eChapters

Silver Staining for Protein

Silver staining is one of the procedures, in addition to Coomassie Blue, R and G types, and fluorescent dyes that are available for detecting proteins separated by gel electrophoresis. Switzer introduced silver staining in 1979, a technique that today provides a very sensitive tool for protein visualization with a detection level down to the 0.3-10 ng level.

The basic mechanisms underlying silver staining of proteins in gels are relatively well understood. Basically, protein detection depends on the binding of silver ions to the amino acid side chains, primary the sulfhydril and carboxiyl groups of proteins, followed by reduction to free metallic silver. The protein bands are visualized as spots where the reduction occurs and, as a result, the image of protein distribution within the gel is based on the difference in oxidation-reduction potential between the gel’s area occupied by proteins and the free adjacent sites. A number of alteration in the silver staining procedure can shift the oxidation-reduction equilibrium in a way that gel- separated proteins will be visualized either as positively or negatively stained bands. Silver staining protocols can be divided into two general categories: 1) silver amine or alkaline methods, and 2) silver nitrate or acidic methods. In general, the detection levels’ using the various procedures is determined by how quickly the protein bands develop in relationship to the background (e.g. signal-to-noise ratio). The silver amine or alkaline methods usually have lower background and as a result are most sensitive but require longer procedures. Acidic protocols, on the other hand, are faster but slightly less sensitive. Clearly, each protocol has different advantages regarding timing, sensitivity, cost, and compatibility with other analytical methods, especially mass-spectrometry (MS) a tool that is being used in combination with gel electrophoresis or chromatographic methods for rapid protein identification. Until recently most of the silver staining protocols used either glutaraldehyde- of formaldehyde-based sensitizes in the fixing and sensitization step, thus, introducing the chemical modifications into proteins. The utilization of those chemicals causes the cross-linking of two lysine residues within protein chain, thus, strongly affecting two most crucial steps for the subsequent MS analysis: hampering the trypsin digestion and highly reduces the protein extraction from the gel .

21.1. Aim

To familiarize the students with the machineries and equipments used in fruit and vegetable processing.

21.2. Theory

The students will visit the processing unit/pilot plant and familiarize with different machineries and equipment used in fruit processing units. The students will observe the layout and the details of the machinery for different unit operations viz; raw material receiving, washing, sorting/grading, peeling, slicing, pulping, heating, filling, exhausting, sealing, sterilization/processing, labelling and storage for different products. The list of the machineries and equipments and important chemicals used in fruit preservation is as under:

21.3. Apparatus/machineries/equipments used in food industries

21.3.1. Basic equipment/ accessories

Introduction

Indian economy is basically an agrarian economy. In spite of all challenges and difficulties faced over the years, the sector still serves as the lifeblood of the nation, by standing as the major source of livelihood for over half of the population by guaranteeing food security for its teeming millions. A significant contribution to the GDP of the nation is still ensured by the agriculture sector. The development of a nation highly centres on its primary agriculture sector and Indian economy have a much diverse dependence on the agriculture sector in terms of its growth and development. The nation straightaway necessitates a tremendous shift entirely to sustainable agricultural practices in its development trajectory.

Despite its undeniable significance and praised role, Indian agriculture currently faces multifaceted challenges, which significantly restrict the production and productivity level of the sector as a whole and the farmers in particular. Its high time that the Indian agriculture sector, which stands as the backbone of the nation needs to go through a looming transformation. The industry 4.0 revolution is a vibrant platform for the sector to make a complete transformation. The traditional face of the sector will transform towards a modern makeover by taking up technological advancement and digitalisation. The wider transformative opportunities will ensure a return of the Indian agriculture sector to the forefront in the nations growth and development.

