eChapters

Dry Flower Products

Dry flowers are aesthetically beautiful and they enhance the décor and liveliness of any room. They bring the fiery warmth and bounty of the season into the home with ease every fall. Much of fall’s beauty lies in the glorious changing colors of autumn leaves. These flowers are dried to use them in a wide range of products. Dried floral products are widely used for decoration and for adding zest to a room. Apart from that they are also used as an essential component of many products. They are widely used to make handmade paper, lampshades, candle holders, jute bags, photo frames, boxes, books, wall quilts, topiary, cards and several gifts. The use of dry flowers in the making of these products enhances the appearance and beauty of these products. They look sophisticated when gifted to someone or used as a décor of your own home.

23.1 Dry Flower Packing

Careful packing is one of the most important aspects of any product. Dry flower packing is the simplest way to capture the essence of summer flowers till long. Dry flowers should be packed nicely to avoid any damage. They should be packed alone in appropriate sized carton loosely cushioned with paper.They should be packed in a manner to keep them away from direct sunlight leaving some room for air. Packing material should be stable and firm to hold the dry flowers well without any damage during storage or transportation. Dry flowers can be packed in a variety of ways: in poly bags, window box, cardboard box, or in a sachet.

Driftwood is wood that has been washed onto a shore or beach of a sea or river by the action of winds, tides, waves or man. It is a form of marine debris or tide wrack.

In some waterfront areas, driftwood is a major nuisance. However, the driftwood provides shelter and food for birds, fish and other aquatic species as it floats in the ocean. Gribbles, shipworm sand bacteria decompose the wood and gradually turn it into nutrients that are reintroduced to the food web. Sometimes, the partially decomposed wood washes ashore, where it also shelters birds, plants, and other species. Driftwood can become the foundation for sand dunes.

Preserving plant materials in a dried form is not a new idea. It has been considered as an art for hundreds of years. Fragrant dried herbs were placed in Egyptian pyramids. Dry flower arrangements have been popular in Europe for several centuries. During 1700 A.D, the early American settlers used dry flowers to brighten their homes. Any plant material can be preserved for use as dry flowers (Al Pertuit, 2004). Though enormous work is being done in this field, most of the literature is published by horticulture amateurs. Scientific work in this area is less. However, a review of the research work done in dry flower technology is discussed in detail.

ABSTRACT

Biodiversity is often understood in terms of wide variety of plants, animals and micro-organisms existing on the earth. So far, about 1.75 million species have been identified, mostly small creatures such as insects. According to scientific estimates, there are actually about 13 million species, although range from 3 million to 100 millions. The present article deals with the importance and threats of biodiversity as well as tries to highlights the role of government and non-government sectors in conservation of biodiversity. The initial part of the article indicates the concept of evolution and biodiversity, wherein we have tried to identify the present scenario. We find that a wide range of microbial, plant and animal species are sources of various biological products for human use and welfare. These species are getting lost or extinct due to lack of conservation over a period of long time. Therefore, an effort has been made to highlight the conservation acts and important rules and regulations for protecting and conserving the bio-resources.

ABSTRACT

Microorganisms play a key role for the proper running of the ecosystem. They help in nitrogen fixation, which is unique and most necessary property found only in this group. They help in decompositions, mineralization, nutrient cycling, phosphate solubilization, soil aggregate formation, symbioses, parasitism and many other important works. A great emphasis has been given by many scientists to conserve the microbial diversity. Developing human civilization has a great negative impact not only on the above ground biodiversity but also on the belowground biodiversity. This article is an overview, what important and crucial role nitrogen fixers play for maintaining soil and plant health. Nitrogen fixer not only fixes atmospheric nitrogen but also produces growth promoting hormones which are very important for plant growth. 

Abstract

Mycorrhizal symbiosis is a highly evolved mutually beneficial relationship found between Arbuscular Mycorrhizal Fungi (AMF) and vascular plants. Knowledge of taxonomic and functional diversity of AM fungi is important in understanding the biology and ecology of such a widespread mycorrhizal symbiosis. Classification and identification of AM fungi remains in the formative stages of growth, with all the attendant turmoil. Since Glomus is known to be an important AM genera for providing multifacet benefit to several economically important plants. Hence, in this review article an attempt has been made to describe 120 Glomus species along with the line diagram of 63 Glomus species for their proper identification that can be used as an identification manual for mycorrhizal researchers.

