eChapters

8.1 Introduction

The normal distribution was discovered by De Moivre in the year 1733. Normal distribution is probably the most important of all theoretical distributions for the reason that so many physical measurements and natural phenomena have observed frequency distributions which very closely resemble the normal distribution.

An understanding of normal values of electrocardiographic indices of healthy dogs and cats is utmost necessary for interpreting an abnormal electrocardiogram. The criteria for normal configuration and measurements of the waves, segments and intervals in an electrocardiogram of healthy subjects provides basis for detecting abnormalities in the electrocardiogram of the ailing animals. The electrocardiogram, like other diagnostic aids, is not an absolute indicator of health and disease and therefore it is required to be interpreted in conjunction with patient’s history, clinical manifestations, and the results of other investigations.

Abstract

The research work was conducted on the moisture dependent physical properties of kidney bean (White speckled kidney beans) were analyzed. Length, width, thickness varied between 1.53-1.90, 0.79-0.94, 0.58-0.76 cm for kidney beans, respectively. Arithmetic mean diameter and geometric mean diameter were 0.96-1.19 and 0.88-1.11 cm₂, respectively. Volume, Sphericity and surface area ranged between 0.44 -0.87 cm₃, 0.57-0.60 and 2.91-4.68 cm₂ for white speckled kidney beans, respectively. The kidney bean was soaked in normal water with initial moisture content 14.22 % (db) upto 270 min and attained the final moisture upto 83.23 % (ab).

Normal bacterial flora is the bacteria which live on another living being (human or animals) or inanimate object without causing infection

Opportunistic bacteria are part of normal bacterial flora which can produce infection during lowered immunity (immunosuppression) of host or when transferred into other parts of the body

Saprophytic bacteria live on dead or decaying organic matter Infection

Infection can be defined as a pathological condition occurred due to multiplication of microbes.

Intoxication can be defined as pathological condition due to ingestion of microbial toxin (e.g. aflatoxin produced by Aspergillus fungus).

Mycetism is the pathological condition due to ingestion of fungus (e.g. toxic mushroom) which produces toxicity within the body of the host

Norman Borlaug’s early upbringing in an Iowa farm and his experience of hardship during the US Depression of the early 1930s instilled in him the desire to bring science to address the problems of low farm productivity, poverty and hunger. After completing his Ph.D degree in Plant Pathology at the University of Minnesota in 1942, he joined the Rockefeller Foundation’s agricultural programme in Mexico, leading to the birth of the International Maize and Wheat Improvement Center (CIMMYT). There he began his work on wheat, concentrating on the control of stem, stripe and leaf rusts, the important diseases of the crop. He introduced a multi-pronged approach to manage the rust fungus, including the development of composite varieties characterised by phonotypic identity but genotypic diversity with reference to resistance to different races of the pathogen, an approach conferring enduring resistance as a result of reduced pressure on the fungus to mutate and create more virulent strains.

Pulses occupy an area of 0.18 m ha with total production of 0.12 mt in NE Region. This region is one of the potential areas where pulse production can be increased horizontally by utilizing a part of 16.72 lac ha area under jhum cultivation (Bujarbaruah, 2004). Considering that pulses could be grown mixed with other crops and their feasibility for relay cropping; the area and production of pulses could be increased vertically. Intercropping system not only covers the risk but also give a bonus yield of pulse crop in addition to normal return of the main crop. Maximum area and production of 0.11 m ha and 0.60 mt which is 60.90 % and 51.28% of the total area and production of the region, respectively is contributed by Assam only. After Assam, Nagaland and Tripura are the major pulse growing states having 30 and 8 thousand hectares of area with 28 and 5 thousand tonnes of production, respectively. In Meghalaya, pulses are grown on 5 thousand ha area and 4 thousand tonnes of pulses were harvested. Other states of this region grow pulses in about 6-7 thousand hectares area and produce 3-6 thousand tonnes (Table 1). The productivity of pulses in all the states is 1000 kg/ha except Assam and Manipur where its productivity is only 500 kg/ha. The average pulse productivity of all the NE States (661 kg/ha) is higher than that of whole India (637 kg/ha)

Northern Blot

The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.

