eChapters

The floriculture has become an important sector all over world. In India, floriculture has been associated with culture and heritage since very ancient time. In recent decades there has been increasing in demand of floriculture products with increasing income. It is souring industry in Asian countries including India. Floriculture is an emerging area with great potential both in the domestic as well as export market.In India, commercial floriculture is ongoing development but have a long tradition of various types of flowers.Floriculture was being cultivated in 3.5 lakh hectares in the country and 250 varieties of flowers exist in the country.Besides potted plants, roses, carnation, orchids and chrysanthemum are important cut flowers, which are popular in our country. In the increasingly competitive international cut flower and pot plant market, new and novelty ornamental plants plays an important role in stabilization and expanding market share. Always novelty has been considered as an important attribute in ornamental industry. The consumer choice is also changing for product quality level depending upon purpose of purchase, as well as for higher levels of services and a wider and deeper assessment suggesting product diversification. The increasing pace of agricultural transformation in India has been characterized by widening share of commercial crops in the overall basket. Among others, horticulture has shown great promise because of the availability of high-value domestic and international market against the low production base so far. Understandably, it has received special attention of policy-makers and being termed as the sunrise industry within agriculture. India, with its varied agro-climate is ideally suited for growing several types of flowers in different seasons. Although traditional ornamental crops will continue to play key role in the floriculture trade, a distinct trend towards increasing the share of new crops is clearly evident in recent years often referred to as ‘specialty flowers’, these are cut flowers, which are not widely known and also grown in small pockets but have high prospects in the international market. There are over 60 types of new ornamentals, which have been reported to hold potential for commercial utility. Some progressive growers in India have already initiated the process of introducing new ornamental crops in their fields.These new products normally fetch high prices than the traditional crops for a certain period, but quite often the prices drop when market is saturated. By that time new products should be ready to enter the market. In this context introduction of new ornamental crops is therefore continuous effort.

India has emerged as the second largest producer of vegetables 176.17 mt after China 573.93 mt in the world and is gradually striding towards achieving food and nutritional security at national level. The average productivity of vegetable crops is 17.6 t/ha (NHB, 2015) which is comparatively lower than the productivity of China (23.4t/ha), Spain (39.3t/ha) and USA (32.5t/ha). The lower productivity could be attributed to limited use of quality seeds, inefficient production management, impact of biotic stress and climatic factors during crop production. Among the limiting factors limited availability of quality seed is one of the important factors. Since, the performance of other inputs is also depend on the quality of seed. At present the seed replacement rate among vegetable crops is around 25%, whereas highest in cabbage (>90%) followed by tomato, capsicum, bitter gourd, muskmelon, bottle gourd etc.The use of hybrid seed is in increasing due to higher productivity, tolerance to biotic and a biotic stresses and better market value than the open pollinated varieties but the higher price of the hybrid and their availability is still a constraint in adopting the use of hybrid seeds among small and marginal farmers.

India has made a considerable progress during the last three decades in horticulture production in India producing 300.6 million tonnes of horticultural produce from 24.85 million hectares in 2016-17. The average productivity of different horticultural crops is quite low as compared to the best yields at national and world level, indicating the scope to enhance the yield in different horticultural crops by many fold along with its quality specially in vegetables. Unlike vegetables, flower cultivation is growing at a very fast speed and registered a marked growth along with employment generation through protected cultivation. According to NHB (National Horticulture Board), during the year 2014-15 the area under floriculture production in India was 248.51 thousand hectares with a production of 1,658 thousand tonnes loose flowers and 484 thousand tonnes cut flowers. However, India’s total export of floriculture was Rs. 548.74 crores/ 82.05 USD Millions in 2016-17. This is because of an appealing gap between potential yield and the actual yield of various vegetable crops. In many vegetable crops, the yields realized are even less than 50 % of their potential yield. Due to fluctuating market prices and poor storage infrastructure available for horticultural crops, farmers are not able to get their production cost. Though, there are some crops, fetches high prices during off-season. Several biotic and abiotic stresses are major factors, responsible for low productivity and poor quality in several horticultural crops under their open field cultivation during rainy and post rainy season more specifically, in vegetables. The increasing demand of off-season and high quality vegetables in various markets of the big cities has drawn the attention of the vegetable growers for diversification from traditional system.

Sugarcane is the most important sugar crop contributing more than 62% of the world's sugar production. With increasing human population in the world, sugar requirement has also gone up. Molasses is an important by-product of sugar industry. It is used for the production of alcohol by the distillaries. Alcohol serves as a base material in pharmaceuticals and several other industries. The manufacture of gur (jaggery) and khandsari as alternative sweetening agents in India from sugarcane also plays considerable role in the Indian economy, particularly in rural area. Nearly 40 % of the sugarcane produced is utilised for manufacture of gur and khandsari. Sugarcane is bound to play a greater role in the Indian economy in the years to come by offering a stable income to the farmers by way of cane price, by providing employment to rural masses, by realising excise duty, Cane Cess/ purchase tax and foreign exchange earning.

