eBook Chapters

HAEMOPROTEUS

Host

Infection with Haemoproteus species is host specific.

H. meleagridis – domestic poultry and pet birds

H. olumbae in pigeons

H. nettionis in waterfowl.

With few species-specific differences in analyte ranges, clinical chemistry instruments originally developed for human and veterinary use are readily adapted for use in fish specimens. However, the development of hematological methods for fish continues to face challenges similar to those encountered during the early applications of complete blood count (CBC) techniques in birds and reptiles. Like their non-mammalian terrestrial counterparts, fish possess nucleated erythrocytes, and several leukocytes—such as thrombocytes, monocytes, lymphocytes, and basophils—exhibit similar morphology when stained with Romanowsky-type stains.
Blood collection and hematological testing are essential techniques in fish pathogen detection and overall health monitoring within India’s aquaculture systems. These approaches help assess physiological responses to infectious agents, environmental stress, and farming practices. Given the wide variety of cultured species—from carps in West Bengal and Andhra Pradesh to catfish in Tamil Nadu and ornamental fish in Kerala—blood-based diagnostics serve as a non-lethal, rapid, and informative method for health evaluation.
Blood is commonly drawn from the caudal vein or heart using sterile syringes, with or without anticoagulants such as EDTA, depending on the intended analysis. In field conditions, particularly in rural hatcheries and pond systems, affordable options like glass capillaries or insulin syringes are frequently employed. For small-sized species such as Danio or ornamental fish, heparinized capillary tubes are used to reduce clotting during blood collection. Fish are typically anaesthetized using clove oil or MS-222—both widely accessible in Indian laboratories—to minimize stress during handling.

Background
Blood collection from cows and other animals is a routine procedure often performed for diagnostic, research, or health-monitoring purposes. Diagnostic testing, such as hematology, biochemistry, hormone assay, infectious disease testing, and animal pregnancy diagnosis, can be done using blood. Blood samples are required for genetic testing (DNA fingerprinting or parentage determination), pharmacokinetic studies, and immunological studies. Another use of blood collection is monitoring animal health.
It is essential to consider that proper techniques of blood sample collection should be followed. Avoid introducing infection to the animal during blood collection. Remember the animal welfare to minimize distress and discomfort to the animal during blood collection.
Materials Required
• Sterile syringe or vacutainer (usually 16-18 G needle)
• Blood collection tubes (BD Science K2 EDTA Vacutainer(R) PLUS 6 mL) for whole blood, serum tubes for serum collection, or other specific tubes depending on the test (Figure 1).
• Antiseptic solution (e.g., alcohol or iodine)
• Restraint (head gate or halter)
• Gloves

Amounts up to 5 ml of blood can be safely drawn from the auricular marginal vein, using a 23- to 25-gauge needle (or) a 23-gauge butterfly attached to a syringe or tube. The jugular vein should be avoided for blood collection if the rabbit suffers from a respiratory problem or shows respiratory distress. It may become cyanotic during the procedure. Alternate sites for blood collection are the cephalic and the lateral saphenous veins. (Their locations are the same as in dogs and cats. These veins are fragile, and a hematoma is easily formed).

Accurately in the clinical diagnosis of a disease in experimental animals frequently depends on the result of laboratory examination of blood specimen from the diseased animals. A prerequisite for good experiment result is the proper collection and handling of the blood samples. If properly collected samples can not be sent to the laboratory quickly. It must be preserved or stored in such a manner that the necessary tests may be conducted. The technician/researchers

Going to collect the blood from a laboratory animal should be aware of the purpose of blood collection, the sites of blood collection, the technique of blood collection, the Use of various anti coagulants, the techniques of blood examination.

Blood Examination of Animal may be Necessary

The glucose is utilized in the body for immediate energy requirements. The utilization of glucose depends on the integrated activity of digestive system metabolism and endocrine system of the body. Glucose tolerance is referred as the capacity of animal to regulate elevated blood glucose on ingestion or infusion, gradually. The level of glucose that can be tolerated by an animal without producing glucosurea is indicative of tolerance. Glucose load challenges the pancreas to release insulin which in turn regulates blood glucose level. If the insulin release is adequate and target cells have healthy receptors, elevated blood glucose level return to base value within 2 hours and glucose does not appear in urine. An increased glucose tolerance means limited rise and rapid fall in blood glucose in short span than the normal rate of utilization.

Blood Cholesterol

(Colorimetric Method of Lieberman, 1885: Burchard 1889 Modified by Watson, 1960).

Introduction

According to Liebermann-Burchard reaction, treatment of chloesterol with acetic anhydride and concentrated sulphuric acid in a water-free environment, polymerized unsaturated hydrocarbon with an intense blue-green colour are produced. The values for cholesterol esters may be calculated by deducting free cholesterol from total cholesterol.

We must know our family, its members and their relationships. Let us first look at basic relationships which often figure in the questions. If you are conversant with the given items in the table below, half of your battle is already won. It is not required to mug-up these names, but instead make a clear picture in your mind as you go through each one of them. This will go a long way in retaining the details of these given shortcut tricks for solving Blood relations problems for logical and analytical reasoning. Read the following thoroughly before moving on

Blood transfusion is one of the crucial life-saving procedures in veterinary medicine undertaken to increase red cell mass, volume replacement, replenishing coagulation factors, and protein replacement. The concept of blood transfusion was first introduced by Francis Potter in 1639. However, it was only in 1666 that Richard Lower reported the first successful direct transfusion from one dog to another. Blood is a biological drug and is not a definitive treatment, but it provides support until the disease is diagnosed or treatment is initiated. Indications Blood transfusion is indicated for the management of the following conditions: •     Anemia due to various diseases/disorders, like infection or acute/ chronic hemorrhage •     Bleeding disorders such as thrombocytopenia or coagulopathies •     Rodenticide toxicities

22.1 Introduction

The blood vascular system of fishes is modified to suit the aquatic mode of life. Fish contains the simplest single-circuit closed blood vascular system.

