eChapters

Plasma Activated Water (PAW) is an emerging technology that combines the reactive properties of plasma with water to create an effective medium for food preservation and decontamination. This chapter explains the principles of PAW generation and examines different generation systems, including atmospheric pressure plasma jets, corona discharge and dielectric barrier discharge. It explores applications of PAW in reducing post-harvest losses, extending the shelf life of highly perishable foods, supporting edible-coating formation, controlling undesirable enzyme activity and improving food safety. The chapter emphasizes the potential of PAW as a sustainable processing approach while discussing treatment conditions, food-matrix interactions, technological limitations and opportunities for future development in food preservation.

B.T.U.: It stands for British thermal unit. The heat required to raise the temperature of one pond of the water through 1^° F is called BTU.
Baby back ribs: Meaty pork ribs cut from the lower back rib section.

Bacitracin: A polypeptide antibiotic isolated from a bacillus (Bacillus subtilis or B. licheniformis) and usually used against gram-positive bacteria.

Back Bacon / Canadian bacon: Bacon cut from the loin that has little fat and is cut into round or oblong slices.

Water is a primary source of life and sustains all human activities such as domestic needs, agriculture, industries etc. The allocation and management of water resources is becoming a difficult task due to increasing demands, decreasing supply and diminishing quality. This calls for judicious use of water resources. The geomorphology describes the environment in which the hydrological processes operate. A strong mutual correlation exists between geomorphological variables and hydrological characteristics. Such relationship can be applied to both surface and groundwater regime. Thus the linking of geomorphological parameters with hydrological characteristics of the basin provides a simple way to understand the hydrogeomorphological behaviour of different basins and particularly of un-gauged basins.

During the last few years various applications of hydrogeomorphology with reference to the Geographic Information System (GIS) and various other techniques have emerged as an extensively effective tool for analyzing and prioritizing natural resource management alternatives. Because natural resource management problems are spatial in nature the GIS technology provides a tool for defining the extent of the problem, and facilitates the design and implementation of alternative management strategies. The GIS is used to estimate the hydrogeomorphological parameters of the watershed, which can be used in watershed planning and management at micro and macro levels. The results of the study can be utilized to infer the hydrological features like runoff potential, infiltration and groundwater recharge of the region. Some of the applications of hydrogeomorphological studies are described below:

Introduction

Turnip is an important root crop which is very popular particularly in northern India. It is a cool season crop mainly grown as a fall or winter crop in the hills and winter crop in the plains. Turnip (Brassica rapa L. syn. Brassica compestris var. rapa) belong to family Brassicaceae. It has a chromosome number of 2n=2x=20. Two main centers of origin have been indicated. The Mediterranean area is considered to be the primary centre of origin of European type/temperate types. While Eastern Afghanistan with adjoining areas of Pakistan is considered to be another primary centre with Asia Minor, Transcaucasus and Iran as secondary centre. The parents of cultivated turnip are found in the wild form in Russia, Siberia and Scandnavia. It was cultivated by the ancients and ranked next to grapes and cereals in Italy. It was introduced in England probably in 1590 and United States in 1606 (Yawalkar, 1980). Turnips are now cultivated throughout the world.

Turnips are botanically biennial herbs, but are cultivated as annual root crops for both animal and human consumption. The turnip root contains 41.6% moisture, 6.2 g carbohydrates, 0.5 g protein, 0.2 g fat, 0.04 mg thiamin, 0.04 mg riboflavin, 43 mg ascorbic acid, 30 mg Ca, 40 mg P and 0.4 mg Fe per 100 g of edible portion. The turnip green contains 15,669 IU vitamin A per 100 g (Aykroyd, 1963). Turnip is grown for its enlarged root as well as for its foliage. Extra seedlings from thinning are often used as greens. The fresh roots are consumed in salads or cooked as vegetable or used in pickles. The young leaves contain high amount of ascorbic acid and iron, rank second in vitamin A content and are cooked as greens.

The fleshy thickened underground portion of turnip is actually the hypocotyl. The colour and shape of this underground portion vary with the cultivars and the roots are flat globular to top-shaped and long. The colour of under ground portion may be white or yellow, while that of above ground portion may be red, purple, white, yellow or green. A distinct tap root and secondary roots arise from the lower part of the swollen hypocotyl. Thickening begins in the central part of the hypocotyle, followed by the upper and lower parts. Normally, the roots attain edible maturity in 40-80 days depending on cultivar and climatic conditions.

