eChapters

Apomixis is an asexual method of reproduction through the seed. Apomictic reproduction is widespread and found in over 300 species of plants in 30 families. Interest in apomixis as a reproductive mechanism has increased as its potential and role are being recognized in genome preservation, polyploid build up, propagation of derivatives of wide crosses, permanent fixation of hybrid vigour, evolution, speciation and plant breeding .

Winkler (1906) defined apomixis as “the substitution for sexual reproduction of another asexual reproductive process that does not involve nuclear or cellular fusion.” One of the chief barriers to an understanding of the apomictic phenomenon has been the highly complex terminology as well as the differences of opinion among specialists as to the correct usage of these terms, probably because the phenomenon by itself is complex and in many ways quite different from that of amphimixis. Convincing evidence for the presence of apomixis can be obtained by tracing the steps that are involved in embryonic development. It should be amply clear that as the prerequisite for apomixis, fertilization does not take place at all in any form. Thus there is no normal development of embryo from the egg cell. However, the embryo may develop from an unfertilized egg cell other than the egg cell within the embryo sac.

Apomixis is widely distributed among higher plants. More than 300 species belonging to 35 families are apomictic. It is most common in Gramineae, Compositae, Rosaceae and Rutaceae. Among the major cereals like maize, wheat and pearl millet have apomictic relatives.

I. FILL UPS

1. Development of seed without fertilization is called _____.
2. _____ is the substitution for any sexual nuclear fusion.
3. Apomitic seed will have genetic material only from the _____ plant.
4. In _____ apomixis type, the megaspore cell undergoes the usual meiotic division and forms a haploid embryo sac.
5. The process of seed formation without sexual fusion is known as _____.
6. _____ is defined as the development of embryo from some other cell of embryo sac other than egg.
7. Haploid embryo arises from the egg without fertilization is called_____.

1. APOMIXIS

Development of seed without fertilization. When the seed formation occurs without sexual fusion, the process is known as apomixis. This occurs by several mechanism, however all apomitic seed have genetic material only from the female plant Apomixis may or may not require pollination and pollen tube germination to initiate seed formation, however sexual union.

Introduction

Apomixis is a phenomenon in which there is development of seed without fertilization or sexual fusion. Embryo develops without fertilization in apomixis. Hence, apomixis is an asexual means of reproduction therefore the genotype of plant will be same as of mother plant. Apomixis was first time discovered by Leuwenhock in 1719 in citrus whereas the term apomixis was first time used by Winker in 1908.The main benefit of apomixis over sexual reproduction is the selection of individual plant with superior characters and propagation of clonally through seeds. Higher plants commonly exhibit apomixis. There are more than 300 species belonging to 35 families that exhibit apomixis. Most commonly, it occurs in Gramineae, Compositae, Rosaceae, and Rutaceae.

5.1 APOMIXIS

Apomixis is derived from two Greek words “Apo” (away from) and “mixis” (the act of mixing or mingling). It refers to the occurrence of an asexual reproductive process in the place of normal sexual processes involving reduction division and fertilization. During sexual reproduction, developmental steps occurring inside the ovule produce the female gametophyte (embryo sac) and following a double fertilization event give rise to embryo and endosperm structures. Apomixis can be best described as the reference to the asexual process. In some species, embryo sac is produced not as a result of meiosis and fertilization but by certain asexual processes. The occurrence of such asexual reproductive process in the place of normal seed reproductive processes of reduction division and fertilization is known as Apomixis. Such seedling plants produced in this manner is known as apomicts. ’’Apomixis’’ is used synonymously with ’’agamospermy’’ (Stebbins 1950), referring to the asexual production of seed.

Apomixis (asexual seed formation) is a phenomenon in which a plant bypasses the most fundamental aspects of sexual reproduction—meiosis and fertilization—to form a viable seed. Plants can form seeds without fertilization, and the seed genotype is consistent with the female parent. The development of apomictic technology would be revolutionary for agriculture and for food production as it would reduce costs and breeding times and also avoid many complications typical of sexual reproduction (e.g., incompatibility barriers) and of vegetative propagation (e.g., viral transfer). The application of apomictic reproductive technology has the potential to revolutionize crop breeding. Apomixis is widely distributed among higher plants. More than 300 species belonging to 35 families are apomictic. It is most common in Gramineae, Compositae, Rosaceae and Rutaceae. Among the major cereals maize, wheat and pearl millet have apomictic relatives. Apomixis is reproduction where only one parent passes genes to the offspring (King, 2006). Apomictically produced offspring are genetically identical to the parent (clones).The method is mostly seen in plants, especially flowering plants. Apomixis was defined by Hans Winkler (2008) as the replacement of the normal sexual reproduction by asexual reproduction, without fertilization. Propagation from cuttings or leaves, is not apomixis. Natural replacement of the seed by an asexual method is apomixis.

