eChapters

With the sharp development of urban and industrial growth, the area under agriculture is declining year by year. On the other hand, human and animal population is increasing at a very fast rate. So, to meet the growing demand of food and fodders, a vertical increase in yield is required. Crop productivity per unit area can be enhanced with the manipulations of some agronomist practices such as sowing the crops at appropriate time, selection of suitable field for raising a crop, adoption of best planting techniques, use of appropriate seed rates, adoption of proper irrigation schedule, proper and need based application of fertilizers, adoption of zero till technology, green manuring, mulching, addition of well decomposed farm yard manure, timely and proper weed management, selection of recommended crops and crop varieties, proper control of insects and pests, adoption of marketing tips etc.

Tomato

Tomato, a warm season vegetable, requires a long season to produce a profitable crop. The optimum range of temperature for its cultivation should be 21–24ºC. The mean temperatures below 16ºC and above 27ºC are not desirable. The minimum, optimum and maximum soil temperatures for seed germination are 10º, 30º and 35ºC, respectively. Maximum seed germination occurs at 24ºC. Maximum fruit setting occurs at night temperature of 15-20ºC. Flowers fail to set fruit below 13ºC and beyond 38ºC. Formation of red colour due to lycopene is inhibited when temperature exceeds 32ºC. Lycopene is highest at 21-24ºC, while its production drops off rapidly above 27ºC. Tomato plants are highly susceptible to high rainfall areas if proper drainage is not available.

It is grown in many types of soil from sandy to heavy clay. A well-drained fairly fertile loam soil with a fair moisture-holding capacity is ideal for growing its good crop. It prefers a soil having a pH of 6.0 - 7.0. Application of liming is beneficial if it is cultivated in acid soils.

It can be grown almost round the year. The planting seasons vary from place to place. In northern India, for spring summer crop, it is sown in November and transplanted in second fortnight of January. In area, where frost does occur, it is sown in July-August and transplanted in August-September. In hilly areas, it is sown in March-April and transplanted in April-May.

• Harmony is the principle of landscaping.

• Texture is an important element of landscaping.

• Blue is a primary colour.

• Green in a secondary colour.

• Garden furniture is a tangible item.

Indication

Necrosis, Gangrene, Extensive injury of nerve of hind limb.

Malignant neoplasm

Frost bite. Burns

Incurable peripheral vascular disease.

Hip joint is a ball and socket joint which involves cotyloid cavity of os-coxae and head of femur. Hip dislocation can be corrected by external immobilization of the area with POP cast and keeping the animal in a mobile sling for several weeks.

The dislocation involves cotyloid cavity and head of femur and the affected limb deviate outward. (Fig.1)

The fracture site is immobilized extending joint below and joint above level initially with plaster of Paris.(Fig.2)

Cloth bandage is applied over it for support involving joint above and joint below which should not be too tight or too loose.(Fig.3)

Second layer of cloth padding is provided over the first layer to provide more strength and further immobilization.(Fig.4)

Final immobilization is done by application of rubber belt or suspensory bandage. (Fig.5)

At the end the entire animal is kept in mobile sling for proper immobilization for some weeks and housed in limited space(Fig.6)

Histiocytoma is more common in younger dogs below two years of age. It is also observed in all age groups.

Gross examination

Grossly, histiocytoma appears as light pink, smooth to firm, raised round (Figure 77) masses. Alopecia and ulceration are also observed.

McManus’ Method for Glycogen (PAS)
Tissues fix in 10% neutral buffered formalin and process for routine paraffin sections
Protocol
1) Deparaffinise the paraffin sections with two changes (10 minutes each) of xylene.
2) Descending grades of ethanol from absolute to 50% solution (2 minutes each).
3) Hydrate the sections with distilled water 5 minutes.
4) 0.5% Periodic acid solution for 5-6 minutes.
5) Rinsing in distilled water.
6) Schiff’s’s reagent or Coleman’s Feulgen solution for 10-15 minutes.
7) Washing in running tap water for minimum 10 minutes until pink colour develops.
8) Counter stain with Harris’ hematoxylin for 2-3 minutes to stain nuclei.
9) Washing in running water for 2-3 minutes.
10) Quick dip in ammonia water to develop blue colour of the nuclei.
11) Washing in running tap water for 5 minutes.
12) Dehydrate in ascending grades of 90% to absolute ethanol (one minute each).
13) Clearing in xylene, two changes of 30 minutes each.

