eChapters

Anaesthesia is an important criterion for doing laparoscopic procedure, which is being achieved by induction of general anaesthesia. However, in certain species, e.g. bovine, caprine etc., it can be done by preanaesthetic agent and infiltration of the local anesthetic agent at the site of the trocar placement. Selection of an anesthetic agent depends on the patient conditions, nature of procedure; time to be taken for the laparoscopic procedure and available facility. For the induction of the general anaesthesia follow the same procedure as in the conventional surgery viz. fasting, water withheld, preanaesthetic, fluid administration etc. Further, due to difference in the physiology of cardiopulmonary system of different animals, all these considerations varied from species to species and breed-to-breed. For instance cattle and buffalo are more prone to regurgitation and subsequent aspiratory pneumonia in general anaesthesia than those of dog and cat. While going for induction of general anaesthesia, an anesthetist should know about the different drug being used, their mechanism of action, effect on the different body system, their adverse effect and pure antagonist, if possible. Besides this an anesthetist should also know about the patient physiology and equipment for the administration of the anesthetic agents, because veterinary anesthetist is faced with numerous disturbances of cardiopulmonary and skeletal muscle function associated with general anaesthesia. For anaesthesia no anaesthetic agent is safe until and unless it is used by an experienced anesthetist.

Positioning of the patient/anesthesia

The patient is transported to the operating room and after general anesthesia the patient is placed in a desirable position. Some operating tables can be repositioned intraoperatively, allowing the surgeon to change the angle of the patient during the procedure. Special care should be taken to avoid nerve injury by malpositioning the patient, particularly along the popliteal fossa and brachial plexus. If any part of the patient is in contact with a metal object, patient may be at risk of electro surgical burns. General anesthesia is preferred for laparoscopy as it provides adequate muscle relaxation and assisted respiration particularly as the patient is in Trendelenburg position.

Laparoscopy is an operative procedure designed for the visual inspection of peritoneal cavity and biopsy of organs. Laparoscopy is a valuable diagnostic and therapeutic tool in clinical medicine. In the field of cholecystectomy, appendicectomy, vagotomy, hernia repair and adhesion release laparoscopy surgery offers significant advantages over open surgery in humans. For gynecological problems like ovarian cyst or in the case of oophorectomies and hysterectomies, laparoscopic surgery (‘scarless surgery’) is now considered a better alternative in addition to laparoscopic sterilization. Only in the last 15 years laparoscopy is widely used in different animal species for research and clinical diagnostic and therapeutic purposes. The first canine laparoscopy procedures were performed in the early 1900’s (Kelling, 1902). Anderson (1937) used dogs to perfect his skills prior to human clinical trials. The first clinical application of laparoscopy in dog’s involved ovarian function studies in the early 1960’s and Lettow reported the first laparoscopic liver biopsy procedure in a dog in 1972.

Laparoscopy is not only used in the examination of the abdominal cavity organs, surgical treatment but also in the diagnosis of the different disease conditioned by biopsy examination (removal of a sample of tissue from the living body for histopathological examination). Biopsy is indicated to ascertain a correct diagnosis, specific therapy and accurate prognosis. Laparoscopy is becoming an alternative method of monitoring biopsies of internal organs. It permits precise localization and accurate site on the various abdominal organs surface such as kidney, liver, spleen etc. Since, it is relatively noninvasive so it ranks highest amongst biopsy techniques. The technique allows visual control over the biopsy procedure, which considerably limits post biopsy complications and increases the likelihood of obtaining diagnostically useful biopsy specimens.

The most common indication for laparoscopy is to examine and biopsy the abdominal organs or masses. Additional diagnostic procedures that can be performed using laparoscopic guidance include gall bladder puncture to collect bile, contrast radiographic techniques for the diagnosis of bile duct obstructions, overdistension of gall bladder, portography and splenic pulp pressure measurement.

Among the various modern surgical methods, laparoscopy is now held as one of the most potent and promising aids for both diagnostic (laparoscopy) and therapeutic (laparoscopic surgery) use. Laparoscopy is an endoscopic procedure that bridges the gap between clinical evaluation and surgical exploration. The laparoscopic surgical techniques in both and human and veterinary medicine have grown tremendously. Laparoscopy requires a minor surgical intervention and it provides the only available practical means of making repeated direct observation of abdominal viscera. The advantage of surgical laparoscopy over the conventional open surgical laparotomy include faster patient recovery because of smaller surgical sites, decreased hospitalization, improved cosmetic, improved visualization of abdominal organs, lower postoperative morbidity with lower infection rates, and less postoperative pain and stress. Surgeons are commonly looking for more progressive and stressful surgical methods of sterilization in dogs, particularly in canine birth control programs in urban industrial areas. Demographically dense urban and industrial areas require effective animal birth control programs that can overcome the problems of hospitalization, postoperative complications and reduction in the overall cost of operation. In a large scale animal birth control program, the conventional methods of sterilization require a long period between capture of dogs and their release, due to the time taken for the surgical wounds to heal. In this aspect, keyhole surgery (laparoscopic surgery) can revolutionize the entire program, as it needs only a very small surgical wound, which usually needs no postoperative care or regular dressings.

Many surgeons have made attempts to develop minimally invasive surgical techniques with minimum blood loss and fewer traumas, to treat the disease process and minimize patient morbidity. Laparoscopic surgery may provide a wide field of its extensive application particularly in surgical sterilization of different animal species. Furthermore, high demographic urban and industrial area requires an effective animal birth control programme, which can overcome the problem of hospitalization, postoperative complications, and the overall cost reduction of operation. The sterilization of (both male and female) is effective measure in controlling the stray dog’s problem. Because the sterilization of either male or female alone is not effective and also sterilization of female consume more time when compared to male sterilization. To combat stray dogs in Animal Birth Control (ABC) programme, it is effective to sterilize the male also. Castration of male dogs by conventional open method has many disadvantages and postoperative complications such as hemorrhage, wound dehiscence, infections, maggot infestations and scrotal swellings etc. In a large scale Animal Birth Control programme, the conventional methods of sterilization requires a long period between capture of dogs and their release due to the time taken for the surgical wounds to heal. In this aspect, Keyhole surgery (Laparoscopic surgery) can revolutionize the entire programme, as it needs only very small surgical wound, which usually needs no postoperative care or regular dressings. It also avoids postoperative complications such as wound dehiscence, herniation, etc. and reduces the surgical stress to animal as well as recurring cost of each surgery.

