eChapters

Abstract

The studies were conducted during the years 2002 and 2003, to standardize the best method and time for summer budding in walnut. Four methods of summer budding viz. chip, annular, patch and shield were performed at 10 days interval from 15th May to 24th July. The results revealed that among the four methods of summer budding, chip budding was found to be highly superior to other methods of budding and gave 92% bud take success on 25th May and 87 per cent on 15th May also. Annular budding performed on 14th June was found next superior method and time of budding for walnut propagation when it resulted in 81% bud take success. Chip budded plants attained maximum height and radial growth as compared to annular and patch budded plants. The period from mid of May to first week of June was considered as optimum time for chip budding and mid June to end of June for annular and patch budding for walnut propagation under mid hill conditions of North-western Himalayas.

Abstract

In the present study, effect of phloroglucinol on the rooting behaviour of three Pyrus rootstocks viz. wild pear [P. pyrifolia (Burm F.) Nakai], kainth (P. pashia Buch. Ham.), shiara (P. serotina  Rehd.) and two scion cultivars viz. Patharnakh (P. pyrifolia (Burm F.) Nakai) and Punjab Beauty (P. pyrifolia x P. communis?) was studied. Supplementation of rooting medium (solid or liquid) with phloroglucinol (PG) had a stimulatory effect on the rooting response of wild pear, but on liquid medium the plants were vitrified with very small roots.  However, in solid medium fortified with NAA + IBA (0.25 mg l-1 each) and PG (160 mg l-1), 86.29 per cent rooting was obtained.  In kainth, Punjab Beauty and shiara supplementation of solid medium with PG had inhibitory effect on root induction.  In liquid medium, PG stimulated root initiation in kainth at 40 and 80 mg l-1, but at 160 mg l-1, it was ineffective.  In Punjab Beauty and shaira, liquid medium fortified with 40 mg l-1 PG was ineffective, whereas, at higher levels (80 and 160 mg l-1), it inhibited root induction.  Patharnakh failed to root at any of the PG level. One week culture of kainth microshoots on ½ MS solid medium supplemented with IBA (0.5 mg l-1) and PG (160 mg l-1) in dark followed by transfer to ½ MS basal liquid medium for three weeks in light resulted in 76 per cent rooting.  While, 58.70 per cent rooting was obtained in Patharnakh with similar treatment, except when IBA (0.5 mg l-1) was replaced by NAA (0.5 mg l-1).

Abstract

Propagation in vitro using shoot tips / axillary buds has been applied successfully for production of  clonal apple rootstock  Malling 7 of EMLA series. For surface sterilization, HgCl₂ was found to be a good disinfectant as compared to sodium hypochlorite. Shoot tips were established best in MS medium supplemented with 2 mg/l  BA, 0.5 mg/l GA₃ and  0.1 mg/l IBA. Antioxidants were added to overcome phenol exudation. Shoot multiplication was influenced by cytokinin type and concentration. Of the cytokinins tested, BA (0.5 mg/l) was found superior to kinetin. Addition of phloroglucinol improved multiplication rate and shoot growth. Among the auxins tested, IBA was found better and roots were readily induced with 1 mg/l IBA. 90-95 per cent plantlets were established successfully in peat containing pots as well as in the field.

Abstract

Old walnut seedlings in the age of up to twenty years were top worked using chip budding technique. In the seedling plants up to ten years of age, budding was done on older branches. But in the seedlings between 10-20 years of age, main branches were first dehorned during winters and the budding was performed on newly emerged branches in the month of May and June. Best results in the seedlings up to 10 years of age was obtained when budding was done in the second week of June with 89 and 97 per cent success in the year 2002 and 2003, respectively. The lowest success, however, was recorded in the plants between 10-20 years of age when budding was performed in the third week of May. Budding success in the plants between 10-20 years of age was slightly lower but they grew very fast as compared to those below ten years of age. Therefore, chip budding can be successfully utilized for the top working of old walnut seeding plants during second week of June when the buds become fully developed. For other areas this date may be different depending upon the maturity of the buds.

