eChapters

Introduction
DNA barcoding is a molecular taxonomic method used to identify species by analyzing variations in conserved DNA sequences that differ among species. The concept of “barcoding” refers to pooling multiple samples and sequencing and analysing them collectively. This technique has been widely applied to identify organisms ranging from simple to complex forms. Unlike other genetic markers, such as short tandem repeats (STR), which are typically used to identify individual organisms, DNA barcoding enables broader species identification. However, identifying species-specific DNA sequences across different taxa can be complex and time-consuming. For accurate species differentiation, the variation in DNA sequences must be sufficiently high to clearly distinguish between closely related species (Antil et al., 2023).
DNA barcode sequences are stored in databases like the Barcode of Life Data Systems (BOLD), which is an online repository containing reference DNA sequences for comparison with unknown samples. These databases store speciesspecific genetic sequences, specimen images, and related data, and also track updates to barcode libraries (Jin et al., 2020). DNA barcoding has progressed alongside genomics research, with large online libraries serving as reference databases for identifying samples from various environments like gardens, forests, and even commercial products (Seethapathy et al., 2019).
As research advances, the number of conserved genomic regions used for DNA barcoding continues to grow. For phylogenetic analysis, the rbcL gene locus is commonly used, along with other regions such as the trnL-F intergenic spacer, matK, ndhF, and atpB (Shaw et al., 2005). The CoxI gene has been wellestablished for barcoding animals and plant species. The trnH, psbA region of the plastid has been proposed as a universal barcode for terrestrial plants (Ward et al., 2005). Several other conserved genomic regions have been established by a “plant working group” for molecular taxonomy (Singh et al., 2012). Despite these advancements, further critical evaluations of the authenticity of these DNA barcoding markers in assessing biological diversity are still needed. Over the last few decades, DNA barcoding has become a widely used tool for confirming species identification, as evidenced by the growing body of research publications on the topic (Rob et al., 2019).Publications on DNA barcoding have seen a notable increase, as shown by a search using specific keywords in the CabDirect database (http://www.cabdirect.org/). This trend highlights the growing importance of DNA barcoding in taxonomic research

Potato is fourth major food crop after rice, wheat and maize in the world.  It produces the highest dry matter, well balanced protein and more calories/unit area and time.  Unlike other food crops, potato tubers contain vitamin-c (17 mg/100 g). It provides carbohydrates, minerals, fibre and a number of vitamin-B groups.  Potato protein is comparable to that of milk and egg.  Unlike European countries, in tropics and sub-tropics, the crop is of short duration and fits well in intensive cropping system.  It can be grown in a wide range of climatic and soil conditions and tubers are available throughout the year. Tubers can be left un-harvested in the soil for some time and are also suitable for long distance transport and storage. Several products like chips, flakes, French-fries, finger chips, granule, disc, cubes and flour are made out of potato tubers.

10.1 Transformation of E. coli by electroporation Principle

Preparation of electro-competent bacteria is considerably easier than preparing cells for transformation by chemical methods. Bacteria are simply grown to mid –log phase, chilled, centrifuged, washed extensively with ice cold buffer containing 10% glycerol. DNA may be introduced immediately into the bacteria by exposing them to a short high voltage electrical discharge. Alternatively, the cell suspension may be snap frozen and stored at-70^oC for 6 months before electroporation, without loss of transforming efficiency.

Since E.coli cells are small, they require very high strengths (12.5- 15KV cm^-1) for electroporation as compared to those used to introduce DNA into eukaryotic cells. Electroporation is temperature dependent and is best carried out at 0-4°C.

The highest efficiency of transformation is obtained when the concentration of input DNA is high (1-10 μg/ml) and when the length and intensity of the electrical pulse are such that only 30-50% of the cells survive in the procedure. Under these conditions, as many as 80% of the surviving cells may be transformed. Higher frequencies of transformation (colonies/ molecules input DNA) are obtained when the DNA concentration is low (?10pg/ml).