Flowers of the garden are brought indoors to enjoy the beauty. Fresh flowers are arranged in various styles according to one’s choice. There are two major and distinct styles of arranging fresh flowers, namely the European and the Japanese. Both the styles are greatly influenced by the place where they have been originated. Flowers are also dried and used for different arrangements. Fresh flowers are used as ornaments and also for making floral patterns on the floor called Rangoli.

Introduction

Potato is a wonder crop that can be grown under diverse range of agro-climatic conditions. Short duration; high yield per unit area and time and wide flexibility in planting and harvesting time are important virtues of potato that enable its inclusion in intensive cropping systems.

Potato is not an Indian crop but is a native of high Andean region of South America. It was introduced in India perhaps in 16^th or early 17^th century by either the Portuguese traders or the British missionaries. The present day cultivated potatoes (tetraploid, 2n=4x=48) in most of the world represent Solanum tuberosum spp. tuberosum and S. tuberosum spp. andigena, although it’s numerous diploid (2n=2x=24) relatives are still under cultivation in and around its primary and secondary centers of origin in South America (Pandey and Sarkar, 2005). The great Irish famine in the 19^th century, caused by late blight disease, nearly wiped out all the earliest potato introductions in the Europe and dramatically changed the potato cultivation and production scenario in rest of the world.

Unlike in European Countries 80% of the potato is grown in India under short day conditions as a short duration crop i.e. 70-90 days. The tubers are exposed to high temperatures during planting (September-October) and harvesting (February-May). The crop is irrigated and suffers mid day water stress. The potato varieties introduced from Europe were unsuitable for growing under the above conditions. Systematic potato research in India spanned over the last half-a-century of the 20^th century in the past millennium. The Central Potato Research Institute (CPRI), which was established in 1949, has bean mainly responsible for tremendous growth of potato production in the country. The indigenous technological advances made it possible to extend potato cultivation in almost all part of the country. Now in India, compared to the production, area and yield of potato in 1949-50, the increase over the same period was 1513%, 559% and 270% respectively (Naik and Thakur, 2007). According to the FAO, potato production worldwide stands at 329 million tones and covers more then 19 million ha.

Horticultural crops are highly perishable and the post-harvest loss is estimated to be 20-30% every year. There are various factors associated with post-harvest losses. It includes pre-harvest cultural operations, maturity index at which the produce is harvested, methods of harvest, pre and post-harvest treatments, packing materials and storage methods. Postharvest technology can be defined as the techniques which help to reduce the post-harvest losses.

Assessment of harvesting stage

Climate may be related to periodical events in plants life. The science dealing with this relationship is termed as phenology. The phenological data for horticultural crops include dates of planting, germination, flowering, fruit set, ripening and harvest. These dates are determined by the climatic factors prior to as well at the time of harvest. A useful phonological concept for the prediction of the harvest time is based on degree days. A “ Degree day” can be defined as a day when mean daily temperature is one degree above the minimum temperature for the growth of the plant. The minimum temperature for the growth of a particular plant is termed as “zero temperature”. Zero temperature vary according to crop. E.g. if zero temperature of a particular crop is 50°F, then a day with mean temperature of 68°F would provide 18° days. The degree days are also called as “heat units”. It can be determined by how many degree days are needed for the horticultural crop to reach harvesting stage.

To draw regeneration strategies for a city it is important to understand the city, therefore physical as well as social transformations, major events in history should be observed and analyzed. The strength of a regeneration technique lies in discovering the potential thrust areas for the city.

In order to have a better understanding of the urban setting of an Indian historic city, the ancient city of Allahabad is discussed. The history and development of this ancient city has been studied in a chronological fashion. This part of the book focuses on the period:

From antiquity to the revolt of 1857, city’s inherent potential as a popular pilgrim center, and also the phenomenon of Kumbh. Further exploring the sacredness of Allahabad. Why Allahabad is regarded as a Tirthraj? And what sacred elements constitute the ‘Prayag’ of olden times? Apart from Sangam, confluence of Ganga, Yamuna and Saraswati, the city is also known for Temples, namely, the Bharadwaj Ashram, Nag Basuki, Mankameshwar.

In conventional breeding for genetic upgradation of yield and other desirable attributes of crop plants, favourable assemblages of genes from two or more parents are made to develop a commercial variety. The important steps of this exercise are: locating the right kind of parents that possess the desired characteristics; making crosses between or among them; handling the segregating generations in an appropriate manner so as to identify the required recombinants; stabilizing the recombinants and finally evaluating their performance in a series of trials. This approach has been very successful in the sense that it has provided increments in productivity continuously. In an analysis done by the American Society of Agronomy on the genetic contributions to yield gains of five major crop plants, it was concluded that sorghum yields increased due to genetic improvements at the rate of 1.2 per cent per year between 1950 and 1980. For corn, the corresponding yield increase was 90 kg/ha/year between 1930 and 1980, which is equivalent to a rate of increase of 1 per cent per year. It was also estimated that 70 per cent of the total gains in corn yields are attributable to genetic improvement and 30 per cent of the yield gains are due to non-seed inputs like fertilizers, pesticides and cultural practices.