Introduction

The most common indication for laparoscopy in zoo animal clinical medicine includes evaluation of liver disorders (e.g. cirrhosis, hepatitis, hepatomegaly), staging for cancer treatment (liver and peritoneal metastases), diagnosis of various peritoneal diseases (e.g. ascites), diagnosis of pelvic soft tissue diseases (e.g. ovarian problem, endometritis, pyometra,) and diseases of spleen and pancreas. The utility of laparoscopy in zoo animal practice is wide and it has been used in almost all the species with encouraging results. Laparoscopy is now most commonly used in zoo animal medicine to examine and biopsy of liver, gall bladder, kidney, spleen, pancreas, ovaries and prostrate. It provides an excellent means for visualizing the surfaces and colour. Even subtle color and texture changes can be readily detected. The liver is generally evaluated through a right lateral mid abdominal approach in canine and feline species of zoo animals. Abnormal gross liver changes that can be readily appreciated include hepatic lipidosis, glycogen leaden liver, metastasis or multifocal neoplasm, nodular hyperplasia, cirrhosis, cholangihepatitis, extra hepatic biliary tract obstruction etc. Using grasping forceps advanced through the second puncture cannula, the biopsy can be obtained quickly and safely in zoo animals. The pancreas can usually be visualized thorough a right abdominal approach and biopsy samples can be safely obtained.

Introduction

Laparoscopy is an operative procedure, performed through a keyhole opening with a rigid telescope that allows visual inspections and biopsy of the peritoneal cavity and its organs. Laparoscopy was first introduced in 1901 in human medicine. It has only been in the last 15 years that laparoscopy has been widely used in various animal species for research, clinical diagnosis and therapeutic purposes. Veterinary laparoscopy was widely used initially in the reproductive physiology in equine, nonhuman primate and various zoo and exotic species. The first clinical application of laparoscopy in dog involved ovarian physiology in the early 1960s, and Lettow reported the first laparoscopic liver biopsy procedure in a dog in 1972. The utility of laparoscopy in animal physiology particularly in reproductive physiology is wide and it has been used in almost all the species with encouraging results.

The use of sampling in making decisions about an aggregate (or population) is possibly as old as civilization itself. Sampling is first broadly classified into two categories known as subjective and objective. Any type of sampling which depends upon the personal judgement or discretion of the sampler himself, is called subjective sampling. But the sampling method which is governed by a sampling rule or is independent of the sampler’s own judgement is known as objective sampling. The main difficulty with subjective sampling is that the sampler is ignorant of the degree of representativeness of his sample or the accuracy of the final estimates of the population values obtained.

Objective sampling is further subdivided as non-probabilistic, probabilistic and mixed. In non-probabilistic objective sampling, there is a fixed sampling rule but there is no selection probability attached to the sampling unit e.g. selection of every 10th individual from a list, starting with the first, or selecting every 10th line in a paddy field. If however, the selection of the first individual is made in such a manner that each of the first 10 gets an equal chance of being selected, it becomes a case of mixed sampling, partly probabilistic and partly non-probabilistic). On the other hand, if for each individual unit there is a definite pre-assigned probability of being selected, the sampling is said to be probabilistic sampling.

2.1 Simple Random Sampling
The simplest and common most method of sampling is simple random sampling. In this procedure, the sample is drawn unit by unit with equal probability of selection for each unit at each draw. It is sometimes referred to as unrestricted random sampling.

3.1 Introduction
Out of all methods of sampling, the most commonly used procedure in surveys is the stratified random sampling. When population units are of heterogeneous nature and if we select a sample using SRSWOR scheme then the sample selected may not be the representative sample and the estimate worked out from that sample will not be the reliable estimate of the population parameter. The estimate of sampling variance of the sample estimator obtained from that sampling scheme may happen to be very high leading to get a less precise estimate of the parameter. In such type of population, to overcome these draw backs, we use another sampling scheme known as stratified random sampling which is defined as follows.

4.1 Introduction
In many surveys, information on an auxiliary (supplementary, ancillary or apriori) variable x, which is highly (positively or negatively)correlated with the variable y under study, is readily available and can be used for improving the sampling design. Stratified sampling and probability proportional to size (p.p.s) schemes are two examples of improved sampling designs, in which the use of data on auxiliary variable has been made. However in both the schemes, it implies that the information on auxiliary variable on individual sampling units is available prior to presentation of sampling design. In case, data on auxiliary variable for individual sampling units are not available but only the aggregate value for all units of auxiliary variable is available, the two schemes can not be used. In such a situation, the aggregate data on auxiliary variable can still be used at the time of estimation of parameters under consideration, provided the data on auxiliary variable for the sampled units can be easily obtained at the time of recording the values of the study variable. Such types of estimation are based on ratio, product, difference and regression methods of estimation.

5.1 Introduction
In random sampling it is assumed in prior that the population is divisible into a finite number of distinct and identifiable units and these units are known as sampling units. The smallest unit into which the population can be divide, is called an elementary unit (element) of the population. A group of such units is known as cluster. When such cluster is treated as sampling unit, then the sampling procedure adopted is known as cluster sampling. If the entire area containing the population under study is divided into smaller area segments and each element in the population belongs to one and only one such area segment, the sampling is sometimes known as area sampling.
Generally, identification and location of an element requires considerable time. However, once an element is located, the time taken for surveying a few neighbouring elements is small. Thus, the main function in cluster sampling is to specify clusters or to divide the population into appropriate number of clusters. Clusters are generally made up of neighbouring elements and hence elements within a cluster happen to be of similar nature. As a matter of fact, the number of elements in a cluster should be small and the number of clusters should be large. The required number of clusters can be selected either by equal or unequal probabilities of selection. All the units in the selected clusters are enumerated completely i.e. complete enumeration is made in all the selected clusters.