A family of about 400 genera and 4,555 species of mostly tropical and subtropical distribution, but a few species distributed also in temperature regions. About 30 genera and over 60 species of Apocynaceae have been reported from India. Some of the larger genera along with their approximately reported species in bracket are Rauvolfia (100), Tabernaemontana (100), Parsonsia (100), Aspidosperma (80), Prestonia (65), Strophanthus (60), Apocynum (7), Plumeria (6) and Catharanthus (5). Some plants are of medicinal value and some are highly ornamental.
Periwinkle (syn. Vinca) is grown as an ornamental bedding plant in gardens and parks and is known for its colourful flowers and ever-blooming nature. In India, it is commonly known as sada bahar, sada phuli, and barmasi. It is one of the top ten best selling plant in USA. Its importance has increased owing to medicinal properties and as a model species for study of plant’s secondary metabolism. The plant contains more than 70 different alkaloids in leaves and roots which contribute to anti-cancerous (vincristine and vinblastine) and antihypertensive (ajamalicine) properties, respectively.

APPENDIX – 1: Basic Information

Table 1: Spacing and plant population

The researcher desires to make a study on the socio-cultural aspects of cyber spatial interactions. As you are aware, cyber space or Internet has emerged as a powerful medium of communication. The survey is meant purely for academic purpose and therefore, shall not be passed on to any one and the data obtained from the respondents shall be kept confidential for all practical purposes. The researcher sincerely requests your honest responses. The questions are of people in general, and that there is no right or wrong answers.

Appendix 1 : Syllabus (For the Degree of Bachelor Of Veterinary Science And Animal Husbandry (B.V.Sc. & A.H.)

The cattle skeleton, like that of other mammals, is a complex structure that provides support, protection, and mobility. The skeleton for study purposes is divided into two main parts: the axial skeleton and the appendicular skeleton. The axial skeleton forms the central axis of the body and includes bones such as the skull, vertebral column, ribs, and sternum. The appendicular skeleton comprises the bones of the forelimb (scapula, humerus, radius-ulna, carpals, metacarpals and phalanges) and hindlimb (os coxae, femur, patella, tibia-fibula, tarsals, metatarsals and phalanges).

Appendix - 1

Differential Diagnosis of Poisons based on Clinical Signs

1. Introduction

The cultivated apple (Malus × domestica Borkh.), is the most important fruit in temperate regions of the world. Apples were frequently used in different mythologies as symbols of immortality or reincarnation. One of the most popular stories attached to apple is that of Adam and Eve, who ate the forbidden fruit from the tree of good and evil which was in the center of the garden of Eden. The Latin word Malus has two meaning i.e. apple and evil, which has probably stemmed from this story from bible. In Greek mythology, mother earth or Gaia, presented a tree with golden apples to Zeus and his bride Hera on their wedding day. These golden apples were later indicated with many stories of love, bribery and temptation ranging from the abduction of Helen of Troy to the defeat and marriage of Atlanta. Goddess Pomona was deity of fruit trees in Rome. In many cultures, garden of paradise and pleasure is symbolized as the place with fruit-laden apple trees. Apple is popular because of its delicious taste and pleasant aroma.

Apple is the pomaceous fruit (Malus domestica) in the rose family (Rosaceae). It is one of the most widely cultivated tree fruits, and the most widely known of the many members of genus Malus that are used by humans.

The apple originated in South-West Asia which includes Asia Minor, the Caucasus, Soviet Central Asia, Himalayan India and Pakistan. It is grown extensively in the temperate region. The major apple growing countries are Australia, Argentina, Brazil, China, Canada, Denmark, Egypt, France, Gemany, USA, India, etc. In India states of Jammu and Kashmir, Himachal Pradesh and mountanous region of Uttar Pradesh are the major apple producers.

Cultivars/Varieties: The apple varieties are divided into three groups based on their maturing periods. The early season varieties grown in the states of Himachal Pradesh, Jammu & Kashmir and Uttranchal are: Tydeman’s Early(P), Irish Peach, Early Shanburry(P), Michael Molies, Benoni Fenny, Benoni Delicious, Schlomit Starkrimson, and Chaubattia Princess. The Mid season varieties are: American Mother, Red Delicious, Richared Razakwar, Starking Delicious, Vance Delicious, Jonathan(P), McIntosh(P), Top Red Lord Lam, Cox’s Orange Pippin(P), Cortland bourne(P), Red Chief, Red Gold(P), Golden Delicious(P), Oregon Spur, Queen,s Apple, Redspur, Rome Beauty, Scarlet Siberian. The late season varieties are: Golden Delicious(P), King’s Pippin, Rymer, Yellow Newton(P), American Apirouge, Buckingham(P), Winter Banana, Kerry Pippin, Granny Smith(P), Sunhari Chamure, Golden Delicious(P), Red Delicious, Ambri Baldwin, and Yellow Newton(P).

