eChapters

Management of Angora rabbit includes important aspects of breeding, feeding, heeding and disease control.

8.1. Reproduction

Angora rabbits of lighter breeds reach breeding age of 6-7 m, medium rabbits at 7-8m and heavier breeds at 8-10 m. Female Angora rabbits do not have a true oestrus cycle with a definite heat period. They may mate at any time of year. Ovulation in does is induced by mating and occurs about 10 -12 hours later to mounting and copulation with the bucks. No doubt there is no definite heat cycle in Angora rabbits, yet during certain periods breeding does are more receptive to bucks than other times of the year. Some of the signs of such receptive period are restlessness, rubbing of chin on the feeding or watering bowls and vulva may be slightly swollen and purplish red.

Health care and preventive hygiene in rabbits are very important for the productivity of the rabbits and economical viability of a farm.

It is always better to prevent the disease rather than to get an animal treated, which is usually quite expensive. As such it is desirable that the rabbits are checked daily. The health and ailment signs can be recognized from the following things:

6.1. The Production Cycle

As ovulation in does is induced by mating and the females are generally kept in different cages from the males, it is the breeder who determines the reproduction rate of the unit. These rates vary from one or two litters a year under the most extensive management to eight to ten litters in an intensive management system. Does are remated either immediately after kindling (intensive system) or about ten days later (semi-intensive). In the semi-intensive system, the does are first presented to the buck at four and a half months. They are then mated 10 to 12 days after the birth of each litter. Weaning takes place at 30 to 35, or even 37 to 38 days. Extensive systems are characterized by a long delay between kindling and mating, and perhaps even until weaning. For example, the young may be weaned at 56 days and the doe mated after weaning.

5.1. Anatomy of the Genitals

5.1.1. Male reproductive system

Testes in the male are oval-shaped present within the scrotum which remain in communication with the abdominal cavity, where they were at birth. The rabbit is actually able to withdraw its testes when frightened or fighting with other males (Fig. 5.1). The testicles descend at about two months. The short, back-slanting penis points forward when erect.

4.1. Digestive System of Rabbit

The digestive system of rabbit includes:
    A.    The digestive tract 
    B.    Its associated glands
The digestive system breaks down the complex molecules into the simpler one so that it is readily absorbed from gastro intestinal mucosa.
 
A. The digestive tract (gastro intestinal tract/ alimentary canal)

The digestive tract is essentially a tubular structure for the ingestion and digestion of food. In an adult (4 to 4.5 kg) or semi-adult (2.5 to 3 kg) rabbit the total length of the alimentary canal is 4.5 to 5 m. It commences from mouth and terminates in anus. G.I.T is divided into following parts:

The success of rabbit farming depends upon the location and design of the rabbit house. Rabbit is a burrowing animal and has sharp reflexes. It is easily disturbed by any new and sudden noise. The farm should be located in a peaceful environment away from the hustle and bustle of human population.  A good housing facility with adequate arrangement of ventilation is the most important factor in rabbit house.

Domestication of the rabbit is relatively recent and most of today’s breeds have been bred by man in the last 200 to 300 years.

The rabbit has been used as an experimental animal in genetics, reproduction and physiology since the beginning of the century, but it was not until 1950 that the first findings on quantitative genetics were published in Venge’s study of maternal influence on rabbit birth weight.

1.1. History and Origins of Rabbits

The existence of rabbit with human being remained from time immemorial. Romans were the first to domesticate wild rabbits in the 5th century by the monks of the Champagne region in France as a readily available food source. Rabbits were introduced to Britain during the 12th Century, and during the Middle Ages, the breeding and farming of rabbits for meat and fur became widespread throughout Europe. Sources suggest that some women among the Medieval gentry even kept rabbits as pets. The selective breeding of European rabbits meant that distinct breeds arose in different regions, and the origins of many old breeds can be traced back several centuries.

Tools of Public Awareness

The public awareness and awakening can be created among people through various means and ways. In this age of computers and communication, science and technology, information and advertisements there are several quick and effective means of spreading news. The electronic media, the press, education institution, religious, adult education, moral education, spiritual education, religious discourse etc. all are essentially complementary to each other. The governments and the policy makers can only work forwards environmental preservation on political basis. The media, if work passively on pro environmental issues, awareness can be effectively generated among people.