6.1 Introduction
So far we have considered the methods of sampling in which the successive units (elements or clusters) were selected with the help of random numbers. Now, we shall consider a new method of sample selection in which only the first unit of the sample is selected with the help of random number, the rest being selected automatically according to a predetermined pattern. The method is known as systematic sampling. It is operationally more convenient than simple random sampling and which at the same time ensures for each unit, equal probability of inclusion in the sample. The systematic sampling can also be termed as systematic random sampling because of the first unit of the sample being chosen randomly. The pattern usually followed in selecting a systematic sample is a simple pattern involving regular spacing of units.
Thus, suppose a population consists of N units and is serially numbered from 1 to N. Suppose further that N is expressable as the product of two integers k and n so that N=nk. Draw a random number i such that 1 < i < k and select the unit with the corresponding serial number and every kth unit in the population there after i.e. the sample consists the units bearing the serial numbers as

7.1 Introduction
In chapter 5, the cluster sampling has been discussed in which clusters were considered as sampling units and all the elements in the selected clusters were enumerated completely. It has been stated there that the cluster sampling is economical under certain circumstance but the method restricts the spread of sample over the population which results generally in increased sampling variance of the estimator under consideration. It is, therefore, logical to expect that the efficiency of the estimator will be increased by distributing the elements over a large numbers of clusters. Hence instead of observing all the units in the selected clusters, one may like to observe only few elements of the selected clusters. This will involve the sampling work to be done also within the selected clusters. If we do so, the process of selection will involve sampling work to be done at two stage, one for selecting the clusters where sampling units are the clusters and the second for selecting the units (elements) within the selected clusters where sampling units are the elements.
The process of sampling can be done at more than two stages. In this case the first stage unit will be larger than the second stage units and the second stage units will be larger than the third stage units and so on. Due to process of sampling in stages, such sampling is called “Multistage sampling.”

8.1 Introduction
We have already discussed in details how different sampling schemes can be employed to draw the representative samples suitable to many kinds of population whose sampling units differ in nature. From practical view points, however, it is often more important to be able to make decision about certain unknown characteristics of the population on the basis of informations available in the representative samples which are drawn from the parent populations. Such decisions taken, are known as the “Statistical decisions”. For example, we may wish to decide on the basis of sample data whether (i) One educational system is better than the other or not, (ii) A given six faced die is unbiased (balanced) or not, (iii) A new insecticide is really effective to control the attack of insects or not? etc. Such problems are dealt with in the theory of statistical decisions which uses the principles of sampling theory.

9.1 Introduction
In the preceeding chapter, we have studied the estimation part of statistical decision (inference) infered about the parameters of the population on the basis of information available in the sample observations drawn from the parent population under study. In the present chapter we discuss the problems related with the tests of statistical hypothesis and its significance about the population parameters under study. For understanding of the subject matter considered in this chapter, very clearly and easily, one must be familier with the statistical terminologies used in the subject matter. Hence we discuss the various notable terminologies with some suitable examples in a simple way for better understanding.

10.1 Normal Distribution
The normal distribution is the most widely used distribution in statistics. Almost all biological data including the agricultural data eg. crop yield, plant height, seed weight, seed size, protein percent in pulse, oil content in an oil seed etc are assumed to be normally distributed. In practice their distribution is rarely verified. In distribution theory, for large values of n, the number of trial (or sample size), almost all the distributions e.g. Binomial, poission, Negative binomial, chi square, student’s t, and Snedenor’s F distributions etc. are very closely approximated by normal distribution. It is a continuous distribution with mean and variance as its two parameters.

11.1 Introduction
In the preceding chapter 10, we have discussed in detail, the test procedures for which it has been assumed that the sample size n is large, (i.e n >,30). Those tests are also known as approximate tests or z tests. But in case when sample size n is small i.e. n<30, the approximate test fails to deal with such problem and we apply the theory of exact sample, for small sample tests. The exact sample tests however can be applied to large samples also though the converse is not plausible. In all the exact sample tests, the basic assumption is that the parent population is normally distributed.
In this part we will discusse in details the exact sample tests (based on exact sampling distributions namely snedekor’s F, student’s t and chi square (χ2) distributions. These distributions will be simply introduced in short because main emphasis has to be given only on the test procedures based on them.

12.1 Chi-Square Variate
The square of a standard normal variate is known as a chi – square variate or chi square statistic with one degree of freedom.

13.1 Introduction
In the previous chapters, we have considered different tests of significance based on normal distribution, F distribution, student’s t distribution and Chi-square (χ2) distribution which are frequently applied by the users of different disciplines of applied sciences and social sciences. In this chapter some more tests of significance are considered which have not been covered in those chapters. These tests are also based on t, F, Fisher’s z transformation, χ2 and some other test statistics are also discussed one by one in the following sections.

Abstract
Horticulture comprising a variety of fruits, vegetables, ornamental and medicinal crops has emerged in the recent times as a key sector of the Indian economy contributing substantially towards enhanced income, employment and welfare of the rural poor. A visible shift is seen in crop diversification across regions in favour of horticultural crops, especially vegetables over traditional cropping pattern comprising of cereals and pulses, since early 90s. Definitely higher profitability has been identified in comparison to cereals, increased urbanisation, enhanced living standards, besides the growing awareness for balanced nutrition as the main reason for such a shift. Commensurate with this trend has been a visible shift in enhanced R&D investments into horticultural R&D both from the public and private sectors. There is a growing need for the public sector research organisations like ICAR to increase their visibility and promote public private partnerships through innovative methods of research and development at this juncture.

Abstract

In recent years the quest for improving the quality of life has ventured into many aspects of utilizing plants for the aesthetic sense. One such is an increased utilization of flowers /flowering plants for beautification of homes, offices, gardens etc. The increased interest in use of cut flowers has brought challenge of bringing in varieties of natural flowers from different sources. India’s rich biodiversity also contributes addition of lot of new species to the cut flower industry. Banana and Plantains have been revered only for their utility as dessert fruit or staple food. But aesthetic dimension has never been much explored. Musa comprises more than 50 species and sub species traversed across the tropical and sub-tropical regions of the world. Musa Ornata is one such plant, native to north eastern regions of India, belongs to the family Musaceae and section Rhodochlamys. It draws its attention by virtue of its bright inflorescence and its long lasting nature. It is a medium statured plant, hence suitable for gardens also. It is a fertile wild species, multiplies mainly through suckers and through seeds to some extent.

Abstract

An experiment was conducted in two replications with seventy germplasm lines during kharif 2012 at College of Agriculture, UAS, Raichur. The germplasm included 30 local varieties from different parts of Karnataka and 40 lines collected from AICRP, Jabalpur. Each entry was sown in three lines of 4 m length with 30 cm x10 cm spacing. Out of seventy lines sixty two entries were germinated. These lines were evaluated for seed yield and other ancillary characteristics viz., days to 50% flowering, days to maturity, plant height, number of primary branches, number of capitula, number of seeds per capitulum and seed yield. Among them, six entries performed better than check variety No. 71 and 12 entries performed better than another check variety RCR 18. Among all the entries N-84 (238.89 kg/ha) and SD-33 (215.28 kg/ha) produced high yield. Hadagali local and No. 71 (51.5 days) were found earliest in flowering and maturing varieties and SP 141 was late in flowering (71 days) and late in maturing. Tavaregere local (66.5 cm) and SP134 had less plant height (67.5 cm) and BMD 127 had highest (113.5 cm) plant height. The number of primary branches was more in the genotype Humnabad local (12.5). Number of capsules per plant was more in Humnabad local and ICP 76 (53.0) and number of seeds per capsule were more in BMD 115 (77.0).

