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An overwhelming proportion of plant material used for compounding herbal medicines and nutraceuticals is currently collected from the wild. With traditional health care systems gaining increasing popularity, the enormous amount of plant material extracted from the limited plant populations available in natural stands is a cause of justifiable alarm. The unscrupulous plunder of plant populations growing in the wild is pushing increasing number of important species to the endangered category and many to the verge of extinction. Also, unethical practices like adulteration and storage of medicinal plants under unacceptable conditions are rampant. All this is happening, when the consumer is becoming increasingly quality conscious and is demanding traceability of source material used for formulations and adherence to good practices for the entire chain of manufacturing process for herbal drugs and plant-based nutraceuticals. Clearly, solution to this problem lies in replacing the ‘collection from the wild ’ paradigm to ‘production by cultivation ’. An essential prerequisite for meeting this objective is a well thought out research agenda which not only tackles productivity enhancement goals for the targeted plant species but also addresses the validity of the entrenched notion among healers who use the traditional medical systems, that only material collected from the wild produces the desired results. The reason advanced in support of this argument is that phytomedicines are derived from plant populations growing in the wild under organic conditions and under their natural rhizosphere and rhizoplane. The associated soil-microbial complexes, and a variety of endophytic microorganisms, may be the initiators or modifiers of the production and expression of medicinal properties.

It also so happens that a majority of medicinal plants are shade loving, C4 types and will necessarily require their species-specific ambient environment for elicitation of the medicinally relevant moities.

ABSTRACT

Plants are the basic requirement upon which all other living organisms depend. Plant resources have been used over the decades for human welfare in promotion of health as medicine, flavour and fragrance. Natural essential oils derived from plants are used as cosmetics, perfumes and flavors in foods and beverages. Development of processsing industries are directly linked to specific needs, available resources and intervention of technological capabilities. Resourgence of public interest in herbal products has created a huge market for plant based products which not only satisfies human needs, but also provides quality and safety insurance. Himalaya range in Southeast Asia is a hub of medicinal and aroma bearing plants globally recognized for their chemical constituents as well as endemic and threatened nature. These plant resources has high demand in international market for development of value added products, for isolation of commercial important compounds used in drug discovery programmes, preparation and formulation for development of new nutraceutical products and semi-synthetic derivatives for medicines. Aromatic plants are valued for their aromas, tastes and their applications in treatment of various illness, and mostly prefered in cosmatics, perfumes, confectionery food items and medicines. More than 3000 aroma bearing plants have been characterized world wide, of which, 116 aromatic plants are recorded from Indian Himalayas. Lavender, Geranium, Lemongrass and other aroma bearing plants are the main primary ingredient for many new and existing herbal products. An attempt has been made in this communication to present an account of different aromatic wealth of Himalaya, extraction techniques and their major chemical constituents, which will be helpful to pharmaceutical industries in development of value added products in time to come.

ABSTRACT

Plant tissue culture is an important and efficient technique for the production of desired plant products with different and improved variety in aseptic conditions. Plant tissue culture methods have a wide scope for the creation, conservation, and utilization of genetic variability for the genetic improvement of field, fruit, vegetable, forest crops, medicinal and aromatic plants. The main objective of this communication were to collects the information and techniques of tissue culture to genetic improvement of the medicinal plants. Tissue culture technique in combination with molecular techniques has been successively used to incorporate specific traits through gene transfer. Modified varieties of medicinal plants is mostly required, as some compounds like alkaloids, terpenoids, steroids, saponins, phenolics, flavanoids, and amino acids etc are extracted from these plants. From the ancient time up to 20th century, most of the human-being are dependent on the medicinal plant for basic healthcare needs and improving such a crop is the most important for this billionaire world. As far conventional methods of genetic improvements the tissue culture techniques takes cheap and lesser time required for traits specific improvements in the MAPs.

