eChapters

Introduction
The eco-friendly and commercially sustainable production has been a crucial aspect of modern commercial development. The ecologically sustainable microbial production of value-added products has seen rapid growth across multiple sectors, such as food, pharmaceuticals, and energy, resulting in notable economic benefits and social advancements on a global scale (Shi et al., 2022). The development of “omics” technologies has aided the improvement of microbial production processes through diverse omics procedures, such as genomics, transcriptomics, metabolomics, and systems biology (Amer and Baidoo, 2021). These omics approaches give important understandings into the widespread metabolic variations, thereby identifying the factors that affect microbial production efficiency and improving the understanding of targeted optimization strategies. The integration of omics approaches is considered essential for any strategies that aim to be industrially sustainable. Industrial activities depend on the cultivation and accessibility of significantly competent microbial species to optimize bioproduct yields (Keasling, 2012). Recent progress in the industrial generation of value-added products heavily depends on strategies rooted in omics. Typically, the industry can achieve cost-effective production of limited quantities of biopharmaceuticals (Harris et al., 2015). Nevertheless, to sustainably scale up production capacities, economically feasible alternatives must be planned, standardised, and developed. Over the past few decades, microbes with enhanced abilities for superior product generation have been identified (Babar et al., 2018; Kim et al., 2015). Latest improvements in metabolic engineering have aided the manipulation of various genetic components, including coding and regulatory regions, to maximize commercial benefits (Babar et al., 2018). Genomic methodologies aim to investigate the variations among individuals at both the germline and somatic levels by sequencing specific genomes. The transition from DNA microarray technology to DNA sequencing has enabled the thorough sequencing of entire genomes, providing a detailed characterization of an organism’s genomic landscape (Dai and Shen, 2022) whereas, transcriptomics involves the analysis of mRNA, microRNAs, long non-coding RNAs, and circular RNAs. The methods used in transcriptomics are designed to identify and enumerate RNA molecules transcribed from a specific genome at a particular time. Proteomics on the other hand focuses on understanding the functional significance of all proteins expressed within a cell, tissue, or organism by examining the flow of information via protein signalling and metabolomics contributed a vital part in the development of bioprocesses by providing a comprehensive overview of metabolic activities (Horgan and Kenny, 2011; Petricoin et al., 2002). Synthetic biology contributes a vital part in the production of high-performance strains for microbial manufacturing. A variety of microorganisms including bacteria, fungi, and microalgae can be employed in microbial production. However, synthetic biology methods primarily emphasis on the balanced blueprint of biosynthetic metabolisms for target outcome, as well as the creation and standardisation of these pathways and the heterologous production of the biosynthetic components (Wan et al., 2023). These approaches include the optimisation of fermentation process, optimisation of medium composition, scale up of fermentation, optimization of cell growth for manufacture of desired product, and strategies to decrease feedback inhibition and product toxicity (Figure 3.1) (Wan et al., 2023; Zhang et al., 2022).

Abstract

Development of reliable automated techniques for the identification and annotation of exon and intron regions in a gene has been an important area of research in the field of computational biology. Several classical approaches like probabilistic model, artificial intelligence and digital signal processing are already in use to cater to this challenging task. In this work, we propose a Support Vector Machine based kernel learning approach for detecting splice site patterns and their conservation across the species. Electron-Ion Interaction Potential (EIIP) values of nucleotides were used to map DNA character sequences to corresponding numeric sequences. Radial Basis Function (RBF) SVM kernel was trained with cross validation using these EIIP numeric sequences. This was then tested on test gene datasets of multiple species for detection of splice site pattern. Receiver Operating Characteristic (ROC) curves and various other statistical measures have been utilized for displaying the performance of the classifier. Results indicate an inter-species splice site conversion profile.

SUMMARY

Abiotic stress can be reduced by choosing the most appropriate pulse species and adjusting agronomy (sowing time, plant density, soil management) to ensure sensitive crop stages occur at the most favourable time in the season. For example choosing the optimum sowing time, species and cultivars with appropriate phenology can reduce the effect of frost and drought in pulse crops in dry land environments, which termed stress.

