eChapters

Synonyms : Johne’s disease, Chronic  specific enteritis of cattle, Chronic bacillary dysentery
 
Paratuberculosis (Johne’s disease), an infectious disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), has been recognized as a major disease of ruminants for over a century. It is primarily a disease of domestic and wild ruminants and causes heavy economic losses  in cattle, sheep and goat industry. It is spreading insidiously among animal population worldwide.  Recently it has been shown  that  the disease is not restricted to ruminants only and the host range is expanding to cause disease in other species including Crohn’s disease in humans. In India, the  disease has been occurring  since early 1930s in cattle, sheep and goats in almost every state. The disease in ruminants is clinically characterized by chronic intermittent profuse diarrhea, weight loss, emaciation and finally death.

For the laboratory diagnosis of the parasitic infections, it is essential to examine various excretory-secretory products, viz., faeces, urine and nasal discharges as  examination of such material is essential for clinical diagnosis. It is extremely rare to get an animal completely free from parasites and at the same time all animals may not show signs of disease. Since, most of the important helminths and coccidia inhabit intestinal tract, therefore, clinical diagnosis of gut dwelling helminths and coccidia depends mainly on identification of ova/oocyst in faecal samples.

Examination of faecal sample for helminthic infections

Gross Examination: It precedes the microscopic examination of faecal sample and includes collection of information as below:
    a)    Consistency: Condition of the faeces should be noted as soft, watery (diarrhoeic) or hard but it should be kept in mind that the description varies with the concerned animal species.
    b)    Colour: It is to report the unusual faecal colour, if any as mud coloured foul  smelling faeces in calves indicate ascarid infection; tarry coloured faeces in dog is pinpointing of hookworm infection.

Mange or parasitic dermatitis is caused by the infestation of mange mites e.g. Sarcoptes, Psoroptes, Demodex spp. and a range of other mites. The condition is characterized by itching, rubbing of the infested parts of the body against hard objects. Lesions include hair loss, dry, rough, thickened and wrinkled skin accompanied by the presence of scab and foul odour in some cases. Some mites burrow into the host epidermis (Sarcoptes) while others spend their entire life cycle on the skin surface (Psoroptes). Most mites are microscopic or less than 1mm in length; therefore skin scrapings are taken from the affected area of animals suspected to be suffering from mange or parasitic dermatitis.

Collection of blood

Blood sample should be collected in a capped clean and dry vial containing an appropriate amount of anticoagulant. The commonly used anticoagulants are ethylene diamine tetra acetic acid (EDTA) @ 1-2 mg/ml and heparin @ 5 I.U./ml of blood.

HELMINTHS 
Collection of parasites

Fresh helminth parasites should be collected, as far as possible to achieve better results in staining. This can best be done by conducting a post­mortem examination of the animal for which the procedure given below should be followed. On opening the body and before removal of the viscera, examine the body cavity (for free lying parasites, e.g. Setaria), subcutaneous tissue (for Parafilaria and Hypoderma larvae) and the surfaces of internal organs for spot or nodules that might contain parasites. Remove them for histopathological examination (in 10 % formalin). Remove the visceral organs separately and place them in different sized enamel trays.

Diagnosis of parasitic infections is routinely done with conventional parasitological techniques as described earlier. However, when the parasitaemia is too low to be detected by the conventional methods as in subclinical infections and/or carrier animals advanced diagnostic tools are used for the early, sensitive as well as specific diagnosis.

Serological techniques

Diagnostic techniques used mostly in laboratories need various reagents and equipments to perform the test. However, a diagnostic kit is the extrapolation of the same method by packing it in a user friendly format, accompanied by all or most of the reagents required to perform the test and can also be done outside the laboratory or under field conditions.

Introduction

The tenet “Let food be thy medicine and medicine be thy food,” said by Hippocrates nearly 2,500 years ago, is receiving renewed interest. In particular, there has been an explosion of consumer interest in the health enhancing role of specific foods or physiologically active food components called as functional foods [1]. Clearly, all foods are functional, as they provide taste, aroma, or nutritive value. Within the last decade, however, the term functional as it applies to food has adopted a different meaning - that of providing an additional physiological benefit beyond that of meeting basic nutritional needs.