ABSTRACT

The use of engineered nano-materials has been increased as a result of their positive impact on many sectors of the economy including agriculture. Silver nanoparticles (AgNPs) are now used to enhance seed germination, plant growth and as anti-microbial agents to control plant diseases. This study investigated the synthesis of silver nanoparticles using the aqueous solution of three medicinally important plants (such as Spondius mombin L., Stachytarpheta jamaicensis (L.) Vahl and Syzygium samarangense (Blume) Merr & Perry leaf extract in room temperature (350C). AgNPs present in the leaf confirmed by colour change, UV-VIS spectrum analysis, average size of AgNPs confirmed by PAS. FTIR measurement were carried out to identify the other possible biomolecule groups such as alkenes, esters, phenols, alcohols and aromatic. Leaf extract AgNPs were shown significantly higher antimicrobial activities against four species of bacteria. This findings suggest that the seed germination percentage, relative seed germination rate, relative shoot and root growth and germination index of the tested plant depends upon concentrate gradient of AgNPs.

ABSTRACT

Cultivation of mat grass (Cyperus pangorei Rottb.) is valuable and expensive products can plays an important role in rural areas such as Sabong and Pingla in Paschim Medinipur of West Bengal in India. Mat grass plays an act to fill the empty employment field to the resource of poor farming community to support and secured their livelihoods and maintain the economical balance of their area. This grass usually cultivated by the poor and marginal farmers. The mat sticks are used to make various household utility items, such as floor and bed mats and other attractive goods like bags, purse, TV covers. Now a days the products prepared from this grass is exported to different countries. Mat sticks are attractive durable and is eco-friendly substances which easily gets decomposed by organisms, does not create any environmental hazards and gets more profit as compared to growing rice per annum. Even an old man or woman in rural areas can earn a net income of about Rs.120 to Rs150 per day. Major focus of the study is to establish the scientific principles behind processing, separation of clum strand, texture and strength properties of the mat fibers and increase mat production which leads to improve the economic condition of rural people.

ABSTRACT

Evolution theory postulates that the collision between the Indian and the Eurasian plates in early Eocene and Miocene epoch leads to formation of the Himalayas, and currently recognized across the globe as repository of life. Some species of this regions is categorized under endangered and endemic category. Rhododendrons placed under family Ericaceae is an important genus of flowering plants. It is one of the multiferous keystone member of plants, mostly endemic to Southeast Asia. Wide distribution of rhododendrons concentrated in Himalayas. The Eastern Himalaya, especially Arunachal Pradesh and Sikkim states of India is the main centre of species distribution and provide wide range of habitat for ecological studies. Several species exhibits many nutritional, medicinal and aromatic properties as evident from tribal knowledge and published research sciences. Different parts of plant such as flowers and leaves used in folklore herbal medicine and for preparation of local wines. Chemically, rhododendrons are repository of several bioactive molecules in the form of taraxerol, hyperoside, betulinic acid, quercitin, arbutin, rutin, coumaric acid, and several other fine molecules in minor quantities. In the present communication, Indian rhododendrons species diversity of different regions are revised and updated the current list for India. Besides, its value addition and future prospective is also presented for Indian economy. The checklist, chemistry, traditional knowledge, ecological requirement, keystone taxa, potential value added products for future, threat and future perspectives of rhododendrons for conservation is also discussed and presented in different subheads. First time checklist of all Indian rhododendrons are prepared and provided here in this communication.

ABSTRACT

Eulaliopsis binata (Retz.) C.E. Hubb. is a valuable grass growing in Midnapore district of West Bengal known for their commercial purposes. Livelihood of and many of peoples are depends on the production of this grass. Besides this we try to present their phytochemical constituents mainly alkaloids, flavonoids and phenolic compounds for medicinal study. In this investigation, we studied the morpho-taxonomy and anatomical characterization as well as phytochemical screening of different chemicals. Besides this we make an idea about presence of sugar molecules by HPLC study and chemical bonding properties with the help of FTIR. The detailed study gives an idea about E. binata and its medicinal properties.

