eChapters

Introduction

In addition to role of iron oxidation in commercial point of view there are certain implication of iron oxidation on environment. Acid Rock Drainage (ARD) is one of the example of environmental pollution. Coal and various metal ores are enclosed in geological formation of reduced nature. Coal, is often associated with pyrite (FeS₂).When mining activities expose this material to atmospheric oxygen, a combination of auto oxidation and microbial iron and sulfur oxidation produces large amounts of acids. The iron-rich acidic mine drainage kills aquatic life and renders the contaminated stream unsuitable as a water supply or for recreational use.

5.1 The Need

In spite of the considerable experience and quite a few facilities available in the country, the growth of application of isotope techniques is not commensurate with India’s size, its climatic and hydrogeological diversity and the sheer range of its water resources problems.

The situation may not be unique to India and other developing countries. Australia also appears to be facing a similar problem. A recent publication [1] says ‘the limited uptake of the method may be due to a number of factors. Until relatively recently, isotope hydrology has remained a largely academic pursuit, or measurements were the domain of national atomic energy agencies. A large fraction of information lies buried in the grey literature or in arcane technical journals. Interaction with local or regional water resources agencies has been the exception rather than the rule’

India has probably a stronger case for a more effective use of isotope techniques. A large portion of groundwater occurs in hard rocks in India. Normal hydrogeological and geophysical methods cannot fully describe such groundwater regimes to effectively plan their exploitation. It is essential to apply tracer hydrology methods, especially isotope geochemical techniques, for a comprehensive understanding of the systems. The examples of Manikaran geothermal springs, seepage studies in Salal tunnel and in some dams, given in the earlier chapters come under this category. Studies on percolation tanks in basaltic terrain and on recharge processes need isotope support.

It will not be long before we would be planning to exploit very deep ground waters (>500 m), both in the hard rock terrains of peninsular India as well as in the Ganga plains to meet our ever growing need for water. We would need good understanding of the recharge processes to these deep ground waters. Isotopes will have a large role in such investigations.

Arid zone hydrology is yet another area where the potential of isotope techniques has been effectively demonstrated for the characterization of the recharge processes, both palaeo and modern, and including the role of ancient rivers.

Introduction

It has been observed that the surface waters are getting polluted due to improper disposal of domestic and industrial wastewaters. Most of the River basins which are major supply of water for human activities are polluted. Even the groundwater in not spared because of the incessant growth of the population in India. In Maharashtra the number of bore wells has been increased to 5 Lakhs from 1 Lakhs from few decades only. Out of total area of Maharashtra, 82% is covered by the basaltic rocks made up of lava. That’s why the quantity of the groundwater is comparatively less than the Indo-gangetic plane.

Groundwater in the north region of the Tapi River is found to be hard, unfit for the human consumption. The residences of district like Buldhana, Jalgaon and Dhule have to use hard water for consumption. Rampant extraction of the groundwater for agriculture might have caused alternation in chemical qualities of the groundwater. It has been also well documented that in Marathwada region, groundwater content higher concentration of fluoride and total Hardness.

Fluorine the most electro-negative element is of great interest and importance to human health because of its curative and harmful effects. Fluorides are released by various natural sources and anthropogenic activities into the aquatic environment (that is surface as well as groundwater). Fluoride can enter the human body through drinking water, food, toothpaste, mouth rinses and other dental products, drugs and fluoride containing salts and hydrofluoric acid. It may cause Fluorosis – a crippling and painful disease such as dental fluorosis, skeletal fluorosis and non-skeletal fluorosis.

Mango (Mangifera indica L.), the choicest fruit of India, is said to be the king of fruits. Mango belongs to the family Anacardiaceae and genus Mangifera. The fruits are lusciously juicy, with pleasant aroma and sweet taste. In nutritive valve and popularity, no other fruit can match it. It is an outstanding source of vitamin A, a good source of vitamin C, apart from normal minerals and other vitamins. In, India, it occupies the same position as apple does in temperate countries and grapes in certain other regions.

According to de Candolle, mango has been under cultivation in India for over 4000 years. References indicate that Alexander the Great noticed a beautiful mango plantation in the Indus Valley in 327 BC. Early foreign traveller like Huen-tsang (632-645), Ibn-Haukul, (902-968 AD) Ibn- batuta (1325-1349 AD) and Ludovici de Varthema (1503-1508) also praised the mangoes grown in India and mentioned it in their travel notes.

