eChapters

Introduction

Seafood undergoes rapid spoilage after capture or harvest due to their highly perishable nature. Spoilage refers to any change that renders seafood unacceptable for human consumption. Spoilage occurs as a consequence of various microbial, biochemical and chemical breakdown processes. Spoilage leads to quality changes in seafood. Quality change refers to any change that occur after harvest due to unpredictable catch, uncontrolled harvest, inadequate handling, processing and storage facilities. The initial quality loss is mainly due to the post-mortem autolytic activity (enzymatic process) and chemical degradation process (lipid oxidation); and later by microbial degradation. The quality problems occur as a result of poor-quality raw material, ice and water, microbiological contamination, presence of heavy metals and other storage aspects during processing.

Processing of fish means preparing raw fish into a marketable form. Pre processing is done prior to final processing. Pre-processing is often done on board fishing vessel or in a shore based plant. It includes operation such as washing, sorting, grading, butchering, etc of the harvested fish. The butchering involves removal of non-edible portions such as viscera, head, tails and fins from fish. The yield of fish meat varies from 30 to 70% depending on the butchering process. Butchering process includes beheading, gutting, filleting, peeling, deveining, shucking, etc. The discarded portion of fish meat are fish wastes, often used for animal feed or for conversion to bioactive functional foods. Final processing of fish includes processes such as heating, freezing, drying, salting, marinading, irradiation, or addition of chemicals. The processed seafood is categorized into five major groups – chilled, frozen, canned, cured and other value-added products in this chapter.

56.1. Aim

To determine quality characteristics of dried and dehydrated fruits and vegetables.

56.2. Theory

The quality of dried/dehydrated products can be measured by estimating moisture contents, preservatives level, acid insoluble ash contents and most importantly enzyme reactions.

56.3. Estimation of Acid insoluble ash

1. To total ash add 25 ml of dilute HCl (10% w/w)

2. Cover with a watch glass and boil gently over a low flame for 5 minutes

3. Filter and wash the filter paper thoroughly with hot water

4. Return the paper to the original dish, ignite, cool and weigh the insoluble ash

Objective: To check quality of genomic DNA on agarose gel electrophoresis.

Background information

Agarose gel electrophoresis is a common method of gel based electrophoresis technique used in biochemistry, molecular biology, genetics and clinical chemistry for separation of Protein and nucleic acids from a mixture of macromolecules. Agarose is a major component of agar that is used as a matrix in gel baseelectrophoresis. The nucleic acids (DNA and RNA) are separated based on length of fragments while, proteins are separated based on size and/or charge (Kryndushkin, et al. 2003). For separation of bio-molecules the electric field is applied that leads to movement of charged particles via agarose gel matrix and bio-molecul s are separated based on difference in size in the agarose gel matrix (Sambrook and Russel, 2001).

Gel casting with agarose is easy and has very few charged groups, hence found most suitable for nucleic acid separation in most of the biochemistry, molecular biology and biotechnology laboratories. Separated DNA/RNA ma be seen and recorded with stain under UV light. Further DNA fragments can be eluted from agarose gel with an ease for further down applications. The percentage of agarose gel used is in the range of 0.7 - 2. Lower percent of agarose gel is used for long DNA fragments while, higher percent of agarose gel is used for short DNA fragments with suitable running buffer.

Objective: To check the integrity of isolated RNA on agarose gel.

Background information:

The most common method used to assess the integrity of total RNA is to run RNA sample on agarose gel (denaturing) stained with ethidium bromide (EtBr). While native (non-denaturing) gels can be used, the results can be difficult to interpret. The secondary structure of RNA alters its migration pattern in nativegels so that it will not migrate according to its true size. Nondenaturing conditions also result in bands that are not as sharp, and even multiple bands representing different structures of a single RNA species.

Intact total RNA run on a denaturing gel will have sharp, clear 28S and 18S rRNA bands (eukaryotic samples). The 28S rRNA band should be approximately twice as intense as the 18S rRNA band. This 2:1 ratio (28S:18S) is a good indication that the RNA is completely intact. Partially degraded RNA will have a smeared appearance, will lack the sharp rRNA bands, or will not exhibit the 2:1 ratio of high quality RNA. Completely degraded RNA will appear as a smear (having very low mw). Inclusion of RNA size markers on the gel will determine the band size or smears size and will also serve as a good control for ensuring proper gel run. Note: Poly selected samples will not contain strong rRNA bands and will appear as a smear from approximately 6 kb to 0.5 kb (resulting from the population of mRNAs, and depending on exposure times and conditions), with the area between 1.5 and 2 kb being the most intense (this smear is sometimes apparent in total RNA samples as well).