ABSTRACT

Pesticide degradation is the breaking down of toxic pesticides into a non-toxic compounds and, in some cases, down to the original elements from which they were derived. The most common type of degradation is carried out in the soil by microorganisms, especially the fungi and bacteria. Microbial degradation depends not only on the presence of microbes with the appropriate degradative enzymes, but also on a wide range of environmental parameters. Pesticides which are rapidly degraded are called non-persistent while those which resist degradation are termed as recalcitrant (persistent). Microorganisms are able to degrade a large variety of compounds, including pesticides under laboratory conditions. However, methods have yet to be developed to decontaminate the environment from pesticide residues. Continuous efforts are required in this direction, and at present several PGPR capable of degrading pesticides have been isolated from the natural environment. Catabolic genes responsible for the degradation of several xenobiotics, including pesticides, have been identified, isolated, and cloned into various other organisms such as Streptomyces, algae, fungi, etc. In addition, recombinant DNA studies have made it possible to develop DNA probes that are being used to identify microbes from diverse environmental communities with a unique ability to degrade pesticides. Bioremediation treatment still requires more intense research through specific microbial degradation of broad range of organic pollutants. This review describes recent advances in biodegradation of pesticides by addressing the microbes involved in pesticide degradation.

ABSTRACT

The present paper describes application of synergistic action of microalgae viz., Spirulina platensis, Chlorella vulgaris, and Oscillatoria princeps coupled with state-of-the-art microbial biotechnology (that involved strains of Bacillus subtilis, Nitrobacter winogradskyi, Aspergillus flavus, and A. niger) developed by Biotechnology Resource Centre (BRC), Mumbai for treatment of secondary liquid effluents from a gelatine manufacturing industry. The growth of mixed cultures of selected microbial and algal species was measured respectively in terms of Colony Forming Units (CFUs) and Optical density (O.D.) of the culture. The results were indicative of efficient degradation potential of the proteinaceous liquid effluents. Moreover their BOD and COD loads reduced by 30.75 % and 84.10 % respectively after the treatment. Furthermore, the bio-safety of algal biomass cultured in selected effluents as feed supplement was tested on Swiss albino mice (Mus musculus). Mice in treated group didn’t exhibit any observable adverse effect on their growth or on their reproductive performance. In fine, the liquid effluents of gelatine manufacturing industries were polished along with production of probiotic food supplement.

Abstract

Elaboration of mycotoxins by several moulds in different agricultural commodities has been reported to be widespread. The mycotoxins are reported to cause variety of health hazards in different animals including Chicks, cow, horse, rat, Mouse, pig, rabbit, dog, including man. More than 150 species of moulds are reported to elaborate chemically and biologically diverse toxic metabolites. These mycotoxins  are known to induce variety of diseases commonly known as mycotoxicoses.The fungi which elaborate mycotoxins are diverse belonging to different groups of fungi.Mycotoxigenicity of these moulds not only differ among different genera but also among different species.  Some fungi such as Aspergillus flavus, A. terreus, Penicillium griseofulvum, P.expansum, P.nordicum, P.verrucosum, Fusarium acuminatum, F.culmorum, F. graminearum, F.moniliforme, F.oxysporum, F.solani, F. tricinctum and Cheatomium globosum are known to produce more  one  type of mycotoxin which differ in their chemical structure and biological activity.

Abstract

Nematodes are non-segmented worms typically 50 µm in diameter and 1 mm in length. Those few species responsible for plant diseases have received a lot of attention, but far less is known about the majority of the nematode community that plays beneficial roles in soil. Nematode diversity tends to be greatest in ecosystems with the least disturbance. Species of Aphelenchoides, Ditylenchus spp, Helicotylenchus, Meloidogyne spp., Pratylenchus, Scutellonema  and Rotylenchulus are obnoxious plant parasitic nematode beside Heterodera and Globodera. Soil temperature, soil structure, soil pH, soil moisture and soil texture play major role in movement of nematode, infestation and completion of life cycle under abiotic and biotic stresses.

Abstract

Trichoderma species play a vital role in the management of plant diseases in association with different commercial bioformutlations of the fungus. Its role as a successful pollution free environment and friendly mechanism of action has been highly exploited. The diversity of species based on location, type of agri-land and association with different fauna  and flora has been instrumental in large upsurge of biological methods of Plant disease management. Pollution of agri-land soils with chemicals, heavy metals and development of resistance to diseases has become an emerging problem coupled with the high costs of Infrastructure. Thus, the interest in alternative biologically based systems is the need for bioremediation, bioecology and biocontrol with Trichoderma.