ABSTRACT

Green revolution provided sufficient amount of foods but the way resources were utilized causes the degradation of natural resources in various aspect. The adverse effect on the ecological system can be visualized in the form of various diseases in human and loss of biodiversity. The population burst is the root cause of imbalanced ecological system because agricultural land decreases drastically. Therefore we must aim to develop and improve the agri- economic growth of farmers by introducing elite variety of Mucuna pruriens (L.) DC. M. pruriens, a member of Fabaceae family, is an adaptogen and known for its tonifying, strengthening and all around beneficial properties from every part of the plant. M. pruriens has range of medicinal applications, because of unusual amino-acid L-DOPA (L3, 4dihydroxyphenylalanine) a precursor of neurotransmitter dopamine (Anti Parkinson’s) and involved in various secondary metabolite pathways. It acts like aphrodisiac, anti-snake venom, anti-depressant, antidiabetic, anti-microbial. It has a nutritional quality comparable to soyabeans. M. pruriens exhibits a tolerance for various kinds of environmental stresses such as drought, low soil fertility, and high soil acidity. M. pruriens suppresses weeds through indeterminate growth and large leaves reduce the amount of light for weeds. It shows allelopathy and effective in lowering nematodes. M. pruriens is used as forage, fallow, green manure prevents the soil erosion. It has a longer time span to be used in rotations for management of various pathogens.

ABSTRACT

Commercially valuable plants are one of the main sources of economy. Growth, development and yield of these plants are severely affected by various environmental factors, which depend on dynamic fluctuations in different parameters. To counteract these responses, plants try to adapt evolutionarily but one form of adaptation is never going to suffice the simultaneous attack of multiple stresses. Crossing and plant breeding approaches came to practice, which is quite beneficial but requires lot of screening and is manpower consuming. In recent era new approaches have been developed combining recombinant DNA technology with tissue culture techniques to obtain improved varieties with specific desired traits. This method had an edge over other technologies but it still carries lot of complications. It is evident that genes do not act alone but act cooperatively and remain in continuous influence of other factors. So to target a particular gene for improved variety development requires a lot of detailed micro-details. In this communication, an attempt have been made to discuss mainly how transcription factors (TFs) have been targeted for this purpose. TFs in many occasions act as master regulators or central players of a particular pathway. This is attained by their ability to interact with multiple cis-elements present in the promoters of downstream stress or metabolism responsive genes. This behavior sometime leads to occurrence of some unwanted traits, which should be kept in mind to develop transgenics. Here, we focused mainly on important TF families such as ERF, WRKY, NAC and bHLH. We have been an attempt to analyzed how these TFs influence growth, yield and stress response of commercially valuable plants such as rice, wheat, tomato and cotton. This section will help to understand the genetic manipulation of plants can be targeted for improved variety development.

ABSTRACT

The people meet their need by taking pulses as their daily meal and therefore, the pulse play a significant role as food supplements. Pulses are called ‘Nutrient Powerhouse’ and are edible food of plants in the legume family. It contains high protein, fibers, various vitamins and amino acids. Starch is the main form in which plants store carbon. It occurs as semi crystalline granules composed of two polymers of glucose called amylose and amylopectin, depending on the plant organ. Starch granules are characterized by internal growth rings. Starch grain is a very useful taxonomic tool in plant systematic. In this investigation we atudied the morphological variations of starch grains in seeds. These starch grain contain the composition of carbon to form different structural diversity of seeds like simple i.e. two types: concentric and eccentric, semi-compound and compound. Polarized microscopy (PM) was used for better micro-morphological (Maltase cross) study. These micro-characters are carried out by UPGMA and construction dendrogram for visual appreciations of taxonomic relationship.