Introduction

The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression levels during differentiation, morphogenesis, as well as abnormal or diseased conditions. Northern blotting involves the use of electrophoresis to separate RNA samples by size and detection with a hybridization probe complementary to part of or the entire target sequence. The term ‘northern blot’ actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane; however the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot.

ABSTRACT

The popular use of local plant wealth among the rural people in Hamirpur district of Himachal Pradesh reflects their greater interest in the traditional medicine and other traditional and commercial purposes. Hamirpur, one of the pioneer Himalayan district is a rich repository of medicinal flora. It is the smallest district of Himachal Pradesh due to its area, surrounded by thick forest area which is rich in diverse vegetation. Medicinal plants are used in the Ayurvedic, Unani and other traditional systems of medicine and in plant-based pharmaceutical industries. The patterns of the use of the local medicinal herbs for the treatment of various diseases and ailments have been an old practice. But the people of the district still depends upon the uses of local plants for their primary health problems treatment. This paper provides the information on the traditional uses of some of the plants such as Asparagus racemosus, Acacia catechu, Albizzia lebbek, Berberis lyceum, Bryonia laciniosa, Azadirachta indica, Adhatoda vasica, Andographis peniculata, Aegle marmelos, Celastrus peniculata, Cymbopogon flexsosus, Cuscuta reflexa and Holarrheana antidysentric of Hamirpur district for the treatment of various disorders such as anthelmintic, anti-fungal, diabetic, antibacterial, cancer, diarrhea, bronchitis, tuberculosis etc. Hence the objective of the study was to document ethnobotanical knowledge primarily of notable herbs employed by the different backward people, whether tribal or rural, in the area under study. Therefore the documentation and recording of traditional knowledge associated is necessary step for the preservation of traditional knowledge about the use of local plant wealth and conservation of plant species of study area. The data would be useful for further scientific exploration.

To study the characteristics of microorganisms, it is essential to culture and growth them. Growth is the increase in cell number which can be obtained by providing proper physical and chemical conditions. Medium is a substance that provides nutrients for growth and multiplication of microorganisms. Any medium for the cultivation of bacteria and fungi must provide certain basic nutritional requirements which include a carbon, nitrogen, phosphate and various mineral nutrients. The following is the medium composition for the isolation and culturing of different biocontrol agents

Abstract

Lean season ornamentals of Shimla are observed for two years. Out of them, the flowering plants include those blooming more or less throughout the year, those having extended flowering up to early winter i.e. November, those begins to flower well before their actual flowering season i.e. spring and those which yield flowers mostly in winter. Some plants having good stalk length, keeping quality and attractive flower such as Reinwardtia indica, Asclepias curassavica and Spermadictyon suaveolens can be tried as cut flower. Some of foliage plants during spring produce beautiful flowers. Planting of these dual purpose plants will have the advantage of beautifying these temperate hills all the year round. Others are evergreen foliage plants with insignificant flower value. All the above plants belong to various utilitarian groups viz. annuals, herbaceous perennials, trees, climbers etc. and can be put into different uses. Some are potential multipurpose economic plants. This preliminary treatise reveals that there are adequate plants available within the premises of Shimla for lean season beautification. The ways to proceed further are also detailed.

INTRODUCTION

Ethno-Veterinary Medicine (EVM) or Veterinary Anthropology refers to holistic and interdisciplinary study of traditional knowledge, skills, methods, practices and folk beliefs of the people about the health care of animal husbandry and production of livestock (McCorkle, 1986). It encompasses information on diseases and their control; remedies and clinical practices for treatment and prevention; management, feeding and breeding strategies; spiritual elements; and the use of human resources that hold the information and experience (Mathias, 2004).

Notified Varieties of Different Seed Spices

INTRODUCTION

The field of virology is still not explored extensively. The use of bacterial and algal viruses till date are mostly restricted to areas like phage therapy (Sanmukh et al., 2012), whole genome sequencing, isolation and characterization of lytic enzymes (Paunikar et al., 2011), control of pathogenic multi drug resistant micro-organisms, structure elucidation through electron microscopy as well as NMR spectroscopic methods. Bioinformatics approach is also well explored for the gene annotation, sub cellular localization as well as structure prediction of various unknown as well as unclassified proteins in different viruses (Sanmukh et al., 2010). Our main concern while dealing with most viruses is limited to its classification and characterization but, we forget to reveal its true nature by exploring various budding applications which they may suffice. Moreover, very few viruses are sequenced and their genome is made available at ICTV (International Committee for Taxonomy of Viruses) which makes their information more unclear. Considering all above scenario, we can feel the urgent need for entering into a new area of “Applied Virology”. Due to various limitations with respect to funding and possible facility requirements, researchers are not yet optimizing on the applications of viruses for potential molecular biology applications as well as its industrial implementation in cost effective, time efficient and for commercial gain.

