eChapters

ABSTRACT

Dysoxylum binectariferum Hook.f. (Meliaceae) is phylogenetically related to the Ayurvedic plant D. malabaricum. D. binectariferum has been identified as an enriched source of rohitukine, an alkaloid. Two synthetic flavones, flavopiridol (Alvocidib; L868275; HMR-1275; NSC 649890 of Sanofi-Aventis + NCI) and P-276-00 (Piramal) are advanced in clinical trials for the treatment of cáncer. These two molecules are considered as potent Cdk inhibitors. Cdk has been discovered as a key regulator of cell cycle. Therefore, Cdk inhibitors or modulators are of great interest to explore as novel therapeutic agents. These two molecules can be considered as rohitukine inspired drugs since their stuructural novelity originated from a compaign to develop a route of total synthesis of rohitukine. Recently a semisynthetic derivative of rohitukine; IIIM-290 has been emerged as potential preclinical cyclin-dependent kinase (Cdk) inhibitor. Moreover, phytochemical investigation of D. binectariferum yielded dysoline a new region-isomer of rohitukine. Dysoline seems to be a potent inflammatory and cytotoxic compound. Similarly, camptothecine and 9-methoxycamptothecine were isolated from Dysoxylum binectariferum for the first time by bioassay-guided fractionation. Present chapter is reviewing some original work which were carried out at CSIR-Indian Institute of Integrative Medicine, Jammu, India. The present studies focused on the phytochemistry of Dysoxylum binectariferum; dysoline, camptothecin and 9-methoxycamptothecin and medcinal chemistry and preclinical evaluation of IIIM-290 a semi-sythestic derivative of rohitukine.

Poor quality of water is one of the important factors causes gradual accumulation of salts on the ground or in the root zone of crops leads to saline and alkaline condition. This results in loss of permeability, toxicity and anaerobic conditions. In salt affected soils plants face constraint to derive their nutrients at the required rate due to high soil moisture tension, which affects adversely the crop growth and productivity.

10.1 Causes of Salt Problems

The following are the reasons for causing the soils become saline or alkaline.
1. Rise in water table: A rise in water table and high evaporation and transpiration rates with poor drainage lead to build up dissolved salts.
2. Quality of irrigation water: Irrigation water contains the dissolved salts exceeding certain limit cause accumulation of salts in soil profile.

12.1. Introduction

The protocols provides guidance on the structure and content of the International Plant Protection Convention (IPPC) diagnostic protocols for regulated pests. The protocols describe procedures and methods for the official diagnosis of regulated pests that are relevant for international trade. They provide at least the minimum requirements for reliable diagnosis of regulated pests.

This standard sets the framework for the content of diagnostic protocols, their purpose and use, their publication and their development. Diagnostic protocols for specific regulated pests are included as annexes to this standard. Information relevant for diagnosis is provided in the diagnostic protocol on the specified regulated pest, its taxonomic position, and the methods to detect and identify it. Diagnostic protocols contain the minimum requirements for reliable diagnosis of the specified regulated pests and provide flexibility to ensure that methods are appropriate for use in the full range of circumstances.

The methods included in diagnostic protocols are selected on the basis of their sensitivity, specificity and reproducibility, and information related to these factors is provided for each of these methods. Detailed information and guidance for the detection of pests is provided on, for example, signs and/or symptoms associated with the pest, illustrations (where appropriate), developmental stages of the pest, and methods for detecting the pest in a commodity, as well as methods for extracting, recovering and collecting the pests from plants.

Importance

It helps to cope with fever, cough and cold, stress, high blood pressure, excessive fats in body. It lowers the cholesterol level, cleanse the body by eliminating toxic substances, improve the digestive system, improve blood circulation, cleanses other organs of our body including kidney, pancreas, liver, bladder etc.and aid to deal with menstrual problems. The oil is distilled from leaves and flowering tops of Lemon grass. The oil has strong lemon-like odour, due to high percentage (over 75%) of citral in the oil. The characteristic smell of oil makes its use in scenting of soaps, detergents, insect repellent preparations. However, the major use of oil is as a source of citral, which goes in perfumery, cosmetics, beverages and is a starting material for manufacture of ion ones, which produces vitamin – A. The Citral rich oil has germicidal, medicinal and flavouring properties. An allied species called West Indian lemon grass (C.citratus) has low citral content in the oil and has meager trade in the country.