Mango is native to southern Asia, especially eastern India, Burma, and the Andaman Islands. Besides India, mango is cultivated as a commercial crop in many countries, such as, Pakistan, Bangladesh, Myanmar, Sri Lanka, Thailand, Vietnam, Malaysia, the Philippines, Indonesia, the Fiji Island, Tropical Australia, Egypt, Israel, Sudan, Kenya, Uganda, Tanzania, South Africa, the USA (Florida, Hawaii and Puerto Rico), Mexico, Brazil, Haiti and West Indies.

Varietie/Hybrids: Dashehari, Langra, Chausa, Fazli, Malda and Bombay Green are the popular varieties in Northern and Central India. Alphonso, Kesar, Pairi, Rajapuri, Jamadar, and Totapuri are widely grown in Western India. Bangenapalli, Suvarnarekha, Neelum, Totapuri, Malgoa and Alphonso are popularly grown in Southern India and Gulabkhas, Kishen Bhog, Himsagar, Zardal, Fazli and Langra are grown mainly in Eastern part of the country. Mallika, Amrapali, Manjeera, Ratna, Niranjan, Neeleshan, Neelishwari, Neelphonso, Arka Aruna, Arka puneet, Arka Anmol, Sidhu and CISH-M1 are some of the hybird varieties available.

Predatory arthropods provide valuable ecosystem services by suppressing agricultural pests and maintaining ecological balance. Important predators include ladybird beetles, lacewings, predatory bugs, spiders, mites and other groups that consume harmful insects at different developmental stages. Their small body size and ectothermic nature make them sensitive to changing temperature and moisture regimes. Global warming can affect their metabolism, development, body size, fecundity, longevity, dispersal and geographical distribution. Climate change may also disrupt temporal and spatial relationships between predators and their prey. This chapter examines the effects of climatic changes on predatory arthropods and their continuing role in natural and applied biological control.

Industrial revolution took place in England during the period of 1760-1830. Accordingly, the production prior to demand was introduced. Many countries all over the world adopted industrial policies favouring industrial sector to achieve the fast development of the nation. Government of India also contributed its efforts in the same direction and issued the industrial policy of 1948, 1956, 1977 and so on making several financial and non financial facilities available to have substantial growth and development of industries in particular and nation in general.

Under the name of globalization and making the economy internationally competent, India took a revolutionary decision to follow new industrial policy 1991 opening the doors for investment from foreign countries and private sector even. In addition to this economic reforms have been adopted and advocated for the sake of national development. Three ‘D’ programme consisting of devaluation, decontrol and disinvestment, were also put on the national agenda, to achieve the set targets.

INTRODUCTION

Knowledge Resource Centres play a very important role in this fast changing e-environment of publishing. Their role includes identification and selection of information, its organization and management, storage, retrieval and dissemination to right users at the right time at right place at right price and in right format. The dawn of information revolution is considered to be one of the marvels of 21st century. It is well known that paper and printing were originated in China in the 2^nd and 5^th century of Christian era, respectively.(1) There is a “ wordless heaven book”, “ published in oral or paper less form among the Chinese. The word less book is actually an adaptation and extension of “the song of baked dough by Pie-wen Lu” (2) of the 14^th century. The “elite” in every generation have access to the timelessly flexibly text of the heaven-book to interpret the contents and to predict, accordingly, the great events of the world, particularly when it is in chaos. The world refers to, of course, the “middle kingdom,” which is China in Chinese. Although the work is only a legend, the wordless heaven-book can be considered as the pioneer prototype of modern electronic publishing and as the earliest idea of the recently developed hypertext.

Another classic concept of e-publishing can be traced in Gullivers Travels by Jonathan Swift first published in 1726. The work has a passage describing a writing machine developed by the Lagadonians in the Grand Academy of Lagado. The Lagadonian writing machine used the method of a fixed syntactic structure and the filling in of that structure with contents by a random procedure (3).

By Alkaline Lysis Method

Introduction

DNA of prokaryotic cells is relatively simple in comparison to that of eukaryotic cells. In addition to chromosomal DNA, a bacterium may also carry an additional piece of DNA called a plasmid. Bacterial plasmids are double stranded closed circular DNA molecules that range in size from 1 kb to more than 200kb. Plasmids are useful to bacterial cells since they carry genes for antibody resistance, RM system etc that allow bacteria to survive in non-ideal conditions.