The structure of the heart in fish varies little. In fish, all of the chambers of the heart are essentially the same. The size and shape of the component chambers may differ. A large heart is found in extremely sluggish fishes with low oxygen requirements. The number and arrangement of valves in the conus arteriosus are the most distinguishing features in various fishes.

Blood is a first by-product of animal slaughter house. It accounts about 5-7 percent of animals live weight. It is highly nutritious material particularly rich in protein (17-18%) and essential nutrients. Blood is a rich source of amino acids like leucine, lysine, valine, phenylalanine, histidine etc. It is also rich in haem iron which is biologically most available form of iron. Protein efficiency ratio of plasma is 2.5 while 1.0 for globin. It contains about 80 percent water and responsible for effluent problems. It is a great source of pollution because BOD₅ of whole blood is 250000 mg/litre. It should be collected and processed as soon as possible because whole blood clots easily and is capable to clogs the drainage system, grease traps and septic tank of meat plant or abattoir.

Whenever we get a cut, a red fluid comes out of the wound. This red fluid is called blood. Blood is very important for the body as is evident from the fact that the loss of blood from the body makes it weak and may even cause death. Physiologically the blood is a red tissue composed of cells suspended in a pale yellow fluid, plasma. Unicellular organisms that live in immediate contact with the external environment obtain nutrients directly from that environment and eliminate unused or unwanted materials. Blood and lymph are important connecting fluids among the diverse anatomical structures of the mammalian organisms. Thus blood is the liquid vehicle by which the major organic nutrients are transported from the intestine where they are absorbed, to the liver where they are processed and thence to other organs. Similarly blood is the liquid vehicle for the transport of waste materials from various organs to the kidney and gastrointestinal tract from where they are eliminated. Thus the blood is the major transport carrier of nutrients, metabolites, oxygen, hormones, waste products etc. inside the body. Blood is an important medium to maintain homeostasis of various electrolytes in the body. Besides blood provides the army against any external attack on the body by harmful microorganisms. This army is in the form of cells, called white blood or corpuscles or leucocytes.

In a normal adult human being, about 5 to 6 litre of blood is present which is about 8 percent of the body weight. In domestic animals generally 5 to 8 per cent of the body weight is blood (say 30-40 litre in a cow). The blood is composed of two parts, the cells and the fluid part or plasma. The cellular constituents of blood include erythrocytes or red blood corpuscles, leucocytes or white blood corpuscles and thrombocytes or platelets. The cellular constituents of blood varies with the species as well as with the age and physiological stage of the animal.

The extracellular constituents or plasma is composed of water, electrolytes, proteins, lipids, glucose, enzymes and hormones etc. The blood undergoes coagulation on exposure to air leaving clear watery fluid, called serum. The serum is nothing but plasma minus fibrinogen and other clotting factors. The serum is a clear yellowish fluid. The yellow colour is due to the presence of small quantities of bilirubin, a bile pigment and of carotenoids. The blood serum of cattle is yellow due to presence of more of carotenoids whereas that of buffalo is white due to the absence of carotenoids. The blood clot consists of a network of fibrous strands of fibrin in which are enmeshed the cellular elements of the blood. Blood clotting is a protective mechanism against excessive loss of blood from the body. The lymph also clots, although, somewhat more slowly than blood.

Blood is the fluid pumped by the heart that circulates throughout the body via the arteries, veins, and capillaries . Blood is a body fluid in animals and human beings that delivers necessary substances such as nutrients and oxygen to the cells and transports metabolic waste products away from those same cells. Hematology is the science or study of blood, blood-forming organs and blood diseases.

Blotting is the procedure through which nucleic acids or proteins are immobilized onto a solid support generally nylon or nitrocellulose membranes. Blotting of nucleic acid is the central technique for labeling and hybridization on membranes have formed the basis for a range of experimental techniques involving understanding of gene expression, organization, etc. More recently the identification of abnormal genes in genomic DNA has become increasingly important in clinical research and genetic counseling. We can identify inherited disease, infectious agent present in the sample by using blotting technique. It is also used to mapping restriction sites in single copy gene.

Southern Blotting
Introduction

Southern hybridization was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s. To oversimplify, DNA molecules are transferred from an agarose gel onto a membrane. Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. It is also called Southern blotting since the procedure for transfer of DNA from the gel to the nitrocellulose filter resembles blotting. In Southern hybridization, a sample of DNA containing fragments of different sizes is subjected to electrophoresis using either polyacrylamide or agarose gel.