16.1 What is Sustainable Development?

Sustainable development (SD) is a pattern of resource use that aims to meet human needs while preserving the environment so that these needs can be met not only in the present, but also for generations to come (sometimes taught as ELF-Environment, Local people, Future). The term was first  coined by the Brundtland Commission and defined sustainable development as “development that meets the needs of the present without compromising the ability of future generations to meet their own needs” (UN, 1987; Smith and Rees, 1998)

The acts for Environment Protection are :

        The Air (Prevention and Control of Pollution) Act
        The Water (Prevention and Control of Pollution) Act
        The Wildlife Protection Act
        Forest Conservation Act
        Environment Impact Assessment (EIA)
        Citizens Actions and Action Groups
        Public Awareness and Awakening

14.1 What is Education

Education is a systematic attempt towards human learning. All learning is subjective and self related. It is neither literacy nor information. Knowledge can never be learned. Knowledge is the fruit of experience and experience is the sensation of the individual. Individual experience is the internal function and is the function of awareness and understanding. And, one of the processes of knowledge and raising awareness is to be able to identify and clarify our values. Now values in education are essential to help each one of us directly so that we may order our relationships to the environment that lies outside us. Once we are clear about values, we shall be better able to shift and control information of the natural world, make wise choices and decisions, and be creative in our mental processes.

India is home of 16.7 % of the world population living in just 2.4 % of the worlds total land area. The decennial increase in population (1991-2001) has been 18.1 equivalents to the total population of Canada, France and Germany. The population in this century has 1.6 billion by 1960.

12.1 What is Climate Change?
 
The earth receives short-wave radiation from the sun, one-third of which is absorbed by the atmosphere, ocean, ice-land and living organisms. The energy absorbed from solar radiation is balanced, in the long-term, by the outgoing radiation from the earth and atmosphere. While short-wave radiation from the sun can easily pass through the atmosphere, the long-wave radiation emitted by the warm surface of the earth is partially absorbed by trace gases in the atmosphere called greenhouse gases’ (GHGs).

11.1 Global Warming

Definition : Global warming is defined as an increase in the earth’s temperature due to the use of fossil fuels and other industrial processes leading to a build-up of green house gases (CO2, methane, ozone, nitrous oxide, and chlorofluorocarbons) in the atmosphere.

11.2 Who Coined the Term Global Warming?

The principle of global warming was first put fourth by S. Arrhenius in his paper on “the influence of carbonic acid in the air upon the temperature of the ground” in 1869. This concept gained ground in the late 1980s and early 1990s when it was found that the earth’s temperature had warmed over the past century, because of increased carbon dioxide emission.

In November 1992 a document entitled Warning to Humanity was released, signed by 1500 scientists from around the world, including 99 Nobel laureates, and the Director General of the United Nations Educational, Scientific and Cultural Organization (UNESCO). The document stated that “human beings and the natural world are on a collision course,” which “may so alter the living world that it will be unable to sustain life in the manner that we know.”

9.1 What are Natural Disasters?

The natural disasters are earthquakes, floods, droughts, famines, heat waves, cyclones, wild fire, volcanic, aerosols (small particles), and landslides.

9.2 Disaster Management

Both physical solutions and institutional strategies should be developed to manage natural disasters. For flood disaster control the water flow should be checked. Institutional strategies do not rely on physical barriers and include flood plain management, flood and disaster warning systems, education and preparedness programmes.

To a layman, wasteland is a piece of land, which is lying uninhabited or uncultivated. These lands are also commonly perceived to produce much less than their potential. Depleted of the green cover and productivity, such lands are often in a critical condition. Broadly, wastelands may be defined as land where the production of biomass is less than its optimum ‘productivity. Wasteland also refers to lands, which are economically unproductive, ecologically unstable and subject to environmental deterioration.

7.1 What is Pollution?
 
Pollution is a contamination of the environment by man-made substances or energy that has adverse effects on living or non-living matter. In simple terms, pollution can be seen as the wrong substance in the wrong place in the wrong quantities at the wrong time. This implies that harm is caused to the environment if the same substance is present at levels too low.

Types of pollution
Pollution can be categorized according to the medium in which it occurs such as

6.1 Biodiversity

Biodiversity means biological diversity. It measures the variety of the earth’s animal, plant and microbial species; of genetic difference within species and of the ecosystems that support the species in its widest. Biodiversity is virtually synonymous with “life on earth”, this word was coined in 1985. Biodiversity is the degree of variety in nature and not nature itself.

