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The exploration of facts about healthy and nutritious food has increased over the years. The advent of advance scientific tools has elucidated facts regarding the role of diet or nutrients in regulating gene expression (Sales et al., 2014). Various studies based on molecular interactions in food has revealed that many dietary components such as proteins, carbohydrates, fats, minerals, vitamins and phyto-constituents has the tendency to regulate gene expression.

4.1. Introduction
The food is the main factor known to affect the health of an individual. By interference in the protein synthesis, function and metabolites and alteration in genome expression, the healthy condition must be altered to diseased condition. Side by side we can say that the dietary component directly or indirectly modulates the molecular fingerprint and understanding of which is dealt with nutrigenomics. So, by modulation of a particular gene for a specific disease by nutrition, we can prevent the specific disease and promote the health of a population. Consuming fruits and vegetables on a regular basis has been linked to better health outcomes and diminution of several chronic or life style linked diseases.
Concept of Nutritional Genomics
After completion of human genome sequencing, the unique term “Nutritional genomics” tends to replace the old term “Nutrient-gene interaction”. It has been stated that so many polymorphism can influence protein structure and function. There are several regulatory sites where nutrients can impact the flow of genetic information. The two components of the nutritional genomics field are nutrigenomics, which studies how dietary components interact with the genome to regulate changes in proteins and other metabolism, and nutrigenetics, which determines how dietary components affect genetic variations. (Farhud et al., 2010). Nutrients are as environmental influences can interact with genetic material. It is commonly known that DNA metabolism and repair require a range of dietary elements that serve as cofactors or substrates in metabolic pathways. However, little is known about how cofactors and/or micronutrient excess or deficiency affect the accuracy of DNA replication and repair (Caroline and Fenech, 2008). The response to a particular nutrient, which is determined by the individual genotype, must also be taken into consideration, even though the nutrients can influence the development of a particular phenotypic.

Abstract

Nutrition and genetics both have very crucial role in human health and in development of chronic diseases such as cancer, osteoporosis, diabetes and cardiovascular diseases. Difference between individuals in response to dietary intervention is a well-known phenomenon in nutrition research (Ordovas, 2008).The effect of dietary changes on phenotypes such as blood cholesterol, body weight and blood pressure can differ significantly between individuals (Ordovas et al., 2007). Various bioprocess i.e. ingestion, absorption, digestion, transport, metabolism and excretion of nutrients and food bioactive components in human body is carried out through proteins such as enzymes, receptors, transporters, ion channels and hormones. Genetic variation leads to altered production and affect functional properties of these proteins. Furthermore nutritional efficacy is also different among different individuals which might be affected by nutritional status. Thus diet and gene expression are interrelated and have a mutual effect on physiology of human body.

Introduction

The dairy farmers mostly keep cows (indigenous and crossbred) along with few buffaloes for milk production. However, the genetic potential of the dairy animals is exploited fully, when they are fed with well balanced ration. Further, as feed cost is around 75% of the total cost of production, the success of the dairy farming depends not only on the quality of the ration, but also on the economy of the ration. Therefore, knowledge on the feeds and feeding of dairy animals is highly essential.

What is Feed?

Feed may be defined as supplementation of nutrients for development, maintenance and operation of day to day activities by animals.

Mice are omnivores and can be fed with commercial feeds like pellets or fed with mixed feed which is locally available cereal, pulses and grains.

However, nutritionally requirement of mice

Carbohydrates - 45-55%

Protein - 16- 24%

Fibre - 4-5%

Fat - 4-6%

Supplement vitamin and minerals.

Rabbits are produced for meat, wool and research and as pets or for a hobby. Meat production is the most important commercial aspect of rabbitry. The domestic rabbit is primarily herbivorous and will consume most types of grains, oil seed cakes, brans, greens and hay. Diets provided, whether home grown or commercially prepared, consist almost entirely of ingredients from plant sources. Although, the producers / farmers mostly rely on home grown feeds, a good portion of the rabbit feed presently used is commercial pelleted feed. Since the rabbit can utilize a certain amount of forage, it has a place in food production by making use of some non-competitive feeds.

