eChapters

6.1 Introduction

The food industry produces large volumes of waste both solid and liquid, starting from the initial step of raw material to the final consumption of processed food. Apart from a loss of valuable biomass and nutrients, the generated waste poses increased disposal and potentially severe pollution problems. Meat and meat industry is no exception where slaughter houses, meat processors, wholesalers and rendering plant generates huge amount of waste [1, 2].

Meat and meat processing industry, is among the least profitable industries despite its immense size and large gross sales. Effective recovery and utilization of by-products and wastes is the lifeline of meat and poultry processing industry. Thus, it becomes imperative that an effort be made to reduce expenses by employing the use of new or modified processing methods and through in-plant treatment, where waste effluents and by-products could be recovered and often upgraded to useful products of higher value. Meat processing wastes have a potential for recycling raw materials or for conversion into useful products of higher value as by-product or even as raw material for other industries or for use as food or feed after biological treatment.

1. AN OVERVIEW OF RWANDA’S SOCIO-ECONOMIC SETTING

Rwanda is a small landlocked country located in East Africa covering a total surface area of 26,338 km². It lies about 120 km south of the Equator and is bordered by Uganda in the North, Tanzania in the east, Burundi in the south and the Democratic Republic of Congo in the West. According to the national official statistics, the total population is approximately 8,200,000 people, with women accounting for 55 per cent of the people. The country’s population density is 505 people per km², making it one of the most densely populated countries in Africa (National Institute of Statistics, 2002). Since the turn of the millennium, the annual growth rate of the population has been 2.9 per cent and it is believed that by the year 2020, the country will have an estimated population of 20 million, thus exerting pressure on the limited land resources. It is also important to note that most people (64 per cent), those living in abject poverty, reside in rural areas of the country (Government of Rwanda, 2008).

Size – Shapes - Arrangements – Morphology

Bacteria are very small, most being approximately 0.5 to 1.0 mm diameter. Smallest bacteria are Mycoplasmas, as small as 0.2 micrometers (almost as small as largest poxviruses).

Accepted wisdom is that bacteria are smaller than eukaryotes. But certain cyanobacteria are quite large; Oscillatoria cells are 7 micrometers diameter, size of red blood cells. And certain eukaryotes (e.g. Nanochlorum eukaryotum) are very small, only 1 to 2 micrometers, but true eukaryotes (nucleus, chloroplast, mitochondrion are present). So size difference is, like many generalizations, only a useful yardstick, not an absolute truth.

Epulopiscium fishelsoni, discovered in 1985 in intestinal tract of sturgeonfish, is an enormous, cigar-shaped cell, as large as 80 x 600 micrometers (that’s 0.6 mm, large enough to be seen by the naked eye). Amazingly, this cell is prokaryotic! Initial evidence by EM was hard to believe, but confirmed rRNA comparisons with other organisms, a cousin of Gram-positive Clostridium genus.

The relative size of the bacteria are as follows :

11.1 Introduction

Phytosterols or plant sterols are bioactive plant constituents having similar chemical structure and biological functions as cholesterol (cholest-5-en-3b-ol), but differ in their side chain configuration. They are non-nutritive compounds with chemical structure differing from that of cholesterol only by minor modifications like in the structure of their side chain by a methyl or ethyl group at C-24. Sitosterol and campesterol have an ethyl and a methyl group at C-24, respectively (Fig. 11.1). Phytosterols present a tetracyclic ring and side chains linked to C-17 that differ according to sterol compound same as cholesterol, to which they resemble structurally as well as biosynthetically. Saturated form of sterols referred to as stands, occurring in small amounts, mainly in cereals are represented by the absence of the double bond at the D5 position. Phytosterols represent a part of the broad group of isoprenoides and are 28- and 29-carbon atom steroid alcohols naturally occurring in plants. Phytosterols and phytostanols are triterpene compounds, found ubiquitously in plants which can only be synthesized by plants, whereas humans and animals obtain them from their diet.

Definition

“Biological pesticides”, or “Biopesticides” as the name suggests, are naturally occurring substances that biologically control harmful pests, especially among field crops. These are naturally produced bio chemical materials basically non-toxic to the environment that can be employed in pest control. Biopesticides could mean living organisms (bacteria, virus, and algae), their products (biochemicals produced by them) and also plant byproducts. Biopesticides offer an ecologically effective solution to the harmful effects of synthetic pesticides.

