eChapters

Q1. Resistance in tetracycline is due to mutational changes in

1. Tet A gene
2. Kat G
3. Par C par E
4. Inh A

Q2. Single step mutation can be seen in

1. Erythromycin
2. Tetracycline
3. Chloramphenicol
4. Rifampicin

Q3. Multistep mutation is observed in

1. Streptomycin
2. Rifampicin
3. Both A and C
4. Tetracycline

1.1 Introduction

Broadcasting seed and harvesting meagre yields was a common method of ancient agriculture. High use of developed crop varieties, irrigation, chemical fertilizers, pesticides and farm mechanization is the main thrust of modern agriculture. Therefore, modern agriculture is an energy intensive farming system (Khan et al., 2010). Though modern technology based agriculture is achieving success to meet food demand of increasing global population, yet it creates many environmental issues such as loss of soil fertility, salinization and water scarcity.

Protection from natural enemies and adverse environmental and climatic conditions is vital to safeguard insects and to this effect, insects possess a great variety of protective structures and mechanisms without which they cannot survive.

    i)    Food. Each insect species has certain nutritional requirements for the completion of life cycle. If the required food for any particular species gets reduced in a particular locality, that species will gradually disappear from that locality, though other environmental conditions may be favorable. Under normal conditions, there may be sufficient food for the insect to live on and continue its race. In the event of sudden increase in the population of an insect, the density of the population becomes too high to be supported by the food available in the area. Under such conditions, some individuals of the species either migrate to other suitable areas where enough food is available or else starve, if the species has limited power  of  dispersal  as  in  the  case  of  many  coccids.  According  to  the nutritional requirements insects are characterized as ‘omnivorous’ (feeding on different kinds of both plant and animal food), ‘carnivorous’ (feeding on animal food) and ‘herbivorous’ or ‘phytophagous’ (feeding on living plants). Those insects, which feed on many cultivated and wild species of plants, are termed ‘polyphagous’ (e-g) Locusts, grasshoppers, cutworms etc. If the feeding activities of a particular species of insect are confined only to plants of one botanical family, it is termed ‘oligophagous’ (e-g) cabbage butterfly - Pieris brassicae. When only a single species of plants constitute the food of a particular species of insect, it is termed ‘monophagous’ (e-g) Brinjal fruit borer.

Agroforestry systems are not simply systems where trees and crops or animals give useful products to the farmers, rather systems where trees and crops and/ or animals interact. Interaction literally means influence or mutual or reciprocal action. So component interaction refers to the influence of one component of a system on the performance of other component as well as the system as a whole. In agroforestry systems, trees are grown in close proximity to crops and pasture. Their performance would largely depend on their ability to share various growth resources in a given environmental situation. Various interactions take place between the woody trees and herbaceous plants (crops or pastures) which is referred to as tree-crop interface. These interactions take place through the media of soil and microclimate and may exert favourable or adverse effects on the crop. Study of interaction helps to know how the components of agroforestry utilize and share the resources of the environment, and how the growth and development of any of the components will influence the others. Interaction occurs both above and below the ground and includes a complex set of interactions relating to radiation exchange, the water balance, nutrient budget and cycling, shelter and other microclimatic modifications.

Concept: Operations conducted before and after harvest are essential components of the system for producing high-quality seeds. If enough care is not taken in the initial stage, there most likely won’t be any resources left to get high-quality seed. Like this, carefully produced, high-quality seed may lose a significant percentage of its worth if post-harvest management protocols are not adhered to. Its quality is highly correlated with soundness, fullness, and homogeneity of physical characteristics (size, shape, density, colour, and appendages), in addition to mechanical planting’s simplicity and improved crop establishment. Improving the seed that has been gathered and getting it ready for secure storage till needed are accomplished through the effective and efficient process of seed processing. To increase the quality of the seed lot, the goal of seed processing must be to optimize the physical properties of the seed and the constituents of the harvested seed mass. The three main steps in seed processing are conditioning, grading, and applying protectants or enriching with nutrients. These steps are completed in compliance with established protocols and standards (McDonald and Copeland 1997; Agrawal 1996; Jorgensen and Stevens, 2004). The distinct qualities of the crops and the surroundings are taken into consideration while adjusting the machines and operations.

