eChapters

Policy plays a critical role in shaping the future of agricultural sciences. It determines the resources allocated to research, the inclusivity of publishing practices, and the extent of global collaboration (Fig. 15.1).
This chapter outlines actionable policy recommendations to support equitable publishing, strengthen research infrastructure, and encourage global partnerships. Furthermore, it delves into the importance of fostering interdisciplinary research and emphasizes why agricultural sciences need to continue gaining momentum in the face of global challenges.
15.1. Equitable Publishing Practices
Access to academic publishing remains a significant challenge for researchers in developing countries. High article processing charges (APCs) for open-access journals often exclude underfunded institutions and individual researchers. Policy interventions are essential to create a more equitable publishing ecosystem.
15.1.1. Subsidies for Open Access Publishing
Governments and funding agencies should subsidize APCs for researchers from low-income and middle-income countries (LMICs). For instance, the Plan S initiative-backed by organizations like the European Science Foundation—aims to make all publicly funded research open access (Plan S, 2018).

Drought, a sporadic yet impactful event, presents a significant challenge to agricultural systems and economies globally. A notable observation in this context is the tendency of policymakers to readily claim success for their governance during years of favorable weather, while conversely attributing agricultural failures in drought years primarily to natural causes. This inclination often masks an underlying lack of consistent focus and investment in proactive drought-proofing measures. This international comparison examines the contrasting approaches to drought management between developed and developing nations, highlighting how this lack of focus, coupled with varying capacities and resources, shapes their respective policy responses. Understanding these disparities is crucial for fostering more effective and equitable drought resilience measures worldwide, moving beyond the simplistic attribution of agricultural successes to policy and failures solely to weather

Pollination

Pollination is the act of transfer of pollen from the male reproductive organ of a plant to the female reproductive organ of same or another plant.

Pollination is of two types

5.1 Self Pollination

It is the transfer of pollen from the anther to the stigma of the same flower from where the anthers have been generated. Thus, self pollination happens to be denominated as autogamy where auto means “same” and gamy means “union”. Obviously, self pollination is found in bisexual flowers. But self pollination also occurs when pollen is transferred from one bisexual flower to the stigma of another bisexual flower of the same plant. This kind of autogamy is known as geitonogamy. So, self pollination has to be defined keeping the parent plant on the main focus not the flower. If genetical consequences are considered, self pollination promotes the occurance of purelines or homozygotes. Self pollinating species developed themselves evolutionary fit due to homozygous balance and do not exhibit significant inbreeding depression.

7.1 Introduction

Breeding plans are based on controlled mating (i.e., mating between selected strains) and selfing of selected strains or a combination of both. Continued selfing is practised to isolate purelines and inbreds. On the other hand, crossing (hybridization) is necessary to produce hybrids and synthetics. In crossing, self-pollination is prevented in the female parent. Male sterility and self-incompatibility easily exclude self-pollination, and so they are used in hybrid seed production.

7.2 Male Sterility

Koelreuter (1763) for the first time reported male sterility in flowering plants. Male sterility is a reproductive deficiency of some plants in which male organs in bisexual flowers become defunct. It relates particularly to nonviable pollen grains that are formed through a chain of vital processes during microsporogenesis. These processes are so delicately balanced under genetic control of many loci that mutation of any one locus may lead to the formation of non-functional microspores, and hence male sterility. In male sterility, pollen grains are non-functional (inactive); but the female gametes (egg cells) are normally functional.

Pollination can be defined as the pre-fertilization event or process where pollen grains from anther are transferred to the stigma of a flower. Pollination is the process that helps to unite the male and female gametes and thus helps in fertilization. It can be broadly classified into two, cross-pollination and self-pollination.

1. Self-Pollination

Self-pollination is the process by transferring the pollen grains directly from anther into the stigma of the same flower. It is called as self-pollination. This form of pollination is common in hermaphrodite or dioecious plants which have both male and female sexual organs on the same flower. In self-pollinating flowers, the anthers and stigma are of similar lengths to facilitate the transfer of pollen. Self – pollination can be further divided into two types:

The emasculation and pollination in any crop depend on its floral structure and biology. Cashew is basically andromonoecious (polygamo monoecious) crop with staminate and hermaphrodite flowers appearing in the same inflorescence or panicle (Rao and Hassan 1957). The hermaphrodite/bisexual/perfect and male flowers occur intermediately in the same panicle in three or four phases viz., Male – Mixed – Male or Mixed – Male – Mixed– Male. Distinct phases depend on genotype and environmental factors. The tree shows considerable variation in the duration of different phases. The hermaphrodite flowers are bigger in size than staminate or male flowers (Damodaran et.al., 1965). Male flower always exceed the bisexual flowers. The flowers produced early in a panicle are mostly male (Rao and Hassan 1957; Damodaran et. al., 1965). Most of the perfect flowers are produced about one month after the first flower occurred in the panicle (Bhat et al., 1998).