Introduction
Chromosome engineering has emerged as a revolutionary approach, offering immense potential for genetic improvement by manipulating the structure and composition of chromosomes. Using genome engineering technologies, it is possible to introduce desirable traits into plants, such as disease resistance, increased yield, and enhanced nutritional content. To sustain the current standard of living, agriculture must yield significant increase in production (Gerland et al. 2014). Novel cutting-edge techniques developed for chromosome engineering hold great promise for addressing the challenges faced by modern agriculture, including climate change and food security. Engineering ability to precisely modify the genetic makeup of plants, chromosome engineering opens new avenues for creating novel cultivars with improved characteristics. While classical breeding has been successful in developing improved cultivars, it has its limitations. The process is time-consuming and often relies on probability to bring desired genetic changes. Since genes are organized into chromosomes, one important step to solve this issue is the development of novel genetic tools for chromosome reorganization and manipulation. When (Sears 1956) used X-ray irradiation to introduce foreign chromosome segments into the wheat chromosomes to transfer new traits, it was the beginning of plant chromosome engineering. Since then, plant chromosome engineering has evolved.
Applying methods such as chemicals and X-rays, induced mutagenesis was essential to the Green Revolution. Induction of polyploidy yielded more vigor and benefits like larger and higher-quality fruits (Osborn et al. 2003). Insect resistant and herbicide resistant transgenic crops successfully contributed to enhanced food production with less input into the farms. Genome editing enables precise modifications to increase disease resistance and stress tolerance (Ma et al. 2015). Considering practical features of chromosome engineering, this article discusses the use of induced mutagenesis, polyploidy induction, transgenesis, and genome editing, as the practical tools. The purpose of this chapter is to provide an in-depth analysis of chromosome engineering, exploring its scope, historical milestones, techniques, and its contributions to genetic diversity improvement and crop improvement. It also highlights the importance of efficient delivery techniques to overcome constraints such as off-target effects and concerns about genetically modified organisms (GMOs).

Introduction
According to the U.S. Food and Drug Administration (FDA), Good Laboratory Practices (GLP) is a quality system that ensures the nonclinical safety studies are appropriately designed, conducted, and documented to generate reliable data. The laboratory techniques are defined as the processes and practices that are recommended for equipment use in the laboratory, while laboratory methods are based on established scientific principles involving biology, chemistry, and physics, and encompass all aspects of the clinical laboratory from testing the amount of cholesterol in blood to analysing DNA to growing microscopic organisms that may be causing an infection. One of the most significant parts of laboratory techniques and laboratory methods is the proper validation of the equipments (old or new equipments) as well as all the testing procedures/ methods (established or newly developed methods). Analytical method validation is a critical process to ensure the quality and safety of products. The objective of the validation of an analytical method is to demonstrate that it is suitable for its intended purpose; the results are authentic and universally acceptable. An analytical method is suitably well considered when the results generated through different method are consistent, reproducible, and thus, reliable.
The validation is required when
• A new method has been developed
• Revision of established method
• Use of established methods in different laboratories and different analysts, etc.
• Comparison of methods required
• When quality control indicates method changes

Dairy rearing is central to livelihoods and survival of millions of people of India. It is estimated that approximately 100 million people derive their live hood from dairy rearing as primary or secondary source of income. Dairy related activities help to maintain regular in flow of income for these households. Small farmers in India generate nearly half of their income from dairy and the value of cattle represents a significant part of their wealth, so the death of cattle poses a significant risk and affects farmers’ net worth and income.

a. Type of risks to dairy based livelihoods

Large animal’s are expensive and thus carry higher risk exposure. Dairy losses due to the disease, accident, theft, natural calamity can cause significant losses to these household. Sometimes owners are forced to sell their animals due to loss due to incurable disease/ disorder/ fodder and water scarcity and they are unable to rebuild their stock. Many times animal losses force them into poverty trap from where they are unable to recover.