Introduction

Vast proportions of the population of the world are dependent on plants as their principal source of food either directly or indirectly. Recent decades have also seen increased numbers of people turning to alternative medical therapy based upon traditional folk medicine or food as a form of medicine. These alternative therapies emphasise the importance of a ‘good diet’ in maintaining and restoring health. Under these circumstances, vast majority of population may see diet therapy, dietary supplements and non-invasive alternative therapies as a safer, less technological and more natural way of maintaining health and treating and preventing individuals from illness. These alternatives may be seen as empowering the consumer and shifting the locus of control away from the medical establishment. This chapter covers many crucial topics to understand the chemistry and bioactivity of the active compounds of plant origin. It also aims to cover the latest research on the bioactive compounds.

Introduction
 
Dairy ingredients are of significant importance in the formulation of many food products. Additionally, the incorporation of dairy ingredients, comprehensively recognized by the consumer as “natural” intensifies the odds of success of packaged foods and adds functional properties of the dairy ingredients in the targeted product. Dairy ingredients can be extracted from fluid milk in the form of cream, butter, condensed milk, dry milk, cheese, and whey products [1]. They provide beneficial functionality to foods, such as delivery of key nutrients, water management, fat-holding capacity, emulsification capability, viscosity creation, gel formation, and foam generation apart from providing desirable attributes of texture and flavour. Various ingredients and claims associated with dairy foods are shown in Table 3.1.

Introduction

The term probiotic is derived from Greek and means ‘for life’ as opposed to antibiotics which means ‘against life’. The history of probiotics began with the consumption of fermented foods which was first observed in ancient Greeks and Romans [1]. The term, probiotic, as is used today was first used by Parker in 1974. Parker defined ‘probiotic’ as substances and organisms which contribute to intestinal microbial balance. In 1989, the term was modified further by Fuller. Thus, probiotic is a live microbial supplement which affects the host’s health positively by improving its intestinal microbial balance. This definition was broadened to include mono or mixed culture of live microorganisms which benefits animals or man by improving the properties of the indigenous microflora [2]. Some authors have interpreted probiotics as microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host.

Introduction
 
Whilst food has long been used to improve health, our knowledge of the relationship between food components and health is now being used to improve food. Although most foods can be considered “functional”, in the context of this review, the term is reserved for foods and food components that have been demonstrated to provide specific health benefits beyond basic nutrition. Widespread interest in select foods that might promote health has resulted in the use of the term “functional foods”. Seaweeds are known for their richness in polysaccharides, minerals and certain vitamins [1], but they also contain bioactive substances like polysaccharides, proteins, lipids and polyphenols, with antibacterial, antiviral and antifungal, as well as many such other properties [2]. This gives seaweed great potential as a supplement in functional food or for the extraction of compounds. Physiologically active substances in marine algae are classified into two types based on the difference in the mechanisms viz. (i) non-absorbed high-molecular materials like dietary fibres, and (ii) low molecular materials, which are absorbed and which affect the maintenance of human homeostasis directly. Seaweeds are among the first marine organisms chemically analyzed, with more than 3,600 articles published describing 3,300 secondary metabolites from marine plants and algae, and they still remain an almost endless source of new bioactive compounds [3].

Introduction

Spices and aromatic herbs have been used since antiquity as preservatives, colorants, and flavour enhancers. Spices, which have long been the basis of traditional medicine in many countries, have also been the subject of study, particularly by the chemical, pharmaceutical, and food industries, because of their potential use for improving health. Both in-vitro and in-vivo studies have demonstrated how these substances act as antioxidants, digestive stimulants, and hypolipidemics and show antibacterial, anti-inflammatory, antiviral, and anti-carcenogenic activities. These beneficial physiological effects may also have possible preventative applications in a variety of pathologies. The chapter presents an overview of the potential of spices and aromatic herbs as functional foods ingredients.

Introduction

Encapsulation may be defined as a process to entrap one substance (active agent) within another substance (wall material). The encapsulated substance, except active agent, can be called the core, fill, active, internal or payload phase. The substance that is encapsulating is often called the coating, membrane, shell, capsule, carrier material, external phase, or matrix. In the food industry, encapsulation process can be applied for a variety of reasons. Encapsulation is a useful tool to improve delivery of bioactive molecules (e.g. antioxidants, minerals, vitamins, phytosterols, lutein, fatty acids, lycopene) and living cells  (e.g. probiotics) into foods [1]. In most cases, encapsulation refers to a technology in which the bioactive components are completely enveloped, covered and protected by a physical barrier, without any protrusion of the bioactive components. Also, encapsulation has been defined as a technology of packaging solids, liquids, or gaseous materials in small capsules that release their contents at controlled rates over prolonged periods and under specific conditions. Produced particles usually have diameters of a few nm to a few mm.

