eChapters

A. Answer the following.

Q.1.     What is the definition of sequestered antigens ?
Ans.     Sequestered antigens are antigens which are protected from the immune system and no immune response is generated against them. Ex lens and uveal proteins of the eye, chondrocyte antigens in cartilage and antigens of spermatozoa.

Q.2.     What is autoimmune haemolytic anaemia ?
Ans.    It is an antibody mediated autoimmune disease and antibodies are generated against self RBCs. It occurs in unmatched erythrocyte antigen in transfusion or in erythroblastosis fetalis. The antibodies developed in autoimmune haemolytic anaemia are of two types (1) warm autoantibodies (2) cold agglutinins. The warm

Genus : Garcinia
Species : mangostana L.
Family : Guttiferae
Chromosome No. : 2n = 56-76, 88-96, 120-130

In family Guttiferae to which mangosteen belongs, very few plants are fruits and yield drugs, gums and resins. It is a tropical fruit and occupies an important place in Garcinia family. The European voyagers on their return indicated wonderful stories of the East and mention of mangosteen received huge praise. Hacobus Bontius termed the fruit as ‘Queen of fruits’ the finest fruit in the world. Because of beautiful colouring of fruit alongwith pleasant delicate flavour makes mangosteen the most esteemed fruit of Asiatic tropics and no other tropical fruit equals to that of mangosteen. Despite being designated as the finest fruit of the tropics, the fruit is mainly confined to the limits of East Indies. The species is found wild in the forests of Sunda Islands and of the Malay peninsula. Writers have indicated that mangosteen is usual dooryard tree in the East Indies, more in Java and Sumatra. Some small orchards of mangosteen have come up in Sri Lanka, but they are most abundant only in Malay Archipelago.

ABSTRACT

An effort was made to characterize twelve ber genotypes using RAPD markers. Of the twenty random decamer primers tested, 17 generated polymorphic bands, while rest of the primers showed monomorphic bands. The primers that amplified all the 12 genotypes were OPC-20, OPD-1, OPD-3 and OPD-4. The size of amplified DNA fragments varied from 0.56-4.26 kilobase pair. In total, 109 bands were obtained, of which 85 bands were polymorphic, while 24 bands were monomorphic. For the cultivars tested between 2-9 bands were obtained for each primer with an average of 5.45 bands per primer. The highest number of bands (9) were generated by OPA-4 and OPD-3. With un-weighted group method using arithmetic mean (UPGMA) cluster analysis, the twelve ber genotypes fell into four major clusters. The pair wise dissimilarities of ber genotypes showed that genotypes G-9 and G-8 were close to each other. Maximum dissimilarity was observed between the genotypes G-8 and G-12.

Regulator Genes

Bacterial cells are capable of producing many kinds of enzymes in relatively large quantities but the kinds and amount of enzymes actually produced at any given time are usually those which are required at that particular time in response to the prevailing environment. Inducible enzymes are ordinarily absent or present in very small quantities until a specific compound appears. This compound is called as inducer. A typical example of this is production of b galactosidase (an enzyme that splits lactose into galactose and glucose, Fig. 44.1) once the lactose is introduced into the medium on which E. coli cells are grown. Here lactose acts as inducer for the enzyme b galactosidase.

Burn wounds are considered to be one of the most devastating kinds of trauma that affect the physical, physiological and psychological well being of an individual. Based on the depth of the affected area, burn wound is divided into three categories: first degree (superficial), second degree (partial) and third degree (full thickness). Extensive and deep burns result in scar formation and subsequent deformation of the affected part. Healing of burn wound involves several interrelated and overlapping stages such as inflammatory, proliferative and remodeling phases. The interaction of a variety of mediators, cells and extracellular matrix proteins are involved in this three phases of healing.