             Most appropriate approach of dealing with stresses caused by extremes in the abiotic environment is to develop cultivars resistant to specific stresses. However, breeding and selection for resistance to these stresses is often considerable difficult because of the unpredictability of climatic conditions. Drought and heat escape through earliness in flowering and maturity is probably the characteristic most widely used by breeders for pulses and other crops to escape drought especially in low rainfall, terminal drought environment. Lack of simple and accurate screening procedures to screen parental genotypes and breeding population for various abiotic stresses is the major bottlenecks in the development of stress tolerant cool season pulse crops.

Botanical Name: Theobroma cacao L.

Family: Sterculiaceae

Chromosome No: 20

Place of Origin: Tropical South America

Common Name: Cacao, cacao bean, cocoa, cocoa bean

6.1 Introduction

Cocoa (Theobroma cacao L.) is a perennial plant and is used as beverage crop. The generic name Theobroma means the “Food of the God”. The word cacao refers to the tree, while the word cocoa refers to the drink made from its seeds. The roasted product of the dried beans is called as Cacao nibs that are used for the manufacture of various products. When cacao nibs are ground, the resulting product is called chocolate liquor or mass, which contains around 55% fat. The fat that is pressed from chocolate liquor is termed as cacao butter. It is mainly used for the manufacture of chocolates, in pharmaceutical preparations and soap making. After pressing out the fat the resultant product with reduced fat content is called cacao powder. For preparation of chocolates cacao powder is mixed with cacao butter and sugar with a definite ratio. Milk chocolates are prepared by adding milk to the above mixture. The cocoa powder contains 22- 25% fat, 20.4% protein and 35% carbohydrate with caloric value of 452 cal. Rind or shell of pod is used as a cattle feed.

For effective and economic control of pests, besides selection of appropriate plant protection chemicals, application of the chemicals at the most vulnerable growth stage of the pests and use of chemicals at the correct dosages, selection of proper plant protection appliances to carry out the operation is also of vital importance so as to ensure uniform application of the chemicals.
 
Plant protection chemicals are mostly used as sprays and dusts using sprayers and dusters respectively. However, in most of the cases spraying is preferred to dusting. Different types of sprayers and dusters are being used for these operations and in both, there are manually operated as well as power operated equipments and in sprayers there are both hydraulic and pneumatic types of sprayers.

The nature of the pesticide formulations to be used and the purpose for which they are used also determine the type of equipment to be used. Dusters are used for dusting insecticidal dust formulations; sprayers are used for spraying insecticidal liquid formulations; granular applicators are used to apply granular formulations; fumigators are used to apply fumigants to fumigate godowns, warehouses, rat holes etc.; soil injectors are used to inject pesticides at a particular depth of soil under pressure; flame throwers are used to throw flames at specified targets.

Abstract

Increased human activities such as agriculture, mining, and manufacturing have resulted in rapid increase of green house gases (GHGs) in the atmosphere. Atmospheric accumulation of the GHGs results into global warming and climate variability or change. Climate change due to enhanced greenhouse effect arising as a result of human activity is considered a major global environmental threat to mankind today. Perhaps nowhere in the globe are people more vulnerable to climate change than in Sub-Saharan Africa (SSA). Most livelihoods in SSA are climate-sensitive due to over dependency on land and other natural resources and are thus perched on the brink of disaster due to climate change.

Climate change is now undoubtedly affecting millions of people across Africa and it actually threatens to wipe-out the past and current global efforts made to tackle poverty unless urgent and robust decisions and actions are taken. This is also a wake-up call for the world to adopt alternative development models that would ensure massive cuts in GHG emissions to avoid a potentially irreversible change. Scientists have warned that unless the global community, especially the developed countries, take genuine steps now to reduce their emissions; many more millions of people in the developing world will be negatively impacted by climate change.

Role of Male and Female Extension Workers

Extension Workers include all persons working in the field for improving the qulaity of life of people. In our field, Gomitras, Livestock Inspector, VAS, BVO, etc are all extension workers because some way or other they are involved in promotion of livestock activites in the field. The role of extension worker working with rural people is to bring necessary change inindividual and collective behaviour and motivating them. The role of male and female extension worker is not very distinct. However, in some backward communities, the role of the female extension worker to motivate women, is found to be very encouraging. Here, we will discuss the important roles of both male and female Extension workers.