ABSTRACT

Mushrooms are in use for centuries in traditional system of medicines, and have importance in greater Chinese and western pharmacopeia. Morchella species are well known across the globe as a popular and prized edible fungi and unique culinary saprophytes. They have a long history of usage in the formulation of different traditional medicine for centuries due to their anti- oxidant, anti-inflammatory, anti-cancerous and immuno-stimulatory properties. Inspite of high demand and day-by-day increasing importance, cultivation of M. esculenta Dill.ex Pers and other morels are limited, however, there is a report that some species of Morchella are successfully cultivated in China. Phytochemical studies reveals that polysaccharide groups, phenolic compounds, tocopherols, ascorbic acid and vitamin D are the most active molecules present in morels. In addition to these compounds, morels are also rich in carbohydrates, amino acids, fatty acids, organic acids and several kinds of macro-and micronutrients. Literature revealed that no data on reproductive biology of M. esculenta are available till date. Future investigation is required to study the life cycle and the ecological association of different morels to high altitude regions, and causes of their association with coniferous and broad leaved trees such as Cedrus deodara, Pinus gerardiana, Picea smithiana, Quercus and Castanopsis species. There is an urgent need to explore all traditional knowledge associated with morels, phytochemistrys and associated pharmological studies of major compounds, reproductive cycle and their basic biology for future conservation of this precious mushrooms for man-kind.

Abstract: Hollyhocks are seed propagated garden annuals with numerous hybrid phenotypes, series (single and bicoloured Halo series), Spotlight, Carter’s Double, Double Triumph, Giant Double Powder Puff, Halo Bush (tricoloured), Halo Mixed (eight colours) and cultivars are available in the world market. Unusual plant height, delayed flowering compared to other winter annuals handicap its popularity. While little genetic information and trait heritability has been established for this crop, breeding continues to produce new variants for commercial production. Gene pool of hollyhock exhibits great diversity. Breeding objectives with high priority include dwarf stature (2 feet height), compact growth, double and large flowered inflorescence, precocious flowering and resistance to leaf rust disease.

ABSTRACT

The investigation was carried out to develop protocol for micropropagation of acid lime (Citrus aurantifolia S) var. Sai-Sharbati. Shoot tip and nodal segment explants were obtained from axenically grown seedlings having 21 to 30 days age. Direct regeneration of shoots, roots and whole plant without intervention of callus was obtained from both the explants on MS basal media. In both the explants 100 per cent shoot proliferation was induced in nutrient medium MS + BA 0.25 mg/l + ME 200 mg/l. The shoot proliferation rate was more (5.0) in nodal segment as compare to the shoot tip explant (4.33). Regenerated shoots of more than 2.5 cm length with minimum of 4 leaves were transferred for rooting. Half strength MS medium supplemented with IBA 1.0 mg/l was best for root induction and root initiation within 15.66 days. More than 75 per cent plantlets were survived after hardening period of four weeks.

Introduction

R is one of the most popular platforms for data analysis and visualization currently available. It is free, open-source software, with versions for Windows, Mac OS X, and Linux operating systems. R is a language and environment for statistical computing and graphics, similar to the S language originally developed at Bell Labs. It’s an open source solution to data analysis that’s supported by a large and active worldwide research community. But there are many popular statistical and graphing packages available e.g. Microsoft Excel, SAS, IBM SPSS, Stata, and Minitab etc., however R has many interesting features so why it is mostly followed by researchers and academicians. Few of the major features of R are given below:

Agriculture is the most important sector in India in terms of the population dependent on it. Although agriculture now accounts for on 14 per cent of GDP, it is still main source of livelihood for the majority of the rural population. Rapid growth of agriculture is critical for development of rural economy and viable development of rural economy will leads to overall development of country. Diversification in agriculture is need of the hour, any cropping system comprising crops with varied maturity period, crop canopy and high yielding potential is need for an average farmer. Again cropping systems depending upon the soil type, total rainfall and arrival of monsoon in the region. Low cost technology is essential for reducing financial burden on farmers and to increase production on sustainable manner.

Anthracnose

Colletotrichum lindemuthianum, Glomerella lindemuthiana Shear [teleomorph]

Economic Importance

• Anthracnose is an important disease in Kenya, Uganda, Brazil, Argentina, Mexico, Guatemala, Costa Rica, Nicaragua, India, Peru, Ecuador, and Colombia .
• The disease causes huge losses in temperate and subtropical zones.
• Yield losses of 95% have been recorded in Colombia and over 92% in Malawi.
• Anthracnose also affects seed quality and marketability of the crop.

A new buzz-word is rapidly spreading through the manufacturing and analytical environment. Discussions on the application of Non Invasive Research namely the Near Infrared (NIR) Spectroscopy are often found on the agenda of production, project, and technical and even board meetings. Engineers and chemists are confronted more frequently with the challenge to decide whether a NIR instrument will be the solution to their production quality control and other analytical problems. Are there really amazing machines that can determine protein, fat, moisture, fibre, starch, nicotine, alcohol, sugar, amino acids and whatever analyses you require on any product from foodstuff to fertilizer and animal feeds in less than a minute and so simple to operate that even the unskilled labourer can handle it? In this article we will endeavor to answer the questions that often crop up in the evaluation of NIR as an analytical technique towards Natural Products.

