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Amino acids are the building blocks of proteins. They occur in all living plant cells as constituents of proteins and as free acids or amides. More than 200 naturally occurring amino acids have been identified in higher plants. Around 20 different amino acids have been observed to be incorporated into proteins. They have two functional groups, an amino group (-NH2) and a carboxylic group(-COOH). Both these groups are attached to the a carbon atom only. Amino acids with this structure are called a amino acids or 2-aminoacids. The unique characteristics of the 20 amino acids are due to the side chain (R), which can be an alkyl, hydroxyl, thiol, amino, sulphide, aromatic, or heterocyclic group.

Amino acids have the general formula, R-CH(NH₂)-COOH. The R represents a side chain. If R=H, the simplest amino acid glycine (NH₂ -CH₂ –COOH) results. Amino acids are alpha (a ) amino carboxylic acids. The carbon atom is tetrahedral in shape. The various groups attached to it are placed in different positions. Since the valence of the carbon atom is four, four groups can be attached to the carbon atom. Based on the groups attached to the carbon atom it may be of two types.

An amine reacts with a carboxylic acid to form an amide. Similarly, the amino group  on one amino acid molecule reacts with the carboxyl group of another amino acid  to form a compound called peptide. A peptide bond is an amide bond that forms when the –COO- group of one amino acid reacts with the -NH₃+ group of another amino acid. The resulting compounds are called peptides. Even after this there is a reactive amino group and a carboxyl group in the peptide. If these groups react further a poly peptide can form and later a polymer called protein can form.

The word protein was first coined in 1838 to emphasize the importance of this class of molecules. The word is derived from the Greek word proteios which means “of the first- rank”. Proteins are polymers/macro molecules made up of several amino acids. When there are more than 50 amino acids in a chain, the polypeptide is normally called a protein. Each protein in the cells of living beings has a unique sequence of amino acids that determines its biological function. The proteins are folded into specific defined structures, which are maintained by a large number of relatively weak bonds. The  three  dimensional structure is well defined and suited to it’s specific function.

Very small changes in the structure can modify the function. Hence, it is important to know the structure of proteins to understand their behavior and function. The biological activity of proteins depends on maintenance of folded conformation. Proteins fold into well defined three dimensional shapes and they are able to recognize their corresponding substrates or antigen molecules and bind them tightly. The protein structure has been classified into four different levels based on the folded conformation of the protein.

Enzymes are organic catalysts produced by living organisms. They make possible biochemical reactions. Hence they are called biocatalysts. They are mostly a specialized class of proteins. However, there are some non-protein enzymes like ribozymes, which are RNAses. Enzymes speed up reactions at ambient temperatures. This is in contrast to chemical reactions like saponification of lipids which take place when lipids are boiled for a few hours with concentrated alkali.  Enzymes, namely, lipases, hydrolyze lipids at body temperatures in minutes. They are superior to chemical catalysts by a factor of 107 to 1014 .  An enzyme may be a simple protein or a complex protein. They are produced by cells and are mostly globular proteins. Each cell contains an estimated 3000 different enzymes.

Nucleic acids were first discovered in 1868 by Friedrich Meischer. Nucleic acids are high molecular weight polymers that are present in our cells. They  store and transfer genetic material from generation to generation. Knowledge of how genes are expressed and how they can be manipulated is becoming increasingly important for understanding   of nearly every aspect of biochemistry. These macromolecules pass on information for cellular growth and reproduction.  All the genetic information in the cells is called the genome. Every time a cell divides, the information is copied and passed on to the new cells.
 
Nucleic acids fall into two main classes according to the type of sugar they contain: the Deoxyribonucleic acids (DNA) and Ribonucleic acids (RNA). Some sections  of  the  DNA  called  genes contain the information to make a protein. RNA translates the genetic information in the DNA to the ribosomes, where the synthesis of proteins takes place.

Plants are responsible for highly useful molecular reactions. All the enzymatic  reactions  that  occur in the cell are  termed  as metabolic reactions.  They  take place within organisms in an orderly and regulated way.  They  are  the  metabolic pathways and the compounds  resulting from the process, namely,  metabolism are called  the metabolites. Metabolism in Greek means change. It is the set of life sustaining chemical transformations within the cells of living organisms. These enzyme-catalyzed reactions allow organisms to grow and reproduce, maintain their structures, and respond to the environments around them.
 
Metabolism is a highly organized,   coordinated and purposeful enzymatic activity inside a cell. Metabolism has four functions: 1. To obtain energy from the environment, 2. To convert molecules into building blocks, 3. To convert the building blocks into proteins, lipids, sugars, nucleic acids and other cell components and 4. To synthesize and degrade organic molecules for specific needs of the cell.

Carbohydrate metabolism in the cell is essentially the metabolism of glucose and other substances related to glucose in their metabolic processes. The chemical reactions involved in carbohydrate metabolism represents complex groups, sequences, and cycles of reactions which integrate at various points with the reactions concerned in the metabolism of lipids and proteins. The major types of chemical processes involved in carbohydrate metabolism may be grouped as follows: a) Anabolism of starch – Photosynthesis, b) Catabolism of starch, c) Catabolism of glucose – 1. Glycolysis and TCA cycle and 2. Oxidative pentose   phosphate pathway.
 
