eChapters

Insecticide resistance is a growing concern for those who need insecticides for medical, veterinary, and agricultural control of insect pests. In response to the introduction of DDT resistance in 1947, the incidence of resistance has increased annually by an alarming amount. Resistance is “the inherited ability of a strain of some organism to survive doses of a toxicant that would kill the majority of individuals in a normal population of the same species” (WHO, 1957).

As a result of continued insecticide application, the proportion of resistant insects increases compared to that of susceptible and the population becomes increasingly difficult to control

Above photograph theoretical example illustrating the increase of insecticide resistance levels in a pest population. Some individuals (black) with genetic traits allowing them to survive insecticide applications can reproduce; if the selection pressure is frequent, they easily become the preponderant part of the population (Fig. 1).

An adaptation to a pesticide leads to reduced susceptibility to it among the pest population targeted by that pesticide. In nature, pests develop resistance to chemicals through natural selection. In the most resistant organisms, their genetic traits are passed on to their descendants. Since 1945, at least 500 species of pests have developed resistance to pesticides (Anonymous, 2007), but other sources estimate as many as 1000 species (Miller, 2004).

Synergists are chemicals that significantly increase the toxicity of an insecticide while being nearly non-toxic on their own. Thus, non-toxic chemicals that contribute to the increased toxicity of insecticides are referred to as synergists. Many chemical substances have the potential to synergize with poisons. Some enzyme inhibitors work well synergistically by inhibiting enzymes that metabolize pesticides, particularly in insecticide-resistant strains. When mixed with other insecticides, some insecticides increase the toxicity of the latter at non-toxic dosages. When a mixture is more harmful than expected based on the sum of its separate efficacies when administered alone, the interaction between substances is known as synergy. Antagonistic interactions among components reduce a mixture’s efficacy.

Synergism can also develop between two or more poisons. Several statistical methods have been proposed to quantify the synergy of both non-toxic and hazardous substances. Toxin interactions are assessed using bioassays, which involve mixing toxicants (two or more) in a specific ratio. The combination is then used to prepare serial dilutions. Alternatively, the poisons can be supplied sequentially in the same amounts as in the mixture. Bioassays are also used to determine the dose-mortality response of each toxin on the same insect strain. The data are subjected to probit analysis, and the toxin interactions can be evaluated using the statistical methods listed below.

Insecticide mode of action is divided in to four major chapters 1. Nerve & Muscle 2. Growth & development 3. Respiration & Energy production 4. Metabolic process 5. Unknown or Non-Specific (multisite inhibitors) (Source: IRAC). To understand the mode of action of insecticides, it is necessary to understand the function of nervous system and how it works in insect. The nervous system functions transmits information throughout the body. This system has three components: 1) Peripheral nervous system (PNS) to receive and transmit incoming signals (taste, smell, sight, sound, and touch) and to transmit outgoing signals to the muscles and other organs, effectively communicating them how to respond, and 2) Central nervous system (CNS) to interpret the signals and coordinate the body’s responses and movements. 3) Visceral or sympathetic nervous system (VNS) directly connected with the brain that supplies nerves to the anterior part of the alimentary canal (foregut and midgut), heart, and spiracles. Some additional nerves arise from posterior compound ganglion of ventral nerve cord (VNC) which supply nerves to the posterior part of gut and reproductive system.

Pesticide use in agriculture has evolved at several stages in the last few decades. Pesticides, as defined by the FAO, are any substance or mixture of substances intended to prevent, destroy, or control any pest, including vectors of human or animal disease, unwanted species of plants or animals causing harm during or otherwise interfering with the production, processing, storage, transport, or marketing of food, agricultural commodities, wood and wood products, or animal feedstuffs, or substances that may be administered to animals. A plant growth regulator, defoliant, desiccant, or agent used to thin or prevent premature fruit fall, as well as substances applied to crops before or after harvest to prevent them from deteriorating during storage and transportation, are included in this category.

Today’s agrochemistry has a massive challenge: ensuring the availability of high quality active ingredients to enable long-term control of pest species, weeds, and agricultural diseases. The differential uptake and transfer of the active ingredient by target and non-target organisms can contribute to diverse biological activity.

The way a chemical moves, persists, and behaves in its surroundings is known as its behaviour. A chemical’s behaviour will dictate both its residual properties and field efficacy. The behaviour of insecticides is determined by their chemical and physical characteristics as well as the interaction of environmental elements. Our capacity to comprehend and forecast how insecticides will behave in the environment will determine our ability to keep utilizing insecticides.

Important features of systemic insecticides

1. They are not susceptible to wash off by rain

2. By virtue of their movement inside plants, they can afford to protection to regions where they have not been sprayed

3. They are often been transported towards region of growth

4. Since they have very weak contact action, safe to beneficial organism.

Insecticide classification majorly divided into three categories. A. based on chemical group, B. mode of entry in the insect, C. based on toxicity.

A. Classification of Insecticides based on chemical group

Historians have traced the use of pesticides to the time of Homer around 1000 B.C. earliest records of insecticide use pertain to the burning of “brimstone” (Sulfur) as a fumigant. Pliny the Elder (A.D. 23-79) recorded most of the earlier insecticide uses in his Natural History and included among these was the use of gall from a green lizard to protect apples from worms and rot. Later, a variety of materials used viz., extracts of pepper and tobacco, soapy water, whitewash, vinegar, turpentine, fish oil, brine, lye and many others. At the beginning of World War II (1940), insecticide selection was limited to several inorganic compounds, arsenicals, petroleum oils, nicotine, pyrethrum, rotenone, sulfur, hydrogen cyanide gas, and cryolite. It was during World War II that opened the Chemical Era with the introduction of a totally new concept of insect control.

Introduction

Analysis of insecticide formulations are critical for the availability of quality insecticides in the market for ultimate benefit of farmers and ecosystem health. This chapter highlights the significance of comprehensive insecticide formulation analysis protocols, encompassing methods for determining active ingredient and assessing physical and chemical parameters. Employing advanced analytical techniques such as spectroscopy and chromatography for insecticide formulation analysis will eventually maintain the quality of insecticides for the sustainable management of pests.