Abstract

Apple plantations in Himachal Pradesh have been ravaged by a unique hitherto unknown problem of premature leaf fall for the last one decade. In this study, the disease survey revealed 21.3 per cent disease incidence of premature leaf fall in July, 2001 which increased to 63.6 per cent by the month of September. Whereas in 2002, this disease appeared in August and the disease severity was 30 per cent in the September month. The pathogenic fungus Marssonina coronaria was isolated in pure culture and its pathogenicity was proved. Considerable number of conidia were formed in over wintered apple leaf litter with 22.7 per cent germinability. Ascosporic stage Diplocarpon mali was also encountered in the infected over-wintered leaves collected from Charna locality in Sirmour district. The sour tasting pollinizer cultivars Rus Pippin, Granny Smith, Tydeman’s Early Worcestor and King Pippin were rated tolerant in the field. Protective sprays of mancozeb, propineb, carbendazim, thiophanate methyl etc. were effective in controlling the disease. An integrated spray schedule was recommended to the growers.

Abstract

Wilt of gladiolus caused by Fusarium oxysporum f.sp. gladioli is a serious disease in Himachal Pradesh and disease incidence ranged between 19.0 to 50.5 at different gladiolus growing areas of the state during 2002 and 2003. Soil solarization of the infected field by covering it with transparent  polyethylene mulch (25 µm) for 40 days in summer months (March-April) was found effective to control the pathogen in soil.  Soil solarization resulted in average maximum temperature of 39.9oC in 2002 and 41.4oC in 2003 with an increase of 11.9 and 9.8^ºC, respectively in comparison to unsolarized soil. Increase in the average maximum soil temperature was found lethal to the Fusarium wilt pathogen of gladiolus. Soil solarization for 40 days resulted in significant reduction (84.6 %) in the colony forming units of the pathogen at 5 cm soil depth in comparison to unsolarized control. Soil solarization also resulted in 63 per cent reduction in disease incidence, recorded increase in different growth parameter of crop and also resulted in early flowering of the spikes.

Abstract

Powdery mildew infected leaves of apple hyperparasitized by Ampelomyces quisqualis were collected from field and examined for the presence of the dark brown pycnidia of  hyperparasite. The hyperparasite was isolated on potato dextrose agar medium by standard isolation technique. The morphological characters of the hyperparasite such as size and shape of pycnidia and pycnidiospores were studied. Six systemic fungicides were evaluated for their compatibility with A. quisqualis under in vitro conditions. Dinocap was least inhibitory giving 65.56 per cent growth inhibition of A. quisqualis followed by mancozeb (66.67%). Effect of these fungicides was also seen on conidial germination and germ tube length of A. quisqualis. Maximum conidial germination and germ tube length was observed in mancozeb followed by benomyl and dinocap.

Abstract

Apple orchardist’s are adopting micro irrigation system for irrigation and application of  fertilizers. The feasibility of delivery of pesticides through micro irrigation for the management of diseases and insect pests was evaluated. The minimum root rot incidence (30.55%) was recorded at low fertigation dose + aureofungin (100ppm)+ copper sulphate (100ppm) at two-week intervals as compared to control (61.10%) and it also delayed the symptom appearance by one month. At higher fertigation dose + aureofungin (100ppm) + copper sulphate (100ppm) there was increase in disease incidence as compared to the control (without fertigation). Continuous maintenance of soil moisture at field capacity (FC) enhanced the disease incidence where the overall disease incidence was 63.88 per cent as compared to 27.77 per cent in irrigation at 40 per cent FC. Fungicides applied at bi-weekly interval recorded less disease incidence. The fertigation levels did not affect powdery mildew severity; however, irrigation levels influenced it.  Plants maintained at 40 per cent FC showed less disease severity as compared to those maintained at 100 and 60 per cent FC.  Plants receiving hexaconazole dose at weekly interval depicted less disease than 2 or 4-week interval. The number of colonies and population of woolly aphid were  less on the plants treated with chlorpyriphos.