1. Introduction

With the advancement of science and technology, new technologies have been developed to advance molecular work to a great extent. Thousands of DNA molecules can be simultaneously sequenced at one time using next-generation sequencing (NGS). Next-generation sequencing (NGS) technologies act as revolutionary tools for various implementations and can produce hundreds of gigabases of sequence data within a single experiment. A wide variety of modern sequencing technologies go by the name of "high-throughput sequencing." In recent years, this technology has advanced as a de facto tool for the study of genomics and epigenomics. Nucleotides can be located using this technique anywhere in the genome or in groups of specific DNA or RNA regions. An NGS plot is a useful tool for bridging the gap between enormous datasets and genomic information in this era of massive sequencing data. Resequencing of genomic DNA will undoubtedly be the primary application of NGS in clinical settings. Whole genome sequencing (WGS) offers an intricate representation of single nucleotide variations (SNV), insertions and deletions (indels), complex structural alterations, and changes in copy number, all of which are attainable through a single analysis. Extensive genetic information can be obtained and used after complete genome sequencing, which reveals a large amount of genetic data. However, a significant portion contains either novel information or data whose clinical significance has not yet been established. WGS is the most comprehensive genetic analysis of an individual or cancer genome.

BGA belong to a class of prokaryotic photosynthetic microorganisms also known, as cyanobacteria are capable of fixing atmospheric nitrogen aerobically. Most abundantly found in tropical zone, never form fibrous growth and are enveloped by a sheath. The trophic independence of algae for C and N makes them responsible for a natural fertility buildup of rice field soils. The nitrogen fixing ability of BGA was first recognized by Frank and Prantil in 1889. The occurrence of the BGA in the rice field was reported by FE Fritsch in 1907.

Rice is grown in about 40 million hectares of land in India and nitrogen requirement for the crop is 60 lakh tonnes based on the present levels of consumption. Rice production has to be sustained and increased to provide staple diet to our increasing population. Blue green algae is a potentional biofertilizer in rice because of their capacity to meet both carbon and nitrogen requirement naturally from the air. They are microscopic algae which are collectively called phytoplankton produce basic organic matter forming the first link in food chain and alongwith replenish the oxygen content of atmosphere. Algae contain proteins, Vitamins, iodine, bromine and substances of antibiotic and stimulatory nature. The soil algae are divided into chlorophyta (green algae) cynophyta (blue green) bacillariophyta (diatoms) and xanthophyta (yellow-greens).

De (1939) suggested that fertility of rice fields is maintained due to the growth of blue-green algae. Different types of blue-green algae have been reported to colonize the rice fields. The cells such as heterocysts in Anabaena, Nostoc, Tolypothrix etc. which have capacity to fix atmospheric nitrogen under aerobic condition and unicellular and non- hetercoystous forms also fix atmospheric nitrogen under microaerophilic conditions.

Synonyms : Catarrhal fever, Sore mouth, Epizootic catarrh, Sore muzzle, Pseudo foot and mouth disease, Malarial catarrhal fever.

It is an infectious, noncontagious arthropod borne viral disease of domestic and wild ruminants namely sheep, goat , cattle, camels, llamas, deer and antelopes. This is predominantly a disease of sheep but occasionally cattle and  goats are subclinically affected. The disease is characterized by high fever, catarrhal stomatitis, rhinitis, enteritis and lameness due to inflammation of the coronary bands and sensitive laminae of the feet. There are at least  25 serotypes of bluetongue virus worldwide within the BTV serogroup. Genetic variability occurs within a group due to genetic drift of individual gene segments and genetic reassortment when ruminants or other vectors are infected with more than one strain.

Abstract

Blumea lacera (Burm f.) DC. belonging to family Asteraceae is an annual herb with a strong odour. This herb is distributed throughout the plains of North-west India upto an elevation of 2,900 m and is also reported from Tropical Africa, South East Asia, Bangladesh, Nepal, Indonesia, Malaysia, Philippines, Thailand and Vietnam. Blumea lacera is a plant with camphor-like odour, and is highly variable, grows in grassland, field, roadside and forest edge. Traditionally, it is used internally and externally as a hemostatic and anti-inflammatory agent. The juice of leaves along with black pepper (Piper nigrum) is given to cure bleeding piles. This herb is also offered as anthelmintic (especially for threadworm). Leaves and roots are used as astringent, diuretic and febrifuge and even in cuts and wounds. Bruises and ulcers can also be treated by applying fresh juice or extract. For treating piles (Hemorrhoids), the leaves of B. lacera has been used in ethnomedicinal practices.The phytoconstituents present in leaves are mainly terpenoids, glycosides, volatile oils, some glycoalkaloids and many more yet to be determined. B. lacera has been used as an ingredient in many pharmaceutical preparations. The chapter gives a brief idea about the ethnomedicinal usage, phytochemical profile, pharmacological usage and the future perspective of B. lacera. and would help the researchers for better understanding of this plant as well as developing plant derived molecules in drug discovery process.

In most of the examinations, the questions related to boat and stream are asked. These questions are seemed to be very difficult but are very ticklish and easy to solve. The students need to concentrate on flow of stream upward and down ward. If the boat is moving towards the flow (downstream), the speed of boat and stream will be added and if the boat is moving towards opposite of stream flow (upstream), the distance covered by the baot will be equal to boat speed minus strean speed. Few important tips are given below to solve such type of questions.

Downstream and Upstream Motion

Downstream motion of a boat is its motion in the same direction as the flow of the river. Upstream motion is exactly the opposite. There are two parameters in these problems.

1. INTRODUCTION

Water is the source that upholds every part of life on earth and is a key element of sustainable development. It is essential to the survival of healthy and safe lives of mankind. Ecosystems are inevitably linked with water. Lack of access to ample and safe drinking water is a serious setback among human population. Noxious substances from industries when enter into lakes, streams, rivers, oceans, and other water bodies, they get dissolved or lie suspended in water or get deposited on the bed. This results in the pollution of water whereby the quality of the water deteriorates, affecting aquatic ecosystems.