ABSTRACT

Soil salinity is one of the most serious environmental issues that limit agricultural productivity. Most of the food crops belong to glycophytes and hence, they are not able to withstand salinity stress. Salt stress significantly affects the various morphological, biochemical and physiological processes of the plant which leads to poor plant growth. Therefore, there is an emergent need to find out mitigation strategies to cope with the deleterious impacts of salt stress. Plant breeding techniques, efficient resource management and genetic engineering have been employed to cope with salt stress but these were of limited success as salt tolerant trait is complex both genetically and physiologically. Therefore, using plant growth promoting bacteria seems to be a cost effective and sustainable approach for salinity stress management. Hence plant growth promoting bacteria could play a significant role in alleviating salt stress. This chapter endeavor to provide an overview of the various important mechanisms such as facilitation of nutrient uptake, production of phytohormones, ACC deaminase production and siderophore production employed by the plant growth promoting bacteria to enhance the plant growth under salinity stress.

ABSTRACT

The deeply rooted coastal grass Spinifex littoreus (Burm.f.) Merr. is a potent soil binder having capability to form a strong and stable dunes. However detailed features of this species has not been thoroughly studied till date. In this communication, salient morphological features along with different crucial micro-morphological peculiarities have been discussed. The comparative analysis of gradual degradation of population of Spinifex littoreus and native dune vegetation of the coastal belt in East Medinipur of West Bengal and adjacent Balasore district of Odisha are provided, along with different biodiversity indices. Plants were characterized as stout, dioecious, stoloniferous grass upto 90 cm tall, with rigid and sharp spiny leaves. Spikelets in racemes subtended by large bract-like spatheoles which are fascicled into large capitate spiny structures 30-37 cm in diameter. S. littoreus is fairly common along sandy shores and dunes. Plants are useful as a sand binder in unstable coastal dunes ecosystem.

ABSTRACT

Artemisia annua L. is known for the content of anti-malarial drug, artemisinin. It is widely distributed in subtropical, temperate and subtropical zones worldwide and traditionally used for the treatment of various ailments. Artemisinin has been the frontline treatment since the late 1990s and saved countless lives, especially among the world’s poorest children. The major chemical constituents of Artemisia annua L. are sesquiterpenoids and some phenolics. The essential oil of Artemisia contains artemisia ketone, camphor, caryophyllene oxide, 1,8- cineole and -pinene, and these essential oils constituents are known for their medicinal properties.Artemisinin was discovered in 1971 bya Chinese Medicinal Chemist Youyou Tu (Nobel Prize in 2015). As the demand for artemisinin remains high worldwide, exploring the chemical and the genetic variation especially knowledge, about its mechanisms for high-yielding would be more important. The plant extract is also known for other biological activities such as anti- hypertensive, anti-microbial, immuniosuppresive, and antiparasitic activities. To increase the production of artemisinin and to investigate the effect of climate change on artemisinin content will be the major research area in future from Artemisia genus. Exploring the alternative resources such as microbial production and semisynthetic approaches from other easily available natural molecules would be of high demand. Some other molecules from Artemisia should also be focused for the future development of man kind and in drug discovery science. An overview of present status of medicinal applications, phytochemistry, and future perspectives for the research possibilities on the active ingredient Artemisinin from A. annua is discussed in this chapter.

ABSTRACT

Due to the exorbitant price of the popular gelling agent agar, Plant Tissue Culture (PTC) has gradually become a costly technique in recent times. So, alternative sources of agar needs to be popularized which at one hand should be able to meet all the positive properties of agar and on the other hand be cost effective as well. The seed husk obtained from Plantago ovata Forsk. is such an alternative which was successfully used as a gelling agent for media preparation in PTC experiments for in-vitro shoot and root development in Nicotiana tabacum L. and Ocimum gratissimum L. The nodal explants were grown in MS basal medium (MSo) supplemented with 3% sucrose and were gelled with progressive concentrations of isabgol husk to ascertain the optimum concentration for better survival and growth response, while 0.8% agar acted as a negative control. The response of the explants on media gelled with husk was compared to that of media gelled with 0.8% agar. It was inferred that 0.3% husk in the medium was optimum for best growth response which was comparable to that of agar. Consequently, the estimated cost of a liter of MSo reduced by almost Rs.61.15/- (compared to Himedia’s Agar) to Rs.177.57/- (compared to Sigma’s Agar) after using isabgol seed husk as a gelling agent in media preparation.