Risks faced by dairy owner’s dairy dependent livelihoods can be classified into two broad categories

2.1 Setting Business Goals and Objectives

Well-chosen goals and objectives point a new business in the right direction and keep an established company on the right track. When establishing goals and objectives, try to involve everyone who will have the responsibility of achieving those goals and objectives after you lay them out. To help you better understand how you can set goals and objectives, you first need a good foundation for what the two are.

Goals establish where you intend to go and tell you when you get there. They help improve your overall effectiveness as a company — whether you want to increase your share of the market, for example, or improve your customer service. The more carefully you define your goals, the more likely you are to do the right things and achieve what you wanted to accomplish in the first place.

Objectives are the specific steps you and your company need to take in order to reach each of your goals. They specify what you must do — and when.

Deoxyribonucleic acid (DNA) is a molecule made up of pairs of building blocks called nucleotides. The four kinds of nucleotides that make up DNA are adenine (abbreviated as the single letter A), guanine (G), cytosine (C), and thymine (T). The DNA molecule has the shape of two intertwined spirals, referred to as a double helix. DNA is packaged into chromosomes that are located within the nucleus of all cells. These chromosomes are the same in every cell of an organism and together make up the organism’s genetic information, its genome. Chromosomes contain stretches of DNA called genes that code for amino acids that make proteins. It is the proteins that are the foundation of life for all organisms. The interaction and structure of proteins determine the visible characteristics or phenotype of an organism, while the genetic makeup of an organism is called its genotype.

The sequence of nucleotides that make up a gene can differ among individuals. The different forms of a gene are called alleles. The alleles are the result of nucleotide differences in a gene that affect an amino acid sequence of a protein. This can result in a change, addition, or deletion of a protein that can affect the phenotype.

English name : Fennel
Botanical name : Foeniculum vulgare Miller
Family : Apiaceae
Chromosome number : 2n = 22
Centre of origin : Southern Europe, Asia and Africa
Fennel (Foeniculum vulgare Miller), commonly known as Saunf or Badi Saunf, belongs to the Apiaceae family and is primarily cultivated for its seeds, from which fennel oil is extracted. The main component of fennel oil from cultivated F. vulgare is trans-anethole, which typically makes up 50-60% of the oil. Other constituents include d-2 fenchone, methyl chavicol, 2-pinene, camphene, d-2 phellandrene, dipentenes, B-pinene, terpinene, limonene, 1,8-cineole, a-terpinene, linalool, cis-anethole, anisic aldehyde, anisic ketone, and foenicullin. Recently, the presence of a toxic ketonic constituent has been reported. Indian fennel oil contains over 70% anethole and around 6% fenchone. Sweet or Roman fennel fruits are rich in anethole, phellandrene, and d-limonene, with a high concentration of anethole (up to 90%) and the absence of fenchone contributing to its sweet fragrance and flavour. Anisaldehyde and anisic acid have also been identified as present.

This chapter introduces continuous cover forestry as an approach to managing forest ecosystems while maintaining relatively continuous canopy cover and ecological processes. It examines the concept and evolution of continuous cover forestry, its ecological foundations, and advanced silvicultural practices. The chapter considers its ecological, economic, and social benefits and discusses its relevance to climate change adaptation and sustainable forest management. Comparative perspectives with conventional forestry, global case studies, challenges, limitations, and future prospects are also addressed. The approach emphasizes maintaining forest structure, regeneration, biodiversity, and ecosystem functions while allowing appropriate harvesting and resource utilization.

Bioinformatics is the application of information technology to the field of molecular biology. The term Bioinformatics was coined by Paulien Hogewag, in 1978 for the study of informatic processes in biotic systems. Bioinformatics nowadays, entails the creation and advancement of databases, algorithms, computational and statistical techniques, and theory to solve formal and practical problems arising from the management and analysis of biological data. Bioinformatics deals with storing and searching of large biological data sets and analysis of these data sets using computers to determine various relationships. 
The rapid developments in genomic and other molecular research technologies and developments in information technologies have been combined to produce tremendous amount of information related to molecular biologists. Now bioinformatics has developed and deals with many other types of biological data like protein structures, sequence alignment, gene finding, genome assembly, gene expression profiles, structure alignment, protein structure prediction, protein–protein interactions and the modelling of evolution. Being an interface between modern biology and informatics, it involves detection, expansion and implementation of computational algorithms and some of the software tools used to provide applications primarily in agriculture and healthcare divisions. In developing countries, bioinformatics play a major role in agricultural area where it can be used for increasing the nutritional content, and increasing the volume of the agricultural products etc.