An extension worker should arouse farmers to recognize and take interest in their problems, to overcome these problems, to teach them how to do so, to persuade them to act on his teaching, so that they ultimately achieve a sense of satisfaction and pride in their achievements.

Botanical Name: Anacardium occidentale L.

Family: Anacardiaceae

Chromosome No: 42

Place of Origin: Brazil

Common Name: Cashew, cashew tree, caju

7.1 Introduction

Cashewnut (Anacardium occidentale L.) is an important tropical tree crop which leads the edible nuts in international trade with 29% market share only after hazelnuts (35%) and followed by walnuts (21%) and almonds (16%). It is one of the major exports earning crops which accounts nearly Rs. 2515 crores contributing about 1.5% of the total Indian exports. It was only during the past two decades it gained the status of horticulture crop. India is the world’s largest producer of cashewnut. The cashew cultivation area in India during 2018-19 was 10.89 lakh hectares with an annual production of 743 thousand metric tons of fruits. Maharashtra is a much-advanced state for horticulture which occupies large area (1.9 lakh hectares) under this crop (Directorate of Cashew & Cocoa Development, Cochin, Kerala). Cashew exports amounted to over 566 million U.S. dollars from India in fiscal year 2020. This included cashew kernels and cashew nut shell liquid. India’s main export markets for this commodity included South Korea, China, Spain, United States and Belgium (Department of Agriculture, Cooperation & Farmers Welfare).

SUMMARY

Multiplicity of cropping systems has been one of the main features of Indian agriculture and it is attributed to rain-fed agriculture and prevailing socio-economic situations of farming community. It has been estimated that more than 250 double cropping systems are followed throughout the country and based on rationale of spread of crops in each district in the country, 30 important cropping systems have been identified. Cropping systems of a region are decided by and large, by a number of soil and climatic parameters which deter mine overall agro ecological setting for nourishment and appropriateness of a crop or set of crops for cultivation. Much of the Madhya Pradesh area also coincides with the rainfed semi-arid agro-ecological region and sub-regions which are upland, forested with low agricultural productivity and predominately tribal population. It would appear that over the centuries – including in recent history tribals have been inexorably moved off the better lands, into areas which from an agricultural perspective have low productive capacity and little potential for growth. Low agricultural productivity is one of the main causes of persistently high poverty rates in the state and the poor masses not only depends upon the farming system but also chosen different alternatives for their sustanability.

I. INTRODUCTION

Over the last few decades, protection of intellectual property rights (IPRs) has become a global issue. The existing international intellectual property rights regime has been governed by the Trade- Related Aspects of Intellectual Property Rights (TRIPS) Agreement.1 Inspite of maturity of intellectual property laws in post TRIPS era it still desiderate to established coherence with human rights laws particularly right to health and maintain a distance with the socio-economic dimension of intellectual property protection. On the other hand the international human rights instruments from the very beginning appear to be considerable to make certain links with intellectual property rights.2 The issue that is now debated that the adoption of the TRIPS Agreement provisions have serious impact on the realization of certain basic human rights such as the right to health because of non availability and affordability of much needed medicines for the poorer populations of the world particularly in majority of developing countries. Meanwhile TRIPS flexibilities as confirmed by the Doha Declaration on Public Health were adopted in 2001 by the global community to address medicines as public health issue.3 Keeping in view the above developments the problem of right to health and the relationship between medical patents and access to medicine in the case of the HIV/ AIDS and T.B epidemics is complex challenge in the context of the 2030 Agenda for Sustainable Development Goals in 2015 adopted by Member States of the United Nations in Sep, 2015. One of the aims of the agenda is to ensure healthy lives and promote the well-being of people of ages.4 In such a situation it is high time to revisit the existing intellectual property regime in the broader context to protect everyone who is likely to be negatively affected by intellectual property standards imposed by developed nations. Under this backdrop the present paper wishes to examine the various kinds of potential impacts of intellectual property regime on the realization of access to medicine as part of human right to health as universal entitlements amongst the most disadvantaged and marginalized peoples and communities in the broader context of sustainable development goals.

