eChapters

Cyanobacteria have been identified as one of the most promising groups of organisms from which novel and biochemically active natural products are isolated. Cyanobacteria such as Microcystis, Anabaena, Nostoc and Oscillatoria produce a great variety of secondary metabolites. The only group comparable to cyanobacteria is actinomycetes, which has yielded a tremendous number of secondary metabolites. The rate of discovery from traditional microbial drug producer like actinomycetes, which is in the focus of pharmaceutical research for decades, is decreasing and it is the time to turn to cyanobacteria and exploit their potential. Recently, there has been an increasing interest in cyanobacteria as a potential source of new drugs (Glombitza and Koch, 1989; Schwartz et al., 1990). This is of paramount importance to fight increasingly resistant pathogens and newly emergent diseases (Hayashi et al., 1994).

Cyanobacteria are photosynthetic microorganisms that have flourished on earth for more than 3 billion years with some genera showing only minor morphological changes since that time (Hagmann and Juttner, 1996). The medicinal and nutrient qualities of cyanobacteria were first appreciated as early as 1500 BC, when Nostoc species were used to treat gout, fistula and several forms of cancer (Pietra, 1990; Bladon, 2002; Liu and Chen, 2003).

Cyanobacterial species

Cyanobacterial species viz., Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were obtained from National Center for Conservation and Utilization of Blue-green Algae, Indian Agricultural Research Institute, New Delhi. Cultures were maintained in BG-11 growth medium throughout the study in the laboratory.

Culture Media and Reagents

The media used throughout the study were procured from Hi- Media Laboratories, Mumbai, India and the chemicals used were procured locally. Composition of each medium is as follows:

Nine cyanobacterial strains viz., Anabaena variabilis, A. fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were cultured in the laboratory. The growth curve for each culture was studied by growing them in Erlenmeyer flask using BG-11 growth medium. They were examined to determine the early stationary phases of their life-cycle, which are believed to be the most appropriate period for the production of secondary metabolites by an organism. After 23-25 days of incubation period, each culture was harvested and spent medium was collected by filtration of the biomass. Hexane, ethyl acetate, dichloromethane and methanol were used as the solvents for obtaining the extracts from biomass and spent medium of each culture which were then investigated against five pathogenic bacteria viz., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Antibacterial activity of different cyanobacterial sp. has been shown in various tables.

In the present investigation, nine cyanobacterial species viz. Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, Nostoc punctiforme, Nostoc linckia, Nostoc commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were tested for their activity pattern against some selected bacterial species.
 
Each cyanobacterial species was initially subjected to determine the early stationary phase of their life-cycle, which is believed to be the most appropriate period for the production of secondary metabolites by an organism. The growth curve for each cyanobacterial species was prepared by culturing them in BG-11 growth medium. Optical densities (OD) recorded at 650 nm were plotted against number of incubation days for each cyanobacterium. After 23-25 days of incubation period, each culture was harvested to obtain the biomass and spent medium, which were then subjected to process of extraction by using various solvents viz., Hexane, ethyl acetate, dichloromethane and methanol. The extracts and spent medium thus obtained were then investigated for antibacterial activity against five pathogenic bacteria including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnumoniae and Salmonella typhi.

Present work was aimed to determine the antibacterial activity and minimum inhibitory concentration (MIC) of active crude extracts of selected cyanobacterial species. Nine cyanobacterial strains viz. Anabaena variabilis, A. fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. belonging to family; Nostocaceae, Oscillatoriaceae and Stigonemataceae were selected from the herbarium of Indian Agricultural Research Institute, New Delhi, and maintained in BG-11 growth medium throughout the study in the laboratory.

The objective of the present investigation was to evaluate the antibacterial properties of cyanobacterial species viz., Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, Nostoc punctiforme, Nostoc linckia, Nostoc commune, Spirulina platensis., Westiellopsis prolifica and Hapalosiphon sp. against some pathogenic species including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnumoniae and Salmonella typhi. The following are the major findings of the study.

    •    Extracts prepared in methanol were found more active as compared to ethyl acetate, hexane and dichloromethane.
 
    •    Antibacterial activity of extracts varied with the test organisms. Maximum antibacterial activity was shown against Staphylococcus aureus followed by Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Klebsiella pneumoniae was found to be least susceptible against all tested cyanobacterial extracts.

    •    Antibacterial activity was observed against both Gram-positive and a spectrum of Gram-negative bacteria by almost all the cyanobacterial species examined in the present study except Westiellopsis prolifica where activity was observed only against S. aureus, a Gram-positive bacterium.

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Asthana, R.K., Srivastava, A., Singh, A.P., Deepali, Singh, S.P., Nath, G., Srivastava, R. and Srivastava, B.S. 2006. Identification of an antimicrobial entity from the cyanobacterium Fischerella sp. isolated from bark of Azadirachta indica (Neem) tree. Journal of Applied Phycology. 18, Number-1, 33-39.
Ayehunie, S., Belay, A., Baba, T.W., and Ruprecht, R.M. 1998. Inhibition of HIV-1 replication by an aqueous extract of Spirulina platensis (Arthrospira platensis). J. Acquir. Immune. Defic. Syndr. Hum. Retrovirol. 18, 7-12.

Abstract
 
The definition of quality is described from a very simpler one “fitness for purpose or use” to a comprehensive “the totality of features and characteristics of a product or service that bear on its ability to satisfy stated or implied needs”. The concept of total quality management in food industry is adopted recently and it is the “integrated organization approach in delighting customer (both external and internal) by meeting their expectations on a continuous basis through everyone involved with the organization working on continuous improvement in all products/processes along with proper problem solving methodology”. TQM is concerned with the integration of all the efforts in organization towards quality improvement, quality development and quality maintenance to meet full customer satisfaction at economic levels. TQM enhances the work culture and employee’s satisfaction through participation and involvement and consequently the image of the organization.