ABSTRACT

The paper discusses distribution of bamboo, their uses and bamboo based agroforestry systems in India and their future potential. Bamboo, one of the fastest growing plant species is extremely versatile and has been traditionally put to a large number of uses. Application of modern technology and industrial processing has catapulted bamboo into a new global limelight. At present there is huge demand of bamboo based raw material viz., timber, plywood, bamboo flooring, edible bamboo shoots, etc. in the international market and most of the international market of bamboo is captured by China. In India, most of the bamboo raw material comes from natural forest. To capture the emerging bamboo market we have to increase the bamboo cultivation outside the forest under different bamboo based agroforestry systems. Experiments have shown that bamboo based agrisilviculture systems is technically and economically feasible in our country under different agro-ecological conditions. The agriculture crops which can be profitably grown with bamboo are soybean, pigeon pea, ginger, turmeric, etc. Keeping in view the international scenario, ecological conditions and taking clues from China’s success stories, some of the bamboo based agroforestry system components are also proposed.

Introduction

A disaster is the result of an immediate situation or the result of a long, set process, which disrupts normal human life in its established social, traditional and economic system. This is due to the destruction of environment which is caused by extraordinary natural destructive phenomena or human-induced hazards resulting in human hardship and suffering beyond recovery unless external aid is brought in.

1. Mendel presented his results in the form of

   a) Two paper in French langua

   b) Two paper in German language

   c) One paper in German language

   d) None of the above

2. The alternative forms of a gene are called

   a) Allelomorph

   b) Gene

   c) Alleles

   d) None of these

8.1 Matrix

An arrangement of m×n elements into m rows and n columns is called a matrix. A matrix is a rectangular array of numbers usually represented by enclosing the array in brackets.

Order of a Matrix : The number of rows and columns is called the ‘dimension’ or ‘order’ of the matrix.

Introduction

Musculoskeletal diseases in poultry are often exhibited as lameness or leg weakness. Numerous factors, including those related to diet, animal husbandry, infections, or heredity, might contribute to musculoskeletal problems. Lameness can be exhibited due to disorders in the central or peripheral neurological systems, reproductive system, trauma or infection in the foot, leg, hip, spine, or just a generalised sickness. Less frequently, musculoskeletal anomalies can affect the bird’s beak, neck, or wings. The diseases can also be classified as infectious origin (bacterial, viral) or non-infectious origin (congenital deformities, nutritional, trauma or neoplasia). Modern poultry production lines put a lot of strain on the musculoskeletal system due to factors like growth rate in broiler chickens and egg output in laying hens. As a result, poor nutrition and husbandry practices frequently lead to musculoskeletal disorders. Thus, musculoskeletal disorders in poultry can be controlled by choosing proper genetic selection, efficient vaccinations, adequate nutrition and proper husbandry practices.