Abstract

Agricultural practices prior to the eighteenth century were completely dependent on crop landraces and their mixtures. The knowledge of plant genetics and its application to crop improvement programmes led to increase the production potential of agriculture. Contrary to this, the wonders of crop improvement have resulted in the erosion of genetic diversity of many crops in farmers’ fields, including wheat, due to the replacement of landraces and farmers’ old cultivars with modern high-yielding cultivars.

Wheat is cultivated over an area of 216.6 million hectare globally and the production is 674.9 million tons. The seven largest producers of wheat are China, India, the Russian Federation, the USA, France, Turkey and Canada. During 2012-13, India produced 92.46 million tons of wheat from 29.65m ha area with productivity of 31.19q/ha (4th Advance Estimates, Directorate of Economics and Statistics, Government of India). Three wheat species namely, T. aestivum L., T. durumDesf. and T. dicoccumSchubl are grown in India as commercial crops covering 95, 4 and 1 percent area, respectively. The cultivation of T. dicoccumis confined largely to the regions of Karnataka and Maharashtra.

Abstract

Genetic diversity of thirty two rice genotypes from different states of India was evaluated through Mahalanobis’s D2statistic under integrated fertilizer management for fourteen yield and quality characters. The genotypes were grouped in to seven different clusters. Cluster I had nine genotypes. Three genotypes each were included in cluster II, cluster IV and cluster VII, whereas six and seven genotypes in cluster III and cluster V, respectively while the cluster VI had only one genotype. The results revealed that the geographic diversity might not always be related to genetic diversity. Kernel length after cooking, days to maturity and kernel breadth contributed maximum towards divergence indicating their importance in choice of parents for the hybridization programme.

Abstract

The Directorate of Sorghum Research has explored 9 states for sorghum germplasm during 2000 – 2011. The sorghum landrace collections are mostly named with the village name prefixed to the landrace (ex. tongraligaon maldandi, sultanpur maldandi etc), landraces are also named based on the grain colour (ex. tella jonna, bili jola etc.) and size (ex. moti rabi jowar), ear head compactness (ex. mudda jola), use of the stem, ear head or grain (ex. dagdi, gundu, allur, broom etc.) for varieties utilities. The landraces are being grown by farmers over the years for many reasons. The moli jowar grains are attractive and fetch higher price in the market compared to other released varieties in Madhya Pradesh. Some are used for specific food preparations. The irungu cholam flour is boiled with water to prepare food. The mathappu cholam is used to prepare a jelly like food known as khali in Tamil Nadu. The beed maldandi and bidri from Maharashtra are used to make quality jowar roti. Karnataka’s kodumurugu jola and allina jola are used to make laddu’s and pappads respectively. Broomcorn jowar from Uttarakhand are used for roti, cattle and poultry feed making. The allur jola from Karnataka is used to prepare allu dish during the nagapanchami (festival of snake god). The pachcha jonna from Andhra Pradesh, barmuda local, deshi chari and gudli local from Rajasthan and bendri dagdi and khondya from Maharashtra are very good fodder types. Valsangh maldandi local, Vadgaon dagdi maldandi, tongraligaon maldandi, tongraligaon dagdi, sultanpur local dagdi, sultanpur maldandi, harni jogdi (dagdi), harni jogdi, chungi maldandi, musti local (Maldandi), chungi kuch-kachi, baddi jowar, chakur maldandi from Maharashtra, and sai jonna from Andhra Pradesh are considered by the farmers as drought tolerant landraces. In Andhra Pradesh, Koya’s (tribe) grow konda jonna. Lambada’s used to grow the maghi sorghum landraces extensively in the state. The tribal community mainly uses the accessions as food and some are used for both food & fodder. The accessions collected from the higher elevation and remote forest area is source material for pest & disease resistance. The accession IC 347574 is used as the tribal food. The accession IC 347583 is a guinea race and IC 347585 is a durra-guinea race sorghum collected at an elevation of 500 feet from the tribal village. In Gujarat,Gomati (tribe) grow deshi jonna landraces. In Madhya Pradesh, Rewa district is the maximum deshi jowar cultivation region. This area is commonly called “Junariwala Shetra”. Mehran, Swarguor, and Kushwaha are the main tribes cultivating the jowar in these regions. In Maharashtra, Korukoo’s tribe grows somgi and deshi jonna landraces. They cultivate sorghum with lablab, chillies, cotton, redgram, and tomato at the height of 650 – 870 m from MSL. Maximum diversity of the sorghum still exists in the disturbed forests. Due to extend of its natural variability, India exists as the secondary origin of sorghum.

Abstract

While harnessing the benefits of genetically modified (GM) crops, in terms of increased yield, better nutritional quality and resistance to biotic and abiotic stresses, it is important to undertake biosafety and risk assessment studies in a systematic manner to allay the concerns of their impact on the environment and especially on the genetic diversity. Since Bt cotton is the only commercialized GM crop in India, this paper reports on the monitoring of unintentional introgression of transgene(s)/ regulatory elements of four commercialized Bt cotton events, covering 99.5% of the area under cultivation, in 180 ex situ cotton accessions conserved in the National Gene Bank at National Bureau of Plant Genetic Resources (NBPGR), New Delhi, using polymerase chain reaction (PCR)-based assays. Based on the real-time event-specific and conventional transgene-specific PCR assays, no amplification signals specific to two major Bt cotton events MON531, MON15985; transgenes/markers/ regulatory elements of 4 widely cultivated commercialized Bt cotton events, i.e., cry1Ac, cry2Ab, marker genes, i.e., nptII (neomycin phosphotransferase), aadA (aminoglycoside-3’-adenyltransferase) and uidA (?-D-glucuronidase) marker genes, CaMV35S, nos (nopaline synthase) promoters and nos terminator were detected in any of the cotton accessions. The study shows that the cotton accessions conserved in National Gene Bank are GM free and the study can be used as a model for monitoring the adventitious presence of unintended mixing of cotton seed during seed production or harvesting in Bt cotton growing states of India. On similar lines, adventitious presence of transgenes can be checked in ex situ collections of different crops conserved in the genebanks.