1). Scab
Diagnostic Symptoms
• Symptoms generally noticed on leaves and fruits.
• Affected leaves become twisted or puckered and have black, circular spots on their upper surface.
• On the under surface of leaves, the spots are velvety and may coalesce to cover the whole leaf surface. Severely affected leaves may turn yellow and drop.
• Scab can also infect flower stems and cause flowers to drop.
• Lesions later become sunken and brown and may have spores around their margins.
• Infected fruit become distorted and may crack, allowing entry of secondary organisms(Figure-1).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Ascomycota

Delicious and crunchy apple fruit is one of the most popular fruit favoured by health conscious, fitness freaks who believe in “health is wealth”. It is one of the popular wonderful fruit that contains a long list of essential nutrients that in the true sense are indispensable for human health. The antioxidants in apple have many health promoting and disease prevention properties; thus justifying the adage “An apple a day keeps the doctor away”. Apples help in controlling heart diseases, weight loss and cholesterol levels. Apples do not have any cholesterol instead they have fibre which reduces cholesterol by preventing reabsorption and thus are low in calories. Unpeeled apples provide plenty of nutrients which are present just under the skin. Apples are a good source of minerals like potassium, calcium, magnesium and iron, vitamins of B-complex such as riboflavin and pyridoxine (vitamin B6), vitamin C, folic acid and beta carotene. Apart from this they also contain appreciable quantities of tartaric acid that gives tart flavour to them.

Apple codling moth: Cydia (Carpocapsa) pomonella: Tortricidae 

This is an international pest and is now seen in Kashmir, Himachal Pradesh and Tamil Nadu. This is a fruit borer whose moth is silvery white with a dark band on the margin of the forewing and light brownish hind wings with a fringed margin. The moths hold their wings roof-like over the body while at rest.

Genus : Malus  
Species : domestica (Borkh.)
Family : Rosaceae 
Chromosome No. : 2n = 34, 51, 68

Like many other fruit crops, apple too is a very old crop in cultivation. Theophratus as early as third century B.C. has indicated that the Greeks and Romans knew apple culture and among the temperate fruit crops, apple is the most ubiquitous of all fruits and is extensively grown all over the world. Huge genetic variability present has permitted the better adapted types to be selected for plantation under different agro climatic conditions.  Through continuous selection both from wild population and also from hybrid seedlings, varied types or varieties have been selected, some are suitable for cultivation in warm temperate regions and others for extreme cold conditions.  This has resulted/added to the gene pool of desired types of apples enormously. At present cultivars are available destined for specific purposes like dessert, cooking, juice, cider and for large number of other uses.

Apple is a king of temperate fruit, symbol of health and premier fruit of the world. It is a deciduous temperate fruit tree and most widely grown in the world from time immemorial. In India, a large number of apple varieties were introduced in 1887 by Mr. Alexander Coutts, and his orchard is called Hillock Head in Mashobra near to Shimla. It was also introduced in Nilgoris and Bangalore, but it was destroyed by wooly aphid in 1887. At present a small cultivation is done in Nilgiris. Among the fruits, apple is having longest storage life. Apples are mostly consumed as fresh but a small part of the production is processed into juices, jellies, canned slices, wine and other products.

Origin and Distribution

An apple is originated in the Middle East/Central Asia more than 4000 years ago where its wild ancestor, Malus sieversii, is still found today. It is spreading across Europe to France; the fruit arrived in England during the time of Norman Conquest in 1066. The pioneer apple orchard in the North American continent was planted in Boston by Reverend William Blaxton in 1625. The Only North America native apples are called crab apples or common apples.

Captain R C Scot of the British army had introduced apples like Newton Pippin, King of Pippin and the Cox Orange Pippin to the Kullu valley in 1870. These apples were not popular because of their sour taste. The sweet apples were still being imported from Japan to meet the demand of Indian market. It was during a visit to America in 1915 that Samuel Stokes bought a few saplings and planted them at his Barobagh orchard in Thanedar and in the year 1926 the first apple trees bear fruit and the apples was sold and imports from Japan is stopped. The first few saplings from its, Golden Delicious of Kinnaur and Sweet Delicious of Shimla were became popular and Himachal Pradesh grew to become one of the largest producers of the fruit today. Therefore, the Himachal Pradesh is known as apple bowl of India.

It is a serious disease of apple which not only reduces the quality and size of fruit but also cause premature fruit drop and defoliation. Fruit loses its marketability due to poor quality. It also reduces storage time and cause loss up to 70%.

• Seeds stratification at 4-7°C for 60-90 days from December to February.
• Scions cannot be used for grafting at their active stage of growth.
• Meadow orcharding: 20,000-70,000 plants/ha (Super high density)
• Bitter pit of apple: Golden delicious, yellow (due to calcium) Newton.
• Internal browning: Newton
• Himachal Pradesh is known as the “Apple bowl of India”
• Flower colour: White to Pink
• Apple is the most widely grown temperate fruit in the world
• India rank 5th in apple production in the world
• Apple crop accounts for 55% of the total area and 75% of the total production of temperate fruits in the country.
• 25% to 33% of pollinating trees in India are recommended for regular cropping.
• San Jose scale was introduced in India from France in 1906.
• Apple cider-fermented wine is prepared from apples.

