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The poultry industry in India is experiencing rapid growth, with meat production and egg production increasing by 5.13% and 6.77%, respectively, in 2022-2023 compared to the previous year (BAHS, 2023). Numerous factors and challenges impact poultry production on a global scale, such as intense international competition, evolving consumer attitudes toward food safety, animal welfare, and environmental conservation. Poultry commonly harbor infectious diseases that can also affect humans, with many of these zoonotic diseases having reservoirs in mammalian species other than humans, adding complexity to their management.

Phytobiotics are plant derivatives such as herbs, plant extracts or spices and have a wide range of activities such as stimulation of feed intake, growth and endogenous secretions in the gut. They act as immunomodulators resulting in decreased mortality and also have coccidiostatic, anti microbial, anthelminthic and anti-inf lammatory activities. Phytobiotics also possess hepatoprotective and hepatogenic properties, which tone up liver resulting in increased nutrient utilization and better performance. Herbs like Achyranthes aspera (Prickly Chaff Flower, Devil’s Horsewhip, Apamarga), Andrographis paniculata (Green chirayta, King of bitters, Kalamegha), Azadirachta indica (Neem), Boerhaavia diffusa (Spreading Hogweed, Punarnava), Eclipta alba (False Daisy, Bhringaraj), Ichnocarpus frutescens (Black creeper, Utpalagopa), Terminalia chebula (Black myrobalan, Haritaki). These herbs have hepatostimulant, hepato-protective, immunomodulatory and antioxidant activities (Sadekar et al., 1998; Manu and Kuttan, 2009; Michels et al., 2011, Dash et al., 2007). Further, they optimize digestion and metabolism resulting in better protein utilization, improved mucosal function and reduced cost of metabolic deamination. Andrographolide and 14-deoxy-11, 12-didehydro-andrographolide isolated from Andrographis paniculata inhibits free radical activities and lipid peroxidation. Inhibition of lipid peroxidation in meat prevents free radical production thereby preserving meat composition, colour and improvement in shelf life. In addition, it has been studied that a herb Terminalia chebula helps to reduce stress (Selvakumar et al., 2007).

1. Introduction

Food poisoning is a major concern for both the food industry and consumers inspite of different preservation practices some species of pathogenic bacteria causes food spoilage leading to outbreak of illness. Spices and herbs have been added to food since ancient times, not only as flavouring agents, but also as food medicine and as food preservative (Nakatani, 1994). Additives need for food preservation may be harmful to human health and has led to use of natural products especially plant extracts, their essential in food preservation.

More than 90 percent of the cases of food poisoning each year are caused by Staphylococcus aureus, Salmonella typhi, Vibrio cholerea, Proteus mirabilis, Pseudomonas aeruginosa, Clostridium perfringens, Campylobacter, Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus and Entero-pathogenic Escherichia coli. These bacteria are commonly found on many raw foods and cause severe disorder in humans. Spice posses anti adhesive properties that prevent the microbe to the host tissue, thus preventing the primary infection. It also possesses a rich array of phytochemical and a rich source of antioxidant. Some studies claim that the phenolic compounds present in spices and herbs might also play a major role in their antimicrobial effects.

1. Introduction

Medicinal plants are rich source of novel drugs that forms the ingredients in traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk medicines, pharmaceutical intermediates, bioactive principles and lead compounds in synthetic drugs (Ncube, 2008).  In recent years, antibiotic resistance has become a global concern. This drives the need to screen medicinal plants for novel bioactive compounds as they are biodegradable, safe and have fewer side effects (Prusti et al., 2008). Higher plants, as a source for new potential drugs is still largely unexplored and screening of active agents helps to design the lead molecule later in drug development.

