eChapters

INTRODUCTION

Phalsa, Grewia subinaequalis DC (syn. Grewia asiatica L.) is native to the Indian sub-continent and South-East Asia and belongs to family Tiliaceae, to which jute, our major money earning fiber crop, also belongs.Phalsa has been mentioned in our Vedic books and is native to India and other parts of southeast Asia, including Pakistan, Sri Lanka and Bangladesh. It is known by many names viz., Hindi (Phalsa, Shukri, Tadachi, Dhaman, Parusha); English (Indian phalsa, phalsa); Bengali (Phalsa, Shunkri); Kannada(Phulsa), Telegu (Phutiki), Tamil (Unnu), Malayalam (Chadicha) Gujarati (Shukri), Filipino (Bariuangulod) and Thai (Potaohai,Yapkheethao,Malai,Laikhon). The plant is commonly known as Gangeran. Grewia species is one of the best examples of multipurpose fruit species which are useful source of food, fodder, fiber, fuelwood, timber and a range of traditional medicines which cure a number of diseases (Sharma and Patni, 2012). 

A. Bhattacharya
    
5.1.    Chlorophyll
    
5.2.    Response to Drought Stress
    
5.3.    CO2 Fixation Under Drought
    
5.4.    Response to High Temperature Stress
    
5.5.    Response to Low Temperature Stress
    
5.6.    Effect of Cold Stress on CO2 Fixation
    
5.7.    Cold Acclimation and its Role in Providing Freeze-tolerance
    
5.8.    Response to Salinity Stress
    
5.9.    CO2 Fixation Under Salinity Stress
    
5.10.    Response to High Light Intensity Stress
    
5.11.    Stress-induced Changes
         
    5.11.1.    Effects on Photosynthetic Pigments
    5.11.2.    Effects on Photosystems
    5.11.3.    Effects on Gas-exchange Characteristics        
    5.11.4.    Effects on Activities of key Photosynthetic Enzymes
    
5.12.    Improvement in Photosynthetic Capacity Under Stress by Engineering Photosynthesis-related Genes or Transcription Factors
    
    5.12.1.    Transfer of C4 genes/traits to C3 plants
    5.12.2.    Role of Transcription Factors in Regulation of Genes Involved in Photosynthesis
    
5.13    Role of Mitogen-activated Protein Kinases (MAPKs) in Photosynthesis
    
5.14    Conclusion and Future Prospects
        
References

Introduction

Microbes (bacteria, archea and eukarya) are single celled organisms that rule the planet. Microbes occupy every possible niche, from the high atmosphere to the deep ocean, from hot springs to artic ice, from hot deserts to deep forests including many regions that cannot yet be accessed for study. The biosphere contains between 1030 and 1031 microbial genomes, which is about 2-3 times more than the number of plant and animal cells combined (67). In fact, studies have shown that microbes associated with the human body are about ten times more than the number of human cells (5).  Microbes play a critical role in the life cycle of all living organism and environment in general.  Some microbes cause disease, but the most of them are either innocuous or play a role in physiology and cellular response including immune response, digestion and vitamin production of various organisms.

The Fourth Assessment Report of the United Nations Intergovernmental Panel on Climate Change (IPCC), after analysis of more than 6000 peer reviewed publications has assessed potential effects of changing climate on various spheres of life (Solomon et al., 2007). Under different scenarios, the estimated temperature rise of 1.1 to 6.4 °C in the global surface temperature is predicted at the end of 21st century (period 2090-2099 relative to 1980-1999). Global average sea level in the last interglacial period (about 125,000 years ago) was likely 4 to 6 m higher than during the 20th century, mainly due to the melting of ice in the polar regions. It has been detected in changes of surface and atmospheric temperatures in the upper several hundred metres of the ocean, and in contributions to sea level rise (Solomon et al., 2007). The change in climate is concurrent with the changes in global levels of carbondioxide, methane, oxides of nitrogen etc, loss of biodiversity, natural habitat and other natural resources. The IPCC report also estimates a confidence level of 90% that there will be more frequent warm spells, heat waves and heavy rainfall and a confidence level of 66% that there will be an increase in drought, tropical cyclones and extreme high tides. The magnitude of the events will vary depending on the geographic zones of the World (Solomon et al., 2007).

• In male animals, endocrine regulation of reproduction is mainly controlled by pituitary gonadotrophins and gonadal androgens. It is not same as in females as there is only negative feedback controls in case of male while it is positively as well as negatively feedback control in females. In other words the synchrony of pituitary gonadotrophins with gonads in male is non cyclic while in female it is cyclic i.e. after castration the pulse frequency of LH and FSH is retained
• Sex Hormones are sex specific e.g. estrogen and progesterone works primarily in females and testosterone in males but sex specificity is limited as far as sexual behaviour is concern. As estrogen injection in castrated buck recovers the maleness while testosterone injection in ovariectomized female maintain the receptivity for male up to certain extent.
• Hypothalamus acts as interface between nervous system and endocrine system. GnRH synthesized in neurons in hypothalamus, released at nerve endings episodic manner and transferred via hypophyseal portal system to anterior pituitary to stimulate the secretion of gonadotrophins.

