eChapters

The diets of over two-thirds of the world’s population lack one or more essential mineral elements. This can be remedied through dietary diversification, mineral supplementation, food fortification, or increasing the concentrations and/or bioavailability of mineral elements in produce (biofortification) involving aspects of soil science, plant physiology and genetics underpinning crop biofortification strategies, as well as agronomic and genetic approaches currently taken to biofortify food crops with the mineral elements most commonly lacking in human diets: iron (Fe), zinc (Zn), copper (Cu), calcium (Ca), magnesium (Mg), iodine (I) and selenium (Se).

It is now a fait accompli that for enhancement of the economic condition of the farmers, just growing of the variety of crops is not sufficient. Along with the crop husbandry, it is the need of the time that farmers must integrate other enterprises with traditional crop production practices. Integration of various farm enterprises in a farm ensures growth and stability in overall productivity and profitability. It also ensures recycling of residues, optimization of resources, reduction of risk and generation of employment. Various enterprises that could be included in farming system are agronomical crops, vegetables, fruits, flower cultivation, dairy, poultry, fishery, goat keeping, piggery, sericulture, mushroom cultivation, agro forestry, bee keeping, silviculture, agro-based industries and food processing. A judicious mix of enterprises complementary to crops and suited to the given farm situations and farmer’s preference would bring overall prosperity.
 
The Integrated Farming System (IFS)  is a complex inter-related matrix of soil, plant, animals, implements, power, labour, capital and other inputs controlled in part by farm families and influenced by varying degrees of political, economic, institutional and social forces that operate at many levels. Under farming system, the farm is viewed in a holistic manner. Farmers are subjected to many socio-economic, bio-physical, institutional, administrative and technological constraints. Integrated farming system conceptually is a set of elements or components that are inter-related with each other and which are interact among themselves.

Introduction

Fresh fruits and vegetables are known for their nutritional and relishing nature. But, their fast perishability and season wise availability make them unavailable for a longer time. To enjoy and get benefited from their unique taste and nutritional value they are further preserved or converted into some value-added products such as jam, jelly, candies, juices, minimally processed fresh-cut fruits and vegetables, pickles etc. There are techniques which are developed to preserve them while in fresh and also when they are made into value-added products. Some require only one technique and other requires more than one in combination so that the product can be preserved for a longer period and available to maximum population even after their period of production/availability is completed.

Introduction

The Intergovernmental Panel on Climate Change Fifth Assessment Report (IPCC, 2013) emphasized that warming of the climatic system is unequivocal and that anthropogenic warming will continue for centuries to come due to timescales associated with climate processes and feedbacks. While stresses caused by climate change are likely to negatively impact the agricultural and livestock sector and escalate existing small-scale farming challenges; agricultural and livestock productivity and sustainability have to improve to meet the needs of the growing human population (DEA, 2013; Zamykal and Everingham, 2009). Climate change is seen as a major threat to the survival of many species, ecosystems and the sustainability of poultry production systems in many parts of the world. Nevertheless, global demand for poultry products is expected to increase, as a result of the growing human population, and its growing af?uence. The extreme climatic conditions impose various stresses on poultry birds which adversely affect their growth, production and reproduction.

While climate change is a global phenomenon, its negative impacts are more severely felt by poor people in developing countries who rely mostly on the natural resource base for their livelihoods. Moreover, rural poor communities rely greatly for their survival on agriculture and poultry which belong to the most climate-sensitive economic sectors. Climate change is expected to intensify existing problems and create new combinations of risks and shift hazard zones. The incidences of draughts, snow-storms and blizzard like events have risen. The situation is made worst due to factors such as widespread poverty, inequitable land distribution, limited access to capital and technology inadequate infrastructure, long term weather forecasts and inadequate research and extension. Intensive poultry production has much less impact on global warming than organic or free-range production (Van der Sluis, 2007). Seo and Mendelsohn (2006) reported that small farms of poultry are better able to adapt to warming. However, organic egg production needs more energy than non-organic and increases most environmental burdens. The most disadvantageous, from environmental point of view, is litter-free breeding of birds which causes great amounts of liquid manure.

Bone marrow contains variety of tissues including hematopoietic lineage cells and mesenchymal stem cells (MSCs). The hematopoietic cells are the major source of the blood cells in the adult body which are regulated within the microenvironment of the stromal cells of the bone marrow. MSCs are multipotent cells and can be differentiate into various cell lineage depend upon their environment and culture condition in which they are kept. Rat bone marrow derived stem cells (rBM-MSCs) have long term self renewing and capabilities of pluripotency including osteoblast, adipocytes, chondrocytes, tenocytes, muscle cells and neurogenic cells, which make them ideal source of stem cells for regeneration of injured tissue.