Introduction
 
In recent years, there has been a growing interest in the functional foods because they can provide physiological benefits additional to nutritional and energetic, as for instance, antihypertensive, antioxidant or anti-inflammatory [1]. A functional food can be defined as a food that produces a beneficial effect in one or more physiological functions, increases the welfare and/or decreases the risk of suffering a particular disease. Furthermore, new types of products, derived from food, called nutraceuticals have recently been developed. These products, usually employed as food supplements, are marketed as tablets and pills, and can provide important health benefits.

Introduction

Spray drying process has been used for decades to encapsulate food ingredients such as flavors, lipids, and carotenoids. During this drying process, the evaporation of solvent, that is most often water, is rapid and the entrapment of the interest compound occurs quasi-instantaneously. This required property imposes a strict screening of the encapsulating materials to be used in addition to an optimization of the operating conditions. Likewise, if the encapsulated compound is of hydrophobic nature, the stability of the feed emulsion before drying should also be considered.

Introduction

Since 1990, there has been a lot of interest in functional foods and nutraceuticals, particularly because of their impact effects on human health and the prevention of certain diseases. Apart from growing good quality natural products, there are challenges in extracting, refining, analyzing active ingredients, and evaluating safety before an effective, reliable product is made [1,2]. Special attention needs to be focused on separation and purification mainly because 40-80% of the cost of production is assigned to separation and purification unit operations in making functional foods and nutraceuticals. There are also several carotenoids and polyphenol products that have been identified from various plants and a single plant could contain highly complex profiles of these compounds. These are labile to heat, air, and light, and they may exist at low concentrations in the plants. This makes the separation and detection of nutraceuticals a challenging task. The membrane techniques used for the separation of nutraceuticals from the natural products, as well as their applications and influencing factors are discussed in this chapter [3, 4].

Introduction

The emerging functional foods market presents a considerable challenge to all manufacturers to keep abreast of the use of adequate packaging materials for their products. The interest in functional foods is intrinsically linked to the potential health benefits of their bioactive components. Preserving these bioactive components is, therefore, a crucial factor to providing the anticipated health benefits of functional foods. However, recurring failure in maintaining the quality of functional foods has been reported. Functional foods include a wide variety of products such as prebiotics and probiotics, dried foods (fibers, tea, herbs, etc.), fermented products (yogurts, kefirs, vegetables), and fresh fruits and vegetables. Their bioactive components (minerals, vitamins, fibers, peptides, proteins, w–3 polyunsaturated fats, antioxidants, enzymes, and symbiotics) range from very simple molecules to complex living organisms such as bacteria.

Introduction
 
The emerging functional foods market presents a considerable challenge to all manufacturers to keep abreast of the use of adequate packaging materials for their products. The interest in functional foods is intrinsically linked to the potential health benefits of their bioactive components. Preserving these bioactive components is, therefore, a crucial factor to providing the anticipated health benefits of functional foods. However, recurring failure in maintaining the quality of functional foods has been reported. Functional foods include a wide variety of products such as prebiotics and probiotics, dried foods (fibers, probiotics, tea, herbs, etc.), fermented products (yogurts, kefirs, vegetables), and fresh fruits and vegetables.

Introduction
 
Developments in innovative food packaging technologies have evolved in response to the need for protection of the food product containing bioactive component from both external and internal environments and in response to consumer expectations for health claims, convenience and product safety. Consumers around the world are willing to pay extra for guaranteed product freshness delivered by the type of packaging. Many new packaging developments have focused on extending the shelf life of the product and on delivering a higher quality product to the consumer. These developments would not be possible without significant advances in the materials used in packaging and the incorporation of various types of sensors into food packaging. This chapter outlines the innovative packaing solutions in the production of functional food.

Introduction
 
Cardiovascular disease (CVD) is still a major cause of death in Western populations and is becoming an important cause of morbidity and mortality worldwide. Thanks to advanced medical knowledge and treatments, many patients survive an initial event. Because of that, prevention of secondary CVD is a growing task for nutritionists and other health professionals. Cardiovascular risk can be reduced by lifestyle changes, one of which is diet. There is now substantial evidence from epidemiological and clinical studies that a diet rich in fruits, vegetables, unrefined grains, fish and low-fat dairy products, and low in saturated fats and sodium, can reduce the risk of coronary heart disease and hypertension.

