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The biofilm formation is usually assayed by using Congo Red Agar (CRA) plate test. According to a method developed by Freeman et al., (1989) the isolates are stored in nutrient agar slants and screened for biofilm formation ability using CRA plates. Biofilm forming strains are observed as dark black colonies while others as red colonies on the CRA plates. Recently, the concept of these biofims have changed with new imaging technologies, biochemical methods and molecular ecosystem biology tools. A biofilm structure can be observed in 3-D form to the nano-scale level (Neu and Lawrence, 2015). A lot of studies are being conducted on the physiology of the biofilm cells, the genotypic and phenotypic variation among the biofilm community, as well as the biofilm metabolome, proteome and transcriptome (Raes and Bork, 2008). This will help in the the development of efficient methods to control deleterious biofilms (clinical biofilms, food contaminants, biofouling on industrial equipment and on ship hulls) and to enhance and modulate beneficial ones

India is regarded as a horticulture paradise due to growing of tropical, subtropical and temperate fruit crops in varied soil and climatic conditions. However, increasing pressure on land owing to diversion of orchard lands to various other obvious reasons as well as rising energy and land-costs, together with mounting demand for fruits have made HDP imperative to achieve higher productivity from limited space.

There is a shift in farmers’ perception from production to productivity and profitability, which can be achieved through high density planting. India is the second largest producer of fruits in the world. However, the average productivity of various fruit crops in the country is quite less. Low productivity can be attributed to many reasons such as old and senile orchards, lack of canopy management practices, planting of poor yielding varieties, low or no adoption of recommended package of practices by the farmer leading to poor orchard management, etc.

Sexual behavior

The pattern of courtship, display, motor activities and postures are directed to bring the male and female gametes together to ensure fertilization and pregnancy. The components of copulatory patterns are sexual arousal, courtship (sexual display), erection, penile protrusion, mounting, intromission, ejaculation, dismounting and refractoriness. The duration of courtship and copulation varies with the species both events are shorter in cattle and sheep than in swine and horses. The males sniff and lick the genitalia of female are more common and an important way of chemical communication through olfaction. Except in swine, the males smell the female’s urine and then raise head, with lips curled, in the ritualized “Flehmen’s reaction”. In sheep, goat and cattle, tactile stimulation of the female is made by nuzzling and licking the perineal region, whereas with the horse, the stallion often bites the female’s neck and in swine, the boar noses her flanks.

Canning is a process of sealing food stuffs hermatically in containers and sterilizing them by heat for long storage is known as canning.

Principle of Canning

Destruction of spoilage organism within the sealed container by means of heat.

Selection of Fruit and Vegetable

For canning fruit should be ripe but firm and uniformity mature and vegetables except tomato should be tender.

Grading

Selected fruit and vegetable are graded according to size and colour to obtain uniform quality. This is done by hand or machines such as screw grader and roller grader.

The biological diversity of each country is a valuable and vulnerable natural resource. Sampling, identifying, and studying biological specimens are among the first steps toward protecting and benefiting from biodiversity. Knowledge about biodiversity and the ability to identify organisms that comes with it are global public goods. That is, this knowledge can create benefits for all countries - benefits that no one country can create by itself.

DNA Barcoding: Documenting Biodiversity with a Gene Sequence

“DNA barcoding” is an exciting new tool for taxonomic research. The DNA barcode is a very short, standardized DNA sequence in a well-known gene. It provides a way to identify the species to which a plant, animal or fungus belongs. The Consortium for the Barcode of Life (CBOL) is promoting international partnerships that will enable people in all countries to better understand and protect their biodiversity.

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism’s mitochondrial DNA to identify it as belonging to a particular species. It is based on a relatively simple concept: most eukaryote cells contain mitochondria and mitochondrial DNA (mtDNA) has a relatively fast mutation rate, which results in significant variance in mtDNA sequences between species and, in principle, a comparatively small variance within species.