In India, over 339 pesticides are registered that includes insecticides, fungicides, herbicides (DPPQS, 2024). The current market of non-genuine insecticide is INR 3,200 crores (USD 525 Million) which constitutes 25 per cent by value and 30 per cent by volume of the total domestic market of agrochemicals in India as per Industry reports, primary interviews, news articles and Tata Strategic analysis (FICCI, 2015). Monitoring registration and regulation of insecticides is governed by the Insecticide Management Bill 2020, which replaced the Insecticide Act 1968 (DPPQS, 2023). The Insecticide Act of 1968 established the Central Insecticide Laboratory, Faridabad, under section 16 with two regional insecticide testing laboratories at Chandigarh and Kanpur (DPPQS, 2023). Current Quality Control of Insecticides in States/UTs during the last five years shows that 68078 insecticide samples were analysed (DPPQS, 2023). In the beginning, these laboratories involved in analysing insecticide samples drawn by any officer or the body authorized by the Central or State Governments and submit certificates of analysis to the concerned authority. Regional Pesticide Testing Laboratories (RPTLs) had a huge target of analysis of 1550 samples per annum that necessitated the establishment of 71 State Pesticide Testing Laboratories (SPTL) spread across India of which 14 are NABL accredited (DPPQS, 2023).

Formulation is a word that can be used in a lot of different ways, but at its core, it means putting things together in the right way, usually by following a formula. Insecticides come in different forms, which are called formulations. A formulation is just the way a product is used. The chemical or biological makeup of a pesticide and its active ingredient (also called the active substance) determine how it works on living things. Most of the time, the active ingredient is mixed with other things, and the product as it is sold will need to be weakened even more before it can be used. Formulation makes a drug easier to handle, store, and use. It can also have a big effect on how well it works and how safe it is.

Insecticides come in many different forms because the active ingredients vary in how easily they dissolve, how well they kill pests, and how easy they are to handle and move. The pesticide is made up of both active and non-active (used to be called “inert”) chemicals. The manufacturer will need to know the identity of the pest, as well as details about its feeding behavior, reproduction, and life cycle, in order to create an effective formulation. Manufacturers also take into account factors including surface and equipment type, runoff and drift rates, pest habits, and safety when formulating insecticides. Inactive materials (adjuvants/ additives) are often added to insecticides to make them stick to the application surface or spread out over leaves. There are solvents (liquids) that dissolve the active ingredient, carriers (liquids or solids) that aid in the delivery of the active ingredient, etc.. Boosting the efficacy of active ingredients requires additives, such as spreaders, stickers, wetting agents, compatibility agents, foaming agents, etc.

A bioassay, also known as a biological assay, is a scientific experiment used to quantify the impact of a substance on a living organism. According to Finney (1952), bioassay is defined as measurement of potency of any stimulus (physical, chemical, biological, physiological) by means of reactions which it produces in living matter. It is an essential procedure in insecticide development and the assessment of environmental pollutants, as well as the evaluation of the effects of interventions viz. pesticides and herbicides on the environment. In the field of biostatistics, a bioassay refers to a set of techniques used to determine the quantity or intensity of an agent or stimulus based on the response of the subject. Typically, many methods are employed to assess the strength or effectiveness of an insecticide by examining its impact on living organisms.

An insecticide is a pesticide used to kill or eliminate insect pests in agriculture, households, and industries. Judicious use of insecticides may be a factor in the increase of agricultural productivity, but by their nature of having high toxicity to non target organisms and capability to develop resistance through widespread use, most insecticides have high potential to significantly affect and alter ecosystems. Many are toxic to humans and animals (both domestic and wildlife), and can accumulate as concentrates in the food chain and water resources, giving rise to serious environmental contamination and pollution.

History of Chemical Control, Insecticide Toxicology and Properties

History of pest control probably started when the first person swatted a mosquito or pulled off a tick, the battle for the control of our planet didn’t start until organized agriculture, when pests attacked the plants, and we grew to eat and threatened our very survival.

A pesticide is any substance or mixture of substances used to stop, kill, avoid, or lessen the effects of a pest (insects, mites, nematodes, weeds, rats, etc.). This includes insecticide, herbicide, fungicide, and many other substances used to control pests. Pesticide was defined in different ways at different times and in different places. But pesticide is still generally the same: it is a (mixed) substance that is poisonous and effective on target organisms but safe for non-target organisms and environments.

Chemical control, commonly known as pest control, is the use of chemicals to reduce insect populations. Herbicides, fungicides, insecticides, rodenticides, acaricides, nematodes, and fungicides are all examples of pesticides. Insecticides are a class of chemicals used to combat insect pests. A substance or combination of compounds used to kill, repel, or otherwise prevent insects is called an insecticide. When it comes to controlling pests, insecticides are most powerful tools available for management. They have a wide range of positive effects, including high efficiency, speed of healing, general applicability, adaptability to different settings, adaptability to shifting agronomic and ecological conditions, and low cost. When insect pest numbers approach or surpass the economic threshold, insecticides are the sole viable instrument for emergency action in pest management. The usage of pesticides, a widely used method, can be central to such a system. The use of chemical pesticides will remain pivotal in these initiatives. In many cases, chemical control of pests is the best option available. Some people’s view of pesticide use for pest management as an ecological sin is incorrect. Chemical pesticides are a reliable and useful tool for the biologist, so long as they are used in accordance with good ecological principles.

Mercury, a heavy metal, is present vi. Various forms and is well known for its toxic effects especially on human health. Mercury occurs naturally vin the environment through volcanic eruptions, weathering of rocks and forest fires. It can be observed in soil, water and the atmosphere. Human activities such as mining, industrial processes (eg. Coal Combustion, chor alkali production) and improper disposal of mercury containing products contribute to environmental contamination. Mercury can be absorbed through inhalation ingestion or skin contact. Once absorbed, mercury distributes throughout the body, crossing g the blood brain barrier and placenta. Inorganic mercury is partly metabolized to organic forms, such as methyl mercury, by micro organisms in environment. Mercury is excreted via Urine and feces. Mercury disrupts neuronal function by interfering with neurotransmitter signalling pathways, cognitive function, motor coordination and sensory perception Mercury accumulates in the kidneys, causing tubular necrosis. Proteinuria and renal failure. Clinical signs and symptoms are tremors memory loss, nausea, vomiting, hypertension and chest pain. Diagnosis is based on medical history, physical examination, blood, Urine, hair tests and neuro imaging techniques namely MRI or CT scans. Treatment is based on chelation therapy. Finally it is concluded that understanding the sources, toxico kinetics, toxico dynamics clinical manifestations and treatment options is critical for effective management and prevention of mercury toxicity.

An auricle (pinna) and ear canal (external auditory meatus) of external ear play an important rolein the process of hearing. The main function if external ear is to collect vsound waves and divert them towards the middle and inner ear. The external ear I'd prone to different conditions along with inflammation, infections and other disorders. Common conditions of the external ear include otitis external, Carmen impaction. Contact dermatitis, external otitis media. Antibiotics, antifungal, corticosteroids, ear wax softener, analgesics are useful in treating the disordersof external ear. Finally it is concluded that the function of drugs on the external ear is related to a variety of therapeutic approaches aimed atvalleviating symptoms, resolving infections and increasing healing.

