eChapters

Potato is a major food crop, grown in more than 100 countries in the world and it is one of the major commercial crops grown in India. India ranks third by contributing around 7.5% to the world’s production. During 2006-07, 27 million tonnes was produced in an area of about 1.5 million ha. However, potato consumption per capita in India (14.8 kg/head/year) is one of the lowest in the world and hardly one per cent of the potato is processed.

Pulses are important food crops due to their high protein and essential amino acid content. Pulses are used as food and animal feed. India is the world’s largest producer, importer and consumer of pulses. Many exporting countries are dependent on India for marketing their pulses. Even though India is the world’s largest producer and consumer of pulses still there is a gap of 2-3 million tonnes between the production and consumption of pulses in the country and the gap is met through imports.

The first use of rubber was by the Olmecs, who centuries later passed on the knowledge of natural latex from the Hevea tree in 1600 BC to the ancient Mayans. They boiled the harvested latex to make a ball for a Mesoamerican ballgame.

Prices of major spices such as chillies, jeera, turmeric and cardamom are being driven to the ground by higher production, poor export earnings and a lack of working capital with traders to hold the stocks. Last December, all these spices were at record highs. With the prices ruling high last year, the acreage of most of the spices has increased this year, indicating excess production. The chilli acreage has increased by 15% to 20% while the land under jeera crop is estimated to have gone up by over 26%. The turmeric production is heading for a historic high of 82 lakh bags (each bag of 70 kg) and cardamom is having a bumper harvest.

Sugar originated from the Arabic word “sharkara” and is derived from the sanskrit world “sharkara”. Sugar is an important part of the daily diet and  forming a class of edible substances which includes  sucrose, lactose, and fructose. It provides the human body with requisite carbohydrates and is basically extracted from sugar cane and sugar beet.  Found in fruits, honey, sorghum, sugar maple and in several other sources, it is the main ingredient of candy which is loved by children the world over. Yet, it has been  blamed for causing tooth decay and excess consumption of sugar has been associated with a host of ailments like diabetes, obesity, weight gain, depression, joint pain, fatigue and insulin resistance and even cancer.

Random workplace surveys in the UK have proven that the staff can get pretty steamed up over hot drinks. Nine years ago, London Underground staff, in fact, threatened to go on strike when kettles were introduced to replace tea urns. Their grouse: it will make them late for shifts. When the credit crunch dried up the free watering holes in many companies, a survey by uSwitchforbusiness.com in 2010 claimed that trouble was brewing. It warned: "Cutting out the free cuppas may add to the bottom line, but there may be a far greater price to be paid in staff morale." Tea or coffee - free or not - is the preferred poison at work.

Fats and oils are one of the five essential ingredients of human diet. The four others are protein, carbohydrates, minerals, and vitamins. In balanced diet, the requirement of oils and fats per person per day is around 35 gm. In India, the major source of oils and fats is of vegetable origin; butter from animal milk also serves as a fat, though. In the nine major oilseeds, which are groundnut, rapseed /mustered seed, soya been, seasam seeds, sun flower seed niger seed, castor seed and linseed, out of these   seven are essentially edible. Among these seven, by far the most important are groundnut,  rapeseed/mustard seed, and soya bean. These three edible oilseeds account for a little over 80% of-the-total production of nine oilseeds, which include even two non-edible oilseeds, namely castor seed and linseed. As a matter of fact, edible oilseeds as such contribute nearly 95% to the total production of all cultivated oilseeds together.

ABSTRACT

The chemical characteristics of the groundwater in and around the Valapadi taluk of Salem district have been studied to evaluate the suitability of water for domestic and irrigation uses. Twenty three groundwater samples representing the shallow and deep aquifer of the area were collected and analyzed for pH, TDS, Ca, Mg, Na, K, HCO3, CO3, SO4 and Cl. It is observed that the quality of the groundwater is suitable for domestic use with some exception. The observed high quantity of Cl, SO4 and Mg at some places require immediate attention and detailed study. The value of EC and SAR indicate good to medium q of water for irrigation.

Primary cells are often preferred to established cell lines as they better represent the cells in vivo in terms of activity and functions. On the other hand, there is significant difference between original culture and present culture and even between cultures of the same cells in different laboratories. The rate of growth increases and there is change in chromosome numbers of lines over the time. The differences in phenotypes can cause problems in comparison and interpretation of results. The advantage of cell lines are ease handling, grow continuously, large yield and readily available. Primary cell cultures compared to established cell line are more fastidious in their growth requirements because many cell lines are derived from tumour tissue and demonstrate reduced growth factor requirements.