The skin of the birds provides a physical barrier between the birds its environment, protecting the birds from bacteria and physical injury. The health of the bird can be compromised, and the value of the end-product can dramatically decrease. Bird’s skin differs from that of mammals by its thinness, by the presence of feathers instead of hair, and by the absence of sebaceous glands (sweat glands), although the overall histological structure is similar. Bird’s skin is composed of an epidermis separated from a dermis by a basal membranes to protect the body from infection. The integumentary system consists of the skin, the feathers and the appendages (claws and beak). The skin covers the majority of the body and contains glands in the outer ear canal and the preen gland at the base of the tail, that the bird uses to preen its feathers. The integumentary system is very important in providing protection to the bird from a number of potentially dangerous situations. The functions provided by the integumentary system include: A barrier between the external environment and the internal systems and organs thus provides support and protection from infection by microorganisms and from physical injury. Excellent thermal insulation to help regulate body temperature in a variable environment. Numerous nerve endings for the senses to enable the bird to be aware of potentially harmful situations. Pigments for display and protection from the elements. The compounds capable of conversion into vitamin D when exposed to sunlight. Avian skin is relatively thin with the epidermis consisting of only 2-10 cell layers. There are no sweat glands; instead heat is lost via the respiratory tract and by radiation from featherless areas. The brood patch is a featherless area that can occur in both sexes and is either seasonal or permanent depending on species.

Natural Farming (NF) represents a sustainable approach to agriculture that draws upon ecological principles, minimizes reliance on external inputs, and makes extensive use of locally available resources. In contrast to conventional farming—which often depends on synthetic fertilizers and commercial animal feed—natural farming seeks to foster balance among the soil, crops, animals, and microbial life. Popularized in India by Subhash Palekar, this method promotes the use of natural preparations such as Jeevamrit, Beejamrit, and practices like mulching to enrich soil fertility and support plant vitality.
A core aspect of natural farming is the inclusion of livestock, which significantly contributes to nutrient recycling, weed suppression, and overall farm efficiency. Products like cow dung and urine form the basis of microbial formulations that boost soil activity and crop productivity. Livestock also contribute to ecological health through regulated grazing, pest management, and the breakdown of organic residues.
Animals such as cows, buffaloes, goats, sheep, backyard poultry, and fish are effectively integrated into the NF system to form a cohesive and regenerative agro-ecosystem. These animals enhance crop systems by providing organic inputs while simultaneously benefiting from pesticide-free fodder and farm-generated feed, leading to healthier livestock and improved quality of dairy, eggs, and meat.
This interdependent model reduces the need for external feed inputs, enhances natural animal wellness, and raises farm income, particularly for smallholders. Natural farming promotes the use of native breeds that thrive in local conditions with minimal intervention.
Ultimately, integrating livestock within natural farming is not just beneficial— it is vital for establishing robust, regenerative farm systems. This synergy between animals and crops supports ecological sustainability, strengthens food systems, and uplifts rural communities.

Abstract

Plant genetic resources constitute the chief component of agro-biodiversity and comprise of land races, modern cultivars and obsolete varieties, breeding lines, genetic stocks and wild species. They provide the basic materials to the plant breeders to utilize genetic variability for the development of high yielding cultivars with a broad genetic base. The utilization of these genetic resources, however, depends upon their efficient and adequate characterization and evaluation, which in turn entails efficient characterization standards and appropriate strategies.

Introduction

Analysis of trait data generated from characterization and evaluation of the genetic resources is used to understand and use diversity. Currently, a large number of distance measures are available for analyzing similarity/dissimilarity among accessions based on different traits representing different types of variables. The selection of the most appropriate distance measure for each trait is the prerequisite for diversity analysis. One of the approaches is to form clusters where accessions between clusters would be more diverse than the accessions within a cluster. The clustering algorithms require a distance/similarity matrix between the accessions which can be calculated depending upon the nature or type of traits such as morphological and agronomic traits and/or molecular markers.

The diversity of the haloarchaea found within a crystallizer pond of a solar saltern located in Siridao and Ribandar, Goa, India was examined. Both cultured-based and culture independent methodology were employed which included  surface spreading of samples on different media containing 25% crude salt/NaCl, DNA extraction from sediment and water samples, amplification of 16S rRNA gene and DGGE.