The strain of escalating population, growth of industries, urbanization, energy intensive life style, loss of forest cover, lack of environmental awareness and implementation of environmental rules and regulations, environment improvement plans, untreated effluent discharge from industries and municipalities, use of non-biodegradable pesticides/fungicides/herbicides/ insecticides, use of chemical fertilizers instead of organic manures, etc are basis of water pollution. The pollutants from industrial discharge and sewage, besides finding their way to surface water reservoirs and rivers are also percolating into ground to pollute ground water sources, threatening food security, access to safe drinking and bathing water and providing a major health and environmental management challenge. Contaminated water from inadequate waste water management provides one of the greatest health challenges restricting development and increasing poverty through costs to health care.

Water pollution is a sensitive problem all over the world. In the wake of increasing urbanization and industrialization, the pollution potential is gaining thrust day by day. Monitoring of waste water quality parameters is currently a subject of growing concern globally. Oxygen, although poorly soluble in water, is fundamental to aquatic life. In the absence of free dissolved oxygen, aquatic systems become inhabitable to most aquatic flora and fauna. Because the solubility of oxygen in water is so low, even little decrease in dissolved oxygen levels can compromise the health of natural water systems. Organic pollutants requiring oxygen for their decomposition exert a rapid and direct influence on the ecology of these environments. Therefore it is required to monitor the load of organic pollutants in the waste water.

Body condition scoring (BCS), although subjective in nature, is the only practical method of evaluation of body energy stores in dairy cows. The method of body condition scoring, originally proposed by Edmonson et al. (1987) and recommended by the NRC (2001) is described here. This method uses a 5 point scale, with BCS of 1 being extremely thin and a score of 5 being extremely fat. This system includes a combination of both visual appraisal and manual palpation on 8 separate body locations. Figure 1 shows the suggested BCS chart based on these locations. The assessor can score the cows using this chart with a fair accuracy, without much previous experience of scoring the cows. The scoring method is recommended for HF and Holstein crossbred cows.

Loss of BCS is expected during early lactation when a cow is mobilizing body fat in support of energy needs for lactation. Typical observed changes in BCS range from 0.5 to 1.0 condition score units during the first 60 days postpartum. A 1-unit decrease in BCS for a cow weighing 650 kg at calving (BCS 4) would provide 698 MCal of ME.That amount of ME is sufficient to support 564 kg of 4 per cent fat corrected milk.

The body condition scoring (BCS) is a subjective estimate of the energy reserves in adipose tissues of an animal. It is an acceptive, non-invasive, quick and inexpensive method to estimate degree of fatness. It is a means of accurately determining body condition of dairy cows, independent of body weight and frame size. It is evinced that the profile of lactating cows in milch barn is different e.g. some may be in early lactation and the others may either be in mid or late lactation. The observations on body condition status of animal will help the manager to adopt corrective management measures, thus enabling to improve the production profile of an animal. Hence, body condition scoring may be used as an efficient tool in this regard. Although many may consider this as a nutritional management practice, but changing BCS have implications on milk yield, health, reproduction, longevity and overall profitability of an animal. Body Condition Scoring (BCS) is a system used to evaluate the overall health and nutritional status of animals by 

Water : Importance of Water in Physiological Functions of the Body

A large proportion of the body of mammals including domestic animals, is made up of water. This water is an essential attributes for the life of the animals. Animals deprived of food but provided water will survive much longer than the animals provided with food but deprived of water. Water is the basis of the liquid environment of the cells, which Claude Bernard called the milieur interieur, and Cannon the fluid matrix. Its most important biophysical qualities are its solvent properties, its ionization and its heat of vaporization. Water is essential for metabolic processes, as most of these reactions must occur in aquatic environment. Water provides the medium for the transport of nutrients from their sites of absorption to the site of metabolism. Water also provides the medium for the transport of waste products from their sites of production to their sites of final excretion. Together with the main electrolytes, water forms an excellent buffering medium. Moreover, water is an essential substrate or end product of many biological reactions. Water due to its high latent heat of evaporation or fusion is an excellent insulator for the loss of body heat and is essentially required for the loss of heat to maintain body temperature, through the evaporation of water from the skin and the respiratory tract. Water also acts as a vehicle for the transportation of nutrients, hormones, waste products and gases etc.

Body water is derived from several sources which include the water drunk, intake of water through food and metabolic body water. Water is lost from the body through several routes which include loss of water through the feces and urine, evaporation of water from the skin and respiratory tract.

The concentration of water in the body varies from one tissue to another, being least in the dentine of teeth and greatest in the grey matter of the brain. The amount of water in the body also varies considerably with the age of the animal. The younger animals contain considerably more water than the older animals. This effect is mostly due to increase in the fatty tissues in the body, which contains relatively much less water, as the relative composition of tissues of the body other than fat remains relatively constant. The proportion of water in ruminants is higher as compared to nonruminants.

The two different compartments of the body fluids are extracellular fluid (ECF) and intracellular fluid (ICF) with variation in solute concentrations.
The extracellular fluid is the fluid in the cardiovascular system or in between the cells, called interstitial fluid.

