eChapters

ABSTRACT

Green revolution provided sufficient amount of foods but the way resources were utilized causes the degradation of natural resources in various aspect. The adverse effect on the ecological system can be visualized in the form of various diseases in human and loss of biodiversity. The population burst is the root cause of imbalanced ecological system because agricultural land decreases drastically. Therefore we must aim to develop and improve the agri- economic growth of farmers by introducing elite variety of Mucuna pruriens (L.) DC. M. pruriens, a member of Fabaceae family, is an adaptogen and known for its tonifying, strengthening and all around beneficial properties from every part of the plant. M. pruriens has range of medicinal applications, because of unusual amino-acid L-DOPA (L3, 4dihydroxyphenylalanine) a precursor of neurotransmitter dopamine (Anti Parkinson’s) and involved in various secondary metabolite pathways. It acts like aphrodisiac, anti-snake venom, anti-depressant, antidiabetic, anti-microbial. It has a nutritional quality comparable to soyabeans. M. pruriens exhibits a tolerance for various kinds of environmental stresses such as drought, low soil fertility, and high soil acidity. M. pruriens suppresses weeds through indeterminate growth and large leaves reduce the amount of light for weeds. It shows allelopathy and effective in lowering nematodes. M. pruriens is used as forage, fallow, green manure prevents the soil erosion. It has a longer time span to be used in rotations for management of various pathogens.

ABSTRACT

Commercially valuable plants are one of the main sources of economy. Growth, development and yield of these plants are severely affected by various environmental factors, which depend on dynamic fluctuations in different parameters. To counteract these responses, plants try to adapt evolutionarily but one form of adaptation is never going to suffice the simultaneous attack of multiple stresses. Crossing and plant breeding approaches came to practice, which is quite beneficial but requires lot of screening and is manpower consuming. In recent era new approaches have been developed combining recombinant DNA technology with tissue culture techniques to obtain improved varieties with specific desired traits. This method had an edge over other technologies but it still carries lot of complications. It is evident that genes do not act alone but act cooperatively and remain in continuous influence of other factors. So to target a particular gene for improved variety development requires a lot of detailed micro-details. In this communication, an attempt have been made to discuss mainly how transcription factors (TFs) have been targeted for this purpose. TFs in many occasions act as master regulators or central players of a particular pathway. This is attained by their ability to interact with multiple cis-elements present in the promoters of downstream stress or metabolism responsive genes. This behavior sometime leads to occurrence of some unwanted traits, which should be kept in mind to develop transgenics. Here, we focused mainly on important TF families such as ERF, WRKY, NAC and bHLH. We have been an attempt to analyzed how these TFs influence growth, yield and stress response of commercially valuable plants such as rice, wheat, tomato and cotton. This section will help to understand the genetic manipulation of plants can be targeted for improved variety development.

ABSTRACT

The people meet their need by taking pulses as their daily meal and therefore, the pulse play a significant role as food supplements. Pulses are called ‘Nutrient Powerhouse’ and are edible food of plants in the legume family. It contains high protein, fibers, various vitamins and amino acids. Starch is the main form in which plants store carbon. It occurs as semi crystalline granules composed of two polymers of glucose called amylose and amylopectin, depending on the plant organ. Starch granules are characterized by internal growth rings. Starch grain is a very useful taxonomic tool in plant systematic. In this investigation we atudied the morphological variations of starch grains in seeds. These starch grain contain the composition of carbon to form different structural diversity of seeds like simple i.e. two types: concentric and eccentric, semi-compound and compound. Polarized microscopy (PM) was used for better micro-morphological (Maltase cross) study. These micro-characters are carried out by UPGMA and construction dendrogram for visual appreciations of taxonomic relationship.

ABSTRACT

Soil salinity is one of the most serious environmental issues that limit agricultural productivity. Most of the food crops belong to glycophytes and hence, they are not able to withstand salinity stress. Salt stress significantly affects the various morphological, biochemical and physiological processes of the plant which leads to poor plant growth. Therefore, there is an emergent need to find out mitigation strategies to cope with the deleterious impacts of salt stress. Plant breeding techniques, efficient resource management and genetic engineering have been employed to cope with salt stress but these were of limited success as salt tolerant trait is complex both genetically and physiologically. Therefore, using plant growth promoting bacteria seems to be a cost effective and sustainable approach for salinity stress management. Hence plant growth promoting bacteria could play a significant role in alleviating salt stress. This chapter endeavor to provide an overview of the various important mechanisms such as facilitation of nutrient uptake, production of phytohormones, ACC deaminase production and siderophore production employed by the plant growth promoting bacteria to enhance the plant growth under salinity stress.