Abstract

A profile study of organic pigeonpea growers was conducted during 2007-08 in Gulbarga district of Karnataka state. Two taluks namely, Gulbarga and Jewargi were selected based on highest area under organic pigeonpea cultivation. One hundred twenty respondents were selected with simple random sampling procedure, to know (1) the practices followed by farmers in organic pigeonpea cultivation and (2) the forward linkage activities followed by organic pigeonpea growers. The study revealed that majority of respondents (63.33 %) had medium level knowledge about organic pigeonpea farming practices, with regard to individual organic farming practices, majority of respondents had knowledge about the practices like recommended seed rate (81.60%), recommended sowing time (98.33 %), application of FYM (100%), crop rotation (96.67%), pheromone traps (98.33%), NPV (100%) and NSKE (100%). With regard to post harvest operations, cent percent of respondents graded the produce manually and jute bags used to store the produce, whereas, 71.67% of the respondents sold later when price was suitable in regulated market (90.00%) through commission agents (77.50%) and got price information by personnel visit/phone call to regulated market (81.67%) and others who visited the market (68.33%). But no farmer certified the produce and sold at premium price. With regards to personal characteristics, majority of respondents (31.62 %) were illiterate, 59.17 per cent were had joint family, 61.17 per cent were having low farming experience, 60.83 per cent of respondents were big land holders and 69.17 per cent of respondents possessed medium level of scientific orientation.

Abstract

Many people’s food and livelihood security depend on the sustained management of various biological resources that are important for food and agriculture. Agro biodiversity is the result of the interaction between the environment, genetic resources and management systems and practices used by culturally diverse peoples. The management of agro biodiversity by the indigenous communities is for a variety of reasons. The land use types, land use stages, aspect, soil fertility, availability of water and quality seed of crops etc. are essential variables which an indigenous farmer considers while cultivating a diversity of traditional landraces in the farm for food security. India is the largest producer of many kinds of millets, which are often referred as coarse cereals. However, realizing the nutrient richness of these grains they are now considered as nutria-cereals. Small millets, as a group includes several grain crops namely finger millet (ragi), proso millet, barnyard millet, italian millet, kodo millet and little millet. In the four decades since 1961, the area under millets declined by nearly 50% from about 18 million hectares to about 9 million hectares. During this time, production of millets declined from about 8.8 million tons to about 7.2 million tons; a decline of 18%. Tremendous decrease in area and production is directly or indirectly resulted in the loss of agro biodiversity of these crops as these crops are grown on marginal or rainfed areas without any fertilizers. Therefore, more emphasis should be given for maintaining bio diversity of these highly rich crops by increasing area under these minor millets and developing proper agro techniques for increasing its productivity.

Abstract

Rice is the staple and principle food of about half the human race and is often the main source of calories. Navara is a unique rice cultivar indigenous to Kerala, gifted with extra short growth duration. It is mainly used for the preparation of Navara kizhi, which is an effective remedy for rheumatic complaints, neuro muscular disorders and body rejuvenation. It is traditionally given as a supplementary diet to the under-weight and also consumed as a replenishing drink called ‘Karkadaka kanji’ during the monsoon season along with certain other herbal medicines.Despite the Navara is known for its special medicinal attributes to the producers, consumers and other actors of the value chain, there is a declining trend of Navara rice cultivation noticed (2000 acres to less than 50 acres) in the state (The Hindu, November 28, 2010), which entails the loss of biological and cultural diversity associated with this unique rice.The study attempted to identify and analyze the actors involved in the value chain of Navaraon the conservation perspective. The primary data required to analyze actors in the value chain were collected from the farmers, input suppliers, processors, retailers and consumers of Palakkad, through key informant method, focused group discussion, and structured interview schedules and analysed through Value chain mapping tool and SWOT analysis. The secondary data regarding the rice and its properties were collected from websites and publications. The study revealed that the cultivation of Navarashould be purely organic to preserve its medicinal value. The cultural practices except ploughing, such as weeding, manuring, harvesting etc. are done manually. Application of chemical fertilizers will increase the vegetative growth of Navaraand subject to lodging of plants and at the same it also affects the quality of rice.

Abstract

The present investigation on “Agro bio-diversity and genetics of fibre characters, yield and its components in intra and inter specific hybrids of allo-tetraploid cottons (Gossypiumspp. Linn.)” was carried out at College Farm, College of Agriculture, Rajendranagar, Hyderabad, to study the combining ability and heterosis for quality and yield characters. Further, an attempt was made to trace out best parents and crosses for future breeding programmes and also to know the gene action controlling the traits through generation mean analysis for designing appropriate breeding strategy. In this direction, seven lines and four testers were crossed in L x T design to generate 28 hybrids and evaluated with check NHH 44 to know the combining ability of parents and crosses. Since L x T design does not provide comprehensive picture on gene action governing the traits, the generation mean analysis through joint scaling test was done in four crosses for eleven characters. High PCV and GCV were recorded for number of monopodia per plant and seed cotton yield per plant and moderate estimates were observed for number of bolls per plant, boll weight. High heritability estimates coupled with high genetic advance were observed for number of bolls per plant, boll weight and seed cotton yield per plant. Estimates of components of variance and their ratios (?2 gca/?2 sca) indicated the preponderance of non-additive gene action for days to 50 % flowering, number of monopodia per plant, number of sympodia per plant, number of bolls per plant, boll weight and seed cotton yield per plant, whereas additive gene action was observed for the fiber quality traits viz., 2.5 % span length, uniformity ratio, micronaire value and bundle strength.

Abstract

The genetic divergence among 249 germplasm lines of maize was assessed by D2statistics based on nine parameters at Agricultural Research Station, Karimnagar. The germplasm lines were grouped into eleven clusters. Clusters I and II were the largest clusters with 73 and 79 genotypes, respectively. The highest inter cluster distance was observed between cluster X and XI followed by cluster V and XI indicating more variability in the genetic makeup of inbreds included in these clusters. Cluster V showed highest mean values for grain yield/plant and ear length. Cluster XI had the lowest mean values for plant height and ear height and these two characters showed maximum contribution towards total divergence among different characters. Based on inter cluster distances, inter crossing among the genotypes belonging to cluster V, IX, X and XI has the chance of getting high yielding hybrids with higher heterosis.