Abstract

Wheat is unique among cereals because of its ability to form viscoelastic dough. Variety of products such as leavened and unleavened breads, pasta, cookies, and breakfast cereals are prepared using wheat. The viscoelastic property of dough is mainly due to the presence of gluten proteins viz., gliadins and glutenins. Variations among these proteins play a significant role in determining the quality of wheat. Although considerable studies have been carried out on quantity and quality of protein and their relation to bread quality, very little work has been reported on their relation with quality of Indian traditional products like chapati and tandoori roti. Results indicate that ratio between gliadins and glutenins are important in determining the chapati quality. It has been found that varieties having 5+10 HMW glutenin subunits with IBL/ IRS chromosomes are more suitable for the preparation of chapati and roti. This article is focused on the relation between protein and its subunit composition and the quality of chapatti and roti quality.

Abstract

There are enormous food processing industries comprising of grains and oilseeds milling, fruits and vegetables processing, spice grinding and other food processing units in India. Also, there are many seed processing companies in the country, which are engaged in the processing and production of good quality seeds of different crops besides National Seeds Corporation and State Seed Corporations. All industries are using different type of machinery and equipment like cleaner, grader, expeller, milling machines, polishers, dehusking machines, size reduction equipment, different type of grinders, separators, treatment machinery, mixers, pulverizes etc. Besides this equipment, material-handling equipment like conveyor (belt, chain or screw type), elevators, holding bins and other handling equipment are also frequently used in the processing plants. To meet the above requirement many firms are also involved in the manufacturing of these equipment and machinery. Since, most of the seed processing equipment, dhal milling plants, material handling equipment are manufactured by the unorganized sector and hence equipment and machinery fabricated by them are not of very good quality. However, Bureau of Indian Standards has formulated the codes of manufacturing for these equipment and machinery. Seed processing equipment manufactured in India are generally copy of imported processing equipment. The manufacturers do not use materials of good quality. Framework of the machines is very poor. These machines are not also tested for their efficiency, 

Abstract

Mycotoxins are naturally occurring chemicals produced by fungi growing on grain, feed or food. The fungi that produce mycotoxins in grains commonly include Fusarium, Aspergillus and Penicillium species. The toxins can accumulate in maturing corns, cereals, soybeans, sorghum, peanuts and other food and feed crops in the field and in grain during transportation. The toxins may occur in storage under conditions favorable for the growth of the toxin producing fungi. Diseases in animals and human beings resulting from the consumption of mycotoxins are called Mycotoxicoses. These fungal metabolites are detrimental to the health of both animals and human. Toxicity ranges from acute death to chronic diseases, cancer and reproductive malfunction. Mycotoxin contamination of food and feed grains is a serious economic problem for grain producers and processors because grain contamination impacts market 

Abstract

Majority of basic research in our country has foundations in Universities and various Institutions linked to them. This paves way for discovering wilder varieties of the present known cereals and other food crops. Such findings help in bioprospecting the required gene/genetic material for the improvement of the existing crops. As per the Biodiversity Act of 2002, it is the responsibility of these institutions to document the same. But, as research scientists are geared to meet the challenges? Being used to socialistic system from the days of Independence where knowledge generated was open to everyone or closed and kept under the mountains of files, the TRIPS agreement has come as a shock. But the true impact of it was realized in 1999-2000, when the patent amendments were taken up. The private institution/ companies went into the act immediately.  We in the University neither were slow to react nor were we prepared to take the shock.  In this paper some of the problems pertaining to IPR and scenario of Indian Universities and Institutions in this regard were highlighted.

Abstract

Adulteration refers to a process by which the quality or the nature of the given substance is reduced by the addition of foreign or an inferior substance, removal of vital component, packing and storage under harmful conditions, insect infestation, poisonous material, undesirable chemicals and unintentional contaminants. All these change in food whether intentional or unintentional are covered under food adulteration. In cereal grains adulteration results by incidental contamination during the period of growth, harvesting, processing, transport, and distribution and results in various health hazard, sand, marble chips, earth and filth are also adulterants in grains. Pest and pesticides residues are also hazardous.

Abstract

Determination of quality of wheat is very important because quality requirement of an individual product vary from one another. Flour quality can be defined as the ability of the flour to produce a uniformly good end product. Flour quality factors can be divided into two basic groups: those that are inherent in the wheat as a result of the genetic make up and the changes brought about by growing conditions; secondly those factors that might be altered during the process of converting wheat into flour Wheat flour constituents play an important role in determining the quality of flour. The flour quality for baking could be assessed by measuring the physical, chemical and rheological characteristics.

Abstract

Wheat gluten consists mainly of gliadin and glutenin polypeptides. During mixing gliadin and glutenin proteins interact to form a unique viscoelastic gluten network, which is envisaged as being necessary for holding the gases and for producing a light porous crumb textured bread. Recent work has confirmed that the elastic properties of gluten are due to the glutenin fraction, whilst the viscous properties are attributed to the gliadin fraction. Equal amount of these two major protein components of wheat gluten is required for achieving the desired bread quality, but variation in the composition and physical properties of the glutenin polypeptides appear to be largely responsible for the differences in the gluten viscoelasticity and breadmaking potential among wheat cultivars. Recently, associations of gliadin polypeptides with breadmaking quality have also been reported. Using improved protein separation and purification techniques, physical methods and genetic engineering, we are beginning to understand the structure-functional relationship of wheat gluten proteins, but much remains to be explored in the years to come. 

Abstract

Consumers are now more conscious about quality and source of their foods. Attempts made to determine the quality of food materials are numerous, but most of them are destructive in nature. In recent years, nondestructive methods of quality evaluation have gained momentum and a considerable attempts have been made to develop them. This paper describes application of these non-emerging technologies: magnetic resonance imaging, x-ray computer tomography, near infrared spectroscopy and a few other important techniques for determination of different quality attributes with special emphasis on grain, and discusses their pros and cons for practical exploitation.