The animals, which are aquatic cold blooded respires with the help of gills and swim with the help of fins such animals are called as fishes. Fishes have various body forms according to the environment and in changing the life of fish the body form of fish are found. The fish swim in water with the help of fins. The fins are present on all body of the fish. In swimming the body form is also important. Following are the various body forms found in fishes.

Body Forms

Following are some of the body forms in fishes:

1. Anguilliform body from:- When in fishes the body form is the anguilliform shape and size, like that of eel or anguilla type, then such body form is called as angilliform body form.

2. Depressiform body form :- When in the fishes body form is flattened from above downwards, means the body form is depressed dorso-ventrally it is called as depressiform body forms like that of angler fish, ray fish is also called dorso-ventrally depressed.

3. Disciform body form :- When in fishes the body form is flattened form sides or the fish is laterally compressed on both sides that type is called as disciform body form of laterally compressed body form like that of flat fishes.

Grasshoppers are highly voracious herbivores having the world wide distribution. It is particularly found across — Asia, Africa, Europe and Australia. In India it may be seen in abundance during monsoon season. This pest is of International importance and the following three species are commonly found in India of which desert grasshopper is predominant all over the country.

Centre of gravity

It is most commonly located is the middle of the rib cage, just caudal to the line separating the cranial and middle third of the body because the CG & located more cranially. The forelimbs bear 60-65% of body weight. This causes an increased stress of forelimb and increase incidence of lameness. The horse that is taller over the group than the wither has an additional disadvantage, because PTS CG is shifted further forwards.

1. Long backed horses may develop a swing in the gait that alters the movement of their limbs. Such horses are prone to speedy cutting. Cross firing & back problem due to muscle ligament strain.

2. Short backed horses may develop a swing in the gait that alters the movement of these limbs. Such horses are prone to speedy cutting, cross firing & back problem due to muscle and ligament strain.

1. Bone grafts

Bone grafting represents one of the earliest devised reconstructive approaches to the musculoskeletal system and remains among the most commonly used orthopedic procedures. Bone is the second most frequently transplanted tissue. The first recorded attempt to use bone graft is by the Dutch surgeon Job Van Meek’ren in 1668.

Definition

A graft is a living tissue that is transplanted to another site either within the same individual or into another individual for repair of a defect. An implant is a non viable material placed in a living system. Implants are composed of bone cement, metal or ceramic material. Grafts are structurally important for mechanical stability, but their use evolved to include the stimulation and enhancement of bone in response to injury.

Indications

Bone grafts are used, in general as framework, both to provide stability and to augment healing. It is used in the management of non union, delayed union, treatment of pseudoarthrosis, filling of osseous defects or cavities, arthrodesis, stabilization of spinal segments and addition of bone stock in total joint displacement.

Bone marrow is a soft, gelatinous tissue that fills the medullary cavities of bones. It plays a crucial role in the production of blood cells. It makes up about 3% of body mass in rats, 2% in dogs, and 5% in humans. Bone marrow develops in the embryo during the second trimester of pregnancy. This tissue is vital because it contains stem cells that can differentiate into various cell types. Bone marrow is of red and yellow types. In young mammals, the marrow is red and active (with minimal fat). With aging, accumulation of adipose tissue, and long bones transition turn bone marrow to yellow, fatty, inactive. In adult mammals, active hematopoiesis continues in flat bones, such as the sternum, ribs, pelvis, vertebrae, and skull, as well as the epiphyses of long bones. Erythropoiesis in these regions contributes significantly to red blood cell production, with the axial skeleton accounting for about 35% and the appendicular skeleton for 65%. In birds, hematopoiesis is mainly restricted to bone marrow, while in reptiles, it occurs in the bone marrow, spleen, liver, and thymus. Indications

In 1965, Urist demonstrates that the implantation of demineralized bone at extra skeletal sites induced de novo formation of cartilage and bone and proposed that the decalcified bone matrix possess high osteoinductive property and the inductor is a unique chemical that differentiates mesenchymal type cells (pericytes) into new bone. Urist and Starates (1971) reported that the inductor is a protein and termed it bone morphogenetic protein (BMP) . BMP is the bone induction principle.

Sources: Bone morphogenetic protein isolate from bovine as well as human bone and designated as bBMP and hBMP respectively. The molecular cloning of the bone morphogenetic proteins and their subsequent expression in recombinant system, resulted recombinant bone morphogenetic protein designated as rhBMP. BMP has been extracted from dentin and bone matrix, as well as from bone tumours, by enzymatic and nonenzymatic methods. The sources of BMP, thus far explored are rabbit dentin, rat, rabbit and bovine bone, human bone, mouse or human osteosarcoma and human placenta.

BMP circulates in the blood and it can be detected by BMP-radioimmunoassay. BMP levels higher in growing children (20-72 ng/ml) and patients with Paget’s disease (over 259 ng//ml) than in normal adults (14-18ng/ml). Crude and partially purified BMP has been extracted with the aid of collagenase, ethylene glycol, calcium chloride urea and Guanidine hydrochloride (GuHCL) solution. Wang et al (1988) purified BMP factor that induces bone formation> 300,000 fold from Guanidine chloride extracts of demineralized bone. As littlie as 50ng of this factor can induce the formation of cartilage and bone in an in vivo assay, though only 40µg could be obtained from 400kg of bovine bone powder.

DIGITS

Four digits are presents in the ox of these two the third and forth -are fully developed and have three phalanges and three sesamoid each.The second and fifth are vestiges.

