eChapters

Introduction

Flowering plants (Angiosperms) are identified by their morphological characteristics like flower, fruit and seeds that help their taxonomic characterization into different species, genera, families and orders. The floral characters which are used for taxonomic characterization of plants include the type of inflorescences, perianth structure, floral symmetry, arrangement of floral leaves, type of androecium, stamens, gynoecium, carpel, ovule, etc. The type of fruit and seed also play important roles in taxonomic classification. In this chapter we will briefly discuss about various morphological features of flowering plants (in botanical terms) that are useful in taxonomy.

Nomenclature of Plant

Nomenclature of plant is the formal and scientific naming of plants. It is related to but distinct from taxonomy for grouping and classifying plants and provide botanical name.

The scientific/botanical nomenclature started with Species Plantarum by Carolus Linnaeus in 1753. Botanical nomenclature is governed by the International Code of Nomenclature for algae, fungi, and plants (ICN), which replaces the International Code of Botanical Nomenclature (ICBN).

ICBN and ICN

International Code of Botanical Nomenclature, (ICBN) was adopted by the VIIth International Botanical Congress held in Stockholm, July1950. While the International Code of Nomenclature for algae, fungi, and plants (ICN) was adopted by the XVIIIth International Botanical Congress held in Melbourne, Australia in July 2011.

The response of any living cell to a xenobiotic (including antibacterial agent) is significantly affected by the prevailing local environmental conditions. In case of bacterial infection, it may include the presence of other microbes, no matter commensal or pathogenic, or biochemical alterations at that particular site of bacterial infection in host. All these host and bacterial factors play a critical role in the evolutionary responses of bacteria but usually go unconsidered while determining the microbial sensitivity to a particular antibiotic in a clinical setting. These also include the direct and indirect interspecies interactions that can affect the responses of individual bacterial species and surviving microbial communities to a particular antibiotic therapy. These interactions may significantly alter the qualitative and quantitative responses, potential phenotypic changes and the final trajectory of evolution of antimicrobial resistance. Due acknowledgement and plausible interpretation to these interactions may help to reduce the upsurge in AMR and its associated morbidity and fatality and pave path to more proficient clinical success.

The factors participating in drug- microbe interaction can be classified into two broad groups: host factors and microbial factors. The environmental milieu of the interaction is chiefly regulated by the gut microbiota with stressors causing deflections. The gastrointestinal bacteria amend host mental and physiological cadence via microbial metabolites such as butyrate, polyphenolic antioxidants, vitamins, and amines, affecting the overall ready perception and active rebuke to a stimulus. Lifestyle stressors such as fluctuating sleep and erratic eating patterns (western diet) disturb the host circadian system along with the gut microbiome. The overall impact is impaired detoxification (decreased conjugation of bile acids or increased production of hydrogen sulphide), substrate oxidation and energy regulation (reduced butyrate production) in the host affecting the overall health and productivity.

Introduction
Reproduction involves the intricate interplay of physiological processes to ensure the successful growth and development of the embryo and fetus. Three key processes— implantation, placentation, and the establishment of fetal circulation—are fundamental to a viable pregnancy. These stages ensure the fetus receives adequate nutrients, oxygen, and support for development. Below is a detailed elaboration of each process.
1. Implantation
Implantation is the process by which the blastocyst (a hollow structure formed in the early development of mammals) embeds itself into the uterine wall. It is the first critical step in establishing a pregnancy.
Phases of Implantation
1. Apposition: The blastocyst loosely attaches to the endometrium (the inner lining of the uterus).
2. Adhesion: The trophoblast cells (outer layer of the blastocyst) firmly attach to the uterine lining.
3. Invasion: The trophoblast differentiates into two layers: Cytotrophoblast: The inner cellular layer that serves as the proliferative zone. Syncytiotrophoblast: The outer multinucleated layer that invades the endometrium, facilitating deeper embedding.
Significance
Implantation typically occurs 6–10 days after fertilization.
It ensures the establishment of an early connection between the embryo and maternal blood supply.
Successful implantation depends on hormonal regulation, especially the roles of estrogen and progesterone, which prepare the endometrium to be receptive.