Abstract

Pulses being nutritionally vital, is an important constituent of diet for a very large number of peoples. A part from being a relatively cheaper source of dietary protein, it is also an important source of calories, certain essential amino acid, minerals and vitamins. Post harvest scenario of pulse shows that 75 per cent of production was milled in pulses with as dehusked split i.e. dal. Traditionally green gram and black gram dal is dehulled manually after soaking in water. Present investigation was carried out to develop the wet type dehuller and study was carried out on water absorption characteristics of green gram and black gram. The result reveals that the dehulling efficiency of abrasion type mechanism was about 95 per cent and 92 per cent in case of shearing type mechanism. It has been observed that rate of water absorption was initially high and then gradually decreases i.e. 0.54 to 0.18 and 0.48 to 0.18 for black and green gram, respectively. 

Abstract

The constant improvement in automation of bakery industry has placed higher demands on wheat. As a consequence bakery industry requires wheat possessing specific quality attributes. In view of this, there is need to produce good quality wheat not only for good chapattis but also for better bakery products viz., bread, biscuit, noodles, pasta products. New technologies are emerging which could be exploited for determining the Indian wheats for their suitability for various value added products. In the current investigation, 50 genotypes possessing low protein content (<10.5%) selected from a vast amount of breeding material were analysed for their biscuit making attributes. These lines were screened by actual baking test and alkaline water retention capacity (AWRC) test keeping the standard prescribed for biscuit making qualities viz., low protein content, low sedimentation value, low AWRC and larger diameter with larger spread factor of biscuit. Further with the help of Stable Micro Systems TA.HDi Texture Analyser the biscuits were analysed for hardness and crispness. Correlation coefficient among 8 quality traits suggested highly significant and positive correlation between sedimentation value and AWRC, biscuit diameter and spread factor, and biscuit hardness and fracturability, and significant negative correlation between biscuit diameter and its thickness. On the basis of findings, genotypes identified for biscuit making qualities may be used as donor for developing varieties suitable for biscuit making.

Abstract

The control of micronutrient deficiency is a realizable goal, notwithstanding the magnitude of the task, challenges and constraints that need to be resolved. Since wheat products particularly maida and atta form the major raw material in widely consumed food items like chapati, bread and biscuit. Fortification of flour as followed in many other countries could be an important strategy to help realize this goal. The technology of flour fortification is effective and cost affordable. Effective flour fortification program would require collaboration between scientific community, governmental and non-governmental agencies, and industry and consumer groups.

Abstract

30 lines were selected from 864 germplasm lines for good biscuit making attributes on the basis of low protein content (<10.5%) and low sedimentation value (<40cc). These lines were further screened by actual baking test and alkaline water retention capacity (AWRC) test keeping the standard prescribed for biscuit making qualities viz., low protein content, low sedimentation value, low AWRC and larger diameter with larger spread ratio of biscuit. AWRC had significant negative correlation with biscuit spread ratio. Positive significant correlation was found between biscuit spread ratio and biscuit diameter. On the basis of above findings, it may be recommended that low protein content, low sedimentation value, low AWRC may be used as criteria for better biscuit making i.e. increase biscuit spread ratio and biscuit diameter. So wheat-breeding programme has opened up new opportunities for the end use quality product and wheat based food industry. Hence there is an urgent need for qualitative analysis of desired traits in wheat towards enhanced value addition and thereby an attempted has been made to identify suitable genotypes possessing good biscuit making attributes

Abstract

Paddy seeds of variety PR - 116 were obtained from the PAU seed farm. These seeds were cleaned with commonly used seed processing equipment. Samples were divided into 2 parts. One part was kept as such for taking the observations of feed (control) and the remaining part was passed through specific gravity separator for its refinement. The various fractions obtained were recovery, middlings, and rejections. These graded fractions as well as the feed were packed in 5-kg cloth bags and stored under ambient conditions. Different quality parameters viz. moisture content, physical purity, thousand grain weight, percent germination, root/shoot length, vigour index and speed of germination were recorded at 30 days interval. The physical purity, thousand grain weight, percent germination, root length, shoot length and vigour index were found to be higher in recovery and minimum for rejections while moisture content was highest for rejection as compared to all other fractions. Germination, root/ shoot length, vigour index and speed of germination increased with the duration of storage while moisture content, thousand-grain weight and physical purity followed a reversed trend. Compared to feed, the recovery had higher quality attributes after 4 months of storage viz. moisture content decreased by 0.3%; physical purity increased by 0.67%; thousand grain weight increased by 2.97 gm/ thousand grain, germination increased by 8%; vigour index increased by 481.1 points; speed of germination increased by 3.2 seedlings per day. The grading resulted in improvement in overall quality of sunflower seed.

Abstract

Garden peas of two cultivars (Pb-87 and Pb-88) were procured. Pods were shelled manually, grains were blanched and dehydrated. Other lot of peas of both the varieties was allowed to mature on the plant till the plant foliage dried up, harvested, pods were dried in shade for few days, shelled manually and grains were further dried in shade. Grits were prepared by grinding rice and pea (dhal) in a laboratory grinder to pass through 20 mesh sieve. Blends were prepared by mixing rice and pea grits in the ratio of 100:0, 85:15 and 70:30. The blends were subjected to extrusion cooking using Brabender single screw extruder. The extruder die pressure, specific energy consumption (SEC), expansion ratio, density, water absorption index (WAI), water solubility index (WSI) and organoleptic characteristics were studied. Addition of pea grits resulted in a decrease in die pressure in all the blends. Addition of pea grits showed a significant effect on SEC. A decrease in expansion with increase in the level of pea grits in blend was observed. The extrudates having lower expansion showed higher density values and vice versa. Effect of replacement of rice grits with pea grits on WAI was found to be significant. Level of pea grits significantly affected the WSI. Incorporation of pea grits in the rice grits significantly affected the sensory characteristics. Replacement of rice grits with mechanically dried pea grits resulted in deterioration of acceptability score, but addition of field dried pea grits improved the same.