In India also, the improvement of productivity of the major food, fibre and several cash crops has been very impressive. The success is due to intensive research in plant breeding resulting in the evolution of varieties with very high genetic yield potentials and the concurrent support from other disciplines that has provided the package of practices which help in the realization of the created potential. In all crop plants, where the breeding efforts have been intensive, the phenomenon of plateauing of yields has become apparent. Wheat provides, perhaps, the best example of the situation. Since the 1970s, when the gains from genetic restructuring of the plant type had been established, major yield increases have not occurred. Since the demands of the future will be undoubtedly much larger, and additional cultivable land will not be available, ways will have to be found for a sizable increase in productivity per unit area in the immediate future. This requirement calls for the development of strategies and procedures that will make dramatic yield gains possible. For meeting the challenge, a continuous search is being made for innovative methods which will give yield increments of a much higher order than those provided by the conventional approach. In this chapter, an attempt has been made to introduce some such promising approaches.

9.1 The Cartagena Protocol on Biosafety: Objectives and Implementation
9.1.1 Introduction
The Cartagena Protocol on Biosafety (CPB) is a pivotal international legal instrument under the Convention on Biological Diversity (CBD), dedicated to regulating the transboundary movement, handling, and use of living modified organisms (LMOs) derived through modern biotechnology that may adversely affect biological diversity, human health, or the environment. Adopted in January 2000 in Cartagena, Colombia, and entering into force on September 11, 2003, the Protocol represents a global consensus on biosafety governance amidst the rapid expansion of genetic engineering technologies.
9.1.2 Objectives of the Cartagena Protocol
The principal objective of the Cartagena Protocol is:
“To contribute to ensuring an adequate level of protection in the field of the safe transfer, handling and use of living modified organisms resulting from modern biotechnology that may have adverse effects on the conservation and sustainable use of biological diversity, taking also into account risks to human health, and specifically focusing on transboundary movements.” (Article 1, CPB)
The core objectives can be summarised as
• Ensuring biosafety in the context of international trade of LMOs.
• Protecting biodiversity from potential risks posed by LMOs.
• Safeguarding human health against unintended exposure to transgenic organisms.

3.1 Introduction

Fish are the only true aquatic vertebrates. These exploit every nook and cranny of the water domain. They are notable for their vast numbers and perplexing forms. In the phylogenetic history of vertebrates, this is the first group of animals to develop biting jaws. The origin, evolution and interrelationships of fishes are not fully understood due to incomplete fossil records and a scarcity of primitive types. The early appearance of bone, cartilage and enamel-like substances was crucial in the evolution of fish.

Delhi, Capital City

Delhi, the capital of India has a unique urban sprawl. This city is an urban maze comprising of old heritage areas like walled city Shahjahanabad, Lutyens New Delhi and the newer areas built after independence. Delhi has been the capital of our country for centuries. In 1947 it became the capital of free and independent India. In 1956 was made a Union territory and National Capital Territory on 1^st February’1992.

Situated between the Himalayan and the Aravali range. It is surrounded on three sides by Haryana and on the east by Uttar Pradesh. The total area of the territory is 1,483 sq.km. The climate is extreme dryness with intense hot summer and extreme cold winters. NCT has 209 villages and 7 towns including the Delhi Urban Agglomerations.

Introduction

The rapid pace of urbanisation and abrupt growth of population in few large cities in India has become a standing menace to balanced socio-economic development of India. The rapid pace of development in cities had always attracted investors and entrepreneurs to set their enterprises leading to creation of employment opportunities in these cities. On the contrary, the lack of opportunities in rural areas has created a backwash effect leading to creation of slums in and around potential growth poles (i.e. large cities and growing cities).

For the intelligent appreciation and understanding of various reactions and processes associated with soils a little basic knowledge of chemistry is helpful. If the reader follows this chapter carefully, the working knowledge of chemistry thus acquired will enable him to follow all the complicated chemical reactions taking place in the soil.