The method outlined in this protocol is followed with most strains of E.coli and with plasmids <15kb in size. However, substantial variation in the efficiency of transformation between the strains of E. coli. has been reported. As in case with chemical transformation, linear plasmid DNAs introduced into E.coli by a pulsed electrical discharge transform very efficiently from 10 to 1000 fold but less efficiently than the corresponding closed circular DNA – perhaps because the exposed terminal of linear DNA are susceptible to attack by intracellular nucleases.

Soil is a major source of nutrients needed by plants for growth. The three main nutrients are nitrogen (N), phosphorus (P) and potassium (K). Together they make up the trio known as NPK. Other important nutrients are calcium, magnesium and sulfur. Plants also need small quantities of iron, manganese, zinc, copper, nickel, chlorine, boron and molybdenum, known as trace elements because only traces are needed by the plant. The primary nutrients are nitrogen, phosphorus and potassium, because they are required in larger quantities than other nutrients. These three elements form the basis of the N-P-K label on commercial fertilizer bags. As a result, the management of these nutrients is very important. However, the primary nutrients are no more important than the other essential elements since all essential elements are required for plant growth. Remember that the ‘Law of the Minimum’ tells us that if deficient, any essential nutrient can become the controlling force in crop yield.

The major impact of the two important components of the Karnataka Watershed Development Programme (KWDP) viz farming system intensification and watershed treatment will be on land-based enterprises and land owners, although to a certain extent landless families would also benefit from the increased labour demand through intensification of agriculture. To ensure growth with equity among all the stakeholders in the project, it is imperative to have practical, market-driven, complementary and supplementary income generating activities for weaker sections of the community, including SCs, STs, women, landless persons, families below the poverty line and other vulnerable groups in project districts.  Hence, it is imperative to raise the status of landless families above the poverty line.  The SHGs are to be encouraged to take up viable enterprise and to enhance their fund mobilization by regular savings, investment in viable income generating activities and in time repayment of the loan taken.  The IGAs are meant mostly for the landless and asset less stakeholders in the watersheds.

Insecticides are one of the key control measures to combat the insect pests for sustainable agricultural production in the world. Synthetic insecticides have been only strategy to control the resurgent and resistant insect population in of high value crops not only in India but also in the world. The advent of synthetic insecticides in the mid-20th century made the control of insect and other arthropod pests easy and much more effective, and such chemicals remain essential in modern agriculture despite their environmental issues.

The indigenous pig has been the basis used for pig production for a long period of time. It is small in size. Improved breeds are now being used for grading up the form the basis for pig production in the rural areas. The majority of the pig population in India is of indigenous breeds though population of crossbred and exotic pigs increased by 12.7 percent from year 2003 to 2012. The exotic breed mainly comprises Hampshire, Large White York Shire, Duroc, Landrace, and Tamworth.

Gender analysis is the first and the foremost critical step towards gender responsive planning and programming. It involves collection and analysis of sex disaggregated information. It examines the differences, commonalties and interactions between women and men. Gender analysis examines women’s and men’s specific activities, conditions, needs, access to and control over resources, access to development benefits and decision-making.

Gender analysis helps to clarify the specific and often different capacities, vulnerabilities, needs and coping strategies of men and women, and indicates where opportunities for targeting effective strategies lie. Established patterns of gender inequality and inequity can be explored, exposed and addressed. This may provide opportunities and entry points to develop programs that support efforts to build more equitable gender relations. It aims to achieve positive change for women.

Elements of Gender Analysis

a) Natural: Land, water, forests, rivers etc.

b) Economic: Work opportunities, wages and remuneration, remittance, credit and production inputs.

c) Social: Formal and informal education and social services- religion and cultural norms like dress, defining private and public spaces.