Abstract

Crop diversity play important role of sustainable development. A study on crop diversity in Andhra Pradesh was conducted during 2010-2011. In Andhra Pradesh, cultivated crop plant species resulted in augmentation of 140 species belonging to 132 genera and 46 families. The maximum species strength was observed in the Rayalaseema region (127 species belonging to 120 genera and 43 families), followed by Telangana region (125 species belonging to 114 genera and 43 families) and parts of Coastal Andhra region (118 species belonging to 103 genera and 46 families). Species richness was predominant in cultivated crops like vegetable crops (31 species belonging 25 genera and 12 families), Fruit crops (25 species belonging 18 genera and 14 families), commercial crops (23 species belonging 23 genera and 15 families) and Leafy vegetables (12 species belonging 12 genera and 8 families). The maximum species richness in families was found in Fabaceae (16 genera and 19 species), followed by Poaceae (14 genera and 16 species), Cucurbitaceae (11 genera and 14 species), Asteraceae (7 genera and 7 species) and Apiaceae (5 genera and 5 species). The present and the past similar studies observed that many more native species remain in agriculture than previously thought, under the right conditions. There is a need to establish mechanisms of control on introduction of exotic species in natural habitats. We also need to strengthen the legislation on the use of biodiversity and to establish a research programme of the economic potential of local flora and fauna as an alternative to introduction of exotic species to safeguard our traditional crop diversity.

Abstract

India is a country of villages (640930 villages) and 68.84% population of India is living in villages as per the All India Census, 2011. Dairy farming has been complementing the Indian agriculture since ancient times and rearing of livestock for milk/milk products and draft power has been the tradition of ancient India. Majority of the stake holders are from local communities, particularly pastoralists, have been using their traditional knowledge system for rearing the native breeds. The systematic breeding of cows over the centuries has led to the development of as many as 37 cattle breeds in India. Breeds like Sahiwal, Red Sindhi, Gir, Kankrej, Tharparkar and Rathi are mostly reared for milk production. The number of the animals of majority of indigenous breeds has declined over the years attributed to crossbreeding. Further, their number is very low and majority of cattle population (80%) in India is non-descript. However, considering the importance of indigenous cattle, some of the breed improvement programs have been initiated to conserve and multiply these breeds.

Abstract

Local breeds of livestock represent embodiment of evolutionary wisdom fully in tune with ecosystem and able to produce and reproduce in spite of the prevalent hardships and shortages. Indigenous breeds survive and produce where others fail. Each of the recognized 144 indigenous breeds of livestock (covering cattle, sheep, yak etc.) has its unique qualities - some known and some yet unknown - but central to all features is ‘efficiency of production’ by local breeds. A breed in its own habitat makes the most efficient production system; under the same prevalent input system, any other breed from outside would not perform as efficiently. Another valuable common feature of the local breeds is their sustainability of survival and production. Production level may be medium as the animal has to spend energy in order to gain adaptability to high ambient heat, tolerance to drought, resistance to the prevalent diseases and be able to retain capability to digest low quality feed fodder which itself is not sufficiently available. In spite of such hardships, the local breed continues to survive and successfully procreates to sustainably maintain the production system over generations and thereby providing livelihoods to marginal people leading to inclusive growth. Innate features of sustainability of the local breeds provide sustainability to local livelihoods as well. It is noted that the livestock in its entirety, but especially the indigenous breeds proper, have the multifarious capability to provide livelihoods to our rural masses, infuse sustainability to animal-agriculture, and facilitate inclusive development by targeting and supporting the marginal people in the country.

Abstract

In the present study, the village chicken rearing practices were documented in Kannur and Kozhikode districts of Kerala. A total of 128 native chicken farming households were surveyed. The system of management is extensive backyard type with the provision of shelter at night. Around 90% of the farmers had more than five years of continuous experience in village chicken farming. Majority (73.4%) of chicken present in this region was either reproduced within the household from earlier generations or added from the neighbourhoods of the same Panchayat. Most of the farmers (68.8%) consider that chickens are reared for both egg and meat; while, others (31.3%) deem that they are meant for eggs alone. As many as 87 % of the farmers cull the surplus males before one and half years of age. In contrast, majority of farmers (78.1%) do not cull female birds and usually maintain them till their death.

Abstract

The Arabian camels are well known for their existence and adaptation to the desert geo-climatic situations. Their service to mankind in the difficult terrains of the earth for transport and baggage is also well documented in the literature and has clear reflection in society through art and culture. The existence and adaptation of single humped camels in hilly terrains of India is not very much known, but there exists a population of camel in the hills of erstwhile Mewar state of southern Rajasthan and adjoining area. Based on the locality these camels are known as Mewari camels and are well adapted to travel and carry loads across hills. Phenotypically the Mewari camels are stouter and off-white to light brown in colour. Molecular characterization also distinguishes it from other major breeds of camel. The Mewari camels are mainly reared for milk production. In recent study, it was found that of the total camel population with camel milk producers, about 31% are in-milk female camels. On an average about 2.8 litres of milk per milking female is being sold in the market everyday. Assuming that the camel population in the tract has gone down by 25% of its level of the year 2007 and the current survey figures are utilized for extrapolation, the population of camels in the breeding tract comes to 10408 with 4335 breedable females and 3226 female camels in milk. Accordingly, thousand lactating camels are producing 2800 litres of milk per day or 1022 tonnes per annum and are exclusively meeting the milk requirement of 10,000 persons at present level of per capita availability of milk in the country i.e. 276 gm per day. If the production potential of superior animals of the breed is utilized, the per day production from a female camel can be gradually increased to 6 litres or even more as the the best animals produce an average yield of 10 litres of milk per day during a lactation period of 16 months. Thus with 1000 she camels presently meeting the milk requirement of 10,000 people, may meet the milk requirement of 20,000 people by selecive breeeding. With proper policy support, if more she camels can be covered, the population covered can be increased proportionately. The increase in milk production is expected to increase per capita milk availability in the deficient districts and thus may assist us in ensuring nutritional security of the people of the region. Consequently, the increased milk production may further ensure better returns to the camel owners and thus strengthening the livelihood and conserving the camel populations in situ.

Abstract

Camelids across the world are reared for milk, meat, draught, race and tourism. Camelids are also the source of world’s finest natural fibre. They are distributed in the South American, African, Arabian and Asian countries. Apart from this a feral population exists in Australia and recent reports indicate that a few animals of the species are also being reared in Europe. Camel (Camelus dromedarius) is the predominant species of the camelids. The world camel population shows an increasing trend with 12.92 m in 1961 to 26.63 m in 2011 producing 0.42 m tonnes of meat and 2.11 m tonnes of milk per annum. The top 20 camel rearing countries produce both milk and meat with the exception of India and Pakistan as per FAO data. There are about 83 populations of the dromedary camels in the world spread mainly across African and Asian countries. There are about 3.04 m Alpacas in world with Huacaya and Suri as the two main populations and 3.24 m Llamas with Chaku and Kara as the two main populations. The Guanaco population in the world is about 0.5 m and that of Vicuna is 0.35 m. The wild Bactrian camels are around a thousand but the domesticated Bactrian camels are about 0.6 m. The Alpaca and Bactrian camel genome has been sequenced whereas the expressed sequence tags of the Dromedary are available in the public domain. A brief account of evolution, present status and conservation of camelids is discussed.

