eChapters

MENDEL’S RULES OF GENETICS DO NOT ALWAYS WORK

Mendel’s rules:

Segregation of alleles

Independent assortment of genes for different characters

Hold for characters:

Which have dominant & recessive traits

Determined by single genes

Genes are on separate chromosomes

Different types of chromosomes, or

Different homologues of same type of chromosome

These conditions are not always met, so there are many complications

3.1 Introduction

Different types of farm ponds with their characteristics features are discussed in this Chapter. It is observed that farm ponds can be constructed at different sites starting from the plain areas with relatively flat slopes to valley terrain having steep slopes. The choice of a particular type of pond largely depends on the kind of water supply available and on the existing topography of the site selected. In general for valleys with bottom width less than 100 m, barrage ponds may work well whereas valleys with bottom width more than 100 m, diversion ponds find suitable.

3.2 Collection of Basic Information

Basic information on different data relating to the soil, climate, plant, topography, groundwater table, catchment characteristics, and livestock populations are to be collected for the area where pond has to be constructed. Estimation of water supply from different sources and water demand form the pond for various purposes are to be done and a pond water balance study needs to be done before designing a pond in a particular place.

Three types of water movement within the soil are recognized. They are

1. Saturated flow
2. Unsaturated flow
3. Water vapour movement

Saturated flow

This occurs when the soil pores are completely filled with water. This water moves at water potentials larger than 33 kPa. Saturated flow is water flow caused by gravity’s pull. It begins with infiltration, which is water movement into soil when rain or irrigation water is on the soil surface. When the soil profile is wetted, the movement of more water flowing through the wetted soil is termed percolation.

Hydraulic conductivity can be expressed mathematically as

Abstract

Omics arise from ome which means complete or totality. It refers to the collection of technologies used to investigate the roles, relationships, and actions of the various types of molecules that make up an organism’s cells. Omics technologies provide the tools required to investigate differences in DNA, RNA, proteins, and other cellular molecules between species and individuals within a species. These technologies comprise, genomics, transcriptomics, proteomics, metabolomics, ionomics, glycomics, lipomics, cultureomics, pharmacogenomics, metagenomics, foodomics, eigenomics, phenomics, etc. The inclusion of all omics domain is known as multiomics. In this chapter we describe from basic, type structure of omics to all the recent cutting edge technologies in multiomics.

The polymerase chain reaction (PCR) stands out as a highly potent technology within the realm of molecular biology. With PCR, particular sequences present within a DNA or cDNA template can undergo replication, termed “amplification,” increasing their quantity manifold, ranging from thousands to millions. This process entails the utilization of sequence-specific oligonucleotides, a DNA polymerase capable of withstanding high temperatures, and thermal cycling.

Real Time PCR, a variant of the standard PCR technique, serves as a prevalent method for quantifying DNA or RNA within a sample. Through the use of sequence-specific primers, the quantity of copies of a specific DNA or RNA sequence can be assessed. This is achieved by monitoring the amount of amplified product at each stage throughout the PCR cycle. Earlier cycles exhibit amplification if a particular sequence (DNA or RNA) is abundant in the sample, while amplification in later cycles indicates scarcity of the sequence.

Abstract

Studies were carried out on 110 seedling trees of aonla growing at Tikker area of district Sirmaur, Ghanatti area of district Shimla and Kandaghat & Arki areas of district Solan to know the extent of divergence for various fruit characters by using non-hierarchical cluster analysis. Maximum co-efficient of variability was observed for fruit volume (38.63 %). First component gave maximum Eigen root value (7.286) and maximum percent variation (45.54 %). All 110 genotypes got clustered into eight clusters and the distribution of genotypes from different location was random. Maximum genotypes (30) entered in cluster 3 and minimum (5) in cluster 8. Intra-cluster distance varied between 1.877 (Cluster 5) to 3.137 (Cluster 8). The inter-cluster distance ranged between 3.006 (Cluster 3 & 4) to 11.677(Cluster 5 & 8). Wider genetic base is the prerequisite for breeding to get better recombinants and segregants. So, genotypes of Cluster 5 & 8 can be used for future aonla breeding programmes.