Aegle marmelos(L.) Corr., belongs to the family Rutaceae, and is popularly known as Vilvam tree (Tamil) (Gamble, 1935; Mathew, 1983). It is an indigenous tree found in Iran, India, Myanmar, Pakistan, Bangladesh and most of Southeast Asian countries (Singh, 2009).  A. marmelos has been used from time immemorial in traditional systems of medicine for relieving diarrhoea, dysentery, stomachic, digestive, astringent, peptic ulcer and respiratory infection (Nadkarni, 2000). The fruit, leaves, bark and root are edible and possess medicinal values. The unripe dried fruit used to be astringent, digestive and stomachic, the ripe fruit is aromatic, cooling and is used as laxative.

1. Introduction

Seaweeds are the extraordinary sustainable resources in the marine ecosystem which have been used as a source of food, feed and medicine. The number of natural products isolated from marine organisms increases rapidly, and now exceeds with hundreds of new compounds being discovered every year (Chapman and Chapman. 1980, Cheennubhotla et al., 1986; Chennubhotla et al., 1987; Faulkner, 2002; Proksch and Muller, 2006). Several products of algal origin such as alginate, carrragenean and agar as phycocolliods have been used for decades in medicine and pharmacy (Fitton, 2006). Seaweeds are considered as source of bioactive compounds and produce a great variety of secondary metabolites characterized by a broad spectrum of biological activities.  Seaweeds contain various inorganic and organic substances which can benefit human health (Kuda et al., 2002). The in vitro studies on green, brown and red algae confirmed the following potential activities viz., cytostatic, antiviral, antihelminthic, antifungal and antibacterial (Lindequist and Schweder, 2001; Newman et al., 2003; Dhargalkar and Neelam, 2005; Huang et al., 2005; Gonzalez del Val et al., 2001; Espeche et al., 1984).

1. Introduction

Medicinal plants are nature’s priceless gift to human. They have been used all over the world for the treatment and prevention of various ailments particularly in developing countries where infectious diseases are endemic and modern health facilities and services are inadequate. Medicinal principles are present in different parts of the plants such as root, stem, bark, heartwood, leaf, flower, fruit or plant exudates (Thenmozhi et al., 2010). Phytochemicals from medicinal plants serve as lead compounds in drug discovery and design (Sofowora, 2006).

Free radicals are chemical species which contain one or more unpaired electrons, due to which they are highly metastable and cause damage to other molecules by extracting electrons from them in order to attain stability (Chakraborthy and Ghorpade, 2010). Hyper-physiological burden of free radicals causes imbalance in homeostatic phenomena between oxidants and antioxidants in the body. This imbalance leads to oxidative stress that is being suggested as the root cause of aging and various diseases like diabetes, cancer, and liver damage (in severe conditions like cirrhosis and hepatitis) (Wagh et al., 2010).

1. Introduction

Plant-derived substances have recently become of great interest owing to their versatile applications. Medicinal plants are the richest bio-resource of drugs of traditional systems of medicine, modern medicines, nutraceuticals, food supplements, folk medicines, pharmaceutical intermediates and  chemical entities for synthetic drugs Medicinal value lies in certain chemical substances that have its physiological action on the human body. Many indigenous medicinal plants are used as spices and food plants. The general techniques of medicinal plant extraction include maceration, infusion, percolation, digestion, decoction, hot continuous extraction (Soxhlet), aqueous-alcoholic extraction by fermentation,  counter-current extraction, microwave-assisted extraction, ultrasound extraction (sonication), supercritical fluid extraction, and phytonic extraction (with hydrofluorocarbon solvents). For aromatic plants, hydrodistillation techniques (water distillation, steam distillation, water and steam distillation), hydrolytic  maceration followed by distillation, expression and enfleurage (cold fat extraction) may be employed.