Introduction
 
Diabetes shows a wide range of variation in prevalence around the world and it is expected to affect 300 million by the year 2025. In a prevention framework where banning policies and educational strategies lead the interventions, functional foods (FFs) with their specific health effects could, in the future, indicate a new mode of thinking about the relationships between food and health in everyday life. Functional ingredients, such as stevioside, cinnamon, bitter melon, garlic and onion, ginseng, Gymnema sylvestre and fenugreek, have been addressed for their specific actions towards different reactions involved in diabetes development. New strategies involving the use of FF should be validated through large scale population trials, considering validated surrogate end points to evaluate the effect of FF in prevention of chronic diseases such as type 2 diabetes mellitus.

Introduction

The search for weight controlling foods began in the mid 19^th century when industrialization led to widespread obesity. Over the years, people have tried to lose weight by avoiding particular food groups like carbohydrates, eating specific foods such as cabbage soup, or adding a certain ingredient, like vinegar or grapefruit to each meal. Recently scientists have demonstrated that green tea, dairy products, and cinnamon have the potential to improve weight control by altering fat and/or carbohydrate metabolism at the sub-cellular level. Considerable research is needed, however, before these food items can be used to develop effective weight management products.

Introduction
 
The future of functional foods is bright and there are several reasons for the growing interest in “functional” foods, such as awareness of personal health deterioration, led by busy lifestyles with poor choices of convenience foods and insufficient exercise; increased incidence of self-medication; increased level of information from health authorities and media on nutrition and the link between diet and health; scientific developments in nutrition research; and a crowded and competitive food market, characterized by pressurized margins. Now, the consumer is well aware of the relationship between diet and health. Consumers understand that the risk for chronic diseases such as heart disease, stroke, colon cancer, and type II diabetes is possibly preventable by modification in their diet pattern. This scientific progress in understanding the relationship of diet to disease, along with increasing health care costs and the consumer’s desire to initiate healthy eating and lifestyle habits, makes this a potentially fertile ground for the development of food products that meet these demands.

In most countries there is no legislative definition of the term drawing a border line between conventional and functional foods is challenging even for nutrition and food experts. Functional foods do not have a specific place in food law; consequently there is a great deal of confusion about what they are, could or should be. A common perception is that functional foods are foodstuffs either with one or more specific added ingredients (components), or certain substances of common ingredients (components) removed and which are claimed to have a positive influence on human health or well being. The term ‘functional foods’ for this group of products is considered to be an unfortunate one since all foodstuffs are or should be functional and a more appropriate term might be helpful.

WORLD HISTORY

Work on ornamental crops was first initiated in Krelage Nursery founded by Ernst Heinrich Krelage for bulbs, lilium and dahlias in 1811. During 1895, Taiwan Agricultural Research Institute was established for ornamental crops. The first yellow corn Golden Bnatam was listed in Burpee’s Catalogue in 1902. In 1909 Primadonna F1 hybrid variety of begonia was released by the Benary Seed Company in Germany and in the same year Atlee established seed farms at Floradale in California, Erfurt in Germany Anglia in UK and Kashmir valley in India for annual garden flowers. Luther Burbank did excellent breeding works on ornamental trees, flowers and fruits in 1926.

INTRODUCTION

Various procedures which are used for genetic improvement of crop plants are known as breeding methods. The breeding approaches that are more commonly used for genetic improvement of crop plants are called conventional breeding methods. Following conventional breeding methods were followed in improvement of crops.
    (A)    Plant introduction
    (B)    Pureline selection
    (C)    Mass selection
    (D)    Progeny selection
    (E)    Synthetic and composite breeding
    (F)    Pedigree method
    (G)    Bulk method
    (H)    Backcross method
    (I)    Heterosis breeding
    (J)    Clonal selection

MUTATION BREEDING

Mutation refers to sudden heritable change in phenotype of an organism or permanent change in the number, kind and sequence of nucleotides in genetic material, while individuals showing these changes are known as mutants. Mutation was first discovered by Wright in 1791 in male lamb which had short legs. Hugo de vries coined the term mutation. In crop improvement the breeders are always look for wide range of variability and stabilization of desirable traits. Mutation breeding is very useful in inducing new variability, which is an essential requirement of any plant breeding programme. The modern day cultivars are the resultants of various breeding techniques like selection, hybridization, tissue culture and mutagenesis including some biotechnological tools. Novelty in commercial traits like colour, size, shape, length, post harvest life of flowers etc. are always valued and preferred by consumers. Mutation breeding by exposing planting material with irradiation has play vital role in inducing novelty in a number of flower crops.

