eChapters

1. TYPES AND GRADES OF IMMUNITY

The immune response, which has inherited the character of memory, as evinced by rapidity and specificity of the responses on the second exposure to antigen, was cleared the minds of scientists especially bacteriologists who could find certain cells especially lymphocytes to play a major role in immune response.
 
The fact that the blood also contains soluble protective substances both heat stable which were later known as antibody and heat labile, later called as the complement system. These two were regarded as totally separate phenomenon in the beginning years. Eventually the distinctions between specific and non-specific responses were understood.
 
There are two types of immunity
    i)    Non-specific (Innate, natural or native) immunity
    ii)    Specific (Acquired) immunity.

A. Answer the following

Q.1.    What is the difference between apoptosis and necrosis?

Ans.    In apoptosis, there is pronounced decrease in cell volume, modification of cytoskeleton, condensation of chromatin and degradation of DNA into fragments. Due to these changes, an apoptotic cell sheds tiny membrane bound apoptotic bodies containing intact organelles. Macrophages phagocytose apoptotic bodies and cells in the advanced stages of apoptosis. But there is no release of proteolytic and the lytic enzyme, cationic proteins and oxidizing molecule in the surrounding tissue and no local inflammatory response. On the other hand, in necrosis cells swell and burst releasing their contents and possibly triggering a damaging inflammatory response.

Predominantly, an agrarian society is mostly dependent on the farm sector. Agriculture plays an important role in Indian economy notwithstanding its shrinkage in share in the gross domestic product (GDP) of the country being 55.4% in 1950-’51 and 17.4% in 2018-’19 (Tripathy, 2019). Still the agriculture and allied sector is providing employment to 54 percent of the Indian population.

1.1 Introduction

Poultry is one of the fastest-growing areas of India’s agricultural industry, with an annual growth rate of around eight percent. Over a span of four decades, the chicken industry in India has seen a paradigm shift in structure and operation, transforming from a simple backyard occupation to a significant commercial agri-based enterprise. Constant efforts in upgrading, modifying, and using new technologies opened the path for the multiplication and diversification of poultry industries. The expansion is not just quantitative, but also qualitative, sophisticated, and productive. This transition has necessitated substantial expenditures and increases in breeding, hatching, raising, and processing. The rise of the poultry industry in India is also characterised by the expansion of chicken farms.

To fully explore the history of medical and drug development, it would be necessary to go back as early as 13,000 BCE, where drawings on the Lascaux caves in France suggest that humans were using plants for medicinal purposes. Since then, medical traditions have evolved and dissolved across societies all over the globe. Natural products including plants, animals and minerals have been the basis of treatment of human diseases. The last two centuries have seen a focus on scientific medicine, with a focus on drugs whose effects that have been proved by the scientific method. Yet given this setting, many of the major pharmaceutical corporations have renewed their strategies in favor of natural product drug discovery. Numerous drugs have entered the international pharmacopoeia via the study of ethnopharmacology and traditional medicine. At this point in history, it is possible for both traditional and western medicine to benefit from collaboration between the two disciplines. For traditional medicines, newer guidelines of standardization, manufacture and quality control and scientifically rigorous research on the scientific basis for traditional treatments will be required. In return, traditional medical traditions can offer a more holistic approach to drug design and myriad possible targets for scientific analysis. Powerful new technologies such as automated separation techniques, high-throughput screening and combinatorial chemistry are revolutionizing drug discovery. Traditional knowledge can serve as powerful search engine, which will greatly facilitate intentional, focused and safe natural product drug discovery and help to rediscover the drug discovery process. By looking at the historical trends in drug and medical developments, it is possible to understand how current drug development will benefit from this partnership.

Metagenomics is a relatively new field that combines molecular biology and genetics in an attempt to identify, and characterize the genetic material from environmental samples and apply that knowledge. The genetic diversity is assessed by isolation of DNA followed by direct cloning of functional genes from the environmental sample. But more importantly, it is extremely beneficial in the study of synthetically made genomes. Metagenomics is described as “the comprehensive study of nucleotide sequence, structure, regulation, and function”. Scientists can study the smallest component of an environmental system by extracting DNA from organisms in the system and inserting it into a synthetic model organism. The model organism then expresses this DNA where it can be studied using standard laboratory techniques. The development of metagenomics stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent thevast majority of organisms in most environments on earth. This evidence was derived from analyses of 16S rRNA gene sequences amplified directly from the environment, an approach that avoided the bias imposed by culturing and led to the discovery of vast new lineages of microbial life.