FRIST PHALANX- (Fig.No.1)

The interdigital surface is flattened and its volor parts bears a prominence for the attachment of the interdigital ligaments.

The proximal extremity is relatively large and somewhat compressed from side to side.

The articular surface is concave from before backword and is divided by sagittal groove in two areas, of which the aboxial one is the larger and higher.

Behind tese are two facets for articulation with the sesamoid bones.

The distal extremity smaller than the proximal.

Aim

To know the various level of fracture & their appropriate method of immobilisation for large animal fracture.

Definition

Fracture is a disolution of bony continuity with or without displacement of the fragments.

Bone

Bones provide the rigid framework of the body of animals and man. The skeletal system serves three main functions. (1) It supports and protects the soft tissues and organs of the body. (2) It helps to maintain, through homeostatic regulation, the constancy of the ionic environment within the organism. (3) It helps in locomotion with the help of muscles and tendons.

The ends of long bone is called the epiphysis: the long, compact shaft, the diaphysis where it joins the epiphysis, the metaphysis. The diaphysis is composed of a hollow cylinder which contained the medullary cavity and bone marrow. During postnatal life, erythropoiesis occurs mainly in the bone marrow. In the adult, bone contains on wet weight basis approximately 25% water, 45 Percent ash and 30 percent organic matter. With increasing age, the ash content progressively increases as the water content decreases; the organic fraction remains relatively constant. Calcium constitutes about 37% of the ash content, and phosphorus about 18.5%.

Bone is an integral part of musculo-skeletal system of body. In the living animal it is bluish pink, whereas, fresh dead bone is yellowish-white in colour. It is hard and resistant to pressure but also have certain amount of elasticity. Its tensile strength is equal to the cast iron, though bone three times lighter and ten times flexible to cast iron.

Number of bones: The number of bones of the skeleton varies with age and species. Number of bones in cattle-210; Horse-208; Dog-271; Fowl-166 and human-208 (207-212)

Composition of bone: Bone contains different bone cells, organic and inorganic matrix and water. Organic matrix is about one-third and Inorganic matrix is two-third weight by volume. The organic material consists of 90% collagen [type-I (maximum), type V and type XII] and 10% non-collagenous glycoprotein and bone specific proteoglycans (osteocalcin, osteonectin, sialoprotein, bone phosphoproteins and small proteoglycans) and bone growth factors [TGF-beta family; Insulin-like growth factor-1, 2; bone morphogenetic protein (BMP); platelet derived growth factor (PDGF); Interleukin -1 (IL-1); Interleukin-6 (IL-6); colony stimulating factor]. Organic matrix of bone resembles the matrix of dense fibrous tissues such as tendons, ligament and joint capsule. It is not certain whether all of these proteins are synthesized by bone cells or whether they are synthesized by cells outside of the bone and then incorporated into the bone matrix. However, their presence within bone and their potential to affect the activity of bone cells suggests they have important role in controlling bone cell functions. Inorganic matrix of bone or mineral component of bone serves as an ion reservoir and gives bone of its stiffness and strength. It reserves 99% of body calcium, 85% body phosphorus and 40-60% body sodium and magnesium. Inorganic matrix of bone is composed of 85% calcium phosphate, 12% calcium carbonate, 3% of magnesium phosphate, calcium fluoride and magnesium carbonate. It also contains traces of chlorine, sodium, potassium. These minerals remain as hydroxyapatite crystal form within bone. However, bone mineral crystal is not pure hydroxyapatite, instead they contain both carbonate and acid phosphate groups (HPO₄) and bone crystal does not contain OH groups. Bone matrix has such great durability and stability that it can remain essentially unchanged and retain much of its strength for centuries after death. Removal of organic matters renders the bone fragile, brittle and shattered, whereas, decalcification renders bone soft and pliable and resistant to fracture.

FEMUR

The femur has a relatively small shaft, which is cylindrical in its middle. The trochanter minor has the form of a rough tuberosity.

The trochanteric ridge connects it with the trochanter major. The third trochanter is absent. The supracondyloid fossa is shallow. The proximal extremity is very wide.

The fovea capitis is a small depression on the middle of the heacKfor the attachment of the round ligament.

The trochanter major is very massive and is undivided; its lateral surface is very rough. The trochanteric fossa is deep. The ridges of the trochlea are less oblique.

The ilia are almost parallel to each other and form a much smaller angle with the horizontal plane than in the horses.they are relatively small. The glutei line is prominent and nearly parallel to the lateral border,it joins the ischiatic spine.A rounded ridge separates the two parts of pelvic surface.The surface for articulation with sacrum is triangular. The tuber coxae is relatively prominent and not so oblique as in horses, wide in middle,smaller at either end.Shaft is short and compressed from side to side.

Ischium is large.Its long axis is directed obliquely upward and downward forming an angle of 5o to 60 degree with horizontal plane.The transverse axis is obliquely downward and inward at similar angle so concave from side to side.Ventral surface has rough ridge for muscular attatchment. The ischiatic spine is high and thin and bears a series of vertical rough line. lschiuatic arch is narrow and deep.The tuber ischii is large and three sided bearing dorsal ,ventral, lateral tuberosities.The symphysis bears a ventral ridge which fades out at ischial arch.The acetabular branch of pubis is narrow and is directed laterally,and a little forward.The anterior border is marked by a transverse groove which ends below rough iliopectineal eminence. The symphyseal branch is wide and thin. The acetabulum is smaller than in horses. Rim is rounded and usually marked by two notches. One is directed to deep acetabular fossa and and converted to foramen. The other is anteromedial and small also replaced by foramen or absent.