MOLECULAR BREEDING

Molecular breeding is the application of molecular biology tools, often in plant breeding.

Genetic markers

Genetic marker is defined as a gene whose phenotypic expression is usually easily discerned, used to identify an individual or a cell that carries it, or as a probe to mark a nucleus, chromosomes or locus (King and Stansfield, 1990). Three broad classes of genetic markers are morphological markers, biochemical markers and DNA or molecular markers.
 
(1) Morphological markers

Morphological markers are based on the visually assessable traits viz., morphological and agronomic traits. However, they are limited in number.

INTRODUCTION

Hybrid crops are derived from an established and well-proven breeding method used in ornamental sectors. The development of hybrid crops has been one of the major factors behind a dramatic increase in global crop yields. Hybrid is produced by crossing between two genetically dissimilar parents. Pollen from male parent called pollen parent will pollinate, fertilize and set seeds in female called seed parent to produce F1 hybrid seeds. For production of a hybrid, crossing between two parents is important, the crossing process will results in heterosis or hybrid vigour. Heterosis is defined as the increase in size or vigour of a hybrid over its parent. This boost in performance, combining the best yield, quality and agronomic characters from each parent, is the result of hybrid breeding.

Intellectual Property Rights (IPR) are legal rights, which result from intellectual activity in industrial, scientific, literary and artistic fields. These rights safeguard creators and other producers of intellectual goods and services by granting them certain time-limited rights to control their use. Protected IP rights like other property can be a matter of trade, which can be owned, sold or bought. These are intangible and non exhausted consumption. The term “intellectual property” denotes the specific legal rights which authors, inventors and other IP holders may hold and exercise and not the intellectual work itself (Chadha, 2013). Intellectual property rights are essentially territorial in nature so, where registration is necessary, for patents, registered designs and trademarks, these will not exist in other countries unless registration has been sought and obtained there.

Alstroemeria belongs to the family Alstromeriaceae. Alstroemeria flowers third in terms of popularity and foreign exchange earnings in Kenya after roses and statice. Alstroemeria is named after a Swedish botanist “Baron Klas von Alstroemer”. The popularity of this flower is still growing in the global perspective. It is one of the quickest growing flowers with varying colours like purple, lavender, salmon, red, pink, white, orange, yellow and bicolour. It is grown for cut flowers but is also popular as a potted plant. It is grown in the higher ranges of the Western Ghats, Himachal Pradesh, Jammu & Kashmir and Karnataka in India.

Anthurium (Anthurium andraeanum Linden) belongs to the complex family Araceae, is a slow growing perennial that requires shady, humid conditions as found in tropical forests. The name anthurium is derived from Greek word “anthos” meaning flower and “oura” tail referring to the spadix. Thus, anthurium is also known as “tail flower” (Tajuddin and Prakash 1996). Anthuriums are gaining popularity due to higher returns per unit area and their beautiful and attractive long lasting flowers. Anthurium ranks ninth in the global flower trade and commands a respectable price both for its cut flower and whole plant. Commercial production has focused on two major species viz. A. andraeanum and A. scherzerianum. A. andraeanum is grown mostly for cut flower production, the main production areas being Hawaii, The Netherlands, and some other tropical and subtropical countries. A. scherzerianum is sold as a flowering potted plant, with main production areas located in Europe. In India, the anthurium cut flower industry is still in its infancy. At present anthuriums are mostly grown in some small gardens and nurseries. The important states cultivating anthuriums are Assam, Kerala, Tamilnadu (Salem), Karnataka (Coorg) and Mizoram where the favourable climate exists (Agasimani et al., 2011).