Smart Farming is a hi-tech and effective system of doing agriculture and growing food in a sustainable way. It is an application of implementing connected devices and innovative technologies together into agriculture. Smart Farming majorly depends on IoT thus eliminating the need of physical work of farmers and growers and thus increasing the productivity in every possible manner.

With the recent agriculture trends dependent on agriculture, Internet of Things has brought huge benefits like efficient use of water, optimization of inputs and many more. 

Abstract

The phosphorus deficiency in soil is a major problem for agricultural production as phosphorus is one of the major constituents in energy metabolism and biosynthesis of nucleic acids and cell membranes. The phosphorous present in soil is an important macronutrient for the growth and development of the plants. The phosphorus occurs in two forms in soil, organic and inorganic. The phytates (salts of phytic acid) constitute the major form of organic phosphorus other than nucleic acids, phospholipids and other compounds in soil. Phytate-phosphorus is not readily available to plants due to its chelation with metal ions forming insoluble complexes or adsorbed to soil particles. Phosphate solubilizing and phytate hydrolyzing microorganisms, bacteria and fungi, are ubiquitous in cultivated, wetland, grassland and forest soils, which play an important role in supplying soluble phosphorus to plants. Microbes hydrolyze organic forms of phosphorus by secreting phytases and acidic/alkaline phosphatases. By genetic engineering, several attempts have been made to develop transgenic plants which are able to utilize phytates as sole source of phosphorus for their growth and development. This review focuses on the applicability of microbial phytases in promoting the growth and development of various crop plants.

Biosensors are the next generation detection devices, use biological sensing elements like enzymes, nucleic acids, antibody, cells, organelles, tissues, receptors; have got varied applications in the field of drug discovery, diagnosis, biomedicine, food safety and processing, environmental monitoring, defense and security etc. Microbial biosensors are analytical device that uses naturally occurring or engineered microbial cells as the recognition elements to detect physiological changes in cells with the help of suitable physicochemical transducer.

Introduction

The rapid pace of urbanisation and abrupt growth of population in few large cities in India has become a standing menace to balanced socio-economic development of India. The rapid pace of development in cities had always attracted investors and entrepreneurs to set their enterprises leading to creation of employment opportunities in these cities. On the contrary, the lack of opportunities in rural areas has created a backwash effect leading to creation of slums in and around potential growth poles (i.e. large cities and growing cities).

1. Introduction

Aquatic algae is present in both contaminated and uncontaminated water sources. serve as essential bioindicators for evaluating water quality. Their sensitivity to pollutants and position in The significance of lower-level organisms in the food chain lies in their invaluable role in comprehending the effects of toxic substances on aquatic ecosystems. (Dwivedi & Pandey (2002). Pesticides, including herbicides, fungicides, and insecticides, play a crucial role in agriculture by utilizing forestry and animal husbandry to improve crop production. However, their indiscriminate use can lead to environmental contamination, which poses significant risks to ecosystems and human health. Pesticide contamination primarily results from surface runoff, emissions during spraying, improper transportation, inadequate spraying practices, and insufficient wastewater treatment from the pesticide industry. Introduction of pesticides into the environment Presents numerous environmental and health risks., inhibiting plant and animal growth and affecting the development and nervous systems of animals Pesticides have the potential to infiltrate the food chain, bioaccumulate in higher trophic levels, and reach the human body through biomagnification. Commonly used pesticides, such as imidacloprid, isoproturon, simazine, atrazine, mecoprop, and glyphosate, along with historical organochlorine pesticides (OCPs), contribute to significant pesticide contamination.

Introduction

Statistics generally refers to information about an activity or a process that is expressed in quantitate as well as qualitative traits. The quantitative research process is well structured whereas the qualitative one is fairly unstructured. It depends upon how a piece of information have been collected and analyzed. A good researchers need to have both types of skill. This article highlights about the operational procedure, about methods, procedures and techniques that are used in analysis of climate related data.

Parameter vs Statistic

When a data is collected with survey of all the individuals (like in a census) and analysed for certain values, it is called a parameter. But when, we guess the same value with a random sample and interpret for whole population it is called a statistic. Environmental parameters are: mean, variance, correlation, regression, heritability, repeatability etc. Again, there are two types of variables: quantitative and qualitative. Quantitative variables can take any value in a specified range. So, they are of continuous type. But qualitative variables are very few types ( 2 ,3 or 4 types) and they are of discrete type. Body weight is an example of quantitative variable whereas, comb type is a qualitative variable.

Good livestock welfare practices demands thorough understanding of the basic nature of the animal. The science of understanding the details of animal behaviour is known as Ethology.  Knowledge about the behaviour of the species being reared proved as an important tool for implementing animal welfare in practical husbandry practices. Now a day’s global community is also advocating for the better livestock welfare standards. Exploiting animal’s natural behaviour could certainly prevent unnecessary sufferings related to handling and restraining during routine farm activities. On the one hand this ensures physical and mental welfare of livestock, while on the other hand improvement in the production is guaranteed. Without involving heavy investment behavioural knowledge based management interventions can bring paradigm shift in welfare status of farm. Therefore, behavioural studies are of great importance in increasing our understanding and appreciation of animals.