Mushrooms are a nutritious and versatile food commodity that has gained significant importance in India in recent years. With a growing demand for healthy and organic food options, mushroom production has emerged as a lucrative venture for Indian farmers (Fig. 9.1).
• India is home to a diverse range of wild and cultivated mushroom species, with over 200 varieties found across the country. The majority of mushroom production in India is concentrated in the states of Uttar Pradesh, Punjab, Haryana, and Himachal Pradesh.
• Mushroom production in India has witnessed significant growth over the past few decades, driven by advances in technology, improved farming practices, and increasing demand. According to the Indian Council of Agricultural Research, the country produces over 1.5 million metric tons of mushrooms annually, with a value of over `3,000 crores.
 • Mushrooms are an important crop in India due to their high nutritional value, medicinal properties, and economic significance. They are an excellent source of protein, fiber, and various essential vitamins and minerals. Mushrooms are also rich in antioxidants and have been found to have potential health benefits, including reducing inflammation, improving cardiovascular health, and boosting the immune system.

Abstract
The aquaculture industry has undergone significant transformations in recent decades, yet issues like feed waste, pollution, and economic inefficiencies remain unresolved. One possible remedy is precision feeding, which involves providing fish with precise nutrition tailored to their physiology. Precision feeding is an innovative and sustainable approach in aquaculture to optimize feed utilization and reduce waste. Traditional feeding practices often result in overfeeding, nutrient imbalances, and environmental degradation. By matching the feed supply to the dietary requirements of various fish species, precision feeding improves feed efficiency, growth, and health. Advanced technologies, including demand feeders, acoustic systems, sonar imaging, and intelligent feeding devices, enable real-time monitoring and control of feeding processes. These systems reduce environmental pollution while improving operational profitability. Precision feeding has potential, but adoption is hampered by factors like expensive costs, complicated technology, and the requirement for specialist knowledge. As aquaculture grows, precision feeding offers a promising pathway to sustainable practices by integrating automation, artificial intelligence, and real-time monitoring systems. This approach ensures ecological responsibility and aligns with industry goals for productivity and economic viability.
Keywords: Precision feeding, fish nutrition, feed waste, intelligent feeding systems and sustainability

The evolutionary classification based largely on the typological methods has been adopted a number of fish taxonomist. Foremost among them are Gunther and Cope (1871) Boutenger (1904); Jordan (1923); Regan (1929); Berg (1940) and Romer (1959). Out of the various schemes proposed by aforesaid taxonomists, there is no agreement among the ichthyologists regarding the classification of fishes. Fishes constitute more than one half of all vertebrates comprising around 40,000 species.

The classification of Berg (1940) is most widely accepted, it is based upon the conclusions of Jordan (1923) and Regan (1929). Many ichthyologists consider Bergs classification as a minor revision. Berg scrutinizing the merits of the other classification proposals and make advanced classification, which is accepted all over the world.

Abstract

Children today are playing an important role in family buying decisions like never before. They inform their parents regarding the modified use of products. Realizing this, marketers have changed the market into direct market for the children products. The children involved with their products are more likely to pay attention to their advertising, to evaluate their brands carefully and to become brand loyal. Children today are more brand conscious and have more awareness about the brands because the market for children products is rapidly growing. The media has affected the decision making role of the children and now they are more aware of new products because of media messages like advertisements but still final decision for buying a product is in the hands of parents. Advertisements produce brand consciousness among the children and they enjoy switching from one brand to another as it gives a feeling of adventure to them. Both parents and children agree that there is rapid growth in the market for children products over the last few years because marketers have come to know that children are the influencers of today but bread winners of tomorrow and hence, they are a force to reckon with.

e-SAP is one of the important emerging and efficient dissemination tool for agricultural pest management. e-SAP refers to electronic solution for  agricultural pest management. e-SAP is a ground – breaking application built on the backdrop of agricultural pest management. Its foundation lies on a unique platform that facilitates the storage access and transfer information between all stakeholders in agriculture. It enables rapid and delivery of farm data in various forms including multimedia to researchers, policymakers and other users in real time.

