eChapters

Introduction

Seed is the key input in agriculture deciding the fate of productivity of any crops. Indeed, seed is referred as a nature’s “nano-gift” for agriculture. Seed is a smart delivery system where we shall make manipulation to achieve the desirable goals. The seed technologists in the country were trying to find a second or third generation technologies to address the issues of seed science. Nanotechnology is one of the cutting edge research areas that has enormous potentials to solve the unresolved field problems. The application of nanotechnology to the agricultural and food industries was first addressed by a United States Department of Agriculture roadmap published in September 2000. Nanotechnology in the agricultural front has been more useful in improving the existing crop management techniques. Most of the applications of nanotechnology spearhead around nano encapsulated agrochemicals, herbicides, nano coated gene delivery and nano fertilizers (Nair et al., 2010).

In recent years application of nanotechnology in various fields including agriculture has received increased attention. Nanotechnology offers an important role in improving the existing crop management techniques especially in plant disease management by controlled and targeted release of agrochemicals to enhance their efficiency. The nano-based delivery systems are beneficial because of improvement in efficacy due to their higher surface area, higher solubility, induction of systemic activity, higher mobility and lower toxicity (Sasson et al., 2007). The pharmacokinetic parameters of nano particles may be altered according to size, shape and surface functionalization. They can also be used to alter the kinetic profiles of drug release leading to more sustained release of drug there by reducing the frequent application (Sharon et al., 2010). The successful use of metal nanoparticles in medical streams as antimicrobial agents has also led to their applications in controlling phyto pathogens (Nair et al., 2010).

Fruits and vegetables are perishables and the post-harvest losses from the point of production at the farm gate till it reaches the consumers is estimated as 30-35 per cent which constitute a major economic drain of more than 33 billion USD equivalent to Rs 2,40,000 crores annually. In order to reduce the losses at various stages, nanotechnologies have come as potential strategies to minimize the losses while improving the marketability and profitability of perishables. One of the plants derived natural compounds “hexanal” which is known to extend the shelf-life of fruits when it is used as a pre-harvest spray or post-harvest dip as a nano-emulsion. Since, it is a highly volatile compound, efforts were undertaken to entrap the compound in the polymer-based nano f ibres (as nano stickers) or as a cyclodextrin inclusion complex (as nano pellets) in order to preserve the perishables. Further, hexanal vapour was also evaluated to preserve fruits. In this book chapter, various nanotechnologies tested, evaluated and technologies developed over the years are elucidated. Overall, the literature review suggests that use of any of the nanotechnologies delivered as pre-harvest spray, post-harvest dip, nano-stickers, nano-pellets and vapour singly or in combination found to reduce the infestation of post harvest diseases, minimize the losses and extended shelf-life by 2-3 weeks that help the farmers to get lucrative prices due to the late arrival in the market besides significantly improve the productivity and profitability of orchards.

Biological diagnostic methods rely on the transmission and amplification of the pathogen in indicator hosts that develop symptoms. These methods test the biological properties of pathogens and early successes in the control of intracellular pathogens were largely due to the diagnosis of the causal agents by biological tests. The sensitivity of biological/serological tests is usually determined by the minimum number of pathogen propagules required to initiate infection. Inoculation with fewer particles could lead to false negative results. Biological techniques for disease diagnosis and pathogen detection are usually highly accurate but too slow and not amenable to large-scale application. 

Summary

Nanotechnology, the science of nanoscale structures has been applied in various fields including in biomedical sciences. Nanotechnology is being increasingly used to create materials and devices so as to interact with the body at sub-cellular scales with a high degree of specificity. Nanotechnology is used in the fight against cancer, cardiac and neurodegenerative disorders, infection and other diseases. This chapter provides an overview of some of the applications of nanotechnology in the field of biomedical sciences particularly with a focus on tissue engineering and medical diagnostics.