Abstract

India has become not only self sufficient in food grains but has also emerged as net exporter of wheat and rice. Acute famines in India have practically been eliminated, thanks to increased food availability at the national level, improved early warning systems and expanding infrastructure for more rapid transport of food grains and other products to needy areas. However, the existing post management system, strategies and policies don’t seem to match new challenges. Despite government intervention in pricing and procurement of food grains, primarily wheat and paddy, India continues to suffer heavily on account of losses and wastages incurred during the post harvest stages, which are more manmade than natural. Some losses have already occurred before the arrival of food grains in the regulated and wholesale markets and continue till food grains reach their destination. What contribute to continuing losses and wastages are improper post harvest handling, government policies and their implementation, which even stifle growth and modernisation of grain markets and processors.

Abstract

‘Made in India’ is no more a sign of poor quality. However, in the field of food grain exports, India is still lacking the required determination. The Indian food grains get rejected in the International market because of its out-dated post harvest technology (PHT). The Food Corporation of India (FCI) is the nodal national agency for the procurement, storage, processing and transporting of food grains, and their further distribution through the Public Distribution System (PDS). The government fixes the procurement price as well as the distribution price of the food grains, and the FCI has to accept it. The government compensates the resulting loss through subsidy. The government, through FCI, must encourage cultivation and marketing of premium quality food grains, by offering suitable incentives to farmers. The cost of marketing, storage and transporting of food grains can be considerably reduced with simultaneous improvement in quality of the end product, if the prevailing bag handling is replaced by bulk handling, storage, and transportation. However this needs closer coordination between various departments of the Ministry of Food and Ministry of Railways. The President of India revealed his vision to make India a developed country by the year 2020. To achieve this, the current post harvest losses to the tune of 10 percent must be reduced to 2 percent. This is possible if the government takes bold political decision supported by equally firm and time-bound necessary

Abstract

High moisture paddy can be dried in a trolley drier at farm level/grain market to reduce losses during various post harvest operations. During last two paddy harvesting seasons quality analysis of eight, most popular varieties of fresh paddy arrivals from about 40 grain markets, scattered all over Punjab, was performed. Monitoring of paddy in grain markets showed that maximum number of samples (62.7%) was in the range of 12-18%, moisture content (w.b.). Over-dried paddy samples (<12% m.c., w.b.) were only 9.1%. The samples with more than 18% m.c ,w.b. (high moisture paddy) were 28.2%, which have to be dried to safe moisture levels. Milling characteristics of the analyzed samples indicated that the broken percentage was highest (25.5%) in PR-113 followed by PR-111 (20.6%) and minimum in Pusa-44 (12%). The highest insect- pest infestation (Sitotroga cerealella (olivier) was observed in PR-108, followed by PR-116, PR-113, PR-111, Lal Mundi, Govinda, PR-114 and Sharbati. The Mycoflora isolated and identified were Aspergillus, Penicillium, Mucor, Rhizopus, Alternaria, Curvularia, Helminthosporium and Cladosporium spp. Maximum fungal infestation were associated with samples of PR-108 variety. To avoid further proliferation of mycoflora and inset pests, paddy should be dried to safe moisture levels for further storage and processing. For this, a trolley drier has been developed by converting a conventional farmer’s tractor trolley, into a portable drier, which can reduce 5% moisture of 1 tonne batch of paddy in about 100 minutes, consuming 56 kWh of electrical energy

Abstract

Sunflower seeds of variety PSFH- 67 were obtained from the PAU seed farm. These seeds were cleaned with commonly used seed processing equipment. Samples were divided into 2 parts. One part was kept as such for taking the observations of feed (control) and the remaining part was passed through specific gravity separator for its refinement. The various fractions obtained were recovery (heavier, healthy seeds), middlings (intermediate, under size) and rejections (lightweight seeds, wevil eaten, foreign matter). The respective percentages of each fraction were computed. These graded fractions as well as the feed were packed in 2-kg cloth bags and stored under ambient conditions. Different quality parameters viz. moisture content, physical purity, percent germination, thousand grain weight and vigour index were recorded at 30 days interval from Jan, 2010 to Apr. 2010. The physical purity, moisture content, thousand grain weight, percent germination, root length, shoot length and vigour index were found to be higher in recovery and minimum for rejections while moisture content was highest for rejection compared to all other fractions. The physical purity remained more or less constant throughout the period of storage. The thousand-grain weight showed a rapid decrease during first month thereafter it remained constant. There was continuous decrease in percent germination with the period of storage irrespective of the treatment.

Abstract

The establishment of All-India Coordinated Wheat Improvement Project in 1965 coincided with the introduction of high yielding semi-dwarf wheat varieties. Since then India has made very impressive progress in increasing wheat production over the past three and half decades. India now ranks as the second largest wheat-producing nation in the world. In fact from a state of “Ship to mouth” in 30 years or so India has become a nation of surpluses. The significant contribution made by the project in helping the increased wheat production cannot be ignored. Through coordinated research and development, nearly 200 wheat varieties have been developed during the last 35 years in India. However, only a small number of them produced without backing of specific quality breeding programs excel in quality traits also. The increasing demand of baked and pasta products in the country along with economic liberalization and global trade have offered opportunities for better utilization of the surplus wheat. Thus, in the current decade and beyond, wheat quality needs uppermost attention to meet the trade requirements for domestic and export purposes. Therefore, there is a need to reorient the wheat improvement programs in the country.

