eChapters

Environmental Biotechnology
    
Global Warming and Animal Health and Production
    
Genome Mapping
    
Human Genome
    
Livestock Genomics
    
Cattle Genome
    
Pig Genome
    
Bioinformatics
    
Major Branches of Bioinformatics Include
    
List of Bioinformatics Software Commonly Used in Animal Genomics Research
    
Sequence Analysis Software
    
Genetics Analysis Software
    
Functional Genomics Software
    
Biofuels
    
Advantages of Bioethanol
    
Ecological
    
Economical
    
Looking to the Future: The Potential of Second-Generation Biofuels
    
Future Role of Biofuels Depends on Profitability and New Technologies

Introduction

Healthy seeds and seedlings are the first and essential requirement for achieving full yield potential of any vegetable crop. In recent years, vegetable growers have become highly conscious of the importance of quality seeds or seedlings. A major portion of the area under vegetable cultivation in India is now sown with hybrid seeds, which are costly but give higher yields and quality produce. In view of the high cost of seeds, some vegetable crops like tomato, brinjal, capsicum and cucurbits are being transplanted after growing nursery under protected conditions to achieve maximum germination count and healthy plant establishment. Raising of seedlings in plug trays or portrays is one such technology that achieves the above requirement. This technology is fast emerging as agro enterprise in India for its obvious advantage to both the nursery grower and the farmer. Soil-less substrate consisting of coco-peat and vermiculite is filled in plug trays and one seed is manually placed in each cell of the plug tray. However, manual seeding is labour-intensive operation requiring about more labour and time consuming. Mechanized precision seeding of plug trays is, therefore, necessary to enhance the capacity of rapidly growing nursery industry in India.

Abstract
This chapter describes how modern quantum mechanical methods can be applied in a systematic manner to understand pharmacophore and mechanistic aspects of drugs. This chapter also explains why accurate computation of drug-receptor interactions is important for in silico drug designing and testing.

WHY in silico DESIGNING?
Drug designing is the inventive art of finding a new lead compound based on knowledge of its biological target and then its slow conversion to a compound of therapeutic benefit to patient termed as “drug”. This whole process comprises of long sequels of synthesizing compounds followed by their testing and activity evaluation procedures; often a seemingly endless task until desired potency level is achieved.

Modern Semen Evaluation Methods

1. Fluorescent Microscopic Evaluation
2. In-vitro Fertility Test
3. Enzyme Estimation

1. Fluorescent Microscopic Evaluation

1.1. Acrosomal Integrity (Giemsa method and FITC-PSA assay)

The acrosomal integrity of the spermatozoa was detected using Fluorescence isothiocyanate – Pisum sativum agglutinin (FITC-PSA) staining procedure adopting method of Mendoza et al. (1992). Pisum sativum agglutinin (PSA) when conjugated to Lectin FITC marks the intact acrosome with bright green fluorescence. PSA is an agglutinin from edible pea that binds to glycoconjugates of acrosomal matrix or outer acrosomal membrane. It has affinity for terminal α –D-glucosyl and α-D-mannosyl residues of glycoproteins and it binds specifically to sugar α-mannoside found in acrosomal content.

Introduction

A number of researchers have used morphological descriptors such as growth habit, leaf, shoot, flower, fruit morphology to classify and discriminate different varieties/cultivars and or detect inter and intra-specific variations in different tree species of economic importance (Saran et al. 2006; Holland et al. 2009). Such morphological descriptors are quite homogenous and often found insufficient to distinguish varieties within morphological groups. The choice of suitable cultivars is of paramount importance for its success. The lack of breakthrough has been due to under utilization of genetic variability for superior quality and high yield potential. Most of the fruit tree species demonstrates a rich genetic diversity mainly through natural cross pollination due to self incompatibility (Godara 1980). The elite plant types with desirable traits have been released as varieties, mass multiplied and propagated through standard vegetative multiplication. However, the authenticity of cultivar identification remains unclear (Devanshi et al. 2007).

Evolution is a self-occurring natural phenomenon by which new organisms develop gradually as a result of change in genetic pool of a population. It is a very widely and historically studied phenomenon which still surprises scientists due to its underlying complexity. The three processes which contribute most to evolution are genetic drift (also known as random drift or allelic drift), natural selection and mutations. Genetic drift and natural selection act at a population level, whereas mutations act at individual level. For evolution to occur, the changes these processes bring about must be heritable, which they are.
 
Genes are present on chromosomes inside nucleus of the cell and the collection of all the genes in a cell is known as the genome of that cell. Genome size and complexity varies from species to species. Simple organisms, such as bacteria and protozoans have a very simple genome, with very few genes, whereas complex higher organisms such as humans have a large genome, with thousands of genes. The position of a gene on a chromosome is known as a locus. Organisms may have more than one copy of a chromosome in the cell.

