eChapters

Mode of reproduction
All the living organisms produce individuls of their own kind for the survival of their species which is called reproduction. Hence, reproduction is the biological process by which new individual organism (offspring) are produced from their “parents”. In vegetable crops, modes of reproduction may be sexual, asexual and both. Most of the vegetable crops viz., tomato, brinjal, chilli, sweet pepper, peas, cowpea, hyacinth bean, French bean, cluster bean, runner bean, fenugreek, lettuce, cabbage, cauliflower, knolkhol, Brussels sprouts, sprouting broccoli, radish, beet, turnip, carrot, onion, leek, amaranth, palak, spinach, asparagus, pumpkin, bottle gourd, watermelon, muskmelon, ridge gourd, snake gourd, wax gourd, summer squash, bitter gourd, cucumber, sweet corn, coriander, parsnip, etc. are exclusively propagated sexually for both commercial cultivation and breeding purpose. Some vegetable crops like, pointed gourd, teasle gourd, yam, taro, Jerusalem artichoke, globe artichoke, etc. are exclusively propagated through asexual mode of reproduction. On the other hand, some vegetable crops like, potato, sweet potato, cassava, annual moringa, etc. can be propagated by both sexual and asexual modes of reproduction however, they are generally propagated asexually for commercial cultivation and their sexual reproduction is employed for the purpose of breeding.

Introduction
The advent of antibiotics, beginning with penicillin in 1941, transformed medicine by rendering bacterial infections treatable [1]. However, antimicrobial resistance (AMR) has emerged as a profound threat, enabling bacteria to survive in the presence of drugs designed to inhibit or kill them and these are rooted in ancient microbial ecosystems evidenced by resistome genes in 30,000-year-old permafrost [2]. AMR has been exacerbated by human practices: approximately 50% of antibiotic prescriptions are inappropriate, 70% of global antibiotics fuel agriculture, and environmental pollution creates resistance hotspots [1]. By 2025, AMR directly causes 1.3 million deaths annually, with economic losses projected at $100 trillion by 2050 [1]. Priority pathogens, including carbapenem-resistant Enterobacteriaceae (CRE), methicillin-resistant Staphylococcus aureus (MRSA), and colistin-resistant strains via mcr-1, challenge last-resort therapies, particularly in low-resource settings [3]. This chapter delivers a technically extraordinary exploration of AMR, structured into genetic, biochemical, and evolutionary mechanisms, each elaborated with molecular precision and step-by-step clarity for learners. Genetic alterations provide the DNA-level foundation, biochemical pathways execute molecular defenses, and evolutionary forces drive adaptation and dissemination. It ensures scientific rigor and broad appeal for students, researchers, clinicians, and policymakers.
Historical Context and Global Burden
AMR emerged shortly after antibiotics’ introduction, with penicillin-resistant S. aureus identified by 1942 due to ß-lactamase production [1]. The 1960s

Azospirillum is a spiral-shaped nitrogen fixing bacterium. It also produces hormones and vitamins. Important species are Azospirillum brasilense and Azospirillum lipoferum. It is widely distributed in soils and grass roots.The genus Azospirillum belongs to family Spirillaceae with four commonly available species viz. A. brasilence, A. lipoferum, A. amazonense and A. halopraeferens or A. seropedicae (Fig. 8.1).

Introduction

The study of infectious diseases, alongside other clinical and natural strengths, is going through fast change introduced on by the approach of reasonable next generation sequencing (NGS) advancements (Koser et al., 2012).These technologies become adopted as routine methods easily, and laboratory techniques are transformed throughout the process. The amount of genomic bacterial data generated is huge. As of this writing, for example, over 200,000 Salmonella genomes alone are in the public domain with hundreds being added weekly. A whole genomic DNA sequence characterizes the maximum practicable level of structural detail on the individuating traits of an microbial organism or population. It can therefore be used to provide more accurate microbial genome recognition, evolutionary relations and a detailed catalogue of epidemiologically important characteristics. This has a significant role to play for outbreak research, diagnosis and treatment of infectious diseases, microbiology and epidemiology (Allard et al., 2019). Besides, DNA sequences are also a universal dataset which can theoretically be used to determine any biological function. This means that antimicrobial resistant (AMR) can be detected and to track the development and spread of AMR bacteria in a hospital or the community.

Introduction

Introduction to Feasibility Reports

A feasibility report is a detailed analysis of the viability, sustainability, and profitability of a proposed project. It is crucial for decision-making and project planning, particularly in the dairy industry where multiple variables affect the outcomes.

