It is supposed that man was evolved on earth about 15 lakh years ago. This man started to move by standing erect on his feet and called Homo erectus L. Later the modern man was evolved called Homo sapiens L. (Sapiens means learning habit) due to his continuous learning habit. It is difficult to trace the exact period of beginning of agriculture. Archeological evidences indicated that, Agriculture started about 50,000 years ago.

Hunting was the primary source of food in olden days and was prevailed for long time. They obtained his food through domestication of some animals like dogs, horse, cow, buffalo etc. They live in the periphery of the forest and migrate from one place to another in search of food. It was not comfortable and they started enjoying by settling at one place near the river and started agricultural system.

Radiation Chemistry is a branch of nuclear chemistry that deals with the study of chemical effects of radiation on matter.

Radio activity is the process by which an atomic nucleus of an unstable atom loses energy by emitting ionizing radiations (a, b, g radiations). It may be of

Introduction

Persistent income from agriculture is most important for sustaining the farming sector. Conservation agriculture (CA) is a resource-saving agricultural production system that aims to achieve production intensification and high yields while enhancing the natural resource base through compliance with three interrelated principles, along with other good production practices of plant nutrition and pest management (Abrol and Sangar, 2006). Conservation agriculture is largely promoted as one of the few win–win technologies affordable to farmers, in the sense that potentially it improves farmers’ yields (in the long term) at the same time conserving the environment. CA is described by FAO (http://www.fao.org.ag/ca) as a concept for resource saving agricultural crop production which is based on enhancing the natural and biological processes above and below the ground. As per FAO definition CA is to a.) Achieve acceptable profits, b.) High and sustained production levels, and c.) Conserve the environment. It aims at reversing the process of degradation inherent to the conventional agricultural practices like intensive agriculture, burning/removal of crop residues. Shifting from tillage-based agriculture to no-tillage CA systems removes unsustainable elements in the current tillage based systems and replaces them with CA elements that make the production systems profitable and ecologically sustainable. Conservation agriculture offers an opportunity for arresting and reversing the downward spiral of resource degradation, decreasing cultivation costs and making agriculture more resource – use-efficient, competitive and sustainable.

The goats play a vital role in the economy of the poor and marginal farmers of rural India. Notwithstanding the fact that the goat population has shown a steady growth over the year’s very little effort has been taken for genetic improvement at farm level. As per 20th Livestock Census, 27.74% of the livestock population is Goats. Out of 148.88 million Indigenous Goats (Including Non-descript), 27.4% are pure breed, 9.1% are graded breeds and the remaining 63.5% are non-descript breeds. So, to increase the productivity per goat, we need to improve the breed quality scientifically. Artificial insemination (AI) using frozen semen is practiced to produce superior progeny and to accelerate the up gradation of stock. The conception rate with AI is lower but it has great potential to multiply superior quality of goat with faster rate. This technique also spread elite genetic material throughout a population with increased rate of genetic improvement. This technique is also important for the breed conservation process and has paved the way for other reproductive biotechnologies. The sufficient elite germplasm of male buck is not available throughout the country to cover breeding programmed by natural mating. AI is the best solution to improve the quality and productivity per goat.

1. Determination of qualitative nursery planting materials

1.1 Quality assessment of seedlings

Nursery is a place where young/infant seedlings are kept under intensive care until they are ready for planting. The production of quality seedlings begins with the collection of quality seed, nursery establishment, and after-germination maintenance. The quality of plant propagation structures may not be a qualitatively gradable criterion, but it can be indirectly assessed by their relationship to growth, productivity, and vigour. The Dickson Quality Index (DQI) is very useful for assessing the quality of a specific group of seedlings. This is done by selecting some seedlings at random from that group and computing Dickson Quality Index (DQI).

In the present investigation, nine cyanobacterial species viz. Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, Nostoc punctiforme, Nostoc linckia, Nostoc commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were tested for their activity pattern against some selected bacterial species.
 
Each cyanobacterial species was initially subjected to determine the early stationary phase of their life-cycle, which is believed to be the most appropriate period for the production of secondary metabolites by an organism. The growth curve for each cyanobacterial species was prepared by culturing them in BG-11 growth medium. Optical densities (OD) recorded at 650 nm were plotted against number of incubation days for each cyanobacterium. After 23-25 days of incubation period, each culture was harvested to obtain the biomass and spent medium, which were then subjected to process of extraction by using various solvents viz., Hexane, ethyl acetate, dichloromethane and methanol. The extracts and spent medium thus obtained were then investigated for antibacterial activity against five pathogenic bacteria including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnumoniae and Salmonella typhi.

