eChapters

Requirements: Clipboard, Pencils, Hand lens, Pocket knife, insect catching nets, attractants, spread sheet Collection bags and vials, Camera etc.

Sampling techniques are used to draw valid inferences about the population parameters using the sample statistics

Components of an insect sampling program 

Knowledge of pest and beneficial insects

Identification

 Life cycle and biology

Injury caused

Sample: Sample is a subgroup of the population. It is defined as a collection of individual observations from the population about which inferences are to be made, and is obtained by a specific method.

Scale insects:Pseudaulacaspis cockerelli, Parasaisettia nigra:Coccidae

Attack Adults and nymphs feed on plant sap and cause wilting and drying of seedlings and young plants.
 
Brown soft scale: Lecanium sp. (=Coccus) hesperidum : Coccidae

Principle

The residue remaining from the hemicellulose determination is digested with 72 percent sulphuric acid at room temperature. The lignin and acid insoluble  ash are retained in the filter crucible while the digested carbohydrate passes through. The lignin is separated from acid insoluble ash by ashing the residue.

Equipment

    1.    Acid digestion apparatus. A glass tray about 18 cm x 30 cm, 4 cm deep will hold twelve, 100 ml Griffen beakers. Another tray of the same size can be inverted over the beaker.
    2.    Filter crucibles. Same as described for cell wall determination.
    3.    Filtering apparatus. Same as described for cell wall determination.

• Introduction
• The Concept of a Farm Management System

Principle

Pesticide residues are extracted from soil using a mixture of acetone/water in a soxhlet apparatus for 7-8 hours. This extract can be determined directly by GLC.

Scope: This method is suitable for most OPs, OCs, SPs, and herbicides residue analysis.

Biological activity or index of a soil is the function of number of organisms present in soil and their physiological efficiency. The rate of respiration can be used as an index of the biological activity of soil as it reflects the physiological efficiency of the organisms. Monitoring of dehydrogenase, which are respiratory enzymes and integral part of all soil organisms, will give a measure of biological activity of soil at a given time.

During respiration biological oxidation of reduced compounds occur which is catalyzed by dehydrogenase. In the process TTC gets reduced to a pink coloured compound Triphenyl formazan (TPF) which can be quantitatively extracted by methanol and measured colorimeterically. The soil dehydrogenase activity in the current experiment was measured as per the methods of Cassida, (1977).

Observation recording: Phenological events are typically quantified by observing the date of onset and the duration of particular phenophases, which include both vegetative and reproductive events like leaf shedding, leaf emergence, flowering and fruiting. To record the phenological events students should note down the emergence of leaf, flowering and fruiting at the interval of 15 days by vising the targeted tree species.

It is more common in old dogs and also mostly observed in the cutaneous region of trunk and legs.

Grossly, the tumour appears as solitary to multiple masses as white, pink to red, smooth, and raised nodules. Alopecia and ulceration are also observed. It is mostly observed in the pinna and digits.

Thrips: Thrips tabaci: Thripidae

The nymphs and adults of the thrips desap from the narrow spaces between the inner leaf axils. Leaves are faded, with curled tips and shining silver streaks or brown spots.  Many minute yellow or white insects are seen moving, generally near the base or middle of leaves and they suck the sap from the plants.  Plants are faded and the yield is reduced.

Principle

The residue from the cell walls determination is extracted with boiling 8 percent sulphuric acid for one hour to hydrolyse the hemicellulose without dissolving cellulose, lignin or acid insoluble ash.

Equipment
    1.    Refluxing apparatus.
    2.     Filtering apparatus: same as described for cell wall determination
    3.    Filtering crucibles: same as described for cell wall determination.

Reagents

    1.    Sulphuric acid (8%) : Standardize Reagent grade sulphuric acid to specific gravity of 1.052 at 20ºC.
    2.    Bromocresol green indicator solution: Same as used for lignin determination.

Indication

Irreparable injury.

Frost bite, burn.

Malignant neoplasia.

Incurable peripheral vascular disease.

Necrosis, gangrene, extensive injury of nerve of fore limb.

Principle

Pesticide residues in milk are derived from the intake of pesticide contaminated feed and fodder.

Scope

This method is suitable for most OPs, OCs, SPs, and herbicides residue analysis.

Fluorescein diacetate (3’ 6’-diacetyl-fluorescein) is a fluorescein conjugated to two acetate radicals. This colourless compound is hydrolysed by both free (exoenzymes) and membrane bound enzymes, releasing a coloured end product, fluorescein. This end product absorbs strongly in the visible wavelength (490 nm) and can be measured by spectrophotometry. The enzymes responsible for FDA hydrolysis are plentiful in the soil environment. Non-specific esterases, proteases and lipases, have been shown to hydrolyse FDA. The hydrolysis of FDA by soil enzymes can be estimated by methods of Adam and Duncan (2001).

Objectives

1. To know the propagation and its methods.
2. To know the propagation methods of various spices and condiments.

Propagation of plants

The process of increasing plant numbers is called propagation

Or

Multiplication of plants is known as propagation

Plants are propagated either by

1. Sexual (Seed) or
2. Asexual (vegetative) methods.

Observation recording: Phenological events are typically quantified by observing the date of onset and the duration of particular phenophases, which include both vegetative and reproductive events like leaf shedding, leaf emergence, flowering and fruiting. To record the phenological events students should note down the emergence of leaf, flowering and fruiting at the interval of 15 days by vising the targeted tree species.

It is more common in dogs and frequently seen in the skin and oral cavity. It usually occurs in 5 to 11 years old dogs. Benign melanomas are solitary, circumscribed, alopecic firm and freely movable blue black coloured nodules in the skin. Malignant melanoma is sessile, polypoid to plaque like grey or brown black coloured granules in the skin with ulceration.

Indication: Irreparable damage of the meta tarsal joint.