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In nature there are nearly 300 nuclei, consisting of different elements and their isotopes. Isotopes are nuclei having the same number of protons but different number of neutrons. These techniques are now being used in almost all the areas of plant physiology, soil chemistry and plant biochemistry. In agriculture, radioisotopes are used in the nutritional studies of major and minor elements, plant metabolism, mechanism of photosynthesis studies, and uptake of nutrients and ions mobility in soil. In applying this technique, a minute quantity of radioisotope element is usually mixed with ordinary element of the same kind, the whole batch is termed “tagged” and their role in chemical reactions is traced. The radio labeled fertilizer has been used to study the uptake, retention and utilization of fertilizers.

Radioisotopes are used for determining the function of fertilizer in different plants. The tracer technique is used to study the rate and direction of movement of an element in a plant. For this a radioisotope of that element is injected in the ground near the plant. After a few days the plant is laid on a photographic paper to produce an autoradiograph. The dark areas in the radiograph show the positions reached by the element. This technique gives valuable information regarding the optimum season for fertilizing crops and for poisoning weeds. Use of N¹⁵ in organic fertilizer studies has significantly advanced the understanding of N release from organic materials. Crop residue or green manure studies are relatively simple. These techniques have been extremely useful in determining how residue quality parameters affect mineralization values. Because of the attention focused on nutrient cycling and management studies in recent years, a number of new P isotope techniques have been developed.

Model organisms are widely used in biological researches to describe basic biological processes. When selecting living organisms as models to work with, a wide range of characteristics are used which include: 1) rapid development with short life cycles, 2) small adult size, 3) ready availability and 4) tractability. Being small, growing rapidly and being readily available are crucial in terms of housing them, given the budget and space limitations of research and teaching laboratories. Tractability relates to the ease with which they can be manipulated. Many model organisms have been used including fruit fly, Drosophila melanogaster, the most obvious organism used for teaching Mendelian genetics and developmental biology; Escherichia coli, a favoured organism for molecular biological studies involving recombinant DNA technology. Research into the molecular and developmental biology of C. elegans was begun in 1974 by Sydney Brenner and it has since been used extensively as a model organism.

C. elegans is a popular research organism as it possesses all the characteristics mentioned, yet shares many essential biological properties with humans. For instance, researchers who study apoptosis (programmed cell death) use C. elegans as an experimental organism in the hope of finding treatments for certain types of human cancers, such as leukaemia.

C. elegans is easy and inexpensive to maintain in laboratory conditions with a diet of E. coli. Embryogenesis occurs in about 12 hours, development to the adult stage occurs in 2.5 days and the life span is 2-3 weeks. The short, hermaphroditic life cycle (~3 days) and large number (300+) of offspring of C. elegans allows large-scale production of animals within a short period of time. Since C. elegans has a small body size, in vivo assays can be conducted in a 96 well microplate. Laboratory stocks of C. elegans can even be stored in liquid nitrogen for 25 years without losing their viability. The transparent body allows clear observation of all cells in mature and developing animals. Furthermore, the intensively studied genome, complete cell lineage map, knockout (KO) mutant libraries and established genetic methodologies including mutagenesis, transgenesis and RNA interference (RNAi) provide a variety of options to manipulate and study C. elegans at the molecular level and for a more detailed presentation of genetic and genomic resources.

Oilseed Crops consist of those groups of plants cultivated for substantial quantities of the oil, vegetable fat obtained from the seeds (endosperm) of the plants.

The major oilseeds grown world over are soybeans, sunflowers, rapeseed – mustard (or canola in USA & Canada), groundnuts (peanuts) and linseed. Seed oils from flax (linseed) and castor beans are used for industrial purposes. These Oilseed crops including oilseed bearing trees are the basic biological systems that produce oils, contributing to renewable energy production, help stabilize greenhouse gases, and mitigate the risk of climate change (Jaradat, 2016)

The oilseed crops are of heterogeneous group of plant species not having any common morphological and genetic features except production of seed oil. Crops like groundnut, sesame, castor bean, soybean and niger are grown during the rainy season and the others like rapeseed and mustard and safflower are grown in the post rainy season. Sunflower, however, may be grown in both the seasons. Hence the individual crops forming the conglomerate of Oilseed crops have to be treated differently for their improvement and production. Nevertheless, all of them produce a common end product viz., oil or vegetable fat, the importance of which is growing in product development, commerce and human and farm animal nutrition. The oilseed bearing crops have to be treated as a unique group in terms of research and development to come up with strategies for their judicious exploitation in the national farm economy. From the scientific point of view, the oilseed crops are energy rich and offer impressive potentials in terms of realizable production levels and valuable product development. In India good progress has been achieved so far in research and development of some oilseed crops; but there exists a need to scale to newer heights in terms of realising the hidden potentials of oilseed crops through basic and strategic research, with an integrated approach.