Abstract

Pollination studies in apple (Malus x domestica Borkh) were carried out in ten apple cultivars with three pollinizers. Flowering pattern of all cultivars was observed to be same. Flower number per bud ranged from 5.00 to 5.80. All the cultivars started flowering in the second to third week of March. Full bloom was recorded in 4th week of April and flowering period ranged from 16 to 19 days. Maximum percentage of anthesis was observed in the afternoon (12.00 Noon to 2.00 PM). Maximum percentage of anthesis was found in cv. ‘Hardeman’ (33.91%). Dehiscence was recorded to be highest between 10.0 AM to 12.00 Noon. Cultivar ‘Spurtype Red Delicious’ showed maximum percentage of dehiscence (35.59 %). Pollen viability ranged from 85.13 (Spartan) to 89.24 per cent (Jonadel) in two per cent acetocarmine. Best pollen germination was recorded in 15 per cent sucrose solution after 72 hours. Stigma attained receptivity two days prior to anthesis and remained receptive up to two days after anthesis with peak receptivity on the day of anthesis. Under different modes of pollination, the maximum percentage (over all mean) of fruit set was recorded in open pollination (41.98 %) followed by cross pollination (39.12 %), hand self pollination (0.66 %) and selfing by bagging (0.23 %). Maximum average fruit set was obtained when ‘Jonadel’ was used as pollinizer which was closely followed by ‘Ruspippin’. Cultivar ‘Gala’ and pollinizer ‘Spartan’ set fruit on selfing. Cultivar ‘Arlet’ had maximum fruit retention both under open as well as cross pollination in comparison to other cultivars.

Pollination : Transfer of pollen grains from anther to stigma is termed as pollination.

1. Self pollination

When pollen grains from an anther fallen to stigma of same flower or same plant is called self pollination or autogamy. In other word when pollen and pistil are from the same plant, often (but not always) from the same flower. In other word when transfer of pollen grain from anther to stigma of a same flower or same plant is called as self pollination. In which development of seed by self pollination is known as autogamy it is the closest form of breeding. Autogamy leads to homozygosity such species develop homozygous balance and do not exhibit significant inbreeding depression several mechanisms which promote autogamy.

Introduction

Environment pollution is a burning topic of the day. Air, water and soil are being polluted alike. Soil being a “universal sink” bears the greatest burden of environmental pollution. It is getting polluted in a number of ways. There is importance in controlling the soil pollution in order to preserve the soil fertility and increase the productivity. Pollution may be defined as an undesirable change in the physical, chemical and biological characteristics of air, water and soil which affect human life, lives of other useful living plants and animals, industrial progress, living conditions and cultural resources. A pollutant is something which harmfully interferes with health, comfort and environment of the people. Most pollutants are introduced in the environment by waste, accidental discharge, sewage or else. They are by-products or residues from the production of something useful. Due to this our valuable natural resources like air, water and soil are getting polluted. The basis of agriculture is Soil. All crops for human food and animal feed depend upon it. We are losing this important natural resource by the accelerated erosion to some extent. In addition to this the huge quantities of man-made waste products, sludge and other product from new waste treatment plants, and even polluted water are also causing or leading to soil pollution. In order to preserve the fertility and the productivity of the soil.

ABSORPTION (of light): A process by which light is taken-up by another material. Examples include soot consisting of tiny black particles, which absorb all visible light; and nitrogen dioxide, a pollutant mostly from diesel and gasoline engines, that absorbs blue light resulting in air with a brown tint.

Principle

Monomeric acrylamide (which is neurotoxic) is polymerised in the presence of free radicals to form polyacrylamide. The free radicles are provided by ammonium persulphate and stabilised by TEMED (N’N’N’N’-tetramethylethylene-diamine). The chains of polyacrylamide are cross-linked by the addition of methylenebisacrylamide to form a gel whose porosity is determined by the length of chains and the degree of crosslinking. The chain length is proportional to the acrylamide concentration: usually between 3.5 and 20%. Cross-linking bisacrylamide is usually added at a ratio of 2g BIS 38g acrylamide.

Polyacrylamide

Polyacrylamide is a cross-linked polymer of acrylamide. The length of the polymer chains is dictated by the concentration of acrylamide used, which is typically between 3.5 and 20%. Polyacrylamide gels are significantly more annoying to prepare than agarose gels. Because oxygen inhibits the polymer- ization process, they must be poured between glass plates (or cylinders).

