eChapters

1. Introduction:- Ensuring Quality and Success in Assisted Reproductive
Technology through Good Laboratory Practices Assisted Reproductive Technology (ART) in animals has become an increasingly vital set of techniques with wide-ranging applications across agriculture, wildlife conservation, and biomedical research. These technologies, encompassing procedures such as in-vitro fertilization (IVF), embryo transfer, and cryopreservation of gametes and embryos, offer solutions to infertility, enable genetic improvement in livestock, and play a crucial role in preserving endangered species. As the application of ART expands throughout the globe, the need for stringent quality control and standardization becomes paramount to ensure the reliability and reproducibility of outcomes. Good Laboratory Practices (GLP) represent a well-established quality system that provides a framework for the organizational processes and conditions under which non-clinical studies are planned, performed, monitored, recorded, reported, and archived. Originally developed to ensure the integrity of safety data for pharmaceuticals and other regulated products, the fundamental principles of GLP are highly relevant to the field of animal ART. This report aims to explore the application of GLP in ART and to thoroughly investigate its contribution to achieving successful outcomes in various ART procedures and across different animal species. By examining the core principles of GLP and their practical implementation in animal ART laboratories, this analysis will highlight the critical role of standardized practices in enhancing the reliability, ethical conduct, and overall success of animal reproductive technologies.
2. Good Laboratory Practices (GLP) in Reference to ART
Good Laboratory Practices are broadly defined as a quality system that governs the organization and conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, reported, and archived. This system is designed to ensure the quality and

There are some weeds which prefer to in crop field and some of them have a distinct association with some crops.

Horticulture is a wide field and includes a great variety and diversity of crops. In general, these crops are classified as follows:

1. Crops grown for the food and/or vitamin and mineral content, for e.g., apple and sweet potato.

2. Crops grown for their beverage properties, for e.g., tea and coffee.

3. Crops grown to beautify the environment, for e.g., the grasses of the well planned lawn.

4. Crops grown to express sentiments, for e.g., cut roses, jasmine.

5. Crops grown for perfume for e.g., rose, jasmine.

6. Crops grown for spices for e.g., pepper, nutmeg.

Botanical Name: Cocos nucifera L. Family: Arecaceae / Palmae Chromosome No: 32 Place of Origin: Malaysia Common Name: Coconut 2.1 Introduction Coconut (Cocos nucifera L.), is widely known as ‘Kalpavriksha’ (i.e., tree of heaven) as each and every part of the palm is useful in one way or the other and is an important perennial oil yielding crop of humid tropics. It provides food security and livelihood opportunities to 20 million people around the globe and 10 million people in India (Chowdappa et al., 2017). Even though India is the largest coconut growing country in the world, its contribution to the export market is not significant. Coconut as an edible oil and food crop, is now slowly transforming into raw material for various pharmaceutical, nutraceutical and cosmetic products. Coconut is rich in fibre, vitamins and minerals. Because of its health benefits beyond its nutritional content, it has been considered as “functional food”. Hence, there exists a huge scope for coconut-based agribusiness in India, which will increase the present 8% level of value addition to 25%. The coconut-based value-added products are desiccated coconut powder, coconut milk, coconut cream, coconut milk powder, coconut flour, coconut water concentrate, coconut jelly (Nata-de-coco), coconut vinegar, virgin coconut oil, coconut chips, coconut pickle, coconut ice cream, neera and neera based value added products such as syrup, squash, honey, jaggery, candy and sugar (Chowdappa et al., 2017).

Management approaches to soil, including problems of soil degradation and low soil fertility, have recently undergone major changes. The earlier concept was to concentrate on achieving high levels of production from the more fertile areas, leaving the marginal lands for extensive use only. Steeply sloping and highly drought-prone areas were mostly left without any cultivation as production from these areas was hardly cost effective. Soil constraints were to be overcome by inputs like improved crop varieties, fertilizers, chemical control of pests and diseases, and the use of irrigation. The approach of use of newly developed high yielding crop varieties, improved agronomic management practices, use of chemical fertilizers, efficient water management and timely plant protection measures has been successful in achieving large increases in crop productivity in recent years. On the other hand, continuous application of fertilizers at higher rates leads to environmental problems. Yield responses to fertilizers have declined because of soil physical degradation and micronutrient deficiencies.

