eChapters

14.1 Introduction

In this chapter we shall describe various simple and easy to fabricate fiber optic sensors. Most of these sensors are based on intensity modulation. Intensity modulated optical fiber sensors have been extensively studied in comparison to sensors based on other modulation techniques. This is because of their several advantages such as simplicity, reliability, flexibility and relatively low cost. Intensity modulated sensors make use of multimode fibers. Multimode fibers have large core and high numerical aperture that make the coupling of light into the fiber easier. The large core is also better suited to propagate high power laser beams. Further, the plastic-clad fused silica multimode fibers are available. Plastic-clad silica multimode fibers have an additional advantage. Their plastic cladding can be removed easily permitting access to the core. The evanescent field in the unclad part has been used for various kinds of chemical and biochemical sensors (see chapter 5). Above all, these fibers are cheap. This chapter is devoted to simple and easy to make both intrinsic and extrinsic type sensors. Sensors utilizing bare-ended fibers have also been described.

The following tests are discussed under this section:
    16.1    Estimation of ascorbic acid concentration
    16.2    Effect of cold shock
    16.3    Mitochondrial activity
    16.4    Capacitation of spermatozoa and calcium influx 
    16.5    Morphometric analysis
    16.6    Flow cytometry

16.1 Estimation of Ascorbic Acid Concentration

Ascorbic acid concentration in semen is beneficial for its antioxidant action. It protects the spermatozoa against oxidative damage of free radical produced in the semen.

Materials Required

    1.    Test tubes with stand
    2.    Burrette with stand
    3.    Centrifuge tube with stand

13.1 Introduction
In the previous chapters, we have considered different tests of significance based on normal distribution, F distribution, student’s t distribution and Chi-square (χ2) distribution which are frequently applied by the users of different disciplines of applied sciences and social sciences. In this chapter some more tests of significance are considered which have not been covered in those chapters. These tests are also based on t, F, Fisher’s z transformation, χ2 and some other test statistics are also discussed one by one in the following sections.

13.1 Introduction

In the previous chapters, we have considered different tests of significance based on normal distribution, F distribution, student’s t distribution and Chi-square (x²) distribution which are frequently applied by the users of different disciplines of applied sciences and social sciences. In this chapter some more tests of significance are considered which have not been covered in those chapters. These tests are also based on t, F, Fisher’s z transformation,  and some other test statistics are also discussed one by one in the following sections.

13.2 t Test for Testing the Significance of an Observed Sample Correlation Coefficient r ^xy

Let r be the observed sample correlation coefficient based on a small sample of size n drawn from a bi-variate normal population with population correlation coefficient r. The standard error of r is given as

Q. 1 In building material RCC stands for
(A) Revised cost calculation
(B) Revised construction cost
(C) Reinforced central construction
(D) Reinforced cement concrete

New Technique of Food Preservation

1. High Pressure Processing (HPP)

High Pressure Processing (HPP) is a method of food processing where food is subjected to elevated pressures (up to 87,000 pounds per square inch or approximately 6,000 atmospheres), with or without the addition of heat, to achieve microbial inactivation or to alter the food attributes in order to achieve consumer-desired qualities. Pressure inactivates most vegetative bacteria at pressures above 60,000 pounds per square inch. HPP retains food quality, maintains natural freshness, and extends microbiological shelf life. The process is also known as high hydrostatic pressure processing (HHP) and ultra high-pressure processing (UHP).

Determination of True Protein

Apparatus

Wide mouth stoppered bottle, water bath plus those used for total nitrogen (crude protein) determination.

Reagents

1. Saturated solution of potash alum.
2. Stutzer reagent-Dissolve 40g of CuSO₄ in 2 litres of water in a wide mouth stoppered bottle. Add 10ml of glycerine and sufficient quantity of 30 % NaOH to make the solution alkaline (Litmus paper). Shake the contents and allow to settle. Discard the supernatant liquid. Collect the precipitate on a filter paper and transfer it to glass mortar with the help of spatula. Add 3ml of glycerine and a little water in the mortar and triturate the precipitate with a glass pastle. Transfer the triturated material to a wide mouth stoppered bottle, add 600ml of water, shake well and filter. Repeat the process till the precipitate (filtrate) is free from alkali and sulphate. Collect the precipitate and add 30ml glycerine. Dilute the emulsion to 320ml with water and preserve in a container.