Bone marrow derived mesenchymal stem cells have been isolated from different species. Each species is having different culturing and growing capacity. For example human bone marrow derived stem cells are easy to harvest and maintain in culture condition whereas rat MSCs it is more difficult compared to other species. Technical difficulties in isolation limited the number of animal experiment and its required animal transplantation model for pre clinical studies. Density gradient centrifugation, plastic adherence and immunomagnetic selection like various methods are available for isolation of MSCs from bone marrow. No appropriate methods are available for selection of suitable cells. Each method has their own pros and cons. Therefore, in this chapter, combined density gradient centrifugation and plastic adherence as easy and reliable method for isolation, culture and characterization of rat bone marrow derived mesenchymal stem cell is described.

Introduction

Cultural management is defined as manipulation /or adjustment of crop production techniques for management of plant diseases. Cultural practices have been as old as possibly agriculture itself. In early stage of agriculture development, the growers through their experiences and observations had known that repeated cultivation of a particular crop species or variety on a piece of land often resulted in crop sickness. As a matter of fact, in the present day agriculture, cultural practices are being considered as essential backup methods for management of plant diseases. Adequate adjustment in crop production techniques can modify the environment in such a manner that it becomes unfavorable for the pathogen and pathogenesis. Based on this the disease control affected by cultural practices are preventive, these practices aim at reducing the activity and density of inoculum. It offer the opportunity to alter the environment, the condition of the host, behavior of the causal agent to achieve economic management of disease.

Molecular diagnostics has witnessed groundbreaking technological advances in recent times, starting from automated sample preparation to real time amplification technology. Possibility to develop and run assays for ever increasing number of clinical queries has become effortless due to the technological advances. However, the state of the art in quality assurance and quality control practices for molecular diagnostics has seldom been up to the expectations if one considers accuracy for once in a lifetime genetic tests or new genetic test targets appearing almost every single day. In the face of such issues, clinical laboratories are struggling to develop appropriate quality assurance programs for the molecular diagnostic tests they conduct. In addition, legislation and government policies threaten to increase oversight of genetic tests to a level not seen before in laboratory medicine, for example, FDA’s guidance on in vitro diagnostic multivariate index assays (IVDMIA).

To relate patient results to previous test results or to absolute values in clinical practice guidelines, those results need to be comparable across time and methods. This may either be achieved by designing the identical value across methods and test versions or by using reliable and stable conversions. Therefore, the establishment of international standards and reference materials is of paramount importance. Monitoring molecular test system outputs and statistical analysis might provide a good way to obtain such standards; however, such traditional QC (Quality Control) strategies can be slow to take root in molecular diagnostics.This chapter highlights general and specific issues relevant for quality assurance and quality control in the routine molecular diagnostics laboratory and guidelines for designing quality manuals for a molecular diagnostic laboratory.

Introduction

We use our five senses to perceive the world around us. Some senses, like touch and taste, need contact with the objects. But we get most of our information about the environment through sight and hearing, which do not need close contact with the external objects. In scientific terms, we are doing remote- sensing all the time. Remote- sensing is the process of recording/observing/ perceiving (sensing) objects or events from a distance. Remote- sensing is a way of monitoring and detecting the features, usually physical, of a specific area by measuring its reflected and emitted radiation (usually from satellite or aircraft).

Introduction

Packaging is one of the most important processes to maintain the quality of food products for storage, transportation and end-use. The basic functions of packaging are considered as protection, containment, information and convenience. Food packaging was initially considered to increase the marketable life of the food in a container by protecting it from the influence of the environmental factors. Then it was expected to be informative, easy-to handle and disposal-friendly with increased functionality. Current innovations of food packaging technology are geared to ensure one/ all of these properties along with better protection, more efficient quality preservation and enhanced safety of the packaged food. Innovative packaging technologiesalso prevent the rate of quality deterioration and facilitate easy handling of the produce during distribution and marketing. During distribution, the quality of the food product can deteriorate biologically and chemically as well as physically. Innovative packaging technologies are the most important and challenging technologies to overcome these limitations.These technologies require specific knowledge and appropriate training and understanding for rationally selecting the most suitable packaging for each product and intended use. Innovative packaging of foods includes active packaging, smart packaging, on-the-go packaging, intelligent packaging etc. which have been briefly discussed in this manuscript.