Plant cells “burn “ glucose for energy. Plants also produce sugars through photosynthesis. This is roughly the reverse of the burning process.

The importance of lipids have already been elaborated. They play a major role as source of energy and as components of membranes which have implications in plant growth regulation. The complete metabolism of fat leads to oxidation, the final products being CO₂ and water. The burning of one mole of palmitic acid gives 2340 kcal.  The molecular weight of palmitic acid is 256. This works out to approximately 9 kcal/g of palmitic acid. This energy is twice that derived from either sugars or proteins. Fats and oils are thus better and more efficient forms of storage of energy in living beings.

Plants can synthesize all the 20 amino acids, which include essential and non-essential amino acids. They can be degraded by removal of the amino group and conversion of the remaining skeleton to useful metabolic intermediates which participate in the synthesis of glucose and other ketone bodies.
 
Biosynthesis of amino acids
 
The biosyntheses of amino acids have an important feature. Their carbon skeletons come from intermediates from glycolysis, pentose phosphate pathway and citric acid cycle etc. The biosynthetic pathways depend on the intermediates from which they are derived. The primary amino group normally comes from the transamination of glutamate. Glutamine synthetase is a central control point in nitrogen metabolism, since glutamine is the amino group donor in the formation of many biosynthetic products as well as being a storage form of ammonia. Glutamate is the precursor of proline, ornithine, and arginine. Serine, cysteine, and glycine are derived from 3-phosphoglycerate. Glycine is synthesized from serine by removing CH2OH group. Tyrosine is synthesized from phenylalanine. Asparagine and glutamine are amides that are synthesized from aspartate and glutamate.

Amino acids serve as the building blocks of proteins and as precursors of many other important biomolecules such as hormones, purines, pyrimidines, porphyrins and some vitamins. They also serve as sources of energy when present in excess amounts and when they are used as fuel, amino acids undergo deamination reactions. The remaining carbon skeleton will either convert itself into glucose or undergoes oxidation to CO₂ via TCA cycle.

Central dogma: The Central dogma of molecular biology, which has been formulated as a simple linear progression of information from DNA to RNA to Protein, is summarized in the illustration. The replication process on the left consists of   passing  information from a parent DNA molecule to daughter molecules. The middle  transcription process copies this information to a mRNA molecule.  Finally, this information is used by the chemical machinery of the ribosome to make polypeptides.

Primary metabolism in a plant comprises all metabolic pathways that are essential to the plant’s survival. Primary metabolites, namely, carbohydrates, lipids and proteins are compounds that are directly involved in the growth and development of a plant whereas the secondary metabolites are compounds produced in other metabolic pathways that, although important, are not essential to the functioning of the plant. However, secondary plant metabolites are useful in the long term, often for defense purposes, and give plants characteristics such as color.
 
Secondary plant metabolites are used in signalling and regulation of primary metabolic pathways. Plant hormones, which are secondary metabolites, are often used to regulate the metabolic activity within cells. Secondary plant metabolites help the plant maintain an intricate balance with the environment, often adapting to match the environmental needs. Plant metabolites that color the plant are a good example of this, as the coloring of a plant can attract pollinators and also defend against attack by animals. Terpenes, alkaloids, sterols, pigments, growth regulators, and phenolics are the important secondary plant metabolites.

Phenol is normally a benzene ring containing an –OH group, and hence an aromatic alcohol. The term phenolic refers to similar aromatic compounds with one or more -OH groups. Phenolics constitute one of the most widespread classes of secondary metabolites. Commonly talked about class of compounds like anthocyanins, flavonoids, lignins, tannins, coumarins and chalcones are all phenolic substances. Phenolics are water insoluble substances. However, the water solubility increases with the increase in the number of -OH groups. Phenolics absorb electromagnetic radiation strongly. Many of the phenolics absorb in the visible part of the spectrum (Ex. anthocyanins) and are coloured. Phenolics are produced in plants by 1. Shikimate pathway through phenylalanine (C6.C3)  and 2. Acetate/ malonate pathway.

Plant pigments impart colour to the different components like leaves, flowers, fruits etc. They in turn are responsible for the colour of raw food, we eat. Pigments like chlorophyll, anthocyanins, flavonoids, tannins, xanthones etc. are the different pigments that are present in plants. The primary function of pigments in plants is photosynthesis, which uses the green pigment chlorophyll along with several red and yellow pigments that help to capture as much light energy as possible. Other functions of pigments in plants include attracting insects to flowers to encourage pollination.

Sterols are derivatives of a complex ring system called cyclopentano perhydro phenanthrene. The term sterol means solid alcohol. Sterols, though alcohols, are solids with a M.P. of 100-120^ºC. The 27 carbon atoms impart hydrocarbon character, namely, solubility in non-polar solvents. Free sterols in plants are mono hydroxy  secondary alcohols varying in the constitution of methyl or ethyl group and in double bonds. Cholesterol, ergosterol, bile acids, sex harmones, cortical harmones and D-vitamins are some of the sterol derivatives.
   
