eChapters

Introduction

Plant pathogens induce diverse reactions in the body of their hosts. This result in creation of abnormalities which become visible on the plants. A visible or otherwise detectable expression of abnormal physiology, development, or behaviour in a plant resulting from disease is called symptoms. Normally, a distinction is made between symptoms and signs A symptom of disease is expressed as a reaction of the host to a causal agent, whereas, a sign is evidence of disease other than that expressed by the host. Signs are usually the structures of the pathogen. A disease is first noticed by the presence of symptoms and /or signs, and recognition of specific type of symptoms or sign aid in the eventual diagnosis of the disease.

The pioneering German microbiologist, Robert Koch, identified a set of four conditions that need to be satisfied to establish that a particular organism is the causative agent of a particular disease. These conditions, known as Koch’s postulates, are:

1. The suspected pathogen must be found associated with the disease in all the diseased plants examined.

2. The organism must be isolated from the diseased tissue and grown in pure culture on nutrient media, with its characteristics described (non obligate parasites) or in a susceptible host plant (obligate parasites), and its appearance and effects recorded

3.1 Introduction

To remove the inequality in technology development and its use, the concept Gender and technology arises. Economic resources should be equally distributed among the men and women. Technology can also help challenge and break gender stereotypes and biases that often limit women's potential. First of all, much equipment tends to be gender-typed. There are machines and tools suitable" for men as saws, trucks, wrenches, guns and forklifts. Some other technologies are "suitable" for women as vacuum cleaners, typewriters and food processors. The main focus of year 2023 is on innovation and technology for gender equality. Innovation and technological change are deep shifts reshaping life in the twenty-first century. Technological progress allows for unprecedented advances with the potential to improve social, economic and political outcomes for all.

Organic products are grown under a system of agriculture without the use of chemical fertilizers and pesticides with an environmentally and socially responsible approach. This is a method of farming that works at grass root level preserving the reproductive and regenerative capacity of the soil, good plant nutrition, and sound soil management, produces nutritious food rich in vitality which has resistance to diseases. India is bestowed with lot of potential to produce all varieties of organic products due to its various agro climatic conditions. In several parts of the country, the inherited tradition of organic farming is an added advantage. This holds promise for the organic producers to tap the market which is growing steadily in the domestic and export sector.

As per the available statistics, India’s rank 8th in terms of World’s Organic Agricultural land and 1st in terms of total number of producers as per 2020 data (Source: FIBL & IFOAM Year Book, 2020). The APEDA, Ministry of Commerce & Industries, Government of India is implementing the National Programme for Organic Production (NPOP). The programme involves the accreditation of Certification Bodies, standards for organic production, promotion of organic farming and marketing etc. The NPOP standards for production and accreditation system have been recognized by European Commission and Switzerland for unprocessed plant products as equivalent to their country standards. Similarly, USDA has recognized NPOP conformity assessment procedures of accreditation as equivalent to that of NOP of US. With these recognitions, Indian organic products duly certified by the accredited certification bodies of India are accepted by the importing countries. APEDA is also in the process of bilateral equivalence with South Korea, Taiwan, Canada, Japan etc.

Precision irrigation and fertilizer management in floriculture are revolutionizing the way flowers are cultivated, bringing together the latest advancements in technology to optimize resource use and enhance crop quality. In an industry where the aesthetics and health of flowers are paramount, precision agriculture techniques offer a tailored approach that meets the specific needs of each plant. Utilizing state-of-the-art tools such as soil moisture sensors remote sensing technologies and GPS-guided systems, precision irrigation ensures that water is delivered efficiently, reducing wastage and ensuring plants receive the exact amount of moisture required for optimal growth. Similarly, precision fertilizer management employs techniques like variable rate application (VRA) and soil nutrient mapping to provide plants with the necessary nutrients in precise amounts and at optimal times, avoiding the over-application that can lead to nutrient runoff and environmental harm. This method not only enhances the health and vibrancy of the flowers but also significantly contributes to sustainability in horticulture. By closely monitoring and adjusting water and nutrient inputs, growers can reduce their environmental footprint, conserving water and reducing chemical use. This is particularly important in floriculture, where the visual quality of the product is directly influenced by the growing conditions. With precision techniques, growers can achieve more consistent and high-quality blooms, which are essential for maintaining competitive advantage in the market.