Nutrition and feeding management are crucial components in the seed rearing phase of aquaculture, significantly impacting the growth, survival, and overall health of juvenile fish and shellfish. In the Indian aquaculture industry, which primarily cultivates Indian Major Carps (IMCs), catfish, tilapia, pangasius, shrimp, and freshwater prawns, implementing species-specific and stage-wise feeding practices is essential. During the early developmental stages such as larvae, fry, and fingerlings, the nutritional demands are high due to rapid growth and the development of internal organs. To meet these needs, live feeds like infusoria, rotifers, Moina, Daphnia, and Artemia nauplii are commonly used as they are highly digestible, appropriately sized, and capable of stimulating a natural feeding response. As larvae mature into fry and fingerlings, they are gradually transitioned to formulated micro-diets or crumble feeds that typically contain 30–40% protein and 6–10% lipids. For shellfish species such as shrimp post-larvae and Macrobrachium, specialized starter diets enriched with immune boosters and probiotics are utilized to enhance survival and health. Feeding is typically carried out 3–5 times daily during the nursery phase, with rations adjusted according to biomass and water temperature. Care is taken to avoid both overfeeding, which can lead to water pollution, and underfeeding, which is managed through regular sampling and observation. In extensive systems like ponds and hapas, natural food availability is increased through the application of fertilizers and manures to stimulate plankton production. Overall, maintaining a balanced diet and adopting scientific feeding strategies are vital for producing healthy, fast-growing, and disease-resistant juveniles, thereby improving the economic efficiency and sustainability of aquaculture in India.

(I) Digestion of Feed

In feed, carbohydrate is the main nutritive substance which is in the form of insoluble large particles. For intestinal absorption by blood and lymph, it is necessary to be digested as small and simple particles. For example, carbohydrate found in the form of starch or glycogen is not absorbed as such and is only absorbed when it is converted into the simple soluble monosaccharide form. Similarly, proteins present in the feed are also not absorbed untill they are digested into simple forms of aminoacids (Fig. 27, Fig. 28, Fig. 29, Fig. 30). Piglets are exceptions, as the newly born piglets obtain colostrum from their mother. Colostrum is rich in gamma immunoglobulins. This immunoglobulin is abosrbed as such without digestion by pinocytosis. It gives immunity to save the new piglets from many diseases. For this reason, colostrum suckling is necessary for new piglets. The process of pinocytosis occur only few days after birth and it  stops after some time. Fat occurs in the form of triglycerides in the feed. For absorption of the fat, it is necessary to be converted into its simple forms such as glycerol, free fatty acids or in the form of monoglycerides.

Carbohydrates exhibit protein sparing action, because proteins are mainly required for tissue – building, that is, general wear and tear in the body. If there is any emergency, say animal is deficient in calories of the diet, then it will use adipose and protein tissues. It is said that proteins and fats are burnt (or oxidized) in the flame of carbohydrate. It means that certain intermediary compounds of glucose oxidation through Krebs cycle, are absolutely necessary for oxidation of proteins and fats. Again, in any emergency, if glucose level of the body goes down, fats and proteins are taken over and they get metabolized faster than the body can take care of the intermediate products. However, ketone bodies – acetone, acetoacetate β – hydroxy butyrate appear in urine. In addition to above, monosaccharides are very important (vital)m structural components of many compounds which regulate metabolism. Among these are DNA, RNA for transfer of genetic information of the cell, which contain ribose and deoxy ribose sugars. Glucuronic acid occurs in the liver and this combines with toxic chemicals and bacterial by – products and hence, acts as detoxifying agent. Hyaluronic acid (a disaccharide) forms matrix of connective tissue. Heparin, a mucopolysaccharide, is very important anti – coagulant. Chondroitin sulfates are present in cartilage, bone, skin and tendon. Glycosides are widely distributed throughout the plant kingdom and a number of them have been used as drugs for animals. 
Lipids: these are the most concentrated form of the stored energy in animal kingdom, because they provide 2.25 times per unit more energy than carbohydrate. They provide insulation for vital organs protecting them from mechanical shock and also maintain body temperature. The cell membranes have phospholipids, for example – Erythrocytes. Prostaglandins exhibit hormonal activities. Essential fatty acids – linoleic, linolenic and arachidonic acids show deficiency symptoms in their absence. Lipid also delays hunger, because it requires longer time to pass through stomach than carbohydrate or protein. They also help in lubrication.