Biofertilizers are preparations containing micro-organisms, with capability of mobilizing nutritive elements from non usable form to usable form through biological processes. Bio-fertilizers have definite advantage over chemical fertilizers. Chemical fertilizers supply over nitrogen whereas bio-fertilizers provide in addition to nitrogen, certain growth promoting substances like hormones, vitamins, amino acids, etc, crops have to be provided with chemical fertilizers repeatedly to replenish the loss of nitrogen utilized for crop growth. Bio-fertilizers application in agriculture will have greater impact on organic agriculture and also on the control of environmental pollution, soil health improvement and reduction in input use. Farmers try to increase yields of vegetable crops by mean of heavy nutrition’s. The use of chemical nitrogen and phosphorus fertilizers at high levels had an adverse effect on the accumulation of NH4+, NO3-, NO2- and PO4- in vegetable product tissues. Therefore, clean agriculture recently depends upon using biofertilizers as well as organic in order to produce high yields with the best commodity quality without contamination and less accumulation with heavy metals.

The application of high input technologies such as chemical fertilizers, pesticides, herbicides have improved the production but there is growing concern over the adverse effects of the use of chemicals on soil productivity and environment quality. Thus, integrated nutrient management has become an accepted strategy to bring about improvement in soil fertility and protecting the environment. This strategy utilizes a judicious combination of fertilizers, organic manures and bio-fertilizers. Biofertilizer is a natural product carrying living microorganisms derived from the root or cultivated soil. So they don’t have any ill effect on soil health and environment. Besides their role in atmospheric nitrogen fixation and phosphorous solubilisation, these also help in stimulating the plant growth hormones providing better nutrient uptake and increased tolerance towards drought and moisture stress. A small dose of biofertilizer is sufficient to produce desirable results because each gram of carrier of bio-fertilizers contains at least 10 million viable cells of a specific strain. Biofertilizers means the product containing carrier base (solid or liquid)

Abstract

The study of palm genomics can aid to understand the genetic and molecular knowhow of all biological processes in palms that are vital tothe palm species. This knowledge will allow the path for the fruitful exploitation of palms as biological resources in the development of new cultivars of improved quality and with shortened economic and environmental costs. This knowledge is also vital for the development of new diagnostic tools. Traits considered of primary interest are resistance to pathogens and abiotic stress, fruit quality and yield. Genomics and bioinformatics approaches helped in unraveling the genomes of economicpalsm like coconut, oilpalm and datepalm. The genome characteristics and tools for genome analysis are provided in the chapter. Due to the economic importance of cocoa, many scientific research studies (genome, EST, transcriptome) have been carried out in the past years in this crop also.

Introduction

Plantation crops constitute a large group of crops. The major plantation crops include coconut, arecanut, oil palm, cashew, tea, coffee and rubber; and the minor plantation crops include cocoa (Prapulla and Indira, 2014). India is the third largest producer of coconut and goes in front of the ninety coconut-producing countries of the world (Richard 2013). India is the largest producer and consumer of cashew nuts (Mohod et al., 2011.). India also occupies number one position in arecanut production (Dwivedi et al.,2008) besides being the largest tea producers in the world. While Coffee is the second largest traded commodity in the world, it has, in recent times become an extremely important foreign exchange (Prapulla and Indira, 2014).

Abstract

Biosurfactants (BS) are amphiphilic compounds produced by microorganisms mainly bacteria and are known to contain hydrophobic and hydrophilic moieties that play a major role in reducing surface tension (ST) and interfacial tensions between individual molecules. Chemically, BS is a complex molecules consisting of lipopeptides, glycolipids, polysaccharide protein complex, fatty acids and phospholipids. They are known for their biodegradability, low toxicity, and renewable nature.

Introduction

Under the present scenario of growing human population and shrinking land and water resources for agriculture, expanding abiotic and biotic stresses, increasing genetic erosion; relentless efforts are needed to enhance the plant productivity as well as quality of produce. In fact, improvements in agricultural production as well as food and nutrition conditions depend on land, water and energy resources, which are, undoubtedly, limited and depleting at a rapid pace.

Biotechnological innovations in conjunction with conventional technologies have opened new frontiers in agriculture thereby offering exciting opportunities for increased sustainability and profitability in agriculture. Thus, biotechnological tools complement (rather than replace) the traditional methods used to enhance the agricultural productivity to cater to the needs of third world agriculture (Swarup and Srivastava1990, Rao et al. 1996).