Arterial diseases are important because of their serious effect on vital organs like brain, heart and kidney. Thrombosis is the most common complication of arterial diseases but hemorrhage and aneurysm formation may occur.

Chromosomes are giant molecules of DNA

Found in nucleus: never leave except in cell division

Carry hereditary units, genes

Two homologous copies in diplod cells

Unwind when replicating or making RNA

Wind up (condense) in cell division

Attach to spindle and separate in meiosis & mitosis

Crossing over in prophase of 1st meiotic division

Chromosomes are equally distributed in mitosis, meiosis

Gametes have half the number of chromosomes as body cells

Sister chromatids are observed to split apart longitudinally in anaphase

ABSTRACT

Free radical scavenging property and antimicrobial activity of Triphala, the herbal product made of equal proportion of Terminalia chebula, Terminalia bellerica and Emblica officinalis were evaluated. Ethanol extracts of the formulation were tested for its invitro antioxidant activity by using improved ABTS radical cation decolorizing assay and invitro antimicrobial activity against Streptococcus mutans (predominantly involved in bio-film formation) by both well-diffusion and turbidity method to determine its minimal inhibitory concentration. An effort was also made to correlate its antiplaque activity using an invitro assay (where conditions kept similar to oral cavity) with Triphala and two commercial toothpastes (Colgate and Pepsodent). The ethanol extract showed significant antimicrobial and antioxidant activity. Triphala showed effective antiplaque activity when compared with the other tooth pastes. The herbal extract effectively inhibited the bio-film formation and more antioxidant activity exhibited by the extract might protect the gum cells effectively from free radicals than the commercial toothpastes, having less antioxidant activity.

17.1. What?

The soil is prepared to a fine tilth, which is referred to as land preparation or preparatory cultivation. This enables proper sowing of seeds, planting of seedlings and precise application of fertilizers to the crop. Various implements are used to prepare the land. The basic ones are ploughs, harrows, cultivators or tillers, and land levellers. These implements are manually operated or by animals or tractors. Various improvements have been made to increase the efficiency of each one of the implements by research workers. Many products are also available to suit the local regions and soil condition.

17.2. Plough

It has been the basic implement used for decades in agriculture to open up and invert the soil for initial cultivation. While inverting the soil, it also buries the weeds and the remains of the previous crops and allows them to decay. In the process of inverting, it also aerates the soil. Ploughs were initially used with animal power. Later on, many improved implements with heavy weights have been developed. These are drawn by tractors.

Objectives

1. To know the methods of essential oil extraction of spices and condiments.
2. To know the various chemicals present in the essential oil of different spices and condiments.

Essential oil: Essential oils are the liquids that are isolated from plants when introduced to solvents. They are liquefied versions of the plants.

Extraction methods of essential oil

1. Steam Distillation
2. Solvent Extraction
   1. CO₂ Extraction
   2. Maceration
   3. Enfleurage
3. Water Distillation
4. Water and Steam Distillation

Twitching is a condition occurring predominantly on the surface of the fruit which is exposed to direct sunlight; however, it is not well understood what the source of the disease is. Growth in immature fruits cannot be detected during later stages of fruit maturity. On cv. Golden Solo Party there are a few examples. This is termed the “frog skin” or “fox skin.” It shows on the fruit’s surface as the Dark Brown Flecks. 

Observation recording: The students are advised to visit the different forests areas and identify various insects and pests damaging trees in various ways like leaf feeding (defoliator), stem boring (stem borer) damaging seeds (seed borer) etc and various pathogens.

Agricultural innovations are crucial for addressing the pressing challenges of food security, environmental sustainability, and economic development. Around the world, innovative practices and technologies are transforming agriculture by increasing productivity, enhancing resilience to climate change, and improving livelihoods. This article provides an in-depth exploration of notable agricultural innovations, spanning traditional practices, cutting-edge technologies, and holistic approaches. By examining these innovations and their impacts, we can gain valuable insights into how agriculture is evolving to meet global needs.

Introduction

Agriculture is undergoing a profound transformation driven by technological advancements, sustainable practices, and innovative approaches. Innovations in farming have the potential to enhance productivity, resilience, and sustainability, thereby addressing global challenges such as population growth, climate change, and food insecurity. This article examines pioneering agricultural innovations from different continents, highlighting their applications, benefits, and implications for the future.