Plasmids constructed in the laboratory are used as cloning vehicles. These synthetic plasmids contain only essential features such as origin of replication, antibiotic resistance, multiple cloning sites etc. various copy number of plasmid For e.g. pBR322 20 copy/cell – low copy number, pUC 18, pUC 18- 800 copy/cell – high copy number.

Alkaline lysis, in combination with the detergent sds, has been used for more than 20 years to isolate plasmid DNA from E.coli (Birnboim and Doly 1979). This experiment describes how to extract plasmid from bacteria – which involves growth of bacterial culture, harvesting and lysis of bacteria and isolation of plasmid DNA from the lysate using alkaline lysis method. The DNA is analysed on agarose gel.

Aplasia/Agenesis

Aplasia or agenesis is absence of any organ (Fig. 9.1).

Hypoplasia

Hypoplasia is failure of an organ/tissue to attain its full size (Fig. 9.1).

Etiology

• Congenital anomalies e.g. hypoplasia of kidneys in calves.

• Inadequate innervation.

• Inadequate blood supply.

• Malnutrition.

• Infections e.g. cerebral hypoplasia in bovine viral diarrhoea.

USER EDUCATION

User Education can be defined as a program to guide and to instruct the potential users either individually or collectively. User education is an action taken by the Library staff to train / educate their users so as to make full use of the existing knowledge to develop their community.

ALA Glossary defines user education as it encompasses all types of activities designed to teach users about library services, facilities and organizations library resources and search strategy.

User education can be defined in the context of electronic sources of Information as educating the users how to operate computer and searching Information on Net using various search strategies and to educate them how to use electronic sources of information and various databases.

Users of the library must be convinced that continuing learning is worthwhile and that the process of education does not end with examination. They must have confidence to be learners and to stand of their own, to possess autonomy to select and to direct their own future study experience. User education has been defined by Mews as “instruction given to readers to help them to make best use of library”. User education was not solely to stimulate library use as this but one of the sources of Information. According to Nancy, Fjalbarnt and Lan Malley “ User Education is concerned with the whole Information and communication process and one part if this involves the total interaction of the users the library. This should be a continuous process starting with schools and public libraries and with possibility of extension to academic and specialized libraries. According to Jacques Tacathan, Director of UNESCO general Information Program “User Education is to include any effort or program which will guide and instruct existing and potential user in the recognition and information service and their assessment.

Introduction

Earthquakes are the most destructive among all the natural hazards. Most of the time, they occur without any warning, which makes them most feared and unpredictable natural phenomena. Globally, on an average two earthquakes of magnitude 8 are known to occur every year. Some of the countries like Japan, China and United States have suffered several damaging earthquakes in the past. India has also experienced a number of high intensity earthquakes in the recent past and more than 650 earthquakes of magnitude >5.0 have been reported in India since 1890. Sixty-nine earthquakes in Delaware (U.S.A.) have been documented or suspected since 1871. Refer to Baxter (2000) for more details about the DGS Seismic Network and for documentation of earthquakes. In the 1930s Charles Richter developed a magnitude scale (Richter scale) which was an objective way of discriminating between large and small shocks using the seismic wave amplitude recorded by seismographs (Richter, 1958).

Vegetable crops, known as protective foods, are important for the healthy diet providing vitamins, minerals, fibre, amino acids and other bioactive compounds. Improvement in vegetable crops is inevitable for better yield, pest or disease resistance, morphological characters, nutritive quality, to adapt with environmental stress, climate change, offseason production, satisfying varying consumer preference etc. Vegetable crops comprise of a large number of species and are rich in diversity. Diverse genetic resources are pre-requisite for any crop improvement programme and vegetable improvement is carried out using exotic introductions, indigenous collection and hybrids/varieties by different breeding systems. Characterisation of genotypes is the description of germplasm giving information on heritable characters so that promising one can be utilised in breeding programme which is the best option for higher yield and other qualities. The morphological, physiological and molecular (biochemical and DNA) traits are called qualitative descriptor and yield, pest/disease resistance, stress tolerance etc are quantitative descriptors that are easily influenced by the environment factors. Apart from the higher production and resistance to biotic and abiotic factors, nutritional traits, flavour, processing qualities that increase the food value is also important in improvement programme of fruits as well as vegetables.