ABSTRACT

Advances in biotechnology, particularly methods for culturing plant cells and tissues can provide new means for the commercial processing of even rare plants and th chemicals they produce. These new technologies will extend and enhance the usefulness of plants as renewable resources of valuable chemicals. Aristolochic acid is mainly found in the genus Aristolochia. Aristolochic acid has been shown to hav anti-tumour activity and has been recommended for its activity against various effect of snakebite and its capacity to inhibit phospholipase A2 or other enzymes Considering the importance of medicinal use of A. bracteolata and A. indica, in th present study, they were selected for in vitro development and rapid multiplication o plant through tissue culture. The study was undertaken to enhance the in vitr production of Aristolochic acid in different tissue cultures. Cell suspension culture was established from friable calli of both the species in M liquid medium. Bergmann plating technique was carried out for cell line selection fo more production of Aristolochic acid. Primary transformation was carried out by Agrobacterium tumefaciens strain LBA4404 / pTOK233 with Gus and hpt genes to confirm the transgenic potential of A. bracteolata and A. indica. In order to enhance the productio of aristolochic acid in in vitro culture condition, the physical, chemical and biologica conditions were altered. Aristolochic acid was isolated, identified and purified from naturally growing plants as well as from tissue culture plants and all other cultur stages.

Abstract

Upcoming era of functional foods has led food scientists and food processors to deeply understand the role of phytochemicals in promoting human health. Phytochemicals are the secondary metabolites produced by plants and are commonly found in our daily food. Natural food products seem to be good substitute for pharmaceutical supplements to improve health status of people. Therefore, consumer's interest is growing in the food items naturally containing phytochemicals, each having there own functional and health aspects. Most discussed phytochemicals in the present paper are carotenoids such as lycopene, beta-carotene, lutein and, polyphenols such as phenolic acids, flavonoids, anthocyanins and isoflavones. Major dietary source for carotenoids include orange and yellow fruits and vegetables as well as green leafy vegetables whereas polyphenols are found abundantly in green tea, wine, berries, cocoa, broccoli. Carotenoids can also be used as colorants in foods during processing. The role of polyphenols as an antioxidant is well characterized because of there ability to scavenge free radicals or bind pro-oxidant metal ions by means of their hydroxyl group. Carotenoids also play an important role in protection against photooxidative processes by acting as singlet molecular oxygen as well as peroxyl radical scavengers, thus acting as potent antioxidant. Apart from antioxidative properties multitude of health benefits are associated with carotenoids and polyphenols such as reduced risk of age-related macular degeneration, certain cancers such as prostate, lung and colon, prevention of cardiovascular disease and enhancing immunity towards infection. Hence, continuous developments are being made to combine phytochemicals to avoid improper combinations that may nullify the effect of supplements and cause lower absorbance.

Introduction

Plants are the principal producers of phytochemicals, which are compounds with biological activity. Plants serve as the primary source of many active compounds in the pharmaceutical business. Their pharmacological character istics make them useful in the treatment of fungal and bacterial infections, as well as degenerative conditions such as diabetes and cancer that progress over time. Pharmacologically, it has specific effects on human health, including anti-inflammatory properties, antiallergic, antioxidant compounds, antimicrobial, antifungal, antispasmodic, chemopreventive, hepatic-protective, hypolipidemic, neuroprotective, hypotensive, antiaging, bone density loss, DNA damage, cancer, and heart diseases.

1. Introduction

Plant metabolites are the major sources of pharmaceutical food additives, fragrances and pesticides. It is estimated that the market potential for herbal drugs in the western world alone could range from US $ 4.9 billion to 47 billion by the year 2000 if the AIDS epidemic continues unchecked (Biotechnology and Development, 1991). A similar situation exists for plant based food additives, fragrances and bio-pesticides. The natural resources for plant based chemicals are limited but the consumer preference for natural products is greater. Alternative avenues for plant products have gained prominence during the past few years. In recent years, plant Biotechnology has gained considerable importance for the production of phytochemicals (Heble, 1996).

During the past three decades, several phytochemicals with biological activities have been identified from higher plants for plant tissue culture industry (Verpoorte et al., 1991). Vincristine, vinblastine, artemisinin, taxol and podophyllotoxin are in the list of approved drugs. Some products under clinical trials are forskolin, castanospermine, ginkgolides and hyperin (Heble, 1996). In recent years, there has been renewed interest in screening higher plants for novel biologically active products particularly to combat such ailment that have defined synthetics and antibiotics. This has resulted in the development therapeutics used in the treatment of cancer, hypertension, AIDS, malaria and other diseases.