ABSTRACT

Agrotechnology of banana has been introduced for the first time by CSIR- Indian Institute of Integrative Medicine Jammu for commercial cultivation of banana in Himalayan Shivalik range. The aim of the present investigation is to study the potential of Tissue Culture (TC) banana production in a diverse environment of Shivalik range, to make the state self-sufficient in banana production, employment generation and generate revenue for the farmers and to studies on the post harvest handling and marketing of TC banana. Initially, the sapling of this high quality tissue culture variety {BHIM Grand naine (G-9)} Banana was brought from Agro-division of Cadilla Pharmaceuticals Limited Ahmedabad, Gujarat. The field experimental trial was conducted during 20016- 2017 at CSIR-IIIM Research Station, Chatha Farm, Jammu in which four treatment combination arranged in Randomized Complete Block Design (RCBD). Significant differences were observed among the treatments for all the parameters studied. Among the date of planting, banana planted at 10th August reported significantly highest pseudostem height, pseudostem girth, more average number of leaves/plant, first flower emergence (days), first finger ripening (days), more number of finger per hand, more number of hand/ branch, higher weight of finger/hand (kg), higher weight of single finger (g), more finger diameter (cm), higher length of finger (cm), more weight of finger/ bunch (kg), more percentage of plant harvested at a time, higher yield (64.22 tonnes/ha), gross return (Rs. 12,84,400/ha), net return (Rs. 9,58,730.5/ha) and B:C ratio (3.94) as compared to other treatments. On an average the yield of per plant was 20-30 kg and 50- 64 tonnes per hectare. In terms of economy as per market analysis, price of banana in Jammu is approximately Rs. 20 per kg. Thus, on an average it gave Rs. 250-300 per banana plant. On the basis of market demand, approximately Rs 6.17- 9.50 lakh net return can be obtained by cultivation of one hectare of land which is alternative business for the farmers of the Jammu and Kashmir. This may leads to revolution of banana cultivation in Shivalik range of Himalaya.

ABSTRACT

In the present investigation on three different plant species from pollution and control area were carried act for biochemical analysis such as total chlorophyll content, leaf extract pH, relative water content and ascorbic acid test. The results indicate accordance to order of tolerance index of plant species in air pollution are Macrosolen cochinchinensis, Loranthus parasiticus and Viscum album. The changes in pollution tolerance index are biochemically induced due to the surrounding environmental conditions. Thus, it is observed that Macrosolen cochinchinensis is highly tolerant species among the selected three species. Thus, in future this plant species may play an important role in pollution indicator. APTI analysis show the pollution tolerating decreasing order for there species can be Macrosolen cochinchinensis> Loranthus parasiticus>Viscum album and as ascorbic acid result indicate that Macrosolen cochinchinensis show mostly defence mechanism and highly tolerance level against different kind of pollutant. Besides these three hemiparasite plant species have some valuable ethnomedicinal important. In India tribal people used leaf and stem bark in curing abortion, miscarriage, vaginal bleeding during pregnancy, treat circulatory and respiratory system problems.

ABSTRACT

Dysoxylum binectariferum (Roxb.) Hook. is an evergreen tree, indigenous to India and widely distributed in different parts of India. Primarily, this plant is used in the timber market for the preparation of furniture and other wooden goods. Apart from utility in timber market, it has tremendous medicinal properties because of its unique secondary metabolites. Its hydroalcoholic extracts possess a wide range of pharmacological activities including immunomodulatory, anti-inflammatory, anti-cancer, anti-leishmanial, etc. The chromone alkaloid rohitukine, the major constituent of the plant, possess anti-fertility, anti-leishmanial, anti-adipogenic, anti-ulcerogenic, anti-cancer and immunomodulatory activities. The most extensively studied and advanced therapeutic property of this chromone alkaloid is its cyclin-dependent kinase inhibition activity, which has a direct link with its anti-cancer effect. Inspired by this natural product, two clinical candidates (flavopiridol and riviciclib) are discovered and studied in human cancer patients for treating various types of cancer. Another preclinical lead IIIM-290, which is directly derived from rohitukine via synthetic modification, has demonstrated promising oral efficacy in animal models of various cancer types. The high-value medicinal applications of this plant have necessitated the need of bringing this tree under captive cultivation. The present chapter discusses the biology pharmacological and secondary metabolites present in Dysoxylum binectariferum. The preclinical and clinical leads derived or inspired from rohitukine are also discussed in this communication.