Objectives of Feasibility Reports • Assess economic viability.

• Analyse technical requirements.

• Evaluate operational sustainability.

• Provide a roadmap for project implementation.

• Decision-Making Tool: Helps stakeholders decide whether to proceed with a project. Hence, minimize risks through identification of potential obstacles and informed decision-making.

• Resource Optimization: Ensures efficient use of resources like time, money, and labour.

• Stakeholder Communication: Provides a structured overview of the project’s potential.

Carbohydrates (glucose) are the primary energy source for a body; when digested, glucose is the end product in human beings and simple stomach animals, but it yields volatile fatty acids in ruminants. Precise regulation of levels of blood sugars/glucose in the body is the net result of various processes like digestion, absorption, and metabolism operating under the interaction of various a) enzymes and b) hormones secreted by the different organs of the body. An abnormal secretion of any of the enzymes or hormones associated with the metabolism of carbohydrates disrupts the precise regulation of blood sugar, leading to various disorders.
14.1 Enzyme Deficiency and Disorders in Carbohydrate Metabolism
Usually, various enzymes are involved in the breakdown of carbohydrates, and if their secretion is insufficient, missing, or does not work correctly, it leads to different disorders.
I. Glycogen storage diseases (GSDs, glycogenosis, and dextrinosis)
Glucose is needed by the cells for the generation of energy and other cellular activities. If glucose happens to be in excess, it is stored as glycogen, mainly in the liver and muscles, for future needs. The stored glycogen can be converted back to glucose when the need arises. Many enzymes are related to this interconversion of glucose and glycogen. Deficiencies of one or more enzymes may lead to abnormal glycogen metabolism, leading to glycogen accumulation of normal or abnormal chemical structure in tissue, collectively called glycogen storage diseases (GSDs). Roman numerals identify different types of glycogen storage conditions. They are also classified under various categories like inherited metabolic diseases, autosomal recessive type, and X-linked type (VIII/IX type).

A system of housing is more are less synonymous to the system of management of livestock. Housing is essential to protect the animals from adverse climatic condition (rains, hot and chilly winds). Housing also help to maintain normal health of animals by proper feeding watering and reducing environmental stresses.  Housing allows the animals for proper rest, moderate exercise, suitable micro-environment and proper ventilation which are essential for comfort of the animals. While selecting the site a site for establishment of a dairy farm some points should be considered regarding topography, soil, nearby market, availability of labour, electricity, water etc. The housing system can be divided in to two type viz. conventional housing and loose housing system. The conventional housing system may be single row or double row depending upon the number of animals. The dairy farm consists of various building for different classes of livestock like calf shed/ lamb/kid/piglet pens, milking barn, maternity pen, cow shed, heifer shed, shearing room, slaughter house of pig etc. The ancillary structure of  a farm  are stores for keeping feed materials, equipment, milk house, silo, generator room, pump houses, AI centers, workers retiring room, office etc. The floor should be impervious and non-slippery. The floor should have a gradient toward the drain of 1 in 40 in covered area and 1in 60 in the open area for easy drainage of urine and washing of animals. The roof should be light, strong

Split-plot design is particularly useful when certain biological treatments cannot conveniently be applied to small experimental units. This chapter explains how large experimental plots can be divided into smaller sub-plots to accommodate subsidiary treatments. Main-plot treatments are randomly allocated to larger units, while sub-plot treatments are independently allocated within them. The design allows researchers to study main effects, sub-plot effects, and interactions, although greater precision is generally obtained for sub-plot comparisons than for main-plot comparisons. Examples include wood preservation and nursery experiments involving sowing methods and seasons. The chapter also explains the two error terms required for appropriate analysis of split-plot experiments.

Over the past several years, there has been significant change in consumer perception on quality and food safety. Consumers are demanding healthy, nutritious and cost-effective products processed under controlled hygienic conditions. Food safety concern, in recent years, has become dominant and important. The requirement of food safety and quality assurance has never been so demanding as it is now. Globally food safety is being addressed independent of food quality. In anticipation of consumer demands on food safety, food sanitary standards at national and international levels are under constant review. Several inter governmental agencies / organizations are engaged in work related to quality assurance and food safety. Amongst these World Health Organization (WHO) and Codex Alimentarius Commission (CAC) play the major role in protecting public health and defining standards of quality and safety aspects of foods for regional and or world wide usage.

