eChapters

Introduction

Measurement of pH of a solution and preparation of different types/strength of buffers are common practices in a laboratory, particularly in the Biochemistry and Molecular Biology laboratories. Therefore, in this chapter you will be acquainted with the procedures of measuring pH of a solution as well as preparation of some of the commonly used buffers in a laboratory.

Potential or Power of Hydrogen Ion (pH)

Measurement of pH of a solution is measuring the molar concentration of hydrogen ion [H+] in the solution, which is an indication of the acidity or basicity of the solution. The symbol pH stands for “power of hydrogen” and numerical value for pH is just the negative logarithm of the molar concentration of hydrogen ions [H+]. The ionic product of water (Kw ) is the basis for the pH scale. The total hydrogen ion concentration from all the sources and it can be experimentally measured.

The pH scale designates the concentration of H+ (and thus of OH−) in an aqueous solution, which ranges between 1.0 M H+ and 1.0 M OH−. The term pH is defined by the expression

Thus, the ionic product of water makes it possible to calculate the concentration of H+, with given concentration of OH−, and vice versa. The value of 7 for pH of a neutral solution is not an arbitrarily chosen figure; it is derived from the absolute value of the ionic product of water. A solution having pH greater than 7 is basic, and the concentration of OH− is greater than that of H+. A solution having a pH less than 7 is acidic.

Introduction

For upliftment of the Indian economy agriculture plays an important role as it has contributed immensely. Nearly, three quarters of India’s family depend on rural incomes. Majority of India’s poor live in rural areas. Most people depend upon the crops, vegetables, fruits grown by these rural population. India’s food security is depended upon the inputs made by the farmers which has been a reason for increasing food insecurity in India. To meet with this accelerated pace a productive, competitive, diversified and sustainable agriculture sector needs to be introduced (The World Bank, 2012).

India being a global agricultural powerhouse, is the world’s largest producer of millet, pulses and spices and also has the largest cattle herd in the world, India also produces largest amount of wheat, rice and cotton. Agriculture is a part of India’s culture and tradition. In India, the culture, tradition and festivals are in a way related to different crops grown by the farmers. Along with this agriculture is deeply rooted with the community lifestyle in India. The community freely share their knowledge related to growing, sowing and harvesting of crops, they also distribute or share the produced crops among each other freely (OECD, 2018).

Onion growth, bulb initiation, yield, quality and storage life are strongly influenced by temperature, photoperiod, soil, moisture and crop establishment. Different cultivars require specific day lengths and temperature ranges, making regional variety selection and planting schedules essential. This chapter explains the environmental requirements of onion and the consequences of unsuitable conditions, including premature bolting, double bulbs, poor development and disease susceptibility. It examines soil preparation, planting geometry, direct seeding, transplanting and precision establishment. Modern approaches—including sensor-based environmental monitoring, Internet of Things systems, predictive modelling, precision seeding and machine-learning-supported input management—are presented as tools for improving uniformity, resource efficiency and climate adaptability.

Land and water resources are central to agriculture and rural development, and are intrinsically linked to global challenges of food insecurity, land degradation , poverty, climate change adaptation and mitigation that affect the livelihoods of millions of rural people across the world. Current projections indicate that world population will increase from present figure of 6.9 billion to 9.1 billion in 2050. As a result, the world food demand will increase. Since, both land and water resources, the basis of our food production, are finite and already under heavy stress, so future agricultural production will need to be more productive and more sustainable at the same time. The largest contribution to increases in agricultural output will most likely come from intensification of production on existing agricultural land. This will require widespread adoption of sustainable land management practices, and more efficient use of irrigation water through enhanced flexibility, reliability and timing of irrigation water delivery. The prevailing patterns of agricultural production needs to be critically reviewed.

Q.1 If the sampling units of the population are of heterogeneous nature then stratified random sampling scheme is suitable for sample survey to be conducted.

Q.2 In stratatified random sampling scheme, the strata are homogeneous within themselves but heterogeneous between themselves.