Composition is a fundamental element of cinematography, essential for creating engaging video content. When designing shots and angles, videographers must consider not only technical aspects like lighting and sound but also creative choices that set the mood. Video composition helps filmmakers craft a narrative, influencing how viewers engage with the story. Effective composition goes beyond just framing; it involves color grading and the strategic placement of elements within the frame, making it vital for captivating the audience.

Video Composition Tutorial

A tutorial from B&H Photo’s YouTube channel outlines five crucial rules of video composition that should not be broken. These rules serve as a foundation, providing structure and consistency to the creative process

ABSTRACT

In last twenty years, molecular biology has revolutionized conventional plant breeding techniques in many areas. The use of molecular markers in conventional breeding techniques has also improved the accuracy of selection and allowed breeders to produce varieties with combined traits that were difficult before the advent of DNA technology. Among vegetables, onion (Allium cepa L.) is the most important cash crop of India. Onion productivity in India is much lower (11.38 tones / ha.) compared to other countries (44.1 tones / ha. in Japan). Purple blotch disease (PBD) caused by Alternaria porri (Ellis) Ciferri decreases the yields by up to 50 percent. Genetically resistant onion varieties are the most economical, simple and eco-friendly mode of disease management. The advent of mo- lecular markers provided the opportunity to understand the genetic resistance more pre- cisely. In this paper we present our successful efforts in developing PCR based markers linked to purple blotch disease resistance, so that such markers can be easily integrated into PBD resistance breeding program in onion. Onion germplasm was screened for PBD resistance. Line PBR 287 was identified as a good source of resistance and the genetics of inheritance of resistance in this line was worked out. The protocols for isolating good quality DNA and PCR amplification conditions were standardized. 37 polymorphic mark- ers were identified between resistant and susceptible parents. Crosses were made between resistant and susceptible parents. Parents, F₂ and individual F₂ progenies were subjected for analysis of genetics of resistance and RAPD marker analysis. F₂ individuals segregated in 3:1 ratio for resistance. DNA from resistant and susceptible F₂ individuals was pooled separately for Bulked Segregant Analysis (BSA). BSA identified one RAPD marker of 900bp being linked to resistance. Further Chi-square test for detecting the deviation of segregation of this 900bp marker confirmed segregation with classical Mendalian ratio 3:1 for a monogenic trait. Identified marker is of great practical utility to the plant breeders to screen large number of populations for purple blotch disease resistance at the early stages of the crop even in absence of the pathogen.

1. TECHNIQUES OF HYBRID SEED PRODUCTION

1.1. Field crops

Table 1: Techniques of hybrid seed production in field crops

Introduction

Ducks are domesticated before 3000-4500 years. Ancestor of domestic ducks is wild mallard duck (Anas boschos) which are broody. Scientific name of domestic duck is Anas platyrhyncos. Duck farming is the part of poultry farming system. Duck can be raised for meat and eggs. In most part of the world, duck ranks next to the chicken in terms of egg and meat production. Commercial duck farming is increasing as this business has good potential to earn money through egg production. Duck can be feed on agro-industrial waste, swamp and marshy areas. These hardy birds can survive in such as areas where no other agricultural crops could be grown and other livestock raised. Duck farming business is a very profitable business as there are many duck breeds available all over the world. For commercial duck egg production and breeding, must go through water sources for raising ducks. It will reduce cost of feed. Duck can survive without water; but they need water pond kind things for fertile egg laying and mating. If pond is available then it is well and good for commercial duck farming. Chromosome number of duck is 80. Duck sound is whimpering or betch, honk. Incubation period of duck egg is 28 days. Egg weight duck is 72 gm.

Tissue culture is a technique used to culture cells, tissues, organs or whole plant under controlled environmental conditions that in vitro condition is known as plant tissue culture. Plant tissue culture exploits the totipotency nature of plant cells, ability to develop in to complete plantlet.

ABSTRACT

As we all know that emergence of internet has completely changed the way of transferring knowledge. Online videos and lectures have been adopted by the online educational community to meddle into the MOOCs massively to disperse the knowledge. No field has remained untouched from MOOCs. This is basically a study on the available MOOCs in India in Library and Information science.

Keywords: MOOCs, Massive Open Online Course, SWAYAM, Library and Information Science.

1. Introduction

Dave Cormier devised the term Massive Open Online Course (MOOC) in 2008 to describe an open online course “Connectivism and Connective Knowledge” designed by Siemens and Downes. University of Manitoba offered MOOC for the first time in 2008 formally as a credit course to regular students and informally with enrolment open to anybody in the world free of cost. Since then, there is proliferation of MOOCs worldwide.