Metagenomics is employed as a means of systematically investigating, classifying, and manipulating the entire genetic material isolated from environmental samples. This is a multi-step process that relies on the efficiency of four general steps as shown in the diagram. The procedure consists of: the isolation of genetic material, the manipulation of the genetic material, library construction, and the analysis of genetic material in the metagenomic library. Metagenomics provides a second tier of technical innovation that facilitates study of the physiology and ecology of environmental microorganisms. Novel genes and gene products discovered through metagenomics include the first bacteriorhodopsin of bacterial origin; novel small molecules with antimicrobial activity; and new members of families of known proteins, such as an Na⁺(Li⁺)/H⁺ ant porter, RecA, DNA polymerase, and antibiotic resistance determinants. Reassemblyof multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and micro heterogeneity, and lateral gene transfer among members of an uncultured community. The application of metagenomic sequence information will facilitate the design of better culturing strategies to link genomic analysis with pure culture studies.

Introduction There is an emergence of a transformative paradigm of participatory approaches in various fields such as management, health care and education, community development, etc. Participatory approaches challenge the top-down approach used frequently for decision making (Narayanasamy, 2009). Participation is a dynamic process where two individuals exchange their ideas, thoughts, and knowledge for making proper decisions and solving the problems faced by local people. It is a process of empowering individuals that helps people make proper analysis, make their own decisions, and develop their confidence. It is successfully used in increasing self-help activities of local people and for proper implementation and evaluation of large scale projects. FAO has reported that through participatory programmes it is very easy to mobilise local knowledge for self-reliant development, reducing government assistance costs.

Botanically fruits are ripened ovary. It is a plant part that may or may not be purely derived from ovary of the flowers may be fleshy or pulpy in character, often juicy, predominantly sweet. With somewhat acidic taste, fragrance, aromatic flavours, fruits are generally not consumed during principal part of meals. Whereas vegetable is plant part which is less sweet, generally non acidic and consumed either cooked or raw during the principal part of our main meals. Vegetables may be fruit, root, stem flower, or leaf part of a plant or their modification.
Fruits and vegetables are valued in in human diet as an important source of protective nutrients like Minerals and vitamins. Apart from their nutritive value they add variety to the diet and improve acceptability of meal due to presence of different shades of colours, flavours, and texture. However, the major role in food preservation is played by their moisture content. For the same reason they are classified as
1. Perishable: Those which deteriorate quickly after harvesting such as tomatoes, mangoes, papaya, peaches, plums, juicy fruits and vegetables. They have high degree of moisture content and are highly susceptible to spoilage.
2. Semi perishable: These have less moisture content as compared to perishable one’s example 30 carrots, peace, green beans, apple etc. These foods are containing certain natural inhibitors to spoilage.

What is seed?

“A seed is an embryo, a living organism securely fixed in the supporting or the food storage tissue.”

A true seed is defined as “Structurally a true seed is a fertilized matured ovule, consisting of an embryonic plant, a store of food and a protective seed coat, a store of food consists of cotyledons and endosperm”. In broad sense, “seed is a material which is used for planting or regeneration purpose”. Scientifically, “seed is a fertilized matured ovule together covered with seed coat is called seed” or “it is a propagating material i.e., part of agriculture, sericulture, silviculture and horticultural plants used for sowing or planting purpose”.

The business of Seed Technology is to protect this biological entity and look after its ‘welfare’, while the focus of Food Technology is on the second component – the supporting tissue.