Abstract

Amongst various soil-borne diseases of apple, white root rot (Dematophora necatrix) causes severe losses both in nursery as well as in grown up orchards and is a limiting factor in raising healthy nursery of apple. Seedlings are killed within a short period depending upon the age of the host and environmental factors. The present investigations were, therefore, undertaken to study the population dynamics of VAM fungi in rhizosphere soil  of apple plant alone and in combination with green amendments and its role in disease suppression. The study revealed that there was variation in population dynamics of VAM fungi alone and in combination with green amendments. It was observed that apple seedlings grown in VAM inoculated fungi (Glomus sp. and Glomus heterosporum) and green amended   soils had more VAM population and colonization in roots than non-amended (minus crop residue) soils after pathogen inoculation. The population of Glomus sp.(350/100gm soil) was maximum in soybean+ Glomus sp. inoculated soil and it was observed that VAM fungi and crop residues in combination suppresses the disease to maximum extent as compared to their alone effect and only 8.7 per cent disease index was recorded in apple seedlings inoculated with mungbean+ Glomus sp. followed by 16.03% in soybean+ Glomus sp. inoculated seedlings.

Abstract

Twenty seven genotypes including two wild relatives of strawberry were evaluated for resistance against leaf spot caused by various fungal species viz.,Phyllosticta, Pestalotia, Hainesia lythri and Phyllosticta fragaricola under natural conditions. None of the genotypes was found to be resistant against leaf spot. However, the cultivar ‘Fern’ showed moderately susceptible reaction whereas the remaining genotypes were either susceptible or highly susceptible, suggesting the need for diversifying the germplasm either through open pollinated seedling selection or through hybridizing genotypes of divergent origin for resistance against leaf spot.

Abstract

During inspection of several lily plantations under cover in different districts of Himachal Pradesh plants with severe disease symptoms were observed. The affected plants showed a progressive loss of vigor, were stunted and had a severely malformed leaves. These symptoms were observed on large scale mainly in oriental lily hybrids ‘Acapulaco’. The plants were suspected to be infected with phytoplasma, the presence of which was demonstrated with fluorescent microscopy (DAPI stain). The phytoplasma etiology was further confirmed by conducting nested polymerase chain reaction (PCR) assays using universal primer pairs P1/P7 followed by R16F2n/R16R2, which amplified a fragment of phytoplasma 16SrDNA. This is the first report of phytoplasma infection in lilies identified by molecular assays from India.

Abstract

Survey conducted in different Lilium growing areas of Himachal Pradesh revealed the incidence of virus diseases between 15 and 95 per cent in different localities. Mild mottling, curling and necrosis of leaves, deformation and virescence of floral bulbs and flowers and reduction in bulb size were the most prominent symptoms observed. The virus isolate was transmissible through sap, bulb and aphid vectors and had a narrow host range. Electron microscopic studies revealed the presence of filamentous flexuous virus particles measuring 640×17-18nm in size.

Abstract

On the basis of symptoms, samples from seven localities within Solan district (H.P.) at varying altitudes ranging from 600-1650mts comprising Nalagarh (600-750m), Badghal (850m), Dilchab (950-1000m), Shaya (1200m), Solan (1300-1450m), Bajhyana (1520m) and Tikari Pajahali (1650m), were collected and used for serological detection by DAS ELISA. It was found that all the isolates except Nalagarh, reacted positively with the antisera to ToLCV, thus substantiating the results on the identity of the present isolates. Out of the seven isolates under study, Dilchab and Shaya isolates did not show any PCR amplification for Rep gene indicating the variability in the regions flanking the Rep gene. Likewise, no amplification result in case of Nalagarh sample proposes the attack of some other pathogen producing similar symptoms.

Abstract

Population dynamics studies of phytoparasitic nematodes (mainly 2nd stage juveniles of root-knot nematode; Meloidogyne incognita) in the rhizosphere of persimmon (Diospyros kaki ) orchard, were carried out, in relation to seasonal fluctuations. There was recorded remarkable impact of temperature on the nematode population.  Highest nematode populations (7148/ 200 cc soil) were recorded during May, when temperature was maximum; 22.9^ºC (15.7- 30.0^ºC). Least population density of the nematode were extracted during January when temperature remained minimum; 7.9^ºC (2.4-13.3^ºC). However, relative humidity during entire course of investigation did not show any considerable impact on the population fluctuation of the nematode. With the fall in temperature from July onwards up to January (22.6- 7.9 ^ºC) there was gradual decline in the nematode population (3813- 468/ 200 cc soil), showing thereby that nematode populations in the rhizosphere is directly affected by the prevailing temperature and thereby soil temperature.