The global economy has adversely been affected to a considerable extent due to weather related disasters like droughts, floods, heat waves and cold waves which are not uncommon in the recent past due to global warming and climate change. For the first time, the year 1998 was declared as the weather related disastrous year globally. The year 2015 was the warmest year globally, again. Such weather phenomenon of warming more and more year after year was noticed in the current decade as well when we look at 2023 and 2024. The year 2010 was the warmest year ever recorded, followed by 2009 in India. The occurrence of floods and droughts, cold and heat waves adversely affected the Indian foodgrains production to a large extent across the Country in one or other region as seen in 2014, 2012, 2009, 2004, 2002 and 1987. The cold wave in winter 2003- 04 across the Northeast region of the Country and the heat wave in summer 2004 across the same region are still in memory to understand the ill effects in agriculture/horticulture/poultry/inland fisheries sectors. Model simulations
indicate that a marked increase in rainfall and temperature over India would be seen during the current century. The maximum expected increase in rainfall is likely to be 10–30% over the Central India. Temperatures are likely to increase between 2 and 30Cby the end of 2100 A.D. However, steps taken towards the green energy across the world may yield results not to allow atmospheric global temperature beyond 1.5 to 20C. Greater number of high surges and increased occurrence of cyclones in post monsoon period along with increased maximum wind speed are also expected along the East Coast of the Country. The summer droughts and heavy rains leading to waterlogging, floods and landslides during the monsoon period is a threat to plantation crops which are predominantly grown in the Humid Tropics like Kerala (8°15` N and 12°50`N latitudes and between 74°50`E and 77°30`E longitudes).

The potential value of any feed for supplying a particular nutrient can be determined by chemical analysis but the actual value of the feed to the animal can arrived at only after making allowances for inevitable losses that occur during digestion, absorption and metabolism. The first tax imposed on feed is that represented by the part of it which is not absorbed and excreted in the feces.

This may be defined as the factor present in feeds/ fodders or trees leaves and impair some aspects of animal metabolism, produce adverse biological effect in animal production. These are referred mostly as toxic factors because of the deleterious effects they produce when taken by the animals. The term ‘toxic factor’ however, is misleading because there is an implication that the substances are lethal beyond certain level of intake.

I. IMMUNOLOGY OF MALE REPRODUCTIVE SYSTEM

There are two basic functions of the immune surveillance of man and animals with respect to sperm immunity during spermatogenesis and fertilization process: support of the elimination of the vast majority of 'waste "spermatozoa that fail to fertilize and preservation of the tiny minority of surviving spermatozoa that are able to fertilize the oocytes. The primordial forms of spermatozoa arising in the seminiferous tubules of testes are preserved by the blood-testes barrier which is formed by sertoli cells ringing the semineferous tubules (Hunter, 1989). During maturation in the epidydimis, the spermatozoa acquire their antigenic properties. Simultaneously, the spermatozoa are coated with some proteins of seminal plasma having the immunosuppressive activity toward lymphocytes and macrophages involved in the immune reactions. The seminal plasma proteins accompany the migration of spermatozoa to the female genital tract (Matousek, 1985).

    1.    As per FSSAI, 2011 regulations, what is the fat and SNF content in buffalo milk
        a)    5 & 9    b)    6 & 8
        c)    6 & 9    d)    None
    2.    As per FSSAI, 2011 regulations, what is the fat and SNF content in  cow milk
        a)    4 & 9    b)    4 & 8.5
        c)    6 & 9    d)    None
    3.    As per FSSAI, 2011 regulations, what is the fat and SNF content in  standardized milk
        a)    4 & 9    b)    4.5 & 8.5
        c)    6 & 9    d)    None

Abstract

We studied the mathematic model of “predator-prey” and„ pest-parasite”. Under the circumstances when these two specious coexist in particular conditions. In that case the components of the system continuously change. We describe the system by two differential equations which conclude into numeral equation by integrators and variables. According these equations we calculate some numeral formulations essential to these changeable populations.
  
We produce the prevalence of predator-prey systems by using analytic and quantitatic methods for massive rage of data. This process as usual is described by the differential equation consisting assembling point. The constructed phase portrait which we need to solve the problem is found by Runge-Kuta method. So the article speaks about the conformity with a law which we receive by permanent change of parameters’ menaings and temporal changes of biological systems’ conditions.

When there is no positive steady state both prey and predator can become extinct. In one particular case both species become extinct regardless on the initial data. In the second, both species will die out only if initial prey/predator ratio is too low. Otherwise, only the predator goes extinct, while prey tends to carrying capacity.