Introduction Surimi and surimi based seafoods are traditional products of Japan and occupy an important position in the dietary culture of the country. Today, the largest producers of surimi are the United States, Japan and Thailand. Surimi is also manufactured in China, Vietnam and Malaysia. The process of makingsurimi originated in Southeast Asia and was further developed in Japan in the 16th century. Surimi is a product of Japanese origin, derived from a traditional Japanese way of using and preserving fresh fish. The word ‘surimi’ comes from the Japanese words ‘suru’ meaning ‘to process’ and ‘mash/mi’ meaning ‘meat’. Technically, surimi is the stabilized myofibrillar protein which is obtained by mechanically deboned fish flesh, which is washed, mixed with cryoprotectants, and stored frozen. Washing not only removes fat and undesirable matters such as blood, pigments and odoriferous substances but also increases the concentration of myofibrillar protein. A fish-based product serving the raw material for preparation of analog of seafoods like crab, lobster, scallop & other shellfish.

Thermochemical conversion is a process that uses heat to transform biomass and agricultural waste into energy and valuable byproducts. This approach involves the decomposition of organic materials under high temperatures, with or without the presence of oxygen, to produce fuels, chemicals, and thermal energy. The key thermochemical methods include combustion, pyrolysis, gasification, torrefaction, and hydrothermal liquefaction (HTL). In the pursuit of sustainable energy and environmental protection, biomass has emerged as a vital renewable resource. Thermochemical conversion technologies have gained prominence due to their high efficiency, rapid processing rates, and ability to handle a wide range of feedstocks—including lignocellulosic and low-moisture materials. Unlike biological processes, which depend on microbial activity and require longer processing times, thermochemical methods offer faster and scalable solutions suitable for industrial applications. Each thermochemical pathway offers unique advantages and outputs. Combustion is the most widely used method, involving the burning of biomass in the presence of excess oxygen to generate heat. This heat can be used directly or converted into electricity, making combustion especially common in rural and semi-urban settings. Other methods like pyrolysis and gasification produce intermediate products such as bio-oil, syngas, and biochar, which can be further refined or utilized as fuels and soil amendments. Hydrothermal liquefaction and torrefaction operate under specific conditions to produce high-energy-density products and improve biomass handling and storage characteristics. Overall, thermochemical conversion presents a robust strategy for managing agricultural waste while contributing to renewable energy generation and circular economy goals.
Pyrolysis, Gasification, and Hydrothermal Processing
Pyrolysis is a thermochemical process that involves the decomposition of biomass by heating it in the absence of oxygen, typically at temperatures between 300°C and 700°C. This process converts organic material into three main products:
1. Bio-oil (liquid)
2. Syngas (gas)
3. Biochar (solid)

Introduction

Parvoviruses are nonenveloped, single-stranded DNA viruses belonging to the genus Protoparvovirus, member of the Parvoviridae family, that has been included within the species Carnivore protoparvovirus 1, together with Feline panleukopenia virus (FPV), Mink enteritis virus (MEV) and Raccoon parvovirus (RPV), according to the International Committee on Taxonomy of Viruses (Tijssenet al 2011). Two distinct parvoviruses are known to infect dogs—the pathogenic CPV 2 and CPV-1 or the minute virus of canine (MVC). CPV-2, the causative agent of acute hemorrhagic enteritis and myocarditis in dogs, is one of the most important pathogenic viruses with high morbidity (100%) and frequent mortality up to 10% in adult dogs and 91% in pups. CPV-2 is the etiologic agent of a contagious disease, mainly characterized by clinical gastroenteritis signs in younger dogs. This virus that causes the disease “parvo”, first emerged among dogs in Europe around 1976. By 1978 the virus had spread unchecked, causing a worldwide epidemic of myocarditis and inflammation in the intestines (gastroenteritis) and could infect domestic dogs, spreading in the global dog population within 2 years. This original type, was rapidly replaced by two antigenic variants, types 2a and 2b (Parrish et al 1985, 1991), and in 2000 a third type CPV-2c was detected (Buonavogliaet al 2001). Canine parvovirus (CPV) is responsible for acute gastroenteritis in pups, with a high rate of mortality (Carmichael and Binn, 1981). We now know the virus is not limited to dogs, but is capable of causing infections in wild canines such as coyotes and wolves, and other wild animals, including foxes, raccoons and skunks. Since its emergence in 1978, it was and remains a common and important cause of morbidity and mortality in young dogs.