Antirrhinum is a genus commonly known as snapdragon belongs to family Scrophulariaceae. Snapdragon is one of the flowering plant present with vast genetic diversity in India. The term Antirrhinum is derived from the Greek word “anti” means like and “rhin” means a nose, referring to the snout like shape of the flower. The cultivated Antirrhinum majus is grown in tropical, subtropical and temperate countries. The common name snapdragon, originates from the flowers reaction to having their throats squeezed, which causes the mouth of the flower to snap open like a dragon’s mouth. Flowers are borne on terminal long spikes of many colours except blue and with numerous shades. It is one of the excellent cut flowers which have long lasting qualities. These are also used as garden plants, bedding plants, in rockeries or herbaceous borders and as potted plants. Plants can be grown for cut flowers in an open field or under protection depending on the climate. The leaves and flowers are antiphlogistic, bitter, resolvent and stimulant (Chiej, 1984).

Carnation (Dianthus caryophyllus L.) is a member of the family Caryophyllaceae. Dianthus word was first coined by Theophrastus from Greek word “dios” meaning divine and “anthos” meaning flower. It is a half hardy, perennial plant, propagated commercially by terminal cuttings. It is being produced as a cut flower in the world. Carnation cut flower trade contributed about 15% of the world flower market. Till now, new carnation varieties have been produced mainly through conventional breeding method. The breeding procedure typically consists of hybridization, self-pollination and selection (Holly and Baker, 1992). The breeding programme did not get enough momentum due to heterozygosity, limited gene pool and inadequate knowledge of genetic make up. However genetic engineering and molecular breeding have opened the way to achieve novel colour and good vase life of flowers.

Gerbera (Gerbera jamesonii), a tender, stemless, perennial flowering plant, belongs to family Asteraceae. Gerbera was named in honour of the German botanist and naturalist “Traugott Gerber” who was a friend of Carolus Linnaeus. Gerbera is very popular and widely used as cut flower or as a decorative garden plant. It was discovered in 1878 near Barberton, eastern Transvaal (South Africa), therefore, referred as the Barberton daisy or Transvaal daisy. It is ideal for beds, borders and pots and is the fifth most important cut flower in the world.

Gladiolus (Gladiolus spp.) belongs to family Iridaceae and subfamily Ixioideae. Gladiolus is a Latin word, the diminutive of “gladius” meaning sword, sometimes called as sword lily. The plant is herbaceous perennial and semi-hardy. It is commonly grown from rounded, symmetrical corm, which is enveloped in several layers of brownish, fibrous tunics. The cultivated gladiolus is a complex assemblage of cultivars originating from complex ancestry of natural selection or hybridization involving several species. Modern garden gladiolus is a complex of at least twelve species (Raghava, 2000). It is an important commercial cut flower in both domestic and international markets.

Heliconia belongs to family Heliconiaceae is popular as ornamental plants and cut flowers because of their brilliant colours and exotic appearance. The genus name “Heliconia” is derived from “Helicon”, a mountain in southern Greece regarded by the ancient Greeks as the home of the Muses, thus suggesting the relationship between these plants and the bananas, genus Musa (Berry and Kress, 1991). Unique features of the Heliconiaceae are (1) medium to large erect herbs rising from underground rhizomes; (2) each erect shoot is composed of a stem and leaves, whereas the stem is made up of an axis covered by overlapping sheathing leaf bases, technically called a pseudostem; (3) inverted flowers and (4) the presence of a single staminode. Heliconia has long been popular horticulturally because of their showy inflorescences. It was so attractive that early explorers of the tropics returned to Europe with several species that became prized green house specimens. Heliconia, grown in nearly all of the tropical regions of the world, including Africa and Asia. In India, heliconia is grown in North-East region and Kerala.

Kangaroo Paws (Anigozanthos spp.) belongs to family Haemodoraceae. It is commonly known as Sword-like or Iris-like. The word Anigozanthos was coined by French botanist Jacques de La Billardiere. This is derived from Greek words “anoigo” meaning to “expand or unequal” and “anthos” meaning “flower or irregular corolla”. It can be used as cut flower, pot plant, landscape plants or dry flower (Gui et al., 2005). The kangaroo paws is gaining popularity among gardeners, horticulturists, nursery man also in world market due to its beautiful, unique, colourful pattern of flowers and also being perennial in nature.

Lilium is a popular genus of ornamental bulbous plants, belonging to family Liliaceae. Lilium is originated from a Greek word “li” meaning whiteness. It is an herbaceous perennial plant having scaly bulb. Despite the range of forms and their popularity as garden plants, there are two main groups of commercial importance as cut flowers i.e. Asiatic and Oriental Hybrids. They are the result of interspecific hybridization. However, intraspecific and interspecific hybridization face the hurdles like interspecific incompatibility, embryo abortion, degeneration of ovule, poor seed set and pollen sterility. There are several breeding methods and techniques which have been used to overcome these hurdles.