6.1 Introduction

Plum is one of the important and widely cultivated fruit crops of temperate regions of the world. It occupies a unique position amongst the stone fruits in world fruit production and ranks next to peaches in economic importance. Plums are the most taxonomically diverse stone fruits which belongs to genus Prunus of sub-family Prunoideae and family Rosaceae (Potter et al., 2007). There are about 42 species of plum but the cultivated plums are derived from two species namely Prunus domestica (European plum) and Prunus salicina (Japanese plum). The Japanese plums are widely grown in the country whereas the European plums are grown only on a limited scale. The European plums are most often produced for the dried fruit market particularly in France and California, where it is called prune and dried plum, respectively. In the recent past some prunes have been introduced for fresh market in Himachal Pradesh. These prunes are sold fresh like plums and have become quite popular.

Introduction
Gastrulation is a vital stage in the development of multicellular animals because it establishes the basic body plan, including the primary body axes (dorsal/ventral axis and the cranial/caudal axis) and prepares the embryo for organ formation. The process of cleavage leads to formation of blastula which is followed by gastrulation. The blastula consists of inner cell mass (Cell mass made up of darker blastomere and present at animal pole which is going to form embryo proper) and trophoblast (Cells made up of clear cells and arranged periphery near to Zona pellucid). The morphogenic changes occur during gastrulation is the formation of three germ layers. Gastrulation is the process in which single- layered blastula is converted into a trilaminar structure consisting of an outer ectodermal, a middle mesodermal and an inner endodermal layer and occupy their characteristic position in embryo. They will be give rise to different organs and extra embryonic membranes. The gastrulation is an important step of cell differentiation.
Gastrulation in Mammals
The process of gastrulation is variable among different domestic animals. It takes place in the uterus when the blastocyst is floating in the uterine fluid. After the degeneration of zona – pellucida, the cells located in the ventral margin of the inner cell mass separate out and extended ventrally along the trophoblast layer. Due to further proliferation, splitting (delaminate) and migration of cells from the inner cell mass, form a complete cellular lining on the inner site of trophoblast. This newly formed layer is called endoderm corresponds to the hypoblast of chick blastoderm. The remaining cells of the inner cell mass correspond to the epiblast of chick blastoderm. With formation of endoderm the blastocoel is divided into 2 cavities. The portion enclosed within endoderm is called primitive gut and area between endoderm and trophoblast layer is extra embryonic coelom. During this time trophoblast cells covering the inner cell mass degenerate and compact inner cell mass exposed and it is called as embryonic disc The embryonic disc occupies a position at the animal pole. Note: The development of the germ layers in mammals closely resembles that of birds with the formation of the primitive streak and primitive node.7

Introduction

Water is the driver of life/ liquid of lift or elixir of life for flora and fauna. It plays an important role in plant as it constitutes about 35-95% part of plant. Water is a mode of transportation of nutrients from roots to shoots and vice-versa, a thermal regulator of temperature inside the plants, source of hydrogen for the reaction of CO₂ for photosynthesis and is essential for maintaining turgidity and servility of plants. Water is required for agriculture as well as for other sectors (domestic, industries, etc) and the demand of water is increasing alarmingly.  Presently agriculture consumes over 80 per cent of total water consumption in India. The country is endowed with many perennial and seasonal rivers. The river, low ever 71 per cent of water resources is concentrated in 36 % of the geographical at area. Water resource of India, population in per capita water ability and estimates of water needs are given below.

Abstract 

The district Murshidabad lies within the Younger Deltaic Plain and the Bhagirathi Recent Surface has been considered as the severe flood affected district of West Bengal state, India. Murshidabad is highlighted one in terms of occurrence of flood due to its typical geographic location and diverse geomorphological characteristics. The district witnessed frequent floods because of high intensity of rain fall in the basin area of Bhairavi-Jalangi-sealmari rivers. Inflow of flood water from Ganga-Padma in high spate condition further aggravates the congestion of drainage. Lack of proper drainage is also responsible for flood in the upstream side in the western part as well as beyond the Berhampur town. Flood management may be undertaken through physical and socio-economic measures, which includes the scientific control of reservoirs; construction of new barrages, improvement of river channels, removal of human encroachment along river side, timely flood warning and forecasting, preparedness of essential services along with proper flood plain planning are indispensable. Addressing of socio-economic problems may help to combat the flood situation in an effective way.