Acreage and production

Sugarcane is grown in tropical and sub tropical regions. Brazil has largest area and production of sugarcane.India is second to Brazil in sugarcane production. It is followed by Cuba and China. Other important cane growing countries are Pakistan, Mexico, South Africa, Australia, Indonesia etc. So far, as average sugarcane productivity is concerned Peru ranks first in average sugarcane yield. To meet the sugar requirement of increasing population in future, it is essential that sugarcane production is stepped up. Since there is no scope at present to bring additional land under sugarcane, productivity of sugarcane, the targets have to be achieved only through increased productivity.

Rice is the most important crop in India occupying about 43.7 million hectares area with a production of 136.5 million tonnes (rough rice/ paddy). As the human population is increasing, so is the demand for rice, yet yields of the crop are leveling out. At present, the rice yield in India is 3.12 t/ha as against the world average of 4.11 t/ha. Not only this, the rice yield in India is exactly half than that of China (6.26 t/ha). The World Development Report 2008 predicts that cereal production would have to increase by 50% by 2030 to meet the escalating worldwide demand. The additional rice for increasing population will have to be produced on less land with less water, less labour, and reduced inputs.The major constraint in rice production in India is the lack of suitable crop management practices and sufficient irrigation facilities. The water crisis is threatening the sustainability of the irrigated rice system and food security in many parts of the world. Despite efforts on a variety of fronts, including genetic engineering of rice strains for improved yield and nutrition, no concrete solution has been found yet. There is a challenge to develop novel technologies and production systems that allow rice production to be maintained or increased in the face of declining water availability. To produce 1 kg of grain, farmers have to put 2 to 3 times more water in rice fields than in those growing other cereals.

The increasing water need for agriculture poses a potential threat to natural water resources and climatic change scenarios in the near future. The great challenge will be to increase food production with less water, particularly in countries with limited water and land resources, by promoting techniques and cropping systems of higher water-use efficiency (WUE).There is also much evidence that water scarcity already prevails in rice-growing areas in India, where rice farmers need technologies to cope with water shortage and ways must be sought to grow rice with less water.The farmers, especially those, who are resource-poor, are losing interest in rice cultivation as its profitability is declining with the rise in input costs. But, system of rice intensification (SRI) and aerobic rice culture appear to be a viable alternative of rice cultivation that may save the expensive inputs, improve soil health/ quality and protect the environment substantially.

Introduction

Lentil is one of the important Rabi pulses. It is one of the oldest pulses crops and the most nutritious of the pulses. It has the potential to cover the risk of dryland agriculture. It is also used as a cover crop to check the soil erosion in problem areas. It is mostly eaten as ‘dal’. Lentil can also be used as green fodder. Its straw is also used as cattle. Being a leguminous crop, it restores the soil fertility.

Area of cultivation

Lentil is mostly cultivated in the northern and central parts of India. The important lentil growing states are Madhya Pradesh, Uttar Pradesh, Bihar, West Bengal, Orissa, Haryana, Rajasthan, Punjab and Assam. Uttar Pradesh and Madhya Pradesh together contribute 78% towards total national area as well as production.

Climatic requirements: Lentil requires cold climate during growth and warm temperature at the time of maturity. It can tolerate cold and frost winter up to great extent. The optimum temperature for growth is 18-30 degree centigrade. Excessive drought and/or high temperatures during the flowering and pod-fill period also reduce yields.

Soil: Lentil can be grown on a variety of soils such as light loams; alluvial soils and black cotton soils. This crop is also suited to the poorer types of soils, low lying areas such as paddy fields. Lentil does not tolerate flooded or waterlogged soils. Good drainage is required, because even short periods of exposure to waterlogged or flooded field conditions kill plants. A soil pH near 7.0 is best for lentil production. Acidic soils are not fit for growing lentil.

India ranks third after China and Canada in the world in production of edible oilseed crops. Average yield of rapeseed-mustard in the world is 2043 kg/ha. Average yield of European Union, Canada and China is higher than India. Higher yield in these countries is due to cultivation of long duration varieties suitable for their local climatic conditions. Duration of favorable climatic condition to grow rapeseed-mustard is shorter in India as compared to EU, Canada and China, which enforces cultivation of short duration varieties (130-145days). Rapeseed-mustard contribute one third in the production of edible oils in India. These crops ranks second in area and production after soybean. Total area and production under these crops is 5.80mh and 6.28mt respectively. It is necessary to mention that total water requirement of these crop is 80-240mm, which is very low as compared to other crops. These crops pay higher return by utilizing low inputs, thus, are highly remunerative for the farmers.