Burn wound therapy has been performed in various ways such as bioengineered skin grafts and administration of growth factors. Small intestinal sub-mucosa (SIS) is a resorbable xenogenic bioscaffold that has induced constructive remodeling in variety of animal models. It is unique from other acellular matrices as it contains few growth factors such as fibroblast growth factor (FGF) and transforming growth factor (TGF) that play a role in the regenerative process. Chitosan is currently proposed to be one of the most promising polymers in wound dressing development and it accelerates wound healing. Epidermal growth factor is known to stimulate growth of keratinocytes in vitro, thus it might be expected to promote wound healing.

In this chapter efficacy of Chitosan and porcine acellular small intestinal sub-mucosal (SIS) matrix seeded with bone marrow derived mesenchymal stem cells (BM-MSCs) and recombinant murine epidermal growth factor (m-EGF) on healing of full thickness burn wound in rat model is described.

ABSTRACT

Nowadays it is very important to evaluate research publications. They are evaluated by impact factors and citation indices. The research value of an author and journal is ascertained from several citation indices, from which it is known that what Impact of a research publication is in market. The h index is based on diagnostic importance or productivity so it confers a delegate index of scientific result. The h-index is used to measure of published work of a scientist or scholar as well as also assess the achievement of research groups. The present paper describe about the h-index. Specifically, it explains its origin, advantages, and disadvantages and how calculates individual’s h-index in different databases.

Keywords: Citation, Impact, H-Index, Research productivity, Scientific publications.

Parametric and Non-parametric tests

Parametric and Non-parametric tests are used for comparing differences between groups (e.g. Difference in catch-per-unit-effort (CPUE) of two types of Codends) and to test the relationship between two or more variables (e.g. Gender differences in awareness levels of fish farming (High, medium and low). As there seen differences in types of data, Non-parametric statistics are used when

• Data is non-normal

• Small sample size

• The variables are categorical

• Short response categories (<5 ) in scales

Introduction

Planning scripts for documentary production and drama is crucial for creating compelling and effective visual narratives. Documentaries focus on presenting factual content and real-life stories, aiming to inform or inspire audiences through a detailed exploration of a specific topic. In contrast, dramas revolve around fictional stories designed to evoke emotions, explore complex characters, and engage viewers with imaginative scenarios. Both genres require meticulous planning and scripting to ensure clarity, coherence, and impact. For documentary production, the script serves as a blueprint that guides the storytelling process, balancing factual accuracy with engaging presentation. In drama production, the script outlines the narrative structure, character development, and dialogue to create an immersive and emotionally resonant experience. Understanding the nuances of scripting for both types of productions allows creators to effectively communicate their messages and captivate their audiences.

There can be no scientific studies which do not involve the process of measuring. Measurement process is the integral part of research activity. Measurement can be defined as the assignment of numerals to the objects or events according to set of rules.  The emphasis in this definition is on phrase ‘set of rules’. If the rules can be set up based on some rational or empirical basis, then theoretically anything can be measured. It is very much easy to understand the concept of measurement in case of physical sciences (Example weight, length, mass etc.) but it is harder understand the concept of measurement in case of psychological objects/phenomenon (intelligence, aggressiveness, attitude etc.) without understanding the predetermined rules of measurement. A rule is a guide, command that tells you what to do/how to do.

Taylorella was first isolated by Taylor et al. (1978) as a causative agent of contagious equine metritis (CEM), a venereal disease of horses. The disease (CEM) was first reported in the mares of thoroughbred horses in UK in 1977 breeding season (Crowhurst, 1977). Initially the causative agent was assumed as Haemophilus equigenitalis. Later on the basis of numerical taxonomic characteristics, genomic G+C contents, DNA-DNA hybridisation studies, the new genus Taylorella was proposed in 1983 (Sugimoto et al., 1983). It was approved by the International Committee on Systematic Bacteriology.

Properties of genus

1.   Morphology :  Taylorella are gram negative, small rods or coccobacilli, measuring 5-6 µm by 0.8 µm. They are pleomorphic, piliated, encapsulated, non-sporing and non-motile.