Introduction

The food problem in the world is expected to become more serious if the world population keeps growing at the present rate. Out of various strategies to solve the problem, the post-harvest handling of the grains is of paramount importance. Tribolium species is major pest of stored grain and grain products in the tropics. Control of this insect relies heavily on the use of synthetic insecticides, which has led to many problems such as increasing costs of application, pest resurgence, pest resistance to pesticides and lethal effects on non-target organisms in addition to direct toxicity to users and disturbances of the environment. Thus, insecticides of natural origin are rational alternatives of synthetic insecticides. Several botanicals, natural products and their derivatives have been reported and exhibit better efficacy and potency against the stored grain pests.

In the present investigation, an attempt has also made to test ovicidal, larvicidal and adult emergence suppression potentials of the extracts of some of the locally available Indian medicinal plant viz., Datura stramonium, Ricinus communis, Vitex trifolia and Nerium indicum against T. castaneum against T. castaneum, commonly called as Red flour beetle.

ABSTRACT

Papaya (Carica papaya L.) is an important crop in the tropics and subtropics for export of fruits and above all, for local consumption. Cultivation of papaya faces with problems due to its heterozygosity, dioecious nature and susceptibility to a large number of viral diseases. Crossing between two hermaphrodites will produce the highest ratio of fruit bearing female trees, which is of homozygous dominant andromonoecious types which are always letha l due to po st fertilization barriers leading to their abortion. The gynodioecious varieties of papaya have the M1M1 forms while selfing and hence for explant selection , gynodioecious varieties like Sunrise solo and CO7 were prefered Embryo culture is one of the earliest forms of in vitro culture applied to solve practical problems and is probably the tissue culture techniques that has proven of greatest value to breeders. Ovule culture is practised in a case when small (or) young embryos that abort at early stages of development and are difficult to isolate. This necessiates the development of a protocol for initiation, multiplication and regeneration of ovular callus of papaya.

? Oxalic acid is an organic dicarboxylic acid that readily forms insoluble salts with cations like calcium & magnesium.

? Sodium, potassium or ammonium salts are highly soluble and can induce toxicity in animals.

Source

? Ingestion of large quantity of oxalate containing plants.

? For toxicity plant should contain > 10% oxalic acid on dry weight basis.

? The form in which oxalate is present in plant mainly as acid oxalate (Oxalis sp., Rumex sp.) or oxalate ions (chenopodiacae).

Method I
Reagents
    1.    20% sodium carbonate.
    2.    Conc. hydrochloric acid.
    3.    4% sodium hydroxide
    4.    10% calcium chloride
    5.    Ammonium hydroxide
    6.    Potassium permanganate (0.05 N)

Procedure
Take 2-5 g of powdered sample in a beaker without spout, and add 40 ml of water and boil for half an hour. In case of faeces, add 40 ml of 4 percent NaOH instead of 40 ml of water to form silicates. Now boil, for half an hour with cold water flask over the beaker, then add 10 ml of 20 percent sodium carbonate solution and boil for  another half an hour. During these two cooking processes, the following changes take place.

7.1 INTRODUCTION

An understanding of the processes of oxidation and reduction in soils and the associated changes and practical implications requires a knowledge firstly of soil respiration and the movement of O₂ and CO₂ in soils, and secondly an appreciation of the significance of the changes which occur when, as a result of the use of all the O₂ in a given zone of the soil, respiration changes from aerobic to anaerobic. This results in marked changes in the biochemistry of the system. A basic knowledge is therefore needed of the microbiology and biochemistry of aerobic and anaerobic respiration and of the physics of gas movement, as well as of the chemistry of the system.