5.1 Concept of Ecosystem

Ecosystem is the basic functional unit in ecology, since it includes both biotic and abiotic environments influencing each other for maintenance of life.

A.G. Tansky in 1935 first proposed the term ecosystem. According to him, it is defined as the system resulting from the integration of all the living and non-living factors of the environment. In a simplest form, an ecosystem can be defined as a self-sustained community of plants and animals existing in its own environment. The term ecosystem is composed of two terms, “eco” means environment and “system” means an interacting and interdependent complex. It is otherwise known as bio system.

In simple words natural resources can be defined as any part of our environment such as land, air, minerals, forest, rangeland, wild life, fish and human beings.

The sum total of all physical, chemical, biological and social factors which compose the surroundings of man is referred to as environment and each component of these surroundings constitute a “resource”.  The natural resources are land, water, air, minerals, forests, rangeland, wild life or even human beings known as natural resource.

The five basic natural resources are energy, matter, space, time and diversity.

3.1 What is environment ?

The word environment is derived from a French word “environne” means to encircle or surround. Environment is defined as the circumstances or conditions that surround an organism or group of organisms. It is also defined as the complex of social or cultural conditions that affect an individual or community. Since humans inhabit the natural world and they build social, cultural and technological world, all constitute important parts of our environment.

Abiotic Environment

The physical or abiotic environment includes all those physical and non-living chemical aspects, which exert an influence on living organisms. The abiotic components are soil, water, air and energy from various sources. These provide both habitation and raw material for the synthesis of organic food.

1.1 Man’s place in Nature

All religions of the world share the view that man is the supreme being among God’s creations. He is the only one among many creatures to enjoy nature’s beauties and bounties. Nature is given to him so as to make responsible and judicious use of it. No religion justifies the most destructive attitude the modern man has developed towards his environment. All religions urge man to treat nature as a God-given gift, stating that God is omnipresent in the manifestations of the nature. It is irreligious for man to expect miracles and exploit the environment. Man must be confronted with the reality that struggle is essential for life rather than “struggle for existence.”

In nature, microbes are found as a mixed population. The types that encounter the most favourable environmental and other growth conditions emerge in abundance while others that find the environment less favourable are fewer in number. However, for any kind of study on them or for their use as a pure species, they have to be separated from other species. The nutritional media or culture media are different for different species. As the medium is specific for certain species, it checks or restricts the growth of other microbes for which it may not be suitable. However, some growth of and contamination by unwanted species may occur.

Biofertilizer is still an unclear term. It can be easily found that biofertilizers are identified as plant extract, composted urban wastes, and various microbial mixtures with unidentified constituents, and chemical fertilizer formulations supplemented with organic compounds. Likewise, the scientific literature has a very open interpretation of the term biofertilizer representing everything from manures to plant extracts.  Quality control of biofertilizers involves several major aspects such as isolation and identification of various strains, screening of the pure isolated strains, fermentation and quality control and finished product.

14.1 Introduction

The green revolution brought amazing consequences in food grain production but with insufficient concern for agricultural sustainability. The availability and affordability of fossil fuel based chemical fertilizers at farm level in India have been ensured only through imports and subsidies which are largely dependent on GDP of the country. Dependence on chemicals for future agricultural needs would result in further loss in soil health, possibilities of water contamination and calculated burden on the fiscal system. Indiscriminate synthetic fertilizer usage has polluted the soil, water basins, destroyed micro-organisms and eco-friendly insects, made the crop more susceptible to diseases and depleted soil fertility at the primary levels as of today (Pindi, 2012). India is an agricultural based country.

Earthworms have been on the earth for over 20 million years. In this time they have faithfully done their part to keep the cycle of life continuously moving. Their purpose is simple but very important. They are nature’s way of recycling organic nutrients from dead tissues back to living organisms. Many have recognized the value of these worms. Ancient civilizations, including Greece and Egypt valued the role earthworms played in soil. The Egyptian Pharaoh, Cleopatra said, “Earthworms are sacred.”