The most widely distributed sterols in higher plants are the C₂₉ sterols, stigmasterol, β sitosterol and g sitosterol. Together, they are called steroids. In plants, steroids are present as glycosides. Cholesterol was first isolated from gall stones. The word cholesterol means “solid alcohol from bile”. Bile is a fluid which is formed and stored in the gall bladder. It aids in the digestion of fats in the digestive tract. Sterols form an important group among the steroids. Unsaturated steroids with most of the skeleton of cholestane containing a 3β-hydroxyl group and an aliphatic side chain of 8 or more carbon atoms attached to position 17 form the group of sterols.

Alkaloids are naturally occurring substances of plant origin that are alkaline in reaction. They are optically active, contain nitrogen as part of a heterocyclic ring and have varying degrees of psychological responses in man and animals. They exhibit significant pharmacological activity. Alkaloids are mostly basic in character but also includes some related compounds with neutral and even weakly acidic properties. Presently more than 2000 alkaloids are known.

Nomenclature of alkaloids
Alkaloids are named by adding the suffix ‘ine’ to various distinguishable features such as sources, physiological action in animals, their properties etc. Thus, 1. The name of the genera of the plants containing them Ex. Atropine (Atropa), nicotine (Nicotiana) etc. 2. The names of the species of the plants producing them Ex. Cocaine (Erythroxolon coca). 3. The common name of the organism producing them Ex. Ergotamine (ergot fungus). 4. The specific physiological activity observed in animals Ex. Emetine (emetic – causing vomiting), narcotine (narcotic-alters mind).

Vitamins are essential organic nutrients required by an organism in small quantities. They are essential since they cannot be synthesized in sufficient quantities by an organism, and must be obtained from the diet. The term vitamin does not include other essential nutrients like dietary minerals, essential fatty acids, or essential amino acids which are needed in larger quantities than vitamins. The term vitamin is derived from “vitamine,” coined in 1912 by the Polish biochemist Funk. The name is from vital and amine, meaning amine of life. Thirteen vitamins are generally recognized presently. Vitamins are used in small quantities by an organism to keep it in a healthy condition.

Vitamins are given letters of alphabet (A,B, C, D, etc.) in the order in which they have been discovered. The letters do not bear any relation to their structures or their importance. Vitamins are classified by their biological and chemical activity and not by their structure. Thus, each “vitamin” refers to a number of vitamer compounds that all show the biological activity associated with a particular vitamin. Vitamin A includes the compounds retinal, retinol, and four known carotenoids. Vitamers by definition are convertible to the active form of the vitamin in the body, and are sometimes inter-convertible to one another, as well.

Plant hormones (phytohormones) are chemicals that regulate plant growth and are termed as ‘plant growth substances’. Plant hormones are signal molecules produced within the plant, and are present in extremely low concentrations. Hormones regulate cellular processes in targeted cells. Hormones also determine the formation of flowers, stems, leaves, the shedding of leaves, and the development and ripening of fruit. Every cell is capable of producing hormones in plants. They shape the plant, affecting seed growth, time of flowering, the sex of flowers, senescence of leaves, and fruits. Plant hormones affect gene expression and transcription levels, cellular division, and growth.They direct which tissues grow upward and which grow downwards. Hormones are vital to plant growth. Hence, they are also known as growth factors or growth regulators.

Before the invention of N fertilizer, mineralization of organic N ( present in dead plants and animals or manure) was the main method used to obtain ammonium and nitrate nitrogen. Mineralization is conducted by soil microbes resulting in ammonium ion. Most Nitrogen is absorbed by plants in the form of Nitrate (NO-₃). Ammonium is converted to Nitrate with the help of  soil bacteria. Nitrogen is a very mobile element and is easily lost from the soil. Ammonium (NH₄+) can be converted to Ammonia (NH₃). The gaseous ammonia can be lost to the atmosphere.  Nitrate can be lost through denitrification. Nitrate nitrogen is reduced to nitrous oxide (N₂O), elemental nitrogen (N₂) and nitric oxide (NO). All are volatile. Denitrification occurs under conditions of high water availability and temperatures higher than 10^ºC.

The word cell was coined by Robert Hooke with the help of compound microscope. Cell is the basic unit of life. A plant cell has three distinct components a). Cell wall b). Protoplasm and c). Vacuole. Cell wall  and  vacuole are considered as non-living substances. The protoplasm which is living is  composed of 1. Cytoplasm and 2. Nucleus. The cytoplasm contains several organelles such as mitochondria, chloroplast, ribosomes, endoplasmic reticulum, golgi complex, lysosomes, plastids etc.

Some cyanobacteria (Blue-green algae) like Nostoc are known to work as one of the two coponenents (photosynthetic partner or phycoboint) of lichens. The second partner of lichen is a lichen fungus or mycobiont. The lichens have not been described as a therapeutic agent in Charak Samhita, the monumental work on materia medica prepared by great Acharya Charak and his group in India more than three thousand years ago. However, these have been used in folklore medicine in many countries of the world and have been regarded to bear antibacterial properties (Katiyar and Katiyar, 2009). They are found in tundra even, the treeless plain of the arctic regions of Russia, Siberia, marshy in summer and frozen hard in winter. It is common to see gunny bags full of lichens for sale in the Masala market near Kesa House, Express Canal Road, at Kanpur. They also comprise a part of meat masala (spices for meat). There are references that Raja (King) Todarmal studied algae in relation to purification of water.