Bones of the animal body makes a skeleton system and helps in locomotion and support soft tissues. The proportion of bones in animal body varies with the animal species as they contribute 12-28 percent in cattle and buffalo, 17-35 percent in sheep and lambs, 50percent in bobby calves, 25 percent in veal calves, 12-20 percent in pigs and 8-17 percent in poultry. As the age advances bones becomes rigid due to ossification of cartilages but in older age bones becomes brittle and fragile due to reduction in bone organic matter.

Bonsai is a Japanese art form using miniature trees grown in containers. The first documented evidence of an oriental word for container planting emerged approximately 1600 years ago in China. Bonsai is a Japanese pronunciation of the earlier Chinese term pen zai. A bon is a tray-like pot typically used in bonsai culture. The purposes of bonsai are primarily contemplation (for the viewer) and the pleasant exercise of effort and ingenuity (for the grower). Bonsai is not intended for production of food, for medicine, or for creating yard-sized or park-sized gardens or landscapes. Instead, bonsai practice focuses on long-term cultivation and shaping of one or more small trees in a single container.

The practice of bonsai is sometimes confused with dwarfing, but dwarfing more accurately refers to research and creation of plant cultivars that are permanent, genetic miniatures of existing species. Bonsai does not require genetically dwarfed trees, but rather depends on growing small trees from regular stock and seeds. Bonsai uses cultivation techniques like pruning, root reduction, potting, defoliation and grafting to produce small trees that mimic the shape and style of mature, full-sized trees.

Bonsai can be created from nearly any perennial woody-stemmed tree or shrub species. Which produces true branches and can be cultivated to remain small through pot Some species are popular as bonsai material because they have characteristics, such as small leaves or needles that make them appropriate for the compact visual scope of bonsai. Some of the plants highly suited for making bonsai

Bonsai culture is an important branch of Ornamental horticulture. History of Bonsai is very old dating back to many centuries in China where it originated, as most Chinese arts and from there it spread to Japan and became highly specialized.  In fact, bonsai culture is in the present day more known as Japanese than as a Chinese art.  In Japanese homes, there are plants which are hundreds of years old. This treasured art is passed from generation to generation in Japanese society.

The word Bonsai is a combination of two Japanese words, Bon (meaning shallow pan) and Sai (meaning plant) which can be interpreted as ‘Tray planting’.  The idea conveyed is that of a small beautiful tree growing in a container as in nature with all its parts performing their natural functions throughout the times.  The artistic look of plant in a miniature form maintained for many years is the prime criteria.  Bonsais are grown outdoors and are displayed in living room, as and when, needed.  They cannot be treated as house plants.

Bonsai is the art and science of dwarfing plants and growing them in shallow containers. The word ‘Bonsai’ is a Japanese term consisting of two words, ‘Bon‘- a shallow container and ‘sai’ - to plant. Therefore, bonsai is a technique of growing plants (trees, shrubs and others) in shallow containers in dwarf form. With the help of pruning, pinching and training a three dimensional effect is produced. It is a kind of living sculpture and idealized nature in miniature. An ideal bonsai is replica of a particular plant or an artistic variation in shape whose different parts are in complete harmony. Development of bonsai requires an artistic eye, skills on training, pruning and knowledge on growing plants in containers.

This technique was developed by the Chinese over 2000 years ago and later excelled by the Japanese. Bonsai is now popular all over the world and very useful for the people who are living in flats and have no scope to have outdoor gardens. Every house owner likes to have few specimen plants for interior decoration. Bonsai provides them an additional opportunity to grow more number of plants being dwarf in nature. Considering its present popularity, Bonsai is now important horticulturally for its commercial and aesthetic value.

Bonsai is an art of growing and training of a woody plant in a miniature form in a shallow container imitating natural look of an old aged tree. It differs from a pot plant where either the foliage or the flower is the main criterion, but here the artistic look of the tree in a miniature and natural form maintained for many years is desired.

The word Bonsai’ is a combination of two Japanese words, Bon (meaning shallow pan) and Sai (meaning plant), which can be translated as “tray planting”. By growing the trees in shallow containers, the growth is checked or dwarfed but the plant is not starved. A properly maintained bonsai should outlive a full size tree of the same species. Bonsai is a small tree in comparison to its huge life-sized tree of the same species. Thus, Bonsai is specially trained ordinary tree or plant, not special hybrid dwarfs. Bonsai culture is viewed as a hobby that allows greater understanding and association with nature. This art of bonsai is linked with Zen Buddhism - where this art originated as pastime that merged with natural elements and developed into a unique method of meditation and expression. The bonsai is even expressed as “heaven and earth in one container”

Bonsai is developed from seeds, cuttings, layering or grafting from young trees or from naturally occurring stunted trees transplanted into shallow containers. Most bonsai range in height from 5 centimeter (2 inch) to 1.2 meter (4 ft). Bonsai is kept small and trained by pruning branches and roots, periodic repotting, pinching off new growth and wiring the branches and trunk for giving desired shape. Tradition holds that three basic virtues are necessary to create a bonsai: shin-zen-bi standing for truth, goodness and beauty which are parallel to Hindu concept of Satyam, Shivam, Sundaram. The bonsai must look natural and should never show the intervention of human hands (with the exception of Chinese bonsai which in many cases depicts images of dragons and other influential symbols of the culture at the time of origination). Bonsai is primarily kept outdoors in partial shade with exception of some plants suited, trained and indoor grown. In traditional Japanese rooms, there is a tokonoma, the alcove, specially designed for the display of bonsai on special occasions. Bonsai can live for hundreds of years with utmost care and the prized specimens are being passed from generation to generation, admired for their age and valued as a reminder of those who have cared for them over the centuries.