Most trees legumes thrive in tropical regions but N2 fixing non leguminous trees are prevalent in temperature conditions. Callatiam and coworkers from U.K. cultivated Frankia for the first time in 1978 in pure culture. A number of non-leguminous shrubs and trees as Alnus, Myrics, Hippophae, Casuarina and Elaeagnus are nodulated by N₂ fixing Actinomycetes, Frankia with Frankiaceae (Actinomycetes), the only acyinomycete reported to fix nitrogen to date. The so called actinorhizal plants include 7 orders, 8 families, 17 genera and 173 species of dicotyledons plants reported by Gaur, (2010), whereas 200 plants and 23 genera were reported by Diagne et al. (2013). Some of the important non-leguminous families and genera are given in Table 12.1.

11.1 Introduction

Our Country is a fast developing nation for the overall development and has proved that the country which was known for a begging bowl for food grains has turned up towards self-sufficiency and also has excess production to feed the present level of population. From about 50 MT of food grain production in 1950-51, harvest in 2010-11 reached over 241 MT, highest ever along with high production of fruits, vegetables, milk, egg and fish. This increased food production in the country has mainly been achieved through productivity gains, as the net sown area has remained static for the last four decades.

Phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement (Donahue et al., 1990), which is required in optimum amount for proper growth of plants and soil micro-organisms. Being a constituent of ATP, it is involved in various processes such as cell division, energy transduction through photosynthesis and biological oxidations and uptakeof nutrients. The average soil contains 0.05% phosphorus, but only one tenth (1/10) of this is available to plants due to its poor solubility and chemical fixation in soil (Barber, 1984). About 98% of Indian soils have inadequate supply of available phosphorus (Ghosh and Hassan, 1979). A survey of Indian soils showed that out of 363 districts, only 2.2% were high, 51.5% medium and 46.3% were low in phosphorus content (Ghosh, 1982).

BGA belong to a class of prokaryotic photosynthetic microorganisms also known, as cyanobacteria are capable of fixing atmospheric nitrogen aerobically. Most abundantly found in tropical zone, never form fibrous growth and are enveloped by a sheath. The trophic independence of algae for C and N makes them responsible for a natural fertility buildup of rice field soils. The nitrogen fixing ability of BGA was first recognized by Frank and Prantil in 1889. The occurrence of the BGA in the rice field was reported by FE Fritsch in 1907.

Azospirillum is a spiral-shaped nitrogen fixing bacterium. It also produces hormones and vitamins. Important species are Azospirillum brasilense and Azospirillum lipoferum. It is widely distributed in soils and grass roots.The genus Azospirillum belongs to family Spirillaceae with four commonly available species viz. A. brasilence, A. lipoferum, A. amazonense and A. halopraeferens or A. seropedicae (Fig. 8.1).

The first species of the genus Azotobacter, named Azotobacter chroococcum family Azotobacteriaceae, was isolated from the soil in Holland in 1901. Azotobacter represents the main group of heterotrophic free living nitrogen-fixing bacteria principally inhabiting neutral or alkaline soils. Azotobacter chroococcum and Azotobacter agilis were studied by Beijerinck (1901). In subsequent years several other types of Azotobacter group have been found in the soil and rhizosphere such as Azotobacter vinelandii, Lipman (1903); Azotobacter beijernckii, Lipman (1904); Azotobacter paspali, Döbereiner (1966), Azotobacter nigricans, Krasil’nikov (1949); Azotobacter armenicus, Thompson and Skerman (1981); Azotobacter salinestris, Page and Shivprasad (1991). A. paspali while A. agilis was categorized  under the genus Azomonas (Table 7.1).

Azolla is a free-floating water fern that floats in water and fixes atmospheric nitrogen in association with nitrogen fixing blue green alga Anabaena azollae. Azolla fronds consist of sporophyte with a floating rhizome and small overlapping bi-lobed leaves and roots. Rice growing areas in South East Asia and other third World countries have recently been evincing increased interest in the use of the symbiotic N2 fixing water fern Azolla either as an alternate nitrogen sources or as a supplement to commercial nitrogen fertilizers. Azolla is used as biofertilizer for wetland rice and it is known to contribute 40-60kg N/ha per rice crop. The agronomic potential of Azolla is quite significant particularly for rice crop and it is widely used as biofertilizer for increasing rice yields. Rice crop response studies with Azolla biofertilizer in the People’s Republic in China and in Vietnam have provided good evidence that Azolla incorporation into the soil as a green manure crop is one of the most effective ways of providing nitrogen source for rice.