ABSTRACT

Decomposition of litter is a dynamic process. It regulates cycling of nutrients in ecosystem. The productivity of an ecosystem depends on the turnover of carbon and other nutrients. Microbes play an important role in releasing of minerals locked up in organic complexes. The release of nutrients, however, is regulated by various climatic factors. The microbial succession at different stages of degradation of litter is controlled by the litter quality and species composition and biological activity. The present paper reviews the status of litter production, decomposition, species diversity and Biological activities as affected by the climatic and edaphic factors in tropical ecosystem.

Keywords : Litter decomposition, microbes, species diversity, biological activities, edaphic factors, tropical ecosystem.

ABSTRACT

Microbes are integral part of the terrestrial ecosystem. Their function ranges from nutrients mineralization, stress alleviation to bioremediation in soils .There is a great possibility of using microbial inoculants as a biological tool for sustainable forestry and agriculture. This review gives an overview on the benefits of microbial technology and their contribution in development of green technology.

Keywords: Green technology, Microbes, AMF, PGPRs, Microbial consortium, Carbon sequestration.

Abstract

Microorganisms are potential tool for the production of large amount of potent and effective health care, food products and biofertilising agents. Recently, the marine microorganisms are considered as good sources of such products which may possess novel properties. There is a high scope for the exploration of marine microbes in order to search new product of economic importance from marine resources. Mangrove habitat is also part of marine habitat with the plant community having unique adaptation in their morpho- physiological and reproductive behavior. The high level of organic content in mangrove rhizosphre also leads to the colonization of degradative microorganisms and make them responsible for the creation of diverse and potential microbial community. Hence, mangroves are also considered as untapped resources of microbial metabolites and products. Several microorganisms including bacteria, fungi, algae, cyanobacteria, lichens are documented from mangrove habitats. These inhabitant microbes are the good source of secondary metabolites, therapeutic enzymes, food protective agents and mineral solubilisers. However, information on these aspects is very meager and thus the present review is an attempt to compile informations on the sources, properties and application of important microbes from mangrove ecosystems.

Abstract

Many airborne fungal spores are important as allergens, human and plant pathogens, in the bio-deterioration of stored materials like paper, leather, textile, paintings, historical monuments and spoilage of food stuffs. Surveys have been conducted to elucidate the magnitude of the problem. Indoor environment also plays an important role in the precipitation of allergic symptoms caused by fungal spores. The present review briefly highlights some of the qualitative investigations carried out on indoor environments like bakery, building, cold storage, cow shed, dormitory, educational institutions, garbage disposal plant, ware house, hospital, library, food grain godown, leather godown, house dust, poultry house, chick hatcheries, plastic industries and textile industries.

Abstract

Soil is the natural growth media for living plants and microbes, and rhizosphere is niche for a group of soil microorganisms inhabiting around the plant root zone. The warmed and humid agro-climatic conditions of the area aggravate A. flavus contamination and aflatoxin production in maize grains from field to storage. In this article aflatoxin contamination in maize, antagonistic activity of rhizosphere microbes against toxigenic A. flavus, through fungi, antagonistic activity of rhizosphere bacteria against toxigenic A. flavus, bacterization of stored maize seeds for aflatoxin control and inhibition of aflatoxin production in stored maize seed with rhizobacteria has been discussed in detail.

Abstract

Different algal taxa involved in the formation of lichen/s are presented. To-date 43 algal genera, comprising members of Cyanophyta, Chlorophyta and one genus each of families Phaeophyceae (brown algae) and Xanthophyceae (yellow-green algae) are recorded, based on personal observations coupled with literature. The various algal taxa forming different lichens belonging to various families is presented. The taxa Trebouxia de Puymaly and Trentepohlia Born. amongst green algae; Scytonema Ag. and Nostoc Vauch. Among blue-green algae are dominant genera involved in the formation of lichens. The yellow and brown algae are only of rare occurrence.

Abstract

In nature the roots of most of the plants are infected by fungi to form mycorrhizal associations, which play a central role in nutrient capture from the soil. These fungi are found in almost every terrestrial ecosystem; even in pioneer zones or very saline environments, and are reported to represent the second largest biomass component of many terrestrial ecosystems. Mycorrhizal associations are found in a broad range of habitats ranging from aquatic to deserts and alpine altitudes. In natural ecosystems mixed population of mycorrhizal fungi co-exist, with certain fungi becoming dominant in a particular patch and subsequently being replaced as environmental conditions change. Once this equilibrium is disturbed, the population dynamics is disrupted and a biomass may develop towards a few or even for one dominant fungus. Different species of mycorrhizae respond differently in response to the environmental conditions. In the present paper, all major types of mycorrhizal associations in different ecosystems are discussed.