2.   They are microaerophilic (require low O₂ tension for growth), non-fermentative, and non-proteolytic in nature.

3.         Classification : Taylorella belongs to β- subclass of class Proteobacteria (whereas, Haemophilus belongs to the γ- subclass of Proteobacteria). Major important species is Taylorella equigenitalis. The genus was considered as a ‘single species genus’ for a long time. Latter in   1997, National Veterinary Services Laboratories confirmed the identity of another Taylorella species, isolated from a donkey jack (Equus asinus) in California. It was named as T. asinigenitalis. The new species has minor difference in 16S rDNA sequence (97.6% similarity), DNA–DNA hybridization (23% similarity) and (G + C) composition with T. equigenitalis (Matsuda and Moore, 2003)
.

ABSTRACT

The discovery of polymerase chain reaction method of amplification of DNA is an important milestone in molecular genetics and used for multiple purpose such as construction of linkage maps, marker saturation of specific genome regions, analysis of genetic diversity, molecular phylogeny and cultivar identifications (Williams et al; 1990, Demeke, Adams and Chibbar; 1992, Welsh and Maclelland; 1990;, Obara-Okeyo and Kako; 1998, Sawhoon Lin et al; 1998 and Grunger et al; 1998.). 15 species of three morphologically distinct groups of Orchids belonging to the subfamily Epidendroideae and tribe Dendroebieae and Cymbideae were studied using random amplified polymorphic DNA (RAPD) technique. Genetic variability and relation between these 3 genera and 15 species were estimated based on band sharing and cluster analysis. A total of 227 distinct major RAPD bands of which 97% were polymorphic were generated from 15 arbitrary primers. The number of bands generated per primer ranged from 3 (Primer A-10) to 22 (Primer K-10 and N-17) with a mean of 14.8 bands per primer. Out of 227 bands generated 222 bands (97.8%) where polymorphic while only 5 bands were monomorphic. The primer resolving power was found to range between 2.00 (prime A-10) and 13.38 (primer k-10) when all the species were studied were taken into account. The molecular analysis grouped all the species into 5 groups. The polymorphic patterns generated by RAPD profiles showed different degrees of genetic relationship among the species studied and the RAPD marker were found to be an useful tool for detecting genetic variation within the species of three important genera of Orchids.

5.1 String Statement

String in python is stored as individual characters i.e. indexed wise. The index by default starts with 0.

So, here P will be represented by str[0] or str[-6], y will be represented by str[1] or str[-5]

Note: strings are immutable and hence you cannot change the individual letters of string using assignment operator.

Example:

name=“shyam”

Name[0]=“A”

Result error

Male sterility is a condition in plants whereby the anthers or pollen are nonfunctional. The condition may manifest most commonly as absence of pollen or extreme scarcity of pollen, severe malformation or absence of flowers or stamens, or failure of pollen to dehisce. Just like self-incompatibility, male sterility enforces cross-pollination. Similarly, it can be exploited as a tool to eliminate the need for emasculation and for hybrid seed production on commercial scale in field and vegetable crops. There are three basic kinds of male sterility based on the origin of the abnormality related to functional pollen grain.

Introduction

Black cotton soils are formed by the solidification of molten magma; it covers a large area of the Deccan plateau. The depth of this soil is very high in the lower part of the Krishna – Godavari basin. The clay fraction is very high which is responsible for the heavy waterlogging in the area of heavy rainfall and become more vulnerable to soil erosion. The fine fraction of soil not allows draining out through the soil profile and at the same time the rate of evaporation is low. During summer soils suffer from moisture stress and it becomes very hard but at the same time during the rainy season moisture content very high. this imposes severe constraints on conventional agriculture use as a result most of the land remains fallow and subjected to soil erosion due to heavy rain. The nutrient status of the soil is quite good in Vertisol but these constraints limit crop production. To manage black cotton soil for crop production it is advisable to use animal-powered devices for surface soil drainage, Counter cultivation, adoption of a new cropping system will be helpful for better crop production.

There’s no one-size-fits-all approach to writing a documentary script, but there are best practices that can streamline the process. Here are some key tips for crafting a compelling documentary script:

Choose a Story You’re Passionate About

Start by asking yourself why you want to create this documentary. What message do you want to convey? Why are you the best person to tell this story? These are questions that producers and executive producers will expect you to answer in a pitch, but it’s crucial to first address them for yourself. The answers will guide you to a story that you’re genuinely passionate about, making the long and challenging process of documentary filmmaking worthwhile. Once you have clarity on these questions, you can dive into the task of researching your material, identifying the story, and crafting the script.

Ultrasonographic images of stomach are required to diagnose ingestion of any abanormal materials by dog. When content of stomach are dry or there is gas in stomach then, it is difficult to get proper image of stomach. Offering water to animal or oral administration of water with the help of syring is also helpful in getting the images of content of stomach. Some time, while doing ultrasonography, movement of contents of stomach can be visualized. Stomach can be images between liver and spleen. The size depends upon contents or intake of food by dog. For proper image of gastrointestinal tract including stomach, there should not be gas in GIT. One should remember that ultrasound beam does not travel in gas. The best medium of ultrasound beam is liquid.

Scanning Technique

If possible, fasting for 12 hours is good to diminish the amount of gastrointestinal gas. However, non-fasted dogs, when offered water may show adequate image quality. The water may be administered in quantity of 15 ml per kg of bodyweight. Removing the stomach gas using an orogastric tube has been recommended tto enhance the visualization of lesions of the stomach, however, this is not in practice in India.

Advertising has long been a powerful tool for businesses to communicate with their target audience, influence consumer behavior, and build brand awareness. While digital marketing has gained prominence in recent years, traditional advertising remains a fundamental part of marketing strategies. Traditional advertising encompasses a range of media channels such as television, radio, newspapers, magazines, billboards, and direct mail. This chapter explores the various aspects of traditional advertising, its effectiveness, advantages, and challenges in the modern marketing landscape.

Evolution of Traditional Advertising

Traditional advertising has evolved significantly over the years. In the early days, businesses relied on word-of-mouth, signage, and posters to promote their products and services. With the advent of mass media, advertising took new forms

• Print Advertising: One of the oldest forms of advertising, print media includes newspapers, magazines, brochures, and flyers. Businesses have historically used print ads to reach a broad audience

Introduction
Parasitism is a biological interaction in which one organism, the parasite, lives on or within another organism, the host, causing harm. In the context of plant pathology, parasitism is a crucial concept, as many plant diseases are caused by various parasitic organisms, including fungi, bacteria, viruses, nematodes, and protozoa. Understanding the mechanisms of parasitism and pathogenesis is essential for developing effective strategies to manage plant diseases.
Definition & Concept of Parasitism
Parasitism is defined as a symbiotic relationship where one organism benefits at the expense of another. In plant pathology, this interaction typically involves pathogenic organisms that exploit host plants for nutrients, ultimately leading to disease.
Types of Parasites
Plant pathogens are generally categorized based on their nutritional relationships with their host plants. These relationships determine how the
pathogens interact with the host and whether they can survive outside the host environment. There are two primary types of plant parasites:

The complex interplay of biotic, chemical, and physical forces that shape an organism’s structure and survival within an ecological community is known as its environment. The “backdrop” to the story of human history is the environment, which includes the “wilderness” that humans have not yet domesticated or subjugated, the “hinterland” that surrounds human settlements, and the habitats and resources that humans exploit.
Everything that exists naturally on Earth or in a particular place, both living and nonliving, is included in the natural environment. The lithosphere, crust, upper and lower mantles, inner and outer cores, and other layers make up the structure of the earth.
Every living thing, including non-living ones, interacts with every other thing constantly in its surroundings. People in particular need an environment to survive. Humanity and the environment are mutually dependent. The state of the environment is greatly impacted by human activity, and this has an effect on how our civilization is developing as a whole.