eChapters

45.1. Aim

To conduct practical on preparation of sauce from different fruits.

45.2. Theory

Sauces are of thinner consistency as compared to ketchups and contain TSS not less than 15oBrix. Pumpkin, tomato, apple, papaya and plum pulps etc are used successfully for preparation of sauces. Mixed vegetable sauce using pumpkin, tomato, chillies, carrots are sold as continental sauces.

45.3. Raw material, ingredients and utensils required

1. Fruits like Apple, tomato, papaya, pumpkin, plum etc are used for making sauce.

2. Stainless steel knives, de-corers, ladle and utensils for cooking and mixing, glass bottles, sterilization tank, Bhatti/LPG stove etc.

Tomato, apple, plum, mushroom, etc., are used successfully for the preparation of sauces. Sauces are of two kinds 1) thin sauces consisting of vinegar extracts of flavoring material like herbs or spices and 2) thick sauces which are highly viscous.
Sauces are prepared by maceration of spices, herbs, fruits, and vegetables in cold vinegar or by boiling. Thickening agents like pectin, carboxymethylcellulose,n etc., are also added to sauces to prevent the sedimentation of solid particles. The sauce is generally cooked to such a consistency that it can be freely poured without fruit tissues.
Procedure
Tomato fruits are washed, cut into pieces, and heated/boiled for about 10 minutes in their own juice in SS pan to soften the tissue. The heated fruit mass is passed through a pulper to separate skin and seeds. Half of the sugar and spices are placed in a muslin cloth bag which is then immersed in the pulp during cooking. Cooking is continued till the pulp is reduced to half of the original volume.

Principle

Equal volumes of all acids / bases containing their gram equivalent weights per litre of the solution react completely with each other.

More often, if the reagent is not a primary standard, a secondary standard solution must be prepared. The chemical is dissolved and made up or diluted to the desired concentration and then it is standardized against a primary standard or against another reliably known secondary standard solution. Such solutions are called as secondary standard solutions.

Aim

To know the methods of preparation of side bars for large animal Thomas splint fabrication

Procedure

Two strong dry matured bamboo splints are of more than actual length of Thomas splint are prepared, (figure 1)

Three nicks at a distance of 2 inch are grooved on the side of the side of the splint at one end of the splints for keeping the tying end of the ropes in position (figure -2)

The bamboo splints at the lateral ends are thinned to some extent. For knob kniiting of splint to the metal ring by making an arc (figures).

All the steps are depicted in figure 1 -4.

Before fixing the metal ring with the bamboo extention splint the thinned ends are emersed inside water for easy arching of the bamboo splint.

Objectives

• Better understand the process and different stages of meiosis.
• Prepare your own specimens of onion root in which you can visualize all of the stages of meiosis.

Principle

Meiosis is a type of cell division in which the number of chromosomes is halved (from diploid to haploid) in the daughter cells, i.e., the gametes. It occurs in cell that produce gametes in sexually reproducing organisms. The division is completed in two phases, meiosis I and meiosis II. Meiosis I is a reductional division in which the chromosomes of homologous pairs separate from each other. Meiosis II maintain same number of chromosomes, hence, is called as equational division. Stages of meiosis can be observed in a cytological preparation of the pollen mother cells of the anthers of f lower buds.

Introduction
Ocular micrometer and stage micrometer are used to measure the size of microorganisms. The Ocular micrometer consists of a circular disk of glass which has graduations engraved on surface. A stage micrometer or objective micrometer has scribed lines on it that are exactly 0.01mm (10 micrometer) apart. Ocular micrometer is calibrated for the objective first. To calibrate the ocular micrometer for a given objective, the two scales are superimposed and determined how many of the ocular gradation coincide with one graduation on the stage micrometer scales. Thus, the distance between the graduations on the ocular micrometer can be determined and so the size of the organism can be determined.
Materials
1. Ocular micrometer
2. Stage micrometer
3. Microscope
4. Stained slides of yeast or bacteria

Object: Preparation of solutions. 

Material required: Volumetric flask, measuring cylinder, beakers, pipettes, balance, distilled water and chemicals. 