Nanotechnology: The Next Big Thing is Really Small

Nanotechnology can be considered as a brainchild of Richard Feynman, as the conceptual underpinings of nanotechnologies were first laid out in 1959 in his historical lecture, ‘ There is plenty of room at the bottom’. The term ‘nanotechnology’ was not used until 1974, when a researcher, Norio Taniguchi, at the University of Tokyo, Japan, used it to refer to the ability to engineer materials precisely at the nanometer level. The prefix ‘nano’ is derived from the Greek word ‘drawf’. One nanometer (nm) is equal to one billionth of a meter or about the width of six carbon atoms or ten water molecules. A human hair is approximately 80,000 nm wide and a red blood cell is approximately 7000 nm wide¹. Nanotechnology can be defined as the science and engineering involved in the design, synthesis, characterization and application of materials and devices whose smallest functional organization in at least one dimension is on the nanometer scale or one billionth of a meter^2-5. It is a multidisciplinary field, which covers a vast, and diverse array of devices derived from Engineering, Physics, Chemistry and Biology and the cumulative efforts of all the disciplines is necessary for any significant outcome.

With the development of improved systems for monitoring environmental conditions and delivering pesticides appropriately, nanotechnology can further improve our understanding of the biology of different crops and thus potentially enhance yields. It can offer routes to plant disease diagnostics, insect-pest management and efficient pesticides utilization. Nanostructure catalysts will help in increasing the efficiency of pesticides allowing lower doses to be used. 

Introduction
Climate change, rapid population growth and limited natural resources such as water and soil pose significant global challenges today. There have never been such drastic repercussions on fruit output worldwide from climate change. In this sense, utilizing methods from the present era of nanotechnology can enhance fruit output and encourage sustainability. Fruit crops have a multitude of roles in world agriculture, making them essential. They supply vital vitamins, minerals and antioxidants that are necessary for maintaining human health and preventing disease. Fruit crops are important players in both local and foreign markets, bringing in large sums of money for farmers and generating a large number of job opportunities, especially in rural areas. Fruit orchards improve soil health and decrease erosion, which promote biodiversity and increase climate resilience. Furthermore, fruits are highly valued on a cultural and social level around the world, enhancing communal customs and culinary traditions. Fruit crops meet nutritional needs by diversifying diets and enhancing food security, which makes them essential
The term ‘nanotechnology’ is derived from the Greek word “nano,” which means dwarf. The development of nanotechnology is closely linked to a historic statement made by Nobel Prize winner Richard Phillips Feynman “There is plenty of room at the bottom”. Designing, characterizing, manufacturing and applying structures, devices and systems by manipulating their size and shape at the nanoscale scale is known as nanotechnology. A nanometer, abbreviated as nm, is one billionth of a meter, or 1×10-9 meters. Nanoparticles are structures with sizes between 1 to 100 nanometers. Nanotechnology may be very helpful in many areas of science and industry by utilizing the special properties of nanoparticles. Understanding and manipulating matter at the nanoscale is of special interest to researchers in the domains of science, medicine, agriculture and industry since materials at this scale frequently exhibit radically different properties from those at larger scales. Nanoparticles find extensive applications in the fields of biotechnology, electronics, textiles, biomedicine, communication technology, biotechnology and renewable energy. In the field of agriculture, innovative nanomaterials have demonstrated great promise for the creation of hybrid crop varieties, new high-efficient agrochemicals for crop protection and nutrition and new crop types through genetic engineering. Additionally, the use of nanomaterials enhances the production of nutraceuticals, the efficiency of pesticides and fertilizers, food processing, packaging, food safety and plant nutrition. It also reduces environmental pollution.

Nanotechnology
Nanotechnology is the study and manipulation of matter at the nanoscale, where unique phenomena allow for novel applications. Nanotechnology is the utilization of components smaller than 100 nanometers for manufacturing and bio-fabrication. This adaptable technique had a wide range of uses, from clothing manufacture to medical advancements such as cancer detection and treatment, among others. The name “nano” comes from Greek, which meaning incredibly small, and a nanometer (nm) is equal to 10 -9 m. Nanotechnology, also called as Molecular Manufacturing based Nanotechnology (MNT) as it involves experimentation and manipulating of nano particles, in order to design and build materials and devices with the basic structure in the nanometer scale. Nano material which can exist in the form of powder or liquid, exhibits significantly different physical and chemical properties at nanoscales compared to their micro size materials with similar chemical composition. Nano food refers to food in nanoparticles, nanotechnology techniques or tools are used during cultivation, production, processing, or packaging of the food.
The development of both organic and inorganic materials achieved through conversion and manipulation of the materials on an atomic and molecular scale [Grumezescu et al., 2018; Dera & Teseme, 2020]. By reducing the particle size below its outset to approximately 1–100 nm, the physical and chemical properties of the materials significantly differed when compared to the original materials despite they contain the same base materials. The unique
and incomparable properties of nanoscale materials are arise due to their high surface area-to-volume ratio compared to the microscale of the same materials [Grumezescu et al., 2018, Berekaa, 2015].