1. Introduction

Human activities significantly lead to deterioration of ecological systems, affect individual organisms, and disrupt natural ecosystems. The rise in foreign compounds, such as heavy metals and pesticides, in aquatic ecosystems due to human activities has spurred extensive global research efforts (Wester et al., 2002). The Union’s Water Framework Directive (WFD) mandates monitoring programs to assess water body health. In environmental studies, understanding the impact of contaminants on aquatic life requires a multifaceted approach (Ullah and Zorriehzahra, 2015). Biomarkers that observe biological responses at various levels offer crucial insights into the presence and bioavailability of toxicants, complementing traditional methods (Yancheva et al., 2016). These indicators, termed early warning signals, are vital for evaluating exposure, effect mechanisms, susceptibility to contaminants, and enhancing predictive models (Yancheva et al., 2016). Integrating biomarkers into ecological studies provides a holistic understanding of environmental stressors and their implications in aquatic organisms.

2. Why is fish used as a model organism in ecotoxicological studies?

According to the Water Framework Directive (WFD), fish serves as a crucial indicator of river ecological status (Ullah and Zorriehzahra 2015). Their diverse sizes, ages, trophic levels, and sensitivity to various toxicants make them excellent indicators of water contamination (Wester et al., 2002). Fish adapt their metabolic functions in response to environmental changes, which makes them valuable in toxicological research. Monitoring sentinel fish species is a widely accepted method for assessing toxicant accumulation and its impact on human health (Wester et al., 2002). Fish, with their developed osmoregulatory, endocrine, nervous, and immune systems, are preferred to invertebrates in toxicological studies. Fish can absorb toxicants through both waterborne and dietary exposure, allowing the evaluation of contaminant transfer through the food chain. Key tissues for ecological, toxicological, and pathological studies in teleost fish include the gills, liver, and kidney because of their high metabolic activity and tendency to accumulate toxicants (Wester et al., 2002).

A protein that is deposited in various tissues of body called amyloid. Amyloidosis is a disorder of protein folding, in which normally soluble proteins accumulate in the tissues as abnormal insoluble fibrils (or filaments), thus disrupting their function The deposited proteins, generically known as amyloid, damage the structure and function of the tissues and so cause serious disease which is often fatal when it affects major organs. There are several techniques to observe the amyloid which is as follows.
Highman’s Congo Red Technique
Tissues fix in 10% neutral buffered formalin and process for routine paraffin sections The formal saline is a better choice
Protocol
1) Sections to water, removing pigment where necessary.
2) Stain in Congo red solution, 5 minutes.
3) Differentiate with the alcoholic potassium hydroxide solution, 3-10 seconds.
4) Wash in water, stain nuclei in alum hematoxylin and differentiate blue.
5) Dehydrate, clear, and mount.
Observations
Red : Amyloid, elastic fibers, eosinophil granules: Blue : Nuclei
Chemical reagents
Immerse in alkaline sodium chloride solution for 20 minutes.

Pituitary
Herlant (1960) Pituitary Stain I
Tissues fix in 10% neutral buffered formalin or Zenker formol and process for routine paraffin sections
Protocol
1) Deparaffinise the paraffin sections with two changes (10 minutes each) of xylene.
2) Descending grades of ethanol from absolute to 50% solution (2 minutes each).
3) Hydrate the sections with water 5 minutes (remove HgCl2 if present).
4) Stain in erythrosine for 5 minutes.
5) Rinse briefly in distilled water.
6) Stain in Mallory II for 5-10 minutes.
7) Rinse briefly in distilled water.
8) Stain in acid Alizarine blue for 5-10 minutes.
9) Rinse briefly in distilled water.
10) Treat with phosphormolybdic acid for 5-10 minutes.
11) Rinse briefly in distilled water.
12) Differentiate in 70% ethanol

Histomoniasis is a protozoan disease.

Etiology

Histomonas meleagridis.

Hosts

Mainly disease of turkey but has also been reported in chickens, peafowl, grouse, quail and gallinaceous birds.

Mammary tumours arise from the ducts are called as ductal or ductular carcinoma. Some include other tissues such as the myoepithelium and connective tissue between the glands. These have a number of different names including carcinosarcoma, carcinoma or sarcoma in benign tumour, complex adenocarcinoma and malignant mixed tumour. Tumours are also classified by the type of epithelial growth because this influences the probability of recurrence. Tumours that only have myoepithelium or connective tissue are called sarcomas.