ABSTRACT

Bunium persicum (Boiss.) Fedtsch., (Family: Apiaceae) is an important plant of temperate regions and its seeds are used as carminative substance in various food recipes. The seeds of the species act as a good source of economy to the hilly and mountain people. As the species is restricted in its habitat, therefore, it requires mass cultivation practices. Many attempts are already made to cultivate species near to its natural habitat. In recent years some efforts have been done to domesticate this plant in certain areas of India like Shong area in Kinnaur district of Himachal Pradesh, India, but the cultivation remained very limited. Therefore, in addition to its collection from the wild sources the species needs more scientific intervention in agriculture sector. This species has typical Palaeo-Mediterranean distribution. In India the species is distributed in wild only in North West Himalaya (J&K, HP and UK) which makes its ocurrence rarity in the country. The species restricted its distribution between 1850-3100 meters above mean sea level. Phylogenetically the species is near to Carum carvi L., therefore, C. carvi is the potential adulterant of B. persicum.

ABSTRACT

Magnolia campbelii Hook.f. & Thomson is a deciduous tree species belonging to Magnoliaceae and is highly valued for its ornamental uses. The species is threatened by habitat degradation as well as its utility as timber. It is distributed in the eastern Himalaya, southern Myanmar, and Yunnan province of China. However, its exact distribution in the eastern Himalaya is unknown owing to rough terrain and remote location of the Himalayas. We modelled the environmental niche and delineated the potential habitats and distribution areas of the species in this study using ENVIREM dataset and Maxent software. Thermicity Index and Topographic Wetness were the critical environmental variables determining the species distribution in the eastern Himalaya as revealed by Jackknife analysis. Geographical projection of the modelled environmental niche indicates that most suitable areas for its distribution are the western part of Arunachal Pradesh, few pockets in Sikkim, and the central part of Bhutan. The results of the study have implications for in situ conservation of the species as well as identifying suitable areas for reintroduction.

ABSTRACT

Tinospora cordifolia (Willd.) Miers ex Hook.f. & Thoms. is a versatile liana rich in various active compounds having diverse chemical structures. Limited research has been done on the biological properties and the probable medicinal applications of the individual isolated molecules actually responsible for the medicinal nature of the plant. Recent advances in the discovery of active constituents from T. cordifolia and their biological impact in disease cure has led to an increase interest on this plant across the globe. The therapeutic virtue of giloy used in traditional and Indian System of Medicine (ISM) has been well established through advance testing and evaluation, both pre-clinical and clinical trials in different diseased conditions. These studies make this indigenous drug plant a novel candidate for bio-prospection and drug development for the treatment of diseases such as cancer, ulcers, liver disorders, heart diseases, diabetes and post-menopausal syndrome where sufficient treatments are still not available. Over the past few decades, a noteworthy progress has been achieved in cancer prevention and treatment, and still there remains a need for effective treatment program. One of the best possible approaches with immense potential is chemoprevention, which involves the use of natural, synthetic or biological molecules to suppress, reverse or prevent cancer or the progression of cancerous cells. It is most likely that many agents present in specific herbs, herbal products and natural dietary factors will have an effect throughout the process of carcinogenesis. Thus, a detailed chemical investigation with the aim of isolating novel molecules, their structure elucidation, quantification, validation and pharmacological evaluation in terms of its anticancer potential was carried out on T. cordifolia. The present study was designed to isolate and identify new promising anti-cancer candidates from the aqueous alcoholic extract of T. cordifolia using bioassay-guided fractionation.