Gametogenesis

Male Gametogenesis: Male gametogenesis is the formation of male gametes and it occurs in the anthers. The end products of meiosis in higher plants are four microspores. Two mitotic divisions occur in microspores. The first division produces a tube nucleus and a generative nucleus. The second mitotic division occurs only in the generative nucleus resulting into production of two sperm cells (Fig. 3.1). The microspore is haploid (n) and develops into pollen grain in the anther, and the pollen grain further develops into a pollen tube on the stigma and in the style. The pollen tube, when it reaches the embryo sac is the fully developed male gametophyte and is trinucleate, two sperm cells and one tube nucleus.

Introduction

Spectral signature: A spectral signature is the unique pattern of reflection and emission of a target at different wavelengths. This difference helps to classify remote sensing images based on the spectral signatures of different objects.

Electromagnetic (EM) spectrum: The EM spectrum is the range of all types of EM radiation. Some examples are visible light from a lamp, radio waves from a radio station, microwaves, infrared light, ultraviolet light, X-rays, and gamma rays.

Spectral response patterns: A spectral response pattern is the amount of energy that an object reflects or emits at different wavelengths. It shows how the object interacts with the EM radiation.

Introduction

The term ‘Fish Marketing’ is composed of two broad meaning term ‘Fish’ and ‘Marketing’. Before discussing the methodological issues of fish marketing data analysis, we must understand the broader meaning of it as both words are being used frequently with a précised meaning. Google dictionary explains fish as ‘A limbless cold-blooded vertebrate animal with gills and fins living wholly in water’. But ‘fish’ as a component of fish marketing or social science study, means a product and a variety of product, which has different time, space and form utilities for consumers.

The word ‘marketing’ is not merely buying and selling of fish, rather it connotes a series of activities involved in moving goods or services from point of production to the point of consumption. So ‘fish marketing’ is defined as physical and institutional set up to perform all activities involved in the flow of fish, fish products and services from the point of initial fish production until they are in the hands of ultimate customers.

It includes assembling, handling, storage, transport, processing, wholesaling, retailing and export of fish and fishery products as well as accompanying supporting services such as market information, establishment of grades and standards, community trade, financing and price risk management and the institutions involved in performing the above functions.

It is desired to have an efficient and well planned distribution networkso that fish and fishery products move from the place of landing or production to the place of consumption at cheapest cost with quality –intact, ensuring the maximum price realization to the farmers or fish producers. It is really challenging to the public agencies involved to meet the requirements of all three major players, viz., fishermen or fish farmers, consumers and traders.

It includes all activities involved in creation of time, place, form and possession utility. Philip Kotler explained marketing as the science and art of exploring, creating, and delivering value to satisfy the needs of a target market at a profit. ‘Fish marketing’ is different from marketing of other goods and services and even differs a bit from marketing of variousagricultural goods and services.

ABSTRACT

The investigation aimed at to examine the diversity among nineteen wild and cultivated germplasms of the genus Vigna namely, V. trichuriensis, V. hainiana, V. radiata, V. aconitifolia, V. minima, V. sinensis, V. radiata var. sublobata, V. radiata var.setulosa, V. umbellata, and allopolyploid of BR3x T9 at DNA level follwing Random Amplified Polymorphic DNA (RAPD) technique. Ten random decamer primers were used in amplification reactions, five of that were reproducible and clear. The five primers constituted 55 bands of, which 9 are polymorphic. The similarity index based on 0 and 1 matrix of banding was calculated and dendrogram following UPGMA was constructed. The germplasms formed altogether 4 clusters on the basis of 0.375 linkage distance. The first and second cluster consisted of single genotype of V. hainiana. and V. minima respectively. The third cluster comprised wild and cultivated types of V. sinensis. The 4th cluster on the otherhand, consisted of genotypes belonging to V. radiata, V. radiata var. sublobata, V. mungo, V. radiata var. setulosa, V. trichuriensis and allopolyploid of BR3 and T9. The wild forms of different species were found to exist in the same cluster as their cultivated form. The three mungbean genotypes namely B105 (V. radiata), B1 (V. radiata) and BR3 (V. radiata )are crossable within themselves and they belonged to the cluster -IV.The intercluster cross between different clusters would like to generate new genotypes with wide variability. Based on limited range of accessions tasted in the present investigation, the approach holds promise for the classification of Vigna germplasm, identification of Vigna and marker assisted breeding in Vigna.