Introduction

Baking is a traditional activity which has a long history, and according to some recent discovery, it is indicated to be dated back to as early as around 21,000 B.C. It is termed as a complex operation of prolonged heat transfer through radiation, convection, and conduction. Baking is considered to be one of the oldest methods of cooking, most commonly the bread baking but it is not only limited to the bread instead it provides a wide range of products such as cookies, pastry, pizzas, breakfast cereals, etc. Baking includes lots of raw materials such as flour, water, yeast, milk and milk derivatives, shortening, sugars, eggs and some minor ingredients such as malt products, cocoa products, dough improvers, inhibitors of microbiological spoilers, spices, etc. Food items which are rich in sugar and carbohydrates such as chocolates, candies are known as confections, and the art of making these products is termed as confectionary. Confectionery can be broadly classified into two major categories, namely sweet confectionary and baked confectionery. Conventionally cake was defined as the flour based sweet food, whereas bread was defined as the flou-based non-sweet food.

Baked confectionery, also known as flour confectionery includes products such as cakes, pastries, and similar baked goods, especially which are served in dessert. These delicacies hold history not much later to the bread baking. Earlier the baked confectionery especially cakes were only affordable by the rich and were prepared for special occasions such as festivity, celebrations, etc. but now a large number of flour confectioneries are being produced in the world which is delightfully acceptable and are much more in demand by a various group of people.

Carbohydrates are the macronutrients consisting of carbon (C), hydrogen (H), and oxygen (O) atoms. In simpler terms, carbohydrates can be defined as polyhydroxy aldehydes or ketones or substances, which yield them on hydrolysis. The general formula of carbohydrates is Cn (H2 O)n , where n is the number of carbon atoms. In nature, carbohydrates are the most abundant biological molecules. In the human diet, carbohydrates are one of the main nutrients that provide energy to the body. The green plants, through the process of photosynthesis, synthesize the carbohydrates.

Classification of carbohydrates

Carbohydrates are also known as saccharides, originating from the Greek word sakcharon, which means sugar. However, not all saccharides have a sweet taste. Carbohydrates can be classified into three distinct groups: monosaccharides, oligosaccharides, and polysaccharides. The number of sugar units determines this classification.

Monosaccharides

Monosaccharides, or simple sugars, are the most basic carbohydrates. They are the monomers that serve as the building blocks for forming polymers or complex carbohydrates. Monosaccharides are the most simple carbohydrates possessing carbonyl groups, i.e., aldehyde (CHO) and ketone (C=O) groups with two or more hydroxyl (OH) groups. Monosaccharide has the generic formula Cn (H2 O)n, where n is the number of carbon atoms. Monosaccharides are sugars that cannot be hydrolyzed further to form simple carbohydrates. These crystalline solids without color dissolve in water but remain insoluble in non-polar solvents.

Introduction Biochemical techniques are a set of methods/tools used to analyze and study biomolecules, cellular processes of body and disease diagnosis. The techniques form the cornerstone of modern research and are applied in various fields such as medical science, forensic science, drug discovery as well as environmental studies. Briefly, such techniques are used to isolate, analyze, and manipulate biomolecules such as proteins, nucleic acids, lipids, and carbohydrates. It is therefore imperative to understand the principle of biochemical techniques for pursuing a career in biological science such as Biochemistry, molecular biology or Biochemistry. Biochemical techniques offer several uses across multiple scientific areas such as: • Medical Research: In the identification of biomarkers, diagnosis of disease conditions such as diabetes and cancer and development of therapeutic interventions. • Pharmaceutical Industry: In this biochemical

INTRODUCTION

Custard apple (Annona squamosa L.) is considered as one of the delicious fruit meant for table purpose. In India, during the year 2014-15 the area under cultivation of custard apple was 29870 ha with production of 228370 tones and productivity of 7.65 tones ha-1 (Anon, 2015a). In Maharashtra, the area increased by 9 to 10 times recently i.e. since 1990 onward, after start of Economic Guarantee Scheme (EGS) of Government of Maharashtra. Hence, Maharashtra is the leading state in custard apple production, wherein during the year 2014-15, the area, production and productivity of custard apple was 8660 ha, 59330 tones and 6.85 tones, respectively. This accounts about 25.98 per cent share of the state in the national production. This is followed by Gujarat, Chattisgarh, Madhya Pradesh and Karnataka. (Anon, 2015a). The largest area of nearly one third of the Maharashtra State is in Purandar tahsil of Pune district. An average production of custard apple in Purandar area is about six tons ha–1. The farmers fetch high economic returns at very low input cost. Besides, export quality fruits get very high price ranging from Rs. 70 to 800 kg-1 (Anon, 1998) in foreign countries.