Introduction

The external ear, comprising the auricle (pinna) and the ear canal (external auditory meatus), plays a vital role in the process of hearing. While its primary function is to gather sound waves and direct them towards the middle and inner ear, the external ear is also susceptible to various conditions, including infections, inflammations, and other disorders. In such cases, medications are often employed to alleviate symptoms and treat underlying issues. Understanding how drugs interact with the external ear can shed light on their therapeutic mechanisms and potential side effects.

Copper is an important mineral needed fir many physiological activities especially in the human body along with the formation of red blood cells, maintenance of nerve cells and support of the immune system. Whatever it may be, like various substances copper also exhibits toxicity if present in an excessive amounts. Thus article provides an information regarding the intricacies of copper toxicity, exploring it's sources, toxico dynamics, toxico kinetics. Clinical conditions, diagnosis as well as treatment. Copper intoxication can happen through many sources such as environmental as well as occupational. Common sources are contaminated water, dietary sources, occupational exposure as well as medications. Particularly after an ingestion, an absorption of copper takes place essentially in the stomach as well as smart intestine. Now the transportation of copper happens in the blood stream, mostly in bound to ceruloplasmin, a copper _ carrying protein. An elimination of copper occurs primarily through bile secretion into the feces along with a smaller part excreted especially vin Urine. Whatever it may be, in cases of overload, an accumulation of copper takes place in tissues namely the brain, liver and kidneys, resulting in toxicity. Copper toxicity results in the formation of reactive oxygen species ( ROS ), which cause oxidative damage to cellular to cellular structures namely lipids, proteins as well as DNA. Clinical signs and symptoms include gastro intestinal portion, neurological conditions, hepatic abnormalities, hemolytic anemia as well as renal impairment. Lesions of copper toxicity are hepatic lesions, hepatic cellular necrosis, inflammation as well as fibrosis, copper accumulation, biliary changes, neurological lesions, neuronal degeneration, gliosis, de myelination, renal lesions, tubular damage, interstitial inflammation as well as vascular changes

Cadmium, a roxic heavy metal, poses risk in a significant manner especially to human health and the environment. It's wide spread industrial use and presence in various products make exposure a concern particularly for both environmental and occupational settings. Understanding the source, toxico dynamics, toxico kinetics, clinical manifestations, diagnosis as well as treatment of cadmium poisoning is critical in mitigating it's adverse effects especially on human health. Cadmium gains an entry into the environment through natural processes namely volcanic activity as well as weathering of rocks. Whatever it may be, human activities along with mining, smelting, refining of metals and manufacturing of batteries, plastics as well as pigments, are major contributors to cadmium release especially into the environment. Additionally, cadmium is seen in tobacco smoke, resulting in exposure among smokers as well as passive smokers. Once released into the environment, cadmium gains entry into the human body through many routes such as inhalation, ingestion and dermal contact An inhalation of cadmium containing dust or fumes is a prominent route of exposure in an occupational settings especially in industries namely mining, smelting as well as welding. The consumption of contaminated water and food is another general route of exposure especially for the general population. After consumption, cadmium in essential manner accumulates especially in the kidneys as well as liver with a half life of approximately 10 - 30 years in the organs. It also accumulates particularly in bones, where it consists of half life of several decades.

Arsenic is a naturally occurring element that can be observed in various forms in the environment, including soil, water, and air. While arsenic has some industrial applications, it is also notorious for its toxicity to humans. Exposure to arsenic can happen through ingestion, inhalation, or skin contact, with ingestion being the most common route of exposure in cases of poisoning. Here, authors provide information regarding the source of arsenic intoxication, its toxicology kinetics and dynamics, clinical signs and symptoms, lesions, diagnosis, and treatment.

Introduction

In this article, authors provide information regarding source of intoxication also.

Source of Intoxication

Arsenic poisoning can lead to various sources, including:

Contaminated Water

Arsenic contamination of drinking water is a significant concern in many parts of the world, specifically in regions where natural geological formations consists of high levels of arsenic.

Aluminium is a ubiquitous metal and seen in an environment. Source of aluminium happens through dietary souce, food addivitives, packing materials, medications, environmental exposure as well as occupational exposure. Toxico kinetics is related to the interaction of aluminium with biological systems and toxicity also. Toxico dynamics of aluminium is related to the the oxidatve stress, neuro toxicity and bone toxicity. Diagnosis is based on MRI scan and measurement of serum as well as urine. Treatment is dependent on chelation therapy as well as avoidance of exposure. Finally it is concluded that aluminium toxicity leads to health risks in a significant manner along with potential adverse effects on neurological, gastro, intestinal, renal as well as skeletal systems.

Introduction

Aluminium is a ubiquitous metal observed naturally in the environment. It is widely used in various industrial and consumer products including cookware antacids, vaccines, and food additives. While aluminium is normally considered safe in low concentrations and exposure to high levels can lead to toxicity causing a range of adverse effects on human health. In this comprehensive article, authors will provide information regarding its toxicokinetics and toxicodynamics, clinical signs and symptoms, associated lesions, diagnosis and treatment strategies.

Understanding the Toxicity of Macrolide Endectocides

Introduction

Macrolide endectocides are a class of compounds widely used in veterinary medicine to combat internal and external parasites in livestock and companion animals. While these medications have proven to be effective in controlling parasites, there are concerns regarding their potential toxicity and adverse effects on animals and the environment.

Whar are Macrolide Endectocides

Macrolide endectocides belong to a group of compounds derived from naturally occurring macrolide antibiotics, such as avermectins and milbemycins. These compounds have broad-spectrum activity against various parasites, including nematodes, arthropods, and ectoparasites like ticks and mites. Some commonly used macrolide endectocides include ivermectin, doramectin, moxidectin, and eprinomectin.

Toxicity Concerns

While macrolide endectocides are generally safe and effective when used according to label instructions, there are instances where toxicity can occur. Factors influencing toxicity include:

Introduction

Phenothiazines are a class of drugs primarily used to treat psychiatric disorders such as schizophrenia, bipolar disorder, and severe anxiety. While they can be effective in managing these conditions, they also come with potential risks and toxic effects when not used properly. Understanding the toxicity of phenothiazines is essential for healthcare providers and patients alike to ensure safe usage and minimize adverse effects.

Mechanism of Action

Phenothiazines exert their therapeutic effects by blocking dopamine receptors in the brain, thereby altering neurotransmitter activity and reducing symptoms of psychosis. Additionally, they also block other receptors such as histamine and alpha-adrenergic receptors, contributing to their sedative and antiemetic properties.