   Q.1.    How milk sample is collected for diagnosis of mastitis?
    Ans.    At first the end of teat is cleaned with a cotton swab dipped with 70% alcohol, then the dirt and wax are removed from the external sphincter by pressure. After this milk sample is collected in screw cap bottle.

    Q.2.    When milk sampling should be done in suspected cases of tubercular mastitis?
    Ans.    Last few streams of milking is crucial in cases of tubercular mastitis.

    Q.3.    What is the effect of freezing of milk sample on bacterial count?
    Ans.    On freezing of milk, Arcanobacter pyogenis and E. coli counts are decreased, coagulase negative bacteria counts increases whereas, Streptococcus and Staphylococcus spp. counts may be unaffected or increased.

    Q.4.    Which media is suitable for initial isolation of bacteria from milk samples?
    Ans.    Blood agar. However for isolation Streptococcus agalactiae selective media is required

UNTIL THE 1940S BIOLOGISTS ARGUED ABOUT WHETHER DNA OR PROTEIN WAS THE MOLECULE OF HEREDITY

Nuclei observed to divide when cells reproduced themselves

Large amounts of both DNA & protein in the nucleus

Many though protein was most likely molecule of heredity

Proteins made of 20 different amino acids- could have more variety than DNA (made of only 4 different bases)

Principle

The principle is to extract plasmid DNA from bacterial cell suspensions and is based on the alkaline lysis procedure developed by Birnboim and Doly (Nucleic Acids Research 7:1513, 1979). It is a known fact that plasmids are much smaller super coiled DNA molecules than the much larger and less super coiled bacterial chromosomal DNA. This difference in topology enables selective precipitation of the chromosomal DNA and cellular proteins and separates them from plasmids and RNA molecules. The cells are lysed under alkaline conditions, which denature both nucleic acids and proteins, and when the solution is neutralized by the addition of Potassium Acetate, chromosomal DNA and proteins precipitate because it is impossible for them to renature correctly (they are so large). Plasmids renature correctly and stay in solution, effectively separating them from chromosomal DNA and proteins.

Measurement of soil aeration

Oxygen Diffusion Rate (ODR) - the rate at which O₂ in soil air is replenished. ODR decreases with soil depth.

Measurement of ODR in soil

Lemon and Erickson (1952) devised a method for measuring oxygen diffusion in the soil system with the help of platinum micro electrode

Principle

When a certain potential is applied across the platinum electrode and a reference electrode inserted in the soil, oxygen is reduced at the platinum surface. The electric current flowing between two electrodes is proportional to the rate of oxygen reduction. The later, in turn, is related to the rate of oxygen diffusion to the electrode. The oxygen diffusion rate (ODR) is calculated from the measured electric current with the following equation.

Nematode associated with insects are referred as entomophilic, entomogenous and entomophagous nematodes. Generally they belong to the superfamilies Tylenchoidea, Rhadbitoidea, Oxyuroidea and Mermithoidea of the phylum Nematoda.

Nature of parasitism

Christie (1914) divided the nematodes associated with invertebrates into three groups.

• Those nematodes which live in the alimentary tract of he invertebrates.
• Those nematodes which are more or less closely related to free living species and often have a combination of saprophagous and parasitic habits.
• Those nematodes which parasitizes the body cavity or tissue of their host.

Entomophilic nematodes are group of parasites that cause debilitation, sterility or death of insects. Entomophilic nematodes vary greatly in size and shape having the insects as intermediate or as definite hosts. Many group of insects are parasitised by nematodes.

1. Introduction

Organisms are known to experience stress when environmental conditions, such as temperature, photoperiod, and humidity deviate from their optimal range. Organisms that respire aerobically constantly generate reactive oxygen species (ROS) through metabolism and employ antioxidants to reduce excess ROS formation and maintain a redox balance (Juan et al., 2021). ROS play crucial roles in cell signaling (Sies and Jones, 2020), homeostasis, cell death, immune defense against pathogens, and the induction of mitogenic responses (Dröge, 2002). Therefore, optimal environmental conditions maintain a balance between ROS formation and antioxidant processes in all living organisms.