Nanotechnology is a field of study which deals with materials at nano-scale like nanometer which is one billionth of a meter (1 nm = 10-9 meter). At nano-scale, physics, chemistry and biology converge towards the same principles and tools. Nanotechnology is domain in science and technology, which is multidisciplinary in nature and any scientist, engineer, medical doctor can do research on nanotechnology. Presently, it is more of a science and less of a technology because of a lack of proper tools for studying the properties of nano-material. Nano-particles (NPs) are materials that are small enough to fall within the nanometric range, with at least one of their dimensions being less than a hundred nanometres. This reduction in size brings about significant changes in their physical properties with respect to those observed in bulk materials. The presence of naturally occurring NPs is implicated in interplanetary and interstellar space (Hochella Jr, 2002) as well as our earth in which life from the beginning has evolved in their presence (Becker and Reitmeijer, 2006). Currently it is observed that NPs can be synthesized from many biological organisms including plants.

Introduction
Antimicrobial resistance (AMR) is a mounting global crisis that threatens public health, food security, and development. The rapid spread of resistant pathogens has rendered many conventional antibiotics ineffective, leading to longer illnesses, increased mortality, and escalating healthcare costs(1)(2). The World Health Organization characterizes AMR as a top-ten global health threat, driven by overuse, misuse, and inadequate dosing of antibiotics. It already kills 700,000 people a year and is expected to kill 10 million people a year by 2050 if nothing is done about it(3). After penicillin was introduced to the market in 1941, the first signs of resistance appeared in staphylococci, streptococci, and gonococci. By 1942, Staphylococcus aureus, which was penicillin-resistant, emerged(4). Drug resistance must be effectively addressed for a few reasons, including public health, economic considerations, and the long-term viability of medicine. Maintaining the effectiveness of existing treatments for infectious diseases is an important objective(5). NDDS technologies have emerged as powerful tools to enhance the delivery, bioavailability, and targeting of antimicrobial agents, potentially reviving older antibiotics, and maximizing the efficacy of novel compounds(6). Parallelly, AI has found impactful applications in biomedical data analysis, drug discovery, and the rational design of therapies by processing complex datasets, predicting resistance patterns, and guiding the creation of new molecules. The integration of AI with NDDS marks a paradigm shift to optimizes delivery systems, guides individualized treatment plans, and advances the field towards precision medicine for infectious diseases (7). This chapter surveys the convergence of these technologies, exploring how AIenhanced NDDS platforms are reshaping the response to AMR. Additionally, it examines diverse AI models and algorithms employed in the prediction, optimisation, and management of drug resistance, enhanced by real-time case studies for thorough comprehension(fig1.).

History and Recent Developments

Richard P. Feynman in 1959 suggested that it should be possible to build machines small enough to manufacture objects with atomic precision. His talk “There is plenty of Room at the Bottom” is widely considered to be the foreshadowing of Nanotechnology. He delivered this talk at the annual meeting of the American Physical Society at California Institute of Technology (Caltech), In 1990, a team of IBM Physicists revealed that they could write (arrange) the letter IBM using 35 individual atoms of Xenon.

In 1992 a book entitled Nanosystems: Molecular Machinery, Manufacturing and Computation was published by Eric Drexler where he outlined a way to manufacture extremely high performance machines out of molecular carbon lattice (diamondoid).

Around year 2000, federal funding for Nanotechnology in the United States began with National Nanotechnology Initiative (NNI). NNI defined nanotechnology as dealing with materials with sizes between 1 and 100 nanometers exhibiting novel properties.

The Government of India, in May 2007 launched a mission on Nano sceince and nano technology (Nano Mission) with an allocation of `1,000 crores.

Nanotechnology, which deals with understanding and control of matter at dimension of roughly 100 nm and below, has a cross sectoral application and an interdisciplinary orientation. At this scale, the physical, chemical and biological properties of materials differ from the properties of individual atoms and molecules or bulk matter, which enable novel applications. Nanotechnology research and development is directed towards understanding and creating improved materials, devices and systems that exploit these properties as they are discovered and characterized

There are many applications of nanotechnology such as in the area of medicine, chemistry and environment, energy, agriculture, information and communication, heavy industry and consumer goods. The alleged potential of this technology has garnered the attention of both developed and developing countries across the globe. The US National Science Foundation (NSF) has listed it as one of six priority areas; it is one of the themesin the EU Framework Program for Research and Technological Development in Europe; and it has been the focus of research in countries worldwide.

