eChapters

Abstract

The abiotic stresses viz. drought, high temperature, water stress, salinity, heavy metal contaminations etc. cause variable effect on nitrogen (N) mineralization, its uptake and transport, assimilation and remobilization, and subsequently the growth and productivity of plants. The affect is generally negative, however, the magnitude of decrease in various processes is dependent on genotype, age of the plant, tissue under investigation, type and extent of the stress and other agro-climatic conditions. Biological nitrogen fixation and nitrate assimilation appear to be more sensitive than the ammonium assimilation possibly because re-assimilation of ammonia released in the tissue due to catabolism of N molecules is essential for the survival of plants. In addition, synthesis of stress metabolites (proline, glycine betaine and poly amines) is directly dependent on the availability of primary amino acid, glutamic acid. There are few well coordinated and detailed studies, determining the key factors involved in regulating NUE under abiotic stresses.

Abstract

Nitrogen is an important nutrient for growth and development of plants. Presence or absence of nitrogen often affects the amino acid composition of proteins, which may in turn change its nutritional quality. Increase in the elevated CO₂ concentration and temperature associated with future climates are expected to affect the nitrogen use efficiency (NUE), growth and grain yield of wheat (Triticum aestivum L.). Atmospheric CO₂ is steadily rising from the current level of approx 384 µLL^-1 and is expected to rises upto 550 µLL^-1 by 2050. It is predicted that the global temperature would increase by 1.5- 4.5^oC. The main future challenge is to feed nine billion people under an altered climate, as this increase in global temperature would affect, negatively or positively the future crop productivity including wheat depending upon the geographical location. The present book chapter summarizes the potential impact of elevated CO₂ and temperature on NUE of Triticum aestivum L.

Greek scientist Theophrastus (300 BC) advocated legume rotation. As early as 1750 AD it was known that plants require Nitre. Louis Pasteure in 1860 AD stated the possibility of atmospheric nitrogen fixation by microorganisms. Hellriegel and Willfarth (1886) strengthened the views of Louis Pasteure by his observations on the irratic behaviour of legumes to nitrate nutrition. Beizerink (1890) isolated the organism involved in nitrogen fixation. The systematic field experiments conducted at Rothemstead experimental station showed that the plants respond to inorganic nitrogen.

ROLES OF NITROGEN IN CROPS

Plants contain 1-5 per cent nitrogen by weight. It is a structural component of chlorophyll, aminoacids and proteins. Plant proteins contain 16 per cent nitrogen (6.25 × %N = protein). Plants are dependent on protein for their propagation. Cereals possess a threshold protein level below which grain will not form.

Introduction

The enzyme nitrogenase is produced in all N₂-fixing systems. The activity of nitrogenase enzyme relates to the biological nitrogen fixation, which can be determined directly by measurement of fixation product, ammonia and the further products of its assimilation. The activity can also be measured indirectly by measuring the ability of nitrogenase to reduce the number of substrates other than N₂. Substrates such as nitrous oxide (N₂O), azide ion (N₃^- ), cyanide (CN^-) and isocyanide (NC^-) are reduced and the requirements for ATP and reductant are similar to those of N₂ fixation. The reduction of protons to molecular hydrogen has been used to measure the nitrogenase activity.

Dilworth (1966) and Schollhorn and Burris (1966&1967) observed the inhibitory effect of Acetylene (C₂H₂) on N₂ fixation by extracts of Clostridium pasteurianum. Dilworth (1966) further observed by mass spectrometry that the C₂H₂ was reduced to C₂H₄. Both gases are readily measured at very low concentrations by gas chromatographic techniques.

Nitrogen (N) is an essential element for all forms of life because it is a major component of amino acids, which are the building blocks of proteins, nucleic acids such as deoxyribonucleic acid (DNA) and other cellular constituents. It is a structural component of chlorophyll in plants, which is used in photosynthesis to make their food. Nitrogen ranks first among the essential nutrient elements required for plants. Its careful management is very important for crop production.

Nitrogen exists as two main stable isotopes (isotopes of a given element have the same number of protons but different numbers of neutrons in each atom) namely, ¹⁴₇N (abundance 99.63%) and ¹⁵₇N (abundance 0.37%). These can be separated by chemical exchange or by thermal diffusion. Both ¹⁴₇N and ¹⁵₇N isotopes of N are stable isotope which do not disintegrate as in case of other radioactive isotope like ³² ₁₅P. There are four other isotopes of N viz. ¹²₇N, ¹³₇N, ¹⁶₇N and ¹⁷₇N, mostly are radioactive having very short half-life and not used in agriculture. The ¹³₇N has a half-life of about 10 min, while other three have half-life of only few sec. The ¹⁵₇N (tracer) isotope is used as a tool for determination of pathways of transformation of N in soil or plant or animal.