Primary processing of fruits and vegetables refers to the first steps in transforming raw agricultural products into forms more suitable for consumption, storage or further processing. This step generally includes cleaning, sorting, grading, cutting, peeling and packaging of the products. The aim of primary processing is to preserve the nutritional quality, taste and appearance of the products by extending their shelf life and making them more suitable for consumers or ready for secondary processing. For example, fruits may be peeled, cut or peeled, and vegetables may be washed, chopped, or blanched. This step is essential in the agribusiness industry because it has a direct impact on the quality and safety of the final product, ensuring that it meets market requirements, food safety standards and consumer preferences. The efficiency and methods used in primary processing significantly affect overall profitability, product quality and market competitiveness.
Primary processing refers to the initial stages of preparing fruits and vegetables for consumption while preserving their nutritional value, freshness and flavour. This essential step involves various techniques for cleaning, sorting and packaging the produce, ensuring that it reaches consumers in the best possible condition.

OVERVIEW OF PRIMARY TILLAGE EQUIPMENT
Primary tillage is the first and one of the most important steps in soil preparation for agriculture. It involves the mechanical disturbance of the soil to break it up, loosen it, and turn it over, creating optimal conditions for crop growth. The aim of primary tillage is to ready the soil for sowing by forming a seedbed, enhancing soil structure, managing weeds, incorporating crop residues, and improving water absorption. Loosening the soil also promotes aeration, which is crucial for root growth and microbial activity. The most common tools that are used for primary tillage are the mouldboard plough and the disc plough, each suited for different soil types and field conditions. These tools are designed to break and loosen the soil, ensuring proper root development and preparation for subsequent stages of cultivation.
The mouldboard plough, one of the oldest and most widely used primary tillage tools, includes several components such as a frame, shares (blades), mouldboards, landsides, and handles. The shares cut into the soil, while the mouldboards flip the soil over, creating furrows and burying crop residues. It is best for deep tillage in lighter, well-drained soils and is generally used in areas with low weed pressure. The depth and width of the cut can be adjusted to suit the soil type and crop requirements.
In contrast, the disc plough uses rotating discs to cut into the soil. Unlike the mouldboard plough, which fully turns the soil, the disc plough slices and fragments it into smaller pieces. This makes it particularly effective in dense, compacted, or rocky soils. Disc ploughs are ideal for regions with heavy, sticky soils or where crop residues are plentiful. The discs cut the soil without fully turning it, which helps reduce soil erosion and preserve moisture levels. Disc ploughs come in single-way and double-way versions, with discs mounted at different angles to improve soil penetration. Both mouldboard and disc ploughs are crucial for primary tillage, but

Abstract

Real-time PCR, also known as qPCR, is a highly sensitive technique used to determine the real-time amplification of a target DNA sequence. However, the presence of secondary structures or spurious amplicons can distort the results. When using SYBR Green chemistry, the features of qPCR primers differ from those of standard endpoint PCR primers. As a result, designing primers for SYBR Green qPCR requires extra caution and attention. This chapter outlines the essential features and conditions for designing qPCR primers, including primer length, melting temperature, GC content, specificity, and avoiding primer-dimer formation.

Abstract

It is essential that care is taken in the design of primers for PCR. Several parameters including the length of the primer, percentage of GC content and the 3' sequence need to be optimized for successful  PCR. Some of these parameters can be easily manually optimized while others are best done with commercial computer programs. In any event, careful observance of the general rules of primer designing will help ensure successful experiments.

Keywords: Bioinformatic tools, primer designing

Abstract

PCR primer design is a crucial skill for molecular biologists, as it enables the study of gene expression, gene amplification, and molecular markers. Primer3 is an online tool widely used for designing optimal PCR primers, which are pairs of short oligonucleotides that initiate DNA synthesis in vitro using a thermocycler. This tool uses a range of parameters to generate high- quality primers, including target sequence length, melting temperature, GC content, specificity, and product size. Understanding and effectively utilizing these parameters is essential for achieving accurate and reliable PCR results. In this chapter, the practical aspect of primer designing using the online tool Primer3 has been discussed. The parameters and the purpose of their usage have been given in a tabulated manner.