Marketing management refers to distribution of the firm’s product or service to the customers in order to satisfy their needs and to accomplish the firm’s objectives.

Marketing includes developing the product or service, pricing, distribution, advertisement, merchandising, doing personal selling, promoting and directing sales and service to customers.

Marketing is an essential function because unless the firm has a market, or can develop a market for its product or service, other functions of staffing, producing and financing are futile. Marketing functions are schematically given below :

Introduction

Since beginning of this century genetic diversity of agricultural crop has been lost due to several reasons (Marla, 2012). Genetic erosion is caused by human intervention, mono culture, green revolution especially due to commercialization of plant breeding. So there is urgent need to collect, conserve plant genetic & genomic resource for further use.

A seed bank stores seeds to preserve genetic diversity. There are many reasons to store seeds. One reason is to have on-hand the genes that plant breeders need to increase yield, disease resistance, drought tolerance, nutritional quality, etc. of plants used in agriculture (i.e., crops or domesticated species). Another reason is to forestall loss of genetic diversity in rare or imperiled plant species in an effort to conserve biodiversity ex situ and in situ. Many plants that were used centuries ago by humans are used less frequently now and seed banks offer a way to preserve that historical and cultural value. Collections of seeds stored at constant low temperature and moisture guard against loss of genetic resources that are otherwise maintained in situ or in field collections. These alternative ‘living’ collections can be damaged by natural disasters, outbreaks of disease or war. Seed banks are considered seed libraries and contain valuable information about evolved strategies to combat plant stress or produce novel products. The work of seed banks spans decades and even centuries. Most seed banks are publicly funded and seeds are usually available for research that benefits the public.

Whenever a thin hanging wire is loaded, it elongates and the load moves downwards. Increase in length of wire is governed by the magnitude of the load and nature of wire material. Experimentally it is found that the cohesive force between the molecules of hanging wire offers resistance against the deformation.

Introduction

Different types of culture media are used for culturing microbes and plant tissues. Preparing as well as sterilizing them properly following appropriate method with safety for the desired purpose are important parts of research work. In this chapter, we will discuss briefly about different types of media, their composition, preparation and sterilization in laboratory for microbial or plant tissue culture.

Culture Medium

Ingredients mixed to prepare solid, liquid or semi-solid substance to support the growth of microorganism or plant tissue in vitro (outside the body) is called culture medium. Frequently, it becomes important to grow microorganism or plant/animal tissue in vitro for any or all of the following purposes:

• To study characteristic features or properties of microorganisms.

• To identify the causal organism(s) of infection in a clinical sample, so that appropriate remedy can be determined.

• To culture/multiply microorganism or plant/animal tissues in vitro for research or commercial purposes.

• To prepare/produce biological molecules, vaccines, antigens, proteins, metabolites, etc. in vitro for research or industrial purposes.

Introduction

Among the various countries worldwide, such as China, Kenya, and Sri Lanka, India stands as a significant player. In 2016, China and India collectively accounted for over 60% of the world’s total tea production. Furthermore, India holds the distinction of being the foremost producer of black tea, while China and Japan lead in green tea production. In India, Assam and Darjeeling are recognized as the primary states for tea production, while the Nilgiris and North-East regions also contribute significantly, playing a crucial role in boosting the country’s economic growth.

Assam annually produces over 400 million kilograms of tea, making it the largest tea-growing region globally.Assam has the world’s biggest tea plantations, which are important for India’s economy. The state is near the Brahmaputra River and shares borders with Bhutan, Bangladesh, and Myanmar. Because of its geographical location, this region encounters significant rainfall, especially during the monsoon season, with daily precipitation reaching as high as 250 to 300 mm (10 to 12 inches).A daytime temperature reaches to around 36 °C (96.8 °F), fostering greenhouse-like conditions of intense humidity and heat. The unique malty flavor for which Assam tea is renowned is shaped by the pivotal role played by its tropical climate.