The role of leguminous plants in improving the soil fertility is known for centuries. The nitrogen uptake in legumes by some unknown process was demonstrated through agronomic experiments by J.B Boussingault in 1838, which was opposed by Liebig and others. The presence of bacteria in the roots nodules of legumes was observed by P.G Lachmann (1858). M. Woronin (1866) further examined and observed them to be rod-shaped and motile.

Under agricultural conditions, the nitrogen removed is usually greater than the nitrogen input. To maintain fertility, nitrogen must be returned to the soil. This takes place mainly by biological nitrogen fixation, which is responsible for the major reduction of dinitrogen to ammonia that is used by the plants. Biological nitrogen fixation accounts for most of the fixation of atmospheric nitrogen in to Ammonium, thus representing the key entry point of molecular nitrogen into the biogeochemical cycle of nitrogen (Fig.4.1). Some bacteria can convert atmospheric nitrogen into ammonium. Most of this nitrogen fixing prokaryote is free living in the soil.

Biofertilizers can be defined as the preparations containing strains of micro-organisms which can augment the microbiological process viz. nitrogen fixation, phosphate solubilization or mineralization, excretion of plant growth promoting substances or cellulose or lignin biodegradation in soil, compost or other environments. Biofertilizers can be divided into nitrogen fixing bacteria, phosphate solubilizing and mobilizing microorganisms and organic matter decomposers as (Table 3.1 and Fig. 3.1):

On the basis of Nitrogen Fixation: On the basis of N₂ fixation, they are divided in to two sub categories as

2.1 Introduction

Microbes are the oldest form of the life on earth. These organisms date more than 3 billion years to a time when they were discovered with oceans. They are so tiny in size that million microbes can fit into the eye of a needle. Without microbes, we can’t live. Thus, understanding microbes is vital to understanding past and future. Microbes play many roles in the earth’s environment recycling dead plant and animal matter through the soil, removing CO2 from the atmosphere by photosynthesis in the oceans and nitrogen from the atmosphere to form nitrogenous fertilizers plants. Microbes play an important role in nutrient recycling, management, organic matter, decomposition and increasing the crop productivity and quality and protection against diseases (Panwar et al., 2009). Over the past century, many of the basic scientific principles of plant nutrition and soil fertility have been explained. In developed countries, inorganic chemical fertilizers have been widely accepted as a major source of improving and maintaining soil fertility.

Agriculture is undoubtedly the largest livelihood provider in rural India. As we enter a new post WTO Scenario and IT revolution, new inventions are happening day by day in every sphere. Agriculture is not culture now, it is agri business! Agriculture constitutes over 25% of India’s GDP. In recent years, much emphasis has been given by the Ministry of Agriculture on commercializing agricultural production in the country. Adequate production and distribution of food has become a high priority and global concern. In the fast changing world and increasing competition in a globalized economy, there is a need for exploiting the available resources to the maximum level and use of best technologies available world over, to cope up with domestic demand of food and also to target export market. This will be particularly relevant in South India, where farmers grow a number of crops, but have technical constraints in enhancing production and productivity because of inadequate exposure to high technology & inputs coupled with advanced production practices, logistics and marketing. In addition, shortage of labour is a major concern for progressive farmers of South India who are ready to offer more wages to labourers. Therefore, they are forced to become machinery-dependent in their farm operations. As a result, Southern India is emerging as one of the hottest destinations as well as a strong market with buying potential for farm machinery and equipment.

The market of organic products is growing globally every year. This organic market expansion makes it possible for farmers to reap the benefits of a trade with relatively high price premiums. Organic farming is carried on in almost all countries of the world. The share of organic farms in overall farmland and in terms of numbers of holdings is growing continuously. The market for organic products is also growing very rapidly, not only in Europe, North America and Japan, where the largest markets are to be found, but also in many emerging economies in transition. In India, an organic movement is now emerging on different levels i.e. producer groups, trainers and advisors, certification bodies and processors and traders.

The organic quality is based on standards, inspection, certification and accreditation. All organic food is produced and handled according to strict rules called ‘Organic Standards’. Organic standards do not define a quality status which can be measured in the final products (e.g. quantity of pesticides residues, heavy metals, etc). They define the way of production (e.g. that no chemical pesticides and fertiliser are used).