Edaphology is the study of soil properties in relation to plant higher production.

Pedology refers to study of origin of soils in their natural environment, classification and description.

pH is the negative logarithm of the concentration of H+ ions in soil solution.

Soil profile is a vertical exposure of horizon sequence.

Soil texture is not changed by cultural management.

Cation exchange capacity (CEC) is defined as sum total of exchangeable cations that a soil can absorb.

A. First Aid
1. Abscess
What to Do

    ·    Abscesses should be examined by a veterinarian as soon as possible and within 24 hours.
 
    ·    Fluid may normally drain from the site of an abscess. An abscess does not drain through the tube, but rather around the latex tubing. Therefore, it is important for you to clean the area around the drain twice daily with warm water.

    ·    Apply a hot compress to the affected site at least two times daily for 3 to 5 days after the animal leaves the hospital. Wet a clean washcloth with very warm water and place it directly over the affected site, and then apply gentle pressure ideally for 5 to 10 minutes.

The understanding of VAM symbiont and VAM symbiosis is of prime importance in manipulating VAM associations for the welfare of plants. Many contributions have been made worldover in the areas of taxonomy, morphology, anatomy, histology, cytology and genetics of VAM fungi and/or their associations with the plants. However, the share of Indian mycorrhizasts in such contributions is negligible. The following description gives an account of work done by the mycorrhizasts of the country in these areas.

There aremany different points of view aboutthe nanotechnology. These differences start with the definition of nanotechnology. Some define it as any activity that involves manipulating materials between one nanometer and 100 nanometers. However the original definition of nanotechnology involved building machines at the molecular scale and involves the manipulation of materials on an atomic (about two-tenths of a nanometer) scale.

The debate continues with varying opinions about exactly what nanotechnology can achieve. Some researchers believe nanotechnology can be used to significantly extend the human lifespan or produce replicator-like devices that can create almost anything from simple raw materials. Others see nanotechnology only as a tool to help us do what we do now, but faster or better.

The third major area of debate concerns the timeframe of nanotechnology-related advances. Will nanotechnology have a significant impact on our day-to-day lives in a decade or two, or will many of these promised advances take considerably longer to become realities?

Finally, all the opinions about what nanotechnology can help us achieve echo with ethical challenges. If nanotechnology helps us to increase ourlifespans orproduce manufactured goodsfrom inexpensive raw materials, what is the moral imperative about making such technology available to all? Is there sufficient understanding or regulation of nanotech based materials to minimize possible harm to us or ourenvironment? Only timewill tell how nanotechnology will affect our lives.

Loss of experimental observations is a common practical problem in forestry and agricultural experiments because seeds, seedlings, plants, and trees may be damaged by natural events, animals, anthropogenic activities, or other causes. This chapter presents the missing plot technique for estimating unavailable observations so that an experiment can continue to be analysed. It explains why missing observations can reduce the information and precision of an experiment and discusses alternative approaches for handling incomplete data. The chapter focuses particularly on the method of least squares and the iteration method. Applications include estimation of missing values in Randomized Block Designs and other experimental layouts used in forestry research.

Perennial nature of horticultural fruit crops provides a stable environment for the buildup and development of mite populations, as against the ephemeral habitat of short-lived plants. The phytophagous mites are represented by 169 species whereas 148 species belong to the predatory group. Mites (subclass Acari) are ecologically and morphologically very diverse group of tiny invertebrates that belong to the class Arachnida (along with scorpions and spiders), subphylum Chelicerata under Arthropods. As arthropods also include insects, but mites are differ because they have four pair of legs (insects are hexapods), lack of true head and conspicuous segmentation of the body. 