Abstract

Ten pests belonging to six insect and mite orders were recorded infesting strawberry cv. Chandler in Paonta and Doon Valleys of North Indian during 2001-04. Out of these Heliothis armigera Hubner, Spodoptera litura F., Thrips tabaci  Kheg, Holotrichia insularis Brenke, Odontotermes obesus Ramb and Tetranychus telarius L were of primary significance. Two fortnightly application of endosulfan (0.05%) from mid April proved effective in containing the attack of first two most devastating pests.

Abstract

Among different acaricides evaluated, propargite (0.0285%) provided maximum control (98.6%) followed by fenzaquin (0.0025%) with 97.2 per cent control. Both propargite and fenzaquin were effective even 14 days after spray giving 71.0 to 80.6 per cent control. Horticultural mineral oils (1.0 %) were also effective and provided 87.7 to 93.8 per cent mortality of the pest after 24 hours of spray, however, their efficacy declined after 14 days of spray to 43.2 to 60.1 per cent. It was concluded that propargite (0.0285 % and 0.057%) and fenzaquin (0.001% and 0.0025%) provided effective control beyond 15 days of spray. Horticultural mineral oil treated plants, however, needed to be retreated in about 15 days.

Abstract

European red mite is a  serious pest of apple in Himachal Pradesh. Recent survey has shown that 80 per cent apple orchards are infested with this pest. As a result of continuous feeding fruit remained undersized, poor coloured and sour in taste. New acaricides fenazaquin (0.001%) and propergite (0.057%) proved highly effective Standard acaricides dicofol ( 0.037%) and insecticide endosulfan (0.07%) remained effective upto 15 days only.

Abstract

Cut flowers of rose cv. ‘First Red’, with uniform stem length of 45 cm, harvested during different times of the year i.e. March, May and October, were used for standardization of holding solutions to increase the vase life. Harvest-wise pooled averages of two years data (2003-04) revealed that the cut flowers of March and May harvests gave increased flower diameter, more volume of solution consumed, maximum vase-life, reducing sugar and protein content with minimum weight loss when these were held in a solution containing 100 ppm 8-HQC (8-hydroxy quinoline citrate) +1.5 per cent sucrose. For getting similar results of October harvested flowers lower concentration of 8-HQC (50 ppm) with same level of sucrose was found most suitable

Abstract

The effects of packaging materials, temperature and dressers on bell pepper (Capsicum annuum, L.) seed storage, at the end of 24 months, revealed maximum test weight (5.15 g) in poly jar x refrigerator x cow dung. Maximum seed germination was observed in polythene bag x refrigerator x thiram. This combination resulted in slow but gradual decrease in germination from 3rd month (95.33%) to 24th month (75.33%). The same combination of treatments also extrapolated maximum SV-I (15-24 months) and  (Seed Vigour)SV-II (18-24 months). Stress test was maximum (50.01%) in poly jar x refrigerator x thiram. Thus, it was inferred that bell-pepper seed germination and vigour can be maintained for 24 months after treating the seeds with thiram, packing in polythene bag and then storing in refrigerator, after harvesting the crop.

Abstract

The effects of packaging materials, temperature and dressers on onion (Allium cepa, L.) seed storage, at the end of 24 months, revealed maximum test weight (3.67 g) in poly jar x refrigerator x cow dung. Maximum seed germination was observed in cloth bag x refrigerator x thiram. Maximum germination of 87.33 per cent at the end of 21 months was recorded in poly bag x room temperature x thiram which declind to 77.33 per cent by the end of 24 months. Similarly (seed vigour) SV-I, SV-II (up to 21 months) and stress test (up to 18 months) were maximum which showed a declining trend after this period. It can be inferred that the onion seed germination and vigour can be maintained for 21 months when packed in poly jar, stored in deep freezer (-20°C) after treating with thiram (0.3%).