I. General information

1. Bonsai is defined as Japanese art of growing trees & shrubs by extreme dwarfing in a shallow container
2. Bonsai, the name is derived from two words, Bon & Sai
3. The meaning for the word ‘Bon’ is tray or shallow container
4. The meaning for the ‘Sai’ is to grow
5. Origin of Bonsai is china
6. In Mamie bonsai, plant height is 2-6 inches
7. In small bonsai, plant height is 6-12 inches
8. In medium bonsai, plant height is 12-24 inches

Definition: Bonsai is an art of growing dwarfed, ornamentally shaped trees or shrubs in shallow containers or trays. It’s an art that expresses in miniature form of a natural tree. The word Bonsai is a Japanese term which literally means “planted in a container”.

This art form is derived from an ancient Chinese horticultural practice, part of which was then redeveloped under the influence of Japanese Zen Buddhism. Thus, origin of bonsai culture is China. Though it was popularized by Japanese. The ultimate goal of growing a bonsai is to create a miniaturized but realistic representation of nature in the form of a tree. In Japan bonsai is not only a hobby but also act as status symbol.

Bonsai is an astonishing art-form, combining horticultural approaches and Asian aesthetics in a distinct way, which reflects the nature’s beauty (Pietraszko and Sobota 2008). The art of bonsai is considered to be originated in Chinese empire (Doyle et al., 2019) and was later adapted by the Japanese, where it has become the integral part of their culture (Ching 1990, Roth 2014). The word ‘Bonsai’ is derived from two Japanese words ‘Bon’ (Shallow pan) and ‘Sai’(plant) which literally means “planted in a container”. The Chinese started creating miniature landscapes in the form of Bonsai during Han Dynasty over 2000 years ago. During the Kamakura period (700 years ago) the Japanese adopted the bonsai art-form and subsequently a different Japanese style form came into existence. This art of bonsai was further redeveloped under the influence of Japanese Zen Buddhism (Doyle et al. 2019).

A bonsai is a nature’s replication of plant in its miniature form, without depicting the human interference too clearly. Thus, they are normal trees and shrubs which are dwarfed by a specific system of pruning-training-wiring. Techniques such as pinching buds, pruning and wiring branches, and carefully restricting but not abandoning fertilizers are used to limit and redirect healthy growth in bonsai. They are different from the pot plants which are mainly grown for either their foliage or floral beauty, but in case of bonsai the whole artistic look of the plant in its miniature form is maintained for years. Bonsai can be grown both outdoors and indoors, especially they are ideal for display in living rooms, verandas, terraces and window-sills etc. The good bonsai specimens are usually hardy species that can be kept outdoors the year round wherever winters are mild. They can be brought into the house occasionally for appreciation and enjoyment. In Japan, they are customarily displayed in a recess area or on small tables in a living room and later returned to their outdoor bonsai stands.

LIVESTOCK ECONOMICS, MARKETIG, ACCOUNTANCY & ENTREPRENEURSHIP DEVELELOPMENT IN ANIMAL HUSBANDRY & DAIRYING

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Introduction

We, the participants of ITAF2023 with Dr. B. R. Chavan and Dr. Prashant Bodake, visited Boonsawang Co. under the guidance of Asst. Prof. Dr. Naphat Panthukumbol and Mr. Suthi Mahalao on 18/10/2023 at 09:00 am. Boonsawang Co. in Chonburi, owned by Suthi Mahalao, also exports fry to Vietnam, Malaysia, Singapore, Taiwan, Australia, and Israel. In addition, Suthi runs his sea bass farm and is a feed distributor to farms in the province. The purpose of the visit was to learn about the seabass culture system and get familiar with technical aspects. The farm occupies an area of 10 hectares and is covered in 11 ponds. The farm is aimed to grow from fry to marketable size seabass. The single crop duration is divided into 4 periods.

Abstract

This chapter will look at the major reasons why farmers in India are unable to take advantage of Agricultural profession as a mode of generating income and suggest ways to change this by employing new technologies. Agriculture has basic processes which require identifying the season for planting the seeds at the right time, watering the plants to the required level, undertaking maintenance for the different plants and then finally harvesting at the right time to ensure that the effort that has been put into the agricultural process results in useful output. In business terms the output when it is valued in terms of money, should enable the farming business to sell their output at a certain price that will enable them to pay off the money that has been used for setting up the crop for that cycle. This chapter will underscore the valuable contribution of remote sensing data in farming to enhance operational decisions. We will look at how low-cost technologies can be implemented into the existing agricultural extension activities and help farmers make better decisions that will enable them to be more business oriented. It is envisaged that incorporating high technology-based data into simple to understand terminology willhelp reduce the burden on farmers. This is due to the lack of knowledge but also due to the low volume of information that is possible to be fed to the farmers through the agriculture extension network in the country. However, due to the varied nature of farming in India it is difficult for extension officers to specialise but concentrate their efforts on widely utilised crops in an area. The following figure below explains in simple terms how the cycle of activities occur in a farmer’s world and where business decisions are taken that will have an impact on the profitability.