Introduction

Lipids are among the major components of food of plant and animal origin (food lipid). There is no precise definition available for the term lipid; however, it always includes a broad category of compounds that have some common properties and compositional similarities. Lipids are materials that are sparingly soluble or insoluble in water, but soluble in selected organic solvents like benzene, chloroform, ether, hexane, and methanol. Together with carbohydrates and proteins, lipids constitute the principal structural components of tissues (Erwin Wsowicz et al., 2004). Lipid oxidation is one of the major reasons that foods deteriorate and is caused by the reaction of fats and oils with molecular oxygen resulting in off-flavours that are generally called rancidity. Exposure to light, pro-oxidants and elevated temperature will accelerate the reaction (Velasco, J et al., 2010). Rancidity is related to characteristic off-flavour and odour of the oil. There are two major causes of rancidity. One occurs when oil reacts with oxygen and is called oxidative rancidity (Ribeiro et. al., 1993). The other cause of rancidity is by a combination of enzymes and moisture. Enzymes like lipases liberate fatty acids from the triglyceride to make di and monoglycerides and free fatty acids and such liberation of free fatty acids is named hydrolysis. Hydrolysis is additionally caused by chemical process that’s prompted by factors like heat or presence of water. Rancidity caused by hydrolysis is called hydrolytic rancidity. Oxidation cares mainly with the unsaturated fatty acids. Oxidative rancidity is off interest because it results in the event of unfavourable off-flavours which will be detected early within the development of rancidity, more so than in the case of hydrolytic rancidity. Oxidation can alter the flavour and nutritional quality of foods and produce toxic compounds, all of which may make the foods less acceptable or unacceptable to consumers. Oxidation products typically include low relative molecular mass compounds that are volatile also as undesirable off-flavour compounds (Food lipids).

Introduction

The present trend in the search for new drugs is to look at natural sources, which are safer for humans and ecofriendly. Of late there has been increased awareness about the detrimental effects of synthetic food supplements, food additives and drugs, and the search for natural alternatives in plants has intensified. In this regard, horticultural crops are particularly appealing since they are not only edible, but also have diverse nutraceutical effects. Nutraceuticals (often referred to as phytochemicals or functional foods) are natural, bioactive chemical compounds that have health promoting, disease preventing or medicinal properties (http://foodsci.rutgers.edu),such as antioxidant, anti-inflammatory, hypoglycemic, hypolipidemic, antimicrobial effects, to name just a few. Most of these properties are attributed to the secondary metabolites present in plants, various phytochemicals including flavonoids, terpenoid, lignans, sulfides, polyphenolics, carotenoids, coumarins, saponins, plant sterols, curcumins, phthalides etc. The medicinal value of our horticultural crops is not a new knowledge, it has been exploited by the traditional systems of medicines like Ayurveda, folklore medicines and ‘kitchen remedies’ since time immemorial.

A functional food is similar in appearance to, or may be, a conventional food that is consumed as part of a usual diet, and is demonstrated to have physiological benefits and/or reduce the risk of chronic disease beyond basic nutritional functions, i.e. they contain bioactive compound.

A nutraceutical is a product isolated or purified from foods that is generally sold in medicinal forms not usually associated with foods. A nutraceutical is demonstrated to have a physiological benefit or provide protection against chronic disease.

2.1. Introduction
The present century is the century of oxidative stress (OS) that linked to several life-threatening disorders which predominantly affect public health as well as their nutritional status. Unhealthy lifestyle and eating pattern which is major concern for OS. The biological literature first used the phrase “oxidative stress (OS)” in 1970. OS can be defined as a state of imbalance condition between the high level of pro-oxidants and the low level of antioxidant scavenging capacity in the body. The normal oxidative process that occurs in cells is crucial for the existence of life and favours the death of a cell. Free radicals, which are unstable, can form when molecular oxygen breaks up into pairs. This unstable radical has a high level of reactivity and produces reactive molecular species like reactive oxygen species (ROS) and reactive nitrogen species (RNS). To resist the OS and to maintain the redox balance, cells have an inbuilt antioxidant defence capacity, comprising of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPOD) etc. But some pathogenic or environmental conditions may overpower this antioxidant defence system, allowing a portion of reactive oxygen and nitrogen species (RONS) to bypass degradation and create much more reactive radicals. Increased ROS and RNS affect redox balance and damage DNA, proteins, amino acids, and fats, causing alteration of structures as well as action of cells and tissues. This will ultimately produce several pathogenetic elements associated with clinical situations and leads to the development of human aging and numerous diseases. Several dietary components such as whole grains, nuts, fruits, and vegetables are excellent sources of antioxidative vitamins, minerals, nutraceuticals or phytoceuticals that shields the human body from the harmful oxidative injury by scavenging or destruction of free radicals and also by upregulation of endogenous antioxidant defence system. Thereby long-term and sustainable OS remedies can be performed.