Abstract

Four bread wheat varieties (PBW-138, PBW-299, PBW-343 and PBW-373), two of durum wheat (PDW-215 and PDW-233) and two of triticale (TL-419 and TL-1210) were milled to obtain 70 percent extraction flour.  The grains and the flour samples of all the varieties were evaluated for proximate composition, rheological properties and pretzel making. Physical properties of durum wheat were better followed by bread wheat and triticale. Durum wheat were harder, had higher grain weight than bread wheat and triticale.  Protein, ash, fat, total sugars and enzyme activity were higher in triticale flour than bread and durum wheat flours while durum flour had higher pigments (ppm, b-carotene). Bread wheat flour was intermediate between durum and triticale flours with respect to fineness. Rheological studies revealed triticale flour to be the weakest flour. Pretzels prepared by using optimized recipe and method revealed PBW-343 and PBW-373 best for pretzel making.

Abstract
 
Technological advancement, increased urbanization, awareness and interest of consumer for variety products have given a great challenge to the food sector to develop inexpensive foods that are nutritionally superior and acceptable to the intended consumers. Keeping this objective in view, we have made an attempt to develop technology for variety products from wheat and corn and suitability of available varieties judged. With the emerging trends of food market towards fast foods, there are vast prospects for efficient utilization of cereals by exploring varieties products. A steamed bread (a Chinese traditional product) and Tortilla and corn chips (Mexican snacks) were developed. Steamed bread is defined as the product resulting from cooking or steaming fermented dough. Recipe and method for its preparation was standardized. Nutritionally rich steamed bread can be prepared by supplementing 10% level of chickpea and defatted soybean flour. Corn snacks have become a prime target of some consumer advocates and nutritionists who have referred them as a source of ‘empty calories’. But reality is that these snacks provide instant energy, as they are rich storehouse of nutrients. Process and recipe for the preparation of Tortilla and corn chips from Indian maize were standardized. The quality and acceptability of these snacks were worked out and found that these products were highly acceptable. Indian consumers have versatile taste, so these products will be readily accepted in Indian market. 

Abstract

Three-point bend test (three point bend ring-HDP/3PB) for five types (Good day, Butter bite, Marie, Krack Jack and Salted) of commercial biscuit brands (Britannia, Supreme, Parle, Lancer, Priya Gold and Dalmia) was developed using TA-XT2, texture analyzer and probable factors affecting the results were assessed. Maximum force to break the biscuits, number of fracture before break, thickness of biscuits and percent break point penetration were found in the range of 1006.5 to 3456.5g, up to 3, 4.2 to 6.3mm and 5.54 to 22.62%. The rupture of biscuits were observed on the basis of probe penetration into the biscuits and found that maximum resistance for penetration offered by Marie whereas minimum by salted biscuits, which was respectively the hard dough and fermented dough biscuit varieties. The analysis of variance for the results revealed that there is significant difference (P£0.01) in the maximum force of break for the biscuit types and brands individually or in combination. Difference in the thickness of biscuits was also found significant among types and commercial brands. The number of fractures was not consistent among the types as well as biscuit brands before reaching the maximum force of breaking the biscuit.

Abstract

The demand for the food for ever increasing population is always continuous, but the production is limited to specific seasons. Therefore the food grains have to be stored for sometimes. Grain is a living biological material and therefore storage structures should be suitably designed to protect them from the ravages of pests, rodents and micro-organisms. However, the storage losses account for a major portion which can be reduced to considerable extent if proper care is done. The faulty design, poor maintenance and limited knowledge of the farmers concerning storage technology have greatly added to the spoilage of the food grains during storage. Sometimes the foods if not properly stored may be edible but it may lose the essential vitamins and minerals, thus their nutritive value has been lost. There is a major requirement of a good storage structure. Various food grain storage structures (small and large scale) are described.

Abstract

Maintaining grain in good condition in storage requires careful, routine inspection and good storage practices. An integrated approach of several management tools and practice factors are necessary to manage stored grain to prevent deterioration and wastage: This includes:1) harvesting, handling, and storage equipment; 2) initial condition of the grain; 3) grain moisture content; 4) grain temperature; 5) aeration system management ; 6) insect and mold control; and 7) monitoring grain condition. An effective management program greatly enhances the likelihood of maintaining top quality grain. A well- designed and properly operated storage system with adequate aeration capacity, storing only clean grain at the proper moisture content and temperature, sanitizing the bin before loading, checking the grain condition regularly and correcting problems before they get out of hand are critical management strategies that must be implemented to prevent grain damage and possible serious economic loss.

Abstract

Pulses constitute an important component of the diet and are a major source of protein in vegetarian diet. India has largest area under pulse cultivation and produces a variety of pulses. A large portion of this produce gets spoiled due to insect and fungal attack. A variety of insecticides and fungicides are used for the control of pest during storage. There is an increasing trend towards the reduction in the use of pesticides in storage due to strong demand from consumers to choose food with no pesticide residue, development of insect resistance to pesticides and the impact of pesticides’ use on environment. All these have posed a challenge to refine the existing management procedures of storage and commodity to find the optimal solution. Controlled/ Modified Atmosphere storage offers an alternative to the use of pesticides extends storage life and maintains product quality. The Controlled/Modified Atmosphere is created by altering the composition of the atmosphere in terms of CO2, O2, and N2 concentration. High CO2 produce undesirable conditions for insects and microbes and thus control their activity.

Abstracts

About 60-70% of total food grain production is retained and stored by farmers for household consumption, use as seed, feed for cattle and payment of wages to labours etc. in our country. Yet the quality of grains stored is not so good, and hence, preserving the harvested crops without loss or damage has always been a problem of national importance. This study was conducted in Jabalpur district of Madhya Pradesh state. A tribal block namely Kundam was selected for study purpose. The responses of 120 women were recorded in pre-tested structural schedule through interview. Rural women are an important source of information on the use of indigenous practices for grain storage. The data reveals that the majority of respondents are using dry leaves of Neem, dry onion bulb, wheat straw, Nirgudi leaves, roots of bach etc. for storage of grain at household level. In our country, indigenous practices of grain storage have always been and are still followed, because it is supposed that these practices are useful in control of grain loss.