Regulatory Institutions

The quality environment in the Indian food processing sector revolves around compulsory legislation which specify minimum standard and certification system. Legal standards are generally made to have control over the quality of foods offered for sale and to safeguard the consumer from health hazards posed by practice of adulteration. There are basically three types of Indian standards namely

Soil texture and mechanical analysis: The chemistry of the mineral framework was explained in Chapter II. Its physical properties are quite as important- When all the extraneous matter is removed from the soil we are left with a collection of particles of various sizes. These particles are not spherical in shape, but most of them are nearly so. At any rate the discussion is very much simplified if we suppose that they are spherical and the arguments advanced are equally applicable if the shape deviates from the spherical.

India has a population of one hundred twenty four crore plus and feeding this large a population calls for a strong FPI. The industry also assumes importance in the national economy due to the immense employment generation opportunities it offers and the export revenue it earns. Food Processing Industry (FPI) in India is a sunrise sector that has gained prominence in the recent years. Easy availability of raw materials, changing lifestyles and favourable fiscal policies has given a considerable push to the industry’s growth. FPI serves as a vital link between the agriculture and manufacturing sectors of the economy. Strengthening this link is critical to reduce wastage of agricultural raw materials, improve the value of agricultural produce by increasing shelf-life as well as by fortifying the nutritive value of the food products and ensure remunerative prices to farmers as well as affordable prices to consumers. The industry has very low processing level i.e., 2.2% for fruits and vegetables, around 35% in milk, 21% in meat and 6% in poultry products, which is significantly lower by international standards. For e.g., processing of agriculture produce is around 40% in China, 30% in Thailand, 70% in Brazil, 78% in the Philippines and 80% in Malaysia. Value addition to agriculture produce in India is just 20% with wastage estimated to be valued at around US $13 billion.

India has the second largest producer of food with arable land of 159.65 million hectares and has the highest acreage under irrigation. Next to China, India ranks second largest food producer in the world and has the potential to immerge the biggest with its food and agricultural sector. India accounts for less than 1.5% of international food trade despite being one of the world’s major food producers, which indicates huge potential for both investors and exporters. Food and food products are the biggest consumption category in India

The process of fracture healing is unique, in which damaged bone restores its original architecture but it does not form a poorly organized replacement matrix, otherwise known as scar tissue, rather it regenerates the original matrix and retains its mechanical properties. In order to repair bone defects, a combination of cells with osteogenic activity and appropriate scaffolding material can stimulate bone regeneration and its repair. A potential substitute for autologous transplantation should possess 3 elements: osteoprogenitor cells, osteoinductive factors, and an osteoconductive scaffold. Stem cells from adult tissues are attractive materials for cell therapy and tissue engineering. These cells generally have restricted lineage potential when compared to embryonic stem cells, and this may be advantageous in certain therapeutic applications. Bone-marrow-derived mesenchymal stem cells (BMSCs) have proven to be beneficial in bone regeneration. Bone marrow stem cells are pluripotent cells with the capability of differentiating osteoblasts so that they have been used to facilitate bone repair. Several studies have shown that seeding of cultured BMSCs on bioabsorbable implants can induce bone formation in vivo and lead to improved healing of critical-size bone defects.

Mesenchymal stem cells (MSCs) because of their self replication and osteogenic differentiation capabilities are regarded as an excellent source of cells for bone tissue engineering. Autologous stem cells are not always preferable since the quality and quantity of such cells will be affected by metabolic diseases, old age, and osteoporosis. Allogenic BMSCs might be preferable to xenogenic, but they are always not readily available. In addition, allogenic MSCs have the potential for carrying some diseases. The use of non-autologous stem cells isolated from healthy donors offers a major advantage since these stem cells can be thoroughly tested and formulated into off-the-shelf medicine in advance. Major attractive advantage of BMSCs as a source of cell transplantation is their low immunogenecity. It is now well established that BMSCs are immune-privileged cells that do not elicit immune responses due to an absence of their immunologically relevant cell surface markers.

Series of experiments are conducted at different locations, times, or combinations of locations and years to evaluate the spatial and temporal performance of treatments. Such experiments are especially important in forestry and agricultural research when conclusions are intended to apply across wider geographical or environmental conditions. This chapter explains combined analysis of a series of experiments after individual experiments have first been analysed according to their respective designs. Particular attention is given to testing the homogeneity of error variances, including Bartlett’s test. When variances are homogeneous, pooled analysis can assess treatment effects, location or year effects, and treatment-by-location or treatment-by-year interactions, thereby evaluating consistency of treatment responses.