Transformation of data is useful when the assumptions required for analysis of variance are not adequately satisfied. Forestry experiments may produce observations showing skewness, unequal variances, non-normality, or departures from additivity. This chapter explains the need for transforming observations to an appropriate scale before statistical analysis. It discusses the characteristics of an ideal transformation, including stabilisation of variance, improvement of normality, efficient estimation of means, and achievement of linear and additive effects. Various transformation techniques used in forestry experimental analysis are introduced with suitable examples. The chapter emphasizes that appropriate transformation can improve the validity, sensitivity, and interpretability of statistical tests.

A cell culture laboratory should allow the sterile handling of cultured cells with no or minimum level of contamination. The growth of bacteria and fungi is very high and no level of contamination can be allowed in animal cell culture. However, it is impractical to design a laboratory free from potentially contaminated microorganism. Further, it is possible to establish a laboratory with minimal risk of contamination. Several points should be taken into consideration while designing a new cell culture laboratory.

INTRODUCTION

Biological control involves the suppression of pests/organisms with other organisms.  It is an eco-friendly method for management of plant pathogens which can be done exploiting the fungi (Ampelomyces, Trichoderma, Coniothyrium, Candida etc.) or bacteria (Agrobacterium, Bacillus, Pseudomonas and Streptomyces) from rhizosphere,  as well as phylloplane and endophytes (Jayaraman and Verma, 2002). The rhizosphere microfloras are predominantly composed of gram negative pseudomonads.  The fluorescent pseudomonads constitute a large proportion of the rhizosphere microflora presumably due to their nutritional variability being able to use a large number of organic substances (Stolp and Gadkari, 1981). However earlier workers used fluorescent pseudomonads extensively they now prefer Bacillus sp. due to their spore forming ability and heat tolerance which make its formulation easier.

Introduction

We come across a variety of chemicals every day in a laboratory. While some of them are relatively safer, others are extremely hazardous. Thus, it is very important to know the potential hazards that can be caused by the chemicals present in the laboratory and the safety measures needed to be followed to avoid the hazards. Additionally, we should also be aware of the steps necessary to be taken on such hazardous situations. According to IUPAC, a hazardous chemical mean the chemical which has the potential to cause damage in various ways. In a laboratory, there are chemicals that might be irritant, carcinogen, respiratory fibrogen, systemic poison, asphyxiant, flammable, etc. which have the potential to cause hazard to the workers or environment. Therefore, it is important to use appropriate procedure to reduce the risk and protect the health/safety of laboratory personnel, the public and the environment.

Types of Hazards Associated with Chemicals

Chemical hazards: The hazards caused by chemicals can be broadly classified into three categories.

1. Health hazard: This is also known as toxic effects of chemical. The risk associated with a chemical in laboratory depends on the extent of exposure and the inherent toxicity of the chemical. Many chemical reactions form the product that might be much more toxic than the reacting chemicals. For example, inadvertent mixing of formaldehyde (a common tissue fixative) and hydrogen chloride results in the generation of bis(chloromethyl) ether, a potent human carcinogen. For most of the chemicals, the route of exposure (through skin, eyes, gastrointestinal tract, or respiratory tract) is more important (Figure 17.1) that must be considered during risk assessment.