Introduction

With growing concern about climate change, environmental sustainability, soil health and food safety, conservation agriculture, organic agriculture and protected cultivation are emerging production systems being popular in India. The changing farm practices in these production systems invite many biotic stresses and demand novel approaches for their sustainable management for profitable farming. The dynamics of biotic stresses vary from system to system; therefore they require system specific approaches for sustainable management. The different agronomic/ plant protection measures practiced in above mentioned emerging systems are listed in Table 1. Protected cultivation is meant for the high value crops are grown, the management of biotic stresses is the foremost important to minimize losses. Organic farming demands more preventive measures for sustainable control of biotic stresses rather than curative measures because of complete restriction on use of harmful chemical pesticides. Conservation agriculture demands more of ecofriendly measures for biotic stress management with minimal use of pesticides. In this chapter, we discussed novel approaches for biotic stress management in emerging production systems.

The possibility of making genetic changes in the laboratory in plants, animals and microbes used in food started almost 10,000 years ago. Along with natural changes taken place during the evolution totally different species have come into existence. This has helped in increased food production with better taste and nutrition. The losses caused by biotic and abiotic stresses from fungi and bacterial pathogens have reduced.

Omics technologies based on genes and proteins involved in the nematode activities and the plant resistance responses are key factors to novel nematode management. Techniques such as next-generation sequencing of genomes and transcriptomes, RNAi, PIs, MAS, chemo-disruptive peptides and genetic transformation systems with reproducible results must be available within phytonematodes management strategies to enhance crop yields (Abd-Elgawad, 2022). Linking long-read sequencing to the use of high density genetic mapping can also support the detection and characterization of nematode-virulent genes. Progress in computational biology, bioinformatics, and analyzing the omics large-scale data can efficiently boost these techniques to offer accurate recognition of components and pathways engaged in nematode parasitism and plant response. Updated genome-editing devices will serve classical plant breeding and precisely translate how gene actions are linked to phenotypic performances. Yet, these methods must be cautiously used to avoid unwanted effects such as nematode virulence and pleiotropic impacts on qualitative and quantitative crop yields. Definite issues such as those related to the transfer of multiple disease resistance traits, gene original construct, and specificity of the virulent nematodes to the R-gene may arise in particular cases.

Horticulture has emerged as one of the potential agricultural enterprises in accelerating the growth of economy in India. It offers not only a wide range of options to the farmers for crop diversification, but also provides ample scope for sustaining large number of agro-industries which generate huge employment opportunities. Horticulture has been evolving in form of horticulture industry in our country owing to its significant role in nutritional security, poverty alleviation and employment generation. The production of fruits, vegetables and flowers has acquired much importance in recent times due to their increasing demand. India has a wide variety of climate and soil on which different horticultural crops such as fruits, vegetables, ornamentals, medicinal and aromatic plants, plantation crops and spices are cultivated successfully.

ABSTRACT

The black pepper, ‘the king of spices’ cultivation fetches foreign exchange worth millions of rupees annually, has been constrained by the quick wilt disease caused by the fungal pathogen Phytophthora capsici. Peptides with systematic variations in cationicity, hydrophobicity and amphipathicity were designed based on Chou-Fasmann parameters and sequential alterations of amino acids were tested for antifungal activity against Phytophthora. The antifungal properties of these biodegradable and ecofriendly peptide peptides were evaluated following in vitro microplate titre assay using Phytophthora zoospores. Two peptides LD1 and LD4 showed very low MIC value as compared to that of well-known antifungal peptides and standard chemical fungicides. Fluorescent staining technique using fluorescent brightener-28 and propidium iodide dyes has revealed that the site of action of the designed peptides is the cell membrane. These peptides showed very low haemolytic potential inspite of their potent antifungal activity. The in vivo evaluation of the peptides on fungi infected detached pepper leaves has demonstrated their high antifungal potential.