Abstract
 

Ensure enough food and water to the growing world population and preserve the biodiversity of our planet are among the major challenges of our immediate future. These challenges are essentially addressed to the agricultural world, which is constantly evolving to satisfy the most basic needs of the humankind. The approaches and the possible answers to these big challenges are many and, even if partial, each sustainable solution or innovation can contribute to improve the quality of our life. To overcome the barriers for food and nutritional security microgreens is one of the possible innovative approach in this 21st century. A microgreen is a young vegetable green that is used both as a visual and flavor component or ingredient primarily in fine dining restaurants. Fine dining chefs use microgreens to enhance the attractiveness and taste of their dishes with their delicate textures and distinctive flavors. Smaller than “baby greens,” and harvested later than sprouts, microgreens can provide a variety of leaf flavors, such as sweet and spicy. They are also known for their various colors and textures. Among upscale markets, they are now considered a specialty genre of greens that are good for garnishing salads, soups, plates, and sandwiches.

Any organization which is in the process of scaling heights should find job motivation for its personnel. The job motivation of personnel in any organization should support the personnel and improve productivity and services. The chapter gives accounts of the significant findings of the study, followed by tenability of hypotheses and further areas of research in the related areas, suggestions and conclusion. The present study has been undertaken to find out the job motivation of LIS professionals working in Kerala's major eight state universities. The data of the study were collected from 266 (two hundred and sixty-six) Library and Information Science professionals. The study's objectives are examined with reference to the analysis of data gathered through questionnaires and realized. 

Today, health promotion of the poor has emerged, worldwide, as a vital area for policy research and action. Healthcare is the responsibility of the constituent states and territories of India.
Food security: Gender dimensions
Health standards have improved gradually over a period of years. But the burden of ill health is borne disproportionately by different population subgroups and people of lower socio-economic status, especially women, as they consistently experience poor health outcomes. Gender inequality leads to food insecurity and is also its leading cause. National food security is largely dependent on agricultural sector. The FAO articulates that food security is achieved when people at the individual, household, national, regional and global levels, at all times, have physical, social and economic access to sufficient, safe and nutritious food to meet their dietary needs and food preferences for a healthy and active life.
Rural women rarely own a control land and lack access to agricultural inputs, technology, training, extension services and marketing services. Despite being major contributors to agricultural production, they have little or no access to the means of production. They face restrictions in mobility due to social conditions, hence face a narrower range of labour markets than men including harsh working conditions and lower wage rates. This is insufficient for them to overcome absolute poverty. Added to this, the role of girl child as an unpaid contributor to the household production is another labour phenomenon of agriculture. In many families, girls and women suffer from severe anaemia, malnutrition and a malady of diseases. Women who are considered and counted in the national statistics as ‘cultivators’ are largely unpaid workers. In many families, females suffer from severe anaemia, malnutrition and a malady of diseases. ‘Feminisation of agriculture is being witnessed as there is higher proportion of female labour in comparison to male labour. They often engage in unpaid family agriculture work and subsistence farming.

Introduction
DNA fingerprinting is a powerful molecular technique used to identify individuals based on their unique genetic makeup. First developed by Sir Alec Jeffreys in 1984, this method has revolutionized fields such as forensic science, paternity testing, genetic research, and biodiversity conservation. We present here the account of variations in DNA sequences, particularly of hypervariable regions like minisatellites and microsatellites in reference to the DNA fingerprinting. Advances in PCR, hybridization-based markers, and next-generation sequencing (NGS) have further refined its accuracy and efficiency enabling precise identification of genetic relationships. Additionally, genetic distance analysis and clustering methods help in understanding evolutionary relationships, population structure, and genetic diversity among species. These methods serve as tools to quantify the magnitude and order of DNA sequence polymorphisms which exist at population level.
DNA Fingerprinting
History of DNA Fingerprinting: It was 10th date of September 1984, when geneticist Sir Alec John Jeffreys (the father of DNA fingerprinting) wrote three words - “33 autorad off” - in his red desk diary. The implicit phrase suggested the completion of an experiment by him pertaining to the study about inherited illnesses descended through families. He was one of the first to discover inherited variation in human DNA that can be utilized to resolve issues of disputed parentage and forensic investigations.