Leavening agents produce carbon dioxide which helps to give characteristic structure to bread cakes and biscuits etc. In the case of cakes, leavening is produced by air entrapped in the dough due to the presence of egg in the mix. The important groups of leavening agent’s are l. baker’s yeast 2. Chemical leavening agents

Types of Enazymes in milk

1. Endogenous and 2. Exogenous.

Three sources of enzyme in milk:

1. The blood via defective mammary cells.

2. Secretory cell cytoplasm. Some of which are occasionally entrapped with fat globules by the encircling MFGM.

3. The outer layer of MFGM is probably the principal source of endogenous enzymes in milk. The outer layer of MFGM is derived from the apical membrane of the secretory cell which in turn originates from the Golgi membrane and acts as the principal source of enzyme in milk.

General Characteristics of Milk Enzymes

1. Deteriorative character e.g. lipase (commercially most important enzyme in milk), proteinase, acid phosphatase, xanthine oxidase.

2. Preservative enzymes. e.g. superoxide dismutase, sulfhydryl oxidase.

3. As an indicator of the thermal treatment history of milk. Related enzymes are alkaline phosphatase, ϒ glutamyl transpeptidase, and lactoperoxidase.

4. Mastitis infection indicator. Related enzymes are catalase, n-Acetyl-β D-glucosaminidase, and acid phosphatase.

5. Antimicrobial activity.

6. Commercial source of ribonuclease and lactoperoxidase

Common Milk Enzymes and their Role in Dairy Industry

Introduction

This led to an extended debate and international co-operation in the recognition of plant-related intellectual property rights, resulting in a greater attention being paid to questions of ownership of genetic resources. At the same time concerns for sustainable use of biological diversity were also raised.

One of the major events was the Convention on Biological Diversity (CBD) which came into force in 1993, which was adopted during the Rio Earth Summit of the United Nations. It was the first legally binding institutional mechanism, providing for conservation and sustainable use of all biological diversity and intends to establish the process of the equitable sharing of benefits arising out of the use of biodiversity.

The Convention on Biological Diversity

The CBD was conceived in the 1980s and was negotiated under the leadership of the United Nations Environment Programme (UNEP). It was adapted in June 1992 at Rio de Janeiro, at the Earth Summit, and is effective since December 1993.

Abstract

Genetic analysis of yield and yield components in tomato (Lycopersicon esculentum Mill.) was worked out by using half diallel mating design. The good general combiners observed were Solan Vajr, FT-5 and UHF-265 for plant height; UHF-107 and UHF-260 for days to first picking; Solan Vajr for number of fruits per cluster; FT-5, EC-191536 and UHF-120 for average fruit weight; Solan Vajr, UHF-265 and FT-5 for harvest duration and FT-5 and Solan Vajr for marketable yield per plant. The best three specific combiners for marketable yield per plant were EC-191536 x Solan Vajr, BL-342-1 x FT-5 and UHF-107 x EC-191536. Hayman’s approach revealed non-allelic interaction in the expression of number of fruits per cluster, number of fruits per plant and marketable yield per plant. Preponderance of non-additive gene action was observed with dominance towards tallness, delayed first picking, more fruits per cluster, longer harvest duration and higher marketable yield, while involvement of complimentary epistasis was indicated in the inheritance of average fruit weight and number of fruits per plant.

Amino acids serve as the building blocks of proteins and as precursors of many other important biomolecules such as hormones, purines, pyrimidines, porphyrins and some vitamins. They also serve as sources of energy when present in excess amounts and when they are used as fuel, amino acids undergo deamination reactions. The remaining carbon skeleton will either convert itself into glucose or undergoes oxidation to CO₂ via TCA cycle.

Central dogma: The Central dogma of molecular biology, which has been formulated as a simple linear progression of information from DNA to RNA to Protein, is summarized in the illustration. The replication process on the left consists of   passing  information from a parent DNA molecule to daughter molecules. The middle  transcription process copies this information to a mRNA molecule.  Finally, this information is used by the chemical machinery of the ribosome to make polypeptides.

Sweet gourd, (Momordica cochinchinensis) (Cucurbitaceae),  called as ‘spiny gourd’,  ‘crow cucumber’, ‘spike fruited crow cucumber’ or ‘elephant bitter gourd’ and ‘gac fruit’ is a wild vegetable, native to East and South East Asia comprising India, Myanmar, Philippines, Vietnam, Thailand, Japan, China, Indonesia and New Guinea (de Wilde and Duyfjes, 2002). It is by far the most extensively distributed and more variable species among the genus Momordica L. Even though, it is restricted to Andaman Islands and Assam in India, it occurs as a natural component of coastal scrub jungles and partially opened forests especially stream banks in South, Middle and North Andaman Islands (Joseph 2005). It is cultivated in Bihar, Jharkhand, Orissa, West Bengal and Assam by tribal people in their homegardens. It is grown as a vegetable in China and Japan (Jeffrey 2001). It is known by various vernacular names as ‘Adavi kakrol’ (Telugu), ‘Hathiya kankad’, ‘Gol kakra’ (Bengali) and ‘Jangli Kakrol’ (Andaman Islands).