Abstract 

The on-farm programme on SRI was carried out in the village Narayanpur under RLI command area in Karimpur II Block of Nadia district in the year 2016 during summer season. The soil of farmland is clay loam and situated having latitude of 23°54’26.5"Nlongitude 88°30’24.1"Eat an average elevation of 9.75 m above the mean sea level. Monsoon ceases during October and cool season sets in by November. The average rainfall of this region is about 1500 mm and normal monsoon breaks in the first week of June. Though water resources in this area are generally satisfactory and 90% of these resources are tapped form the ground water source through deep and shallow tube wells. However, due to faulty crop planning and lack of scientific water management approach, a significant portion of agricultural area is identified as gray zone from hydrological point of view. Even many tube wells are becoming dry due to over exploitation of valuable water from the lower depths of confined aquifers. Rice is predominant crop during kharif season becoming less profitable because of high input costs involved. Summer rice gave satisfactory yield but also profitable proposition because of more input cost and less farm gate prices.  

Dogs, Cats and birds are pet animals.

Chromosome number of dogs-78

Good Angora breed yield wool of 2-2 ½ “ an average. On record, 8” of wool is also obtained.

Nitrogen is a nutrient and occurs in many forms including ammonia, organic, nitrate and nitriteeach of which may be tested for in a variety of ways. Under normal circumstances, the nitrite form of nitrogen will not be present in large quantities due to its rapid oxidation orconversion to nitrate. The presence of large concentrationsof ammonia in a stream or lake can create a large oxygen demand. This demand is caused by the conversion of ammonia to nitrate. High concentrations of nitrate in waste water treatment plant effluent can cause algae to grow in large quantities.

Current status

For many years, the protein contentof foods has been determined on the basis of total nitrogen content, while the Kjeldahl (or similar) method has beenalmost universally applied to determine nitrogen content (AOAC, 2000).Nitrogen content is then multiplied by a factor to arrive at protein content. This approach is based on two assumptions: thatdietary carbohydratesand fats do not contain nitrogen, and thatnearly allof the nitrogen in the dietis present as amino acids in proteins. On the basis of early determinations, the average nitrogen (N) content of proteins was found to be about 16 per cent, which led to use of the calculation Nx6.25(1/0.16 = 6.25) to convertnitrogen contentinto protein content.

Despite the benefits that minimally invasive surgery and osteosynthesis have brought to fracture management and bone healing, there are still many circumstances where bone healing remains challenging. Large bone defects are serious complications that are most commonly caused by extensive trauma, tumour, infection, or congenital musculoskeletal disorders. In nonunion cases repairing of bone defects with composite biomaterials as defect filler can promote bone regeneration. Currently, the gold standard for bone regeneration is use of autogenous bone graft. In order to avoid morbidity at the donor site or if large amount of autogenous bone is needed, bone substitution materials can be used. Bone substitution materials can be combined with cells such as mesenchymal stem cells (MSCs) to increase bone formation. Bone-marrow derived mesenchymal stem cells (BMSCs) represent an attractive cell population for tissue regeneration. Bone marrow stem cells are pluripotent cells that have been used to facilitate bone repair because of their capability of differentiating into osteoblasts. Several studies on the regeneration of bone have shown that cultured BMSCs, seeded on different bioabsorbable implants are able to induce bone formation in vivo and lead to improved healing of critical-size defects.

Calcium phosphate bone substitutes such as hydroxyapatite (HA) and tricalcium phosphate (TCP) are currently used for bone substitution in many different clinical applications such as repair of bone defects after trauma or tumour and bone augmentation in spinal arthrodesis. Although these bone substitutes are osteoconductive, they often lack the osteogenecity needed to support bone healing in large defects and are slowly degraded in the body. Studies have shown that coating of hydroxyapatite with a calcium silicate layer could encourage cell proliferation and osteogenic differentiation of human bone marrow-derived stromal cells. Silica-calcium phosphate composite in comparison to calcium phosphate-rich biomaterials has a faster resorption rate owing to greater dissolution of Si ions. The use of several growth factors have been studied in bone repair and these factors are known to play a role in differentiation of mesenchymal progenitor cells specific lineages like endothelial cells or osteoblast. Bone morphogenetic protein-2 (BMP-2) has been shown to accelerate bone healing in humans and animal models. BMP-2 acts by osteoinduction and is involved in the differentiation of mesenchymal progenitor cells into osteoblasts. The addition of recombinant human BMP (rhBMP-2) to a self-cross linkable cellulose hydrogels/biphasic calcium phosphate granules construct promotes bone regeneration in a critical-size segmental defect model of non-union in dogs. It is also reported that protein, insulin-like growth factor (IGF-1) stimulates direct migration of human mesenchymal progenitor cells (MPC) and contributes to the recruitment of MPC in bone formation and bone healing.