The major part of N comes from atmosphere (air). On the basis of volume by volume (v/v), air contains about 78-79% N₂, 20-21% oxygen (O₂) and 0.03% carbon dioxide (CO₂). Most living organisms cannot utilize N₂ gas present in the atmosphere directly because it is present as di-nitrogen form or di-atomic form (N≡N). It is called as an inert gas. Therefore, to break this triple bond, huge amount of energy is required. After breaking of triple bond of N₂ molecules, it combines with other element particularly with O₂ or hydrogen (H) to form oxides of N (NO, NO₂, NO₃ and N₂O) or ammonia (NH₃). Thus, breaking of triple bond is must for forming NH₃ which further can be used as the key material for almost all fertilizers. Therefore, the fixation of N₂ is vital for production of all nitrogenous and complex fertilizers like NP/NPK and nitrophosphates.

Nuclear Magnetic Resonance spectroscopy is a powerful and theoretically complex analytical tool. The nuclei of certain isotopes are continuously spinning with an angular momentum, which can give rise to an associated magnetic field.

Edmond Nocard (French Veterinarian) first isolated filamentous aerobic bacteria from clinical cases of ‘bovine farcy’ in 1888. In 1889, the genus Nocardia was formed by Trevisan, and the organism described by Nocard was named as Nocardia farcinica. In 1891 another similar organism (Nocardia asteroides) was isolated by Eppinger from a human brain abscess. Initially it was thought that they are the same organisms. Latter on the basis of the molecular studies their separate entity is established.

            However, 80 years later, re-examination of Nocard’s original isolate revealed two different filamentous organisms, one of which was Mycobacterium and the other was Nocardia. The cause of ‘bovine farcy’ has been subsequently attributed to Mycobacterium. Latter, it is proved that Mycobacterium farcinogenes is the actual causative agent of ‘bovine farcy’.

Nocardia has the ability to form gram-positive, branching filaments of less than 1um in diameter. It is closely related to Corynebacterium, Mycobacterium and Rhodococcus species
History Nocard described this organism in 1888, following its isolation from a case of bovine ‘farcy’, hence the name of the type species: Nocardia farcinica.
Natural Habitat
Nocardia species are soil borne saprophytes
Morphology
They are gram-positive, obligate aerobes ability to form branching filaments. Some produce true mycelia and some strains are acid-fast. All species are nonmotile. Gram stained smears from lesions revealed gram-positive branching filaments that often show fragmentation into coccobacillary elements. The modified ZN stained smears exhibit a similar morphology but most of the filaments retain the carbol-fuchsin dye and stain red.
Cultural Characters
The Nocardia species grow very well in blood agar incubated aerobically at 370C for upto 7 days. Inoculate the suspected colonies from blood agar into Sabouraud dextrose agar (SDA) and incubated at 370C for upto 10 days.

Noise has become a very important “stress factor” in the environment of man especially with regard to health. However, not much attention is being paid towards its elimination or control in dairy plants. The harmful effects of noise(s) in the workplace as an occupational hazard is really a cause of great concern. Apart from reducing the work efficiency, in the long run it causes serious auditory and non-auditory effects on the worker. Thus, it becomes imperative to set legal standards in workplaces to be monitored constantly to avoid or reduce the health hazards of the workers.

Noise and Noise Pollution

Noise is often defined as “Unwanted Sound” or “Undesirable Sound”. The “Noise Pollution” has been coined to signify the vast cacophony of sounds that are being produced in the modern life, leading to health hazards.

A sound is a form or energy. Therefore, it has to have an impact on life in one way or the other. Most of the organisms of Kingdom Animalia communicate with each other creating a peculiar sound. Creating sound is inevitable for performing various life activities. Every animal species has the characteristic sound it reflects through its behavior. Whatever all the animals communicate through creating specific sounds is all-natural. Nature, as such is a stupendous repository of a diversity of sounds, all being specific characteristics of life.

Human behavior, however, is highly evolved in terms of creating a variety of sounds. Communication, entertainment, and many behavioral activities are expressed through creating distinctive sounds. There is a difference between natural sounds and the ones human beings create or can create. Humans often generate sounds, not just in a natural manner, but also with various technical aids, that often become unpleasant what we call noise. Creating noise, or noise pollution, seems to be an exclusive characteristic of the human species. Of course, there could be some natural sounds that might occasionally cause havoc to life. Examples are the sounds generated at the time of the volcanic eruption, during an earthquake

Introduction
The term “noise” originates from the Latin word “nausia.” There are negative repercussions of noise pollution on our bodies and minds. In general, the amount of noise and the length of exposure determine how much noise pollution there is. These
days, noise is a constant in our lives due to the advancements in industry, technology, and machinery.
Definition
Unwanted sound released into the atmosphere that poses a health risk is referred to as noise pollution.
The term “unwanted or unpleasant sound that causes discomfort” refers to nose pollution.
Another definition of noise pollution is “wrong sound, in wrong place at wrong time.”
Distinction Between Noise Pollution and Sound Pollution:
Sound is defined as the lowest level of 50 dB that is pleasant to listen to. Noise pollution is defined as any sound that is louder than 95 dB and bothersome to listen to.
Sources of Noise Pollution
1. Factories and industries, community and religious activities, and modes of transportation (air, train, and road) are the primary sources of noise pollution.
2. In metropolitan regions, automobiles, factories, industries, railroads, and airplanes are the main man-made sources.