Rapeseed-mustard group of crops are mainly cultivated in irrigated areas where at least one irrigation facility is available and only 24.0% of area is under rain-fed situation. Five states of India namely, Rajasthan, Uttar Pradesh, Haryana, Madhya Pradesh and West Bengal contribute 82.00% and 85.67% in total area and production, respectively, under these crops. In Madhya Pradesh 53.2% of total area is irrigated with average yield of 1006 kg/ha, however, 76.6% of total area of country is irrigated even then average yield is only 1083 kg/ha. The lower average seed yield can be attributed to (i) cultivation of very short duration and low yielding B. rapa genotypes in Eastern and North-Eastern states; (ii) susceptibility of varieties to different pests; and (iii) high temperature stress at seedling and reproductive stage. Out of total area under these crops, Indian mustard (Brassica juncea) is grown over more than 80% area. Cultivation of Indian mustard has also been taken up in non-traditional areas of southern states of India viz., Karnataka, Tamilnadu and Andhra Pradesh, recently. This has become possible due to its higher average yield, tolerance against different biotic and abiotic stresses, availability of varieties with 100-152 days maturity, and non-shattering behaviour.

Rice is the most important cereal crop of India occupying about 44.0 million hectares land. It provides food and employment to millions of people in the country. Despite development of high yielding varieties and technological interventions the yield of rice is still low (3.20 tonnes/ha) in India. The reasons for low yield of rice are:

Lesser area under high yielding varieties

Limited availability of quality seed

Increased acidity/ sodicity in some parts of the country

Declined soil fertility

Unavailability of inputs, like fertilizers and seed, in time

Inefficient nutrient management including imbalanced use of nutrients

Improper crop rotations

Reduced availability of irrigation water

Inefficient management of weeds, diseases and insect-pests

Introduction

Agriculture occupies a prominent place in Indian economy and rural livelihoods. But, it is faced with the challenge of producing food for a rapidly growing population whilst maintaining the nation’s fragile resources.Efforts through green revolution brought out significant increase in food grain production by concentrating on the potential farms in terms of irrigation and other basic resources.Nationally and globally, it is necessary that the growth rate of food production must remain higher than the population growth rate to avoid hunger and overcome famines. But, food security one of the basic human rights is in jeopardy, today. Agriculture is crucial for ensuring the food and livelihood security of the country and hence it is important that this sector with diversified farming systems becomes resilient to increasing climatic variabilities and global economic changes. This concept encourages the farmers towards efficient utilization of natural resources and also proper channelization of by-products of one enterprise to the other enterprise in farming. The integrated farming systems, therefore, assume prime place for sound management of farm resources to enhance the farm productivity, reduce the environmental degradation, and improve the quality of life of resource poor farmers and to maintain the sustainability to meet the challenges of food security. In order to meet the soaring demand for food there is a need to go for integrated faming system.This approach facilitates in strengthening the socio-economic status of Indian farmers besides strengthening ecological balance. Integrated farming system is common to Indian farmers simply because of the preponderance of small farm systems which form the backbone of traditional agriculture. For small farmers, these systems enable a means of diversifying the use of meagre resources in the context of a rational means of reducing risk. Additionally, it also enables increased efficiency in the use of these same resources in a manner that there will be livelihood security and stability to farming systems. There is no scope for increasing the farm size. Yet steady increase in population with shrinkage of cultivated land as a result of industrialization and urbanization would pose serious hurdle for agricultural development. Only vertical expansion is possible by integrating appropriate farming components requiring lesser space and time ensuring continuous income to the farmers.

Microorganisms live as single cells in nature in association with other cells in assemblage that we call “Population”. Such populations are composed of group of related cells generally derived by successive cell divisions from a single parent cell. The location in an environment where a population generally lives in association with other populations of cells called “Microbial communities”. Environmental microbiology deals with the study of these microbial communities in different habitats, naturally occurring on earth.

Natural habitats of microorganisms are exceedingly diverse. Any habitat that is suitable for the growth of microorganisms can also support the growth of other microorganisms. But in addition, there are many habitats where, extreme environment, due to chemical and physical factors, higher organisms cannot grow but microorganisms can grow and flourish. In this chapter, different habitats of microorganisms are discussed briefly.

Because microorganisms are so small, their habitats are also small. The term microenvironment is often used in ecology to describe where a microorganism actually lives and metabolites within its habitat.

Development of sophisticated procedures for the isolation, manipulation and expression of genetic materials is a field called genetic engineering. Genetic engineering has applications in both basic and applied research. In basic research, genetic engineering techniques are used to study the mechanisms of gene replication and expression in prokaryotes, eukaryotes and viruses. For applied research, genetic engineering used for development of microbial cultures capable of producing valuable products such as human insulins, growth hormones, interferons, vaccines, enzymes etc. The commercial application of genetic engineering is sometimes referred as Biotechnology. In broad sense, Biotechnology refers the use of living organisms to carry out defined chemical processes for industrial application.