In the global scenario, the increasing energy crisis and consequent escalation of chemical fertilizer prices along with increasing risk associated with soil degradation have reviewed interest in bio-resources for attaining soil health and sustainable crop production. The use of organic manures, bio-fertilizers and chemical fertilizers will augment the efficiency of combined application of nutrients to maintain a high level of soil productivity and rice production (Thakuria et al., 1991; Prasad et al., 1995). Several experimental evidences showed that the combination of various bio-resources with chemical fertilizers improved the rice production in sustainable way (Roy and Jha, 1987; Subba Rao, 1995; Mahapatra et al., 1997; Suresh et al., 2001).

Communication is a key to understand things between two people. Today’s communication is entirely different from pre-historic era and has taken many evolution throughout the years. Before alphabets, signs, symbols, letters and telephone evolved, communication was restricted to interpersonal interaction (i.e.) from one person to another in the form of gestures. It is in other terms called non-verbal communication. Today, communicating between two or more people has become too easy that one can communicate with more than 10 members at a time which is in technical means called conferencing.

The growth of internet has paved way for innumerable means of communication. In other words it can be told as, communication is re-defined by technology. Texts, email, tweets, tele and video conferencing have widely saved much time of people and made them recipients of today’s fast moving world.

Communication beyond history

Ancient nomads used to communicate with the help of drawings and paintings. Chauvat Cave in France is one such example which proves that communication through cave painting had taken place 30,000 years ago Before Christ (BC). Throughout the world many such cave painting monuments has been discovered. One in South Sulawesi, Indonesia and Coliboaia Cave in Romania gives an idea about how communication would have prevailed among people those days.

Later came symbols. Historical evidences prove that, symbols were used by people around 10,000 B.C. Through rock carvings pictograms were developed by ancient people to tell a story to their next generations through logographic images. It is said that, culture among human groups have evolved through rock carvings. Later, ancient cultures developed ideograms. Egyptians had their own style of communication called Hieroglyphs. Chinese created their own characters. Lastly, the alphabets re-defined language and communication was developed around 2000 BC.

INTRODUCTION

Aquaculture is a technology driven industry which relies a lot on research to develop species diversification in culture system as well as in the appropriate technology for commercial production. The rapid development of aquaculture in the world depends largely on the availability of fish feed; which are traditionally based on fish meal as the main protein source (Hardy & Tacon, 2002; Krishnankutty, 2005; Yigit et al., 2006) but the major challenge is its availability, quality and cost (Ghazala et al., 2011; Tabinda and Butt, 2012). In the aqua feed formulation, protein is the main but expensive ingredient and its quality and quantity in fish feeds formulation plays a vital role in promoting fish growth (Pandian, 2001). Dietary protein provides essential and non essential amino acids to synthesize body protein and energy for maintenance. According to fish species, fish size, dietary protein sources and environmental conditions protein requirements ranges from 300 to 550 g /kg (NRC 1993).

In India there is a significant development in fresh water aquaculture in last few decades contributing greatly to overall fish production of the country. The fresh water aquaculture is crossing the traditional boundary of concentration on farming of major carp and entered the arena of diversification to the culture of other species like catfishes. So far in India majority of research works were concentrated on development of commercial fish feed mainly for carps and shrimps. To fulfill the objectives of large scale culture of cat fishes and development as an economic proposition, it is necessary to take up induced breeding and larval rearing of commercially important catfishes in controlled conditions and develop easily adoptable package of practices of the same to cater to the needs of interested fish entrepreneurs. Among the catfishes, Clarias magur, an air breathing species and popularly known as ‘magur’ or ‘walking catfish’ is a high valued fish in the Indian subcontinent and fetches higher price than many other fishes including major carps and it is highly revered for its nutritious flesh which is therapeutic in nature, hence, better prospects are there for developing its culture (Hussain et al., 2016). Vishwanath (2010) reported that this species is very much popular in Bangladesh due to use as important part of the diet for children and lactating mothers and also prescribed as diet for the convalescent of the patients. This fish is highly regarded for food due to its high protein (15.0%), low fat (1.0%) and high iron content (710mg/100g tissue). Paul et al. (2015) in their studies on Indian catfish namely magur (Clarias batrachus) and singhi (Heteropneustes fossilis) for proximate composition and mineral content concluded that both the fishes are rich in protein, fat and minerals content irrespective of their size and season.