ABSTRACT

The deeply rooted coastal grass Spinifex littoreus (Burm.f.) Merr. is a potent soil binder having capability to form a strong and stable dunes. However detailed features of this species has not been thoroughly studied till date. In this communication, salient morphological features along with different crucial micro-morphological peculiarities have been discussed. The comparative analysis of gradual degradation of population of Spinifex littoreus and native dune vegetation of the coastal belt in East Medinipur of West Bengal and adjacent Balasore district of Odisha are provided, along with different biodiversity indices. Plants were characterized as stout, dioecious, stoloniferous grass upto 90 cm tall, with rigid and sharp spiny leaves. Spikelets in racemes subtended by large bract-like spatheoles which are fascicled into large capitate spiny structures 30-37 cm in diameter. S. littoreus is fairly common along sandy shores and dunes. Plants are useful as a sand binder in unstable coastal dunes ecosystem.

ABSTRACT

Artemisia annua L. is known for the content of anti-malarial drug, artemisinin. It is widely distributed in subtropical, temperate and subtropical zones worldwide and traditionally used for the treatment of various ailments. Artemisinin has been the frontline treatment since the late 1990s and saved countless lives, especially among the world’s poorest children. The major chemical constituents of Artemisia annua L. are sesquiterpenoids and some phenolics. The essential oil of Artemisia contains artemisia ketone, camphor, caryophyllene oxide, 1,8- cineole and -pinene, and these essential oils constituents are known for their medicinal properties.Artemisinin was discovered in 1971 bya Chinese Medicinal Chemist Youyou Tu (Nobel Prize in 2015). As the demand for artemisinin remains high worldwide, exploring the chemical and the genetic variation especially knowledge, about its mechanisms for high-yielding would be more important. The plant extract is also known for other biological activities such as anti- hypertensive, anti-microbial, immuniosuppresive, and antiparasitic activities. To increase the production of artemisinin and to investigate the effect of climate change on artemisinin content will be the major research area in future from Artemisia genus. Exploring the alternative resources such as microbial production and semisynthetic approaches from other easily available natural molecules would be of high demand. Some other molecules from Artemisia should also be focused for the future development of man kind and in drug discovery science. An overview of present status of medicinal applications, phytochemistry, and future perspectives for the research possibilities on the active ingredient Artemisinin from A. annua is discussed in this chapter.

ABSTRACT

Due to the exorbitant price of the popular gelling agent agar, Plant Tissue Culture (PTC) has gradually become a costly technique in recent times. So, alternative sources of agar needs to be popularized which at one hand should be able to meet all the positive properties of agar and on the other hand be cost effective as well. The seed husk obtained from Plantago ovata Forsk. is such an alternative which was successfully used as a gelling agent for media preparation in PTC experiments for in-vitro shoot and root development in Nicotiana tabacum L. and Ocimum gratissimum L. The nodal explants were grown in MS basal medium (MSo) supplemented with 3% sucrose and were gelled with progressive concentrations of isabgol husk to ascertain the optimum concentration for better survival and growth response, while 0.8% agar acted as a negative control. The response of the explants on media gelled with husk was compared to that of media gelled with 0.8% agar. It was inferred that 0.3% husk in the medium was optimum for best growth response which was comparable to that of agar. Consequently, the estimated cost of a liter of MSo reduced by almost Rs.61.15/- (compared to Himedia’s Agar) to Rs.177.57/- (compared to Sigma’s Agar) after using isabgol seed husk as a gelling agent in media preparation.