The Sustainable Development Goals (SDGs), also known as the Global Goals, were adopted by all United Nations Member States in 2015 as part of the 2030 Agenda for Sustainable Development. These 17 interconnected goals aim to address global challenges and ensure a sustainable future for people and the planet. Here’s an overview of the SDGs
List of the 17 Sustainable Development Goals (SDGs)
1. No Poverty
2. Zero Hunger
3. Good Health and Well-being
4. Quality Education
5. Gender Equality
6. Clean Water and Sanitation
7. Affordable and Clean Energy
8. Decent Work and Economic Growth
9. Industry, Innovation, and Infrastructure
10. Reduced Inequalities
11. Sustainable Cities and Communities
12. Responsible Consumption and Production
13. Climate Action
14. Life Below Water
15. Life on Land
16. Peace, Justice, and Strong Institutions
17. Partnerships for the Goals
Aligning agricultural extension practices with the Sustainable Development Goals (SDGs) is essential for promoting sustainable agricultural development, enhancing rural livelihoods, and addressing global challenges such as poverty,

Historically, Indian scientists have recognized the importance of conserving plant biodiversity with particular reference to indigenous     and naturalized crops and their wild relatives. Indian scientists have recognized the importance of conserving plant biodiversity as early as 1935 by the ‘Crops and Soil Wing’ of the then Board of Agriculture and Animal Husbandry. Late Dr. B. P. Pal, in his classic paper ‘Search for New genes’ published in 1937 emphasized the importance of germplasm augmentation and utilization in crop improvement programmes. The need was reiterated in a meeting of the Indian Society of Genetics and Plant Breeding in 1941, which inter alia discussed the subject of economic crops. Dr. B. P. Pal, working at the then Imperial (now Indian) Agricultural Research Institute approached the then Imperial (now Indian) Council of Agricultural Research (ICAR) to set-up a unit for assembly of global germplasm under phytosanitary conditions in India. The ICAR scheme for plant introduction started functioning in 1946 under the leadership of late Dr. Harbhajan Singh as the first ‘Operational Scientist’. The scheme was further expanded and strengthened as ‘Plant Introduction and Exploration Organization’ in the Botany Division in 1956 and later established as a separate Division of Plant Introduction in IARI in 1961 headed by Dr. Harbhajan Singh.

Abstract

Powdery mildew infected leaves of apple hyperparasitized by Ampelomyces quisqualis were collected from field and examined for the presence of the dark brown pycnidia of  hyperparasite. The hyperparasite was isolated on potato dextrose agar medium by standard isolation technique. The morphological characters of the hyperparasite such as size and shape of pycnidia and pycnidiospores were studied. Six systemic fungicides were evaluated for their compatibility with A. quisqualis under in vitro conditions. Dinocap was least inhibitory giving 65.56 per cent growth inhibition of A. quisqualis followed by mancozeb (66.67%). Effect of these fungicides was also seen on conidial germination and germ tube length of A. quisqualis. Maximum conidial germination and germ tube length was observed in mancozeb followed by benomyl and dinocap.

To meet the food needs of the burgeoning population, India will need to produce 300 million tonnes of food grains by 2020. At present more than 75% of the total food grains produced in the country are of rice and wheat. Use of nitrogenous fertilizers has contributed much to the remarkable increase in production of rice and wheat in India that has occurred during the past three decades. During the last half-decade or so while fertilizer N consumption is touching new heights, the production of both rice and wheat is showing a trend of plateauing. In fact, fertilizer N efficiency of food grain production expressed as partial factor productivity of N (PFPN) has been decreasing exponentially since 1965. The PFPN is an aggregate efficiency index that includes contributions to crop yield derived from uptake of indigenous soil N. fertilizer N uptake efficiency, and the efficiency with which N acquired by the plant is converted to grain yield. A decrease in PFPN occurs as farmers move yields higher along a fixed N response function, unless other factors shift the response function up. In other words, an initial decline in PFPN is an expected consequence of the adoption of N fertilizers by farmers and not necessarily bad within a system’s context

Total protein: (Biuret method)

There are several methods of estimating protein, in food, agricultural and clinical samples, each of them varying in sensitivity and applicability.