However, the search for antibiotics began in the late 1800s, with the growing acceptance of the germ theory of diseases; a theory which linked bacteria and other microbes to the causation of a variety of ailments. As a result, scientists began to devote time searching for drugs that would kill these disease-causing bacteria.

Cyanobacteria have been identified as one of the most promising groups of organisms from which novel and biochemically active natural products are isolated. Cyanobacteria such as Microcystis, Anabaena, Nostoc and Oscillatoria produce a great variety of secondary metabolites. The only group comparable to cyanobacteria is actinomycetes, which has yielded a tremendous number of secondary metabolites. The rate of discovery from traditional microbial drug producer like actinomycetes, which is in the focus of pharmaceutical research for decades, is decreasing and it is the time to turn to cyanobacteria and exploit their potential. Recently, there has been an increasing interest in cyanobacteria as a potential source of new drugs (Glombitza and Koch, 1989; Schwartz et al., 1990). This is of paramount importance to fight increasingly resistant pathogens and newly emergent diseases (Hayashi et al., 1994).

Cyanobacteria are photosynthetic microorganisms that have flourished on earth for more than 3 billion years with some genera showing only minor morphological changes since that time (Hagmann and Juttner, 1996). The medicinal and nutrient qualities of cyanobacteria were first appreciated as early as 1500 BC, when Nostoc species were used to treat gout, fistula and several forms of cancer (Pietra, 1990; Bladon, 2002; Liu and Chen, 2003).

Cyanobacterial species

Cyanobacterial species viz., Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were obtained from National Center for Conservation and Utilization of Blue-green Algae, Indian Agricultural Research Institute, New Delhi. Cultures were maintained in BG-11 growth medium throughout the study in the laboratory.

Culture Media and Reagents

The media used throughout the study were procured from Hi- Media Laboratories, Mumbai, India and the chemicals used were procured locally. Composition of each medium is as follows:

Nine cyanobacterial strains viz., Anabaena variabilis, A. fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were cultured in the laboratory. The growth curve for each culture was studied by growing them in Erlenmeyer flask using BG-11 growth medium. They were examined to determine the early stationary phases of their life-cycle, which are believed to be the most appropriate period for the production of secondary metabolites by an organism. After 23-25 days of incubation period, each culture was harvested and spent medium was collected by filtration of the biomass. Hexane, ethyl acetate, dichloromethane and methanol were used as the solvents for obtaining the extracts from biomass and spent medium of each culture which were then investigated against five pathogenic bacteria viz., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Antibacterial activity of different cyanobacterial sp. has been shown in various tables.

In the present investigation, nine cyanobacterial species viz. Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, Nostoc punctiforme, Nostoc linckia, Nostoc commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. were tested for their activity pattern against some selected bacterial species.
 
Each cyanobacterial species was initially subjected to determine the early stationary phase of their life-cycle, which is believed to be the most appropriate period for the production of secondary metabolites by an organism. The growth curve for each cyanobacterial species was prepared by culturing them in BG-11 growth medium. Optical densities (OD) recorded at 650 nm were plotted against number of incubation days for each cyanobacterium. After 23-25 days of incubation period, each culture was harvested to obtain the biomass and spent medium, which were then subjected to process of extraction by using various solvents viz., Hexane, ethyl acetate, dichloromethane and methanol. The extracts and spent medium thus obtained were then investigated for antibacterial activity against five pathogenic bacteria including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnumoniae and Salmonella typhi.

Present work was aimed to determine the antibacterial activity and minimum inhibitory concentration (MIC) of active crude extracts of selected cyanobacterial species. Nine cyanobacterial strains viz. Anabaena variabilis, A. fertilissima, Nostoc muscorum, N. punctiforme, N. linckia, N. commune, Spirulina platensis, Westiellopsis prolifica and Hapalosiphon sp. belonging to family; Nostocaceae, Oscillatoriaceae and Stigonemataceae were selected from the herbarium of Indian Agricultural Research Institute, New Delhi, and maintained in BG-11 growth medium throughout the study in the laboratory.

The objective of the present investigation was to evaluate the antibacterial properties of cyanobacterial species viz., Anabaena variabilis, Anabaena fertilissima, Nostoc muscorum, Nostoc punctiforme, Nostoc linckia, Nostoc commune, Spirulina platensis., Westiellopsis prolifica and Hapalosiphon sp. against some pathogenic species including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnumoniae and Salmonella typhi. The following are the major findings of the study.

    •    Extracts prepared in methanol were found more active as compared to ethyl acetate, hexane and dichloromethane.
 
    •    Antibacterial activity of extracts varied with the test organisms. Maximum antibacterial activity was shown against Staphylococcus aureus followed by Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Klebsiella pneumoniae was found to be least susceptible against all tested cyanobacterial extracts.