Answer the followings
    Q.1.    What is the international organizations associated for making outline the mastitis control programme?
    Ans.    National Institute for Research on Dairying (NIRD) and National Mastitis Council (NMC).

    Q.2.    What is the important objective of dairy industry as regards to mastitis control?
    Ans.    The important objective is to achieve an excellent udder health for production of high quality of milk.

    Q.3.    What is the basic principles of mastitis control programme?
    Ans.    There three basic principles of mastitis control programme:
    a)    Elimination of existing infection
    b)    Prevention of new infection 
    c)    Monitoring of udder health status

2.1 Introduction

Automation of agricultural field with IoT and microcontroller is new era of technology. Arduino based solution to agricultural field is open source platform. It can be used for developing data loggers and new sensors environment. The rate of solutions with Arduino and sensors is high as it is open source platform. The use of Arduino in agriculture can accelerate the growth of automation. Arduino can be interfaced with sensors like:

• Temperature
• Humidity
• Soil Moisture
• Water Pressure
• Water Flow and Water Metering
• Rain Gauge

Towards the end of the third week of embryological development, the notochord induces the overlying columnar ectodermal cells of the embryonic disc to become pseudostratified neuroepithelial cells and form a spoon shaped thickening of dorsal ectoderm called the neural plate. The cranially expanded region of the neural plate forms the primordium of the brain, while the narrower region caudal to the brain gives rise to spinal cord.

The raised lateral edges of the neural plate form the neural folds while the depressed midline region of the plate forms the neural groove. Cellular proliferation at the medial aspects of the neural folds causes these structures to gradually approach each other in the midline, meet and fuse, forming the neural tube which encloses a central neural canal.

Chemistry of Creaming

After being drawn from animals, normal milk will form a cream layer on standing. Because of the difference in density between fat globules (920 kg m−3 at room temperature) and milk plasma (density 1030 kg m−3), the granules tend to rise. This causes creaming, which we often want to prevent and it enables milk to be separated into cream and skim milk. Creaming is much enhanced when the globules have been aggregated into flocculates, clusters, or granules.

Cream Ripening

This unit operation serves as an intensive preparation of the cream for the churning process. It is divided into two categories.

1. Physical ripening;

2. biochemical ripening The following criteria are influenced by the cream ripening:

• Consistency, firmness, and spreadability of butter.

• Basic water content of butter during the manufacturing process (bound water in butter, which cannot be removed by any mechanical treatment).

• Buttermilk fat content, which has to be minimized.

The melting and solidification behavior of fat depends on the attached fatty acid, which indicate the hardness of the butterfat.

Natural hardness is caused mainly by seasonal feeding of the milk animals. Winter feed with a great deal of dry feed or roughage yields a hard butterfat with a high percentage of saturated and short-chain fatty acids and few double bonds in the fat molecules.

Summer feeding (green grass) results in fat with longer chains and a higher percentage of unsaturated fatty acids with many double bonds. We therefore distinguish between summer fat and winter fat or summer cream and winter cream.

Intellectual property (IP) refers to creations of the human mind, such as inventions, literary and artistic works, symbols, names, images, and designs, which are protected by law. The protection of intellectual property is essential for promoting innovation, creativity, and economic growth. In order to ensure effective protection of intellectual property rights, various governing bodies, organizations, agreements, and treaties have been established at national and international levels. These bodies are responsible for setting and enforcing standards and regulations related to IP, as well as facilitating cooperation and coordination among different countries and stakeholders. Some of the major international organizations and agreements that deal with intellectual property include the World Intellectual Property Organization (WIPO), The Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS), and the Paris Convention for the Protection of Industrial Property. These organizations and agreements play a crucial role in creating a conducive environment for innovation, investment, and development, while at the same time ensuring fair and equitable access to intellectual property for all.

Herbal medicines have become popular these days as complementary therapies for various disorders. These are available as single compounds, enriched extracts, or polyherbal preparations. The polyherbal formulations behave very differently as compared to the single compounds due to the interaction of different chemical constituents, present therein, with multiple receptors/targets in the biological system. The presence of the multiple chemical constituents is also likely to exhibit pharmacokinetic and metabolic interactions. Thus, it becomes difficult to monitor and predict the exact pharmacokinetic profile of all the chemical constituentspresent in a polyherbal formulation (He et al. 2011; Xie et al. 2012). As a result, pharmacokinetic studies of polyherbal formulations have been a long-standing hurdlein phytotherapy research. Conventional scientific approach for studying thepharmacological behaviour of a multicomponent therapy is to isolate the individualcompound and study the effect of these compounds on various targets and enzymes, independently. To rationalize the use of herbal medicines,a clear understanding of their pharmacokinetics and metabolism in the physiologicalsystem is required. A fundamental knowledge of pharmacokinetics of phytopharmaceuticals will be helpful inauthenticating their use.