Principle
Biuret method is highly specific for protein and is based on the formation of the blue peptide-copper complex in alkaline solution. It is a method of choice in the clinical laboratory because of the simplicity accuracy and precision. The major limitation of the method is its poor sensitivity for many dilute solutions. The principle of this method is that the peptide bonds in protein react with biuret reagent to give a violet colored complex known as cupric potassium biuret. The colour intensity of which is proportional to protein concentration.

In India, for centuries women have been pushed aside from the race of development in the name of customs, traditions and religion. As a result they have been denied the opportunities for their social, economical and political development leading to the lower status in society. The present situation demands active participation of women along with men in all walks of life. Involvement of women in all development activities. Based on the recommendations of National Policy of empowerment of women, Tenth Plan suggests a three fold strategies for empowering women through social empowerment, economic empowerment and gender justice.
 
Women play a significant and crucial role in agriculture and allied fields including crop production, dairy husbandry, horticulture, post harvest operations etc. It has been estimated that about 86% of the total rural women are working for various agricultural operations. They play an important role not only in maintaining their home task but also managing their farms and animals, depending upon the situation, personal and socioeconomic characteristics of the family to which they belong.  Women are also partners in animal husbandry activities management as manager or skilled workers.

The term empowerment has different meaning in different socio-cultural and political context and does not translate easily into all languages. The term include self strength, control, self power, self reliance, own choice, life of dignity in accordance with one’s values, capable of fighting for one’s right, independence, own decision making, being free, awakening and capability of mention only a few.

1. Introduction

Purple colour passion fruit (Passifora edulis Sims) is a tropical fruit and is well known for its nutritional content and medicinal properties (Patel, 2009). Various type of vitamin E such as α-tocopherol (5,7,8 trimethyl), β-tocopherol (5,8 dimethyl),g-tocopherol (7, 8-dimethyl),d-tocopherol (8-methyl) and α-tocotrienol, b-tocotrienol,g–tocotrierol and d- tocotrienol were quantified in passion fruit as antioxidant molecules with various health benefits (Ramaiya et al., 2013).

Foam mat drying convert thin pulp into stable foam by whipping. Eventually, the whipping process increases surface area, and speed up the drying rates which yield dried foam mat powder of acceptable quality in reduced time span (Kudra and Ratti, 2006). Rheology plays a vital role in food manufacture and marketing nowadays viz., design of handling systems, quality control and evaluation of sensory stimuli of viscosity (Barnes, 2001). It also concerns with how all food materials respond to applied forces and deformations. Basic concepts of stress (force per area) and strain (deformation per length) are keys to all rheological evaluations. Stress(r) is always a measurement of force per unit of surface area and is expressed in units of Pascals (Pa). The direction of the force with respect to the impacted surface area determines the type of stress. Normal stress occurs when the force is directly perpendicular to a surface and can be achieved during tension or compression. Shear stress occurs when the forces act in parallel to a surface. Various food shows different rheological behavior and categorized into solid and liquids stages. It basically means that food varies their characteristic in viscous and elastic behaviors commonly known as viscoelasticity of food, caused by entanglement of long chain molecules with other molecules (Abang, 2010). The dough rheology of various flour has been investigated by various research. Although, there has not been any published literature about dough rheology with incorporated passion fruit powder. To the best of our knowledge, there are no published reports regarding the shear stress properties of passion fruit powder incorporated rice dough.

Viruses are smaller than bacteria and require living cell for their growth. They can be detected through the bacteria to which they parasitise. Viruses are associated with both eukaryotes as well as prokaryotes and contain either DNA or RNA; only few have both DNA and RNA.

Viruses that infect bacteria are called bacteriophage. They consist of head or capsid and a tail. The head may be round, oval, polyhedral and is made up of proteins while tail is hollow and provides an exit for nucleic acid to enter in bacterial cell. The tail also helps in attachment to the cell wall surface of bacteria. After injection of the nucleic acid into bacteria they multiply and form large number of bacteriophage particles which cause lysis of the bacterial cell wall releasing many bacteriophages. Phages that cause lysis are known as lytic phages and that do not cause lysis are called temperate phage. The most common lytic phage is E.coli – a coliphage.