Introduction

‘Cole crops’ is a general term used to describe several vegetables in the Brassicaceae family. It is an important and highly diversified group of vegetable crops grown worldwide that belongs to Brassica oleracea (Monteiro and Lunn, 1998) and includes particularly six Brassica crops (cabbage, cauliflower, Brussels sprouts, broccoli, kale and knol kohl). All of these crops can trace their history to a common ancestry of wild cabbage originating in the Mediterranean and Asia Minor regions. Cole crops are one of the oldest cultivated plant groups in the world. They are cultivated in Europe since very ancient time from where they have spread to other parts of the world (Nieuwhof, 1969). All the cole crops (except few cauliflower, broccoli and cabbage types) require chilling temperatures for about two months to transform from vegetative phase into reproductive phase. However, the period of chilling requirement differs according to the kind of crop and varieties. Cole crops are also commonly called as European or biennial vegetables due to their cool temperatures requirements and two season requirement to complete their life cycle.

Major Resources of Cole Crop Germplasm

Germplasm is any form of the hereditary material from an organism. Genetic resources encompass all the various forms of germplasm that are available for collection, storage and use. The genetic diversity of crops, represented by traditional local cultivars and wild relatives has been disappearing rapidly during recent decades. Initial concern was noted by H.V. Harlan in the 1930s (Harlan and Martini, 1936) but by the 1960s, modern plant breeding and land-use changes accelerated loss and stimulated the realization that genetic resources in regions of diversity were being lost at an alarming rate. To conserve the genetic variation within the cole group, a comprehensive base collection of the cultivated forms of Brassica oleracea has been established at the vegetable gene bank of Horticulture Research International (HRI), Wellesbourne, (U.K.). Gene banks have also been established at Instiuut de veredeling van Tuinbouwgewssen, Wageningen, the Netherlands and the Instituto del Germoplasm, Bari, Italy (Van der Meer et al., 1984) for conservation of genetic resources. The International Board for Plant Genetic Resources (IBPGR) has recognized HRI as an international centre for conservation of Brassica oleracea (Innes, 1975). Large number of collections of cole crops is also available with the United States Department of Agriculture, Plant Introduction Service. In India, National Bureau of Plant Genetic Resources (NBPGR), New Delhi has been assigned the duty of conserving the germplasm of all crops, including vegetables. Germplasm of all the cole crops is being maintained at the Indian Agricultural Research Institute, New Delhi (tropical types) and its regional station at Katrain (temperate types). Other major institutes maintaining germplasm of cole crops include Punjab Agriculture University, Ludhiana, G. B. Pant University of Agriculture & Technology, Pantnagar, Indian Institute of Vegetable Research, Varanasi, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan and SKUAS&T, Srinagar.

1. Introduction

Heat transfer from a hot surface is a general method or technique of heating liquids and slurry type of food materials. In such cases the heat can be generated either directly by a heating element or indirectly from a hot medium like steam or hot water via a heat exchanger (e.g. shell and tube, plate). Therefore, for the heat transfer during heating by these techniques a temperature gradient will be required, which usually causes the fouling on the surface of the heating element or heat exchanger. Afterwards the fouling not only reduces the heat transfer rate but also results in quality deterioration within the product which is being processed. 