Abstract

There is an urgent need to – create market driven linkages rather than do it alone by building new infrastructure. A lot needs to be done to improve infrastructure in terms of reducing the logistics cost, eliminating middlemen influence and eliminating the wastage of basic inputs. There is a need to blend technologies based on principle of dovetailing production for masses with the advantage of mass production, particularly in the area of grain processing, packaging and marketing which would help combine livelihood, security with agricultural competitiveness. Risk in crop production is not new to any age and the farmers have been bearing it since times immemorial. The farmers should be allowed to maximize their grain. High performance continuous and batch dryers remove moisture in a way that is predictable and reliable. The problems associated with moisture content may be harmful to international trade in grain and need to be addressed adequately by the standardization institutions.

Abstract

A survey of various wheat milling units (atta chakki) available around Indian Agricultural Research Institute, New Delhi was conducted to study the performance of these. The mills of various sizes (12", 16", 18", 20", 24" and 30" stone disc diameter) with horsepower ranging between 2 to 20 are in operation. Both horizontal and vertical disc types of mills are in use. The capacity of these units ranges between 10 kg/h to 200 kg/h. The stationary sieve, hand sieve, pedestal/exhaust fan are used for cleaning. The average wheat cleaning cost is Rs. 200 per ton. A small room of 8' x 10', 10' x 10' and 10' x 12' size is used for running the mills and storage of wheat grains. The grinding charges (Rs./q) of wheat, coarse grains (maize, sorghum, millet etc) and spices are 100, 200 and 1000, respectively. Sample of 500 g of wheat and flour were collected from each chakki and were analysed for wheat grain and flour quality. Infested, damaged grains were observed to be in 2-10% range. Sieve analysis of the flour was done and the average particle size, fineness modulus and uniformity index of the flour were calculated. Average particle size, fineness modulus and uniformity index were found in the range 0.1875 to 0.2023 mm, 1.052 to 1.297 and 0:1:9, respectively. Average particle size of the flour obtained from horizontal mills was found to be higher than vertical mills. Temperature of the flour at the outlet was observed in between 45 and 50°C at room temperature of 18.5°C.

Abstract

Grains in present context are food grains. The PFA Act includes cereals, millets, and pulses in food grains. At times, seeds of certain oilseeds like soybean are also referred to in the discussion of food grains. Grains have been cultivated for thousands of years. We have Grain Standards as established by the International Organization for Standardization (ISO), which are considered as International Standards. Another source is Codex Alimentarius Commission (Codex) under the aegis of FAO (UN), which operates a Committee to formulate standards that are referred to as Codex Standards. Many countries have a National Standards Institution, which develops National Standards for their main grain crops and other commodities and issues specifications along with methods of testing and analysis. Several countries, which are in developing status, adopt or modify international standards into their national system. The Grain Standards have been evolved and established for a variety of purposes but primarily to grade produce in agricultural marketing largely to facilitate the movement of grain, provide both sellers and buyers with guidelines to support financial transactions, and ensure that the produce will meet up with end-use requirements, or, increasingly to protect the consumers.

Insects came into existence in this world some 250 - 500 million years ago, while man appeared in this planet only about a million years ago and intruded into the domain of insects. From this time onwards, a rapport had started between the insects and man. Man considered insects obnoxious and not worth consideration in the early days. However, the real impact of insects on the activities of man became manifested to a greater extent only during the past few thousands of years. Gradually man began to understand the nature of insects and started looking at insects, both as his adversaries and as his beneficiaries. He started exploiting the useful insects for his own benefit and tried to destroy those, which are harmful to him.
 
There are numerous references in the ‘Ancient Epics’ and ‘Puranas’ such as, ‘Ramayana’, ‘Mahabharata’ etc. to show man’s acquaintance with insects even from very early days. Several references to insects are found in the ‘Holy Bible’ also. Ancient people of India were quite aware of insects, which provide honey, lac and silk. They were familiar with honeybees and gathering of honey and bee wax from hives. Honey was used in most of the religious rituals. Honey and bee wax were used in many Ayurvedic medicines against various diseases. The story of the lac palace constructed by the Cauravas to destroy the Pandavas by setting the wax palace on fire at night as narrated in Mahabharata is quite well known. The ancient Indians knew how to manufacture silk from silk thread produced by silkworms. Records show that an Indian king had sent some silken stuff to a Persian ruler even as early as in 3870 BC. References to insects such as, flies, beetles, bees, wasps, moths, ants etc. are found in old Sanskrit literature. The Ancient people also used some Mercury, Arsenic and Sulfur compounds for the control of some of the harmful insects. Numerous references to insects are found in the ‘Holy Bible’ also. King Solomon, the wise (971 BC), who ruled Israel had told his people to learn from the ways of ants, which are so active and orderly (‘Proverbs’, Chapter 6). He had mentioned as to how the ants gather and store food at the time of harvest, when food is available in plenty to be used in summer when food is scarce (‘Proverbs’, Chapter 30).

‘Integument’, the outer layer of insects consists of the ‘basement membrane’, ‘epidermis’ and the ‘cuticle’. One of the most important characteristics of insects is the presence of the rather hard, flexible chitinous exoskeleton or cuticle, which covers the entire body and provides support to the body. The exoskeleton of insects that is akin to the internal skeleton of vertebrates accounts for their size and shape. Movement is made possible by the division of the cuticle into separate plates or sclerites.  Primarily these  sclerites  are confined to segments and the sclerite of one segment is connected to the sclerite of the adjoining segment by means of an articulate called ‘suture’, a region in which the cuticle is very thin and flexible. The cuticle of each segment is divided into four primary sclerites viz., a dorsal tergum, two lateral pleura and a ventral sternum. The dermal glands present in the epidermal layer secrete the entire cuticle of the insect.