1.     Centrifuge
    
2.     Spectrophotometer
    
3.     Microscope

Abstract

Modern agriculture largely depends on use of high cost inputs and energy intensive farm machinery. Worlds increasing population and continuously deteriorating soil fertility has alarmed agriculture system to look for some safe alternatives. Organic manures are rich source ofnutrients and humans, therefore crop residue recycling by composting is a best alternate of modern high cost input based agriculture. Compost is an aerobically decomposed organic matter .Bacteria, fungi, actinomycetes algae and protozoa are involved in the process of composting and are found throughout the natural environment. The composting material warms up due to microbial activity and it’s temperature becomes up to 160 ⁰F active stage of microbial activity . There are four types of compositing i.e. Windrow composting, Aerated static pile composting, In-Vessel composting and Vermi-composting. The C: N ratio, aeration, temperature and moisture are major factors affecting composting process. Major advantages of composting are: Aggregate formation, Reduction in bulk density, Increase in hydraulic conductivity, Reduction in loss of soil moisture, Increase in organic carbon content and pH of soil.

Abstract

Climate models predict a global average increase of air temperatures in the range of 14^°C by 2100 (IPCC 2014) - a pace exceeding that of past climatic modifications. Change in temperature is expected to influence species occurrence and distribution with up to 37% of all extant species “committed to extinction” due to climate change. These predictions, however, do not include species interactions and evolutionary change which will significantly alter the fate of species. It is therefore important to improve our understanding of host-parasite interactions and subsequent evolution in response to environmental changes in general and temperature changes in particular. Globally livestock production systems are estimated to account for around 18% of emissions. The need of hour is to draw on recent advances in climatology and epidemiology of various parasitic diseases to consider how environmental changes linked with urbanization and climate change can alter the biology of hosts, pathogens and vectors. Climate change is leading to unusual flare-up of diseases, outbreak recurrences, range expansions, or invasion into novel territories and ecosystems, concomitantly with vector or host range shifts. Higher temperatures increase the invertebrate metabolic rate, egg production amount, and feeding frequency, reducing the duration of the development periods and impacting on the number of generations per year and population abundance effecting livestock and human health and socio-economic transformation

Q.1 According to prof. R. A. Fisher “ANOVA” is not the separation of variance assignable to one group of causes from the variance assignable to another group of causes.

Q.2 The basic purpose of ANOVA technique is to test the homogeneity (equality) of several means.

Crop improvement is a continuous process. Crop improvement aims to genetically enhance, enrich and empower the crop plants with superior performance, enhanced yield level, higher quality of food, nutritional, fodder, energy produce and adaptability to climate change and crop growing environments. The intrinsic virtues of the improved cultivars could be sustained mainly through the production of the quality assured seeds endowed with genetic capabilities. The quality assured seed delivery system is through the seed chain from the basic seeds through Nucleus-Breeder-Foundation-Certified seed. Quality seed production system need to be based on genetic, breeding and seed technological protocols including field and laboratory testing to ensure very high genetic purity, true to type and superiority.

Major industries constituting the food processing industry are grains, sugar, edible oils, beverages and dairy products. The key sub-segments of the food processing industry in India are dairy, fruits and vegetables, poultry and meat processing, fisheries, food retail etc. Agro processing is the sunrise sector of the Indian economy in view of its large potential for growth and likely socio economic impact specifically on employment and income generation. India has the advantage of being one of the fastest growing economies in the world and it is the largest producer of several agricultural commodities. The food processing sector ranks first in terms of employment and number of factories in operation and third in terms of output.

In India, out of the total production of fruits and vegetables, nearly 76 per cent is consumed in fresh form, while wastage due to improper post harvest accounts for 20 to 35 per cent. Only around 2 percent of vegetables and 4 percent of fruit production are being processed. 

Theoritical aspects of design has been discussed in chapter 1. Discharging capacity of canal is

 computed by Manning Equation (1)and continuity Equation (2) (Para 1.4).

 In the Manning's equation  V  =   R2/3  S1/2 / N (Eq. 1, Para 1.4);  N and R play most important role.

 The effect of  R has been discussed in para 1.4.1, Chapter 1. In this chapter the complexities of Manning's n (or N, also known as Manning's rougosity coefficient

 or simply rougosity coefficient) and some practical aspects of composite sections and velocity in best

 Mehboob, trapezoidal or rectangular sectioned channels are discussed.