General Aspects About Insects

‘Insects’ are almost ubiquitous. They are found all over the world, in the icy regions of the poles, deserts, forests, mountain peaks, oceans and even in oil wells. They can adopt themselves to any climatic conditions and can live in any habitat where food is available. Insects are mostly small, air-breathing organisms of the animal world. Most of the insects live freely in nature. Many species are wild and harmless to human beings. Many are beneficial because of their role in the ecosystem. Some are a part of the food chains of wild creatures, while others through predation and parasitism hold in check many other species of insects. Insects also play a vital role in pollination. Relatively only a few species are harmful to man and are his chief competitor on earth.

Milk is the secretion of the mammary glands of animals. For human consumption, milk is that liquid obtained from the cow or the buffalo during the period following at least 72 hours after calving or until the milk is colostrums-free. It is a complex biological fluid consisting of seven main components: water, fat, protein, sugar (lactose), minerals, vitamins and enzymes. Milk is a white opaque fluid in which fat is present as an emulsion, protein and some mineral matters in colloidal suspension and lactose together with some minerals and soluble proteins in true solution. The opacity of milk is due to its content of suspended particles of fat, proteins and certain minerals. The colour varies from white to yellow depending on the carotene content of the fat. Skimmed milk is more transparent with a slightly bluish tinge. Milk of a good flavour has a pleasant, slightly sweet taste, and no odour. It is an excellent source of calcium, phosphorous and riboflavin. Vitamin A and D are currently added to whole milk, partly skimmed and skimmed milk.

Chapter Overview

This chapter delves into the concept and application of gender mainstreaming within the context of animal husbandry and the livestock sector. Gender mainstreaming is a comprehensive strategy aimed at promoting gender equality by integrating a gender perspective into all aspects of policies, programs, and projects. It recognizes and addresses the distinct roles, needs, and contributions of women and men involved in animal husbandry. This chapter provides insights into why gender mainstreaming is essential in this sector and outlines practical approaches for its implementation.

5.1 Participatory Approaches to Watershed Management

Community Engagement Strategies: Exploring methods for involving local communities in decision-making processes, project planning, and implementation, including participatory rural appraisal (PRA), stakeholder consultations, and community meetings. Exploring methods for involving local communities in decision-making processes, project planning, and implementation is essential for promoting ownership, sustainability, and effectiveness of development initiatives within watershed areas. Here are key community engagement strategies:

a. Participatory Rural Appraisal (PRA): PRA is a participatory approach that involves community members in assessing their own needs, priorities, resources, and capacities. PRA techniques, such as transect walks, social mapping, seasonal calendars, and wealth ranking, enable communities to identify and analyze local issues, strengths, and opportunities. PRA fosters dialogue, builds trust, and empowers communities to take ownership of development processes.

b. Stakeholder Consultations: Conduct stakeholder consultations to engage a diverse range of actors, including community members, local leaders, government officials, civil society organizations, and other stakeholders. Consultations provide opportunities for stakeholders to share their perspectives, knowledge, and concerns, and contribute to decision-making and project design. Stakeholder consultations ensure inclusivity, transparency, and legitimacy in development processes.

c. Community Meetings: Organize community meetings, village assemblies, or public forums to facilitate dialogue, consensus-building, and decision-making among community members. Community meetings provide platforms for discussing development priorities, reviewing project progress, and resolving conflicts collaboratively. They foster social cohesion, collective action, and community mobilization around shared goals and aspirations.

This chapter outlines fundamental molecular biology protocols essential for genomic analysis and molecular characterisation. Modern molecular biology relies heavily on precise analytical techniques for studying genetic material. Hence, this section presents a typical workflow encompassing nucleic acid extraction, amplification, and subsequent analysis. These methodologies are fundamental to various applications in molecular biology, including phylogenetic analysis, gene expression studies, and molecular diagnostics.
The chapter covers four core molecular biology and bioinformatics techniques, each building upon the previous:
1. Genomic DNA Extraction: Protocols for efficient isolation of DNA from biological samples. This encompasses cell lysis, protein denaturation, and nucleic acid purification through standard separation techniques. The quality and purity of extracted DNA significantly influences downstream applications.
2. Primer Design with Primer3: In silico design of oligonucleotide primers for PCR amplification. This section covers primer design parameters including optimal melting temperature (Tm), GC content, primer length, and considerations for avoiding secondary structures and primer-dimer formation.