Types of Phenothiazines

There arevarious phenothiazine derivatives available, including chlorpro mazine, fluphenazine, thioridazine, and promethazine, among others. Each may have slightly different pharmacological properties and side effect profiles, but they share common characteristics regarding toxicity.

Toxic Effects

Extra Pyramidal Symptoms (EPS)

One of the most well-known toxic effects of phenothiazines is the development of extrapyramidal symptoms, which include akathisia, parkinsonism, and tardive dyskinesia. These symptoms can range from mild discomfort to severe motor disturbances and can be particularly problematic with long-term use.

Introduction

Azoles, a class of organic compounds containing a five-membered nitrogen containing ring, have garnered significant attention in various fields, including agriculture, medicine, and industry. These compounds are renowned for their diverse applications, particularly as anti fungal agents in both human and veterinary medicine, as well as in agriculture for crop protection. Whatever it may be, alongside their efficacy, concerns have arisen regarding their potential toxicity and environmental impact.

The Role of Azoles

Azoles play a crucial role in combating fungal infections in humans and animals. They inhibit the synthesis of ergosterol, a vital component of fungal cell membranes, thereby disrupting fungal growth and proliferation. Additionally, azoles are used in agriculture to protect crops from fungal diseases, contributing to higher yields and improved food security.

Toxicological Concerns

While azoles have proven effective in controlling fungal infections, their use raises concerns due to potential adverse effects on human health and the environment. The toxicity of azoles stems from several factors, including their mode of action, chemical properties, and metabolic pathways.

There is no terminal of any kind of research, rather they presence a static snap of the gamut of ecological dynamics and functions.

The following scopes for future research

• To construct modelling on resilience, beyond agriculture like livestock f ishery, forestry.

• To create new Algorithm of prediction in ecological behaviour and dynamics

• Gender and economic factors in resilience study and analysis

• Ecological resilience in different agro climatic zones covering coastal ecosystem, Himalayan ecosystem red lateritic ecosystem etc

• Policy research in ensuring and intriguing ecological resilience as well as social resilience on the common platform of production behaviour

• Importance of ecological resilience in the perspective of climate change and global warming

7.1 Summary

Never ever in the annals of human civilization, the importance of ecological resilience has become so important and imperative as we are perceiving now. Both the need and greed of food economics have driven the resource bases of our ecosystem, to a dilapidated and fragile status. By 2050 the world’s population will touch close to 9.5 billion and we have to scale up our food production 60% more then what it is right now. In India by 2040, we have to cross 450 million tons of our targeted food production to keep rhythm with population growth. By 2035 we are going to cross the population of China. Understanding the cascade of risks for climate change, as well as the vulnerabilities to these risks, is key to frame ways to adapt. Reducing vulnerabilities is key to reducing the net impacts on food security and nutrition and also to reducing long-term effects. Increasing resilience of food security in the face of climate change calls for multiple interventions, from social protection to agricultural practices and risk management..

When population pressure is soaring up, the need to produce more food from less and less available land and water, the issues of ecological resilience are coming up more swashbuckling and humongous. The aftermath of green revolution has contributed to achievement in food security on one hand, the other hand water has been contaminated and depleted, soil has been eroded with a sharp decline of organic carbon. We are losing 15 tons of topsoil per hectare per year and inviting a disruptive agro-ecosystem, the syndrome of which are quite vivid and prominent.

6.1. Data Interpretation and Visualization of West Bengal

Any kind of production management system or decision-making process is being organised in social ecology. No variable cannot behave discrete or free without having any kind of influence as well as impact from the vary operating ecosystem. That is how it is imperative to know and visualize the basic features in terms of its distributive characters. The distribution of features in variables through this networking and interactions ultimately contribute to a holistic visualization of social ecologization under study.

Table 6.1.1 represents the distribution of variables in terms of range i.e. maximum and minimum values, mean, standard deviation and coefficient of variation for Terai Zone, Old Alluvial Zone and New Alluvial Zone of West Bengal.

The average age (x1) of the respondent stands at 49.02. It denotes that so far as responsibility of agriculture and age base livelihood runs at the population so far as the respondent under study is peaks are at the hands of farmers approaching or passing through old ages. On the contrary the absence of young population or withdrawal of young age farmers from agriculture have got a serious implecation near and far. The cv value runs at 25.78 per cent to indicate that the distribution of variable is highly consistant.

The average of functional education (x2) of the respondent stands at 8.20.The variable education charecterization the social ecology speaks that the most of the respondents are having mid level school education.So, this has got spil over effect on the function and operation of other variables i.e. precisely the perception variables.The cv value stands at 43.69 per cent that’s good enough to infer that the distribution of the variable is highly consistent.

5.1 Locale of Research

Based on the All-India assessment report regarding Climate Vulnerability Assessment for Adaptation Planning in India Using a Common Framework which identifies the most vulnerable states and districts in India with respect to current climate risk and the main drivers of vulnerability, the present study has been conducted in two relatively highly vulnerable states of India namely West Bengal (Vulnerability Indices:0.592) and Jharkhand (Vulnerability Indices: 0.674). It specifically comes under Agro-climatic zone III (Lower Gangetic plain region) and Agro-climatic zone VII (Eastern plateau and hilly region) respectively.

On the basis of Vulnerability Indices (Vis) different district of West Bengal classified into three categories i.e. relatively high vulnerability (0.59-0.69), moderate vulnerability (0.49 -0.59), and relatively low vulnerability (0.38- 0.49). Two districts, Malda (VIs:0.690) and Jalpaiguri (VIs:0.627) from highly vulnerable and three district Murshidabad (VIs:0.524), Dakshin Dinajpur (VIs:0.523) and Burdwan (VIs:0.497) from moderate vulnerable category were selected purposively for the present study. For satisfying the purposes three agroclimatic zones of the West Bengal, viz. Terai Zone (Jalpaigudi), Old Alluvial Zone (Murshidabad and Dakshin Dinajpur) and New Alluvial Zone (Burdwan) districts were taken into account for the assessment. Twenty respondents of two Villages from each Block i.e., Alipurduar-I of Jalpaigudi, Hilli and Gangarampur of Dakshin Dinajpur, Old Malda of Murshidabad and Barabani of East Burdwan were purposively picked for the research study.

In any social science research, it is hardly possible to conceptualize and perceive the data and interpret the data more accurately until and unless a clear understanding of the characteristics in the area and attitude or the behavior of people is at commend of the interpreter who intends to unveil an understanding of the implication and behavioral complexes of the individual who live in the area under reference and form a representative part of the larger community. The socio-demographic background of the local people in a rural setting has been critically administered in this section. A research setting is a surrounding in which input and elements of research are contextually imbibed, interactive and mutually contributive to the system performance. Research setting is immensely important in the sense because it is characterizing and influencing the interplays of different factors and components. Besides the investigators must also have the basic knowledge about the socio demographic background of the people so as to have an easy understanding of their knowledge, attitude and behavior.

Research setting refers to the detail information of an area where the study has been conducted. The study area, generally comprises of a particular geographical area viz. a state, district, block, or gram panchayat area selected according to the convenience of the investigator who possess adequate knowledge regarding the location, communication facility etc. of the locality so that he/she can easily approach each and every corner of the area for data collection. Here. in this study the data collection was done mostly over telephone based on the interview schedule due to Covid-19 pandemic situation.

3.1 Conceptual Framework

Aconceptual framework is an analytical tool that is used to get a comprehensive understanding of the study. It embodies the specific direction by which the research will have to be undertaken. It is generally constructed to provide the researcher the element ofattainable and researchable problems and ideas supported by methodology, design, prospective analysis and empirical conclusion. The conceptual framework is often called as the research paradigm.

The subject matter of the present research is Resilience and productivity of agriculture in different social-ecology: the farmers’ perception, status and interpretation. It is expected that the terminologies and concepts used to frame up the topic need to undergo brief explanation for the better understanding of its meaning.

Resilience: Resilience is about anticipating, planning and reducing disaster risk to effectively protect persons, communities and countries, their livelihoods, health, cultural heritage, socio-economic assets and ecosystems. The ideas of ‘bounce back’, ‘spring forward’ and ‘build back better’ are often used in the context of resilience.

Productivity: Productivity is commonly defined as a ratio between the output volume and the volume of inputs. It measures how efficiently production inputs, are being used in an economy to produce a given level of output.

Agriculture: The word agriculture derives from the Latin ager (field) and colo (cultivate) signifying, when combined, the Latin agricultura: field or land tillage. But the word has come to subsume a very wide spectrum of activities that are integral to agriculture and have their own descriptive terms, such as cultivation, domestication, horticulture, arboriculture, and vegeculture, as well as forms of livestock management such as mixed crop-livestock farming, pastoralism, and transhumance. The Oxford English Dictionary (1971) defines agriculture as “The science and art of cultivating the soil, including the allied pursuits of gathering in the crops and rearing livestock; tillage, husbandry, farming.”

This chapter is an extensive survey of all past studies relevant to the field of investigation which gives a knowledge of what others have found out in the related f ield of study. In any scientific investigation or research a comprehensive presentation of review of literature is a statutory requirement. Its main functions are to develop a better understanding of the problem to be investigated, delineate a new area of study and avoid unnecessary duplication, decide on the tools and techniques to be adopted including developing some new ones, also to relate the present study with the previous ones by finding out the areas of agreement and disagreement.

The review of literature of the present study is based on the major concepts related to the f ield of investigation. As direct references on all the item are not in abundance, certain specific references along with some indirect references have been incorporated in this chapter for the purpose of meaningful use.

Review of literature has been organized with a view to provide hard evidences as to clinically justify the reasons, logic, and contemplation leading to heuristically infer the different echelons of resilience issues.This is also catered to isolate the apparently less attempted genre of resilience study and analysis. Climate change, uncertainty, decline of soil organic carbon, fatigued genes on land, loss of biodiversity and fragile ecology of society have only been some of myriad of factors causing the loss of resilience, from ecology and from society as well.

1.1. Background

Once this beautiful planet conceived human civilization with the glory of motherhood, the birds kept on twittering in an ecstasy of joy and freedom; the ripples of pristine rivers, blue and giggling, offered shelter to silvery fishes and pink Amazon Lilies! But human greed has defeated its own needs. So, what had been a means to survive now has turned into tools to triumph, rather, a weapon to destroy her own creation. By this time, we have transformed fertile lands into a barren Arizona, deep forests into a nasty slums and blue rivers into a filthy stream, called Yamuna.

The advent of the Anthropocene, implies that an unprecedented era of human impact on the natural environment, is now so large that the Earth has entered a new geological epoch; it is leaving the Holocene, the environment within which human societies themselves have developed (Gornish and dos Santos, 2016; Reynolds, 2021). Climate change is a prominent sign of human- driven changes to the global environment. There is every reason to be alarmed about anthropogenic climate change, pollution, and biodiversity loss, all of which have been accelerating in recent decades and do pose “existential crisis” (Díaz et al., 2020; Abhilash et al., 2021). Sixth Assessment Report of Intergovernmental Panel on Climate Change (IPCC) released on February 28, 2022, paints a troubling picture as limiting global warming to 1.5oC (a global target in the Paris Climate Agreement) is not safe for all. If warming exceeds 1.5oC, even temporarily, much more severe, often irreversible effects of climate change will occur, land and ocean temperature change, sea level rise and ocean acidification, loss of Arctic Sea ice and ice sheets, stronger storms, longer heatwaves and droughts, abrupt precipitation mainly intensity and seasonal distribution, thawing permafrost and more (IPCC, 2013). On the basis of the Sixth Assessment UN Secretary-General António Guterres said “The alarm bells are deafening, and the evidence is irrefutable: greenhouse gas emissions from fossil fuel burning and deforestation are choking our planet and putting billions of people at immediate risk.” According to the United Nations (2015), there are still 836 million people in the world living in extreme poverty (less than USD,1.25/ day). And according to the International Fund for Agricultural Development (IFAD), at least 70% of the very poor live in rural areas, most of them depending partly or completely on agriculture for their livelihoods. It is estimated that 500 million smallholder farms in the developing world are supporting almost 2 billion people, and in Asia and sub-Saharan Africa these small farms produce about 80 percent of the food consumed (IFAD, 2011). The rural poor often depend partly on forests for their livelihoods (World Bank, 2002). It is estimated that between 660 and 820 million people (workers and their families) depend totally or partly on fisheries, aquaculture and related industries as a source of income and support (HLPE, 2014).

Veterinary ultrasonographer has to write report of ultrasonography in different animals. The proper ultrasound report writing is the moral duty of the vet ultrasonographer. The accuracy of diagnosis must be ensured. The poor resolution/quality of machine may lead to erroneous diagnosis. Therefore, veterinarian must ensure good quality of machine and proper diagnosis. The report might be different in different animals e.g. report writing about uterus will be different in bitch, cows, buffaloes, camel, horse, goat and sheep. However following might be guidelines in writing ultrasound report of uterus. fetus, endometrium

Uterus: Position of uterus, contents in the uterus, shape and size in initial pregnancy and infections, appearance of myometrium, thickening of uterine layers, presence or absence of fluied/pus/masses, left and right horns and their contents. Observations on the images of uterine fluid and its echoicity, membrane appearance in pregnant animals. Special attentions should be paid to endometrium.