Theory: The use of solution is common in experimental procedures of plant physiology. It is very important for a student to be familiar with their preparation. The substance dissolved in a solution is called solute and the medium in which it is dissolved is known as solvent. Solutions of two liquids (e.g. alcohol in water) and solid in liquid (e.g. NaCl in water) are most common. Solutions are thus a homogenous mixture of a solute in a solvent. 

35.1. Aim

To prepare squash from fruits or fruit pulp.

35.2. Theory

Squash is a type of fruit beverage containing at least 25% fruit juice or pulp and 40% total soluble solids (TSS). Squash is diluted before serving (1:3). According to FSSAI specifications, class II preservative in fruit squash shall not exceed 350 ppm sulphur dioxide/700 ppm KMS or 600 ppm sodium benzoate. Artificial colour and flavours can also be added when declared on the label.

35.3. Raw material, ingredients, machinery and equipments required

1. Fruits like mango, orange, lemon, lime, litchi, pear, apricot and pineapple are used for making squash.

1. Preparation of Standard Sodium Hydroxide Solution

 Introduction

 When oxalic acid reacts sodium hydroxide, sodium oxalate and water are

form. Phenolphthalein indicator detects the end point by its characteristics

colour change.

 Apparatus

 Burettes, pipette, volumetric flask, beaker

 Reagents

 • Oxalic acid crystals

 • Sodium hydroxide – 0.1 N approximate

 • Phenolphthalein solution – 0.1 percent in ethyl alcohol

Procedure Preparation of 0.1 N oxalic acid solution 1. Weight accurately 1.575 g of oxalic acid into a 250 ml beaker using a weighing bottle. 2. Add some distilled water to the beaker and dissolve the oxalic acid with the help of glass rod. 3. Transfer the solution into a 250 ml volumetric flask through a funnel. 4. Wash the beaker 3- 4 times with small quantities of distilled water and transfer the washing into the flask. 5. Make up the volume up to the mark with distilled water and mix thoroughly. 6. Preparation of approximately 0.1 N sodium hydroxide solution 7. Weigh about 4.25 g of sodium hydroxide pellets and dissolve into 200 ml of distilled water.

Need of Analysis Technique

There are various methods to determine the concentration of a substance present in the matrix of an organic matter, these methods are very expensive, require expertise, need skilled man power, expensive chemicals and sophisticated equipments. But the volumetric analysis requires very less equipment and its level of accuracy is fairly high. It is very beneficial in the preparation of normal and molar solutions which is the back bone of all the research. Scientific venture is useless without the preparation of accurate standard solutions which is to be used in the estimation of various substances by sophisticated techniques like colorimetry, spectrophotometry, atomic absorption spectrophotometer (AAS), inductively coupled plasma emission spectrophotometer (ICP) etc. So the emphasis has been laid on the preparation of quick and accurate preparation of the standards. Volumetric analysis is the simplest technique by which, we can determine the concentration of a substance in solution form by titration of unknown solution against a known concentration solution. One solution is called the standard (known concentration) and other is called unknown solution. In this, reaction takes place either

Introduction

Subculture is key skill in bacteriological laboratory allowing us to colony propagate a pure culture in single colony. Pure cultures are essential for characterization such as identification using biochemical test or antimicrobial susceptibility testing.

Protocol

1. Label your fresh blood agar plate.

2. Select a single colony to subculture (colonies grows by clonal expansion; each colony should be a pure strain.)

3. Use the colony you have just picked to make the 1st streak on your fresh plate.

TV Talk
The talk delivered through TV i.e. through an electronic audio-visual medium which provides pictures with synchronized sound. The medium is cosmopolite in nature and can be used to develop instant mass awareness.