Abstract

The species of liver flukes of the genus Fasciola (Platyhelminthes: Digenea: Fasciolidae)are obligate parasitic trematodes residing in the large biliary ducts of the herbivorous mammalian hosts causing fascioliasis worldwide. While Fasciola hepatica has a cosmopolitan distribution, the other major species, i.e., F. gigantica is reportedly prevalent in the tropical and subtropical regions of Africa and Asia. To determine the phylogenic location of Fasciola sp. of Assam (India) origin based on rDNA molecular data,ribosomal ITS regions were sequenced (Accession numbers- EF027103 and EF027104) and compared with other species of trematodes in the family Fasciolidae that includes, Fasciolopsis buski and Fascioloides magna besides the species of Fasciola. NCBI databases were used for sequence homology analysis using BLAST; multiple sequence alignments were performed using ClustalW program.The phylogenetic trees constructed based upon the ITS (I & II) sequences by multiple tree building methods in MEGA revealed a close relationship with isolates of F. gigantica from China, Indonesia , Japan, Egypt and Zambia, the isolate from China with significant bootstrap values being the closest. Using the novel approach of molecular morphometrics that is based on ITS2 secondary structure homologies, phylogenetic relationships 

Introduction

Since the onset of human civilization molluscs have a tremendous impact on Indian tradition and economy. In India, molluscs have been in great demand as ornaments, currency, as popular panacea for illnesses and as mascot for ward off evil spirits in olden days. Now, molluscs have assumedgreater significance in our industrial, technological and aesthetic aspects of life, in addition to edible utility. Several species of gastropods and bivalves are traditionally fished for food and shell from time immemorial. About 80,000 - 1, 00,000 species of molluscs are recorded from the world over and a total of 3271 species are recorded from India. They are represented in 220 families and 591 general and the spectrum comprise of 1900 gastropods,1100 bivalves,210 cephalopods,41 polyplacophores and 20 scaphopods (edible and ornamental) and 14 species of cephalopods are exploited on commercial basis in India(Appukkuttan et al.,1989). It is difficult to categories shells satisfactorily according to their uses as many are collected for several purposes. The main categories are shown in the Table-1.

Introduction

The demand for agriculture commodity is growing world over and meets the ever increasing targets as per the demand, productivity needs to be raised further. This can be realized in two ways- by increasing area of cultivation and/or through increased efficiency of inputs (both natural and manmade). The scope for the farmer is very limited worldwide and for the latter, the primary input is the solar energy which is most abundant except in few cases like colder regions. In the coming future, the availability of land for cultivation will be a major impendent due to rapid urbanization, hydroelectric projects, dams and rivers, highway roads and also there is a degradation of fertile land due to soil erosion (120.72 million ha), soil salinity and water logging (8.4 million ha) Nath et al., 2007 and Hegde et al. (2015).  Under this situation, multi-storied cropping system will be a viable option to provide food, nutrition and income security to people Awasthi et al., (2008). The available arable land could be effectively utilized for production of field and horticultural crops to achieve nutritional security for the growing population and sustainable income for the farming community. Increasing food production with limited natural resource is a great challenge to the scientific community. Thus, production per unit area of land, time and inputs can be increased by improving efficiency of the rate and extent to which solar energy is harvested for conversion to economic produced.

Abstract

Water Crisis or scarcity can be explained as when demand for fresh water goes high in comparison to the supply of fresh water. The condition is the cause when there is a high rate of aggregate demand from all water using sectors like food, agriculture etc. are compared with available supply under prevailing institutional and infrastructural conditions. It is evident from economic competition from water quantity and quality, disputes between users, irreversible depletion of ground water, and negative impacts on the environment. Water crisis is actually a relative and dynamic concept irrespective of any level of supply and demand. It is a social construct and its causes are directly related to human interference with the water cycle. This scarcity varies over time due to natural hydrological variability. But the stronger reasons could be more of functions of prevailing economic policy, planning and management approaches. 

ABSTRACT

Gene expression profiling holds tremendous promise for dissecting the regulatory mechanisms and transcriptional networks that underlie biological processes. Microarray expression analysis has become one of the most widely used functional genomics tools. Typical approaches to gene identification and functional characterization have and continue to involve protein characterization, peptide sequence determination, and identification of the corresponding DNA sequence. More recently, expressed sequence tags (ESTs), microarrays, large-scale gene expression (transcriptome) profiling, and associated informatics technologies are rapidly becoming commonplace in the sciences. These ‘genomic’ approaches typically take advantage of technologies for characterizing large numbers of nucleic acid sequences, bioinformatics, and the expanding collection of nucleic acid sequence data.

The success of any crop improvement programme springs mainly from sound and genetically investigations carried out in the fields. However, the moment such biological populations are subjected to field evaluation, some external and non-heritable agencies come into active role. As a result, these agencies or factors mask real differences among the tested populations which stem purely from heritable or genetic factors. Their influence gets confounded with the latter. Consequently authentic data related to genetic differences are not available due to controllable and non-controllable errors. Further, physical data that are assembled in an experiment are often so voluminous and so vast that is perhaps humanly not possible to arrive at meaningful conclusions. Hence, an adequate knowledge of statistical principles and their appropriate use warrant perfection to a point where all possible sources of error can be well accounted for.