Endometrium: Appearance and thickness; any growth on the surface of endometrium. Images of retained portion of placenta can be ascribed in the cases of retained placenta.

Ovary: Ovaries are scanned to assess cyclic structures, size, shape, its Position/ location and internal echo pattern of follicles. This echo pattern will indicate whether follicle is normal, cystic or with any other abnormalities of follclular f luid. Ultrasound reports also include shape and size of corpus luteum. In cows and buffaloes, presence of cavity, looseness/compactness of luteal cells are also mentioned.

Ultrasonography of the GI tract presents problems and challenges for the sonographer. Sonography of the GI viscera was once throught to be impossible because of artifacts created by gas within the lumen but with the technical advancement in the ultrasonography now the problem has been solved. If the animal is kept of feed and adlib water is given to the dog with I/v fluid than ultrasonography becomes helpful in the diagnosis of gastrointestinal tract disorders. Other conditions such as esophageal carcinomas can also been diagnosed by ultrasonographic endoscopy. in human beings.

The normal ultrasonography of the liver, kidney, stomach & spleen in dogs has been standardized and its use in evaluation of pathological lesions has been reported but ultrasonographic evaluation of oesophagus & intestine, both in normal animals and diseased conditions is yet to be established. Therefore normal ultrasonography of the oesophagus & small intestine in dogs and the importance of diagnostic capability of ultrasonography in diagnosis of pathology of these organs have been compiled.

Mammary glands are important part of body which synthesize and store the milk in between milkings or suckling by the calf. Therefore it attains prime importance in any dairy animal. However, it has received little attention in buffaloes till now. Buffalo presents several unique characteristics in terms of milk production, length and shape of teats and arrangement of duct system in the mammary glands. Development of udder may be related to milk production and the knowledge of changes in its structure in relation to different physiological stages may also have practical implication.

Transducer Selection and Preparation of Scanning

Preparation of the site to be imaged will enhance the quality of the image. Therefore, any loose hair and dirt must be clean off. Ultrasound gel is applied to the area of interest. The gel acts as a coupling agent to prevent air from trapping between the transducer and skin. A 7.5 MHz transducer is ideal for imaging the teats. A 5.0 MHz transducer provides an adequate image. The mammary gland should be scanned with a 5.0-7.5 MHz transducer to give adequate penetration. All structures should be scanned in cross section and longitudinal planes.

This paper primarily considers the general principles of ultrasound and instrumentation and its application in camel reproduction.

Ultrasound waves are mechanical pressure waves similar to audible sound waves and must have a medium in which to audible sound waves and must have a medium in which to propagate. The “frequency” (f) is the number of high- or low- pressure regions crossing each area of tissue each second. The frequencies of the sound waves used in medical ultrasound are much higher than the human audible range (20 to 16000 Hz); hence the waves are called ultrasound. Ultrasound instrumentation

The gray scale imaging equipment uses only time delay and echo amplitude information in creating the image. Most equipment contains the same basic building- block circuits; transmitter, receiver, display and scan converter.

Transmitter

The first circuit building block, the transmitter circuit produces either a high- amplitude, short- duration voltage shock pulse or a driving voltage waveform. Then an output control attenuates the amplitude of the shock pulse or the driving voltage wave form before it is applied to the transducer.

Corpus luteum, one of the biological clock of the estrous cycle and pregnancy, is known foremost for its production of progesterone that blocks the pituitary release of gonadotrophins and prepare the uterus for pregnancy. The ovarian follicle wall consists of granulosa cell and theca cells vascularised and luteinize after ovulation to from a corpus luteum. Corpus luteum is temporary steroid- producing gland undergoes marked structural and functional changes far a short time span during its development functional life and regression. During estrous cycle, the corpus luteum undergoes an initial period of growth (metestrus), followed by a period of maximal function (diestus) and finally by a period of regression (proestrus) preceding the next ovulation (Robert, 1986). The most prominent cell in the CL is the large luteal steroidogenic cell.

Ultrasound imaging has been used to detect and measure the corpus luteum throughout the estrous cycle and corpus luteum has been characterized during pregnancy and non pregnancy. Changes in corpus luteum diameter as detected by ultrasonography are highly correlated with progesterone production. A high correlation has been found in the ultrasonographic detection of bovine corpora lutea (Lean et al., 1992) and between luteal tissue area measured ultrasonographically and plasma progesterone concentration (Bergfelt et al., 1989).

Real time ultrasonography provides means to directly image the reproductive tract. An extensive amount of information can be gained from even a single examination, including determination of ovarian (luteal or follicle detection) and uterine status, detection and monitoring of ovarian and uterine pathology, monitoring the post-partum mare, detection of twins, prediction of ovulation, fetal growth and development and determination of sex of fetus. When first introduced the use of ultrasonography was primarily limited to early pregnancy diagnosis only, with advancement of knowledge and interpretive skills, the diagnostic ultrasonography has a much more application in reproduction. Some of the applications are discussed in this lecture.

Procedure

The procedure and precautions for intrarectal ultrasonographic examination are similar to those for rectal palpation, and no additional restraint is required. The transducer should be protected by the examiner’s hand to prevent trauma to the rectal wall, and the transducer should be well-lubricated. Care should be taken to avoid fecal material attaching to the transducer. After evacuating fecal material from the rectum, the probe is introduced and moved across the reproductive tract in the following pattern: uterine body, right uterine horn, right ovary, right uterine horn, uterine body, left uterine horn, left ovary, left uterine horn, uterine body then cervix. Good contact must exist between the transducer and rectal wall. Air in the rectum or a gas or fluid-filled loop of bowel will result in a distorted image. To minimize scanning errors, principally those of omission, it is recommended to conduct the same scanning procedure during each examination.

Ultrasonography in bull is helpful in diagnosis of problems of testis and accessory glands. This can also be used in assessment of development of these organs. Such developmental assessment, when combined with endocrine data, is useful to know age of puberty, sexual development and dimension of these organs. The ultrasonographic images and data are available for testis, bulbourethral gland, prostate and seminal vesicles in the bull. Ultrasonography of testis of bull can be useful to assess developmental changes, testicular dimensions and pathological conditions of the testis viz. tumors, lesions, abscess etc. Epididymis can also be examined for change in lumen, inflammation, granuloma etc. The accessory sex glands can be better viewed in ultrasonographic scanning to assess their dimensions, normal developmental changes and alterations in their morphology. Before, ultrasonography is undertaken, one should thoroughly revise the knowledge of sectional anatomy and location of these organs.