Abstract

Various cropping systems and ecologies illustrate the positive use of chemicals both as fertilizers and pesticides and organic/biological sources within the frame work of Integrated Plant Nutrition and Plant Protection System (IPPS). While considering the organic/biological sources, green manures seem to be the best alternative that may largely suffice to the organic matter requirements of the soil under crop cultivation. Green manuring can be defined as a practice of ploughing or turning into the soil undecomposed green plant tissues for the purpose of improving physical structure as well as fertility of the soil. “Green manure” refers to fresh plant matter, which is added to the soil largely for supplying the nutrients contained in its biomass. The agricultural practices consisting of growing and ploughing under a green crop or addition of a green organic matter in the form of leaves and other succulent parts from outside to improve soil productivity is known as green manuring. In this review, advantages of green manuring, decomposition of green manure,   process of decomposition, factors affecting decomposition and effects of green manuring have been discussed in detail.

Abstract

Production technology has been worked out for crops and several inputs have been recommended which are to be applied in proper quantity at most suitable time to get the maximum return. Nutrition in the form of fertilizers is a must. However, injudicious use of agro-chemicals has resulted in diseases called ‘iatrogenic plant diseases’. Under the circumstances it is important to know the relationship between soil nutrients and plant pathogens which are causing the diseases of economic importance. Author has given adequate emphasis to micronutrients as at a very minor quantity they reduce the disease to an acceptable level. He has also discussed in detail about all the major and minor nutrients required by the plants and how their deficiency or over doses affect the plant pathogens and in turn the diseases.

Keywords:  Soil nutrients, plant pathogens, diseases, relationship

Abstract
 
Much attention has been paid to mycorrhza as a tool for improving the growth and health of plants.  The use of mycorrhiza in agricultureal, horticultural and forestry has been described. The significance of AM fungi in sustainable agriculture has been a subject of growing interest for the past several decades. In sustainable agriculture it is imperative to maximize benefits with low input costs. The fact remains that stable and lower human population is an integral component of sustainable agriculture and more so in an Indian context. Therefore, it is imperative to collect further information on the different aspects of AM fungal symbiosis as discussed in this article so as to utilize this plant microbe symbiotic system for the increased production and productivity in a sustainable manner. This may become possible when the integrated approach is made to study AM fungi right from the isolation of AM fungal spores to the high quality inoculum production and its application in the field. It is also imperative to stimulate new mutualism between mycorrhizal scientists and ecologists. It this regard, recent advancement made in the molecular techniques should be encouraged in order to obtain more authentic results. Adequate field testing of mycorrhizal inoculation and commercial exploitation of the potential benefits of mycorrhiza still rests on the development of suitable technology for mycorrhizal inoculum preparation.

Abstract

Higher plants can be exploited for the discovery of new bioactive products that could serve as lead compounds in pesticide development because of their novel modes-of-action. Different secondary metabolites of higher plants have been reported to exhibit efficacy as fungitoxicants as well as mycotoxin inhibitors. Pest controls using the botanicals are safer to the user and the environment because they break down into harmless compounds within hours or days in the presence of sunlight. The article presents different plant products found efficacious as antimicrobial and inhibitory to mycotoxin secretions by fungi. Their prospect as safe alternatives to synthetic antimicrobials in plant protection has been discussed.

Abstract

Eight species including 1 variety, 2 macro and 6 micro-lichens from Garhwal Himalaya, c 1500-2700 m, have been found to glow and emit fluorescent light, when exposed to long wave-length (254 nm) ultra-violet radiataions. Taxonomy and chemistry of all these species have been critically evaluated. The recorded taxa belong to families Physciaceae (4 species); Pertusariaceae, Rhizocarpaceae (1 species each) and Lichen imperfecti (2 species). The reported taxa are: Buellia hemispherica Singh and Awasthi, B. sub-glaziouana Singh and Awasthi; Pertusaria pertusa (Weig.) Tuck., Pyxine berteriana (Fee) Imsh. var. himalayensis Awasthi; P. cocoes (Swartz) Nyl., Rhizocarpon geographicum (L.) DC.; Lepraria taxonomic species I and II.