Zoonoses

Zoonoses are those diseases and infections which are transmitted between vertebrate animals and humans under natural conditions. The emergence of a pathogen into a new host species is called disease invasion or “disease emergence”.

Anthrax is a notifiable disease and primarily occurs in herbivores but it is a type of bio-terrorism agent and called as malignant pustule, malignant edema, woolsorters disease, ragpickers disease, maladi charbon, splenic fever etc. Causative agent is Bacillus anthracis contains vegetative spore and lethal dose is 2,500 to 55,000 spores.

Brucellosis is one of the world’s most widespread zoonoses caused by the genus Brucella. In human it is called Malta fever, Undulant fever, Mediterranean fever, Rock Fever of Gibraltar and Gastric Fever while in animals it is called Bang’s disease, Enzootic abortion, Epizootic abortion, Slinking of calves, Ram epididymitis and Contagious abortion etc. it is also called occupational disease.

Leptospirosis is a disease of animal sand humanbeings caused by genus Leptospira and called as Weil’s syndrome, Canicola fever, Canefield fever, 7-day fever, Rat Catcher’s Yellows, Autumn fever, black jaundice). Fletcher’s EMJH media is used to culture the organism.

The transfer foreign gene to the plant cell is a very crucial method to production of transgenic plant. So improvement of plant quality requires to transfer our interest gene or we can say useful for production of our interest product. To achieve genetic transformation in plants, we need the construction of a vector (genetic vehicle) which transports the genes of interest, flanked by the necessary controlling sequences i.e. promoter and terminator, and deliver the genes into the host plant. The two kinds of gene transfer methods in plants are.

1. Vector-mediated or Indirect Gene Transfer

Among the various vectors used in plant transformation, the Ti plasmid of Agrobacterium tumefaciens has been widely used. This bacteria is known as “natural genetic engineer” of plants because these bacteria have natural ability to transfer T-DNA of their plasmids into plant genome upon infection of cells at the wound site and cause an unorganized growth of a cell mass known as crown gall. Ti plasmids are used as gene vectors for delivering useful foreign genes into target plant cells and tissues. The foreign gene is cloned in the T-DNA region of Ti-plasmid in place of unwanted sequences.

To transform plants, leaf discs (in case of dicots) or embryogenic callus (in case of monocots) are collected and infected with Agrobacterium carrying recombinant disarmed Ti-plasmid vector. The infected tissue is then cultured (co-cultivation) on shoot regeneration medium for 2-3 days during which time the transfer of T-DNA along with foreign genes takes place. After this, the transformed tissues (leaf discs/calli) are transferred onto selection cum plant regeneration medium supplemented with usually lethal concentration of an antibiotic to selectively eliminate non-transformed tissues. After 3-5 weeks, the regenerated shoots (from leaf discs) are transferred to root-inducing medium, and after another 3-4 weeks, complete plants are transferred to soil following the hardening (acclimatization) of regenerated plants. The molecular techniques like PCR and southern hybridization are used to detect the presence of foreign genes in the transgenic plants.

India boasts a long-standing heritage of indigenous and ethnoveterinary knowledge in livestock care, developed over generations through careful observation, experience, and adaptation to local conditions. These practices emphasize sustainability and affordability, making them particularly valuable for small-scale and marginal farmers. By relying on readily available, seasonal, and natural resources, they align closely with ecological principles and traditional wisdom.
Feeding methods rooted in indigenous systems often utilize locally sourced tree leaves, grasses, agricultural residues, and customized herbal or homemade concentrates. Fodder options such as neem, babul, ber, subabul, moringa, and arhar leaves are commonly used, appreciated both for their nutritional content and medicinal properties. In several rural communities, animal diets are enhanced with supplements like bran, oilseed cakes, fermented grains, and by-products such as buttermilk residue, all aimed at improving digestion, health, and milk production.
Ethnoveterinary approaches focus on disease prevention and treatment through natural substances. Ingredients like turmeric, garlic, fenugreek, ashwagandha, and shatavari are frequently added to fodder or water to promote immunity, reproductive efficiency, and general vitality. A common post-calving practice involves feeding a mix of jaggery, wheat bran, and ajwain to lactating cows to aid in uterine cleansing and boost milk secretion.
These traditional methods offer a viable alternative to commercial feeds, synthetic additives, and antibiotics. Deeply rooted in regional customs and passed down through generations, they represent a rich repository of community knowledge. Documenting and validating these practices through scientific inquiry and participatory research can reinforce the resilience of rural livestock systems.