What is Chemistry? Chemistry is the science that deals with the chemical changes. What is a chemical change? When two or more substances combine to give a substance entirely different in properties to the individual components, the change is called a chemical action to distinguish it from the purely physical action in which the properties of the product are intermediate between the components. The latter naturally can be separated easily by mechanical means. For instance a mixture of sand and water can be separated by passing through a filter, and a mixture of salt and water can be separated by evaporating the water when the salt is left as residue. But water itself is a compound of two gases: oxygen and hydrogen. Its properties are totally different from its components. We can freeze it, evaporate it and perform a host of other operations on it without changing it in the least.

Atoms and molecules: The whole science of chemistry rests on atoms and molecules If these are understood clearly, basic chemistry is reduced to simplicity. Take a familiar object like gold or silver and go on subdividing it into smaller and smaller bits. You will reach a stage that further subdivision will become impossible. You will have reached the primary units, the elementary bricks of which the substance is made up, namely the “Atoms”. An atom in chemistry is the smallest brick from which all material substances in the world are made up. Not that you can see an atom. It is so small that even the most powerful microscope will nut reveal its presence. You could perhaps just see a collection of a million atoms as a tiny spot of light if it is illuminated with a powerful beam of light. Even then you could not say if it was round, square, or oblong. However, you need not look for any such tangible proof to believe in the existence of atoms. Even pure logic would compel you to stop somewhere if you started with an imaginary division and sub-division of a substance. You could not go on ad infinitum. Even the ancients, who knew no modern chemistry, believed in their existence purely from philosophical reasoning.

Kharif onion helps maintain supply and stabilize prices when stored rabi onions become scarce. Its production is nevertheless challenged by high temperature, excessive rainfall, humidity, unsuitable varieties and limited availability of quality seed. Onion is biennial for seed production and requires bulb production followed by replanting for flowering and seed formation. Its protandrous flowers encourage cross-pollination, making adequate isolation and pollinator activity essential. This chapter explains short-day varietal requirements, bulb selection, vernalization, isolation, field management, pollination, harvesting, seed processing and storage. Improved infrastructure, organized production and farmer training are required to ensure continuous supplies of genetically pure, high-quality kharif onion seed.

In agriculture, sensors can monitor soil, water, vegetation, and environmental conditions. They assist farmers in enhancing crop quality, optimizing water usage, and making informed fertilizer choices.
CROP MONITORING
The primary function of remote sensing in agriculture is to assess crop health. Optical (VIR) sensing enables the observation of wavelengths beyond the visible spectrum, such as infrared; these wavelengths are highly responsive to crop vitality, damage, and stress. Recent advancements in this technology have enabled farmers to monitor their fields and execute prompt crop management decisions. Remote sensing for crop identification facilitates the detection of crops impacted by weather-related circumstances, pests, and other factors.
Pest Detection
Drones can administer insecticides and herbicides with greater precision, reducing chemical consumption and environmental repercussions. Drones can minimize chemical application by targeting specific regions impacted by pests or weeds, thereby safeguarding beneficial insects and mitigating the danger of pesticide resistance. Drones can also be utilized to deploy biological control agents, including beneficial insects or fungi, to address pest issues in an environmentally sustainable way.
Irrigation Management
A primary application of UAV technology in smart agriculture is crop irrigation management, which enhances water consumption efficiency by delivering the precise quantity of water at the most suitable location and time. UAVs equipped with suitable sensors can identify regions of a crop that necessitate supplementary irrigation. This positively impacts overall crop productivity. The

Foods are classified into three groups on the basis of ease of spoilage. Spoilage meant a condition produced by the excessive growth of microorganisms leading gradually to decay or decomposition. The stable or non perishable foods like sugar, flour, grains and dry products do not ordinarily spoil unless handled carelessly. The semi-perishable food like potatoes, onions, apples, waxed fruits, fruits and vegetables having thick skin can be protected from spoilage if properly handled and processed. The perishable foods, like livestock products, seafood, and poultry items, dairy products, and most fruit and vegetables spoil readily unless special preservatives methods are used. The spoilage of foods may be caused by molds, yeast, bacteria, enzymes, food constituents and insects. The use of food additives have become necessary to prevent spoilage other wise caused by the growth of bacteria, yeasts and molds.

Food spoilage usually refers to undesirable changes occurring in food due to the action of micro-organisms, insects and enzymes. The original nutritional value, texture, flavour of the food are damaged, the food become harmful to people and unsuitable to eat. Today however, preservation has used such factors as temperature, water activity, pH, gases, organic acids, salts, antibiotics, irradiation, packaging and various combinations of these factors. Foods vary greatly in the length of time for which they can be held in their natural form without spoilage. Preservatives retard product spoilage caused by mold, air, bacteria, fungi or yeast. Bacterial contamination can cause food-borne illness, including life-threatening botulism.