Abstract

C. albicans is a diploid, dimorphic yeast producing three morphologic forms: yeast cells, pseudohyphae and true hyphae. It is an opportunistic pathogen causing various types of candidiasis which are on the increase around the globe. It is a polymorphic yeast with its genome organized in eight diploid chromosome with its genome size ranging between 14.3 and 16 Mb having 5,733 to 6, 318 genes. It has two mating-type like (MTL) alleles that control the mating behavior. In its genetic makeup it resembles with Saccharomyces cerevisiae hence classified in the Saccharomycetales. The chromosome number from tetraploid to diploid is brought about by the parasexual mechanism. Keeping in view the increase of incidence of candidiasis due to C. albicans around the globe, it is important to identify the causative organisms to the species level correctly. Morphological, biochemical, germ tube test and growth on different media are often used for identifying isolated yeasts which sometimes takes days to week to develop in culture hence, rapid identification of Candida species in clinical laboratory is becoming increasingly important. Molecular techniques utilizing amplification of target DNA or PCR amplification of conserved regions of the genome and sequencing of the resulting PCR product and restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA, provides quick and precise methods for diagnostics or, identification of Candida species.

Abstract

Agroforestry is an intensive land use management system that integrates trees, shrubs, crops, etc. on a landscape level to achieve optimum benefits. Agroforestry is not only concerned with beneficial effects of one component on another, but it also involves the manipulations of negative effects to minimize their influence on the productivity of the overall system. In an agroforestry, trees and crops compete inevitably for light, nutrients, moisture and other resources. The interactions between tree and crop roots can also have an effect on soil organisms, which play an essential role in the functioning and productivity of agroforesty systems. Arbuscular mycorrhizal fungi (AMF) are one of the most important components of the soil biota in monoculture and agroforestry systems. AM fungi can rehabilitate degraded lands subjected to agroforestry systems and common mycorrhizal network may further enhance the benefits of agroforestry through vertical niche expansion of AMF. However, management practices of agroforestry affect mycorrhization, AMF community composition and diversity of AM fungi. This review focuses on AMF diversity, which plays a significant role in agroforestry systems.

Abstract

A field study of seven arid districts of Rajasthan was undertaken to evaluate the occurrence of selected tree species and arbuscular mycorrhizal fungal (AMF) associations with them. Five genera were identified in the rhizosphere of these selected tree species. A high diversity of AMF was observed which varied between different host tree species. Among the five genera, Glomus occurred most frequently, with four species, Acaulospora and Gigaspora were found with three species, while Entrophospora and Scutellospora was detected with two species. Gigaspora margarita, Gigaspora rosea, Glomus deserticola, Glomus constrictum, Glomus fasciculatum, Glomus mosseae and Scutellospora calospora were the most dominant species. The AMF spore density was not clearly affected by the host tree suggesting that biotic factors may be relatively less important than abiotic/edaphic factors for establishing population pattern. The spore density of AMF had a strong positive correlation with soil pH and organic carbon content and a negative correlation with Olsen’s P content of the soil. The association with AMF of these tree species native to the harsh environmental conditions of the Indian Thar Desert may play a significant role in the re-establishment and conservation of these multipurpose desert tree species.

Abstract

The ability of life to dwell in extreme environments on our mother planet earth right from a thirsty desert environment to highly water saturated oceans, gave the scientists a new area to work in during the 1990’s. The discovery of microbial life which had the ability to survive in conditions which were highly inhospitable for the normal life gave rise to a revolution in the field of science and technology. Extremophiles are one of the most primitive forms of life on earth since they have accustomed themselves to the present day harsh conditions compared to the normal environment. The last few years have seen a massive increase in their discovery, since the areas which once were thought barren by the scientists are now found inhabited by the microbial life. The extremophiles endure conditions right from extreme temperatures, pressure, salt concentration to radiations, metal tolerance, pH etc. During the past few years the industrial applications associated with the extremophiles and their products have led to their intensive exploration. Keeping in view the present and the future scenario, extensive efforts are still required in order to exploit the extreme environments for extremophiles and their products.

Abstract

Today more than 70% of energy is generated by coal-based thermal power plants. Indian coal contains about 40% ash, these power plants generate enormous amounts of fly ash, which is dumped in the nearby areas. This creates not only economy constraints but also imposes serious environmental problems, which are the potential hazards to the ecosystem. However, recent attempts in India and abroad are being made for biological association of fly ash e.g. in soil improvement, in wasteland management and also as a source of essential plant nutrients for nutrient deficient soil in terrestrial eco-system and agro-ecosystem. Fly ash has been found to have manifold advantages in agriculture especially in modification of soil texture and bulk density, in optimization of soil pH, in improvement of water holding capacity of soil, in the improvement of yield as a micronutrient supplement to soil and in creation of conducive condition for better plant growth. In this article utilization of fly ash along with VAM fungi and other microbes especially nitrogen fixers and phosphate solubilizers in removing the constraints of salt affected soils has been discussed.