The male genital system consists of:

1. The pair gonads, the testes
2. Epididymis
3. Vas deference or ductus deference
4. Accessory glands (Seminal vesicles, prostate glands, & bulbo-urethral glands)

Introduction

Bees belonging to the Apis genus can be found in a wide range of climates worldwide. Among them, the Apis mellifera Linnaeus species is distributed across diverse environments such as sub-Saharan Africa, northern Europe, and Central Asia. This species exhibits remarkable adaptability, thriving in various habitats like the oases of the African desert, the Alps, the edges of the tundra, and the misty regions of the United Kingdom. Additionally, other honey bee species within the Apis genus are predominantly distributed in Asia, specifically in tropical Southeast Asia.Honeybees are good biological indicators.

1. Genomic selection and CRISPR technology
• The adoption of genomic selection (GS) and CRISPR-Cas9 technology in spice breeding is likely to accelerate. These technologies can significantly improve the precision of breeding programs, enabling the development of varieties with enhanced traits like disease resistance, yield, and quality.
2. Climate-resilient varieties
• With climate change impacting agriculture globally, there is a growing trend towards breeding spices that are resilient to extreme weather conditions. This includes the development of drought-resistant, heat-tolerant, and
flood-tolerant varieties.
3. Biofortification
• Breeding programs are increasingly focusing on biofortification, which involves increasing the nutritional value of spices. This could lead to spices that are richer in essential nutrients like vitamins, minerals, and antioxidants.
4. Digital phenotyping and AI
• The integration of digital phenotyping tools and artificial intelligence (AI) is expected to revolutionize spice breeding. These technologies can enhance the efficiency of breeding programs by providing real-time data analysis
and predictive modeling.
5. Sustainable breeding practices
• There is a rising emphasis on sustainability in agriculture. Breeding programs are likely to incorporate practices that reduce environmental impact, such as using less water, reducing pesticide usage, and developing
organic farming-compatible varieties.

Since many decades environmentalists have warned of perils of chemicalised sports grounds, golf courses and cricket fields. They warn that our health is inextricably linked to the widespread use of toxic chemicals and also complain about the non-availability of safe alternatives.
Eco-sustainable sports fields must use the methods
• That do not pollute
• That are environment-friendly and effective
• That economize on the use of water and energy
Organizations like ECOCERT have prepared guidelines for Eco-sustainable fields including guidelines to take care of soils and turf, use of water including recyclation and use of only eco-friendly and certified compost and organic fertilizers.
Typical Golf Courses are heavy users of pesticides and water. UNESCO World Water Development report found that an 18 hole golf course can use as much as 2.3 million liters of water every day. With the increasing awareness and concern about health and environment, the demand for eco-friendly sports fields are on the rise.
Phil Weaver – Chairman of the Professional Golfer Association (PGA) has stated that “we are keen to have a more environmental approach to the golf industry. I am not sure organic is totally achievable but it is a great aim”. A numerous environmental certification system have been set up for golf courses that lead the way in terms of chemical reduction there are many limitations and difficulties. Some courses are making great sides, with recycling systems, minimum or no chemical use and alternative bio inputs. In BOX – 1 & 2 such examples are given which are typical.

Mutation in M₂

The output of mutations in M₂ and subsequent generations solely depends upon the complex interaction between many factors in the treated organism together with the specific modes of action of particular mutagen. These results are expressed quantitatively by the mutation rate and qualitatively by the mutation spectrum. The proportion of mutated plants to normal ones which is the mutation rate can be computed on the number of mutations per 100 M₁ plants (Gustafsson, 1940), number of mutations per 100 M₁ spikes (Stadler, 1928), and number of mutants per 100 M₂ plants (Gaul, 1960). After comparing the three indices, Gaul (1960) has concluded that mutants per 100 M₂ plants provided the best index, as it is proportional to the initial mutation rate and is independent of variations in progeny size and size of mutated sector. In Pisum, Blixt (1966) has confirmed this. Mohan Rao (1972) has reported that in barley, the M₂ seedling method determined the total induced mutation frequency. This method is also superior since it accounted for the components of induced mutation frequency viz., the frequency of mutated initials in each spike primordium and the frequency of spike primordia mutated.

The qualitative output of mutation in M₂ and later generations is expressed as the mutation spectrum which reflects the different frequencies of different mutations. Westergard (1960) found that the alkylating chemicals induced a higher proportion of less drastic mutations such as Viridis compared to extreme mutations such as Albina than those induced by radiations because of their apparent slight effect on chromosomes. Sidorova (1966) found a positive correlation between the spectrum of chlorophyll and the spectrum of morphological mutations.