Lisianthus is among top ten cut flower crop of the world. In short duration of 20-30 years it become top ranking flower crop of world due to its beautiful flowers which bears beauty of different top ranking old commercial flower crops with variation in flower colours, shape, size with long vase life. It looks like tulips as they began to open and as poppies when fully opened, gaps between the petals appears like daisy, ruffled petals resemble carnations and the most important one is the flower buds and opened double type lisianthus resemble rose buds and open flower respectively and there is no doubt that this is a primary reason for consumer acceptance. Double flowered lisianthus are more demanded in U.S. as cut flower about 80% of lisianthus market whereas, single flowered are more dominant in Japanese and European markets. In Japan over 129 million cut stems were sold in 2001 placing it on number one position in Europe, over 122 million cut stems were sold in 2001 and placing it in top ten ranking and in the United States over 14 million cut stems were sold in 2002, it is not only popular as a cut flower but also as a bedding plant and a pot plant in market (Grueber et al., 1985, Halevy and Kofranek, 1984, Roh and Lawson, 1987, Tjia and Sheehan, 1984, Tija and Sheehan 1986, Skrzypczak et al., 1993 and Harbaugh, 2007), a plant bears 20-30 flowers per plant (Skrzypczak et al., 1993).

Tulip cut flower is first among the bulbous ornamentals due to its attractive colours and exquisite flowers. Tulip is a member of Liliaceae family, commonly grown from bulbs. The name tulip is derived from the Persian word “toliban” meaning “turban”, which is an appropriate term to describe the flower shape of certain tulips. The mother bulb dies and is replaced by several daughter bulbs, each of which is an annual unit of growth borne in the axils of the mother bulb scale. Tulips are basically grown for three purposes i.e. bulb production, forcing as cut flower and potted plant and for landscaping (Singh, 2006). The world tulip flower industry is dominated by the Netherlands. Another important tulip producing country is France.

Zantedeschia commonly known as Calla lily, Richardia or Pig lily belongs to family Araceae. It is the only genus in the tribe Zantedeschiae which is the sub family philodendroideae. The name Zantedeschia was given as a tribute to Italian botanist “Giovanni Zantedeschi” (1773-1846) by the German botanist Kurt Sprengel (1766-1833). It is considered by many people in the Republic of Ireland to be a symbol of Irish Republicanism. The Romans valued them so much that they often decorated the edges of the bloom with filaments of gold. These spectacular flowers are beginning to rival the rose in popularity for bridal bouquets. In India there has been initiation in the production of tubers of Calla and few tissue culture labs have started production for New Zealand and Dutch Companies. It is used as potted plants, callas are very attractive. It is used for decoration throughout the spring and early summer months focal point for a spectacular arrangement or bouquet.

China aster (Callistephus chinensis L. Nees) belongs to family Asteraceae. It is a half hardy and branching annual. Its generic name Callistephus is derived from two Greek words “kalistos” meaning beautiful and “stephos” meaning a crown alluding the large colourful flower heads. Plant is erect having hispid-hairy branches (Bailey, 1963). It is propagated through seed. It is cultivated as cut flower both in the field and under protected condition in temperate countries (Post, 1956 and Warren Auman, 1980). Whereas, in India it is widely grown in Karnataka, Tamil Nadu, West Bengal and Maharastra for cut as well as loose flower production (Janakiram et al., 2001).

Chrysanthemum (Dendranthema spp.) belongs to family Compositae (Asteraceae). It was named by Carolus Linnaeus from the Greek prefix “chrys” means golden and “anthemon” means flower. Chrysanthemum morifolium is the modern florists chrysanthemum, which evolved as a result of endless variation and hybridization from C. sinensis, C. indicum and C. ornatum (Bailey, 1963). This group is also distinguished as C. hortorum. The name of C. morifolium Ramat has been changed to Dendranthema grandiflora Tzvelev (Anderson, 1987, Heywood and Humphries, 1977). Chrysanthemums are mainly herbaceous perennial and bear flowers in capitula. Chrysanthemum perennates in nature by means of stolons and is readily propagated through basal stem cuttings. It ranks second next to roses in importance among the cut flower crops in the world.