Introduction

Plant diseases are one of the causes of concern in agriculture and horticulture industry. Estimated global crop loss due to plant diseases exceeds hundred billion US$ worldwide (Orke et al. 1994; Narayanasamy, 2010). Plant diseases are caused by microorganism such as fungi, bacteria, viral, viroids and phytoplasma. Most of the plant pathogens are transmitted through seed or planting material, which can spread secondarily through vectors and lead to severe loss to the crop. Among plant diseases, viral diseases cause serious crop losses and affect the quality of products, while the use of virus- free seeds and planting stocks results in a substantial increase in agricultural crop production (Waterworth & Hadidi, 1998; Strange& Scott, 2005).

Indian Agriculture is facing a wide spectrum of constraints such as burgeoning population, shrinking farm land, restricted water availability, imbalanced fertilization, low soil organic carbon, besides experiencing the fatigue of green revolution and vagaries of climate change. To address all the challenges ahead, we should think of an alternate technology such as “nanotechnology” to precisely detect and deliver the correct quantity of nutrients or other inputs required by crops that promote productivity while ensuring environmental safety. The word “nano” refers to the size of one-billionth of a metre or one-millionth of a millimeter in any one of the dimension. We are aware that all materials are made up of atoms which are the smallest units and for example, ten hydrogen atoms put together will measure one nanometer. As the size gets reduced, the surface area gets increased by several folds.

Nanotechnology has the potential to revolutionizethe agricultural andfood industry with newtools forthemoleculartreatmentof diseases, rapid disease detection, enhancing the ability of plants to absorb nutrients etc. Smart sensors and smart delivery systems will help the agricultural industry combat viruses and other crop pathogens. In the near futurenanostructured catalysts will beavailable which will increase the efficiency of pesticides and herbicides, allowing lower doses to be used. Nanotechnology will also protect the environment indirectly through the use of alternative (renewable) energy supplies, and filters or catalysts to reduce pollution and clean-up existing pollutants.

Introduction 
Modern energy policies place a strong emphasis on developing low-carbon energy sources while also ensuring that society has enough energy security. However, because fossil fuels play such a vital part in the global energy system, measures to reduce GHG emissions from fossil fuels must be found. Researchers and scientists are examining the characteristics of materials at the nanoscale level to identify compounds that might boost the efficiency of the energy sources we now use. The physical properties of several materials (such as strength, heat conductivity, and reflectance) alter at the nanoscale in comparison to their bulk properties. When compared to their bulk properties, several materials have distinct physical properties at the nanoscale (such as strength, electrical and thermal conductivity, and reflectance). several materials on a nanoscale. Additionally, materials with nanostructures are more suited for material-to-material interactions due to their higher surface to unit weight volume ratio. This is a useful characteristic as many chemical and electrical reactions take place at surfaces and are greatly impacted by the chemical composition of the reactant and the surface’s structure. The goal of nanotechnology research in the fossil fuel industry is to create novel catalysts that will improve the energy efficiency of the fossil fuel breakdown process. Given that heavy crude oil contains sulphur. The transformation of coal into liquid fuels for use in transportation requires the employment of several kinds of catalysts. Furthermore, because of their structure and the presence of electrons in their outer orbitals, which increases the potential for chemical reactions, the majority of typical catalysts used in cars today rely on valuable metals like platinum. Concerns about the supply of precious metals are raised by the industrialised world’s transport networks’ explosive expansion. Finding more affordable and readily available substitutes is imperative given the scarcity and high cost of these materials, and this will be emphasised in the researchers’ upcoming work. Additionally, because of their unique qualities that relate to the phenomena of corrosion resistance or their exceptionally strong qualities that are advantageous in turbomachinery components, nanomaterials are also helpful in power systems (1) . The application of nanotechnology in the production of resistant ceramics for use as insulators in gearbox lines results in smooth coatings that reduce the likelihood of fouling in cooling water intakes. Additionally, nanotechnology can improve a material’s conductivity and produce a superconductor at ambient temperature rather than a superconducting characteristic at very high temperatures. Renewable energy sources—also known as “green energy”—such as solar, wind, and ocean energy— allow countries to reduce their reliance on petroleum and address environmental problems related to the use of fossil fuels. In these technologies, nanotechnology is also employed. Mao and Chen concentrated their research efforts on developing nanotechnologies for a select group of renewable energy sources, including fuel cells, solar cells, hydrogen storage, and hydrogen generation. This research aims to provide a comprehensive comparative analysis of nanotechnology’s application to the development of sustainable energy systems, including nanomaterials, nanofluids, and nanostructures.