    •    Antibacterial activity was observed against both Gram-positive and a spectrum of Gram-negative bacteria by almost all the cyanobacterial species examined in the present study except Westiellopsis prolifica where activity was observed only against S. aureus, a Gram-positive bacterium.

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Abstract
 
The definition of quality is described from a very simpler one “fitness for purpose or use” to a comprehensive “the totality of features and characteristics of a product or service that bear on its ability to satisfy stated or implied needs”. The concept of total quality management in food industry is adopted recently and it is the “integrated organization approach in delighting customer (both external and internal) by meeting their expectations on a continuous basis through everyone involved with the organization working on continuous improvement in all products/processes along with proper problem solving methodology”. TQM is concerned with the integration of all the efforts in organization towards quality improvement, quality development and quality maintenance to meet full customer satisfaction at economic levels. TQM enhances the work culture and employee’s satisfaction through participation and involvement and consequently the image of the organization.

Abstract

Wheat is unique among cereals because of its ability to form viscoelastic dough. Variety of products such as leavened and unleavened breads, pasta, cookies, and breakfast cereals are prepared using wheat. The viscoelastic property of dough is mainly due to the presence of gluten proteins viz., gliadins and glutenins. Variations among these proteins play a significant role in determining the quality of wheat. Although considerable studies have been carried out on quantity and quality of protein and their relation to bread quality, very little work has been reported on their relation with quality of Indian traditional products like chapati and tandoori roti. Results indicate that ratio between gliadins and glutenins are important in determining the chapati quality. It has been found that varieties having 5+10 HMW glutenin subunits with IBL/ IRS chromosomes are more suitable for the preparation of chapati and roti. This article is focused on the relation between protein and its subunit composition and the quality of chapatti and roti quality.

Abstract

There are enormous food processing industries comprising of grains and oilseeds milling, fruits and vegetables processing, spice grinding and other food processing units in India. Also, there are many seed processing companies in the country, which are engaged in the processing and production of good quality seeds of different crops besides National Seeds Corporation and State Seed Corporations. All industries are using different type of machinery and equipment like cleaner, grader, expeller, milling machines, polishers, dehusking machines, size reduction equipment, different type of grinders, separators, treatment machinery, mixers, pulverizes etc. Besides this equipment, material-handling equipment like conveyor (belt, chain or screw type), elevators, holding bins and other handling equipment are also frequently used in the processing plants. To meet the above requirement many firms are also involved in the manufacturing of these equipment and machinery. Since, most of the seed processing equipment, dhal milling plants, material handling equipment are manufactured by the unorganized sector and hence equipment and machinery fabricated by them are not of very good quality. However, Bureau of Indian Standards has formulated the codes of manufacturing for these equipment and machinery. Seed processing equipment manufactured in India are generally copy of imported processing equipment. The manufacturers do not use materials of good quality. Framework of the machines is very poor. These machines are not also tested for their efficiency, 

Abstract

Mycotoxins are naturally occurring chemicals produced by fungi growing on grain, feed or food. The fungi that produce mycotoxins in grains commonly include Fusarium, Aspergillus and Penicillium species. The toxins can accumulate in maturing corns, cereals, soybeans, sorghum, peanuts and other food and feed crops in the field and in grain during transportation. The toxins may occur in storage under conditions favorable for the growth of the toxin producing fungi. Diseases in animals and human beings resulting from the consumption of mycotoxins are called Mycotoxicoses. These fungal metabolites are detrimental to the health of both animals and human. Toxicity ranges from acute death to chronic diseases, cancer and reproductive malfunction. Mycotoxin contamination of food and feed grains is a serious economic problem for grain producers and processors because grain contamination impacts market 

Abstract

Majority of basic research in our country has foundations in Universities and various Institutions linked to them. This paves way for discovering wilder varieties of the present known cereals and other food crops. Such findings help in bioprospecting the required gene/genetic material for the improvement of the existing crops. As per the Biodiversity Act of 2002, it is the responsibility of these institutions to document the same. But, as research scientists are geared to meet the challenges? Being used to socialistic system from the days of Independence where knowledge generated was open to everyone or closed and kept under the mountains of files, the TRIPS agreement has come as a shock. But the true impact of it was realized in 1999-2000, when the patent amendments were taken up. The private institution/ companies went into the act immediately.  We in the University neither were slow to react nor were we prepared to take the shock.  In this paper some of the problems pertaining to IPR and scenario of Indian Universities and Institutions in this regard were highlighted.

Abstract

Adulteration refers to a process by which the quality or the nature of the given substance is reduced by the addition of foreign or an inferior substance, removal of vital component, packing and storage under harmful conditions, insect infestation, poisonous material, undesirable chemicals and unintentional contaminants. All these change in food whether intentional or unintentional are covered under food adulteration. In cereal grains adulteration results by incidental contamination during the period of growth, harvesting, processing, transport, and distribution and results in various health hazard, sand, marble chips, earth and filth are also adulterants in grains. Pest and pesticides residues are also hazardous.