Abstract

A considerable difference of gap has been observed between the generated technology and the technology available with the farmers. In order to find the possible causes or low production, a comprehensive bench mark survey of four Panchayats of Kandaghat block namely Sirinagar, Mahi, Kawarag and Bisha involving 500 farm families was done using PRA technique to ascertain the extent and gap in the generated technology and the technology adopted by the farmers. All the important components of the technology were assessed and the gap in adoption as well as the approximate cost of the gaps was estimated. In tomato and capsicum, it was observed that the farmers prepared their fields well, transplanted at the right time, performed the required intercultural operations and harvested the fruits at proper time. They however, did not treat the seed and the seed bed, applied sub-optimal/excess doses of fertilizes and adopted excessive plant protection measures against pests and diseases. In case of Cauliflower, the farmers used the recommended variety, prepared their fields well, transplanted at optimum time, adopted all cultural operations from time to time but they did not pay attention for protecting the crop against pest and diseases.They also applied ample quantity of fertilizers but did not follow proper spacing. If the farmers adopt the recommended practices, they are likely to gain around Rs 62,688/ha in tomato, Rs.55,563/ha in capsicum and Rs.54,188/ha in cauliflower.

Abstract

Temperate fruit production in India is mainly confined to the Himalayan region particularly in north-west states namely J&K, HP and Uttaranchal and to the east in Arunachal Pradesh.Though these areas are sparsely populated, yet are the main centers for production of these fruits. Apple is the leading cash fruit among these fruits occupying nearly 85.0 per cent of the total area 90 per cent production under these temperate fruits.

The apple cultivation in these states is more than 100 years old where the English settlers planted acidic types varieties in Kullu, Shimla, Chaubattia and Kodikonal hills. Quality Delicious apples which were introduced by an American Missionary Samuel N.Sokes in 1917 at Kotgarh, form the today’s commercial apple production in H.P. exception Kashmir valley, temperate fruit production in India is in temperate type of climate available in these states due to higher hills experiencing adequate winter cold and snowfall.

Horticulture in the hills has special significance and specific requirements to sustain it for the improvement of the lot of the people living in the hills. The national policy on horticulture has to be so oriented so that it recognizes the varied agro climatic and soil conditions. The dynamic process of development and extension has to be specifically designed to cater to the requirements of the hill states; which, because of the difficult terrain, are by and large under-developed as compared to the rest of the country. The new national policy on horticulture should take into account the following points in order to make temperate horticulture a self sustaining enterprise.

Everyone on this earth wishes to have a healthy life free from all ailments, stress and strain. But it is a common observation that some of us are more susceptible to diseases and fall victim of many infections while others have the capacity to resist many such infections. They are not so much susceptible to diseases. Is there anything which distinguishes them from others? An organism is made up of organs and organs systems. Like many other organ systems, in the body there is a system which is concerned with the protection of the body from infections.

Like any other organ system in the body, immune system comprises of various organs and cells. The major component of the immune system is the lymphocytes which constitute lymphoid system. The lymphoid organs are classified into two categories: primary and secondary lymphoid organs.

Primary lymphoid organs: These are the organs where B and T lymphocytes undergo maturation. Bone marrow and thymus are the primary lymphoid organs. These are the organs where lymphocytes differentiate, proliferate, get selected and mature into functional cells.

The main purpose of the immune system is to protect the body from foreign substances, diseases or infections. When the body is exposed to a microbe or a foreign substance (antigen), the body makes some response which is called immune response. The purpose of immune response is to recognize, neutralize and eliminate the antigens from the body. Generally, the term immunogen is also used for antigen. Both, antigen and immunogen are used as synonyms but specifically, they are different from each other.

Antibodies are a group of glycoproteins having a globular compact structure. They are known as immunoglobulins. On the basis of their experiments, Tiselius and Kabat, in 1939, demonstrated that most antibodies are found in the g globulin fraction of serum proteins. When serum from rabbit, immunized with ova albumin, was subjected to electrophoresis, it showed a peak for g globulin (Fig. 4.1). Studies have revealed that the major part of IgG is found in the g globulin fraction of the serum proteins. Other fractions, a and b, too, have other immunoglobulins along with small part of IgG.