6.1 Introduction 
Lignocellulosic Biomass (LCB) generated from the global consumption of fruit and vegetables rapidly generates approximately 140 billion metric tons of agricultural waste. Environmental pollution associated with these wastes, like land pollution, and increased greenhouse gas emissions, can be reduced through the bioconversion of wastes (renewable biomass resources) into value-added products in biorefineries (Babu et al., 2022). These value-added products synthesized for utilization in industries such as biofuels, biomaterials, or biochemicals are effective, reuseable, and enhance sustainability. These bioproducts are recuperated into useful biomolecules and oils for the food industries and dyes for the textile industries. The sustainability of these products can be acquired efficiently through costeffective pretreatments and bioprocess optimization methods (Haldar et al., 2020). The initial stage of value product chain development is the process modelling and optimizing, which, due to its complex nature and large scale, faces challenges in raw material availability, cost competitiveness, upscaling, and lack of technological maturity (Gerassimidou et al., 2021). Optimization of production processes like fermentation and chemical synthesis for manufacturing value-added products has become a focal point in research enhancement of biorefineries. There has been a revolution in the technology used in process designing and manufacturing practices in biorefineries. Advanced Artificial Intelligence (AI)-based approaches like Genetic Algorithm (GA), Fuzzy Inference System (FIS), and Artificial Neural Network (ANN) are being utilized along with conventional tools to optimize variables interacting in production processes. These techniques increase overall efficiency and enhance prediction accuracy and output of value-added products (Banerjee et al., 2025). Optimization of the bio processes enables higher productivity in parallel with lower environmental burdens. AI-based approaches integrated with conventional approaches need a large amount of experimental historical data to be successfully carried out (Arias et al., 2023). Advancement of biorefineries due to the rapid demand of value-added products as well as a solution to the greenhouse emissions, the integrated application of AI for process analysis needs to be understood. This chapter is a contribution towards upscaling the current knowledge base related to different approaches for the enhancement of production for value-added products. The chapter discusses the conventional techniques for value-added production and various optimization models. There is further discussion of advancements in the traditional approach using AI-based approaches and their features and benefits in biorefinery. Apart from different AI-based models, AI integrated with life cycle analysis for sustainability is also briefed. Finally, the chapter addresses current limitations in the field and outlines potential future directions for research and development.

Abstract

Insect pests, fungi, bacteria and other injuries are found in all types of environment and affect the growth, production and survival of both plants and animal species in one way or other. Similarly a large number of insects belonging to different orders, families and genera feed on pods and seeds in natural/ during storage and cause injuries. Regeneration failure in several broad leaved and conifers in India and other countries by heavy insect attack in the natural stands at the time of inflorescence and seed formation and on the ground after seed shedding causes heavy loss to number of afforestation programmes. Several methods like chemical, cultural, natural and biological are available for reduction in loss caused to seeds by insects. Generally, earlier the control measure, the smaller the destruction. Forest seeds can be stored safely by taking some precautions before their final storage, viz. inspection of the seeds for their moisture content, visual examination of cleaning method, scanning through soft X-ray technique, thermotherapy or treatments with chemicals, etc. The present chapter has covered review regarding damage caused to seeds by different insect pests and their management options.

Abstract

Political participation of women, on equal terms with men, at all levels of decision-making is essential to achieve the principles of equality, sustainable development, peace and prosperity. Participation is a development approach, which comprises the involvement of disadvantage segments of population in the design and implementation of policies concerning their wellbeing. To achieve gender equality by 2030 requires urgent action to eliminate the many root causes of discrimination that still curtail women’s rights in private and public spheres. Discriminatory laws need to be changed and legislation adopted to proactively advance equality. 49 countries still lack laws protecting women from domestic violence, while 39 bar equal inheritance rights for daughters and sons. Based on data from 87 countries, 1 in 5 women and girls under the age of 50 will have experienced physical and/or sexual violence by an intimate partner within the last 12 months. International efforts through UN are being encouraged throughout the world but are constrained by lack of national policy, cultural factors, social factors, religious factors, economical factors and role of gender base patriarchy. Secular and sustainable democracy prevails only when women experience benefits equally with their male counterparts. Creating adequate space in politics for women participation is essential to sustain real democracy and oblige mothers, wives, daughters and sisters and Gender Responsive Governance is the best strategy for this which embodies measures, attitudes and practices of different stakeholders, both men and women, at different levels of governance with a clear purpose to impact issues that foster women’s empowerment and promote gender equity and social justice. It is a process that promotes and sustains the ability of women to fully participate in the governance and development process, enhances their ability to raise critical questions about inequality and violence against them without fear and pressure.

Carrot, radish, turnip and beet root are major root crops grown in India. In addition, root crops like rutabaga, parsnip, parsley, chervil and celeriac are grown in a limited scale in different parts of the world. There are several other roots and modified underground plant parts that grow in forests and consumed by tribes, but they are not commercially cultivated. Carrot, radish, turnip and beet root are generally consumed as cooked vegetables or fresh salads or as pickled vegetables.