• General considerations

 • Scope

 • Microscopic examination techniques

 • Smear

 • Vital staining

• Routine histopathological techniques of sectioning

 • Collection of tissues

 • Fixation

 • Washing

 • Dehydration

 • Clearing

 • Impregnation

 • Casting of blocks

 • Trimming

 • Section cutting

Histopathology is a critical diagnostic method used to examine tissue structure and detect pathological changes caused by infectious agents in fish. In India’s rapidly growing aquaculture sector—encompassing economically significant species such as rohu, catla, tilapia, pangasius, and ornamental fish— histopathology plays a pivotal role in disease surveillance, diagnosis, and research.
As fish farming intensifies across major aquaculture states like Andhra Pradesh, West Bengal, Tamil Nadu, and Kerala, advanced diagnostic techniques like histopathology are becoming increasingly important. These techniques enable early detection of diseases and support efforts in biosecurity and sustainable fish farming.
The process begins with the careful collection of tissues from fish suspected of disease. Key organs examined include gills, liver, kidney, spleen, heart, intestine, and muscle, which are commonly affected in systemic infections of bacterial, viral, parasitic, or fungal origin. Fish samples are collected from ponds, tanks, cages, or RAS units and transported under chilled conditions—preferably on ice—to laboratories such as ICAR-CIFE, state fisheries universities, or certified private centers.
Tissue dissection is conducted under aseptic conditions. Samples are immediately fixed in 10% neutral buffered formalin, the most commonly used fixative in India, to prevent autolysis and maintain cellular architecture. Fixation typically takes 24–48 hours, depending on tissue size and thickness.
After fixation, tissues are processed through graded alcohol dehydration, clearing in xylene (or substitutes), and embedding in paraffin wax. These steps are often performed using automated tissue processors available in regional laboratories. Once embedded, tissues are sectioned at 4–6 microns using a rotary microtome and mounted on clean slides.

  Term anesthesia by Dr Oliver Wendell Holmes (1886).
  Anasthesis (Greece Word) – absence of sensation.

History of Animal Anesthesia
  Paracelsus (1540) produced ether and reported it to have a soporific effect on fowl. 
  Sir Humphrey Davy (1800) suggested that N₂O might have anesthetic property. 
  Chloroform was discovered by Liebig in 1831.
  Dr. Horace Wells (1844) discovered the general anesthetic properties of nitrous oxide. 

Surgery : The branch of medical science which deals with the treatment of injuries, deformities or disease condition by manipulation or operations with the hand. The term surgery is derived from a Greek word – Chirurgia. 
Cheir – Hand 
Ergon – Work 
Veterinary surgery : Veterinary surgery is the surgery which is practiced on animals. 

INTRODUCTION

In biology, fertility is de?ned as capacity to produce off-springs; however, in Soil Science a ?ne difference is made between a fertile and a productive soil. Soil Productivity is the ability of a soil to produce a crop using the entire spectrum of its physical, chemical and biological attributes, while Soil Fertility refers to the capacity of a soil to supply all essential plant nutrients in the desired amounts. Thus, soil fertility management is basically the nutrient management. To put it in simple words, a productive soil is always fertile, while a fertile soil may not be productive for a variety of factors, such as lack of adequate moisture in drier regions or lack of adequate air due to water-logging for most upland crops. Of late, a term ‘ Soil Health’ has been introduced. Soil Health is an assemblage of chemical, physical and biological parameters that closely relate to native or acquired production capacity and sustenance of ecologically important regulatory role (Katyal et al., 2016). It is measured against prede?ned parameters of soil’s status of fertility, compact ability, erodibility and biology. This again tends to relate soil fertility to nutrient content, availability and management.

Introduction

Farming system is complicated inter-related with soil, plants, animals, implements, power, labour, capital and alternative inputs controlled partly by farming families and influenced to varied degrees by political, economic, institutional and social forces that operate at several levels. The farm is viewed during a holistic manner. The farmers are subjected to several socio-economic, biophysical, institutional, body and technological constraints. Farming system conceptually may be a set of components or parts that are reticulate that act amongst themselves. At the centre of the interaction is that the farmer exercise management and selection concerning the sort and results of interaction. It’s the human setting that gives ample condition for development and utilization of a specific system. A farming system clearly is incredibly complicated system. Therefore, any agricultural technology compatible to a specific agroecological state of affairs and socio-economic environment might not be adopted by alternative farmers. Unless each natural and human environment is thought-about, agricultural analysis won’t end in relevant agricultural technology. The income from cropping alone in tiny and marginal farms is hardly ample to sustain the farmers’ family. With the decline in farm size (0.15 ha) because of explosion of population, it’d be more and more tough to provide enough food for the family by the tip of the century. Therefore, the farmer, to be assured of an everyday income for a good living (above the poverty line), a considered mixture of anybody or additional of those enterprises with agronomic crops ought to complement the farm income and facilitate in usage the farm residue/waste. The choice of enterprises should be supported the cardinal principle of minimizing the competition and maximizing the complementarily between the enterprises.