Abstract

Recently there has been increasing interest in plants and plant-derived compounds as medicinal agents. In Ayurveda, a wide range of medicinal uses of Pterocarpus santalinus is described. Many important bioactive phytocompounds have been extracted and identified from the heartwood of P. santalinus. The major bioactive compounds present in the heartwood of P. santalinus are santalin A and B, savinin, calocedrin, pterolinus (K,L) and pterostilbenes. These phyto-compounds present only in small amounts and have more effect than nutrients. The heartwood of the plant is used traditionally for the treatment of inflammation, diabetes, headache, skin diseases, jaundice, and in wound-healing. A wide range of pharmacological activities and health benefits of P. santalinus have been reported, including antioxidative, antidiabetic, antimicrobial, anticancer, and anti-inflammatory properties, and protective effects on liver, gastric mucosa and nervous system. All these protective effects were attributed to bioactive compounds present in P. santalinus. Most of the secondary metabolites reported from this plant are terpenes, phenolics, flavones and sterols. The present communication gives the phytochemistry and pharmacological activities of P. santalinus on health with up-to-date information.

Phytochemistry

Pytochemistry is concerned with enormous variety of organic substances that are elaborated and accumulated by plants and deals with chemical structures of these substances and biosynthesis, and their natural distribution and biological function. Chemical constituents of plants can be classified in different ways. Based on biosynthetic origin, solubility properties and presence of certain key functional groups, it is classified as phenolic compounds, substances that are readily recognized by their hydrophilic nature and by their common origin from aromatic precursor shikimic acid. Terpenoids share lipid properties and a biosynthetic origin from isopentenyl pyrophosphate (Fig. 1). Organic acids, lipids, alkenes and related hydrocarbons, polyacetylenes and sulphur compounds are derived biosynthetically from acetate. Nitrogen compounds of plants like amino acids, amines, alkaloids, cynogenic glycosides, indoles, purines, pyramidines and cytokinins and chlorophyll are basic substances recognized by their positive responses to either ninhydrin or Dragendorff reagent. Monosaccharides, oligosaccharides, sugar alcohols and cyclitols are water-soluble carbohydrates and their derivatives. Macromolecules of plants such as nucleic acids, proteins and polysaccharides are easily separated from other constituents by their high molecular weights (Harborne, 1984). Some of the therapeutically active compounds are discussed below:

1. Introduction Plants naturally contain compounds called phytochemicals. Due to their numerous therapeutic applications, these phytochemicals are becoming more and more well-known these days. Phytochemicals are essential in the fight against a variety of illnesses, including cancer, arthritis, and asthma. These phytochemicals have no side effects like pharmaceutical compounds do. Because the phytochemicals treat illnesses without harming people, they can also be referred to as “man-friendly medicines.” The primary topics of this study are phytochemical collection, extraction, and qualitative and quantitative analysis. In order to protect themselves against herbivores, many plants secrete chemical substances. Examples of therapeutic plants that include the main groups of pharmacologically active phytochemicals are provided below. Weeds that contain phytochemicals, like chickweed, dandelion, and nettle, are frequently found surrounding human settlements. Many phytochemicals, including curcumin, epigallocatechin gallate, genistein, and resveratrol are pan-assay interference compounds and are not useful in drug discovery.

Introduction

Plant products have been used for centuries by humans as food and to treat ailments. Natural medicinal products originating from herbs and spices have also been used as feed additives for farm animals in ancient cultures for the same length of time. Keeping farm animals healthy is necessary to obtain healthy animal products. The main scope in animal husbandry is to ensure good performance of farm animals and get quality animal products, which can be achieved only with the effort to keep the animals healthy. Only quality feed together with proper hygiene, potable water and management can ensure the production of nutritious animal products with desired organoleptic properties (Saxena, 2008). A ban of antibiotics as feed additives in animal nutrition is realized since 1986 in Sweden and later on to various countries. A general ban is foreseen in some years from now, because of the increased occurrence of pathogens resistant against therapeutical antibiotics used in animals and humans. With the restricted use or ban of dietary antimicrobial agents we must explore new ways to improve and protect the health status of farm animals, to guarantee animal performance and to increase nutrient availability (Wenk, 2003). In this aspect, herbs and spices are not just appetite and digestion stimulants, but can, with impact on other physiological functions, help to ensure good health and welfare of the animals, what can positively affect their performance.