6.1. INTRODUCTION

All ecologist and biology researchers have to find out: (i) how reliable are the observed results? and (ii) what is the probability of difference between observed and expected results? The question of reliability is solved by estimating the confidence interval and the second question is addressed by the application of statistical tests for significance. Both these methods help us to draw conclusions on our observations, hence, may be grouped together as ‘inferential statistics’.

Referring back to the chapter on sampling, we may recall that it is usually not possible to calculate the ‘population parameter’ directly. Instead, we have to rely on estimates. Two types of estimates can be used, a point estimate and an interval estimate. A point estimate is a single numerical value of a sample statistic that is used to estimate the corresponding population parameter. For example, the sample mean, x , is used as an estimate of the population mean, μ Point estimates are not used widely because the value of some statistic, say mean, varies from sample to sample and we can’ t conclude with any degree of certainty that a single point estimate equals the population parameter. Therefore, an interval estimate is typically favoured. An interval estimate is a range of values within which the population parameter is likely to lie.

Self incompatibility in plants is a mechanism preventing the seed set by self pollination, and is probably way in which out crossing is enforced. Other factors may cause selfed seed may not to set seed due to embryo abortion, but SI is prezygotic and prevents embryo formation. SI is, therefore prevention of fusion of fertile male and female gametes after fertilization. Koelreuter first time in 18^th century reported S.I in Verbascum phoeniceum plants. In self-incompatible pollen grains fail to germinate on the stigma of the flower that produced them. Self incompatibility has been reported in more than 300 plant species belonging to 70 family of angiosperm. It is important out breeding device (cross pollination) for normal fruit set. It is maintained high degree of heterozygosity and can take place at any stage between pollination and fertilization.

Principle

Cell viability refers to the quantity of healthy cells in a sample, while cell proliferation is a crucial indicator for comprehending the mechanisms and pathways involved in the survival of cells or death following exposure to toxic substances. The methods used to assess viability are typically the same as those used to detect cell proliferation. Cell cytotoxicity and proliferation assays are commonly employed in drug evaluations to determine if the test compounds impact cell proliferation or exhibit direct cytotoxic effects. Cell viability can be determined by calculating the ratio of total live cells to the total number of cells (live and dead). Staining also helps to observe the general cell structure. Cell proliferation assay by MTT. Metabolically active cells reduce the yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) through dehydrogenase enzymes to produce NADH and NADPH. The purple formazan produced inside the cell can be dissolved and measured using spectrophotometry. The MTT Cell Proliferation Assay quantifies cell proliferation rate and detects decreases in cell viability caused by metabolic events leading to apoptosis or necrosis. The MTT Reagent produces minimal absorbance values when no cells are present.

Abstract

An experiment was conducted in two replications with seventy germplasm lines during kharif 2012 at College of Agriculture, UAS, Raichur. The germplasm included 30 local varieties from different parts of Karnataka and 40 lines collected from AICRP, Jabalpur. Each entry was sown in three lines of 4 m length with 30 cm x10 cm spacing. Out of seventy lines sixty two entries were germinated. These lines were evaluated for seed yield and other ancillary characteristics viz., days to 50% flowering, days to maturity, plant height, number of primary branches, number of capitula, number of seeds per capitulum and seed yield. Among them, six entries performed better than check variety No. 71 and 12 entries performed better than another check variety RCR 18. Among all the entries N-84 (238.89 kg/ha) and SD-33 (215.28 kg/ha) produced high yield. Hadagali local and No. 71 (51.5 days) were found earliest in flowering and maturing varieties and SP 141 was late in flowering (71 days) and late in maturing. Tavaregere local (66.5 cm) and SP134 had less plant height (67.5 cm) and BMD 127 had highest (113.5 cm) plant height. The number of primary branches was more in the genotype Humnabad local (12.5). Number of capsules per plant was more in Humnabad local and ICP 76 (53.0) and number of seeds per capsule were more in BMD 115 (77.0).