Genetic resources of vegetables available at international and national levels are characterised, evaluated and conserved. With the high diversity of vegetable crops, characterisation and evaluation of genetic resources become a stupendous responsibility requiring extensive infrastructure facilities and varying methods of evaluation (Srivastva, 2012). Genetic resources are characterised on highly heritable traits expressed across the environment. Apart from morphological characterisation, biochemical and molecular characterisation is also conducted that are useful for the identification of biotic and abiotic stress parameters, nutritional and processing quality. Genotype characterisation can be done efficiently using potential of biochemical and molecular markers that help to set up the breeding programme.

Introduction

Ligation of DNA fragment is one prime important in genetic engineering because without ligation we could not transfer the interest gene.DNA cloning requires the DNA sequence of interest to be inserted in a vector DNA molecule. For this, both the vector as well as insert DNA is prepare by digestion with compatible restriction enzymes, so that the ends produced during digestion is complementary in both. When setting up ligation, it is important to consider the permutations that can occur and bias the relative concentration of DNA accordingly. Usually a 5- to 10-fold excess of insert over the vector DNA is the norm. This ensures that enough ligated product will be produced in the right orientation.

Introduction

The WTO was established on 1^st January 1995 and is responsible for making and enforcing rules for trade between nations. WTO marks a major change in global trade rules. As an organization, it replaces the General Agreement on Tariffs and Trades (GATT), which had been in existence since 1947. The Eighth Round of Multilateral Trade Negotiations under GATT, which started in Uruguay in 1986, was concluded in 1994, leading to the creation of WTO as the new permanent international trade organization. The role of WTO is much more extensive than that of GATT, which dealt with trade in goods. Apart from goods, the two other broad areas that WTO covers are services and intellectual property, which previously belonged to the domestic domain. Accordingly, WTO administers not only the Multilateral Trade Agreements (MTAs) in goods but also the General Agreement on Trade in Services (GATS) and the Agreement on Trade Related Aspects of Intellectual Property Rights (TRIPS), which came into existence with WTO. All the agreements annexed to the Agreement establishing the WTO were signed as part of a package deal. Member countries did not have the option of choosing some and rejecting others. Another important difference with the erstwhile GATT is that WTO has a stronger compliance mechanism (Chawla, 2007a).

As one of the WTO agreements, TRIPS is binding on all member countries of WTO. TRIPS aims at establishing strong minimum standards for intellectual property rights (IPRs). Apart from patents, intellectual property includes copyrights, trademarks, geographical indications, industrial designs, integrated circuits and trade secrets. The protection of IPRs is binding and legally enforceable. Intellectual property (IP) is a product of the mind. Intellectual property is intangible in contrast to real property (land) or physical property, which one can see, feel and use. With any type of property there are property rights. When IPs are expressed in a tangible form, they can also be protected. Intellectual property rights (IPRs) have been created to protect the right of individuals to enjoy their creations and discoveries. In fact, IPRs can be traced back to the fourteenth century, when European monarchs granted proprietary rights to writers for their literary works.

INTRODUCTION

Information services are keys to the development of agriculture education, research and extension education in India. The services are rendered by the agricultural department, university, college and ICAR institute libraries. In changing scenario the users of libraries access the information themselves from various sources such as computer, internet, television, e-governance, videoconferencing, call centers, mobile phones and other electronic sources from anywhere, at any time as and when required.

Agricultural trends are changing everyday with the development of new research techniques and technologies and in support of this a heterogeneous knowledge is available in the form of various electronic sources. To make the best of emerging technologies in agriculture, it is vital that agricultural students, scientists, research scholars, academicians, extension workers, agriculturalists receive continuous information on education, research, extension work, training and to the cultivators on topics such as pesticide management, use of better seeds, irrigation and use etc. for the development of farm and farming. With the introduction of new technology that affects both their processes and products, libraries become more and more hybrid organizations. Hybrid libraries are mixes of traditional print material like books and magazines, as well as electronic based material such as downloadable audio books, electronic journals, e-books, etc. Because of this shift, libraries face many challenges, the most important of which is the competition for scarce resources. In this competition libraries have to change the allocation of resources from the more traditional processes like circulation to more value added processes like direct self-services to their patrons. The FAO is also a source of knowledge and information, providing behind the scenes assistance to help people help themselves.