Like that of the body, the cuticular skeleton of the appendages is divided into tube-like segments connected to one another by articular membranes, thus creating a joint at each junction. Such joints enable the segments of the appendages, as well as those of the body to move freely.

In addition to the exoskeleton, an endoskeleton is also developed. This may be an infolding of the procuticle that produces inner projections or ‘apodemes’ equal to bones in vertebrates on which the muscles are inserted.

Similar to animals of the higher order, insects also possess all important internal systems of organs necessary for the proper performance of the various vital functions. The most important of the systems are the alimentary or  digestive  system,  the  circulatory  system,  the  respiratory system, the reproductive system and the excretory system. The fundamental arrangement of the various organs is more or less similar in all insects and does not exhibit much remarkable variations as seen in the external morphological features. The dorsal blood vessel or heart runs along the median line immediately below the dorsal wall. The digestive system including the alimentary canal and its appendages lie in the middle, below the heart. The nervous chain lies below the alimentary canal and immediately  above  the  ventral  wall.   The  organs  connected  with  the reproductive system are found in the posterior region of the abdominal cavity on both sides of the alimentary canal.  Though these arrangements are characteristic of all Arthropods, including insects, they are quite different in the higher animals (Fig.23)

Insects generally reproduce by laying eggs or ‘ova’. The initiation of growth inside an egg, which in most cases is preceded by fertilization, leads to a long series of changes, finally attaining the adult stage and in due course to sexual maturity and finally to reproduction of a new generation. This chain of events from the egg stage to death constitutes the ‘life history’ or ‘life cycle’ of an insect.

Eggs are laid singly or in batches of only a few or up to hundreds or  even thousands. Generally eggs are laid under conditions, where food is available for the feeding of the future young ones.  The eggs are generally smooth, elliptical or oval, but some have diverse shapes such as, disc-like, hemispherical, conical, globular, spindle and many other forms. The egg surface may also have a variety of textures. In some aquatic insects, the eggs are covered with a gelatinous secretion, which swells in water forming a jelly-like spawn. In mosquitoes, the lateral sides of the eggs are expanded, which serve as floats. Eggs vary in size, from microscopic to about 3.0 mm. in diameter and 5.0 - 6.0 mm. or even more in length.

The development of an insect is a continuous process, but on the basis of external manifestations, it is divided into definite stages. The stage within the egg is termed ‘embryonic development’, that after hatching ‘post-embryonic development’ and the changes of form during the post-embryonic development constitute ‘metamorphosis’.

Protection from natural enemies and adverse environmental and climatic conditions is vital to safeguard insects and to this effect, insects possess a great variety of protective structures and mechanisms without which they cannot survive.

    i)    Food. Each insect species has certain nutritional requirements for the completion of life cycle. If the required food for any particular species gets reduced in a particular locality, that species will gradually disappear from that locality, though other environmental conditions may be favorable. Under normal conditions, there may be sufficient food for the insect to live on and continue its race. In the event of sudden increase in the population of an insect, the density of the population becomes too high to be supported by the food available in the area. Under such conditions, some individuals of the species either migrate to other suitable areas where enough food is available or else starve, if the species has limited power  of  dispersal  as  in  the  case  of  many  coccids.  According  to  the nutritional requirements insects are characterized as ‘omnivorous’ (feeding on different kinds of both plant and animal food), ‘carnivorous’ (feeding on animal food) and ‘herbivorous’ or ‘phytophagous’ (feeding on living plants). Those insects, which feed on many cultivated and wild species of plants, are termed ‘polyphagous’ (e-g) Locusts, grasshoppers, cutworms etc. If the feeding activities of a particular species of insect are confined only to plants of one botanical family, it is termed ‘oligophagous’ (e-g) cabbage butterfly - Pieris brassicae. When only a single species of plants constitute the food of a particular species of insect, it is termed ‘monophagous’ (e-g) Brinjal fruit borer.

The classification of living organisms has passed through several changes and has reached the present stage with the gradual assimilation of more and more knowledge about them. The classification is mainly based on the anatomy, life style, habits, habitats, metamorphosis and such other characteristics. Though the concept of classification of living organisms was perhaps started by Aristotle between 384 - 322 BC, it was the Swedish scientist Carl Linnaeus (1758) who presented the classification, which was acceptable to most of the Taxonomists. He propounded the binomial nomenclature of naming each and every organism of animal and plant origin with a generic and species name. In his classification, he was consistent in using Latin names for all the organisms. He was the originator of a regular system of classification in which he recognized larger and smaller categories, the larger ones comprising of a varying number of smaller ones. Because of his contribution in the field of Taxonomy, he is rightly called the ‘Father of nomenclature’. A.D.Imms later modified the system to some extent in 1961. However, Brauer (1885) laid the foundation of the modern system of classification of insects. He was the first one to emphasize the fundamental division into primitive wingless insects, the ‘Apterygota’ and the winged or secondarily wingless insects, the ‘Pterygota’. The latest code on classification and nomenclature was approved and published by the ‘International Trust for Zoological Nomenclature’, London in 1964.

One of the chief aspects of Entomology is the study of insects from the standpoint of their innumerable relations and activities affecting the interests of man. More than any other living organisms, insects have more intimate and complex relationship with man. It is well known that insects affect human interests both for good and bad. However, their injurious activities are of much greater concern to man than the beneficial aspects of their activities. Several insects are beneficial to man in several ways, both directly and indirectly and there are scores of other insects, which do not interfere with the interests of man in any way.