Climate change has become real and tangible affecting people. It is a major challenge for agriculture, food security and rural livelihoods. Climate change impacts on vulnerable groups have social, economic, political, and environmental implications. The impacts of climate change are global. India is more vulnerable in view of the high population depending on agriculture, which in turn is dependent on the seasonal monsoons. India is confronted with the challenge of sustaining rapid economic growth amidst the increasing global threat of climate change. Government of India has accorded top priority for addressing climate change related concerns.

Nanotechnology is a field of applied science focused on the design, synthesis, characterization and application of materials and devices on the nanoscale. It is a sub classification of technology in colloidal science, biology, physics, chemistry and other scientific fields and involves the study of phenomena and manipulation of material at the nanoscale. This is involved in the creation of useful materials, devices, and systems used to manipulate matter at an incredibly small scale; between 1 and 100 nanometers. With nanotechnology, a large set of materials with distinct properties (optical, electrical, or magnetic) can be fabricated. Nanotechnologically improved products rely on a change in the physical properties when the feature sizes are shrunk.

Abstract

Sundarban is the largest mangrove wetland ecosystems in the world. This World Heritage site along the Bay of Bengal has evolved over the millennia through natural deposition of upstream sediments accompanied by intertidal segregation. Indian part of Sundarbans is estimated to be about 4,110 square kilometres of which about 1,700 square kilometres is occupied by water bodies in the form of river, canals and creeks of width varying from a few metres to several kilometres, which together form the complex ecosystem of this Ganges delta.. Rivers in the Sundarbans are confluence points of salt water and freshwater. Environmental crisis is caused by severe cyclones sea level rise and storm surge followed by coastal erosion, flood and earthquake phenomenon. River water is saline. Shallow aquifer is brackish/ saline, which is not suitable for drinking. Fresh potable ground water is occurring in deeper aquifer, the exploitation of which is not cost-effective. Sundarbans houses around 500 different species of mammals, fish, bird and reptiles and it is the largest habitat of royal Bengal tigers. Conservation of flora and fauna in the typical ecosystem of Sundarbans in India with emphasis on judicious use of ground water resources along with retrospection of historical trend and future sustainable management strategies is a pre-requisite.

1.1. Characteristics and categories
                
1.2. Litter
    Patterns of accumulation
    Successional trends
    Seasonal variations
    Fluctuations in accumulation

1.3. Dissolved organic matter
    Sources of DOM
    Fluxes of DOM

1.4. Woody detritus

1.5. Soil organic matter

Abstract

Never ending additional need of farmers have forced them to grow more cash crop along with crops used for the staple food. The prime crops as per the food habit, social need has to be produced in the respective locality. The micro planning for agricultural and food production has to be planned for different blocks of a district to meet above needs. The production planning has to be simulated first and programmed for trial production. After successful trail, the same has to be replicated in larger area. Mathematical model development approach is one of the solutions to predict the need of the people and plan production depending on the availability of the input materials like natural resources and other inputs. In addition to this food and nutrient security for the people living in those different areas are very important in present context. Global population is growing fast with 1.2% per annum. Feeding these people increases the responsibility of farmers for growing more crops without considering the soil health. Improper crop sequencing has resulted in imbalance in soil nutrient which results in unsustainable production of forth coming crops. 

Nematodes are the most numerous metazoa on earth. They are either free living or parasites of plants and animals. Although currently only about 4100 species of plant parasitic nematodes have been described (i.e. 15 % of the total number of nematode species known), their impact on humans by inflicting heavy losses in agriculture is substantial. Most of the developing countries including India lie in tropical or sub tropical regions where climate is suitable for activity and multiplication of nematodes almost throughout the year. Sandy and warm soils such as found in India other developing countries in the arid zone are very favorable for nematode infection, especially in irrigated areas which are used for crop production continuously. The percentage of crop losses caused by plant pathogens, insect pests and weeds world wide has been estimated to 42% according for $500 billion worth of damage. The estimated overall annual yield loss of world’s major crops due to damage by plant parasitic nematodes has been reported to the extent of 12.3% (Sasser & Freckman, 1987). Estimated losses due to nematodes attack on different cultivated crops all over the world by FAO are around 400 million dollars. Annual crop losses in India is Rs. 242.1 billion. A large number of plant parasitic nematodes have been found associated with pulse crops in allover India but pathogenicity has not been proved for all. Plant parasitic nematodes are one of the most unwanted organisms in agricultural soils. They parasitize the roots of our carefully nurtured crops, reduce their ability to produce yields, make them weak and perhaps vulnerable to many pests and diseases. They reduce the crop yields that may equal those associated with more severe and easily identifiable plant diseases and we do not even realize their presence. Damage to crops by nematodes often goes unnoticed or attributed to other causes such as lack of fertility, deficient soil moisture. Sandy and warm soils such as found in India other developing countries in the arid zone are very favourable for nematode infection, especially in irrigated areas which are used for crop production continuously (Taylor, 1967).

1.    Fruit Crops
    
2.    Spices
    
3.    Masticatory Crops
    
4.    Vegetables
    
5.    Plantation Crops
    
6.    Tuber Crops
    
7.    Medicinal Plants
    
8.    Ornamental Plants
    
9.    Future Prospects
        
References