1. Introduction
Aquaculture is rapidly emerging as one of the most important sectors in global food production, supplying an increasing share of the world’s demand for high-quality protein. As global populations rise and overfishing depletes wild fish stocks, aquaculture has become essential in addressing the global food security challenge. The expansion of aquaculture, however, presents a new set of challenges, particularly in the formulation and delivery of efficient and sustainable aquafeeds. Fish and crustaceans, like all living organisms, require precise nutrition for optimal growth, immune function, and reproduction. In commercial aquaculture, ensuring that farmed species receive the right balance of nutrients through formulated feeds is crucial to achieving high productivity and minimizing environmental impact.
`Traditional aquafeeds are designed to meet the nutritional needs of farmed species; however, they face several obstacles when it comes to efficient nutrient delivery. The aquatic environment poses unique challenges for feed stability and nutrient bioavailability, including:
• Water Stability: Aquatic feeds must resist rapid breakdown when exposed to water, ensuring that nutrients remain intact until they are ingested by fish or crustaceans.
• Nutrient Leaching: In water, nutrients can leach out of feeds before they are consumed, reducing their availability and increasing waste.
• Complex Dietary Requirements: Aquatic species have diverse and often complex dietary needs, which must be met through balanced and targeted feed formulations.

Introduction 
The creation of molecular-scale machinery and gadgets at a few nanometers (10^-9 m), known as nanotechnology, has a substantial impact on this topic and dramatically reduces the size of a cell. To assess how nanoparticles affect the synthesis and production of biofuels, particularly biodiesels, a variety of nanomaterials, including nanofibers, nanotubes, and nanometals, are being used (1). Research on biodiesel has shown that utilizing nanotechnology and nanomaterials can be a useful approach to implementing low-cost techniques that improve manufacturing quality. Because of their tiny size and special qualities, such as a high surface area-to-volume ratio, catalytic activity, considerable crystallinity, adsorption capacity, and stability, nanoparticles (NPs) are useful in the generation of biodiesel (2). Because of their high potential recovery properties, metal oxide nanoparticles and carbon nanotubes are frequently used as nanocatalysts in the synthesis of biofuel and biodiesel (3, 4).This chapter critically evaluates the application of nanotechnology in the manufacturing and enhancement of biodiesel, addressing key challenges and potential advancements. Microalgae have been identified as a potentially useful feedstock for biodiesel production. The process of harvesting microalgae can be made much more efficient by employing a range of nanoparticles. Additionally, cost-cutting is facilitated by the recycling of nanomaterials and their incorporation into procedures including cell disruption, extraction, and harvesting. Furthermore, an array of nanocatalysts holds promise for enhancing the efficiency of biodiesel conversion (5). Classification of nanoparticles based on physical attributes as shown in (Fig.1). However, there is a growing emphasis on improving combustion efficiency and reducing harmful emissions in the engine industry and its associated fields. Several studies indicate that the introduction of nanoadditives into blends of diesel and biodiesel fuels has yielded significant results.

Introduction

Off late our understanding of the composition and functions of the human gut microbiota has increased exponentially and there is a great interest in producing healthy foods in particular that which have an impact in improving the gastrointestinal health such as probiotics, prebiotics and synbiotics. Many of these functional foods are dairy based and have been accepted greatly worldwide. Nonertheless, there has been a need for the development of nondairy probiotic, prebiotic and synbiotic products (Salmeron 2017). This has prompted food scientists and food industrialists to study the feasibility of applying other fermenting substrates such as cereals for the development of innovative nondairy fermented functional foods. The importance of including nanoscience and nanotechnology techniques for the creation of fermented cereal beverages that contain specific bioactive nanoparticles and their role in food packaging systems will be discussed.