Primrose family is represented by about 58 genera and 2,600 species which are cosmopolitan but chiefly distributed in northern hemisphere. Some of the
larger genera with their approximately reported species include Primula (500), Lysimachia (200), Dodecatheon (50), Dionysia (41), Anagallis (30), Cyclamen (15), Salomus and Soldanella (11).
Selected Indian Genera
Primula, Anagallis, Androsace, Cyclamen, Primula.
Economic Importance
The family is of no specific economic importance except that of some well known decorative plants it contains. Some species of Primula may cause contact dermatitis. Anagallis arvensis is used for curing snakebite, leprosy, gout and even hydrophobia. Cyclamen purpurascence contains glucoside cyclamen which is poisonous. Some of the commonly cultivated ornamental plants of Primulaceae are the species of Primula (primrose), Androsace (rock jasmine), Cyclamen (Fig. 46.1), Dodecatheon (shooting star), Douglasia, Lysimachia (Loosestrile) and Omphalogramma.

In Doppler imaging, the reflections are from circulatory vessels, while in 2D ultrasonic image the reflection is from a tissue(s) or organ(s) In 2D ultrasound strength of reflection in B-mode depends upon density of the organ.On other hand the reflection in Doppler imaging depends upon direction of the circulation. If circulaltion is coming towards transducer, the signal will be strong, while it looses its strength while flowing away from the transducer. As shown in the diagram, the position of transducer on curved surface decides the strength or amplitude of signal. If it is positioned on curve end from where circulation is coming, the signal will be of higher amplitude, but when position is changed to other end of curved surface, where circulation is going down the amplitude of signal will decrease accordingly and is shown in reverse direction.

Principle of Doppler

Movement of blood in vessels is detected, which is depicted by color in some ultrasound machines. The flow coming towards transducer is shown above baseline, while flow going down i.e. away from transducer is shown below baseline. If position of transducer is changed from where the arterial flow is going away from transducer then, the arterial flow will be shown below baseline and venous flow that is coming towards transducer will be shown above baseline. Some machine shows red and blue color of the flow. The red color shows flow coming towards and blue color shows flow going away from transcer. A beginner ultrasonographer may think that the red color is from artery and blue is from vein. This is not true, this shows direction of flow. Since ultrasonic image of flow in Doppler is formed from series of pulses returning from vessels, the magnitude of pulse changes with variation in flow. A stationary flow will not show any height of magnitude.

Overhead Projector
Principles of Over Head Projector (OHP)
A projector, which projects the image over the head of an instructor on the screen, is called OHP. It worked on the principle of indirect projection. With the use of lens of short focal length and two mirrors the picture is reflected on the screen. The drawing, diagram, letter (may be in colour or in black and white) etc. are made on transparent sheet and are put on the glass platform of the OHP, through which strong light is passed. The rays of light are converged by a lens and reflected by a mirror held at an angle of 45°.

So far we studied direct stress (Tensile or compressive) and tangential stress (shear stress) acting on plane individually. But in many engineering problems these both stresses are acts on a plane at a same time. In such cases resultant stress on that plane will be neither pure normal nor purely shear but there will be combined effect of normal and shear stress.

Introduction
Biotechnology is a rapidly evolving field that harnesses biological systems, organisms, and cellular components to develop products and processes beneficial to humanity. In fisheries and aquaculture, biotechnology has revolutionized species identification, genetic enhancement, disease management, and sustainable production practices. By integrating molecular biology, microbiology, and genetic engineering, this multidisciplinary science has enhanced aquaculture productivity and contributed to food security. The advent of recombinant DNA (rDNA) technology, along with genomics, transcriptomics, proteomics, and genome editing, has transformed fish biotechnology by enabling precise genetic modifications, improving growth rates, and enhancing disease resistance.
A significant milestone in aquaculture biotechnology was the FDA approval of AquaAdvantage salmon, a genetically modified salmon designed to grow faster than its conventional counterparts. This approval marked the beginning of transgenic animal food production, demonstrating the potential of genetic engineering to enhance food production efficiency. In addition, genome-edited fish, such as the CRISPR-Cas9 modified tiger puffer and red sea bream, have been approved in Japan, further showcasing the advancements in gene-editing technologies for improving aquaculture species. These developments highlight the increasing acceptance of biotechnological innovations in fisheries, paving the way for more sustainable and productive aquaculture practices.This chapter focuses on the fundamental principles and methodologies of rDNA technology, molecular techniques, and recent advancements in biotechnology, emphasizing their implications in fisheries and aquaculture.