Testis

Location: The testis, in the bull, is located in the scrotum. In abnormal conditions, the testis might be retained in the inguinal canal or abdomen. Before, ultrasonography, location of the testis should be assured. For this, the testis should be palpated to assess their pliability and rule out adhesions with the scrotal wall. In normal condition, the testis should be freely movable in the scrotum, soft of palpation and both the testis should touch the bottom end of scrotum.

Ultrasonographic images of stomach are required to diagnose ingestion of any abanormal materials by dog. When content of stomach are dry or there is gas in stomach then, it is difficult to get proper image of stomach. Offering water to animal or oral administration of water with the help of syring is also helpful in getting the images of content of stomach. Some time, while doing ultrasonography, movement of contents of stomach can be visualized. Stomach can be images between liver and spleen. The size depends upon contents or intake of food by dog. For proper image of gastrointestinal tract including stomach, there should not be gas in GIT. One should remember that ultrasound beam does not travel in gas. The best medium of ultrasound beam is liquid.

Scanning Technique

If possible, fasting for 12 hours is good to diminish the amount of gastrointestinal gas. However, non-fasted dogs, when offered water may show adequate image quality. The water may be administered in quantity of 15 ml per kg of bodyweight. Removing the stomach gas using an orogastric tube has been recommended tto enhance the visualization of lesions of the stomach, however, this is not in practice in India.

The Liver

The liver is a large and an irregular organ of the body, which extends from the sixth to the eleventh intercostal space. It extends much more caudally on the right side as compared to the left side. Its most caudal part is the caudate lobe whose small portion is visualized encircling the right kidney dorsally at the eleventh intercostal space. The liver of a dog is divided into various lobes namely the left lateral, left medial, quadrate, right medial, right lateral and the caudate lobes which are not identifiable ultrasonographically, except when f luid is present in the abdomen. Otherwise, the position of the different liver lobes can only be approximated. The gallbladder is located to the right of the midline between the quadrate and the right medial lobes of the liver.

Proper preparation of the animal is important to obtain images of abdominal orgnas including that of liver. It is better to keep animal off fed for at east six hours prior to ultrasound scanning. This will avoid interference of gases and solid contents of the gastrointestinal tract. If stomach is taken as landmark of scanning then enough fluid should be given prior to scanning to get good images of stomach. The liver can be easily visualized on one side of stomach. One should be liberal in applying good amount of gel over transducer and body for better image of liver.

Introduction Ultrasonography is well-established technique in human sciences, particularly for diagnosis of pre-natal defects of fetus. In veterinary sciences, this technique is emerging an important tool. This tool is being employed in the small animal practices, particularly bitches for diagnosis of various reproductive disorders and estimation of gestational status. In the past, veterinarians had limited assistance for assessing reproductive status of this animal. Pregnancy diagnosis in a bitch was possible either with the use of X-rays or transabdominal palpation, but that too at an advanced stage of gestation. These methods could provide information either about presence of pups in vitro, or their number, but assessment of fetal viability was not accurately predicted with these traditional methods. With the use of X-rays, there was always risk of harmful effects on the health of mother and fetus. As far as reproductive disorders are concerned, most of them remained either undiagnosed due to the lack of proper access to the internal organs or were diagnosed with a guess. Diagnostic ultrasound is a valuable alternative imaging system that can provide more accurate information about the canine pregnancy and reproductive disorders in comparison to traditional methods.

Ultrasonography in sheep is becoming popular in the field of reproduction and replacing the other old-age techniques, viz. abdominal ballotment, recto- abdominal probes, X-rays and radioimmuno-assays. The reasons are numerous such as unsatisfactory results, hazardous effects to animal, operator or environment, high cost, long delay in diagnostic results and requirement of sophisticated labs. The ultrasound system has low operating cost and does not need special labs as it can be done at farm itself. Ultrasonography is considered safe to sheep and the operator and provides quick and reliable results. The popular opinion about ultrasonography is that it is noninvasive imaging system and is rapid, safe and practical means of diagnosis of pregnancy in sheep. The B-mode real time ultrasonographic system particularly portable system is popular among sheep breeders. In sheep, B-mode real time ultrasonography was used in 1971, using 5 MHz frequency transducer. A transducer of switchable frequency between 7.5 to 5.0 is more popular in sheep practice.

Transrectal Procedure in Sheep

The procedure is similar to goat, but sheep remain quite during transrectal ultrasonography than goat. Following are some of the steps to obtain good quality image:

Ultrasonography in goat is becoming popular in the field of reproduction and replacing the other old-age techniques, viz. abdominal ballotment, recto- abdominal probes, X-rays and radioimmuno-assays. The reasons are numerous such as unsatisfactory results, hazardous effects to animal, operator or environment, high cost, long delay in diagnostic results and requirement of sophisticated labs. The ultrasound system has low operating cost and does not need special labs as it can be done at farm itself. Ultrasonography is considered safe to the animal and the operator and provides quick and reliable results. The popular opinion about ultrasonography is that it is noninvasive imaging system and is rapid, safe and practical means of diagnosis of pregnancy and reproductive disorders in sheep and goat. The B-mode real time ultrasonographic system particularly portable system is popular among researcher and practitioners. In goat, B-mode real time ultrasonography was used in 1971, using 5 MHz frequency transducer. A transducer of switchable frequency from 7.5 to 5.0 is more popular in small ruminant research and practice.

The ultrasonography is based on echo-principal i.e. reflection of sound waves. The ultrasound waves, commonly termed as ultrasound beam is generated from the pizzo-electric crystals, under electric stimulation. The sound beam travels and strike to the organs/tissues on its way and get either reflected or reabsorbed by these structures. The reflected beam is received back by the transducer, which are processed and displayed on the monitor of ultrasound scanner. The display of signal is in form of shades of grey scale i.e. from bright to black picture. If these sound beams strike to organ such as bone, then major part of the beam is reflected and it appears bright (white) on screen. If the sound waves strike to fluid, these get absorbed and are not reflected back, therefore, the image of fluid appears as black on the screen. Other organs appear in between black and bright white. In fact, the reflected sound beam is not proportionate to frequency and velocity of emitted ultrasound beam. The strength of ultrasound beam (grey scale) or brightness of the image depends upon the acoustic impedance of organ or tissue. In simple term acoustic impedance is the capacity of tissue or organ to reflect or absorb these sound beams that again depends upon density or composition of these structures. The reflection of sound beams also depends upon, the angle at which the ultrasound beam strikes the structure on its way. The distance of the organ from the transducer also affects image brightness.