Introduction

Nutrition is the study of how food and nutrients affect the body’s health and well-being. It encompasses all aspects of food, from the food production, processing, consumption and digestion by the body. A balanced diet included all the essential nutrients such as carbohydrates, protein, fat, vitamins and minerals for maintaining good health. The recommended daily intake of nutrients varies depending on the age, gender and physical activity of a person. The nutritional need also varies depending on the lifestyle and medical condition of a person. The nutrition facts are based on recommended daily intakes for various nutrients, which vary based on factors such as age, sex, and physical activity. These facts are given on food packaging or label, providing important information on the serving size, calories, macronutrients and micronutrients, and other ingredients.

Nutrition labels are mainly intended to guide the consumer in food selection. Nutrition information provided on labels must be true and should not mislead consumers. Nutrition labelling regulations are provided by countries to help manufacturers. The Codex guidelines on Nutrition Labelling provide guidance to member countries to develop or update their national regulations and to encourage harmonization of national standards with international standards. According to it, no food should be described or presented in false, misleading or deceptive way. Nutrition labelling cannot solve nutrition problems, instead it is one of the elements of nutrition policy.

11.1. Nutrition Facts

Nutrition Facts means the nutritional information required for most of the packaged food in many countries giving the energy value and nutrients contained in the food. Nutrition facts labels are required by regulation similar to other types of food labels. It provides detailed information on the nutrient content of the food such as the amount of fat, sugar, sodium and dietary fiber.

Fruits and vegetables are the major constituents of daily diet. They provide not only body building elements but also energy yielding food for the wellbeing of humans. Main object of the nutrition garden is to make availability of the requisite quantity of fresh fruits and vegetables to family members. It is generally raised in the backyard of home. It is also called as a home garden or kitchen garden. Most of the day to day cultural operations are performed by family members. Working in the nutrition garden during their spare time is a pleasure, an inspiration, a means of recreation to all the family members. The foremost advantage of nutrition garden is to daily supply fresh fruits and vegetables as per the needs of family members. Nutrition garden has an important role in enhancing national food security and dietary diversity to combat malnutrition.

Points to be considered, while the planning and layout of nutrition garden

The term essential element was proposed by Arnon and Stout (1939).They concluded three criteria must be met for an element to be considered essential. These criteria are:

1.A plant must be unable to complete its life cycle in the absence of the mineral element.

2.The function of the element must not be replaceable by another mineral element.

3.The element must be directly involved in plant metabolism.

The number of elements considered essential for the growth of higher plants now varies from 16 to 20 or more, depending upon the definition of essentiality. Other scientists such as Nicholas (1961) believed that an element should be considered essential if its addition enhances plant growth even though it merely substitutes for one of the 16 elements that Arnon declares to be essential. For example, sodium can substitute in plant nutrition for some potassium and vanadium for some molybdenum, Nicholas would consider both sodium and vanadium as essential, but Arnon would not. On the basis of the criteria used, Arnon specifies 16 elements and Nicholas 20 elements as being essential for the growth of higher plants. Three other debated nutrients are nickel (urea transformations), cobalt (N₂ fixation) and silicon. These criteria are important guidelines for plant nutrition but exclude beneficial mineral elements. Beneficial elements are those that can compensate for toxic effects of other elements or may replace mineral nutrients in some other less specific function such as the maintenance of osmotic pressure. The omission of beneficial nutrients in commercial production could mean that plants are not being grown to their optimum genetic potential but are merely produced at a subsistence level.

Nutrient

Definition: Those elements are required for plant metabolism in more or less quantity, is known as nutrients. Plants have 30 types of elements but 17 elements out of 30 are essential for plant growth and development. These elements are called Essential nutrients.

Criteria of essentiality of plant nutrients theory: It is given by Arnon and Stout (1939). This theory stated that plant can not be completed their life cycle without the deficiency or complete absence of a single nutrient among the essential nutrients

Low of minimum: It is given by Justus Von Leibing (1840). According to this low, the growth of the plants is limited by the plant ntruient present in smaller quantity, all other being in adequate amounts. This has been re–stated as Barrel concept.

Allen and Arnon (1955) laid out following criteria for categorising nutrients essentiality to plants:

1. Complete or partial lack of the element in question must make normal plant growth impossible
2. Deficiency symptoms must be reversibly by the addition of the elements in question
3. The element must play specific role in the plant metabolic symptom

A. Answer the following questions

1.    What is the DM consumption in adult guinea pig? Ans. An adult animal consumes @ 7-8% of body weight.

2.    Mention daily average feed consumption of guinea pigs?

Ans: Growing Guinea pigs: 20-30g, Adult maintenance: 30-50g, Pregnant and lactating: 40-60g.

3.    Write down the peculiarities of digestive system of guinea pig.

Ans. The entire stomach of guinea pig is lined with glandular epithelium and gastric mucosa does not have a keratinized portion.

4.    Why guinea pig cannot synthesize vitamin C?

Nutrients

The nutrients are chemical elements which are absorbed by the plants in more or less quantity to transform light energy into chemical energy and to keep up plant metabolism for the synthesis of organic materials. These materials constitute among other things, foods for humans and animals and a range of raw materials for various industrial uses. Feeding of plants with nutrients is termed as nutrition. Successful growth and production of the plants in general requires a proper supply of the 17 elements. These elements are regarded as essential to life in higher plants.

4.1. Digestive System of Rabbit

The digestive system of rabbit includes:
    A.    The digestive tract 
    B.    Its associated glands
The digestive system breaks down the complex molecules into the simpler one so that it is readily absorbed from gastro intestinal mucosa.
 
A. The digestive tract (gastro intestinal tract/ alimentary canal)

The digestive tract is essentially a tubular structure for the ingestion and digestion of food. In an adult (4 to 4.5 kg) or semi-adult (2.5 to 3 kg) rabbit the total length of the alimentary canal is 4.5 to 5 m. It commences from mouth and terminates in anus. G.I.T is divided into following parts:

Food security is achieved when adequate food is available and accessible and satisfactorily used and utilized by all individuals to live a healthy and active life. But food and nutrition security is achieved when people have physical, economic and social access to food of sufficient quantity in terms of variety, diversity, nutrient content and safety to meet their dietary needs and food preferences for a healthy and active life coupled with sanitary environment, adequate health and education care. In order to achieve food and nutrition security there is a need for transitioning toward nutrition sensitive food system.

In Uttarakhand, majority of the population lives in rural areas and primarily depends on agriculture for their livelihood. In Uttarakhand, only 14.02% area is under cultivation. Out of total 7.66 lakh hectare cultivated area in the state, 4.21 lakh hectare area is rainfed i.e. more than 50 % of the cultivable area is rainfed. The average land holding is 0.68 ha that too is divided into many patches. Rainfed farming in Uttarakhand gets affected by frequent drought like situations and excessive rainfall. Food and nutritional security of people living in hill region depends more on favorable climatic conditions as agriculture and livestock farming are the main occupations. Prevailing low level of soil fertility and increased incidences of pest and disease occurrences has adversely affected the crop production and productivity in hills. Low agricultural productivity has emerged out as an important push factor for out migration of people from hill areas. Traditional hill agriculture is practiced on small and fragmented terraced fields with lack of irrigation facilities, which are uneconomical. Women constitute the main workforce in agriculture as large section of able bodied men have migrated from hills in search of livelihood. They also take care of the cattle, collect fuel wood and fodder from forests, often situated at considerable distance from the villages involving four to five hours of walking both ways, and do all household chores. Their life is an unending drudgery of hard work.

As a large population in the hill region of Uttarakhand depends on agriculture, the problem of malnutrition can be better addressed through nutrition sensitive agricultural interventions specifically designed for women in hills. Local nutritional needs can be met out locally by reviving traditional practices along with amalgamation of science and technology knowledge. Focusing on nutrition helps to build the resilience of rural households. Nutrition sensitive approach was promoted at local level with active participation of women who are mainly responsible for food security of the households.