Introduction

The establishment and nurturing of Small and Medium Enterprises (SME’s) is a vital input in creating dynamic market economies in the economic and social development of transition countries. Globalization has created a new world order in which entrepreneurship and systematic innovation play important roles by offering competitive advantage for much needed sustainability of SME’s. To keep up the momentum, the development process of innovative ideas into products require alignment with the changing business paradigms, market dynamics and prevailing economic scenario.

The concept of business incubation dates back to 1950s and was more of providing a real estate facility to the small scale units to tide over the early years’ resource deficiencies. These are called the first generation incubators, which were first established in United States. Later on, the idea of business incubation diffused over different countries, and by the mid of 80s the incubators started providing services like business supports, accelerating the learning curve, including knowledge based services. These are termed as second generation incubators. The third generation of incubators provides the additional facility of networking also to the incubated companies. The importance of networking led to the establishment of virtual incubators or networked incubators, where physical infrastructure has less sanctity. Business accelerators appeared in the mid 2000s, and by now the concept of business incubation is spread all over the world. There exist almost 9000 business incubators in the world.

Seed treatment is one of the important activities of the shop floor. The seed should be treated with various insecticides, fungicides and additives to protect it up to establishment. Some timesenhance quality parameters like vigour which is critical for better establishment. Since vegetable seeds are of very high value and low volume, investment on seed treatment will yield better not only for the seed seller but to the grower also. More number of plants in a field brings moreincome per unit area. Hence the farmer or grower who buys seed is ready to bear the cost of seed treatment making it an important aspect of seed processing.

Seed treatment refers to the application of fungicide, insecticide or both to the seeds to disinfect(deep seated) and disinfest (over seed coat) them from seed borne or soil borne pathogenic organisms and storage insects. It also refers treating seed for enhancing quality by subjecting the seed to solar energy exposure or immersion in hot water or dry heat treatment or priming also.

A big layer of air called the atmosphere surrounds the earth. The air within this layer moves from place to place when it warms up or cools down. This moving air is known as wind. In other words, air in motion is called wind. Winds move moisture and heat around the world and also produce much of our weather.

The strength of the wind can vary enormously. Sometimes air moves slowly and the wind is barely noticeable. When the weather is clear there may be a gentle breeze. When the wind is still very light, but it can be felt and there may be rustlings by leaves. At other times, the air can move very quickly and become a gale or hurricane, blowing down trees and damaging electric poles and buildings.

Winds are part of a global air circulation system that acts to balance temperature and pressure around the world. Different parts of the world receive different amounts of heat from the sun. This differential heating in turn results in differences in temperature and air pressure around the world, which drives the world’s winds.

As equatorial areas are heated most, the air above them warms and rises as it becomes lighter than the surrounding air, causing an area of low pressure. In cooler areas, the air sinks because it is heavier and results in an area of high pressure. Winds will blow as air is squashed out by the sinking cold air and drawn in under the rising warm air. Any difference in temperature like this will always cause a difference in air pressure, and therefore winds will blow. These movements result in a global wind pattern, with air moving between different areas around the world and also at different heights in the atmosphere. Colder air from the poles tends to sink and move towards the equator closer to the surface of the earth. In contrast, warm air from the equator rises and moves towards the poles high in the atmosphere because it is lighter.

Clonal crops belong to at least 34 families including herbs, shrubs, trees and vines. Clonal crop improvement dates back to 10,000 years ago. The diversity of clonal crops and their wild relatives are very significant but not yet fully understood and exploited. Many crops represent combination of sexual and clonal reproduction. Different parts of the clonal crops which may or may not be the same as consumed parts have been shaped as clonal propagules such as stem, setts, tuber, rhizome, corms and bulbs among others.

This chapter introduces the principles and practices required for developing export-oriented floriculture in India. It examines quality standards, grading, packaging, postharvest handling, cold-chain requirements, international markets, export regulations and major trade constraints. The chapter discusses APEDA support, infrastructure, airfreight, cold storage, quality control, planting material and government programmes. It emphasizes the need for internationally acceptable quality standards, efficient supply chains and improved infrastructure to strengthen India's position in the global flower trade.