Diagnostic techniques are routinely used for diagnosing different surgical conditions. The most frequently used imaging techniques in veterinary practice are radiography and ultrasonography. Radiography and ultrasonography are not suitable for each organ in large animals. In small animals most organs are suited to ultrasound images except bones and gascontaining structures such as lungs etc. Bone and gas reflect ultrasound and do not allow the sound waves to form image therefore radiography gives better diagnostic images for these organs. The liver, spleen, kidneys, prostate, gravid uterus and fluid containing organs like urinary bladder, gall bladder and major blood vessels can be better imaged with ultrasound. In buffaloes in surgical section important clinical conditions relate to urinary and digestive systems, mammary gland and perineal area.

1. Urinary System: In bovine, the cases of obstructive urolithiasis are commonly observed. These types of cases are more common in bullocks and in buffalo calves. In these cases radiography is not so useful. Sonography can be tried to take the image of ruptured urethra in bullock and can be compared with radiography. Due to ruptured urethra there will be accumulation of urine in the s/c tissue of prepuce. Due to fluid density the image will be radioopaque and will appear white and no detail of the organ can be seen. The ultrasonogram (USG) will show images of glans penis and its coverings. Different diameters of urethra and a point of rupture just behind to glans penis can be make out. Due to obstruction, The B.calves either will pass drop wise urine or there will be complete stoppage of the urine. These cases can also be easily diagnosed by ultrasonography.

In Doppler imaging, the reflections are from circulatory vessels, while in 2D ultrasonic image the reflection is from a tissue(s) or organ(s) In 2D ultrasound strength of reflection in B-mode depends upon density of the organ.On other hand the reflection in Doppler imaging depends upon direction of the circulation. If circulaltion is coming towards transducer, the signal will be strong, while it looses its strength while flowing away from the transducer. As shown in the diagram, the position of transducer on curved surface decides the strength or amplitude of signal. If it is positioned on curve end from where circulation is coming, the signal will be of higher amplitude, but when position is changed to other end of curved surface, where circulation is going down the amplitude of signal will decrease accordingly and is shown in reverse direction.

Principle of Doppler

Movement of blood in vessels is detected, which is depicted by color in some ultrasound machines. The flow coming towards transducer is shown above baseline, while flow going down i.e. away from transducer is shown below baseline. If position of transducer is changed from where the arterial flow is going away from transducer then, the arterial flow will be shown below baseline and venous flow that is coming towards transducer will be shown above baseline. Some machine shows red and blue color of the flow. The red color shows flow coming towards and blue color shows flow going away from transcer. A beginner ultrasonographer may think that the red color is from artery and blue is from vein. This is not true, this shows direction of flow. Since ultrasonic image of flow in Doppler is formed from series of pulses returning from vessels, the magnitude of pulse changes with variation in flow. A stationary flow will not show any height of magnitude.

Ultrasonography has an importance place in human practice and veterinary f ield especially in canine practice. Since many years two dimensional ultrasound has been used for scanning purposes both in human and animal practice. But the scenario has changed in human practice with development of three dimensional technology and later four dimensional ultrasonography has developed which shows movements of the objects being scanned especially in the case of fetuses. Nowadays it has been common to use these advanced technology in the human practices. Yet these technologies are in the childhood stage in the veterinary field. High cost of 3D/4D ultrasound equipment, operational difficulties in non sedated animals & lack of specific knowledge about the operation of volume scanning hinder the use of this technology in the veterinary field. Better understanding of the different application of these technologies can be made to use in research areas for the better understanding of the abnormalities, post therapeutics as well in the diagnosis of the diseases

Principle of Three Dimensional and Four Dimensional Ultrasonography

Sound waves of frequency higher than human audible frequency usually ranges from 2-13 MHz are used in ultrasonography. Depending upon on depth of tissue from the scanning surfaces frequency is selected. Three dimensional ultrasonography gives three dimensional images of the volume being scanned .it works on the same principle of 2D ultrasonography but ultrasonographic beams are sent different angles to get three dimensional image of object being scanned. Volume information is obtained either directly using 2D array probe or by reconstructing three dimensional volume from a series of continuous or non- continuous 2D images by using software (Fenster and Downey, 2000). An advancement of 3D technology is 4D ultrasound. Four dimensional ultrasound adds the fourth dimension as time and gives a dynamic real-time 3Dultrasonography of the concerned organ with a continuous volume image acquisitioned rendering process. (Khurana and Dahiya, 2004).

Congenital anomaly of newborn buffalo calves is quite common in new born calves. Most common encountered are atresia ani, rectovaginal fistula etc. Sometimes, developmental defects interfere with physiological processes viz. in urination and defecation. Therefore, such cases, if not corrected well in time can cause death of the patient. In the present report, few newborn calves were brought to the veterinary clinic with the following abnormalities:

Agenesis of Urogenital System Without Anus

Five newborn buffalo calves were brought to the veterinary clinic with the complain of not passing urine and feces. There was no visible external genitalia and anal opening. At the site of vulva, there was only an impression of vulvar lips and below it, a cord like structure was hanging.

There was an impression of round structure below the skin; in some cases it was outside giving appearance of scrotum which was located in between the two thighs. On ventral side of abdomen, preputial sheath was not present, therefore, it was very difficult to decide about the sex of the calves and site of external preputial-orifice. There was an indication of both sexes, but organs were not developed, therefore it was difficult to decide about the sex of the calves. In some cases, impression of the anal sphincter was visible but there was no visible external anal opening. There was slight deviation of the tail from its base also. Intake of milk was normal. Abdomen was distended bilaterally. Respiratory rate was higher than the normal with slight discomfort .Heart rate was elevated. Blood picture revealed increased total blood cell count with neutrophilia. Radiography of the abdomen showed distended urinary bladder at the floor of pelvis indicating formation of the urogenital system without development of the external opening. Barium meal study revealed marking of gastrointestinal tract and stagnation of the barium up to the rectum.

Ultrasound is mainly used for reproductive purposes in bovine, now days it is also used for other applications. Ultrasound is an excellent tool for diagnostic imaging in large animals because it is portable, easy to use in the field, produces results at the time of the examination. It is totally non-invasive. Palpation of a gland can only detect certain levels of abnormality. Ultrasound can give an actual perception of mammary health, a picture of the inside anatomy and pathology. Tumors, scarring, stones, trauma, hematoma formation and level of damage due to mastitis can all be evaluated by this technique. Other diagnostic tools are still primary diagnostic tools, ultrasonogram gives yet another dimension to the hard to diagnose case. It will also allow a better estimate of treatment prognosis to aid in deciding whether to cull or treat a dairy animal.

Congenital anomalies at initial stages of the mammary gland development during gestation may result into presence of supernumerary teats or less number of external teats, in place of usual presence of four teats in buffaloes. Hence, cases with two / three / five or six teats are often encountered in the f ield.