ABSTRACT

Agrotechnology of banana has been introduced for the first time by CSIR- Indian Institute of Integrative Medicine Jammu for commercial cultivation of banana in Himalayan Shivalik range. The aim of the present investigation is to study the potential of Tissue Culture (TC) banana production in a diverse environment of Shivalik range, to make the state self-sufficient in banana production, employment generation and generate revenue for the farmers and to studies on the post harvest handling and marketing of TC banana. Initially, the sapling of this high quality tissue culture variety {BHIM Grand naine (G-9)} Banana was brought from Agro-division of Cadilla Pharmaceuticals Limited Ahmedabad, Gujarat. The field experimental trial was conducted during 20016- 2017 at CSIR-IIIM Research Station, Chatha Farm, Jammu in which four treatment combination arranged in Randomized Complete Block Design (RCBD). Significant differences were observed among the treatments for all the parameters studied. Among the date of planting, banana planted at 10th August reported significantly highest pseudostem height, pseudostem girth, more average number of leaves/plant, first flower emergence (days), first finger ripening (days), more number of finger per hand, more number of hand/ branch, higher weight of finger/hand (kg), higher weight of single finger (g), more finger diameter (cm), higher length of finger (cm), more weight of finger/ bunch (kg), more percentage of plant harvested at a time, higher yield (64.22 tonnes/ha), gross return (Rs. 12,84,400/ha), net return (Rs. 9,58,730.5/ha) and B:C ratio (3.94) as compared to other treatments. On an average the yield of per plant was 20-30 kg and 50- 64 tonnes per hectare. In terms of economy as per market analysis, price of banana in Jammu is approximately Rs. 20 per kg. Thus, on an average it gave Rs. 250-300 per banana plant. On the basis of market demand, approximately Rs 6.17- 9.50 lakh net return can be obtained by cultivation of one hectare of land which is alternative business for the farmers of the Jammu and Kashmir. This may leads to revolution of banana cultivation in Shivalik range of Himalaya.

ABSTRACT

In the present investigation on three different plant species from pollution and control area were carried act for biochemical analysis such as total chlorophyll content, leaf extract pH, relative water content and ascorbic acid test. The results indicate accordance to order of tolerance index of plant species in air pollution are Macrosolen cochinchinensis, Loranthus parasiticus and Viscum album. The changes in pollution tolerance index are biochemically induced due to the surrounding environmental conditions. Thus, it is observed that Macrosolen cochinchinensis is highly tolerant species among the selected three species. Thus, in future this plant species may play an important role in pollution indicator. APTI analysis show the pollution tolerating decreasing order for there species can be Macrosolen cochinchinensis> Loranthus parasiticus>Viscum album and as ascorbic acid result indicate that Macrosolen cochinchinensis show mostly defence mechanism and highly tolerance level against different kind of pollutant. Besides these three hemiparasite plant species have some valuable ethnomedicinal important. In India tribal people used leaf and stem bark in curing abortion, miscarriage, vaginal bleeding during pregnancy, treat circulatory and respiratory system problems.

ABSTRACT

Dysoxylum binectariferum (Roxb.) Hook. is an evergreen tree, indigenous to India and widely distributed in different parts of India. Primarily, this plant is used in the timber market for the preparation of furniture and other wooden goods. Apart from utility in timber market, it has tremendous medicinal properties because of its unique secondary metabolites. Its hydroalcoholic extracts possess a wide range of pharmacological activities including immunomodulatory, anti-inflammatory, anti-cancer, anti-leishmanial, etc. The chromone alkaloid rohitukine, the major constituent of the plant, possess anti-fertility, anti-leishmanial, anti-adipogenic, anti-ulcerogenic, anti-cancer and immunomodulatory activities. The most extensively studied and advanced therapeutic property of this chromone alkaloid is its cyclin-dependent kinase inhibition activity, which has a direct link with its anti-cancer effect. Inspired by this natural product, two clinical candidates (flavopiridol and riviciclib) are discovered and studied in human cancer patients for treating various types of cancer. Another preclinical lead IIIM-290, which is directly derived from rohitukine via synthetic modification, has demonstrated promising oral efficacy in animal models of various cancer types. The high-value medicinal applications of this plant have necessitated the need of bringing this tree under captive cultivation. The present chapter discusses the biology pharmacological and secondary metabolites present in Dysoxylum binectariferum. The preclinical and clinical leads derived or inspired from rohitukine are also discussed in this communication.

ABSTRACT

Bunium persicum (Boiss.) Fedtsch., (Family: Apiaceae) is an important plant of temperate regions and its seeds are used as carminative substance in various food recipes. The seeds of the species act as a good source of economy to the hilly and mountain people. As the species is restricted in its habitat, therefore, it requires mass cultivation practices. Many attempts are already made to cultivate species near to its natural habitat. In recent years some efforts have been done to domesticate this plant in certain areas of India like Shong area in Kinnaur district of Himachal Pradesh, India, but the cultivation remained very limited. Therefore, in addition to its collection from the wild sources the species needs more scientific intervention in agriculture sector. This species has typical Palaeo-Mediterranean distribution. In India the species is distributed in wild only in North West Himalaya (J&K, HP and UK) which makes its ocurrence rarity in the country. The species restricted its distribution between 1850-3100 meters above mean sea level. Phylogenetically the species is near to Carum carvi L., therefore, C. carvi is the potential adulterant of B. persicum.

ABSTRACT

Magnolia campbelii Hook.f. & Thomson is a deciduous tree species belonging to Magnoliaceae and is highly valued for its ornamental uses. The species is threatened by habitat degradation as well as its utility as timber. It is distributed in the eastern Himalaya, southern Myanmar, and Yunnan province of China. However, its exact distribution in the eastern Himalaya is unknown owing to rough terrain and remote location of the Himalayas. We modelled the environmental niche and delineated the potential habitats and distribution areas of the species in this study using ENVIREM dataset and Maxent software. Thermicity Index and Topographic Wetness were the critical environmental variables determining the species distribution in the eastern Himalaya as revealed by Jackknife analysis. Geographical projection of the modelled environmental niche indicates that most suitable areas for its distribution are the western part of Arunachal Pradesh, few pockets in Sikkim, and the central part of Bhutan. The results of the study have implications for in situ conservation of the species as well as identifying suitable areas for reintroduction.

ABSTRACT

Tinospora cordifolia (Willd.) Miers ex Hook.f. & Thoms. is a versatile liana rich in various active compounds having diverse chemical structures. Limited research has been done on the biological properties and the probable medicinal applications of the individual isolated molecules actually responsible for the medicinal nature of the plant. Recent advances in the discovery of active constituents from T. cordifolia and their biological impact in disease cure has led to an increase interest on this plant across the globe. The therapeutic virtue of giloy used in traditional and Indian System of Medicine (ISM) has been well established through advance testing and evaluation, both pre-clinical and clinical trials in different diseased conditions. These studies make this indigenous drug plant a novel candidate for bio-prospection and drug development for the treatment of diseases such as cancer, ulcers, liver disorders, heart diseases, diabetes and post-menopausal syndrome where sufficient treatments are still not available. Over the past few decades, a noteworthy progress has been achieved in cancer prevention and treatment, and still there remains a need for effective treatment program. One of the best possible approaches with immense potential is chemoprevention, which involves the use of natural, synthetic or biological molecules to suppress, reverse or prevent cancer or the progression of cancerous cells. It is most likely that many agents present in specific herbs, herbal products and natural dietary factors will have an effect throughout the process of carcinogenesis. Thus, a detailed chemical investigation with the aim of isolating novel molecules, their structure elucidation, quantification, validation and pharmacological evaluation in terms of its anticancer potential was carried out on T. cordifolia. The present study was designed to isolate and identify new promising anti-cancer candidates from the aqueous alcoholic extract of T. cordifolia using bioassay-guided fractionation.

ABSTRACT

Traditional herbal drug preparations are known for centuries to protect several human diseases such as peptic ulcer, diarrhea, piles and dysentery. Current days knowledge about the underlying biochemical mechanism for most of the gastric ulcers and majority of the duodenal ulcers deserve appropriate consideration and due weightage while consolidating regarding the efficacy of a plant extract. The present investgation relates to a pharmaceutical composition comprising an effective amount of lyophilized extract or at least one bioactive fraction obtained from flowers of Woodfordia fruticosa (L.) Kurz, along with one or more pharmaceutically acceptable additives or carriers. The present results provides a composition for treating ulcers such as stress induced ulcer, peptic ulcer, cold restraint induced ulcer, drug induced ulcer and acid induced ulcer, and also the composition used as specific inhibitor of gastric H+, K+-ATPase. Herbal formulation in the form of capsules were conducted on marker-based stability studies as per ICH Q1 guidelines at accelerated and real time stability studies (40±2°C, 75% ± 5% RH and 30±2°C, 65%±5% RH, respectively), and also stability testing investigation was done as per stability specifications at intervals of 0, 3, 6 months for accelerated stability studies and 0, 3, 6, 9, 12, 18, 24, 36 months for real time stability. The present data reveals that this species is one of the potent Indian medicinal Plant which helps in curing ulcer and associated diseases.

ABSTRACT

Mushrooms are in use for centuries in traditional system of medicines, and have importance in greater Chinese and western pharmacopeia. Morchella species are well known across the globe as a popular and prized edible fungi and unique culinary saprophytes. They have a long history of usage in the formulation of different traditional medicine for centuries due to their anti- oxidant, anti-inflammatory, anti-cancerous and immuno-stimulatory properties. Inspite of high demand and day-by-day increasing importance, cultivation of M. esculenta Dill.ex Pers and other morels are limited, however, there is a report that some species of Morchella are successfully cultivated in China. Phytochemical studies reveals that polysaccharide groups, phenolic compounds, tocopherols, ascorbic acid and vitamin D are the most active molecules present in morels. In addition to these compounds, morels are also rich in carbohydrates, amino acids, fatty acids, organic acids and several kinds of macro-and micronutrients. Literature revealed that no data on reproductive biology of M. esculenta are available till date. Future investigation is required to study the life cycle and the ecological association of different morels to high altitude regions, and causes of their association with coniferous and broad leaved trees such as Cedrus deodara, Pinus gerardiana, Picea smithiana, Quercus and Castanopsis species. There is an urgent need to explore all traditional knowledge associated with morels, phytochemistrys and associated pharmological studies of major compounds, reproductive cycle and their basic biology for future conservation of this precious mushrooms for man-kind.

5.1 Introduction

Quality is one of the major positioning tools of the commodity for marketability, profitability and for consumer satisfaction. The relative importance of each quality component depends upon the commodity and its intended use and varies among growers, handlers and consumers. To growers a given commodity must have high yield and good appearance and must withstand long distance transportation to market whereas, appearance of quality and longevity is important from the point of view of wholesale and retail marketers. Terms commonly used in measurement of keeping quality of cut flowers are longevity, shelf-life, vase-life, and lasting quality. In addition to these, several criteria have been used for evaluation of post harvest flower quality like:

4.1 Overview of Concept and Science

Preserving flowers and plant parts has been a form of artistic expression that was very popular in Victorian ages. Now, this process is moving from an art to a highly interdisciplinary science and has become a major economic activity on a global scale. Because, dry decorative products have been globally accepted as natural, eco-friendly, long lasting and inexpensive as compare to fresh flowers and plant parts, which are perishable and delicate in nature. These perishables cannot retain their fresh like appearance and beauty for several months and it becomes prodigious job to maintain these qualities. Even with preservatives, the vase life of fresh cut flowers has been prolonged to an extent of 40 per cent only. Hence, for those who want the beauty of nature around them in the form of flowers but on a more permanent basis dried flowers are a solution.

Domain of Dried Flowers

Anything from botanicals such as stems, twigs, branches, bark, leaves/ foliage, flowers, thorns/ spines, fruits, cones, seeds, roots; and minor forest products like lichens, fleshy fungi, mosses, selleginnela ferns etc. in a dried form come under the domain of dry flowers. Until a few years ago dried flowers were popular in the West with very few homes in India opting for them. But, today Indian homes have taken to dried flowers in their many forms and shapes like duck to water. There are different segments which are covered under dried flowers (Table 1).

3.1 Introduction

Flower and plant part based perfumes are receiving special attention at this modern age of civilization. With pre-eminent senses of smell or taste, a large brain affording a superior intellect, would lead to the use of these abilities to improve the environment, the flavour of food and the smell of the surroundings. In fragrance industry, essential oil is a generic term given to all fragrant extracts such as essential oils, absolutes, resinoids, concretes etc. Since antiquity, flowers and plant parts have been used as flavours for food, for religious rites, for beautification of homes and gardens, as incence and insect repellent, in cosmetics and personal adornment and in medicine. Fragrance compositions are used with the purpose to affect customers through their sense of smell and the job of composing fragrances is left upto an expert known as “Perfumer”.