The process of isolation, purification and replication of specific DNA fragment is called molecular cloning or gene cloning. Molecular cloning is the base of most of the genetic engineering procedures. The purpose of molecular cloning is to isolate large quantity of specific genes in pure form. Thus molecular cloning is very important in genetic engineering and can be divided into several steps depends on the purpose. The steps are as follows:

The pathogenicity of an organism varies form mild attack to severe fatal attacks. The host has many mechanisms to resist or to avoid the pathogens apart from immune systems. One or few of these will be activated when the pathogen enters into the body. Following are some of the listed natural host defense mechanisms.

Industrial Microbiology is the discipline that uses micro organisms, usually grown on large scale, to produce commercial products or carry out important chemical transformations.

The products derived from such processes are

1 Alcohols and alcoholic beverages.
2 Production of pharmaceutical agents like antibiotics, vaccines etc.
3 Food additives
4 Enzymes
5 Chemicals
6 Single cell proteins

Genetics is the study of what genes are, how they carry information, how their information is expressed, and how they are replicated and passed to subsequent generations or other organisms.

DNA in cells exists as a double-stranded helix; the two strands are held together by hydrogen bonds between specific nitrogenous base pairs: A-T and C-G.

A gene is a segment of DNA, a sequence of nucleotides, that code for a functional product, usually a protein.

When a gene is expressed, DNA is transcribed to produce RNA; mRNA is then translated into proteins. This is also referred as Central Dogma of Life. The DNA in a cell is replicated before the cell divides, so each daughter cell receives the same genetic information.

Sterilization refers the complete removal or elimination of microorganisms from an environment. Physical and chemical agents are available to remove the microorganisms from the environment. Heat, irradiation and filtration are the three physical agents and various chemicals are available for sterilization.

Terms related to control of microorganisms

Cide or cidal means to kill the microorganism (Ex. Bactericidal refers the killing of bacteria).

Static or stasis means inactivation or inhibition, but not killing. They prevent multiplication of the organism. (Ex. Fungistatic refers the inhibition of growth of fungi.).

Sepsis refers break down of a living tissue by organisms and is accompanied by inflammation and pus formation.

Antispetic is an agent applied externally on living tissues to kill or inhibit the growth of the organisms.

Disinfectant is an agent applied externally on inanimate objects (not living tissues) to destroy harmful pathogens in their vegetative stage.

(Antiseptics are milder than disinfectants because to avoid the side effects)

Sanitation refers removal of organisms from a location by cleaning but not by sterilization.

The essential nutrients and their function in microbial cells

Virus can be defined as a genetic element containing either DNA or RNA that replicates in host cells as intracellular parasites but is characterized by having an extracellular state.

Virus can use the metabolic machinery of the host cell and can modify the genetics of the host cell. Virus has both extracellular and intracellular state.

Extracellular State

Outside the host cell, the virus is a minute particle containing nucleic acid surrounded by protein, which is referred as virions or virus particles. Virions are inert and have no biosynthetic and metabolic functions. Extracellular state or virus particle is the perfect stage of virus to study the morphology and chemistry.

Intracellular State

The active state in which the virus replicate in the host cell. When the virus nucleic acid is introduced to host cell and replication starts, the process is referred as infection. The cell in which infection of virus and replication takes place is referred as host cell.

Proto – first; Zoon – animal

Protozoa are single celled eukaryotic organisms, which lack cell wall and ability to move at some stage of their life cycle.

They are referred as the first animals.

There are about 65000 described species grouped into 7 phyla. Among them, 50 per cent are not available as living forms (as fossils) and out of remaining 50%, 22,000 species are free-living and 10,000 species are parasitic forms.

The protozoa are distinguished from other organisms by following characters

Algae are a large and diverse group of eukaryotic organisms that contain chlorophyll and carry out the oxygenic photosynthesis.

Study of algae is referred as phycology or algalogy

They vary from cyanobacteria or blue green algae by their eukaryotic nature.

Eukaryotic, spore – producing, achlorophyllous organisms with absorptive nutrition that generally reproduce both sexually and asexually and whose thallus is usually filamentous, branched somatic structures known as hyphae, typically surrounded with cell wall.

Mycology – Study of fungi

The fungi can be divided into two groups namely

True fungi: Chytridiomycota, Zygomycota, Ascomycota, Basidiomycota

Additional phyla: Myxomycota, Dictyosteliomycota, Acrasiomycota, Plasmodiophoromycota, Oomycota, Labyrinthulomycota, Hypochitridomycoya.