Abstract

Determining the optimum stocking rate for a grazing land is a key decision affecting the profitability and viability of a grazing system. Forage intake and subsequent performance is dependent on forage availability to the livestock on a daily basis.
Fixing stocking rate too low results in wastage of forage and loss in profit and too high results in lowered forage intake and livestock output thus results in diminished profits. Overgrazing occurs when the grazing pres-sure exceeds the carrying capacity of the land and would modify the physical properties of soil and ecosystem. Due to the overuse of the grazing land, vegetation cover declines and thus reduces the soil organic matter content and soil infiltration capacity. On farm field experiments were conducted in three farmer’s fields at dryland tracts of Tirupur district for a period of two years from September, 2009 to March, 2011 with the objective to assess the carrying capacity of silvipastoral farming system. Treatments were five silvipastoral systems viz.,Acacia leucophloea + Cenchrus ciliaris, Acacia leucophloea + Cenchrus ciliaris + Stylosanthes hamata, Acacia leucophloea + Cenchrus setigerus + Stylosanthes hamata, Acacia leucophloea + fodder sorghum + Pillipesara and Acacia leucophloea + Cenchrus setigerus + Stylosanthes hamata & fodder sorghum + Pillipesara. One unit of Mecheri sheep of five ewes (female) and one ram (male) and two buffaloes were maintained in each location. Observations on carrying capacity, annual forage yield, utilization rate, average daily intake and average fodder requirement of sheep and Length of grazing season were made. The experimental results revealed that, among the different silvipastoral systems, rotational grazing of 39 numbers of sheep per ha of silvipasture land with Cenchrus setigerus + Stylosanthes hamata & fodder sorghum + Pillipesara system is found to be the best. This would imply relatively less pressure on land that would indicate sustaining the carrying capacity of grazing land without exerting pressure on natural resources.

Abstract

Indigenous chickens play vital role in sustainable rural development by providing livelihood and nutritional security to the rural and tribal people. However, indigenous chickens in the country increasingly face the threat of their replacement or erosion in their genetic diversity due to introduction of exotic or improved chickens to enhance productivity of backyard poultry production. Therefore, it becomes imperative to improve the growth and productivity of indigenous chickens in order to make the rearing of indigenous chickens economically attractive proposition. Aseel is one of the important indigenous breeds of chicken that is being conserved and improved for growth and egg production at Directorate of Poultry Research (DPR), Hyderabad. The averageegg production of improved Aseel chickens recorded in a laying cycle of 72 weeks of age was 150.3±5.63with an average egg weight of 48.66 ±0.34g. The average body weights of male and female birds recorded at 72 weeks of age were 3156 ± 27.4 and 2133±36.1 g, respectively while corresponding shank lengths were 135.23±0.7 and 104.9±0.4 mm. Another indigenous chicken germplasm, Ghagus-ecotype was collected from border area of Karnataka and Andhra Pradesh at Kolar district and is being characterized and conserved at DPR. The comparative growth performance of these indigenous chickens was also studied under similar management conditions. It was observed that the growth and egg production of improved Aseel breed was significantly higher than the Ghagus-ecotype chickens. The improved Aseel breed with its better growth and production performance appears to be a promising indigenous chicken for sustainable rural poultry production.

Abstract

Pollinators provide key services to both natural and agricultural ecosystems. Agricultural productivity depends, in part, on pollinator populations from adjacent semi natural habitats. Honey bees are the primary pollinators of mustard crop because it is highly attractive to bees and provides both nectar and pollen. Studies were conducted to assess the diversity and relative abundance of pollinators in mustard (Brassica juncea) of 2011-2012 at Apiary Garden, G.B Pant University of Agriculture & Technology, Pantnagar.A total of 19 insect visitors belonging to order Hymenoptera (15) and Diptera (4) were found to visit the mustard blossoms. Hymenopteran visitors belonged to six families namely Apidae (6), Scollidae (3), Xylocopidae (1), Halictidae (2), Magachilidae (1), Anthophoridae (1) and Sphecidae (1). Lepidopteran visitors belonged to families Pieridae (1). Besides this some Dipteran visitor belonged to families these were Syrphidae (3) and Muscidae (1) were observed on mustard flowers. From the family Apidae, honeybees (Apis mellifera, Apis dorsata, Apis cerana indica and Apis florea), Ceratina sexmaculata, Xylocopa iridipennis and stingless bee Tetragonula laeviceps family Apidae. Scolia (Discolia) binolata, Campsomeriella collaris and C. annulata of family Scollidae, the Alkali Bees (Nomiasp.) and Halictussp. of Halictidae, Leaf cutter bee (Megachile disjuncta) of Megachilidae, the digger bee (Anthophorasp.) of Anthophoridae and Sphex sp. of Sphecidae. Three species from family Syrphidae (Syrphus corollae, Episyrphus valtiatus and Eristalis tenax) and one species from Muscidae (Musca domestica) visited the mustard flowers. The abundance (percentage of insect/m2/2min.) of Hymenopterans were maximum (79.97 %) followed by the Dipterans (16.15 %) and others (3.73 %). In Hymenopterans, the honeybees (Apis bees) were observed maximum (57.55 %) followed by non Apis bees (21.06 %) and the scolid wasp (1.35 %).

Abstract

Irrigation tanks (Traditional water harvesting structures) are our heritage handed over to us by our ancestors and are the lifelines of villages. Tanks are small irrigation structures predominantly serve small and marginal farming communities to sustain the agriculture production by supplementing the monsoon rains. There will be festivities in the villages if its irrigation tanks get filled. A proper maintenance of the tanks will prevent famine, starvation and unemployment and bring in prosperity. In most of the Southern states in India, the major threats to tanks are mainly from the encroachers, inefficiency in the functioning of tank system and improper use by the government itself. Tank irrigation in Andhra Pradesh has played a vital role in the development of its agricultural economy. There are around 79,000 small and minor irrigation tanks providing water for irrigation and domestic use, supporting grazing land, fuel wood growth and ground water recharge. Many of these tanks are hydrologically linked in chains (called tank cascades) that cut across administrative boundaries. With deteriorating tank systems, marginal and small farmers are increasingly at the mercy of monsoon and are forced into a cycle of deprivation and debt. These ancient irrigation systems still function as a crucial element in supplying water for agriculture, and they constitute one of the richest sources of wetland biodiversity in the country. A tank system in a cascade has a small reservoir catchment, the reservoir, a strip of trees downstream of the reservoir that act as wind-breaking barrier, paddy fields, and the village. The village tanks play significant role in food production sector and provide employment in the rural sector. In addition to providing irrigation water, the tanks made the micro-climate pleasant and cool, provided a rich biodiversity and agro-biodiversity

Abstract

Agrobiodiversity is the back bone playing a significant role in food security and sustainable livelihoods. Agricultural systems of India are home to a great diversity of plant species and genetic varieties, but unfortunately these traditional farming systems are facing the danger of extinction today. The present study was conducted from three tribal mandals namely Araku, Paderu and Ananthagiri in Visakhapatnam district of Andhra Pradesh as it is noted as a hub of biodiversity in North coastal zone of Andhra Pradesh. Data was collected by direct interview method and the traditional storage practices for the conservation of agrobiodiversity (landraces) were described.