Antigen-antibody interaction is very specific one in which an antibody recognizes an antigen through complementarities of shapes on paratope and epitope.  It is like lock and key, as occurs in case of enzyme-substrate reaction. It does not involve covalent linkage rather a spatial complementarity occurs between antigen and antibody. For example, when antibodies, raised against m-amino benzene sulphonate, are tested for their ability to bind with ortho-, meta- and para-isomers of the hapten, the antibodies show highest strength with meta than ortho- isomer.  Antibodies show poor reactivity with para isomer. This is because though chemically all these molecules are same but structurally they are different.

All blood cells arise from a basic cell called the hematopoietic stem cell. Stem cells are undifferentiated embryonic cells.They are self renewing and their population by cell division. Stem cells have the ability to develop into any type of blood cell. The formation of blood cells begins in the embryonic yolk sac during the first week of development. Every mature cell is derived from the same type of stem cell. In contrast to an unipotent cell which differentiates into a single cell type, a hematopoietic stem cell is multipotent or pluripotent. In the mammalian embryo, the blood corpuscles are normally formed in yolk sac, liver, spleen and thymus gland.

The immune system protects the body from harmful substances by recognizing and responding to antigens. Antigens are the protein molecules which are present on bacteria, virus, fungi or some other microorganisms.Some toxins can also be antigens. These antigens are recognized by immune system. The destruction of antigens by producing antibody is called antibody-mediated immune response. As antibodies are present in the body fluids i.e. humors,this type of response is called humoral immune response.

Cell mediated immune response performs different functions in protecting the immune system. When antigens are destroyed by cells, without producing antibodies, the immunity is called cell-mediated immunity (CMI) or cellular immunity. In this immunity T cells play a major role. The principal role of cell mediated immunity is to detect and eliminate the cells that have intracellular pathogens. When the antigen remains inside the cell the antibody cannot handle them. The intracellular pathogens are controlled by cell- mediated immunity. CMI is invited by the recognition of antigens by T cells. T cell lymphocytes recognize protein antigens of intracellular microbes that are displayed on the cell surface of infected cells. It is also important for elimination of cells which express foreign MHC molecules. Antigen specific and nonspecific cells can contribute to the cell-mediated immune response. The activities of both specific and non-specific components usually depend on effective local concentration of cytokines.

The complement system is a group of about 20 heat labile plasma proteins which constitute nearly 10% of total serum proteins. These are important in activation of phagocytes, control of inflammation and cytolysis. They form one of the major immune defense systems of the body. The complement system plays important role both in innate and adaptive immune system. The main functions of complement are chemotaxis; opsonization and cell activation; immune clearance of immune complexes from circulation; lysis of target cells; triggering and amplification of inflammatory reactions and development of antibody responses. Evolutionary, the system is very ancient and is present even in invertebrate organisms like starfish and worms.

Major Histocompatibility Complex (MHC) is a closely linked cluster of genes which have codes for cell surface proteins known as  MHC molecules. These molecules have very important role in presentation of processed antigens to the T cells in initiation of an immune response as T cells recognize an antigen only when it is presented along with MHC molecule. These are highly polymorphic and are the major barrier to organ/tissue transplantation. All vertebrates have MHC. MHC differs among members of a species and are thus called alloantigens. MHC participates in the development of both humoral and cell-mediated immune responses.

Cytokines are the chemical messengers that are involved in interactions between the cells of innate and adaptive immune responses. They are secreted by leukocytes and many other cells in the body. They are low molecular weight proteins or glycoproteins which are produced in response to various kinds of stimuli. Cytokines have important role in immune and inflammatory responses.  Their functions are not limited to immune system only.They also stimulate the development of hematopoietic cells and also act as therapeutic agents.