Introduction

Cell and tissue culture techniques are becoming increasingly popular as alternative means of plant vegetative propagation. Plant tissue culture involves asexual methods of propagation and its primary goal is crop improvement. The success of many in vitro selection and genetic manipulation techniques in higher plants depends on the success of in vitro plant regeneration. Crop breeding and rDNA technology require the widespread use of reliable true to type propagation and better regeneration methods. Plant regeneration can be grouped in to different categories of enhanced release of axillary bud proliferation, organogenesis and embryogenesis. The oldest practical application of tissue culture techniques is the propagation of disease free plants from meristem tip culture. Meristem tip consisting of dome and one or two subjacent leaf primordial with a size up to 0.25 mm are aseptically cultured in appropriate nutrition media. The resulting plants are disease free because the pathogen is not generally present in the meristematic cells.

The aim of germplasm conservation is to ensure the availability of useful germplasm at any time. Breeding programs rely heavily on locally adapted ancient plant varieties and their wild relatives as sources of germplasm. Further, the continuing search for high yielding varieties of crop plants with resistance to pathogens and pests warrants the availability and maintenance of large collections of germplasm. In species which are propagated through seeds, it is economical to preserve the seeds. These seeds are dried to water content of 5 - 8% and then stored at a temperature of -18° C or lower and low humidity. In case of vegetatively propagated species, preservation of germplasm heavily taxes manpower and land resources. As in vitro techniques are becoming more important in crop improvement through the use of somatic cell genetics, the material produced in vitro will have to be conserved in vitro. The in vitro system is extremely suitable for storage of plant material, since in principle it can be stored on a small scale, disease free and under conditions that limit growth. Germplasm storage in vitro is crucial for the future development and safety of agriculture.

Coastal ecosystems of india

India has a long coastline of 7,517 km that span 13 maritime states and union territories, surrounded by the Indian Ocean, Arabian Sea and Bay of Bengal. It has an Exclusive Economic Zone of 2.02 million square kilometers. The country has a diverse range of habitats: continental area and offshore islands and a variety of coastal ecosystems such as estuaries, lagoons, coral reefs, mangroves, salt marshes, sea grass beds, backwaters, rocky coasts, mudf lats, sand dunes, and coastal beaches. Coasts and islands form 2.8% of India’s geographical area. The coastal ecosystem consists of 43,230 km² of coastal wetlands with 97 major estuaries and 34 major lagoons; 4921 km² of mangroves with 31 mangrove areas, and 3062.97 km² of coral reefs with 5 coral reef areas (MoEFCC, 2018). A network of 14 major, 44 medium and numerous minor rivers together with their tributaries with a total length of over 40,000 km, contribute to the coastal sea directly or indirectly. By area, tidal/mudf lats are predominant and by length, more than half of the Indian coastline is sandy. The states of Gujarat, Tamil Nadu and Andhra Pradesh have extensive coastline (Fig. 1).

Introduction

Western blot analysis can detect one protein in a mixture of any number of proteins while giving you information about the size of the protein. It does not matter whether the protein has been synthesized in vivo or in vitro. This method is, however, dependent on the use of a high-quality antibody directed against a desired protein. So you must be able to produce at least a small portion of the protein from a cloned DNA fragment. You will use this antibody as a probe to detect the protein of interest.

Western blotting tells you how much protein has accumulated in cells. If you are interested in the rate of synthesis of a protein, Radio-Immune Precipitation (RIP) may be the best assay for you. Also, if a protein is degraded quickly, Western blotting won’t detect it well; you’ll need to use (RIP). See the section on RIP for more information, as well as a helpful comparative chart that illustrates the differences between these two techniques.

The performance of plants is affected by the soil in which it grows and climate and micro climate of its surroundings.

Impact of soil

The soil provides anchorage, nutrients and water for plant growth. Soil fertility status, structure and texture influence the plant growth and productivity.

Classification of soil

On the basis of the organic and inorganic contents and particle size and texture, the soils have been classified. The inorganic matter in the soil constitutes of varying amount of sand, silt and clay, on the basis of which the soils are classified as follows.

Sandy soil

These soils have coarse textured and large pore spaces, have poor water holding capacity. Retention of nutrients in this soil is very poor. This type of soil is suitable for precocious crops. Sandy soils initiate rapid root growth, hence good as propagating medium. Since sandy soils has low in nutrient content, supplementation of adequate nutrition is imperative to achieve good plant growth.