Despite continuing invention of synthetic fragrant extracts, natural essential oils still remain the most important part of fine fragrance. Essential oils are so aromatic and are the focus of the fragrance industry. The content of a pure essential oil product gives it an unmistakable aromatic and healing quality. Nature has always provided us with food, medicine and fibre for clothing that we need to survive. Sometime a walk in the woods, a day spent swimming in Local River or lake or a beautiful sunset can lift a person’s spirit and give them a new outlook. The aroma of a pure essential oil can do the same.

Flavour and fragrance have now become every day necessities and their demand is constantly increasing across the world. Products from different extraction methods are known under different names even though their basic materials are the same. For instance, orange blossom from citrus aurantium on solvent extraction produces “orange blossom absolute” but that of steam distilled produces “Neroli oil”.

2.1 Introduction

Perfumery, the art of making perfumes, began in ancient Egypt but was developed and further refined by the Romans and the Arabs. The history of perfume began in antiquity. The word perfume is used today to describe scented mixtures and is derived from the Latin word, “per fumum”, meaning through smoke. Although perfume and perfumery also existed in East Asia, much of its fragrances are incense based. The world’s first recorded perfume maker Tapputi was mentioned in a Cuneiform tablet from the 2nd millennium BC in Mesopotamia. Perfume and perfumery also existed in India. The earliest distillation of Attar was mentioned in the Hindu Ayurvedic Charaka Samhita. The Bible describes a sacred perfume, consisting of liquid myrrh, fragrant cinnamon and cassia. In Islamic culture, perfume usage has been documented as far back as the 6^th century and its usage is considered a religious duty. Al-Kindi in the 9^th century wrote a book on perfumes entitled ‘Book of the Chemistry of Perfume and Distillations’ that has more than hundred recipes for fragrant oils, salves, aromatic waters and substitutes or imitations of costly drugs.

1.1 Importance

Perfumes, essential oils and aroma are some of the products which indicate religious values, living standards, personality development for personal use and adornment from years back. The Vedic literatures also indicate the presence of perfumes and aromatics and were used for religious purposes as well as medicinal purposes. The word perfume has been used today to describe scented mixtures and is derived from the Latin word, “per fumum”, meaning through smoke. Perfumery has been one of the Indian arts and was a famous centre for perfume and perfume products. The essential oil means a class of volatile odoriferous oils, obtained from raw material of botanical source that impart the plant odour and other characteristic properties derived through distillation (usually with water or steam) or expression and obtained in the form of an oily liquid. The oils extracted through expression are some time called “Expression oils”. The aromatic substances being used from the Vedic period are defined as those plants which possess odoriferous steam volatile constituent’s (essential oil) in them.

Brazil, China, Egypt, India, Indonesia and USA are the major producers of aromatic materials. China is becoming increasingly important as a primary source and a very large quantity of aromatics and flowers as raw material is available. European Union and Switzerland are the main world markets for aromatics and also has established as a largest consumer of essential oils. The world’s largest importer of aromatic materials is the USA followed by Japan. The latter accounts for about 10% of world demand especially for lemon, clove, orange, peppermint and cedar wood oil.

India exports essential oils of lemongrass, mint, sandalwood, palmarosa, cardamom, ginger and eucalyptus in adequate quantity under AGMARK certification. But, for a better export quality there is a need to study the customer requirements, quality assurance and standards to keep pace with International market, Total Quality Management (TQM) through adulteration detection and modern analysis techniques with economic marketing channels.

India possesses a number of resources as plant parts, ample land and varied climatic conditions with low production costs but the essential oil producers need to study the customer needs for a regular supply of best quality products at a competitive rates. Today, second important factor for successful competition is the quality assurance. The consumers, these days, are very much aware about the quality and safety of using perfumes, essential oils and other pharmaceutical products. The third most important point is the packaging which attracts the consumers most of the times. Proper packaging along with an attractive label is always a plus point for the marketing of any product. Hazard Analysis Critical Control Point (HACCP) and Good Manfacturing Practices (GMP’s) are the quality assurance systems which continuously controls and regulate the quality production of commodities, needs strict enforcement. ISO 9000, TQM and ISO 14000 series are the some of quality management organizations, working for the development of quality standards.