Soil is the largest terrestrial ecosystem where a wide variety of relationships exists between different types of soil organisms. The associations existing between different soil microorganisms, whether of a symbiotic or antagonistic nature, influence the activities of microorganisms in the soil. Microflora composition of any habitat is governed by the biological equilibrium created by the associations and interactions of all individuals found in the community. In soil, many microorganisms live in close proximity and interact among them-selves in a different ways. Some of the interactions or associations are mutually beneficial, or mutually detrimental or neutral.

The oxygen content may be measured either chemically or electronically. The normal chemical method is the so-called Winkler method which is a titration method, consisting of adding certain chemicals to the water and observing a colour change which will be directly related to the oxygen concentration.

Insects development

Once an insect hatches from the egg it is usually able to survive on its own, but it is small, wingless, and sexually immature. Its primary role in life is to eat and grow. If it survives, it will periodically outgrow and replace its exoskeleton (a process known as molting). In many species, there are other physical changes that also occur as the insect gets older (growth of wings and development of external genitalia, for example). Collectively, all changes that involve growth, molting, and maturation are known as morphogenesis.

Metamorphosis

• Metamorphosis actually means change in form Metamorphosis is derived from Greek word ‘Meta’ = Change, ‘morph’ = form or structure.

• The changes in the development from egg to adult, collectively called Metamorphosis (life cycle).

• Post-embryonic changes in form are collectively called as metamorphosis.

• Metamorphosis refers to the way that insects develop, grow, and change form. Metamorphosis include three developmental processes namely growth, differentiation and reproduction which takes place in larval, pupal and adult stages, respectively.

• Majority of insects pass through profound metamorphosis. The growth of an immature insect is accompanied by a series of molts or ecdysis, in which the cuticle is shed off and renewed. The number of molts varies in most insects from 4-8. However some undergo as many as 20 or more molts. A few insects like Bristle tail silver fish (apterygota) may continue molting even after reaching the adult stage. But most of the insects neither molt nor increase in size once then adult stage is reached. The intervals between the ecdysis are called Stadia. The term instar is applied to form of an insect during stadium. In numbering the instar, the form assumed by the insect between hatching from the egg and the first post embryonic molt is termed “First Instar”.

Man has tendency to classify everything in a hierarchical manner for example, ships may be classified on size, weight, what they carry or naval versus merchant or passenger. Any of these classification is as valid as any other.

But classification of biological species is unusual in that a predefined system exists i.e. the evolutionary relationships among organisms. But evolutionary relationships can only be estimated; and contrary to popular belief, even a good fossil record does not conclusively show the phylogeny or evolutionary tree of a group species.

Numerical taxonomy can be defined as “Numerical evaluation of the affinity or similarity between taxonomic units and ordering of these units into taxa on the basis of their affinities”. The term includes drawing of phylogenetic inferences from the data by statistical or other mathematical methods to the extent to which this should prove possible. The practice involves a number of fundamental assumptions and philosophical attitudes toward taxonomic work.

Fruit trees like ber, aonla, jamun, tamarind, custard apple, bael, lasora, phalsa, ker, pilu, etc well suited for arid environment, remained under exploited owing particularly to lack of appreciation of their quality as fruits, their economic potential and above all due to non-availability of the needed technology for their profitable cultivation. Because of their curative properties, these fruits have been used in Indian system of medicine such as Ayurvedic and Unani since time immemorial. Apart from their nutritive and medicinal values, quite a few of these underutilized fruits have excellent flavour and very attractive colour. In spite of these quality attributes, most have not undergone any conscious phase of domestication and human selection. Their cultivation is very restricted and they grow mainly wild. Being tolerant to biotic and abiotic stresses, these fruit species are suitable for growing in the disaster- and drought-prone areas.India has a rich heritage of indigenous fruit types. Significant advances have been made in recent times towards providing technology support for their cultivation.

Parasitology is the branch of biological sciences that deals with the study of parasites and all the related aspects. This leads to a basic question who is a parasite ? In fact, this term is frequently used in our society for those persons who are doing nothing and depending on others for their survival. The term Parasite means the organism (parasite) which depends on other organism (host) for its nutritional requirements. By definition, it excludes the organisms which are free living and do not depend on other organism for their food requirements. There are other details in fulfilling its nutritional requirements and important one is that in doing so, it causes some harm to the host other wise it will not be a parasite but a commensal.

Microorganisms are diverse group of organisms differing in many ways and range from saprophytic to virulent one. Virulent are pathogenic and harmful to human and animals and can cause latent to per acute disease. Nowadays although treatment is available for most of the diseases, it is better to prevent the disease rather than going for treatment. Development of immunity is the aim of immunization and hence through knowledge of the host parasite interaction, epitope of the microorganisms responsible for the inducing protective immune responses is of pivotal importance.

Year    Contributor    Description
1756    Duharmel du Monceau H.L.    Discovered callus formation in decorticated elm tree. 
1839    Schwann, T.H.    Proposed the view that each living cell of a multicellular organism would be capable of developing independently if provided with proper external conditions.
1853    Trecul, A.    Performed experiment on callus formation by decorticated trees such as Robinia, Pawlonia and Ulmus.

Climate change has become real and tangible affecting people. It is a major challenge for agriculture, food security and rural livelihoods. Climate change impacts on vulnerable groups have social, economic, political, and environmental implications. The impacts of climate change are global. India is more vulnerable in view of the high population depending on agriculture, which in turn is dependent on the seasonal monsoons. India is confronted with the challenge of sustaining rapid economic growth amidst the increasing global threat of climate change. Government of India has accorded top priority for addressing climate change related concerns.

Lima beans are flat green or light green beans. It is only utilised for the seed because the pods are coarse or woody. There are two kinds used in commercial canning: a climbing or pole variant and a dwarf or bush variation. Like other legumes used for seed, the lima bean (Phaseolus Iunatus) has a wide range in seed size, shape, and colour, as well as plant structure and primary applications. For freezing, canning, or fresh market usage, mature seeds are utilised like a vegetable. Lima’s have long been used as a grain legume when they are firm and dry. Peru is where they were discovered. The first domestication occurred in the Andes around 2000 BC, resulting in a large-seeded variety (Lima type), while the second occurred around 800 A.D. in Mesoamerica, resulting in a small-seeded variation (Sieva type).

The Species Concept in Systematics

The modern biological or, more strictly, genetical concept of a species is a group of interbreeding organisms, genetically isolated from other such groups. The definition of the category species has long been one of the major problems of taxonomy. It is because of two reasons:

1. Because of the congruity of lingering typological concepts with the nature of evolving species.

2. Because evolutionary species may have changing characters and are only gradually differentiated in phylogeny.

For evolutionary study and descriptive purposes, many kinds of variation within species come in to consideration, but the only regular intra specific taxon is the subspecies. Subspecies are widely but not universally useful and their definitions yield to the same sort of criteria as for dividing other phylogentic continuance.

Actinobacillus species are non-motile, Gram-negative rods (0.3 to 0.5 x 0.6 to 1.4 μm) which occasionally have a coccobacillary appearance. They are non-motile, non-sporeformingand non-acid-fast. A surface slime is presentin the three major species (A. ligllieresii. A. equuli andA. suis) and may be related to the stickiness of theircolonies on agar. Surface cultures have low viabilityand die in 5-7 days. The actinobacilli ferment carbohydrates, without the production of gas, within 24 hours and most species produce urease and grow on MacConkey agar. Most species are urease and oxidase positive. The
reactions in the oxidase andcatalase tests are variable. The genus is still in a stateof flux, mainly because of the close similaritiesbetween actinobacilli and species in the genera Pasteurella and Haemophilus.
These facultative anaerobes ferment carbohydrates producing acid but not gas. Actinobacilli exhibit some host specificity and are mainly pathogens of farm animals.
Usual Habitat
Actinobacilli are commensals on mucous membranes of animals particularly in the upper respiratory tract and oral cavity. As actinobacilli cannot survive for long in the environment, carrier animals play a major role in transmission.
Colonial Appearance
• A. lignieresii: small, glistening colonies develop in 24 hours. They are usually slightly sticky (viscid) on primary isolation but lose th is characteristic on subculture. The colonies are non-haemolytic and develop to about 2 mm in diameter in 48 hours. The organism grows well on MacConkey agar, the colonies are at first pale but become pinkish as A. lignieresii is a late lactose-fermemer.
• A. equuli: some strains are haemolytic and the colonies are sticky with this feature remaining on subculture. I t is a lactose-fermenter onMacConkey agar.

These include mainly tomato pomace, jack fruit waste, pineapple waste, apple pomace and citrus fruit processing waste.
1. Tomato pomace (Lycopersicon esculentum): Tomato pomace consists of the remnants of tomato processing, including peels, crushed seeds, and minimal pulp, which are left after extracting juice, paste, or ketchup. Following juice extraction, the pomace is typically dried and ground. Due to its high moisture content, artificial drying methods can be costly, making sun drying a more favorable option. The pomace should be dried until it reaches a crisp texture. After drying, it is essential to grind the pomace and incorporate the resulting product thoroughly into the diet. The composition of tomato pomace includes 93.5% DM, 17-22% CP, 39% CF, 11.1% EE, 0.44% Ca and 0.36% P, with 55% TDN. In terms of dietary inclusion, tomato pomace can be incorporated into the rations of adult bullocks and milch cows at levels of up to 50% and 16%, respectively, without negative effects. In dairy cows, the intake of dry matter, milk yield and milk composition remained unaffected. Additionally, fresh tomato pomace can serve as a supplementary feed for growing and finishing pigs. Notably, feeding growing pigs a diet containing 6% fresh tomato pomace resulted in a significant increase in feed consumption compared to those receiving a standard commercial mash.
2. Citrus by-products: Citrus fruits encompass several significant varieties, with the most notable being the orange (Citrus sinensis), lemon (Citrus limon) and grapefruit (Citrus paradisi). The by-products derived from citrus processing are affected by various factors, including the fruit’s origin and the method of processing employed. Consequently, the nutritional value of these by-products varies based on the type of waste and the specific fruit involved. The citrus by-products contain 1.7-7% TA, 1.2-2.1% EE, 5.7-8.6% CF, 2.2-4.2% CP, 65-75% NFE,
10.2-16.5% total sugar, 1.3-6.5% lignin and 4.4-12.8% pectin. Fresh citrus pulp waste is considered palatable for cattle, with a consumption capacity of up to 10 kg per day. Dried citrus pulp can be incorporated into animal feed at levels as high as 45% of the total ration. However, it is important to note that the digestibility of this feed in sheep diminishes when the inclusion exceeds 30%.

Introduction
Good Laboratory Practice (GLP) has been a cornerstone of laboratory operations, ensuring quality, reliability, and reproducibility in research and development across various fields, including pharmaceuticals, biotechnology, and environmental science. As we move further into the 21st century, the landscape of GLP is evolving rapidly, influenced by technological advancements, regulatory changes, and the increasing complexity of scientific research. This article explores future trends and innovations in GLP, delving into key areas such as automation, data management, regulatory shifts, and sustainable practices.
1. The Role of Automation in GLP
1.1 Increased Efficiency and Accuracy
One of the most significant trends in GLP is the integration of automation. Laboratory automation includes the prompt use of technology to perform tasks in a much efficient way with minimal human interventions, thus significantly increasing efficiency and accuracy of laboratory practices. Automated systems can handle repetitive tasks such as liquid handling, sample preparation, and data analysis, reducing the potential for human error.
Benefits of Automation
• Reduction of Human Error: Automation minimizes the chances of mistakes that can occur when humans perform these repetitive tasks, thereby improving the reliability of the results.
• Speed up the Research Process: For example, robotic systems can be used in drug discovery. These systems can conduct high-throughput screening, which means they can quickly test thousands of different compounds for potential effectiveness as drugs.