Plant diseases are a threat to world agriculture. Significant yield losses due to the attack of pathogen occur in most of the agricultural and horticultural crop species. More than 70% of all major crop diseases are caused by fungi . Plant diseases are usually handled with applications of chemicals. For some diseases, chemical control is very effective; but it is often non-specific in its effects, killing beneficial organisms as well as pathogens. Chemical control may have undesirable effects on health, safety and cause environmental risks. A promising method for protecting plants against diseases is constructing and employing pathogen-resistant cultivars. Although a number of resistant cultivars have been developed through breeding programs, these cultivars become obsolete in a short time due to the rapid evolution of the phytopathogens and the emergence of virulent forms capable to overcome the plant resistance. Breeders are often confronted with the issue of using a limited number of plants in their breeding programs, undesirable traits transferred together with the valuable resistance genes, and, in recent years, also with the depletion of potential gene sources. Control of diseases is a subject of great interest for biotechnologists. The most significant development in the area of varietal development for disease resistance is the use of the techniques of gene isolation and genetic transformation to develop transgenic resistance to fungal, bacterial and viral diseases. Improvements in genetic transformation technology have allowed the genetic modification of almost all important food crops like rice, wheat, maize, mustard, pulses and fruits. Genetic engineering technology has proved to be beneficial in managing viral and bacterial diseases in plants. The advances in gene engineering technologies and the understanding of the molecular nature of plant protection mechanisms have provided means for developing principally new strategies of plant disease control, in addition to the traditional approaches based on employing chemicals or classical breeding schemes. Biotechnology will enhance our understanding of the mechanisms that control plant’s ability to recognize and defend itself against disease caused by fungi. The integration of biotechnology with traditional agricultural practices will be the backbone for sustainable agriculture.

Mushrooming population, subsiding agricultural resources; land and water, people’s concern towards quality of vegetable s/food and changing climate throws several challenges for vegetable production. Conventional management and crop improvement strategies alone can no longer sustain the nation’s as well as global demand. So there is an urgent need to adopt modern biotechnological methods which are multidisciplinary where a large number of tools and elements of conventional breeding technique, genetic engineering, molecular biology, molecular genetics, bioinformatics and biochemistry are utilized and integrated. Biotechnological tools provide three major aspects of genetic improvement viz, application of tissue culture, molecular breeding or marker assisted breeding and genetic engineering.

Breeds are the result of the hundreds of years of natural selection and evolution. They are adapted to tropical climate and diseases and perform reasonably well even with low quality of feed and fodder. India has agrarian economy, with majority of population dependent on agriculture. India is a mega diversity hub of different livestock breeds. There are 10 buffalo breeds, 30 cattle breeds, 42 sheep breeds, 20 goat breeds, and 18 poultry breeds contributing significantly to GDP of the country. Despite our vast population of different species, there is a need for conservation of breeds. Conservation is the management of human use of the biosphere, so that it may yield the maximum sustainable benefits to the present generation, as well as maintaining its potential to meet the needs and aspirations of future generation.

Introduction

Cell and tissue culture techniques are becoming increasingly popular as alternative means of plant vegetative propagation. Plant tissue culture involves asexual methods of propagation and its primary goal is crop improvement. The success of many in vitro selection and genetic manipulation techniques in higher plants depends on the success of in vitro plant regeneration. Crop breeding and rDNA technology require the widespread use of reliable true to type propagation and better regeneration methods. Plant regeneration can be grouped in to different categories of enhanced release of axillary bud proliferation, organogenesis and embryogenesis. The oldest practical application of tissue culture techniques is the propagation of disease free plants from meristem tip culture. Meristem tip consisting of dome and one or two subjacent leaf primordial with a size up to 0.25 mm are aseptically cultured in appropriate nutrition media. The resulting plants are disease free because the pathogen is not generally present in the meristematic cells.

The aim of germplasm conservation is to ensure the availability of useful germplasm at any time. Breeding programs rely heavily on locally adapted ancient plant varieties and their wild relatives as sources of germplasm. Further, the continuing search for high yielding varieties of crop plants with resistance to pathogens and pests warrants the availability and maintenance of large collections of germplasm. In species which are propagated through seeds, it is economical to preserve the seeds. These seeds are dried to water content of 5 - 8% and then stored at a temperature of -18° C or lower and low humidity. In case of vegetatively propagated species, preservation of germplasm heavily taxes manpower and land resources. As in vitro techniques are becoming more important in crop improvement through the use of somatic cell genetics, the material produced in vitro will have to be conserved in vitro. The in vitro system is extremely suitable for storage of plant material, since in principle it can be stored on a small scale, disease free and under conditions that limit growth. Germplasm storage in vitro is crucial for the future development and safety of agriculture.