The immune system has been designed to provide protection to the body from various diseases. But the same system which is aimed to provide protection may sometimes become harmful to the body and may even life-threatening. There are situations when immune response against harmless and inert antigens is exaggerated and actually causes diseases. Such a condition is called hypersensitivity or commonly as allergy. Though the term ‘hypersensitivity’ indicates an increased response but the immune response is not always heightened, instead it may be an inappropriate response to an antigen.

Infection refers to the invasion of harmful microorganisms into the body and causing disease. The most important function of the immune system is to protect the body of the host against various viruses, bacteria, fungi and parasites. The grounds of immune surveillance stand on the basis of innate and specific immunity that build up against the evasive mechanisms of the disease causing organisms. Infectious disease is result of a complex interaction between  the host and the microbes. Evasions of protective immunity by the parasites critically determine the survival and pathogenicity of the microbes. The invasion of the body by harmful microorganisms is called infection. Infections are dangerous in any part of the body. Microorganisms spread vary easily into the blood stream and cause blood poisoning which is called septicemia.

One of the greatest achievements in science is the development of vaccines. Vaccines have been revolutionary for the prevention of infectious diseases. They have prevented a large number of deaths caused, every year, by dreadful diseases like smallpox, polio, hepatitis etc. Diseases like smallpox and polio have been successfully eradicated by vaccination. Normally, when the body encounters a microbe, both humoral and cytotoxic immune responses are induced to neutralize its effect. In addition to eliminate the invader, body forms memory cells which help the body to protect itself from the same pathogen, in future. Vaccines not only function to kill the pathogens but they also stimulate the body to form memory cells which provide immunity against a specific pathogen

Immunity is defined as the resistance to infection. This is carried out by the process of identification and removal of non-self material. In contrast to this, our body has to maintain immune tolerance to our own cells and tissues and develop the ability to distinguish these self-antigensfrom non-self (foreign) antigens. Tolerance means unresponsiveness of the immune system to an antigen. Immune tolerance is the process by which the immune system does not destroy an antigen. Tolerance can be either natural or induced.

Transplantation refers to the implantation of a tissue or organ from one site  to another site in an individual or from one individual to another.It involves implanting of organs or grafting of tissues to repair defects and to stimulate regeneration. Auto-transplantation or autoplasty of the skin, cartilage, bone, muscle, tendon, nerve and fatty tissue is used in plastic and cosmetic surgery. The replacement of a diseased organ by a transplant of a healthy tissue is called transplantation.

A tumor is said to be an abnormal growth of cells to form a lump or mass of transformed cells. This growth occurs due to loss of regulatory control of cell division. The majority of cells and organs have a balance between cell renewal and cell death. A cell has a particular life span;when it dies a new cell is generated. In normal condition, this system is regulated through cell cycle and cell division but at the time of any abnormality this growth mechanism is totally disturbed and failed. These cells give rise to clone of the cells and eventually produce tumor.

The well-being of a person depends on his immune system. Any deficiency in any component of immune system may lead to defects in the normal functioning of his body and makes him more susceptible to diseases. The immunodeficiency may be either in specific components (B cells or T cells) or nonspecific component (phagocytes and complement) of the immune system.

One of the characteristics of the immune system is to recognize and discriminate self from the non-self antigens.  But, sometimes, there is a failure to discriminate between self and non-self antigens in the body and an autoimmune response occurs. Body starts producing antibodies against its own cells/antigens. These antibodies are called autoantibodies and this state is called autoimmunity.

Many techniques are used in immunology to detect specific proteins (antigens) or antibodies in biological samples. These techniques are called immunotechniques. These methods are used for research and diagnostic purpose in many fields such as microbiology, biochemistry, endocrinology, hematology and in pathological laboratories. The techniques can be used to estimate proteins, drugs, hormones and toxic substances in a sample. Immunological techniques are highly specific, precise and reliable. These methods are simple and safe. Antigen and antibody reaction is highly specific in nature and this forms the base of the immunological methods. There are many immunotechniques which are used for the qualitative and quantitative estimation of antigens and antibodies in a sample. Some of commonly used techniques in immunology are:

Animal protein has a great importance in human health by providing essential amino acids. It is supplied by the milk, meat, fish, eggs etc. Specifically meat has a prime position in the human’s diet particularly for those who are non- vegetarians. As per Indian Council of Medical Research (ICMR) recommendations a normal human being requires 60 gram protein in a day with the net protein utilization (NPU) of 65. In ideal cases 30 to 50 percent of daily protein intake should be from animal sources to get optimal range of amino acids. In present scenario human beings all over the globe are getting 29 gram of protein while developing countries have 15 g per day availability and developed nations are getting 60 grams protein per day per head. However, in India availability of protein is only 10 grams/day/head.