Denaturing polyacrylamide/urea gels in TBE buffer

Prepare 20ml of a 5% polyacrylamide gel containing 7M urea by adding:

47.5% acrylamide:

2.5% bis-acrylamide solution 2ml

10M urea 14ml

10X TBE buffer 2ml

10% freshly prepared ammonium persulfate 0.2ml

deionized water 1.8ml

Polyembryony is the occurrence of more than one embryo in a seed. It includes all those instances in which there is a clear indication of the actual occurrence of two or more embryos in a developing ovule (Tisserat et al. 1979). The additional embryos in polyembryony result from the differentiation and development of various maternal and zygotic tissues associated with the ovule of seed. Earlier, polyembryony is said to have an abnormal feature but now it is considered as a desirable character in citrus, mango, jamun, almond, etc. to obtain true to type planting materials. The polyembryony is one of the apomictic processes which have been described to occur in the ovules of angiospermic species (Koltunow, 1993). Polyembryony in horticultural crops occurs as facultative apomixis wherein simultaneous growth of multiple embryos of somatic origin co-exist in the same seed containing sexual embryo resulting from either selfing or cross pollination.

PEG Estimation
Introduction
Polyethylene glycol (PEG) is used as a marker for the estimation of rumen water volume and ruminant digestion studies (Hyden and Ekman, 1953). Long back, Hyden (1955) developed a turbidimetric method which is described below :

Principle
When trichloroacetic acid is added to a solution of PEG, a turbidity is produced, which can be measured photo-electrically. The intensity of the cloud, which consists of finely divided oily droplets, is increased in the presence of barium ions. Protein and sulphate must be completely removed from the sample before the addition of the trichloroacetic acid reagent. Sulphate is precipitated with barium chloride and the proteins are removed essentially according to Samogyi (1945). The method has been developed for determination of PEG in ruminal and abomasal fluids, but it has been tried with other biological materials too.

Introduction

Species of Polygonatum, traditional known for their therapeutic properties for treating different ailments,are fairly well represented in temperate Northern Hemisphere in Asia, America and Europe and find use. In India, P. cirrhifolium, and P. verticillatum are the two important constituting species of Ashtavarga, but have attained a threatened status due to overexploitation. Of the 60 odd known species of the genus, less than 10 have been explored phyto-chemically. Their status varies species wise, at regional and global level.

Abstract

Space, water and labour have become limiting factors in vegetable production. Moreover, production of off-season vegetables during period of demand fetches better returns. Vegetable forcing for year round production has become a necessity in urban and peri-urban olericulture. With use of pesticides, fungicides, weedicides and chemical fertilizers, health hazards have become visible. Being treated as a field crop, vegetables are exposed to vagaries of weather and floods and droughts take heavy toll. Research on protected cultivation in India is limited but for attempts by private seed companies and enterprising farmers in Karnataka, Maharashtra, Haryana and Punjab. More emphasis is given to tomato, cucumber, capsicum, lettuce and beans. Breeding for varieties suited for protected cultivation is a recent innovation in public sector laboratories. Now there are parthenocarpic cucumbers, tomatoes and capsicum especially to suit protected cultivation. The first four vegetable varieties were developed and released by Uttarakhand State Varieties Release Committee for Horticultural Crops during 2011. These varieties namely Pant Polyhouse Hybrid Tomato -1 , Pant Polyhouse Tomato -2 in tomato and Pant Parthenocarpic cucumber -2 and Pant Parthenocarpic Cucumber -3 in cucumber. These varieties were delivered to ployhouse farmers cross the country.

Polymerase Chain Reaction (PCR) is a cutting-edge molecular technique widely utilized for the swift and accurate detection of fish pathogens, especially bacterial infections. By amplifying targeted DNA sequences from minimal sample quantities, PCR enables the identification of pathogens even at very low concentrations.
In India, PCR has become crucial to disease diagnostics in aquaculture, particularly as farming intensifies. It offers early detection of viral, bacterial, fungal, and parasitic infections, including subclinical cases. Given India’s vast range of aquaculture species and environmental conditions—from carp and catfish farming in West Bengal and Andhra Pradesh to shrimp and seabass cultivation in Tamil Nadu and Kerala—PCR diagnostics are increasingly adopted in research institutions, government labs, and commercial hatcheries.
PCR detects the genetic material of pathogens in fish tissues, blood, mucus, or even water and sediment samples. Its high sensitivity and specificity make it ideal for early-stage diagnosis and disease monitoring. Institutions like CIBA, CIFA, TNJFU, and KUFOS have incorporated PCR into their routine health assessments.
The process involves collecting tissue samples (such as gill, liver, or kidney), extracting DNA or RNA, and using pathogen-specific primers in a thermocycler to amplify the target sequence. DNA-based pathogens undergo direct DNA extraction, while RNA viruses require RT-PCR. The results are then analyzed through gel electrophoresis or real-time PCR.

PCR Principles and Procedure

Polymerase chain reaction (PCR) is a technique that allows the generation of large amounts of a single DNA sequence from a mixture of sequences; the fragment generated can be designed to contain specific starting and ending positions based on the needs of the experiment. PCR is used to amplify specific regions of a DNA strand (the DNA target). This can be a single gene, a part of a gene, or a non-coding sequence. Most PCR methods typically amplify DNA fragments of up to 10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.

A basic PCR set up requires several components and reagents. These components include: