eChapters

Introduction

Nutrition is the fundamental pillar of life and an essential foundation of human and national development. Rightfrom the earliest stage of human development, the fetal stage, at birth, infancy, childhood, adolescence, adulthood and old age, nutrition forms the basis for survival, performance, mental and physical development & economic and social development.Nutritional deficiencies during pregnancy, fetal and early postnatal life can have a negative impact not only on the subsequent growth and development but can also lead to changes in metabolic regulation and homeostasis.

Introduction

Nutrition, a basic human need, is a critical part of health and development. Nutrition can be defined as the intake of food, deliberated in relation to the body’s dietary needs. On one hand, good nutrition – an adequate, well balanced diet combined with regular physical activity – is a foundation of good health, whereas,poor nutritionleads toreducedproductivity,impairedmental andphysical development, increased susceptibility to disease, and reducedimmunity. According to WHO, under-nutrition, together with vitamin and mineral deficiencies accounts for one-third of all child deaths and impairs healthy development and lifelong productivity. At the same time, sedentary lifestyles, demographic shifts, urbanization and the liberalization of markets have steered the world population to being overweight along with risk of chronic diseases like hypertension, dyslipidemia, type 2 diabetes, coronaryheart disease, stroke, etc.; thus, resulting in a double burden of malnutrition.

To address this dual burden of malnutrition, individuals needaccess to sufficient and good quality food; but focussing solely on food security is unlikely to solve the problem of global malnutrition. The challenges of malnutrition cannot be solvedjustbyincreasingfoodproductioninordertoimprove healthand nutritional status of the population. To resolve such challenges, awareness about food choices for optimal health plays a pivotal role among population.

Human nutrition is the science of understanding food; its component; intake; and how it is related to health, growth, development and diseases. Human nutrition involves the study of ingestion, digestion, absorption and assimilation of the nutrients; their metabolic functions, deficiency or excess disease as well as their regulation through several factors such as choosing particular food components/nutraceuticals or physical activity.

Introduction

The basic physiological structures of mammary glands in all species are almost same and are located externally in order to fulfill the milk requirement of neonates after parturition. The process of secretion of milk from the mammary glands is known as lacto genesis. Milk provides an almost complete nutrition to neonates from birth till weaning period. It is a complex mixture of various constituents like:

Lipids such a striacyl glycerols(TAG),phospholipids,cholesterols and other sterols, free fatty acids and fat-soluble vitamins

Carbohydrates such as lactose, oligosaccharides

Proteins such a scaseins, α-lactalbumin, β-lactoglobulin, immunoglobulin’s, bovine serum albumin, lactoferrin, lysozyme, enzymes, hormones and growth factors

Non-protein nitrogenous compounds like urea, creatine, creatinine, uric acid and amino acids

Major and minor minerals, water-soluble vitamins and cells (leucocytes, macrophages, and epithelial cells).

Introduction

Proteins are the most abundant organic compounds constituting major part of the body. Chemically, proteins are nitrogen containing macromolecules and polymers of L α-amino acids, which serve a wide range of structural and biological functions in animal body. For example, connective tissue made up of collagen, metabolicenzymes, neuro transmitters,signal in gmolecules, structural components of cell membranes and cell organelles, etc. all are chemically proteins.

Before goinginto the details of protein metabolism, first, we need to understand that there are majorly two sources of proteins in our body: dietary protein and body protein. Dietary protein is taken in diet, while body protein indicates the protein and amino acids synthesized in animal body to fulfill the requirements for growth and development. The digestion of proteins will not be discussed here as it has been described in detail in chapter 12. Dietary protein is digested in presence of several enzymes such as trypsin, pepsin, chymotrypsin, aminopeptidases and carboxypeptidases and is converted into mono-, di- and oligopeptides in stomach and small intestine; and finally converted into amino acids. These amino acids are transported to the systemthrough intestinal mucosa via active transport, from where they enter into the cells for further metabolism. Therefore, ‘metabolismof proteins’ is more appropriatelylearnt as ‘metabolism of amino acids’.

Introduction

The diverse cluster of naturally occurring organic compounds including fats, oils, fatty acids, waxes, phospholipids, eicosanoids, terpenes, steroids and fat soluble vitamins that do not prefer to interact with water but soluble in non-polar organic solvents like ether, chloroform, benzene, acetone, are called as lipids. The main functions of lipids in biological system include storage and transport of metabolic fuel, structural component of cell membranes, insulation & protection of vital organs from shock and carrier of fat soluble vitamins. Contradictory to proteins,lipids are not polymers in nature and are hydrophobic due to presence of a long hydrophobic chain. Apart from this, lipids also act as precursor molecules for several pigments, hormones and signaling molecules.

Introduction

Carbohydrates are the most common source of energy for all living cells, which, in presence of light, are formed by the green plants from CO₂ and water during photosynthesis. It canbe said that ‘light’ is theultimatesourceof energyrequired for all livingcells.Glucose,a monosaccharide,plays a centralroleincarbohydrate metabolismin all plants, animals and manymicroorganisms. Glucose is derived from the polysaccharides (starch, glycogen) and non-carbohydrate sources (amino acids, glycerols, etc.) during metabolism and ultimately converted either into carbondioxide and water orotherintermediateproducts.Complete oxidation of glucose to CO₂ and water provides ~2,840 kJ/mol energy. The other monosaccharides such as fructose, galactose, mannose, etc. are also important molecules in carbohydrate metabolism.

Introduction

Hormones may be defined as specific chemical molecules produced in certain specific organs called specialized cells or endocrine glands. They are delivered into the blood stream or some other body fluid in minute amounts by the circulatory system of the body and their they exerts a physiological effect at a site of action remote from its origin, regulating numerous biological activities including growth, development, reproduction, water & electrolyte balance, storage & usage of energy etc.

Introduction

The basic components of diet include carbohydrate, protein, fat, vitamins and minerals. Vitamins are the essential nutrients which are not formed in our body and required in minute amount from the diet. Vitamins are crucial for the optimal functions of several enzymes; thus important for optimal growth and development. Vitamins perform various functions that help in regulation of metabolism, prevent chronic diseases, maintain natural appetite, mental health and immunity.

In 1912, Polish biochemist Kazimierz Funk coined the term ‘vitamine’;a substance that was present in rice polishing and foods that cured polyneuritis in birds and beriberi in humans. The name is from ‘vit’ for vital nutrient and ‘amine’ for its similarity as an amine. Vital signify that these factors are indispensable for life. Earlier they are identified and thought to contain amino groups, thus the word amine was used, which was true for thiamine but later work revealed that most of the vitamins do not have amino groups. Thus the word was shortened to vitamin in English. It should be noted that the term vitamin does not denote to some othern utrients such as essential amino acids, essential fatty acids, dietary minerals or other nutrients that contribute towards good health.

Vitamins may be defined as organic compound needed in minute amount through diet to perform specific biological functions for normal growth and health of the organisms.Vitamins should have following characteristics:

Introduction

The nucleic acids are the molecular depots that are responsible for continuity of life by transfer of hereditary information from one generation to next. The sequence of nitrogenous bases containing programmed instruction determines the structure of every cell constituent and eventually every protein. In 1869, Friedrich Miescher, Swiss chemist, isolated an unknown phosphate-rich substance from pus cells named as nuclein, which was quite different from other organic substances like carbohydrate, protein, and fat. Altmann in 1899 ascertained that the nuclein also had some acidic characteristics and changed its name to ‘nucleic acid’. Later, nucleic acid was also isolated from salmon sperm nuclei as DNA-protaminecomplex. In 1953, James D.Watson and Francis H.C. Crick constructed the model of three-dimensional double helix structure of the DNA molecule which could successfully explain DNA replication.

Introduction

Billions of chemical reactions are happening inside human body and that too with lightning speed. However, the same reactions if carried out in laboratory, they take a very long time. The reasons behind it are the biocatalysts, also known as enzymes, which are synthesized by living cells. Chemically, they are proteins(except ribonucleic acids: RNA), colloidal in nature, thermo-labile in character and very specific in their action. For example, hydrolysis of proteins by a strong acid at 100°C takes a couple of days; but same proteins can be digested within couple of hours inside human body at 37°C. Similarly, carbonic anhydrase is used as a catalyst by living cells to transfer carbon-di-oxide from cells to blood. This process is 107 times quicker than the same process if occurs without the help of enzymes. Therefore, enzymes can be defined as the biomolecules or biocatalysts which can speed up the rate of the chemical reactions. The substance upon which an enzyme acts is called as substrate and the one which is produced as the result of enzyme-substrate reaction is known as product.

1. Introduction

Sea Buckthorn (Hippophae sp. ), a high altitudinal, thorny, temperate, deciduous, dioecious, actinorhizal shrub or small tree belonging to the Kingdom-Plantae, Clades-Angiosperms, Eudicots and Rosids, Order- Rosales, Family-Elaeagnaceae and native to Eurasean Countries like India, China, Russia, Nepal, Pakistan, etc. (Barik et al., 2016). SBT is having tolerance towards a wide group of pests and insects, can grow in extreme nutrient deficient conditions and temperature range of -40 °C to 40 °C. It contains more than 190 reported invaluable phytochemicals and has the potential for multidimensional use as food, fodder, medicine, fuel, and conservation of soil and bio diversity (Qinxiao and Hongyan, 2003; Barik et al., 2016). SBT berries, leaves, stem and root barks also contain nutrients and bioactive compounds which mainly include lipids, sugars, amino acids, organic acids, fatty acids, carotenoids (β-carotenes, lycopene, zeaxanthin), tocopherols (α β, γ, δ), sterols (ergosterol, campesterol), Vitamins (A, C, E and K), phenolic and flavonoid compounds (gallic acid, caeffeic acid, ferulic acid, isorhamnetin, kaemferol, quercetin, myricetin) and isoprenols (Tiitinen et al., 2005; Anderson et al., 2009; Barik et al., 2016). All these studies are primarily based on the prominent SBT species Hippophae rhamnoides. However, Sea Buckthorn (Hippophae salicifolia) of North East India is an underutilized non-conventional plant and its potential is not yet fully explored. Very scanty literatures are available on the identification, documentation and preliminary qualitative phytochemical characterization of SBT from North East India (Basistha et al., 2010; Pradhan et al., 2015). But there is no any report on systematic qualitative phytochemical characterization of all the plant parts of SBT from North East India in general and SBT of Arunachal Pradesh in partcular.

1. Introduction

Starch is present in the endosperm of cereal grains which provides the necessary energy during the process of germination and seedling establishment and rice starch is reported to supply 22% of global energy intake in humans (Sun et al, 2014). Starch is a semi-crystalline polymer composed mainly of linear and highly branched polymer viz. amylose and amylopectin respectively. Each polymer is composed of several D-glucose molecules and the difference lies only in the linkages between them. Amylose is a linear chain which is interlinked by α- (1→4) linkages and few branched chains (Kizil et al, 2002). Amylopectin subunits are linked by both-(1→4) and-(1→ 6), with about 95% of the glyosidic bonds being-(1→ 4) linkages and roughly 5% being-(1→ 6). The highly branched nature of amylopectin is attributed to the-(1→ 6) linkage.

1. Introduction

Pineapple (Ananas comosus) is a tropical fruit having good sensorial characteristics such as, mechanical properties, flavor, acidity/sweetness ratio, color; and nutritional. Pineapple is known to be a good source of vitamins such as vitamin C, vitamin B and antioxidants. The free radical scavenging activity of pineapple is found to be much higher than banana and is comparable to that of guava (Alothman et al., 2009). However, it is a perishable cash crop that grows in abundance in a short time. This necessitates protecting the perishable fruit against quality loss during storage and distribution.

Fruit leathers are an economic and convenient substitute for natural fruits with high nutritional components. Leathers are made by removing moisture from wet pulp until the desired cohesive “leathery” composition is obtained. Since it is light and low in moisture it has minimum storage problems and is economical to ship (Moyls, 1981). Therefore, making fruit leather from fresh fruits is an effective way to preserve fruits (Maskan et al., 2002). Furthermore, fruit leather has far fewer calories, less than 100 kcal per serving (Huang and Hsieh, 2005) and have a far greater nutritional value (e. g., especially in terms of energy, minerals, antioxidants and fiber) than the fresh fruits because all nutrients are concentrated. There are large numbers of fruit leather available in the market, such as mango leather, apricot fruit leather, grape leather, berry leather, kiwifruit leather, and jackfruit leather (Torres et al., 2015).

1. Introduction

The culinary banana (Musa ABB) of Assam and North East India (locally known as kachkal) is an important and cheap source of vegetable in the diet of local people. It is considered as one of the potential sources of carbohydrates, starch, polyphenols, micronutrients and functionally important bioactive compounds (Khawas et al., 2016). Native starches have limited use in food application as it has many weakness such as narrow peak viscosity range, poor process tolerance, low shear stress resistance, hydrophilicity, thermal decomposition, high retro gradation, and synersis and cooked starches will formed a weak, cohesive and rubbery paste. These facts motivated the employment of modified starches as important functional ingredients in processed foods in recent years because of their improved functional properties over unmodified starches (Fleche et al., 1985).

To overcome these problems physical. Chemical and enzymatic modification of Starch is done. Chemical modification of starch generally involves esterification, cross linking, etherification or oxidation of the available hydroxyl groups on the β-D-glucopyranosyl units that make up the starch polymer.

1. Introduction

Purple colour passion fruit (Passifora edulis Sims) is a tropical fruit and is well known for its nutritional content and medicinal properties (Patel, 2009). Various type of vitamin E such as α-tocopherol (5,7,8 trimethyl), β-tocopherol (5,8 dimethyl),g-tocopherol (7, 8-dimethyl),d-tocopherol (8-methyl) and α-tocotrienol, b-tocotrienol,g–tocotrierol and d- tocotrienol were quantified in passion fruit as antioxidant molecules with various health benefits (Ramaiya et al., 2013).

Foam mat drying convert thin pulp into stable foam by whipping. Eventually, the whipping process increases surface area, and speed up the drying rates which yield dried foam mat powder of acceptable quality in reduced time span (Kudra and Ratti, 2006). Rheology plays a vital role in food manufacture and marketing nowadays viz., design of handling systems, quality control and evaluation of sensory stimuli of viscosity (Barnes, 2001). It also concerns with how all food materials respond to applied forces and deformations. Basic concepts of stress (force per area) and strain (deformation per length) are keys to all rheological evaluations. Stress(r) is always a measurement of force per unit of surface area and is expressed in units of Pascals (Pa). The direction of the force with respect to the impacted surface area determines the type of stress. Normal stress occurs when the force is directly perpendicular to a surface and can be achieved during tension or compression. Shear stress occurs when the forces act in parallel to a surface. Various food shows different rheological behavior and categorized into solid and liquids stages. It basically means that food varies their characteristic in viscous and elastic behaviors commonly known as viscoelasticity of food, caused by entanglement of long chain molecules with other molecules (Abang, 2010). The dough rheology of various flour has been investigated by various research. Although, there has not been any published literature about dough rheology with incorporated passion fruit powder. To the best of our knowledge, there are no published reports regarding the shear stress properties of passion fruit powder incorporated rice dough.

1. Introduction

Organophosphate (OP) and organocarbamate (OC) pesticides are highly used in modern agriculture due to their low persistence and high insecticidal activity. Although they are relatively less persistent than the organochlorines, they have greater acute toxicity with tolerance level ranging from 1 to 8 μg mL-1 (Mulchandani et al., 2001). The high toxicity of OP and OC neurotoxins and their large use in modern agriculture practices has increased public concern, health risk and the consequent contamination of water and food sources (Buonasera et al., 2010). Development of fast and cost effective analytical tools for onsite monitoring of OP and OC contaminations in food, water and in the environment is highly necessary. An approach toward that has resulted the evolution of acetylcholinesterase (AChE) based biosensors for OP and OC pesticides. Ample literature is available on AChE based biosensors (Zhou et al., 2013). But some intrinsic disadvantages remain that limits its utility and field deployment. The most influencing ones are their inability to sustain in organic solvents and the limited class selectivity that demands alternate bioreceptor for pesticide detection. Given the diversity in mode of action of different classes of pesticide, it is quite challenging to come up with different sensor molecules for each class of pesticides. As a continuous effort towards that goal, attempts have been made to use the Glutathione S –transferses (GSTs; EC 2.5.1. 18) for the purpose. GSTs are a multigenic family of cytosolic proteins with multifunctional biological roles, widely distributed throughout the body and found in the liver, kidney, brain, pancreas, testis, heart, lung, small intestine, skeletal muscles, prostate and spleen (Jancova et al., 2010). The important biological functions those are making GSTs the focus of current research includes its catalytic role in detoxification reaction (Lumjuan et al., 2011) over expressions during phase II metabolism as well as drug resistance associated with anticancer therapies of human and the resistance acquired by certain insects while getting exposure to pesticides (Borah et al., 2016).

1. Introduction

King chilli belongs to the genus Capsicum and species chinense, which is world famous for its heat content. King chilli measures 60 to 85 mm (2. 4 to 3. 3 inches) long and 25 to 30 mm (1. 0 to 1. 2 inches) wide with a red, yellow, orange, or chocolate color. The plant came into limelight when it was announced as the hottest chilli in the world in 2006 (Baruah et al., 2014). The people of the North-eastern India use the fruits of King chilli in different food formulations like flavouring curries due to its high-quality fragrance and pungency and also for various medicinal treatments like rheumatism, arthritis, gastritis, ankylosing spondylitis, digestive diseases (Sarwa et al., 2012) and to reduce chronic congestion (Raktim and Changkija, 2009). It is also used as a bird, animal and insect repellent as well as a biochemical pesticide. The extremely hot or burning sensation of chilli is due to the presence of capsaicinoids found only in Capsicum (Hoffman et al., 1983).

In northeastern India, King chilli has been cultivated in a traditional manner since time immemorial and there exists a considerable amount of genetic variability among the landraces (Raktim and Changkija, 2009). The pharmaceutical applications of capsaicinoids are attributed to their analgesic, antiarthritic, anticancer and antioxidant properties. In fact, capsaicin has been at the centre of intense research for elucidating the basis of its pharmacological properties and exploiting the therapeutic potential (Prasad et al., 2005). It has also been reported that capsaicin inhibits the growth of prostate cancer cells (Mori et al., 2006). The antioxidative capacity of chilli is higher than ginger, garlic, mint and onion (Shobana et al., 2000), which may play an important role in the process of chemoprevention (Yu et al., 2002).

1. Introduction

Okara is a by-product generated during processing soybean into soymilk or tofu. Okara is rich in fibre and protein. It contains most of the carbohydrates, some of the protein and a small portion of the oil from soybeans. Okara contains approximately 50% fibre, 27% protein, and 10% fat on dry basis (Li et al., 2012). About 1. 2 kg of fresh okara is produced from every kilogram of soybean processed for soymilk (Vishwanathan et al., 2011). Fresh okara from soymilk production has about 80% moisture. The protein present in okara has good nutritional quality and superior protein efficiency ratio. Other soy components that are also likely present in okara include isoflavones (genistein and daidzein), lignans, phytosterols, coumestans, saponins, and phytates (Li et al., 2012). The most common method of utilization of okara is in the form of animal feed. Tofu or soymilk processing plants primarily dump okara as landfill or resort to burning. Okara can be utilized as a fertilizer as it contains various nutrients required for growth of plants. It can be used as a fermentation substrate for production of ethanol. Okara can also be used in pet foods as it contains high levels of extenders and protein. Apart from these, okara can be used in a variety of food products. It can be used in various food products. Utilization of okara is still in the initial stage. It can be used as a replacement of wheat flour in baked products. It can also be used in various soups, salads or vegetable preparations.

Resistant starch refers to the portion of starch and starch products that resist digestion as they pass through the gastrointestinal tract. Resistant starch is the fraction of starch which is not hydrolysed to D-glucose in the small intestine within 120 min of being consumed, but which is fermented in the colon. It can be used as a functional ingredient in various food formulations (Fuentes-Zaragoza et al., 2010). RS has desirable physicochemical properties (Fausto et al., 1997) such as swelling, viscosity increase, gel formation and water-binding capacity, making it useful in a variety of foods. It helps in improving the appearance, texture, and mouthfeel of the final product compared to conventional fibres. Resistant starch can act as a prebiotic for gut microflora (Sajilata et al., 2006). It has hypocholesterolemic effects, is hypoglycaemic and prevents gall stone formation.

1. Introduction

Banana is having number of species and belongs to genus Musa of the family Musaceae. Bananas are produced in large quantities in tropical and subtropical areas. World production of Musa in 2003 was estimated at 102 million MT of which about 68% was classified as bananas and 32% as plantains (FAO, 2003). For growing banana need special climate and they are mainly produced in developing countries P (Zhang et al., 2005). The crop is of major importance to the people in the growing. The largest producer of banana is India (27%) and other countries are Ecuador, Brazil, Indonesia, Philippines and China etc. Ecuador (42. 3%), Colombia (24. 7%), Philippines, Dominican Republic, Belgium, Italy, Peru, France, Netherland, Germany are the top exporter countries in 2009.

Banana is a botanically a berry require a warm, humid, frost-free climate with optimum temperatures between 22 and 31°C. In several report it was found that banana plant die in ground level at below temperature -2°C. Primary component of the aroma of fresh bananas is isoamyl acetate which is also called as banana oil. Banana is highly energetic food and rich in potassium, sodium, phosphorus, chlorine, magnesium, sulphur, silicon, calcium; and the vitamins: A, B1, B2, C and B3 (niacin). Banana helps in lowering cholesterol, enhances liver function, regulate heart rhythm and disease, decrease the risk of stroke, regulate Bowel movement, helps keep blood pressure under control and helps in weight loss.

1. Introduction

Jackfruit (Artocarpus heterophyllus Lam. ) a member of the family Moraceae is the largest tree-borne fruit in the world. India is the second biggest producer of the fruit in the world and is considered as the motherland of jackfruit. The jackfruit is native to parts of South and Southeast Asia and is believed to have originated in the rainforests of Western Ghats of India and is cultivated throughout the low lands in South and Southeast Asia. Major jackfruit producing countries are Bangladesh, India, Myanmar, Nepal, Thailand, Vietnam, China, the Philippines, Indonesia, Malaysia and Sri Lanka. There are 100-500 seeds in a single fruit (Sidhu, 2012). It is composed of rind, edible bulbs of yellow-flesh and seeds. The ripe fruits are eaten or processed into snacks and canned products. Seeds make up around 10 to 15% of the total fruit weight (30-365/ fruit) and have high carbohydrate and protein contents (Ocloo et al., 2010). Mostly the seeds, rind and strands are discarded as waste. So, the seed, rind and strand flour can be an alternative intermediatory product, which can be stored and utilized, both for value addition and to blend with other grain flours without affecting the functional and sensory profile of the final product.