In the last several decades many international forums and institutions have discussed and agreed that food and water security are the most important issues for the future. The damage to the ecology directly affects the food, land, water, health and the livelihood. Agricultural progress serves as the best way to fight poverty, hunger and livelihood problems. Though in the last few decades, billions of pages about hunger, food and water security have been written, thousands of conferences have been organized, the urgency of action plans and creating holistic models is the most important need. For this, we must require bold reforms and action oriented programs which can reach the smallest farmers and give an optimistic signal rather than a despondent picture. Developmental models that are holistic and close to nature, sustainable and based on inclusiveness considering ‘unto the last’ women and man are the need of the day. Land and water together with biodiversity and atmosphere conditions are the foundations for sustainable food security. The ecological damage to these foundations are leading to lowering of productivity and livelihood. This will further aggregate with the threat of climate changes such as temperature, rain falls and sea level rise. As per the exercise by World Food Program, it is indicated that the green revolution states like Punjab and Haryana may become food insecure in another 20 years. Food insecurity is measure in terms of land degradation and salinization, ground water tables and green cover loss. *Based on Key Note address at Global Water Meet for Climate Change Adaptation – Agrarian Perspectives : 24 – 26 OCT. 2016 – UAS – DHARWAD – INDIA
Our Soils
The year 2015 was declared ‘ The International Year of Soil’. This is understandable. A healthy soil is key to farm productivity and healthy life (Annexure – 1). In India, out of 306 m. ha reported under various land uses,

ABSTRACT

Agriculture is an important economic activity in India, contributing around 50% for the employment generation and 14% to the total gross domestic product (GDP) of the nation. Being a subsistence activity, agriculture shares maximum contribution in the national economy. However, the income generated from agriculture is not sufficient to engage the rural youth in farming profession. Lack of economic incentives coupled with other factors like lack of infrastructure for education, and opportunities for employment lead to the migration of the rural youth to the urban areas. Migration of human population can be both interstate as well as intrastate to establish a new permanent or semi-permanent residence. According to the 2001 census, total number of migrants is 314 million by the last residence. Of these 85% of the migration is intrastate while 13% migration is interstate. Approximately 20 million people have migrated from rural areas to urban areas; the reason for migration was predominantly marriage for females, and employment and education for males. This phenomenon has resulted in decrease in the work force in the villages leading over labour population in urban areas – those results in low wages due to competition and wide spread poverty. The situation becomes worse when the youth drop out from the formal education system and move to the urban areas in search of better employment opportunities. This unskilled labour constitute the labour class in the urban areas, and due to the low wages received by them, they remain economically vulnerable. This situation is aggravated by the biased government policies for the rural youth, lack of awareness and ever spreading poverty. Thus, it is important to understand the factors responsible for the youth migration from rural to urban areas and effect of this migration on both rural and urban economy. This paper highlights the issue of youth migration from rural areas to urban areas in search of better education and employment opportunities and its consequences. Further, the policies are described in brief for the involvement of the rural youth in rural economy.

The microbial community is a gold mine for gene(s) and pathway(s) that encode novel biocatalysts for biosynthetic or biodegradation processes, including degradation of pollutants, synthesis of biofuels and production of novel drugs. Sequencing of the microbial genome will also provide insights into the ecology of  microorganisms that are beneficial to, or threaten, crop production, and that ensure the quality and provision of ecosystem services. These unique genetic and biosynthetic capabilities of microorganisms have made them likely  candidates for solving particular difficult problems in the agriculture. For many years microbiologist and microbial  ecologists have tended to differentiate microorganisms as beneficial and harmful according to their function and how they affect soil quality, plant growth, and yield and plant health.

In global competitive agriculture market, the need of the hour is to provide uniform and quality agricultural produce which can compete in market and give remunerative returns. For this, the agriculture production needs to be addressed on more scientific grounds and full package of practices are to be adopted to produce quality produce for global market. Earlier, the orchards were established using seedling material where uniformity of the produce was not guaranteed. Thus, in recent years more emphasis is being paid on scientific establishment of orchards which requires true to type planting material from which uniform produce is expected. This true to type planting material can only be produced by vegetative methods. Thus, standardization of vegetative propagation in horticultural crops, particularly in perennial crops has gained much importance. Some references are available on propagation of plants which shows that vegetative propagation  methods were also practiced in ancient time as mentioned in old treaties literature viz. Upvan vinod,  Ain-e-akbari, etc.