Anar butterfly, Deudorix isocrates (Fab.)

The pest has been found boring into fruits of pomegranate. Besides it may attack guava, aonla, apple, ber, citrus, litchi, loquat, sapota, etc., pomegranate is the most preferred host for this pest. It may destroy up to 50 percent fruits. The female lays eggs singly on calyx of flowers or small fruits. On hatching, the caterpillars bore inside the developing fruits and are usually found feeding on pulp and seeds just below the rind; subsequently bacteria and fungi causing fruit rot also attack the infested fruits. The conspicuous symptoms of damage are offensive smell and excreta of caterpillars coming out of the entry holes, the excreta is found stuck around the holes. The affected fruits ultimately fall down and are of no use; even if the fruits be picked before falling down, these fruits have no market value.

2.1. What is Greenhouse Technology?

Greenhouse technology involves primarily the structural design that can take care of climate control mechanisms to create proper micro-crop-climate and secondarily it involves the technology needed for optimum growth and production of crop grown under this structure. Combination of these two aspects may be expressed as ‘greenhouse technology’.

So the ‘greenhouse technology’ may be defined as “The technology required to built a structure, covered fully, partly or porously for manipulating the inside climatic conditions, under which crops/plants can be grown with special measures”.

Veterinary physiology deals with the study of normal functions of animal systems. It includes the study of how animals work and the physical and chemical processes that occur within animals. The examples of various biological processes in animals include gas exchange, blood and circulation, osmoregulation, digestion, nervous and muscle systems, respiration, growth, reproduction, endocrine function and how animals behave and function in different environmental conditions. This also includes the possible manipulation of the reproductive processes by means of hormone manipulation techniques for more efficient livestock and poultry production.

DNA Sequencing

DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA. Sequencing can be done on a short fragment of DNA to cover the entire genome. The maps thus constructed using the nucleotide sequences in the correct order is called as sequence map.

In 1977, twenty-four years after the discovery of the structure of DNA, two separate methods for sequencing DNA were developed: the chain termination method (Sanger et al., 1977) and the chemical degradation method (Maxam and Gilbert, 1977). Both methods were equally popular to begin with, but, for many reasons, the chain termination method is the method more commonly used today. In chemical degradation method, chemicals specific to each base remove the last base, which has been labelled. The method uses toxic chemicals which required high care while handling.

In chain termination method, DNA polymerase extends a DNA strand in which dideoxynucleotide becomes incorporated terminating the chain extension (phosphodiester formation) at the 3’OH site making an array of fragments differing in length. This method is based on the principle that single-stranded DNA molecules that differ in length by just a single nucleotide can be separated from one another using polyacrylamide gel electrophoresis.

3.1. Cereals and millets

3.1.1. Rice

1. Major weeds

Grasses

Concept: The preservation of viable seeds from the moment of collecting until they are needed for sowing is known as storage. Every year, a significant quantity of seeds loses their usefulness due to incorrect storage. Seeds need to be preserved and stored dry. As was previously mentioned, seed moisture content and storage temperature have the biggest effects on how long seeds last. The three main environmental elements that impact seed deterioration and loss of viability are moisture, temperature, and oxygen percentage. For over 90% of species, reducing seed moisture content (MC) to certain thresholds consistently lengthens the seed’s lifespan (Roberts 1973). These species are categorized as “orthodox” in terms of the conditions in which they should store seeds; under suitable dry, cool conditions, they typically maintain viability and germinability even after extended periods of storage. Ellis and Roberts’ improved viability equations have addressed the quantitative impacts of both drying and cooling for orthodox seeds. The life of the seed is often doubled for every 1% drop in seed moisture (when seed MC fluctuates between 5 and 14%) and every 5 oC drop in storage temperature (between 0 oC and 50 oC) (Harrington 1972). Low seed MC and low temperature are hence fundamentals of traditional seed storage. Careful regulation of these two factors is necessary to achieve optimal storage life. But most seed produced annually just has to be kept from harvest until the following planting season. Depending on the type of seed, it may or may not need to be stored at temperatures and relative humidity above room temperature. Regardless of any modifications made for unique uses, the local climate storage facility needs to have a few essential components (Barre, 1954; Wheeler and Hill, 1957).

