eChapters

Basic principles of breeding hybrid maize
1. Inbreeding
•    Produce a plant which breeds true
•    Produce uniform plants
•    Increase the homozygosity at all or specific loci in the plant genome
•    We know what inbreeding does and I do not propose to spend people’s money to learn how to reduce corn yield-Hopkins

MUTATION BREEDING

Mutation refers to sudden heritable change in phenotype of an organism or permanent change in the number, kind and sequence of nucleotides in genetic material, while individuals showing these changes are known as mutants. Mutation was first discovered by Wright in 1791 in male lamb which had short legs. Hugo de vries coined the term mutation. In crop improvement the breeders are always look for wide range of variability and stabilization of desirable traits. Mutation breeding is very useful in inducing new variability, which is an essential requirement of any plant breeding programme. The modern day cultivars are the resultants of various breeding techniques like selection, hybridization, tissue culture and mutagenesis including some biotechnological tools. Novelty in commercial traits like colour, size, shape, length, post harvest life of flowers etc. are always valued and preferred by consumers. Mutation breeding by exposing planting material with irradiation has play vital role in inducing novelty in a number of flower crops.

Feeding of green and succulent fodder is of utmost importance to farm animals. Grazing fresh forage year round at the optimal stage of maturity would supply the highest quality and most palatable form of feed in any livestock operation. In most part of the world, green fodder is limited to a particular season only. Because of fluctuations in seasonal growth and plant maturity, excessive biomass in the spring, it is necessary to harvest and store forages to maximize both quality and productivity. One of the important form of conservation practiced on a wide scale is hay making. Hay making is an art in which the fodder is kept for future use in lean period. Since it contains large quantity of moisture it is to be dried properly to increase its keeping quality without reducing its nutritive value. The objective of hay making is to achieve a rapid moisture loss after cutting so that the forage can be romoved from the field with minimum losses from weathering and microbial degradation (Bareebam, 1992).

Arterial diseases are important because of their serious effect on vital organs like brain, heart and kidney. Thrombosis is the most common complication of arterial diseases but hemorrhage and aneurysm formation may occur.

Carbohydrate metabolism in the cell is essentially the metabolism of glucose and other substances related to glucose in their metabolic processes. The chemical reactions involved in carbohydrate metabolism represents complex groups, sequences, and cycles of reactions which integrate at various points with the reactions concerned in the metabolism of lipids and proteins. The major types of chemical processes involved in carbohydrate metabolism may be grouped as follows: a) Anabolism of starch – Photosynthesis, b) Catabolism of starch, c) Catabolism of glucose – 1. Glycolysis and TCA cycle and 2. Oxidative pentose   phosphate pathway.
 
Plant cells “burn “ glucose for energy. Plants also produce sugars through photosynthesis. This is roughly the reverse of the burning process.

Introduction

India is the largest developing economy of the world and about 70 per cent of its population is dependent directly or indirectly on this profession.  To meet the food requirement of the ever-multiplying population of this country, agriculture production has to be maximized by using genetically improved varieties, fertilizers improved implements, seed, and other agronomical technologies. Beside these improvements the agriculture production has remained static for the last decade. For further improvements in agricultural production we have to adopt comprehensive strategies. One of such strategies is the pollination management that is a very vital process of agriculture crop production. Pollination is a process involving reproductive parts of a flower and an external agent in the form of a vector. According to Prof. E.C. Martin, the value of bee pollination of fruits, vegetables and seeds in the United States of America totaled very close to $8 billion in the 1960s; the figure 20 years later is probably close to $20 billion.

Curry leaf (Murraya koenigii Spreng. Syn. Bergera koenigii Koen (N.O Aurantiaceae), Chaleas koenigii Curz ex. Swingle) is a perennial leaf vegetable. It belongs to family Rutaceae. Its genus name ‘Murraya’commemorates John Adam Murray, Professor of Botany at Gottingen and editor of many of Linnaeus’s works. (Fyson, 1977).

Origin and Distribution

The crop originated in the tarai tract of Uttar Pradesh (India), in the foothills of the Himalayas. Wild forms with less pungent leaves are seen along the plains and hills of Himalayas extending from Kumaon to Sikkim, in Bengal, Assam, Deccan plateau and in Western Ghats. At present, it is cultivated in Myanmar, SriLanka, China, Australia and Pacific Islands. The curry leaf grows throughout India including the Andaman Islands up to an altitude of 1500 m amol (Wealth of India, 1962). Dastur (1970) recorded its wild form in Garhwal hills in Uttaranchal, Sikkim, Bengal, Assam, Central India, Western Ghats, Tamil Nadu and Kerala.  Used as a flavourant and as a leaf vegetable by the early inhabitants, Dravidians, curry leaf was introduced to Southern India when the Dravidians moved southward after the arrival of Aryans from Persia. It is a backyard crop in many of the South Indian homesteads.  The spicy leaves of plants are used extensively for seasoning and flavouring dishes.

The method described here gives an approximate thioglucoside and isothiocyanates contents in rapeseed meal.

Reagents and Apparatus
    1.    Barium chloride (5% solution)
    2.    Volumetric flask, 600 ml
    3.    Steam bath

Method

To 10 g meal (defatted by soxhlet extraction) add 250 ml distilled water, hydrolyse at 54ºC for one hour and then boil for two hours, keeping volume constant. Filter, retaining filtrate and wash residue three times with 50 ml hot water. Add washings to initial filtrate and make up volume to 600 ml. Precipitate barium sulphate by heating and adding excess barium chloride solution. Leave on a steam bath for a few hours and then filter. Ash in muffle furnace and then weigh precipitate.

Q.1.    What are the major clinical findings in mastitis?
    Ans.    These are abnormalities of secretions, abnormalities of udder related to size, consistency, temperature and sometimes systemic reactions.

Q.2.    How clinical forms of mastitis could be classified based of severity and duration?
    Ans.    Based on severity it is classified in four forms: Per acute mastitis, acute mastitis, subacute mastitis and subclinical mastitis. However, based on duration it is of three forms: Short term clinical or subclinical mastitis (Coliform mastitis), recurrent mastitis (Staphylococcus aureus mastitis, Streptococcal dysgalactiae mastitis) and persistent clinical or subclinical mastitis (Streptococcus agalactiae mastitis).

Q.3.    What is sub clinical mastitis?
    Ans.    In sub clinical mastitis there is evidence of inflammation which is evidenced by only raised SCC in milk without any visible abnormalities of milk and udder.

Tuberose (Polianthes tuberosa L.) belonging to family Amaryllidaceae is half hardy, perennial, bulbous plant. Bulbs are made of scales and leaf bases and stem remains concealed within scales. The generic name Polianthes is derived from Greek word “polios” meaning shining or white and “anthos” meaning flower, in allusion to the blooms of the common tuberose and species tuberosa, the plant being tuberous in nature. Tuberose is commercially cultivated in tropical and sub tropical areas for cut and loose flower trade and also for the extraction of it’s highly valued natural flower oil.

ORIGIN AND DISTRIBUTION

Tuberose is a native of Mexico (Bailey, 1976) from where it spread to different parts of world during 16^th century. The name tuberose is derived from tuberosa, this plant being the tuberous hyacinth as distinguished from the bulbous hyacinth. The name therefore, is tuber-ose, not tuberose. By the middle of the 18^th century the tuberose was still referred for as Hyacinthus indicus tuberosus. Originally Linnaeus had used the name Polianthes floribus alternis in his Hortus Cliffortianus. Later it was grouped under the genus Polianthes and the species tuberosa.

Tuberose is cultivated on large scale in China, Egypt, France, Italy, Israel, South Africa, New Zealand, Spain, Sri Lanka, North Carolina, USA and in many tropical and subtropical countries.

In India tuberose is commonly known as Gulchari and Gulshabbo in Hindi, Rajanigandha in Bengali, Sukandaraji and Nelasampengi in Telegu, Nilasampangi in Tamil and as Sugandharaja in Kannada.

In India its commercial cultivations is confined to Bagnen, Midnapur, Krishnanagar, Ranaghat, Kolaghat and Pariskura in West Bengal, Guwahati and Jorhat in Assam, Pune, Thane, Sangli, Nasik and Ahmednagar in Maharastra, Udaipur, Ajmer and Jaipur in Rajasthan, Navsari and Valsad in Gujrat, Ambala, Gurgaon and Faridabad in Haryana, Ludhiana and Patiala in Punjab, Devanahalli, Tumkur, Kolar, Belgaum and Mysore in Karnataka, East Godavari, Guntur, Chitoor and Krishna district in Andhra Pradesh and Coimbatore and Madurai in Tamil Nadu. Growers of Uttar Pradesh, Delhi and Orissa are also concentrating in tuberose cultivation after evolvement of improved varieties namely Shringar, Suvasini, Prajwal and Vaibhav by Indian breeders (Singh, 2006).

Kalamata / calamata: It is brine cured black olive grown in Greece.

Kamucha: Tea cider or fermented tea beverages is known as kamucha.The fermentation process take place by the presence of yeast and bacteria

Kanji: Clean, black coloured, crisp and sound product of characteristic flavor prepared by full fermentation (chiefly lactic acid fermentation) of black carrot with 2.5% salt, 1% powder mustard and some amount of water. It is fermented to make ready to serve drink which is known as Kanji. Each kilogram of the grated carrots yield just 7-8 litres of kanji.

Karl Fischer Titration: Karl Fischer Titration is used for estimation of moisture content of foods (low moisture products).

• Agriculture Database Concepts and Importance
• The Orientation of Topic Selection for the Construction of the Agricultural Database
• The Principle of Specialization in the Construction of the Agricultural Database
• Top Quality Principle of Building Databases
• Agriculture Database Types
• Agriculture Libraries
• Agriculture Journals
• Technical Reports
• Conference Papers
• E-Books in Agriculture

Q.1.    What is an experiment ?

    Answer
    
    An experiment is a means of getting an answer to the question that an experimentor (researcher) has in mind. This may be to decide which of the several pain relieving drugs that are available in the market, is the most effective or whether they are equqlly effective.
    
    An experiment may be planned to compare the yield of different varieties of wheat. so that the varity giving the maximum yield may be recommended to the farmers to increase their production.

Introduction

The literatures and researcher data showed that there is negative impacts of agricultural expansion, landscape modification and deforestation on biodiversity, ecosystem services, alteringthe species composition and their ecological functions (Tscharntke et al. 2005; Priess et al. 2007; Flynn et al. 2009; Senior et al. 2013; Deguines et al. 2014; Kumar 2016) which can lead to considerable changes in critical ecosystem processes. On the other hand, an increasingly industrialized global economy, rapid population growth, land degradation, land use pattern and role of various human activities have led to dramatically increased the pressure on the natural resources such as the available land for sustaining the livelihoods, and with over exploitation and extraction of the natural resources the ecosystems are becoming unsustainable and fragile since last century (Kumar 2017). Standardization of cultural practices is one of the primary objectives to make the system ecologically, sustainable and economically viable (Gill et al.2009).

Introduction

The hills and mountains are one of the most important agro-ecosystems that supports life of half of the mankind either directly or indirectly. In India, hills are rich source of bio-diversity and possess enormous potential for sustaining agriculture, including horticulture and animal husbandry. The ecological fragility and vulnerability of the Himalayas to climatic aberrations, increasing demand for land to grow more food have been issues of concern for quite some time. Nearly 59% of the total work force in the Himalayas is engaged in subsistence agriculture which is barely enough to feed the growing population for 5-6 months in a year (Raizada et al., 2009). The North-West Himalayas (NWH) consisting of Jammu & Kashmir, Himachal Pradesh and Uttarakhand spreads to 33 million ha, habited by 25 million humans and 19 million livestock. Though, the whole economy of the region is based on agriculture, the total cultivable land is only 3.2 million ha of the total geographical area of the region (Srivastva et al., 2009).

Chromosomes are giant molecules of DNA

Found in nucleus: never leave except in cell division

Carry hereditary units, genes

Two homologous copies in diplod cells

Unwind when replicating or making RNA

Wind up (condense) in cell division

Attach to spindle and separate in meiosis & mitosis

Crossing over in prophase of 1st meiotic division

Chromosomes are equally distributed in mitosis, meiosis

Gametes have half the number of chromosomes as body cells

Sister chromatids are observed to split apart longitudinally in anaphase

Isolation of plasmid DNA from E. coli is a common routine in research laboratories. One can perform a widely-practiced procedure that involves alkaline lysis of cells. This protocol often referred to as a plasmid “mini-prep,” yields fairly clean DNA quickly and easily.

Procedure

Fill a microcentrifuge tube with saturated bacterial culture grown in LB broth + antibiotic. Spin tube in microcentrifuge for 1 minute, and make sure tubes are balanced in microcentrifuge. Dump supernatant and drain tube briefly on paper towel.

Repeat step 1 in the same tube, filling the tube again with more bacterial culture. The purpose of this step is to increase the starting volume of cells so that more plasmid DNA can be isolated per prep. Spin tube in microcentrifuge for 1 minute. Pour off supernatant and drain tube on paper towel.

Add 0.2 ml ice-cold Solution 1 to cell pellet and resuspend cells as much as possible using disposable transfer pipet.

12.1 Introduction

Man is the most beautiful of all God’s creations and like man, his environment too is beautiful with rivers to quench his thirst with sweet water, atmosphere to provide him oxygen, land and fire to provide him food. Primitive man ate uncooked food and lived in caves. Soon he learnt the use of firewood for cooking of raw meat and provided the first instance of ‘Pollution from food processing’ in history. As man shifted from one place to another, this sort of living never polluted the environment. But as the man ascended the ladder of civiliz ation, his needs grew in arithmeti c progression but corresponding pollution grew in geometric progression. With increase in agriculture produce, a need was felt to preserve the glut production in the form of processed foods giving rise to massive development of food processing industries all over the world. The rapid industrialization of agriculture and horticulture, increased the pollution load many folds especially the biodegradable waste from food industry [41].The wineries and distilleries (distill wine to make brandy) are no exception. Nevertheless, the waste is a precious source of several useful materials as such or after fermentation [1,31,46,86]. Thus, development and environment are two sides of the same coin.

From time immemorial, the biosphere is acting as a sink for all the waste products and recycles them to make good the loss so that every generation finds it the same as one before it. But, of late, this self-cleaning and equilibrium maintenance of biosphere is disastrously disturbed as the waste produced from man’s activities is continuously exceeding the capacity to purify herself. Consequently, our biosphere is becoming more and more poisonous and days are not far away when this planet will become inhabitable.