Abstract

Determination of quality of wheat is very important because quality requirement of an individual product vary from one another. Flour quality can be defined as the ability of the flour to produce a uniformly good end product. Flour quality factors can be divided into two basic groups: those that are inherent in the wheat as a result of the genetic make up and the changes brought about by growing conditions; secondly those factors that might be altered during the process of converting wheat into flour Wheat flour constituents play an important role in determining the quality of flour. The flour quality for baking could be assessed by measuring the physical, chemical and rheological characteristics.

Abstract

Wheat gluten consists mainly of gliadin and glutenin polypeptides. During mixing gliadin and glutenin proteins interact to form a unique viscoelastic gluten network, which is envisaged as being necessary for holding the gases and for producing a light porous crumb textured bread. Recent work has confirmed that the elastic properties of gluten are due to the glutenin fraction, whilst the viscous properties are attributed to the gliadin fraction. Equal amount of these two major protein components of wheat gluten is required for achieving the desired bread quality, but variation in the composition and physical properties of the glutenin polypeptides appear to be largely responsible for the differences in the gluten viscoelasticity and breadmaking potential among wheat cultivars. Recently, associations of gliadin polypeptides with breadmaking quality have also been reported. Using improved protein separation and purification techniques, physical methods and genetic engineering, we are beginning to understand the structure-functional relationship of wheat gluten proteins, but much remains to be explored in the years to come. 

Abstract

Consumers are now more conscious about quality and source of their foods. Attempts made to determine the quality of food materials are numerous, but most of them are destructive in nature. In recent years, nondestructive methods of quality evaluation have gained momentum and a considerable attempts have been made to develop them. This paper describes application of these non-emerging technologies: magnetic resonance imaging, x-ray computer tomography, near infrared spectroscopy and a few other important techniques for determination of different quality attributes with special emphasis on grain, and discusses their pros and cons for practical exploitation.

Abstract

Pulses being nutritionally vital, is an important constituent of diet for a very large number of peoples. A part from being a relatively cheaper source of dietary protein, it is also an important source of calories, certain essential amino acid, minerals and vitamins. Post harvest scenario of pulse shows that 75 per cent of production was milled in pulses with as dehusked split i.e. dal. Traditionally green gram and black gram dal is dehulled manually after soaking in water. Present investigation was carried out to develop the wet type dehuller and study was carried out on water absorption characteristics of green gram and black gram. The result reveals that the dehulling efficiency of abrasion type mechanism was about 95 per cent and 92 per cent in case of shearing type mechanism. It has been observed that rate of water absorption was initially high and then gradually decreases i.e. 0.54 to 0.18 and 0.48 to 0.18 for black and green gram, respectively. 

Abstract

The constant improvement in automation of bakery industry has placed higher demands on wheat. As a consequence bakery industry requires wheat possessing specific quality attributes. In view of this, there is need to produce good quality wheat not only for good chapattis but also for better bakery products viz., bread, biscuit, noodles, pasta products. New technologies are emerging which could be exploited for determining the Indian wheats for their suitability for various value added products. In the current investigation, 50 genotypes possessing low protein content (<10.5%) selected from a vast amount of breeding material were analysed for their biscuit making attributes. These lines were screened by actual baking test and alkaline water retention capacity (AWRC) test keeping the standard prescribed for biscuit making qualities viz., low protein content, low sedimentation value, low AWRC and larger diameter with larger spread factor of biscuit. Further with the help of Stable Micro Systems TA.HDi Texture Analyser the biscuits were analysed for hardness and crispness. Correlation coefficient among 8 quality traits suggested highly significant and positive correlation between sedimentation value and AWRC, biscuit diameter and spread factor, and biscuit hardness and fracturability, and significant negative correlation between biscuit diameter and its thickness. On the basis of findings, genotypes identified for biscuit making qualities may be used as donor for developing varieties suitable for biscuit making.

Abstract

The control of micronutrient deficiency is a realizable goal, notwithstanding the magnitude of the task, challenges and constraints that need to be resolved. Since wheat products particularly maida and atta form the major raw material in widely consumed food items like chapati, bread and biscuit. Fortification of flour as followed in many other countries could be an important strategy to help realize this goal. The technology of flour fortification is effective and cost affordable. Effective flour fortification program would require collaboration between scientific community, governmental and non-governmental agencies, and industry and consumer groups.

Abstract

30 lines were selected from 864 germplasm lines for good biscuit making attributes on the basis of low protein content (<10.5%) and low sedimentation value (<40cc). These lines were further screened by actual baking test and alkaline water retention capacity (AWRC) test keeping the standard prescribed for biscuit making qualities viz., low protein content, low sedimentation value, low AWRC and larger diameter with larger spread ratio of biscuit. AWRC had significant negative correlation with biscuit spread ratio. Positive significant correlation was found between biscuit spread ratio and biscuit diameter. On the basis of above findings, it may be recommended that low protein content, low sedimentation value, low AWRC may be used as criteria for better biscuit making i.e. increase biscuit spread ratio and biscuit diameter. So wheat-breeding programme has opened up new opportunities for the end use quality product and wheat based food industry. Hence there is an urgent need for qualitative analysis of desired traits in wheat towards enhanced value addition and thereby an attempted has been made to identify suitable genotypes possessing good biscuit making attributes