Aim

Phytosynthesis of silver (Ag) nanoparticles using Cassia roxburghii aqueous leaf extract.

Introduction

The Ag nanoparticles constantly exhibit adverse effects on microbes such as inhibition and inactivation (Nasrollahi et al., 2011). The Ag nanoparticles synthesized by using various biological sources such as herbs, plants and biological organisms display excellent properties in various fields i.e., non-linear optics and intercalation materials for electrical batteries as optical receptors, catalysts in chemical reactions biolabelling and as antibacterials (Sanghi and Verma, 2009).

1. Introduction
Aquaculture, the farming of aquatic organisms such as fish, shellfish, and aquatic plants, has become one of the fastest-growing food production sectors globally. This growth has been driven by the increasing demand for high-quality protein to feed a growing global population, coupled with the depletion of wild fish stocks due to overfishing. As the aquaculture industry expands, so too does the need for sustainable practices that balance productivity with environmental and animal welfare considerations. One of the critical challenges faced by modern aquaculture is the need to maintain fish health and growth performance while minimizing the industry’s ecological footprint. Traditionally, synthetic additives such as antibiotics, chemical growth promoters, and other artificial substances have been used to improve fish health, enhance growth rates, and prevent disease outbreaks. However, the excessive use of these chemicals has led to several issues, including antibiotic resistance, chemical residues in fish products, and environmental pollution. These concerns have spurred a shift toward more sustainable, eco-friendly alternatives in aquaculture production systems.
In this context, phytogenics—natural, plant-based additives—have garnered increasing interest as a promising solution. Phytogenics are bioactive compounds extracted from various plant sources, including herbs, spices, and essential oils, that possess multiple beneficial properties. These compounds have been used in traditional medicine and animal husbandry for centuries due to their antimicrobial, antioxidant, anti-inflammatory, and immunomodulatory effects. By leveraging the natural bioactive compounds in plants, phytogenics offer a range of advantages for aquaculture, including enhanced growth performance, improved feed efficiency, strengthened immune responses, and reduced disease susceptibility. The need for sustainable and natural alternatives to synthetic additives is becoming more urgent as the global consumer base becomes more health-conscious and environmentally aware. Consumers today increasingly demand seafood products that are free from chemical additives

Plant hormones - They are defined as:

1. Small
2. Organic compounds;
3. Synthesized by the plant;
4. Active in low concentration (<10^-6);
5. Promote or inhibit growth and developmental responses;
6. Often show a separation of the site of production and the site of action (although this isn’t as clear a distinction as in animals).

Based on these criteria, would the following substances be considered hormones? Ca²⁺, sucrose, 2 4 - D, glycine, or K⁺?

Plant diseases caused by variety of pathogens pose a serious challenge and economic threats to various agricultural crops all around the world. It has been evidenced that despite wide use of chemical pesticides in crop production, the losses due to pests and diseases are significant. Phytonematodes are one of the major groups of organisms that live in diverse habitat and continue to challenge variety of agricultural crops all around the world. Since early times the major agricultural crops have been plagued by these noxious nematodes that feed on various plant parts viz. roots, rhizome, sucker, tubers, seedlings, stem, leaves, buds, crowns and cause damage worth more than 100 billion dollars annually. The major crops severely affected by these pests are, vegetables, fruits, sugarcane, sugar beet, cotton, oil seed, pulses, tobacco, tea, coffee, cereals, spices, medicinal and aromatic plants.