5.1. Carrot

Carrots belong to the Umbelliferae family, named after the umbrella-like f lower clusters that plants in this family produce. As such, carrots are related to parsnips, fennel, parsley, anise, caraway, cumin and dill. Carrot, Daucus carota L. is believed have been primarily originated in Afghanistan, South western Asia and Mediterranean region. Daucus carota ssp. Carota is the most common wild form in Europe and South West Asia, and the present day cultivated carrots have most probably originated from this sub-species.

Haryana, Andhra Pradesh, Punjab and Tamil Nadu, Karnataka, Uttar Pradesh and Assam are the major producers of carrot in India.

Cultivars/Varieties: Varieties with long, orange coloured and smooth roots are preferred in India. Both indigenous and exotic varieties having different root length, shape and colour are grown. Temperate or European type varieties are Nantes, Half long, Early Nantes, Chantenay, Chaman, Pusa Yamadagni and Ooty-1, which require low temperature of 4-8°C for flowering. Tropical or Asiatic type varieties do not require low temperature for flowering and can be grown in plains also. The varieties belonging to this group are Pusa Kesar, Pusa Meghali and Hisar Gairic. Some varieties like Gold King, Indian Kuroda and Super Kuroda are also marketed by private seed companies.

Botanical Name: Camellia sinensis L.

Family: Theaceae

Chromosome No: 30

Place of Origin: China

Common Name: Tea

9.1 Introduction

Tea (Camellia sinensis L.) of family Camelliaceae or Theaceae is the oldest and second most widely consumed beverage in the world following water. Tea is an evergreen plant that mainly grows in tropical and subtropical climates. India is world’s 2nd largest tea producer after China with production of 1.25 million tonnes in 2020. In India it is grown in an area of 6 lakh hectares, contributing one fourth of world total tea production annually. Interestingly, India is also the world’s largest consumer of the beverage and using nearly 30 percent of the world’s tea output. India stands third in terms of tea export after Kenya (including neighbouring African countries) and China. The total tea export was US$ 501.16 million in April 2020 to November 2020 and for November 2020 it was US$ 71.61 million.

1. Introduction

Thermal processing treatments are the conventional methods that have been broadly employed in the food industry to guarantee the microbiological safety of food materials at a minimal cost. However, these thermal treatments cause undesirable changes in flavor, nutritive value, texture, or color of the finished product that impact food quality (Pan et al., 2017). Growing the modern consumer’s demand towards the minimally processed and “fresh-like” taste foods has challenged the food industry to develop novel non-thermal preservation techniques as an alternative to thermal processing. 

Introduction

The rumen ecosystem is essentially a fermentation system that houses a vast array of different microbes. Ruminants typically browse low energy forages, such as grasses or other plant material, rich in fibre but not easily accessible to the ruminant without rumen fermentation. These forages are literally food for the microbes present in the rumen first and then, in turn, the products of these microbes are the feed for the ruminant. Microbes break down forage in the anaerobic rumen environment and the products of microbial fermentation provide the ruminant with metabolisable compounds, such as short chain fatty acids, while the microbes themselves account for around 90% of the amino acids entering the lower intestine. There are many significant challenges to investigate rumen ecology.

Aquaculture is the culture of aquatic organisms per unit water area per unit time for maximum profit. The economically important finfishes and shellfishes, whose body physiology is adjustable to varied environmental conditions, are considered as aquaculture candidate species. So, an overall knowledge on the physiology of such species is required to improve the culture practices.

1.1. Finfish

A fish may be defined as a vertebrate, adapted for a purely aquatic life, propelling and balancing itself by means of fins, and obtaining oxygen  (O2) from the water for survival and life processes. As the fishes have fins, they are also known as finfishes. Fish describes a life from rather than a taxonomic group. They share certain features with other vertebrates. These are gill slits, a notochord, a dorsal hollow nerve cord and tail. In number of individuals, and may be also in number of species, fishes are presently superior to other vertebrates, including mammals. There are 20,000 different species of fishes. Fishes form largest group of vertebrates, Pisces. The various types of fishes differ so much in shape, color and size that it is difficult to believe they all belong to the same group of animals. Fish in general are cold-blooded and adjusted either to freshwater, brackishwater or marine environment.