Application of computers in Hospital Pharmacy Practice
Computers have significantly transformed hospital pharmacy practices, enhancing various aspects of patient care, medication safety, and operational efficiency. Below are the key applications of computers in this field
1. Electronic Health Records (EHRs): EHRs are comprehensive digital records that replace traditional paper charts, encompassing a patient’s entire medical history. These records include data on diagnoses, lab results, medications, immunization records, treatment plans, and demographic information. EHRs offer real-time, patient-centered records accessible to authorized healthcare providers, enabling seamless sharing of information across different departments and facilities. This enhances collaboration, leading to more accurate diagnoses, coordinated care, and effective treatment plans. Additionally, EHRs support clinical decision-making through integrated alerts and reminders, ensuring critical data is communicated promptly for better patient outcomes.
2. Clinical Decision Support Systems (CDSS): Clinical Decision Support Systems (CDSS) are advanced tools integrated within healthcare IT systems to aid healthcare professionals in decision-making processes. CDSSs analyze patient-specific data and match it with extensive medical knowledge bases to provide tailored recommendations for diagnosis and treatment options. By flagging potential issues such as drug interactions, allergies, and contraindications, these systems reduce the risk of errors and enhance patient safety. CDSS can also offer reminders for preventive measures, suggest evidence-based protocols, and guide adherence to clinical guidelines, thus streamlining the clinical workflow and improving quality of care.

Purpose-To now the technique of application of thick layer sterile absorbent protective cotton padding over fractured site and its retention with thread ligature in order to protect the bone from further damage

Site-Generally area joint above and joint below of fractured site of bone needs to be covered with a thick layer for perfect immobilization of fractured bone.

Appropriate selection of fungicides and application at appropriate doses and times are of great importance in the management of crop diseases. A basic requirement of the application method is to get the fungicide to a location where the active ingredient prevents fungal damage to the plant. The fungicidal application varies according to the nature of the host part diseased and nature of survival and spread of the pathogen. The method which are commonly adopted in the application of the fungicides are discussed.

1. Foliar or vegetative application

2. Soil application

3. Seed treatment

Pre-harvest factors are responsible for the majority of post-harvest losses. Fruits which have already been infected with insects and pathogens, have been poorly irrigated and fertilized, or are of low quality before harvesting will never benefit with post-harvest cures. As a result, the process of accomplishing production quality, as well as harvesting, processing, and selling, must be taken into account.

Embryo Transfer (ET) refers to the technique by which embryos are collected from the reproductive tract of female (donor) and transferred to that of another female (recipient) which carries it to term. The donor and recipient female are in same stage of estrous cycle. The main objective of ET is the improvement of animal population through maximum utilization of superior females and males. It allows faster progeny testing of both male and females. By obtaining and transferring embryos from pre-pubertal females shorten the generation interval from birth to reproductive age. The superior buffaloes will be sent for slaughter after getting lactation yield in metropolitan cities. Utilizing oocytes for In-vitro maturation (IVM) and In-vitro fertilization (IVF) from such slaughtered superior buffaloes can prevent wastage of genetic material. ET allows preservation of rare and extinct species and breeds of animal. ET can be used as a basic tool for twinning, cloning, sexing and to test chromosomal aberrations.

Abstract

A majority of the plants studied in natural ecosystems are symbiotic with microorganisms that either reside entirely (endophytes) or partially within plants. These microorganisms express different associations ranging from mutualism to parasitism. These symbiotic relationships appear to impart tolerance to various types of abiotic stresses such as heat, drought, salinity, heavy metals, etc. and sometimes may be responsible for the survival of both plant hosts and microbial symbionts in high stress habitats. The amelioration of the abiotic stresses by the endophytes assumes increasing significance in the light of rapidly changing global climate, which is likely to face frequent incidences of extreme weather conditions like high temperature, droughts, etc.

Introduction
The conceptual foundation of environmental DNA (eDNA) can be traced back to French criminologist Edmond Locard’s principle that “every contact leaves a trace.” eDNA, often referred to as the “blueprint of life,” represents the genetic material shed by organisms into their environment. This innovative approach allows scientists to obtain biological information without directly interacting with or disturbing the organisms. eDNA technology is particularly advantageous in studying megadiverse ecosystems, as it enables the isolation and analysis of genetic material from various environmental samples, such as soil, water, and air. The approach of environmental DNA (eDNA) has its roots in metagenomics, which has long been used to study microbial communities
in soil, water, and sediment. The concept of eDNA was first applied in microbiological research on marine environments (Orgam et al., 1987; Handelsman, 2004). Early breakthroughs in biodiversity monitoring included the detection of invasive species, such as Rana catesbeiana (American bullfrog), in French swamps, demonstrating the potential of eDNA in ecological studies
(Díaz-Ferguson and Moyer, 2014). The persistence of eDNA also depends on the species and habitat: in freshwater, it may last between 15 and 30 days for fish and amphibians, while in marine environments, it typically persists for shorter durations, ranging from 0.9 to 7 days for marine mammals. These variations underscore the importance of understanding environmental and biological factors when applying eDNA methods for monitoring biodiversity and ecosystem health. The transport of eDNA in rivers is particularly rapid, with genetic material potentially traveling tens of kilometers from its source. This dynamic propagation must be accounted for when designing experiments and interpreting results.