1. Introduction

Food is an essential requirement for survival. We work hard to earn money so that we can feed ourselves. However, at the end of the day, many of us are unsure about the quality of food we consume. The allure of money, along with a general disregard for humanity, has resulted in the addition of adulterants to food ranging from simple stones in rice to the more harmful chemicals in food of daily use. Surprisingly, food fraud is not a modern issue. There are several references to fraudulent food behaviour as early as the Romans, but it is usually considered to be the Victorian who places food fraud on the map.

This chapter introduces the close relationship between climate change and forest ecosystems and examines how changing temperature, rainfall, drought, extreme weather, fires, pests, and diseases affect forest health and productivity. It discusses the importance of forests in carbon sequestration, biodiversity conservation, climate regulation, and ecosystem services. The chapter also examines climate-change mitigation through sustainable forest management, afforestation, reforestation, and initiatives such as REDD+. Particular attention is given to the Indian scenario, conservation policies, government initiatives, and challenges associated with forest degradation and climate change

Floral decoration in India has got a historical background spreading over different eras. Admiration of natural beauty, plants and flowers were part of civilization process. Many evidences are there in the paintings, carvings on wall of caves, temples, mosque besides literatural references that flowers were in use in many forms since time immemorial. Every religion, culture, rulers and regimes had great influence on the use of flowers.

At present, flowers are integral part of our daily life. We use flowers in various ways and for many purposes. Loose flowers are used for worship while garlands for decoration purpose during social, institutional, official functions etc. In addition, cutflowers in the form of bouquets are very common in use. Arrangement of flowers in the vase, bowl or any other container is displayed extensively for interior decoration. At present, placing flower vase is very popular in houses, corporate offices, institutes, hotels etc. and is a well established practice.

1. Introduction

Drying is a thermo physical and physicochemical process, which involves removal of excess water from a solid, semi-solid or liquid food material by evaporating it into vapour phase on applying heat, to reduce growth of microorganism or to completely eliminate their growth. Hence, reducing the water activity increases shelf life of food products. Drying medium for carrying evaporated moisture can be air or steam. Benefits of drying include increase in shelf life, easy to transport, handle food material and reduce packaging cost as it reduces bulk weight. 

Introduction
Crop breeding programmes are under increasing pressure for developing cultivars that possess the ability to adapt to diverse environments while maintaining higher quality and quantity, in response to the escalating worldwide demand for food. The primary goal of plant or crop breeding is to attain genetic enhancement, leading to the creation of new varieties that demonstrate higher yield and superior quality. Hence, the central aim of crop breeding initiatives is to develop novel cultivars that demonstrate improved resistance against fungal, bacterial, insect, or viral pathogens, alongside increased tolerance to abiotic stresses such as drought low temperature, salinity, dehydration, and heavy metal toxicity. Additionally these breeding efforts aim to improve quality attributes, including taste, size shape, colour, and cooking convenience (Lenaerts et al., 2019). The ability to anticipate emergent phenotypes resulting from genetic or environmental disruptions necessitates the utilization of methodologies capable of identifying metabolic pathways or canopy structures suitable for change. The insilico tools have the potential to guide breeding endeavours aimed at developing novel germplasm that exhibits robust adaptation to diverse environmental conditions. The precise simulation of organisms in a computational environment is a current and effective approach to enhance prediction abilities. Significant progress has been made in the field of predictive medicine through the implementation of community projects that utilize integrative, multi-scale modeling techniques. However, there are a lot of crop models that are just geographically and temporally restricted, which limits their ability to go beyond the data that is now available and, consequently, leads to insufficient forecasts regarding crop reactions in novel circumstances. The plant community has launched or continued a coordinated endeavour focused on the development of a virtual plant modelling through consolidative and multi-scale mapping, models and simulations (Marshall-Colon et al., 2017).