Abstract

Paddy seeds of variety PR - 116 were obtained from the PAU seed farm. These seeds were cleaned with commonly used seed processing equipment. Samples were divided into 2 parts. One part was kept as such for taking the observations of feed (control) and the remaining part was passed through specific gravity separator for its refinement. The various fractions obtained were recovery, middlings, and rejections. These graded fractions as well as the feed were packed in 5-kg cloth bags and stored under ambient conditions. Different quality parameters viz. moisture content, physical purity, thousand grain weight, percent germination, root/shoot length, vigour index and speed of germination were recorded at 30 days interval. The physical purity, thousand grain weight, percent germination, root length, shoot length and vigour index were found to be higher in recovery and minimum for rejections while moisture content was highest for rejection as compared to all other fractions. Germination, root/ shoot length, vigour index and speed of germination increased with the duration of storage while moisture content, thousand-grain weight and physical purity followed a reversed trend. Compared to feed, the recovery had higher quality attributes after 4 months of storage viz. moisture content decreased by 0.3%; physical purity increased by 0.67%; thousand grain weight increased by 2.97 gm/ thousand grain, germination increased by 8%; vigour index increased by 481.1 points; speed of germination increased by 3.2 seedlings per day. The grading resulted in improvement in overall quality of sunflower seed.

Abstract

Garden peas of two cultivars (Pb-87 and Pb-88) were procured. Pods were shelled manually, grains were blanched and dehydrated. Other lot of peas of both the varieties was allowed to mature on the plant till the plant foliage dried up, harvested, pods were dried in shade for few days, shelled manually and grains were further dried in shade. Grits were prepared by grinding rice and pea (dhal) in a laboratory grinder to pass through 20 mesh sieve. Blends were prepared by mixing rice and pea grits in the ratio of 100:0, 85:15 and 70:30. The blends were subjected to extrusion cooking using Brabender single screw extruder. The extruder die pressure, specific energy consumption (SEC), expansion ratio, density, water absorption index (WAI), water solubility index (WSI) and organoleptic characteristics were studied. Addition of pea grits resulted in a decrease in die pressure in all the blends. Addition of pea grits showed a significant effect on SEC. A decrease in expansion with increase in the level of pea grits in blend was observed. The extrudates having lower expansion showed higher density values and vice versa. Effect of replacement of rice grits with pea grits on WAI was found to be significant. Level of pea grits significantly affected the WSI. Incorporation of pea grits in the rice grits significantly affected the sensory characteristics. Replacement of rice grits with mechanically dried pea grits resulted in deterioration of acceptability score, but addition of field dried pea grits improved the same.

Abstract

India has become not only self sufficient in food grains but has also emerged as net exporter of wheat and rice. Acute famines in India have practically been eliminated, thanks to increased food availability at the national level, improved early warning systems and expanding infrastructure for more rapid transport of food grains and other products to needy areas. However, the existing post management system, strategies and policies don’t seem to match new challenges. Despite government intervention in pricing and procurement of food grains, primarily wheat and paddy, India continues to suffer heavily on account of losses and wastages incurred during the post harvest stages, which are more manmade than natural. Some losses have already occurred before the arrival of food grains in the regulated and wholesale markets and continue till food grains reach their destination. What contribute to continuing losses and wastages are improper post harvest handling, government policies and their implementation, which even stifle growth and modernisation of grain markets and processors.

Abstract

‘Made in India’ is no more a sign of poor quality. However, in the field of food grain exports, India is still lacking the required determination. The Indian food grains get rejected in the International market because of its out-dated post harvest technology (PHT). The Food Corporation of India (FCI) is the nodal national agency for the procurement, storage, processing and transporting of food grains, and their further distribution through the Public Distribution System (PDS). The government fixes the procurement price as well as the distribution price of the food grains, and the FCI has to accept it. The government compensates the resulting loss through subsidy. The government, through FCI, must encourage cultivation and marketing of premium quality food grains, by offering suitable incentives to farmers. The cost of marketing, storage and transporting of food grains can be considerably reduced with simultaneous improvement in quality of the end product, if the prevailing bag handling is replaced by bulk handling, storage, and transportation. However this needs closer coordination between various departments of the Ministry of Food and Ministry of Railways. The President of India revealed his vision to make India a developed country by the year 2020. To achieve this, the current post harvest losses to the tune of 10 percent must be reduced to 2 percent. This is possible if the government takes bold political decision supported by equally firm and time-bound necessary