Abstract

Extensive occurrence of alkaline soils has been reported from the Indo-Gangetic plains of Northern India, which is commonly known as ‘usar’ or ‘reh’. High alkalinity and high exchangeable sodium percentage (ESP) of such soil render it inhospitable for normal crop production. Research efforts over the past few decades have resulted in evolving a number of practices for the reclamation and management of such soils. However, application of biological inputs in combination with some moderately salt tolerant plant species seems to be a better option. AM fungi is well known to enhance the ability of plants to cope with environmental stresses generally prevalent in the degraded ecosystems and stress conditions by providing a number of nutritional and non-nutritional benefits. Since isolates of AM fungi differ in their tolerance to adverse physical and chemical conditions in soil and are better adapted to their native stressed microhabitat, selection of appropriate native AMF isolate is necessary for any large scale reclamation program. Taxonomic diversity of AM fungi in the alkaline soils of Upper Gangetic plains of district Allahabad and adjoining areas has been investigated and it was found that such soils have a detrimental effect on the AM spore population, distribution and diversity. Maximum spore population occurred in winter and minimum in summer. Glomus followed by Acaulospora was the most dominant genus. Acaulospora scrobiculata and Glomus fasciculatum were the most frequent species followed by Glomus mosseae and Glomus aggregatum. Glomus fasciculatum was the most abundant species followed by Glomus mosseae and Acaulospora longula.

Abstract

Microorganisms that harbour in the root region are the most befitting candidates for use as biocontrol agents. The rhizosphere provides the frontline defense for root against attack by pathogens. Pathogens face a complicated phenomena of antagonism from roots. They also compete with each other for site of colonization on the root surface and plant nutrients. The ideal biocontrol agent introduces and /or promotes the antagonists only, whenever required and are most effective in minimizing the wasteful application of inoculum to non-targets. Many genera belonging to bacteria, fungi, actinomycetes and viruses are used as biocontrol agents to combat several important plant diseases. The present review focuses on the use of bacterial antagonists ,particularly rhizobacteria, as biocontrol agents of fungal diseases of plants , their mechanism of action, molecular and genetic basis of antagonistic property and possible genetic manipulations to bring about desirable changes. 

Abstract

The traditional forage production systems and grazing lands are under extreme pressure due to the population growth of both human and livestock, decreasing area and inputs. In the central India, especially the Bundelkhand region, the soil is highly weathered, degraded and have low inherent nutrient, which was further worsened due to use of chemical fertilizers and pesticides, for enhancing crop production to meet the escalating demand of the population.

Abstract

Photosynthetic blooms of toxin producing marine algae are often referred to as “red tides”. Proliferations of freshwater toxin-producing cyanobacteria are simply called “cyanobacterial blooms” or “toxic algal blooms.” Biotoxins of cyanobacteria are water-soluble and heat stable and they are released upon aging or lysis of the cells. The primary types of cyanobacterial biotoxins involves three types of toxins: hepatotoxins (microcystin; cylindrospermopsin and nodularin), which are taken up by the liver and cause weakness and anorexia (loss of appetite); neurotoxins (usually anatoxin and saxitoxin), which effect the nervous system and dermatotoxins (aplysiatoxin and lyngbyatoxin, LPS), which cause skin and mucous irritations upon contact. Unlike freshwater toxic algal blooms, marine algal toxins become a problem primarily because they may concentrate in shellfish and fish that are subsequently eaten by humans, causing syndromes known as paralytic shellfish poisoning/PSP (paralysis due to saxitoxin and gonyaulatoxins), diarrhetic shellfish poisoning/DSP (diarrhea due to okadaic acid) , amnesic shellfish poisoning /ASP(memory disturbances due to domoic acid), neurotoxic shellfish poisoning/NSP (paresthesia due to brevetoxin; also bronchial spasms). Coral reef fishes are the vectors of another algal poisoning, the ciguatera fish poisoning/CFP (paresthesia, warm-cold sense inversion etc., due to ciguatoxins and maitotoxin).

Abstract

The word “mycorrhiza” literally means “fungus root” to describe the mutualistic association between roots of higher plants and certain fungi. Arbuscular mycorrhizae (AM) are the most common and widely occurring of all the mycorrhizal associations and have great economic significance. They belong to the phylum Glomeromycota. Mycorrhizal associations are potential factors in determining the diversity in ecosystems. They can probably modify the structure and functioning of a plant community in a complex and unpredictable way. Currently there is considerable resistance against the use of chemical pesticides and fertilizers, because of their hazardous influence on the environment, and on soil, plant, animal, and human health. Hence, use of biofertilizers and biocontrol agents are recommended in practical agriculture/horticulture/forestry.