Introduction
Wide range of industrially important products such as antibiotics, vitamins, enzymes, polymers, amino acids, biofuels are produced by microbes in extremely small quantities. Using microbial strains that generate an elevated amount of the intended product is a prerequisite for effective biotechnological procedures at the industrial scale. But since this isn’t an intrinsic feature of the chosen microorganism, changes to their genetic makeup may help to navigate this. Therefore, in order to overproduce the target metabolite and increase process efficiency, industrially relevant microorganisms are exposed to a range of treatments employing physical, chemical, or genetic methods. Thus, microbial strain improvement is the process of improving the biosynthetic capacities of microorganisms to generate the desired product in greater quantities.
Recycling resources and promoting sustainable development have grown in importance in recent years. Cosmetics, food, fuels, lubricants, plastics, drinks, fibres, and medications are just a few of the goods that are being biosynthesised due to the growing popularity of biological production of commercially significant metabolites (Kaur et al., 2012). The rising bioeconomy’s success hinges on our capacity to produce high-performing strains efficiently and economically. One successful strain engineering strategy is the Design–Build– Test–Learn (DBTL) framework. The strain engineering process must be viewed from the perspective of optimising the entire cycle rather than just increasing productivity at each stage, if strain development time and cost is to drastically cut down. We suggest a strategy that incorporates all four phases of the DBTL cycle and leverages the latest developments in phenotyping techniques, high-throughput genome engineering, computational design, and machine learning tools to forecast strain scale-up efficiency. By substituting sustainable alternatives made from renewable feedstock for petroleum-based products and ingredients derived from animals or wild-harvested plants, microbial biomanufacturing of chemicals, materials and biomolecules promises to lower greenhouse gas emissions, enhance land use and promote sustainability. But doing so necessitates creating reliable and effective industrial strains that can generate a variety of goods at reasonable costs. Enhancing the effectiveness of the strain engineering process is necessary to lower development cost, time in order to take advantage of market potential in all bioeconomy industries.
Over the past few decades, technical advancements in genome engineering have significantly boosted our capacity to engineer biological systems. The strain engineering process has been significantly sped and enhanced by synthetic biology methods like as genome editing using CRISPR (clustered

Dyes are one of the most important by products of the plants, as they are related with cultural practices, rituals, arts and crafts, fabrics and to satisfy personal embodiment. However, dye yielding plants have not received significant attention (Grover and Patni, 2011). Dye is a substance that has affinity to the substrate to which it is being applied such as textile fibers, foodstuffs and powder. They are organic coloured substances which absorb light in the visible part of the spectrum and have the property of colouring the substrate they are applied to.
 
Colourants which are obtained from plants and natural sources are known as natural dyes. Natural dyes have been traditionally used in most of the countries. These dyes were obtained from roots, leaves and bark (Akhtar et al., 2012). Natural dyes find use in the colouring of textiles, drugs, cosmetics, etc. Owing to their non-toxic effects, they are also used for colouring various food products. In India, there are more than 450 plants that can yield dyes (Siva, 2007).

“Small is beautiful”, today we know that small is not only beautiful but also powerful. With the range of applications that nanoparticles find in varied fields of engineering and science, nanoparticles seem a promising option when compared to the conventional materials used. Nanoparticles are particles that have at least one dimension in less than 100nm range. They have a high surface to volume ratio and thus mass transfer and heat transfer properties are better than bulk materials. Recently; core/shell nanoparticles are finding widespread application. There is a class of core/shell nanoparticle that has its entire constituent in the nanometer range. Core/shell nanoparticles are nanostructures that have core made of a material coated with another material. They are in the size range of 20nm-200nm. Also, composite structures with these core/shell particles embedded in a matrix material are in use. The necessity to shift to core/shell nanoparticles is the improvement in the properties. Taking into consideration the size of the nanoparticles, the shell material can be chosen such that the agglomeration of particle can be prevented. This implies that the monodispersity of the particles can be improved. The core/shell structure enhances the thermal and chemical stability of the nanoparticles, improves solubility, makes them less cytotoxic and allows conjugation of other molecules to these particles. The shell can also prevent the oxidation of the core material. “When a core nanoparticle is coated with a polymeric layer or an inorganic layer like silicabecausethe polymeric orinorganiclayerwould endow the hybrid structure with an additional function/property on top of the function/property of the core hence synergistically emerged functions can be envisioned”.