Important points while writing a programme for broadcasting
• Writing should be catchy, readable, conversational sound, understood and absorbed by the audience.
• Writing should be in simple, known language avoiding too much technicalities
• Interest of the listener should be sustained continuously
• Application of figures should be done judiciously
 

THEORETICAL ORIENTATION

One of the training methods used in management training and technical training is workshop method. The word workshop is borrowed from the area of industry. As the word says, in the workshop of industries, practical work is done by the workers. Similarly, through workshop method, some works are done by the participants who are actively involved in the same. One component which is common for all the methods is the person who conducts the proceedings of the method. We may call him a trainer or more appropriately for this method the director of the workshop. In using workshop method, the trainer needs a few experts who may be called as consultants or co-trainers. The trainer cannot handle the workshop situation single-handed.

A workshop schedule is very much important for planning an effective workshop method. It will help arrange all the necessary materials and the people well in advance to organise the workshop programme successfully and achieve the objectives of the workshop for which it is organised.

Materials Required

Tomato fruits, pulper, steel pans, weighing balance, refractometer, sugar, salt, spices, acetic acid, citric acid, muslin cloth etc.

Procedure
I. Puree

    1.    Extract fruit pulp by Hot process as told by the teacher.
    2.    Record the TSS of the pulp.
    3.    Concentrate the pulp by heating to 12º Brix (heavy puree)
    4.    Hot fill the boiling puree into pre-sterilized glass bottles (200 ml cap.)
    5.    Seal the bottles by crown corking
    6.    Heat process in boiling water in a big aluminium pan for 15-20 min. 
    7.    Cool the bottles to room temperature as quickly as possible.
    8.    Label and store at a dry place.

The Tris citric acid egg yolk glycerol extender is the most frequently used extender in the cryopreservation of cattle and buffalo semen. Preparation of the extender plays a very important role in determining the quality of the processed semen. Therefore, care must be taken to ensure that the extender is prepared aseptically and it contains all the ingredients in appropriate concentration. Extender should always be prepared under a laminar air flow. It is also important to check that the pH and osmolarity of the semen extender for a particular species corresponds to the prescribed limits for that particular species. The Tris citric acid egg yolk glycerol contains the following ingredients:

Objective: Preparation of Turmeric powder
Turmeric (Curcuma longa L), the ancient and sacred spice of India known as ‘Indian saffron’ is an important commercial spice crop grown in India. It is used in diversified forms as a condiment, flavouring and colouring agent and as a principal ingredient in Indian culinary as curry powder. It has anti-cancer and anti-viral activities and hence finds use in the drug industry and cosmetic industry. 'Kum-kum', popular with every house wife, is also a by-product of turmeric. It finds a place in offerings on religious and ceremonial occasions. A type of starch is also being extracted from a particular type of turmeric. The increasing demand for natural products as food additives makes turmeric as ideal produce as a food colourant. India is the largest producer, consumer and exporter of turmeric in the world.

A puppet is an object resembling a human, animal, or mythical figure, animated or manipulated by a puppeteer. Puppetry, an ancient form of theatre dating back to the 5th century BC in Ancient Greece, encompasses a wide array of puppet types, crafted from diverse materials to suit various forms and purposes.

Puppets vary greatly in complexity, from simple constructions to intricate designs. The puppeteer controls movements using hands, arms, or devices like rods or strings, manipulating the puppet’s body, head, limbs, and sometimes its mouth and eyes. Often, the puppeteer provides the character’s voice, synchronizing speech with puppet movements, which together convey narratives and stories.

Among the simpler puppet types are finger puppets, miniature figures fitting onto a single finger, and sock puppets, created and animated by inserting a hand into a sock, manipulating the hand to simulate the puppet’s mouth movements. Hand puppets, including live-hand puppets operated by one hand inside the puppet, and larger versions like live-hand puppets requiring two puppeteers, also feature prominently.

47.1. Aim

To conduct practical on preparation of pickle from different vegetables.

47.2. Theory

Pickles are good appetizers and add to the palatability of a meal. Pickles may be unfermented (mostly Indian pickles) and fermented (western pickles). Salt, vinegar, edible oil or acetic acid acts as preservative in pickle making. Mixed vegetable pickle from cauliflower, carrot and turnip is most common. Cucumber and olive pickles are examples of fermented pickles. The process of steeping vegetables/fruits in salt solution is also a common method used for preservation of mango and vegetable slices for longer duration storage for its later use in pickling.