2.0 Manning's Rugosity Coefficient (n)

Value of N depends upon a number of factors such as , surface roughness, vegetation, obstructions, type of lining, age of lining, erosion and damages in lining, curves, discharging capacity , perimeters, depth of flow, velocity etc. These are discussed below and commonly adopted values of n are given in tables 2.1 to 2.3.

Introduction

Proper electrical wiring and earthing are crucial for laboratory safety and functionality. A laboratory houses several installations/equipment that demand stable/continuous power supply. Inappropriate or faulty wiring and earthing may lead to significant risks, including electrical shocks, damage to equipment, and even incidence of fire. Therefore, understanding the requirements of electrical wiring and earthing is essential for designing, construction, and maintenance of laboratory. This chapter aims to provide a basic knowledge of electrical wiring and earthing necessary for a laboratory. Importance of earthing in mitigating electrical hazards and ensuring integrity of electrical installations are also described.

Basics of Electrical Wiring

Basic understanding of electrical wiring is necessary for laboratory safety and efficient electrical functioning. The potential difference between two electrical points (negative and positive) is known as Voltage, which drives the flow of electric current. This is measured as volt (V). The rate of flow of electricity in a circuit is known as Current (I), which is measured as ampere (A). Current can be either direct (DC) or alternating (AC). Opposition/resistance experienced in the flow of current in circuit is known as Resistance (R) and it is measured in ohms (Ω). Higher the resistance means lesser the current flows at a given voltage. The rate at which electrical energy is transferred/utilized in an electric circuit is known as Power (P), which is measured in watt (W). The Power can be calculated as the product of voltage and current (P = V × I).

Abstract

As such in search of alternative to chemical insecticides against insect pest of rice resulted in identifying white muscardine fungus, Beauveria bassiana an entomopathogen out of several entomogenous fungi prevalent in rice ecosystem as the potential mycoinsecticides. The fungus has 78-87% virulence against rice hispa with  LC 50 value of 90.16. Under field situations it is required that the effectiveness of the bioagents are enhanced by combining them with compatible pesticides. A sublethal dose of a pesticide  would make the insect physiologically weak which makes it much more susceptible to the attack of the entomogenous organisms. In view of this, compatibility test was conducted with 14 different commonly applied insecticides at two different doses i.e., recommended and half of the recommended dose. In small scale field trial chloropyriphos at half of the recommended dose along with B. bassiana was the best treatment in controlling the pest along with increased yield of the crop consecutively for two years.

Abstract

The future food grain demand in India is projected to increase beyond 300 million tons by 2025. There are only two ways to meet the increasing food and other agricultural demands of the country’s rising population either expanding the net area under cultivation or intensifying cropping over the existing area. The net sown area of the country has risen by about 20 per cent since independence and has reached a point where it is not possible to make any appreciable increase. The option left is go for high cropping intensity for ahead of present of 137%. A major share of this increase is likely to come from increasing the number of crops produced per year on a given land using improved crop cultivars. Research indicates that maximum intensity of 400-500% is possible provided situation fulfills the requisite factors such as irrigation facilities, short duration varieties, mechanization and agrochemicals are available. The objective of increasing the cropping intensity will be achieved only by the inclusion of short duration varieties of cereals, identification of pulses and oilseed crops that can fit well in the cropping systems of different agro-ecologies that will ultimately improve crop productivity per unit of land making the efficient utilization of resources and will meet the objectives of crop diversification leading to food and economic security of the nation.

Completely Randomized Design (CRD)

The simplest design using the two essential principles of replication and randomization is the CRD. Suppose that we have t treatments (or t levels of a factor) under comparison and ith treatment is to be replicated r times, for i = 1,2, … t . A particular case of this is equal replication for different treatments, where r1=r2= =r6=r, so that n=rt.