Summary

Stem cells are undifferentiated cells which have the potential to divide and multiply indefinitely and can be differentiated to any type of cell of the body of an organism. Due to its peculiar characteristics stem cells are being experimentally used to cure certain dreaded diseases of the humans including different haemoglobinopathies. The stem cells isolated from bone marrow have been cultured, modified and differentiated to get various blood cells like erythrocytes, leukocytes, and thrombocytes etc. Recently haematopoietic stem cells have been transplanted to the sickle cell and beta thalassaemia patients successfully. Gene therapy using the embryonic and cord blood stem cells have been experimentally tried successfully to cure transgenic sickle as well as thalassaemic mice. Induced Pluripotent stem cells (iPS) generated from autologous skin has also been used successfully for treatment of transgenic sickle cell anaemia mouse.

Introduction

Ration formulation is a process by which different feed ingredients are combined in a proportion necessary to provide the animal with proper amount of nutrients needed at a particular stage of production. It requires the knowledge about nutrients, feedstuffs and animal in the development of nutritionally adequate rations that will be eaten in sufficient amounts to provide the level of production at a reasonable cost. The ration should be palatable and will not cause any serious digestive disturbance or toxic effects to the animal.
 
Different species, strains or classes of animals have different requirements for energy (carbohydrates and fats), proteins, minerals and vitamins in order to maintain its various functions like maintenance, reproduction, egg production, milk production and/or growth.
  
Least-cost ration formulation for non-ruminants is being prepared by  linear computer programming. For ruminants the approach used is to maximize the use of roughage, then, concentrate is used to supply the deficient  nutrients to meet the requirement of the animals. It should be remembered that rations for ruminants are formulated on dry matter basis due to wide variations in moisture content of feeds especially roughage.

I. INTRODUCTION

Horticultural crops include fruits, vegetables, flowers, plantation crops, spices, and medicinal and aromatic plants have emerged as a major economic activity in India. It contributes 30% GDP from 11.73 % of its arable land area. Horticulture crops cover over 23.4 million hectares of area, which is approximately 12.7% of the total gross cropped area of the country and contributes 18-20% of the gross value of India’s agricultural output (NHB, 2013). India is the world’s second largest fruit and vegetable producing country accounting for 77.7 million metric tonnes of fruit and 159.5m M tonnes of vegetables (NHB, 2013). Besides, about 5.66 million tonnes of loose flowers, spices, mushroom and aromatic plants are also produced. The country produces 41% of world mangoes, 23% banana, 24% cashew nut, 36% green peas, 10% onion and 30% cauliflower and accounts for 12% of world fruit and 13.25% of vegetable production (NHB, 2013). Owing to wide diversity of agro climatic conditions, almost all types of temperate (apple, peach, plum, apricot, cherries and nuts), subtropical (mango, guava, papaya, etc), tropical (banana, pineapple etc), arid fruit (ber and aonla), plantation crops (coconut, cashewnut and arecanut) and diverse variety of vegetables are grown in India. The export of fresh fruit and vegetables from India in 2006-07 was estimated at 9.84 Lakh tonnes valued at Rs 2411.70 crores. Mango, grapes, apple, onion, potato, green vegetables like okra, bitter gourd and green chillies were the major items of export. Similarly, India exported 5.97 Lakh tonnes of processed foods comprising of mango pulp, juices, concentrates, dried and processed vegetables, pickle and chutney, alcoholic and non-alcoholic beverages worth Rs 2818.1 crores (APEDA, 2012) (Table 1 to 3).