Teasle gourd, Momordica subangulata Blume ssp. renigera (G. Don) de Wilde (Cucurbitaceae), is a semidomesticate or a wild vegetable native to Assam-Myanmar region. As in  other dioecious Momordica species, it’s taxonomy and nomenclature were confused with M. dioica Roxb. (spine gourd=kartoli) and M. cochinchinensis (Lour.) Spreng. (sweet gourd=kakrol) (Joseph et al.2006). It is known by many vernacular names like kaksa (Bihar), lamkarote (Manipur), meeta chotela (Sikkim), kakrol (Bengal, Tripura), kakrul (Mizoram), bhat karela (Assam) and gantola (Bangalore). Allied to M. dioica, M. cochinchinensis and M. sahyadrica Joseph and Antony, in general morphology it is distinct from these species in its branched tuberous roots, cordate dentate unlobed leaves, prominently networked leaf veins, large creamy flowers with bull’s eye mark in three inner petals (also in M. cochinchinensis), reniform male bracts, very long female flower stalks and medium sized softly echinate dome shaped fruits with remnant ridges (Fig.1). A few workers reported it as tetraploid form of M. dioica (Misra and Sahoo 1983). It is referred to as M. cochinchinensis by Patnaik and Patnaik (1976), Shadeque and Baruah (1984) and Ram et al. (2002) and M. dioica by Maurya (1976) and Ali et al. (1991), whereas a recent revision of the genus in Malaysia and SE Asia by De Wilde and Duyfjes (2002) proved its botanical identity as Momordica subangulata ssp. renigera  (G. Don) de Wilde.

Introduction

Cotton is a major commercial crop grown on nearly 12 m. ha area. About 65% of the area is rain dependent. It is grown on a wide range of soil types and climatic conditions. Soils supporting cotton in the rainfed areas are Vertisols (Vertic intergrades), Inceptisols and Alfisols. Rainfall ranges from 500-1200 mm in these regions. Both Alfisols and Vertisols have different potentials and problems but both need organic C improvement and soil moisture conservation measures to restore their productivity. This can only be achieved through resource conservation strategies. Conservation agriculture (CA) is one such strategy that focuses on resource conservation. It refers to a system of raising crops without disturbing the soil while retaining crop residues or soil surface. 

BACTERIA HAVE SMALL, COMPACT GENOMES

We now know the complete DNA sequences for about a dozen bacteria

Bacteria seem to need about 500 to 5000 genes to conduct their lives

Because they are small, bacterial genomes are highly organized

Most of the time there are only a few bases between the end of one gene and the beginning of the next

Few introns are found in bacteria

Bacteria contain accessory “chromosomes” called plasmids; each has only a few genes

Development of sophisticated procedures for the isolation, manipulation and expression of genetic materials is a field called genetic engineering. Genetic engineering has applications in both basic and applied research. In basic research, genetic engineering techniques are used to study the mechanisms of gene replication and expression in prokaryotes, eukaryotes and viruses. For applied research, genetic engineering used for development of microbial cultures capable of producing valuable products such as human insulins, growth hormones, interferons, vaccines, enzymes etc. The commercial application of genetic engineering is sometimes referred as Biotechnology. In broad sense, Biotechnology refers the use of living organisms to carry out defined chemical processes for industrial application.

The process of isolation, purification and replication of specific DNA fragment is called molecular cloning or gene cloning. Molecular cloning is the base of most of the genetic engineering procedures. The purpose of molecular cloning is to isolate large quantity of specific genes in pure form. Thus molecular cloning is very important in genetic engineering and can be divided into several steps depends on the purpose. The steps are as follows:

ABSTRACT

In this study, different concentrations of acetone, alcohol, benzene and chloroform extract of Wedelia chinensis were carried out against human pathogenic bacterial and fungal strains. High frequency of anti-bacterial activity was observed on the Escherichia coli. Moderate amount of antibacterial activity was observed on Staphylococcus aureus. Highly resistant bacterium to this plant was screened through antibacterial pour plate method, which showed on the following organisms Citrobacter diversens, Serratia marcescens and Streptococcus pyogenes. The aim of this study is main evaluation of antimicrobial activity of plant. The ethanolic extracts demonstrating antibacterial and antifungal activity could result in the discovery of antibacterial and antifungal agents.