Introduction 
Toxicological studies play a crucial role in assessing the safety and potential hazards of chemicals, pharmaceuticals, and environmental pollutants. To ensure the reliability, consistency, and integrity of such studies, the concept of GLP has been established. GLP is a set of principles that govern the planning, execution, monitoring, recording, and reporting of laboratory experiments, particularly those related to safety assessments. The primary objective of GLP in toxicological studies is to ensure that the data generated is of high quality, reproducible, and acceptable to regulatory agencies worldwide. This is essential for evaluating the potential risks associated with exposure to various substances, aiding in the development of safer drugs, chemicals, and consumer products. Compliance with GLP guidelines minimizes errors, prevents fraudulent practices, and enhances transparency in research, ultimately contributing to public health and environmental safety. The assessment of potential hazards posed by chemical, pharmaceutical, agrochemical, and other substances to human health and the environment lies at the heart of toxicological studies. These investigations, conducted in non-clinical settings, provide crucial data that informs regulatory decisions, guides product development, and ultimately safeguards public well-being. The consequences of inaccurate or unreliable toxicological data can be far-reaching, potentially leading to the approval of unsafe products or the unwarranted restriction of beneficial ones. Recognizing the critical importance of the integrity of these studies, the concept of Good Laboratory Practices (GLP) has emerged as a cornerstone, establishing a robust framework for their planning, conduct, monitoring, recording, reporting, and archiving

The potato (Solanum tuberosum L.) is one of the four most important food crops of the world, the other three being rice, wheat and maize. It is a high yielding and nutritive crop and produces highest dry matter, carbohydrates, edible protein, minerals and vitamin C and B per unit of area and time. In view of the shrinking cultivable land area and increasing population of India, the potato has a high potential to solve the problems of hunger and malnutrition in our country. But, potato is a perishable commodity and its harvest time (February/March) in Indo-Gangetic plains (which account for nearly 87% of the country’s potato production) coincides with steep rise in ambient temperatures. From April onwards, temperatures start shooting up and the produce has either to be consumed within a short period of time or is required to be preserved in the cold stores. Due to inadequate, expensive and unevenly distributed refrigerated storage facilities in the country, there have been frequent gluts in the market causing substantial economic losses to farmers and wastage of precious food. Such gluts occur every 3 to 5 years. The glut years are followed by a reduction in potato area and production leading to high potato prices in alternate years which again encourage farmers to increase potato area in subsequent years resulting again in a glut situation and price crashes. This boom and bust cycle has thus continued for long (Pandey et al., 2009).

Introduction

Low and erratic precipitation, diurnal fluctuations in temperature and low humidity are the typical characteristics of deserts and their margins. Further, strong winds, sunny and very bright days and unpredictable periods of drought and famines are regular features. These lands are poor in fertility. Uninterrupted increase in human and animal pressures is violating carrying capacity of the natural resources, thus resultant is desertification.

Arid zones are described as a part of the drylands, and have the most severe edapho-climatic conditions. The major distinguishing feature for defining and for planning for the arid zone is the low rainfall (below 500 mm or Aridity Index <0.20) with more than 50% inter-annual variability. This makes a great difference in terms of the nature of the ecosystem, the socio-economic environment and the challenges for sustainability. High wind and solar regimes further increase the effect of rainfall variability and the whole complex makes a fragile ecosystem in which small disturbances may cause great loss to the sustainability, which are sometimes irreversible (Hou’erou, 1996). The hot arid zones of the world are economically and environmentally disadvantaged, with unique problems. The ecosystems of these zones are highly fragile with large risks that cause severe impediments to development programs. Of the total land area in the world, arid zones cover 18.8% and the hyper-arid zones account for 22.3% of the total arid zones (FAO, 1989).

In urban areas, there are spaces along the roads which can be beautified by planting various groups of plants by proper planning. In addition to that, there are large traffic islands in metropolitan cites which are ideal for developing gardens. Even narrow central verge of the two way roads provide ample scope for plantation. These kinds of plantations sometimes in the form of gardens are very useful and fulfill dual purposes - beautification and amelioration of environment.

Roadside Gardens

Scope - Vacant areas along both sides of the metal roads in semi-urban and urban places are ideal for plantation. Depending upon the space available, trees and shrubs can be planted in various ways.