To streamline the slaughter activities throughout country and to produce the quality meat for domestic as well export market, Bureau of Indian Standards (BIS) formulated certain standards related to abattoir and other food items. It also harmonizes the standards as per the requirement of importing country. So that traders can easily meet out the demand of importing country. Under the standard provisions of BIS, each and every aspect of abattoir as well as its processing aspect is very well described in these standards. These standards are written and documented and easily available to know how about their implementations.

The aim of pre-slaughter care of animals is to arrange physiologically normal animals for slaughtering without loss of weight and to minimize stress to the animals. Pre-slaughter care of meat animals includes proper handling of animals during loading, unloading, transportation, lairage care and handling of animals just prior to slaughter. Thus due care must be adopted in this stage not only for welfare point of view but also for production of good quality meat. Proper care and handling is also essential to reduce the chances of bruises, cuts, tears and deformities on the animals/birds body.

Ante-mortem examination includes all procedure or tests conducted by a competent person on live animals for the purpose of judgement of safety, suitability and disposition. It is an integral step to be performed by qualified veterinarian during conversion of animal to carcass for wholesome meat production. The animals are examined for cruelty due to overcrowding or suffocation or any other inflicted injuries and stress during transportation or otherwise, because it not only directly affects the quality of meat but also downgrade the carcass. Ante-mortem inspection ensures that only healthy animals are slaughtered and wholesome meat reaches to the consumers. A complete record of the ante-mortem inspection of all animals received in the abattoir should be maintained to ascertain that animal’s health status at the time of slaughter. Antemortem examination not only ensures the health animals entry into slaughter house but also assist in postmortem examination. It is also important to make a decision on certain diseases based on symptoms because they may not show the postmortem lesions for final judgment. Ante-mortem inspection has twofold functions as it enables veterinarians to protect public health and to assess the welfare conditions in which the animal is placed.

Post-mortem examination is a thorough examination or inspection of all relevant parts of slaughtered animals including carcass and organs by qualified veterinarian immediately after slaughter and evisceration to find out the possible changes and lesions that indicate unsuitability of the meat for food. Postmortem examination should be carried out as soon as possible after carcass dressing otherwise carcass gets set and the examination of lymph nodes becomes difficult particularly in beef and pork in winters.

Slaughter is an act of killing or destruction of animals for food purpose and includes all processes and operations on all such animals in order to prepare it for being slaughtered. It is an expert act requires expertise and willingness. Slaughter is necessarily to be performed in a slaughter house recognized or licensed by authority empowered under law for the time being in force to do so.  Only authorized animals are to be slaughtered for food purpose and restricted animals or animals prohibited for slaughter should not be converted into meat in any case. Animals in pregnancy, has offspring less than three months or under the age of three months, animals not certified by veterinarian for fitness should never be slaughtered. Slaughterman should also have a valid license or authorization issued by municipal or other authority. The persons below eighteen years of age and suffering from communicable or infectious diseases shall not be permitted to slaughter an animal.

Humane slaughter is an act of killing the food animals in such a way as to cause the minimum stress or distress to the animal. To achieve this objective animal must be rendered insensible instantaneously and remain insensitive to pain until there is a complete loss of brain responsiveness due to exsanguination. The unconsciousness in animals is induced by stunning procedures which are varying in number. The method of stunning primarily based on species, breed, age, sex, kind and mental state of animals. Stunning is essential to safeguard the feelings and anxieties of animals. It facilitates maximum bleeding from the animals because animal becomes unconscious after stunning. To make animal unconscious before bleeding is not only essential for animal welfare point of view but it is legislation in many countries.