Banana is one of the oldest fruits and second largest growing fruit crop in the world. It is also known as the Adam’s Fig and Apple of Paradise. Unlike other fruits banana is an herbaceous fruit plant. Banana breeding started in Trinidad, West Indies in 1922 and in Jamaica in 1924 (Shepherd, 1994). The driving force for this breeding programme was to develop improved Fusarium wilt (Fusarium oxysporum F. sp. Cubense) resistant banana for export trade (Buddenhagen, 1990). In 1960 both the programmes were combined under the Jamaica Banana Board. United Fruit Company also started a small breeding programme in Panama in 1920s. In India hybridization work was started at Central Banana Research Station, Aduthurai, Tamil Nadu in 1949. Important banana growing states are Maharashtra, Karnataka, Kerala, Tamil Nadu, Andhra Pradesh, Orissa, Bihar, West Bengal and Assam. Now a days in some districts of Uttar Pradesh, Harichal cultivar of banana is cultivated at commercial scale. In South India banana is extensively used in all auspicious occasions such as wedding, festival and worship. Banana is a good table fruit, besides, the cultivar Nendran is used for cooking, it is also used for preparation of halwa, sweet and chips etc. Main bud or heart of banana bunches is also used as vegetable in India, Thailand, Philippines, Malaysia and Indonesia. Musa textiles is known for strong fibre quality.

INTRODUCTION

Digital resources are growing at an exponential rate and uncountable stacks of resources are available on the web. Semantic based search engines and meta-search engines stimulate resource discovery on digital collections1. During recent years an increasing quantity and variety of digital material has been created or held by libraries, and consequently many standards have been developed to encode different catego-ries of metadata for specific object types. Metadata today is an essential phenomenon for electronic cataloguing, federated searching, and open URLs. Selecting metadata is perceived to be essential for the librarians in pursuing long-term management and preservation of digital objects in digital library. Metadata is also important for digital archivists, database developers, resource authors, web-page designers, aggregators, system designers, as well as seekers of electronic information. In fact, metadata is inevitable for digital resource management and for discovering information from a large distributed environment. Metadata is expected to improve matching by standardizing the structure2 and content of indexing or cataloguing information. The Metadata Encoding and Transmission Standards (METS) were developed to deal with diverse metadata is-sues. It is an XML Schema designed as an overall framework3 within which all the metadata associ-ated with a single digital object can be stored or referred to. It enables effective management of digital objects within the repository; acts as a standard for transferring metadata within repositor-ies, facilitates access and navigation by the researcher, and links the digital object and its meta-data inextricably together.

In recent years dietary supplements have gained attractiveness within fitness and wellness circles. The other commonly used terms areNutraceuticals, dietary supplements or wellness-focussed foods. Being highly nutritious they are associated with a number of potential therapeutic effects. Nutrient-rich ingredients like fruits, vegetables, nuts, seeds, and grains are often considered dietary supplements as well. Oats fibre called, beta glucan, which has been shown to reduce inflammation, enhance immune function, and perk up heart health.

1. Conventional and Non-conventional Dietary Supplements

Nutrient-rich foods like fruits, vegetables, and legumes are often considered dietary supplements. Meanwhile, modified foods have been fortified with micro and macro nutrients to boost health benefits of foods.

• In most insects, life begins as an independent egg (ovipary).

• Majority of insects are oviparous. Egg stage is inconspicuous, inexpensive and inactive. Yolk contained in the egg supports the embryonic development.

• Eggs are laid under conditions where the food is available for feeding of the future young ones.

• Production of eggs by the female’s body is called oogenesis and the egg laying process is known as oviposition.

• The outer protective shell of the egg is called chorion. Near the anterior end of the egg, there is a small opening called micropyle which allows the sperm entry for fertilization. Chorion may have a variety of textures.

• Size and shape of the insect eggs vary widely.