There are several species in this cosmopolitan family that are of importance chiefly as pests of stored foods, flour, grain, seeds and bulbs, feeding on these products or the fungi that develop on them. These mites are mostly very minute, almost colourless, with the body bearing a few long setae, and with the legs terminating in one claw and usually a sucker. When very abundant the mites and their cast skins form a buff-colored dust and give rise to a characteristic odour. The living mites move slowly away from a source of light. An interesting characteristic of these mites is a nymphal resting stage (hypopus) during which they take no food but attach themselves to other mites, insects and humans by means of suckers and are thereby transported to other suitable places for development, where they drop off, moult, feed, and develop into adult stage. The hypopus may be resistant to fumigants. However, the cheese mites, Tyrophagus casei do not have a hypopial stage. Prolonged storage of products subject to infestation without cleaning of pantries and shelves where such materials are placed. These mites can subsist on organic debris in cracks, crevices and corners and are readily spread to other areas.

Mites are small arthropods belonging to the sub-class Acari (also known as Acarina) and the class Arachnida. The scientific discipline devoted to the study of ticks and mites is called acarology. In soil ecosystems, mites are favored by high organic matter content and by moist conditions, wherein they actively engage in the fragmentation and mixing of organic matter (Nyle and Ray, 2009).

Mites are small arthropods belonging to the subclass Acari (also known as Acarina) and the class Arachnida. The scientific discipline devoted to the study of ticks and mites is called acarology. In soil ecosystems, mites are favored by high organic matter content and by moist conditions, wherein they actively engage in the fragmentation and mixing of organic matter (Nyle and Ray, 2009). Mites are among the most diverse and successful of all the invertebrate groups. They have exploited an incredible array of habitats, and because of their small size (most are microscopic), go largely unnoticed. Many live freely in the soil or water, but there are also a large number of species that live as parasites on plants, animals, and some that feed on mold. It is estimated that 48,200 species of mites have been described (Halliday et al., 2000).

Mites have been known as pests of agricultural and horticultural crops since long but now they rank as very serious pests and vectors of many plant diseases. The application and extensive use of pesticides, fertilizer and improved cultural methods have probably favoured increase of the population. Mites as crop pests are confined to certain group of the other acari. Near about 20,000 species have been described of the total mite fauna on a world basis. From India, 660 species of phytophagous mites have been described and nearly 30 species are reported as potential pests (Gupta 1987). Phytophagous mite belongs to family - Tetranychidae, Tenuipalpidae, Tarsonemidae, Eriophyidae and Tuckerellidae. These mites cause direct damage like loss of chlorophyll, stunting of growth, defoliation, reduction in size and quality of fruits, appearance of various types of plant deformities etc. and all these severely affect the yield. Besides causing direct damages, few phytophagous mites inject toxic substances in to their hosts, which cause increased localized growth and disruption of tissue (Jeppson et al. 1975), and many species belonging to family – Eriophyidae are known to act as vectors of plant viruses. The dust entangled in the web formed by tetranychid mites on leaves prevent the sunlight reaching the leaf surface and as result photosynthesis is impaired, the leaves turn pale, yellow and fall down. This affects the vitality of the plant.

Introduction

Mites and ticks belong to acarology an offshoot of animal science Zoology a common man mites and insects look alike but former can be easily differentiated by absence wings and antennae and presence of four pairs of legs in most farms. Parasitic mites and ticks live on domestic animals, pets and poultry (Baker and Pritchard, 1960; Baker and Tuttle, 1994; Krantz and Walter, 2009). They are responsible for inducing paralysis, host intoxication, irritation carriers of pathogens and other health disorders to them. The severe bites of ticks cause tick toxicosis producing deep wounds, which bleed and are open to secondary infection. Parasitic mites are among the serious pests of the bees the world over. At times they cause mass mortality of brood and put a heavy loss to apiculture industry. Both solitary and social bees that are important pollinators are parasitized by Messostigmatid mites reducing their life span and foraging efficiency. They parasitize silkworm larvae, pupae and adults. By their toxication silkworms die in few hours. Man himself is not free from the attack of mites. Human itch mite, scabies mite and pyroglyphid mites causing allergic asthma are notorious examples of parasitic mites on man. Dancing on the head of a pin’ is definitely impossible for most animals. However, a large number of mites, the smallest Arachnida could do it. The size of these creatures ranges from a fraction of a millimetre, invisible to the naked eye, to about one centimetre in ticks.