Detecting pathogens in fish is a vital component of maintaining health in aquaculture systems, as it enables the early identification of diseases and supports swift, effective responses. Fish can be affected by a variety of pathogens, including viruses, bacteria, fungi, and parasites. To ensure that pathogen detection is both reliable and safe, it is critical to implement stringent biosafety measures and adhere to Good Laboratory Practices (GLP). These frameworks are designed to safeguard personnel, protect the surrounding environment, and produce dependable, repeatable diagnostic outcomes.
Good Laboratory Practices represent a set of globally accepted guidelines that promote the generation of consistent and trustworthy data. These practices are fundamental across various applications, from academic research to industrial diagnostics and regulatory processes. Biosafety, in the context of detecting fish pathogens, involves strategies to prevent accidental exposure to infectious agents. Laboratories conducting such work must operate at biosafety levels appropriate to the organisms being examined—typically BSL-1 or BSL-2, with BSL-3 reserved for highly virulent pathogens.
A key element of biosafety is the preliminary assessment of risk. Before starting any diagnostic or research activities, lab personnel must evaluate the nature of the pathogen, its transmission method, level of pathogenicity, and required containment procedures. This evaluation informs the use of protective measures, including gloves, lab coats, eye protection, and in specific cases, respiratory masks. The physical design of laboratories also contributes to biosafety. Proper infrastructure should include accessible handwashing stations, efficient ventilation systems, biosafety cabinets (Class II or above), and controlled entry to reduce the risk of exposure and contamination. Activities that may generate aerosols—such as homogenizing tissues or centrifuging samples—must be carried out within biosafety cabinets to maintain safety.

Bacterial culture methods are key to diagnosing and confirming bacterial infections in fish. These techniques involve the isolation and growth of bacterial pathogens from fish tissues or environmental sources under controlled lab conditions. The effectiveness of these methods depends greatly on accurate sample collection, careful handling, and the correct choice of culture media and incubation parameters.
Samples are aseptically collected from infected fish—typically targeting tissues such as the kidney, liver, spleen, gills, and any visible skin lesions. In live fish, sterile tools are used for tissue extraction, while in dead fish, internal organs should be harvested promptly after death to reduce contamination. For surface-level infections, swabs taken from mucus or skin lesions are also helpful. To maintain bacterial viability and minimize contamination, samples must be kept cool during transport.
In the laboratory, samples are inoculated onto culture media. The type of media used depends on the suspected pathogen. Common general-purpose media include Tryptic Soy Agar (TSA), Nutrient Agar (NA), and Brain Heart Infusion Agar (BHIA). For selective isolation, media such as MacConkey Agar (for Gramnegative bacteria), TCBS Agar (for Vibrio species), or Edwardsiella Isolation Medium are utilized. Enrichment broths like Alkaline Peptone Water or Buffered Peptone Water may be used to boost bacterial numbers before plating.
Incubation typically occurs at 25°C to 30°C, with most fish pathogens growing well in this range. Plates are monitored over 24 to 72 hours. Bacterial colonies are assessed for physical traits such as size, shape, color, and hemolysis patterns, particularly on blood agar.

INTRODUCTION OF ASSESSMENT OF SOIL MOISTURE LEVELS IN DRONES
In contemporary agriculture, effective water management is essential for optimizing crop yields while reducing resource consumption. Drones have become a transformative device for evaluating soil moisture levels and identifying irrigation requirements, providing farmers with accurate, real-time data to enhance water utilization. Equipping drones with sophisticated sensors, including thermal, multispectral, and LiDAR, enables them to assess soil conditions over extensive regions, offering insights on moisture fluctuations and pinpointing arid areas in fields. This data-driven methodology not only improves the precision of irrigation schedules but also mitigates water waste, fosters sustainability, and boosts overall crop vitality. Utilizing drones enables farmers to make informed decisions, resulting in more efficient and cost-effective irrigation methods.
Drone soil analysis utilizes drones fitted with specific sensors to gather data on the physical and chemical characteristics of soil. This data may encompass details regarding soil texture, organic matter content, nutrient concentrations, moisture levels, and pH. Various sensors are employed for distinct soil qualities. Multispectral sensors acquire data across several wavelengths of light, offering insights on plant health and soil properties. Hyperspectral sensors acquire data across numerous tiny spectral bands, yielding enhanced insights into soil composition.
Thermal sensors quantify soil temperature, serving as an indicator of soil moisture content and additional characteristics. The data is subsequently processed and analyzed to generate comprehensive maps of soil variability within a field, offering farmers critical insights to inform their management decisions.