Abstract

High moisture paddy can be dried in a trolley drier at farm level/grain market to reduce losses during various post harvest operations. During last two paddy harvesting seasons quality analysis of eight, most popular varieties of fresh paddy arrivals from about 40 grain markets, scattered all over Punjab, was performed. Monitoring of paddy in grain markets showed that maximum number of samples (62.7%) was in the range of 12-18%, moisture content (w.b.). Over-dried paddy samples (<12% m.c., w.b.) were only 9.1%. The samples with more than 18% m.c ,w.b. (high moisture paddy) were 28.2%, which have to be dried to safe moisture levels. Milling characteristics of the analyzed samples indicated that the broken percentage was highest (25.5%) in PR-113 followed by PR-111 (20.6%) and minimum in Pusa-44 (12%). The highest insect- pest infestation (Sitotroga cerealella (olivier) was observed in PR-108, followed by PR-116, PR-113, PR-111, Lal Mundi, Govinda, PR-114 and Sharbati. The Mycoflora isolated and identified were Aspergillus, Penicillium, Mucor, Rhizopus, Alternaria, Curvularia, Helminthosporium and Cladosporium spp. Maximum fungal infestation were associated with samples of PR-108 variety. To avoid further proliferation of mycoflora and inset pests, paddy should be dried to safe moisture levels for further storage and processing. For this, a trolley drier has been developed by converting a conventional farmer’s tractor trolley, into a portable drier, which can reduce 5% moisture of 1 tonne batch of paddy in about 100 minutes, consuming 56 kWh of electrical energy

Abstract

Sunflower seeds of variety PSFH- 67 were obtained from the PAU seed farm. These seeds were cleaned with commonly used seed processing equipment. Samples were divided into 2 parts. One part was kept as such for taking the observations of feed (control) and the remaining part was passed through specific gravity separator for its refinement. The various fractions obtained were recovery (heavier, healthy seeds), middlings (intermediate, under size) and rejections (lightweight seeds, wevil eaten, foreign matter). The respective percentages of each fraction were computed. These graded fractions as well as the feed were packed in 2-kg cloth bags and stored under ambient conditions. Different quality parameters viz. moisture content, physical purity, percent germination, thousand grain weight and vigour index were recorded at 30 days interval from Jan, 2010 to Apr. 2010. The physical purity, moisture content, thousand grain weight, percent germination, root length, shoot length and vigour index were found to be higher in recovery and minimum for rejections while moisture content was highest for rejection compared to all other fractions. The physical purity remained more or less constant throughout the period of storage. The thousand-grain weight showed a rapid decrease during first month thereafter it remained constant. There was continuous decrease in percent germination with the period of storage irrespective of the treatment.

Abstract

The establishment of All-India Coordinated Wheat Improvement Project in 1965 coincided with the introduction of high yielding semi-dwarf wheat varieties. Since then India has made very impressive progress in increasing wheat production over the past three and half decades. India now ranks as the second largest wheat-producing nation in the world. In fact from a state of “Ship to mouth” in 30 years or so India has become a nation of surpluses. The significant contribution made by the project in helping the increased wheat production cannot be ignored. Through coordinated research and development, nearly 200 wheat varieties have been developed during the last 35 years in India. However, only a small number of them produced without backing of specific quality breeding programs excel in quality traits also. The increasing demand of baked and pasta products in the country along with economic liberalization and global trade have offered opportunities for better utilization of the surplus wheat. Thus, in the current decade and beyond, wheat quality needs uppermost attention to meet the trade requirements for domestic and export purposes. Therefore, there is a need to reorient the wheat improvement programs in the country.

Abstract

Four bread wheat varieties (PBW-138, PBW-299, PBW-343 and PBW-373), two of durum wheat (PDW-215 and PDW-233) and two of triticale (TL-419 and TL-1210) were milled to obtain 70 percent extraction flour.  The grains and the flour samples of all the varieties were evaluated for proximate composition, rheological properties and pretzel making. Physical properties of durum wheat were better followed by bread wheat and triticale. Durum wheat were harder, had higher grain weight than bread wheat and triticale.  Protein, ash, fat, total sugars and enzyme activity were higher in triticale flour than bread and durum wheat flours while durum flour had higher pigments (ppm, b-carotene). Bread wheat flour was intermediate between durum and triticale flours with respect to fineness. Rheological studies revealed triticale flour to be the weakest flour. Pretzels prepared by using optimized recipe and method revealed PBW-343 and PBW-373 best for pretzel making.

Abstract
 
Technological advancement, increased urbanization, awareness and interest of consumer for variety products have given a great challenge to the food sector to develop inexpensive foods that are nutritionally superior and acceptable to the intended consumers. Keeping this objective in view, we have made an attempt to develop technology for variety products from wheat and corn and suitability of available varieties judged. With the emerging trends of food market towards fast foods, there are vast prospects for efficient utilization of cereals by exploring varieties products. A steamed bread (a Chinese traditional product) and Tortilla and corn chips (Mexican snacks) were developed. Steamed bread is defined as the product resulting from cooking or steaming fermented dough. Recipe and method for its preparation was standardized. Nutritionally rich steamed bread can be prepared by supplementing 10% level of chickpea and defatted soybean flour. Corn snacks have become a prime target of some consumer advocates and nutritionists who have referred them as a source of ‘empty calories’. But reality is that these snacks provide instant energy, as they are rich storehouse of nutrients. Process and recipe for the preparation of Tortilla and corn chips from Indian maize were standardized. The quality and acceptability of these snacks were worked out and found that these products were highly acceptable. Indian consumers have versatile taste, so these products will be readily accepted in Indian market.