1. INTRODUCTION

‘Fertigation’ is the technique of supplying dissolved fertilizer to crops through an irrigation system (Haynes, 1985). When efficient irrigation system combined with both nutrients and water, it can lead to obtain higher yield and preferable quality desired marketable produce.

Often, side-dressings of straight solid fertilizers are found to be the management constraints. Most of the growers have the experience of how much the applied fertilizer is either washed from the soil in run-off or leached below the root zone.

Continuous small applications of soluble nutrients have the benefits like labour, due to fertilizer being placed uniformly around the plant roots and allow savings/economy rapid uptake of nutrients by the plant.

Aim: To know the technique of application of first layer padded cloth and its thread

Key words : Integrated Watershed Management (IWM), GIS, DTM, Watershed Delination, Water Quality Modeling, RS, DSS, Ground Water Information System (GWIS).

Introduction

The availability of land and water resources has a major effect on the economic and ecological aspects of life everywhere around the world. As a result, the need to develop better tools for efficient land and water resources management has become very vital. For quicker, reliable and precise results, it is necessary to use the geospatial tools and techniques such as Global Positioning Systems (GPS), Geographic Information Systems (GIS) and Remote Sensing (RS) image interpretation and analysis techniques to manage the natural resources. The application of these tools and techniques not only facilitates timely and repetitive analysis of all the topological features, but also assists in development of Decision Support Systems (DSS) to generate alternative scenarios for optimal use of the natural resources in the long term. The optimal use of these resources through integrated management of land water and vegetation leads to enhanced production and productivity of food, fodder, fiber, fuel, fish, flower and forest resources. Moreover, the holistic management of the resources to produce these outputs is plausible and manageable within the watershed systems, which are hydro geological units from which the runoff resulting from precipitation flows past through a single outlet to downstream locations. The systematic management of land, water, vegetation and livestock resources within the watershed systems is termed as Integrated Watershed Management (IWM). Broadly, IWM can be defined as a multidisciplinary, holistic way of protecting and managing a watershed’s natural resources to enhance biomass production in an eco-friendly manner. The watershed is viewed as a hydro-geological complex and dynamic ecosystem in which natural and anthropogenic processes occur and interact resulting in generation of runoff at the watershed outlet.

Abstract

This study was conducted at Directorate of Cashew Research (DCR) Puttur, D.K., Karnataka during 2007-12. In this study, a methodology was standardized to find the area under cashew, barren areas and other areas for expanding cashew cultivation with remote sensing. It was found that the images pertaining to the month of November was best for classifying cashew since the colour of cashew leaves changes during that period. Also, the LISS III images were found to be good for identifying cashew. It was not possible to differentiate between cashew seedling area and cashew graft area using LISS III or LISS IV images. A spatial database of soil moisture was prepared for the irrigation scheduling in cashew and a spatial database of major nutrients was prepared for the site specific nutrient management in cashew gardens. A spatial database of land slope and stream orders was prepared using GIS and suitable soil and water conservation interventions were suggested for the selected area.

Keywords: Cashew, GIS, remote sensing

Introduction

Natural Disasters are inevitable, and Indian subcontinent is prone to all type of Natural Disaster either it is flood, drought, cyclone, earthquakes, tsunami or forest fires etc. Disasters can be classified in several ways. A possible sub-division of disaster is: (i) Natural Disasters; (ii) Human made disasters; (iii) Human induced disasters. Another sub-division is related to the main controlling factors leading to a disaster. These may be meteorological, geomorphological/geological, ecological, technological, global environmental and extra terrestrial. Another useful distinction that can be made between disasters is regarding their duration of impact and the time of forewarning. Some disaster strikes within a short period with devastating outcomes; others have a slow onset period with equally or more serious repercussions. According to the available statistics 60% of the total area of Indian subcontinent is venerable to seismic activity of varying intensities, 16% of the country’s total area is drought prone. In India 68% of total sown area of the country is drought prone. Coastal areas of India is exposed to tropical cyclones, tsunami in recent years and among all the disaster occur in the country, River floods are the most frequent and often the most devastating.

ABSTRACT

Dairy Development plays a vital role in bringing out significant changes in socio-economic structure of rural economy. The main objectives of Dairy Developments in India is to increase production and availability of milk through integrated policy of cattle cum Dairy Development, eliminating the middlemen, through formation of co-operatives and self supporting dairy enterprise. The advent of Geographical Information System (GIS) has added new vistas in the field of Dairy Farm and Management. It also helps in integrating the whole Dairy Farm in a more precise way to get correct information about the various factors related with the whole dairy farm.

Introduction

The farm of Sarasa Green Projects around Chapabandi, Bansgora and Hetedoba Mouzas near Durgapur in Burdwan District of West-Bengal. Dairy Development plays a vital roll in brining about significant changes in socio-economic structure of rural economy. The main objectives of Dairy development in West-Bengal is to increase production and availability of milk through integrated policy of cattle cum Dairy Development eliminating the middlemen through formation of co-operatives and self supporting dairy enterprise. (Fig.1)