Abstract

Ectomycorrhizal symbionts are important natural resources to revive degraded and deforested areas. Artificial inoculum of these mycobionts improves the growth of tree seedlings in nutrient poor soils due to the formation of extensive network of fungal mycelium responsible for the transfer of water and nutrients to the plants. Seedlings of tree species like, Pinus insularis, Quercus griffithii and Schima wallichii with ectomycorrhizae formed by Suillus bovinus, Boletus edulis and Scleroderma citrinum were studied in field condition. The study was conducted at two different stages of degraded sites. Results of the study indicated that seedlings with ectomycorrhizae were able to generate numerous lateral roots of greater length and thereby utilize water and nutrients more effectively than non- mycorrhizal ones The degree of mycorrhizal development varied in different host species with different fungal species. There was a strong relation between the colonization by mycorrhiza and the survival of seedlings. The study has suggested that mycorrhiza has a potential in reclamation of soil and reforestation of degraded land. S. bovinus., B. edulis and S. citrinum successfully colonized and promoted the growth of P. insulates Q. griffithii and S. wallichii respectively in reforestation programmes of degraded lands of Nagaland.

Fruits and vegetables are very important food commodities. India has become the largest producer of fruits and the second largest producer of vegetables. Though the production of fruits and vegetables make us believe in our strength for self-sufficiency, a significant qualitative and quantitative loss occur in the produce from harvest till consumption.  For self sufficiency and also for processing, export and to meet additional requirements, a lot of emphasis need to be given to reduce post harvest losses, besides increasing  production and productivity of horticultural produces. Processing plays an important role in  conservation and effective utilisation of these perishable commodities. However, only less than 2% of total production of fruits and vegetables are processed.  The new industrial policy has placed this sector in the list of high priority areas.

Structure

Structural unit of edible portion of most fruits and vegetables is the parenchyma cells and makes up bulk of cells. A parenchyma cell is rather thin walled and may be polygonal or cubical in shape.  Walls of parenchyma cells in young plants are composed almost entirely of fibrils of cellulose. In parenchyma cells of plants actively metabolising protoplast portion represents only about 5% of total cell volume. This protoplast is rather film like and is pressed against cell wall by large water filled central vacuole.  Protoplast has inner and outer semi permeable membrane layers between which is confined cytoplasm and its nucleus. 

Maturity at harvest is the most important determinant of storage life and final fruit quality. Maturity indices are determined for many fruit, vegetable and floral crops. Harvesting crops at proper maturity allows handlers to begin their work with the best possible quality produce. Produce harvested too early may lack flavour and may not ripen properly, while produce harvested too late may be fibrous or over ripe.
 
Almost all fruits reach their best eating quality when allowed to ripen on the tree or plant (exceptions e.g., pears, avocados, and bananas). In general, fruits become sweeter, more colourful, and softer as they mature. A few fruits are usually picked mature but unripe so that they withstand post harvest handling system when shipped long distances.  Most of  currently used maturity evaluation is based on a compromise between those methods that would ensure the best eating quality and those that provide flexibility in transportation and marketing.  Optimum harvesting stage depends upon type of fruit or vegetable and their final use. Such a division is usually based on their classification according to physiology, environmental conditions, ripening bahaviour and nutritional aspect. Some of the classifications, important for their harvesting and storage are briefed in  subsequent sections.

Various systems were developed to take produce from field to consumer and selection of a particular method depends on several factors, including perishability and value of crop.  One method is to harvest the crop and take it to a packhouse for various unit operations like grading, cleaning, post-grading treatments, and packing for transport and marketing. When deciding upon where to locate a packinghouse, access to field and market point, adequate space for vehicles to enter and leave  packinghouse and ease of access to labor will all be considered.
 
In the simplest packinghouse, produce is delivered in picking containers, immediately after harvest, directly to packers.  Packers then sort, grade, size and pack the produce directly into appropriate transport containers. In this case, each worker must be aware of produce defects, grade and size requirements, and packing methods.

Storage is one of the most important aspects of post harvest handling of fruits and vegetables. The main objective of storage is to extend storage life of fruits and vegetables and increase their period of availability. A substantial quantity of fruits go waste in India due to lack of proper storage facilities. Storage of fruits and vegetables is practiced for various reasons. Proper post harvest handling of fresh horticultural produce helps in extending  period of availability of wide range of  fruits and vegetables round the year, prevention of seasonal market gluts, orderly marketing, better return to grower and  preservation of  nutritional qualities. The   long  term  storage  of  fruits  and  vegetables is expensive and requires high level of technical  knowledge of crop. However, an orderly marketing reduces storage period considerably. 