Abstract

Pomegranate, Punica granatum L., is an ancient fruit tree native to the Middle East. All plant parts are rich source of the various secondary metabolites that accounts for its antioxidant and health-promoting activity. A strong genetic control on anthocyanin production is responsible for variability in the color of peel and arils of pomegranate. Characterization, cloning and expression of the genes related with anthocyanin and tannin production provides routes for engineering of these complex pathways. Recent studies on pomegranate genome sequencing have elucidated several biochemical pathways and candidate genes that could assist to increase production of potent bioactive compounds in near future. This will further facilitate our understanding to some exclusive biological processes that plays a significant role in pomegranate breeding.

Development of the face includes the development of orbital, nasal and oral regions. Development of face involves five facial primordia during the embryonic period of development. These primordia include the single frontonasal prominence and the paired maxillary and mandibular prominences.

The frontonasal prominence forms two sets of bilateral ectodermal thickenings, the nasal and lens placodes. Frontonasal prominence shows nasal component (of ridges) and a frontal component. A thickned area of ectoderm olfactory/ nasal placode appears at the medial tips of frontonasal prominence.

Principle

Different ions migrate at different speed dictated by their size and by the amount of charges they carry. As a result, different ions can be separated from each other by electrophoresis. The magnitude of the electric force F exerted on the charged particle when subjected to electrophoresis is product of the charge q of the particle and the electric field E generated between the two electrodes.

Plant parasitic nematodes favour the establishment of secondary pathogens viz., fungi, bacteria, virus etc. The nematodes alter the host in such a way that the host tissue becomes suitable for colonization by the secondary pathogens. Even though the nematodes themselves are capable of causing considerable damage to the crops, their association with other organisms aggravate the disease. The nematodes cause mechanical wound which favours the entry of microorganisms. In some cases, the association of nematode and pathogen breaks the disease resistance in resistant cultivators of crop plants.

Nematode - Fungus Interaction

Nematode - fungus interaction was first observed by Atkinson (1892) in cotton. Fusarium wilt was more severe in the presence of Meloidogyne spp. Since then the nematode - fungus interations had received considerable attention on important crops like banana, cotton, cowpea, brinjal, tobacco and tomato. Some examples of nematode - fungus interaction are given in the following table.

2.1 INTRODUCTION

Several propagation structures are helpful in propagation of horticultural crops. These structures are helpful for seed germination, rooting of cuttings, layering, grafting etc. and other hardening operations. The structures which facilitate propagation of plants are called propagation structures. Propagation structures are required for propagating plants by seed, cuttings and grafting.2.2

GREENHOUSE

Greenhouse is a structure covered with glass or polythene for protection against adverse climatic conditions as well as to provide optimum environment for growth of the plants. Greenhouse has been used long back by horticulturists as a means of forcing rapid growth of plants and extending the growing season particularly in colder areas. Plants are grown or cuttings are rooted in a greenhouse when the atmospheric condition does not permit for raising them in outdoor conditions. Modern greenhouses are equipped with regulatory mechanisms for controlling temperature, light intensity, air, humidity etc. Plants in green house are grown in the ground bed or bench type raised beds. The size of the greenhouses and dimensions of beds vary according to the need of propagators. Glass covered greenhouses are expensive but they have long life. However, for short-term benefits, plastic covered greenhouses can also be made. Plastic covered greenhouses are lighter than glass covered ones but there is increase in humidity is such houses, especially in winters, which results in undesirable water drops on the plants. It can however be overcome by making adequate ventilation. Polyvinyl chloride (PVC) has also been used in construction of greenhouses but it tends to darken pre-maturely in sunlight. In India, construction of temporarily low-cost poly houses is in fashion for raising nursery of fruit plant in off-season. Such low-cost greenhouses are constructed either on wood or metal framework and are covered with polyethylene sheet of 0.10 to 0.15mm thickness, which is resistant to ultra-violet rays. These houses are equipped with thermostat, cooler or an air conditioner or humidifier etc for rigid control on temperature and humidity. Greenhouses made from fibre glass sheet are more durable than the polythene houses, but are quite expensive. In greenhouses, two types of beds are used for raising seedlings. In greenhouses, the beds may either be prepared on ground itself or raised beds or bench type beds are used. Greenhouse structures vary from elementary home constructed to elaborate commercial installations.

1. Botanical name :Machilus edulis King ex Hook.

2. Family : Lauraceae

3. Common Name: Pumsi, wild avocado

4. Name in Indian languages : Pumsi, Phumkung (Lepcha), Lachyphal, Kawala (Nepali).

5. Description

Pumsi is an evergreen species grow up to a height of about 15-20 m, with spreading branches. It grows from Nepal to Sikkim, Bhutan, Arunachal Pradesh and the whole north-eastern region. Pumsi is considered as one of the forms of Avocado (Percea americana) in its native region.

6. Origin and distribution

It is native of India,Nepal, Bhutan, Sikkim, Arunachal Pradesh and the whole north-eastern region. The plants growwell incool humid regions in hills.The species is found in low density in forests at 1200 - 2400 metres height.The species is hermaphrodite, suitable for light sandy to loamy and clay soils. It can grow in semi-shade. It prefers moist soil.