From the time man stepped into the domain of insects, a stifle has started between the insects and man. To meet his various demands for food, clothing, shelter and various other requirements, man cultivates various crops and raises many domesticated livestock. Unfortunately for man, insects also have started depending on these for their living. When the requirements for man and insects happen to be the same, a race is on for their survival and existence. Insects attack plants and livestock and cause extensive damage and economic loss. So, man considers such injurious insects as his enemy and to safeguard his interests tries to destroy them by various means. However, man’s supremacy and intelligence have not proved a match to the rapid propagation of insects and their various adaptations to withstand any hardship and adverse conditions. In the effort to gain an upper hand, it has not been possible for man to destroy the unwanted insects completely and the struggle is going on as a never-ending ordeal.

Though many of the insect species are injurious to man, his crops and his livestock in different ways, not all the insects are injurious. Some insect species are beneficial to man in many ways.  A  few  are  productive insects and yield materials directly useful to man. Of these, the most important ones are the honeybees, which give honey and bee wax, the silkworms, which give silk and the lac insects, which give lac. Some other insects are useful to man indirectly in many ways.

productive insects

1. Honeybees (Apis species)
Order - Hymenoptera
Family - Apidae

Kinds of honeybees

There are five kinds of honeybees in India. They are :
Apis dorsata - the rock bee
Apis florea - the little bee
Apis indica - the Indian bee
Apis mellifera - the European bee
Melipona iridipennis - the dammer or stingless bee

Collection and preservation of insect specimens is the best way to study their morphology, habitat, distribution, host relationship and many other interesting features. Killing, mounting and arranging the insects in some particular sequence creates much more interest than merely studying museum specimens or looking at pictures. A collection of varied types of insects goes a long way in increasing the knowledge and understanding of the taxonomy of insects.

Not all insects are found in every season and in every place. Their presence mostly depend on the availability of the host, as well as the climatic conditions. Though many insects can be collected in any season, they  are  naturally  abundant  in  moist,  warm  weather,  especially  in the  spring and early summer. To get a good collection, insects have to be collected continuously in all the seasons of the year, from all plants and plant parts, from stored grains and food products. Several types of butterflies, moths, bees, wasps, beetles and flies can be collected from flowers, which they frequent to collect nectar. Many insects can be collected from leaves, stems, fruits, roots and such plant parts. Many insects are found in water and on water plants. Some are found in barks of trees or in decomposing organic matter. These insects have to be collected from different localities where they are found. Most of the insects are diurnal, while some of them are nocturnal. Some are attracted to light. So, a knowledge of the habits of insects is important in making a good collection. Sometimes better specimens may be obtained by collecting the immature stages and rearing them into adults wherever possible. Keeping records of pest collections may help Entomologists to forecast largescale pest occurrence.

Insect pest control includes all measures adopted to make the life of insects hard and ultimately to destroy them or to prevent their multiplication and spread. Such measures can be taken up before the appearance of the pests or after occurrence of the pests. To achieve this purpose, it is quite necessary to know the factors which favor the occurrence of the pests and acquire enough knowledge about the nature and life cycle of the pests, the vulnerable growth stage at which they can be effectively destroyed and appropriate measures of control. Control measures may be either preventive or direct.
Insect pest control is broadly classified under two different heads viz., natural control and applied control.

I. Natural control
    1.    Climatic conditions. Temperature, rainfall, sunshine etc.
    2.    Physical or topographic factors. Mountains, oceans, rivers, lakes, forests, deserts etc.
    3.    Natural enemies. Parasitoids, predators including insects, birds, mammals, reptiles etc.
    4.    Disease causing organisms or parasites. Entomophagous fungi, bacteria, viruses and other microorganisms.

The chemicals, which can destroy pests or prevent their occurrence by their chemical action, are known as ‘pesticides’. Pesticides include ‘insecticides’, ‘acaricides’, ‘nematicides’, ‘rodenticides’, ‘molluscicides’ etc., which are used to destroy insect pests, mites, nematodes, rodents, slugs and snails respectively.

Pesticides have been used even before the time when man never knew how to read or write. Even before 200 BC, several substances were known to control pests. Sulfur, arsenic poisons, fluorine poisons, as well as some plant extracts and oils were used to control pests. However, these substances could destroy only a few specific pests.

Advances in the invention of pesticides

The invention of many of the present day pesticides started only after 1867. Paris green, a synthetic chemical was the first pesticide used to control the Colorado beetles of potato.  Up to  1939,  only  a few inorganic  chemical compounds and plant poisons were used as pesticides. Only after the Second World War much advances were made in chemical research. After the invention of DDT in 1939, a revolution had started in the field of pest control.  Following the introduction of DDT,  several other pesticides were identified and introduced for pest control. In 1941, BHC was invented by the British and French scientists. During the same period, organophosphorus pesticides were invented by the German scientists and parathion, malathion, demeton etc. were introduced.

For effective and economic control of pests, besides selection of appropriate plant protection chemicals, application of the chemicals at the most vulnerable growth stage of the pests and use of chemicals at the correct dosages, selection of proper plant protection appliances to carry out the operation is also of vital importance so as to ensure uniform application of the chemicals.
 
Plant protection chemicals are mostly used as sprays and dusts using sprayers and dusters respectively. However, in most of the cases spraying is preferred to dusting. Different types of sprayers and dusters are being used for these operations and in both, there are manually operated as well as power operated equipments and in sprayers there are both hydraulic and pneumatic types of sprayers.

The nature of the pesticide formulations to be used and the purpose for which they are used also determine the type of equipment to be used. Dusters are used for dusting insecticidal dust formulations; sprayers are used for spraying insecticidal liquid formulations; granular applicators are used to apply granular formulations; fumigators are used to apply fumigants to fumigate godowns, warehouses, rat holes etc.; soil injectors are used to inject pesticides at a particular depth of soil under pressure; flame throwers are used to throw flames at specified targets.