Processing of fruits  and vegetables into various products which could be preserved for a long time and add to value of product using proper cost effective technology will be a viable tool for improving   economic status of  farmer as well as our country. Modern methods of fruit and vegetable preservation are  broadly classified as follows:

Drying is the oldest known method of preserving food. Drying or dehydration is removal of majority of water contained in fruit or vegetable. Although preservation is the primary reason for dehydration, it also lowers cost of packaging, storing and transportation by reducing both weight and volume of final product. In addition, dried foods add variety to our diets. In the process of drying, sufficient moisture is removed to protect product  from  spoilage. Processing should be done in such a way that food value, natural flavour and characteristic cooking quality of fresh material are retained after drying.

The technique of preserving fruit by the use of low temperature dates from the eighteenth century with the first use of ice house in 1750. Later in 1800s commercial cold storages have come up for the storage of perishables like meat, dairy and fruit products.  The main change took place in the cold store in recent years has been the introduction of multi temperature chambers with humidity control for specific products. Cold preservation can be either refrigeration or freezing. Household refrigerators usually run at 4-7^ºC. Chilling or cold storage usually uses a slightly lower temperature on the basis of the food to be refrigerated. In freezing a temperature of -18^ºC or below is used. Chilling will preserve perishable foods for days or weeks and freezing will preserve foods for months or even years.

Fruit beverages

Fruit beverages are highly refreshing, thirst quenching, appetizing, easily digestible and nutritionally far superior to synthetic drinks. The fruit beverages includes natural and sweetened juices, squash, syrup, cordial, nectar, Ready to Serve (RTS) beverages, fruit juice concentrate and citrus juice barley water. Fruits most commonly used to prepare beverages are oranges, lime, pineapple, grapes, cashew fruit, mango, jack fruit, apple etc. To prepare fruit beverages select fresh, fully ripe, tart, juicy and seasonal fruits.

Fermentation was known to mankind from time immemorial. But, development of biochemical principles of  fermentation  was originated by  Lavoisier in France in 1789 by way of analysing chemical composition of  sugar and its fermentation products such as ethanol, carbon dioxide and  acetic acid. Many products are produced by fermentation process. In fermentation, controlled action of selected microorganisms is used to alter  texture of foods, preserve foods by production of acids or alcohol or to produce fine flavours and aromas which increase value of raw materials.

Minimally processed fruits and vegetables are products that have the attributes of convenience and fresh like quality. Their forms vary widely, depending on nature of unprocessed commodity and how it is normally consumed. In many cases, minimally processed product is raw and cells of tissues are alive, but these characteristics are  not regarded as essential. It is important that products have fresh-like character, regardless of   state of cells.  Minimally processed products for both retail and food service applications have increasingly appeared in market place recently.  In coming years, it is commonly perceived that this industry will have unprecedented growth.

Packaging is an integral part of fruit and vegetable processing. It has  major influence on storage life and on marketability of fresh as well as processed products. Packaging fulfills several functions including containment, facilitating transportation, protection of fruit/vegetable from further damage, protection of environment from contents of package (for example, if the contents are dirty), marketing, product advertising, and stock control.

Transportation and distribution of fruits and vegetables are important stages   of post-harvest loss. In India,  transportation of perishable commodities is in the most precarious stage. For local market, the produce is brought either by bullock cart or tractor trollies. The long distance transportation is mainly by rails and trucks, which is very costly. The basic reason for preference to road transportation is that it takes short transit period.

Quality is an increasingly important factor in the production and marketing of biological products. Consumers are becoming more discerning as their affluence increases, and would be suppliers of products must meet these demands if they are to maintain or increase market share. The term ‘quality’ is one of the most defined terms in use in food industry today. Quality may be defined as ‘The totality of features and characteristics of a product that bear on its ability to satisfy a given need’. The first part, ‘The totality of features and characteristics of a product…..’ concerns objective factors related to the product.  The second part, ‘…… to satisfy a given need’, concerns subjective factors related to user or consumer of goods.

Practical have a significant role in the study of any theoretical subject. Whatever a student learns in theory, he verifies it by actual doing with his own hands.  Thus, these reduce any doubts and misbelieve from his mind. These verifications by actually doing the things are performed in the laboratories.  A laboratory is a place with the basic and essential equipments and adequate facilities for students and teachers to perform and observe experiments.  Plants will continue to provide novel products as well as chemical models for new drugs in the coming centuries.  The arrival of chemical analysis and the characterization of molecular structures have helped in precisely identifying these plants and correlating them with their activity under controlled experimentation.