The problem of insect-pest, disease and weed is acute in case of all the crops and especially so in case of commercial crops. Pesticides, fungicides or chemicals are meant to control harmful pests such as insects, nematodes, diseases, weeds etc. However, excessive use of pesticides, fungicides and herbicides not only leave residues in soil, water and air but also have adverse effects on the non-target organisms such as pollinators, parasitoids, predators and wild animals. This has adversely affected the ecological balance resulting in pest resurgence, development of resistance in the pest species, diseases and environmental pollution.

Plant nutrients are drawn up from soil for growth and development. There are more than 90 elements present in the soil. According to the criteria of Essentiality of Nutrients for plant growth, proposed by Arnon and Stout in 1952, only 16 are considered to be essential for plant growth. Out of these, C (Carbon), H (Hydrogen), O (Oxgen), N (Nitrogen), P (Phosphorus), K (Potassium), Ca (Calcium), Mg (Magnesium) and S (Sulfur) are called Macronutrients (required in large quantities) and the remaining 7 elements - Fe (Iron), Mn (Manganese), Cu (Copper), Zn (Zinc), B (Boran), Mo and Cl are called Micronutrients or trace elements (required only in very small quantities). Among the macro elements - N, P and K are called Fertiliser elements or primary nutrients as they are most often applied in the form of synthetic or mineral fertilisers, and Ca, Mg and S are called secondary elements which are applied in smaller quantities than primary nutrients and required subsequent to primary nutrients.

Organic food production is as much a state of mind as it is a production system. The organic farmer must understand how everything is interrelated and how one set of circumstances will influence other factors in how the plant grows. A strong, healthy plant is much more important in an organic system then a conventional system. A two to three year leaning curve must be accepted by anyone intending to switch from conventional to organic farming. What works one year may fail the next.

The need for organic farming in India arises from the unsustainability of agriculture production and the damage caused to ecology through the conventional farming practices. The present system of agriculture which we call ‘conventional+’ and practiced the world over evolved in the western nations as a product of their socio-economic environment which promoted an over riding quest for accumulation of wealth. This method of farming adopted by other countries is inherently self destructive and unsustainable.

Farming is the intervention of man in the growth cycle of plants, animals or birds to get utility products of choice at maximum levels and of highest quality. The growing and caring of plants is called Crop Husbandry, and that of animals is called Animal Husbandry. Farming is our most basic activity. It is considered as the backbone of Indian economy. Farming not only nourishes the whole population, but also provides a means of livelihood to more than 60 per cent of our population. Our history, culture and community values are embedded in farming. Today when we think of farming, what comes to our mind are long stretches of farm land, farmer tilling the field, pumping water into fields, applying fertilizers or pesticides, plants with so many fruits, tractors with loads of farm produce, cross bred cows, a farm chicken, aquaculture, fish and so on. A more advanced image on this subject will be of the small plantlets in conical flasks or test tubes, what we call Tissue culture, High Yielding Varieties (HYV), Genetically Modified (GM) crops, Green House farming and other techniques that increase the crop yields. So all these activities are considered as the products of science that our scientists were able to develop over a period of time and helped the growing population to meet their food requirements.

PGPR have shown potentiality against pests and diseases oc curring on crop plants. Wilt and collar rot diseases are caused by soilborne pathogens viz., Fusarium oxysporum fsp. lycopersici (FOL) and Sclerotium rolfsii respectively in vegetables. An attempt was made to manage the diseases with PGPR microbes and 142 PGPR strains were isolated from different vegetable crops. Among them two isolates viz., Bacillus amyloliquefaciens (BA1) and B. subtilis (BS2) were found highly effective in controlling the diseases.  Apart from the disease control, the isolates BA1 and BS2 also registered enhanced crop growth and yield in cowpea under field conditions. Species level identification of BA1 and BS2 was done through PCR amplification and sequencing of 16s rRNA. Both BA1 and BS2 clustered with Bacillus amyloliquefaciens and B. subtilis respectively.

Mastitis is a global problem as it adversely affects animal health, quality of milk and economics of milk production. Bovine Mastitis is reported to be the most costly disease of dairy cattle, causing huge economic losses in terms of reduced milk yield, milk discard after treatment, cost of veterinary services and premature culling. While reviewing status of mastitis in India, subclinical mastitis is more important (10–50%) than the clinical mastitis (1-10%). Estimated annual economic losses incurred due to mastitis estimates to Rs.7165.57 crores to dairy farmers (Bansal and Gupta, 2009).