Abstract

The human race has been dependent on plants for both material and financial needs for millions of years. This has enabled them to evolve a unique system of knowledge on the utilization and conservation of plant genetic resources. Tribals rich traditional knowledge is associated with biodiversity. This knowledge has been acquired over years, treasured and are endowed with and passed on by the local communities and the tribals. Farmers with their age old experience in farming developed a comprehensive knowledge on nutrient content and medicinal properties of various plants. Cereals, small millets, pulses, oilseeds & many more forest plants form important component of food source for the tribal people of North coastal zone of Andhra Pradesh. This knowledge was documented from older generation who could provide age old information about the landraces and traditional life styles which are under threat. This paper briefly describes the extent of knowledge possessed by tribal respondents on Agrobiodiversity in terms of crops and landraces cultivated in selected mandals of Visakhapatnam district. Results indicated low knowledge on landrace of Jowar whereas medium knowledge in case of oilseeds and millets and high knowledge in cereals, pulses and vegetables. This calls for a immediate attention from various stake holders for Agrobiodiversity conservation.

Abstract

High Altitude zone is comprised of 40 mandals distributed in parts of Srikakulam, Vishakhapatnam, East Godavari and Khammam districts of Andhra Pradesh covering a geographical area of 1.8 m. ha. The area of this zone lies between 50 to 1680 MSL and is characterized by high slopes, mountains, hills and hillocks as part of Eastern Ghats. Red soils are the most predominant type (94.8%). A small area is covered under alluvial soils and coastal sands. The mean annual rainfall ranges from 1245 to 1288 mm of which about 70 per cent is contributed by southwest monsoon. Large geographical area (58.9%) in the zone is under forests and the net cropped area is only 19.2% with very little irrigation sources. The tribal people of this area practice shifting cultivation locally known as “podu” cultivation for their subsistence. Rice is the most important crop in the zone occupying 36.2 per cent of the gross cropped area of 0.42 m. ha. The other principal crops grown are millets, mesta, niger and tuber crops. Tea, coffee and other plantation crops are also grown in addition to aromatic and medicinal plants. Cropping intensity is 120 per cent. Forest produce such as honey, gum, soap nuts, tamarind fetch income to the tribal people (www.angrau.net).

Abstract

India is bestowed with immense richness of agro-biodiversity and a rich diversity in landraces / traditional cultivars/farmers varieties in several Agricultural and Horticultural crops. A huge number of crop plants (384) are reported to be cultivated in India. This includes 168 species earlier reported under the Hindustani centre, one of the eight Vavilovian centers of origin and diversity. Further, an enormous richness (326 species) is reported in wild relatives of crop plants. A total of 49 indigenous major and minor crops have been reported in the “History of Agriculture in India”, published by the ICAR. A large number of cereals, millets, oilseeds and vegetable varieties have originated and are cultivated in India, making it one of the richest Centres of Origin of crop and plant diversity in the world.

Abstract

The advent of modern chemical industry has resulted in the release of huge amounts of novel organic compounds, as industrial by-products, pesticides, other agrochemicals etc into the environment. These new compounds tend to resist biodegradation, with potential consequences such as persistence in the environment or bioaccumulation in food chains. Early detection of toxic chemical compounds in the environment, particularly in water, and their biological effects on organisms has therefore become increasingly important. The traditional approach to environmental pollution assessment is based on chemical analytical methods which only provide information about the absolute concentrations of known chemicals in the environmental sample without an adequate interpretation of its toxicity to biota. In order to get more relevant information about environmental pollution risk, it is therefore inevitable to supplement the chemical analytical data with the results of methods providing information on biological impacts. The negative biological effects of pollutants present in all kinds of environmental samples can be assessed using different living organisms or cells as ‘analytical devices’. The biological response following the exposure of living organisms or cells to environmental sample usually gives an information on toxicity, genotoxicity, estrogenicity etc. of the whole mixture of chemical compounds. According to the technical principle, methods of biological monitoring can be classified to bioassays, biosensors, immunoassays, and ecological methods. Besides being sensitive only to the bioavailable fraction of pollutants, biotests also have the power to assess the integrated effect of interacting chemical compounds and to detect the compounds, which are toxic only due to bioactivation.

Abstract

This paper is intended to focus on importance of Agro-diversity, seed conservation practices, impact of monoculture on biodiversity and connected formal and informal institutions and governance mechanisms. Furthermore this paper explores to discuss the innovation processes, technologies which help in conserving existing diversity, process of evolving newer lines that eventually meet the needs of the farems. Open Source Seed Network (OSSN) would be an organisational platform which consists people engaged in conservation, farmers that can develop Value for Cultivation and Use data for the existing crop varieties in different agro- climatic growing conditions using participatory varietal selection. Farmer breeders engaged in selection and development of newer varieties and Farmers institutions involved in production and marketing of seed to other farmers. Further to elaborate the process the stakeholders will perform the responsibility of conservation and revival of the existing varieties, PVS for generating VCU data, PPB, perform Maintenance Breeding, maintain community seed banks, seed production and marketing. In addition we attempts to focus on mechanisms while making public sector as partners in participatory plant breeding which enable development of locally suitable varieties. An access and benefit sharing mechanism based on an open source model rather than on exclusive rights based is planned for free exchange of germplam within the purview of General Public License for plant Germplasm (GPLGP). This paper emphasise advantages of GPL that prevents exclusive rights, bio piracy, safeguarding farmer derived genetic resources, develop legal/institutional framework that eventually allows farmers to freely exchange, save, improve and sell seeds. In the end this could trigger the emergence of an institutional framework in which farmers and plant scientists work together in plant improvement that contribute to a sustainable food system.