Modern rose (Rosa × hybrida) belongs to family Rosaceae. Rose is derived from Greek word “Rhedon” means fragrance. From the ancient time its beauty and attractiveness drag the attention of breeders to create new colours and types. Present cut roses are complex hybrids developed through interspecific hybridization, polyploidy with male and female sterility and these heterozygous genotypes are propagated vegetatively. Rose occupies the first rank among the cut flowers in international market and in India it occupied first position in cut flower export trade.

Tuberose (Polianthes tuberosa L.) belonging to family Amaryllidaceae is half hardy, perennial, bulbous plant. Bulbs are made of scales and leaf bases and stem remains concealed within scales. The generic name Polianthes is derived from Greek word “polios” meaning shining or white and “anthos” meaning flower, in allusion to the blooms of the common tuberose and species tuberosa, the plant being tuberous in nature. Tuberose is commercially cultivated in tropical and sub tropical areas for cut and loose flower trade and also for the extraction of it’s highly valued natural flower oil.

Cyclamen (Cyclamen persicum Mill.), belonging to family Primulaceae, is cultivated throughout the Temperate Zone. Many people have enjoyed the charming plants as potted and/or garden plants. The cyclamen is one of the most famous and important commercial ornamental plants in many countries. It is commonly grown for its flowers, both outdoors and indoors in pots. It is used on a terrace, in flower boxes, in hanging baskets or in beddings. Several species, particularly Cyclamen hederifolium, are hardy and can be grown outdoors in mild climates such as Northwest Europe and the Pacific Northwest of North America. The cyclamen commonly sold by florists is C. persicum, which is frost-tender. Selected cyclamen cultivars can have white, bright pink, red or purple flowers. The use as cut flower is not important part of cyclamen production (Grey-Wilson, 2002), but not rare in Europe, especially in Germany. Some cultivars for cut flower production were bred in Europe, but cut flower cyclamen production is not popular in USA and Japan. Its popularity as pot plant led to many studies on the group, including cytology (Bennett and Grimshaw, 1991), hybridization (Gielly et al., 2001 and Grey-Wilson, 2002) and phenology (Debussche et al., 2004).

Freesia is ornamental flowering plant belongs to family Iridaceae subfamily Ixioideae. Freesia is named after a physician and botanist from Germany named “Friedrich H.T. Freese” (1795–1876). Dr. Christian P. Ecklon, a collector of plants from South Africa, named the flower after his friend and student. Modern freesias are known as Freesia × hybrida and cultivated for cut flowers. Freesia is grown for two purposes i.e. cut flower and pot plant.
 
It is excellent cut flowers because of its appealing shapes and found suitable for various types of arrangements and bouquets. Wide range of colour increases its beauty. Hence, its flower demand increased dramatically in recent year in global market. Forced freesias are grown as pot plant. Colours in freesias consist of pink, red, orange, yellow, blue, lavender, cream and white. Some flowers are multicoloured and possess several variations of colour within the same bud. Because of their delectable fragrance, Freesias have been drafted as a vital ingredient in many fragrance oils. Freesia fragrance oils are utilized for many oil burners and vaporizers. It is used as an essential fragrance in potpourris. Freesia essence oil is high grade essence oil. This is because it is undiluted, alcohol free, and long lasting. The oil is used for aromatherapy to scent candles, soaps, massage oils, bath oils and perfumes.

Gypsophila is herbacaeous annual or perennial flowering plant belongs to family Caryophyllaceae. It contains several ornamental species of which Gypsophila paniculata L. is the only species used as a cut flower (Zvi et al., 2008) and it is applied as filler in flower arrangements (Jones, 1995 and Rehman, 2002). Gypsophila derived from “gypson” meaning chalk and “phylos” meaning lover, an etymology which alludes to its preference for calcareous soils (Vettory et al., 2013). Mainly found in temperate or warm-temperate regions in the Northern Hemisphere, best developed in the Mediterranean region and Near East (Ataslar et al., 2009 and Cronquist, 1981). It is a perennial plant often grown commercially as an annual crop. Root is rich source of triterpine saponins (Henry, 1993, Hostettmann, 1995 and Gevrenova et al., 2010) which are used for commercially in medicines, detergents, adjuvents and cosmetics (Hostettmann, 1995, Tschesche and Wulff, 1973 and Gevrenova et al., 2010).