INTRODUCTION

Plant growth regulators (PGR) are bioactive molecules that modulates the physiological processes in plants and are effective at ultra-low concentrations. Minute alteration in the concentration and bioactive forms of PGRs’ throughout the plant development phases regulate the flowering, fruit set and development. The use of plant growth substances to control fruit set, size, shape, and maturation has become important in agriculture today because they have the ability to increase fruit size, colour, and shape, thereby increasing the marketability. By hastening or delaying maturation the grower can utilize peak demands, avoid unfavourable environmental conditions, and extend the market period. PGRs are applied in fruit crops at all developmental stages for various purposes as discussed below: 

INTRODUCTION
 
Environment and ecological set up is maintained by the climatic conditions, presence and compositions of gases present in them, humidity level, temperature and the pollution. These conditions are responsible for the emerging of diseases in the environment. The diseases in plants, animals and human beings are due to the factors that affect the ecology. This also depends on the organism whether they are susceptible or resistant to the specific disease. This means the genetic level of the organism is an important factor.
Plant pathology or phytopathology is the study of diseases in plants. The disease evolution may be due to the climatic condition, humidity level, temperature and the genetic character of the plant and the pathogen. The parasitic adaptation of microorganisms to be plant pathogens includes acquisition of the following three abilities: 1) ability to enter into a plant, (2) ability to overcome the resistance of the host and (3) ability to evoke disease.

INTRODUCTION

Pineapple [(Ananas comosus (L.) Merr., Bromeliaceae)] is a commercial, tropical American crop, popularly known as “Golden Queen” all over the globe. The name ‘pineapple’ was given in the mid-17th century because of its resemblance to ‘pine cone’. Its botanical name Ananas comes from ‘anana’, the Tupi word for the fruit, meaning “excellent fruit” and comosus means tufted and refers to the stem of the fruit.
 
Pineapple is a monocotyledonous, low-growing,herbaceous and perennial with a spiral of spiny sword-shaped leaves on a thick stem. It has 90 to 100 cm height with spreading leaves, which give the plant a rosette appearance.The plant bears a single fruit terminally on a peduncle protruding out from the centre of the rosette. The fruit is mostly a large juicy fruit containing aromatic, edible, yellow flesh surrounded by a tough segmented skin and topped with a tuft of stiff leaves.

A. PLANNING OF AN ORCHARD

Orchard is a long-term investment and needs proper planning and layout before establishment. Mistakes committed during planning and planting of fruit trees cannot be rectified. While planning and planting a new orchard, it is very important to carefully assess all the factors that will ultimately affect fruit yield and orchard sustainability. The aspects like location and site selection, suitability of soil and subsoil, suitability of fruit crop and varieties, plant spacing, market potential and source of nursery plants must be taken care of seriously. Thus, one should avail of expert advice on the establishment of new orchards and their proper maintenance.

Abstract

Plant architecture, plays a crucial role in gathering multiple resources from the environment. As a plant grows, they change its architecture with the surrounding environment for altering available resources. The architecture of the plant is modified through the distribution of assimilate, which conclude the growth rates of different organs. Plant roots expand to acquire soil water and nutrients (e.g. nitrogen, phosphorus) from different soil layers by adjusting their direction in order to growth which transported and allocated to whole plant for maintenance and expansion of plant organs. All plants gradually develop their architecture according to source–sink activities and endogenous signals. This adaptation can result in complex structures. This chapter deals with the importance, different method for changing the architecture   of vegetable crops for improving their yield and quality.

A. General
        
B. Leaves and Floral Terminologies
        
C. Diversity in the Flower
        
D. Diversity in Fruits
        
E. Terminologies Related to Seed

Abstract

DNA barcoding, an additional global standard taxonomic tool for identifying plant species helps the non-taxonomists also to identify the plant species in a more reliable way with the help of devices including nanotechnology. Researchers proposed various genes or gene portions as potential barcodes either singly or in combination, yet there is no standard/ universal barcode DNA region for plants. The more accurate plant DNA barcode region would have to be from highly variable markers in the nuclear genome preferably from multiple loci and in combination with variable DNA regions of chloroplast genome, to resolve the problems that arise due to hybridization, allopolyploidization and introgression. Nonetheless, the phylogenetically informative sites might not be the potential DNA barcode regions as the former requires synapomorphies unlike autapomorphies with high levels of informative characters in the latter DNA taxonomy, but could provide insights into genetic distances between species.

1.     Isolation of Plant DNA
    
2.     RAPD Analysis of Genomic DNA
    
3.    Agro Bacterium based Transformation in Plants
    
4.     Assay for Gus Operon
    
5.     Confirmation of Transformation using Polymerized Chain Reaction (PCR)
    
6.     Primer Designing
    
7.     Isolation, Purification and Identification of Plant Secondary Metabolites
    
8.     Evaluation of Antioxidant Activity of Plant Extract
    
9.     Estimation of Total Phenolic Content in A Plant Extract
    
10.     Estimation of Total Flavanoid Content

SYSTEMS BIOLOGY

The ultimate goal of any biological experiment is to understand biological systems in sufficient detail to enable accurate, quantitative predictions about the behavior of the systems, including predictions of the effects of modifications of the systems. To precisely simulate the behavior of the system using a computer model is one of the ways to achieve the above goal and in this venture we are nowhere close to the goal. Systems biology will help us to get closer to this goal and it is expected that biologists to adopt to take advantage of the power of systems biology approach. Systems biology is an emergent field that aims at system-level understanding of biological systems. Systems Biology is the study of the interactions between the components of biological systems, and how these interactions give rise to the function and behavior of that system. For example, theenzymes and metabolites in a metabolic pathway.With the progress of genome sequence projects and range of other molecular biology projects that accumulate in-depth knowledge of molecular nature of biological system, we are now at the stage to seriously look into possibility of system-level understanding of solidly grounded on molecular-level understanding.Unlike molecular biology which focus on molecules, such as sequence of nucleic acids and proteins, systems biology focus on systems that are composed of molecular components. Although systems are composed of matters, the essence of system lies in dynamics and it cannot be described merely by enumerating components of the system.

1.     Plant Tissue Culture Laboratory
    
2.     Cleaning and Sterilization of Glass Wares
    
3.     Media Sterilization
    
4.     Media Composition and Media Preparation
    
5.     Callus Culture in Carrot
    
6.     Culture of Shoot Tip (Vanilla)
    
7.     Anther Culture of Datura
    
8.     Subculture of Carrot Callus
    
9.     Hardening of Plantlets
    
10.     Isolation of Protoplast from Plant Leaves

1.    Land Preparation
    
2.    Tillages
    
3.    Planting
    
4.    Field Maintenance
    
5.    Vegetation Control
    
6.    Animal/ Bird Control
    
7.    Insects and Diseases Management
    
8.    Irrigation
    
9.    Manuring
    
10.    Harvesting
    
11.    Collection and Transportation
    
12.    Integrated Mechanization
        
References

INTRODUCTION

The European plum (Prunus domestica L.) is the most important plum species grown in different countries in the world. The Japanese plum (Prunus salicina Lindl.) is the next important species grown in many Asian countries. It is more vigorous, productive, precocious in bearing and more resistant to diseases than the European plum. The Japanese plum a temperate fruit is unique amongst the stone fruits. It is a strong small growing tree, performing well as major crop of hills and considered as important minor fruit crop in north India. Its low chilling varieties comes into the market when the other crops are scarce and find little competition in the market. The fruit growers are now preferred to grow Japanese plum on account of giving higher yield and excellent return per unit area. It is successfully grown in temperate zone and subtropical climate of northern Indian states. Moreover, it is preferred as filler tree in permanent orchards of mango, litchi and pear. It start bearing after 2-3 years of planting and giving good economic return to the growers during pre-bearing period of permanent fruit crops.

2.1. INTRODUCTION

Pollination is the process of mating in plants, that is, the transfer of pollen from anther to stigma. In flowers, pollen delivered to the stigma through a wide range of mechanisms that ensure an appropriate balance in the genetic makeup of the species. Most plant species have evolved ways to ensure an appropriate degree of interchange of genetic material between individuals in the population, and cross-pollination is the normal type of pollination. Plants benefit most by being pollinated by other individuals because this broadens the genetic characteristics of individual plants. As a result, they are more adaptable to necessary changes. Self- and often-pollinated crops evolved from cross pollinated crops.

Pollination followed by fertilization is important in agriculture, particularly for the crops whose economic part is either fruit or seed, such as, rice, wheat, fruit vegetables etc. For other crops, these events are also important for production of seed for cultivation of next generation, for examples, green vegetables (amaranth, palak, cabbage, cauliflower etc).

The study of pollination brings together many disciplines, such as botany, plant breeding, horticulture, entomology, and ecology. The pollination process as an interaction between flower and vector was first addressed in the 18th century by Christian Konrad Sprengel. It is important in horticulture and agriculture, because fruiting is dependent on fertilization, which is the end result of pollination.

1.     Groups of Polyembryony (PE)
    
2.     Mechanism of Polyembryony and the Genetic Bases
    
3.    Classification of Polyembryony
    
4.    Polyembryony – Its Practical Implications
        
References

INTRODUCTION

The pomegranate(Punica granatum L.) is one of the oldest known edible fruits, occupies the eighteenth place among the main world-fruit cultures.The fruit was naturalized throughout the Mediterranean region through ancient times and today the main areas of world production are Turkey, Spain and California. Around the world pomegranates are generally grown in Mediterranean climates, often with very warm dry summers (Wetzstein et al., 2011). During recent past, this ancient fruit has emerged as commercially important fruit, owing to its enormous medicinal and nutritional properties, built-in ability to tolerate heat and drought, low resource input demanding nature and high returns on investment (Singh et al.,2012). The fruit has growing consumer demand both for fresh use as well as processing into juice, syrup, squash and anardana (an acidulant product), owing to its attractive, juicy, and refreshing arils (Pruthi and Saxena, 1984).