For the hundreds of thousands of years that we lived on earth,there were few rapid changes in the atmosphere. It is only in recent times, particularly since the Industrial Revolution, that our activities on earth began to modify our environment in a significant manner.  Industrial pollution has affected the water we drink, the air we breathe, and in general,the biosphere itself.Now noise has joined the bandwagon of pollutants.

Noise certainly comes within the scope of definition of a pollutant that “..... any undesirable change in the physical, chemical or biological characteristics of our air, land and water that may or will harmfully affect human life or that of any other desirable species or industrial process, living conditions or cultural assets, or that may or will waste or deteriorate our raw material resources.”

Taxonomy

Taxonomy is the science that deals with the identification, nomenclature and classification of organisms.

Systematics

It is scientific study of organisms with the ultimate object of characterizing and arranging them in an orderly manner.

Classification

Classification is the ordering of organisms into groups and systems, based on their morphological, phylogenetic and physiological relationships.

Nomenclature

It is the system of assigning names to the taxonomic groups or organism according to international rules.

Principle
(Method of Patterson 1963)

The albumin bound long-chain fatty acids of plasma are usually referred to as unesterified or non-esterified fatty acids (NEFA) to distinguish them from the long-chain fatty acids present in the esterified form in neutral fats and phospholipid. In the case of ruminant plasma, it is also important to distinguish these “free fatty acids” from the small molecular weight steam volatile fatty acids of dietary origin which are found in the free state as the carboxylate ion.

Basically, the method of Dole (1959a) has been used to estimate plasma NEFA employed by Annison (1960), which is to evaporate the heptane extract of plasma to dryness before titration. This is conveniently done in a stream of nitrogen on a warm hot plate rather than by evaporation in vacua. In place of the 0.05 M- phosphate buffer (pH 6.0) of Annison or the 0.02 N-H₂SO₄ used by Dole (1956a), in the present method 0.02 N-HCI is used as an extraction medium. This is preferred because owing to its volatility, it is less likely to cause serious accidental contamination of the supernatant heptane layer.

26.1 Introduction

The egg’s primary non-food purpose is to help the species reproduce. The quantity of eggs required for the hatching of chicks has increased significantly due to the substantial rise in meat consumption. The USDA has made a list of a few other non-food use for eggs.

Inedible eggs are often used as animal feed and plant fertilizers. Eggs are also fed to show animals, such as dogs and horses, to improve the glossy sheen of the fur coat. Industrial egg albumen is used in finishing several types of leather, notably coloured stock, and glazed. production of vaccines in chick embryos is one of the key uses of eggs, as per various researchers.

In the past several Non infectious diseases (NIDs) of fish and shellfish have been observed in natural waters. However, with expansion in fresh, brackish and marine aquaculture including aquarium keeping world wide there is a phenomenonal increase in incidences of NIDs and economic loss. Common Non infectious diseases (NIDs) in fish are 1.Gas bubble disease(GBD), 2. Body deformities(BD), 3.Nutritional diseases(ND) and 4. Disease due to harmful algal bloom(DHAB)

1. Gas Bubble Disease (GBD)

Gas bubble disease (GBD), is caused by supersaturation of gases in water. The gas involved is nitrogen in most cases and rarelycarbon dioxide and oxygen can cause GBD as these two gases are used by fish tissues and readily processed.Oxygen can cause gas-bubble disease at about 350 per cent air saturation, but nitrogen can cause the disease even below 118 per cent. GBD becomes prominent whenever there is a change in temperature, pressure and turbulance in aquatic environment, leading to disturbance in metabolism of f ishes. Bubbles of gas may form in the eyes, skin, gills, fins and within the internal organs where small bubbles may coalesce into larger bubbles.Fish absorb the excess gas, which forms bubbles in the small blood vessels, due to pressure changes inside their body. Gas bubble disease (GBD), is seen both in captive and wild fish. When bubbles affect internal organs, some fish may diesometimes without obvious clear clinical signswhile other fish may be chronically affected, and have a reduced appetite, activity and buoyancy. GBD can also cause secondary bacterial, viral or protozoan infections.All fish and shelfishspecies as well as amphibians and aquatic invertebrates are susceptible to GBD.Sensitivity of fish to GBD in terms of pathology and mortality varies with species and age.

11.1. BORON TOXICITY
11.1.1. Introduction

Boron (B) is essential micro-nutrient. It is necessary for cell wall formation, membrane integrity, calcium uptake and carbohydrate synthesis and transportation. B affects many functions in plants, which includes flowering, pollen germination, fruiting, cellular activities (division, differentiation, maturation, respiration etc.), water relationships and the movement of hormones. It is not translocated and leached easily from soils.

Nitrate – Nitrite Poisoning
Sources

Accidental ingestion/grazing of
Plants which are Nitrate accumulators