Abstract

Mango pulp is high in prebiotic dietary fibers vitamin c, polyphenols, carotenoids, vitamins and dietary minerals. Mint leaves are known for its medicinal aromatic and flavorsome qualities. Mint act as powerful antioxidant, protecting the body against the formation of cancerous cells and is rich source of polyphenolic compounds. Tulsi also have antioxidant, adaptogenic, anti-inflammatory, antibacterial and immune-enhancing properties. Honey is natural sweetener and has antiseptic and antibacterial properties. Medicinally, honey is non irritant promote rapid growth of healthy tissue and is useful in purities value, bed spores, skin and intestinal disorder, etc. Keeping in mind the beneficial effects of these raw materials, attempt was made to prepare value added drink from it, which may have medicinal value and good sensory acceptability with objective to prepare and evaluate the recipe for value added green mango-mint-tulsi beverage and to study the shelf life of the developed value added beverage. The formulation prepared with partial substitution of honey was more acceptable. Throughout the storage period, the squash prepared with honey scored more in flavor, taste, mouth-feel and overall acceptability than without honey. The cost of production without including labour, bottling and other overhead charges came out to be Rs. 34.5/L for squash without honey and Rs. 59.0/L for squash without honey.

Introduction

Functional food or medicinal food is any healthy food claimed to have a health-promoting or disease-preventing property beyond the basic function of supplying nutrients. The general category of functional foods includes processed food or foods fortified with health-promoting additives, like “vitamin-enriched” products. Fermented foods with live cultures are considered as functional foods with probiotic benefits. Functional foods are an emerging field in food science due to their increasing popularity with health-conscious consumers. The term was first used in Japan in the 1980s where there is a government approval process for functional foods called Foods for Specified Health Use (FOSHU) (Benkouider, 2005).

Distinguishing functional foods from dietary supplements and natural or herbal medicine is especially difficult, and cultural perceptions on this vary. Further complications are the large number of terms often used interchangeably and in a confusing manner such as health foods, nutraceuticals, designer foods, pharmafoods, and so forth. Much of the basic fortification (such as iodization of table salt) is no longer considered “Functional food” but new specialized extracts and ingredients for functional food fortification have emerged, including dietary fibres, plant sterols and probiotics. There is growing research on the potential of biotechnology to develop functional foods for improved health effects of the staple foods in developing countries—for example, high-iron rice, high-vitamin A rice, improved oil content in oil crops, and improved protein.

27.1 INTRODUCTION

In India, the horticulture sector contributes around 29 per cent of the GDP and 37 per cent of the total exports of agricultural commodities from about 13.08% of area. India has been placed as the second largest producer of fruits and vegetables; largest producer and consumer of cashew nut, tea, spices; third largest producer of coconut; fourth largest producer and consumer of rubber and sixth largest producer of coffee in the world. Several new programmes area in operation and significant developments in area expansion, production, value-addition and exports have taken place. Today, as a result of better synergy between research agencies, technological and policy initiatives by the governmental agencies, proactive participation of private players and higher degree of diversification to horticulture, this sector has emerged as a sustainable and viable proposition for even the small and marginal farmers including different stakeholders in the commodity chain. In spite of the remarkable progress made in the horticulture Sector in India, there is lot of scope in improvement in safety and quality aspects of horticultural produce.

The concept of biosafety encompasses a range of measures, policies and procedures for minimizing potential risks likely to appear to the environment and human health. Such safeguards must be put in place now. Biosafety is currently being promoted in a variety of ways by industry, governments and civil society. Quality and safety become prime concern because most of the horticultural produce are consumed daily in fresh, cooked and processed form and contribute major share in our daily diet. To ensure biosafety and quality of horticultural produce we need to ensure: a)Supply of quality inputs, b)Adoption of good cultural practices, c) Handling and processing of horticultural produce in scientific /hygienic manner, d)Packing, storage, transportation and delivery etc. in such a way that it reaches at consumers’ table without deteriorating the quality.

To ensure such aspects various preventive and certification measures have been initiated by government and certification agencies all over the globe including India , such as prevention of Food Adulteration Act 1954, Agricultural Produce Marketing Act, 1937, Food Safety Standards Association of India (FSSAI), Food Safety System Certification (FSSC:2200), International Food Certification (IFC), Food Chain Traceability, Hazard Analysis and Critical Control Point (HACCP), Food Product Orders (FPO), etc. The major emphasis under these certifications/regulations is to ensure quality and safety of the produce and minimize the risks by reducing the biological, chemical, physical hazards like microorganism contamination, level of heavy metal/chemicals/ drugs beyond the critical limits, residual effects of toxic substances etc. Brief of a few certification/regulations are listed below.