Quality control significantly affects manufacturing output, productivity and operational excellence. Techniques such as Six Sigma and DMAIC systematically identify the occurrence and causes of defects while developing solutions to reduce them. Total Quality Management further improves quality and lowers costs associated with defects, wastage and rework. Common tools employed in garment manufacturing include flow charts, check sheets, histograms, Pareto charts, scatter diagrams, control charts and cause-and-effect diagrams. Their systematic application supports continuous process improvement and better economic performance. Because the textile and apparel industry faces intense competition in domestic and global markets, manufacturers must shift from simple product inspection towards integrated, preventive and continually improving quality-management systems.

Despite its existence and continued use over many centuries, and its popularity and extensive use during the last decade, traditional medicine has not been officially recognized in most countries. Consequently, education, training and research in this area have not been accorded due attention and support. The quantity and quality of the safety and efficacy data on traditional medicine are far from sufficient to meet the criteria needed to support its use worldwide. The reasons for the lack of research data are due to not only to health care policies, but also to a lack of adequate or accepted research methodology for evaluating traditional medicine235.

The statement from General Guidelines for Methodologies on Research and Evaluation of Traditional Medicines (World Health Organization, 2000) highlights the need for consensuous in addressing the problems related to the quality of botanical drugs, which ultimately is a critical determinant of safety and efficacy of medicine. Further, the distribution and widespread sale of adulterated, misbranded, spurious botanical drug products and marked increase in misleading health claims of these products demands proper regulations on botanical medicine to protect public health236. Considering these two issues development of parameters for quality control standardization and regulatory norms for controlling the growing business of botanicals is a big task. Various regulatory authorities, research organizations and botanical drug manufacturers are constantly contributing to developing guiding principles addressing issues related to quality, safety and efficacy237. In this chapter we have highlighted problems associated with quality control of the botanicals and tried to guide a way towards a globally acceptable quality standard.

1.introduction

The total sum of foods or mixture of foods which an individual consumes each day is referred to as his diet (Okey, 1992). Vegetables that are used as food has nourishing substances called nutrients namely carbohydrates, proteins, fats, minerals and vitamins. The nutritive value of different vegetables varies sufficiently and the usage of variety of vegetables ensures all the necessary nutrients required in the diet and its lack in daily menu may result in various disease and deficiencies (Saxena, 1970).  They contain a great variety of all other phytochemicals, some of which claimed to have antioxidant, antimicrobial and also anti-carcinogenic properties (Grudha et al., 1996). But when soaking is done in water either plain or salted with sodium chloride, water soluble nutrients may be leached into the water (Srilakshmi, 1999). An attempt was made in the present study to find out the effect of soaking of some selected vegetables in water for one hour after cutting them into pieces and its nutrient loss.

Most raw feed ingredients used as an energy and/or a protein source in poultry feed are grown, harvested, processed, and transported from outside of the poultry industry. Poultry feed are routinely subjected to contamination from diverse sources which may have a serious consequences on the safety of poultry products. Public concerns over food safety from the food of animal origin is a cause of global concern in the recent years due to problems such as bovine spongiform encephalopathy (BSE), dioxin and melamine contaminations, microbial resistance to antibiotics and outbreak of food borne bacterial infections (food poisoning) (Panda, 2013).

Adulteration in animal and human foods has become common feature in the country, therefore we should be cautious while selecting animal/human feed ingredients.

The author of this compendium has developed. “Animal Feed Testing Kit” based on novel cud liquor enzyme complex (innovation certified by ISA, International Search Authority - Patent office, Vienna), registered with WIPO (Geneva). This method is described in detail in this chapter and Patent application is field at Indian Patent Office Delhi.

Now-a-days, animal feed is being adulterated with extraneous materials viz, Saw dust, Ric hulls, sand and Silica, Castor husk, oat hulls and urea (higher than 01 percent limit on dry matter basis).

Quality control in fish feed manufacturing plays a vital role in ensuring the safety, nutritional value, and uniformity of the final product. It involves a structured set of procedures carried out throughout the entire production cycle— from sourcing raw materials to packaging the finished feed—to confirm that the product adheres to standards that support fish health, growth, and environmental stewardship. At the initial stage, raw materials are tested for factors such as moisture, protein, lipid content, fiber, ash, and the absence of harmful elements like aflatoxins, pesticides, or heavy metals. Leading feed manufacturers enforce strict supplier verification and sampling routines to maintain ingredient quality and authenticity. During production, critical parameters are routinely checked, including grind size, mixing consistency, and temperature and moisture levels during conditioning. The physical integrity of pellets is also evaluated during extrusion or pelleting. Specific performance indicators—like water stability, floatability for floating feeds, and feed conversion ratios (FCR)—are assessed to determine feed effectiveness. Post-production checks include examining the pellet’s appearance (size, shape, and color), along with chemical assessments

Quality Control is very important in biofertilizer production, which determines the efficiency of microbial strains. Generally, the quality control based on BIS are voluntary in nature. However, some of the biofertilizers are brought under the ambit of Fertilizer Control Order 1985 (FCO) during 2006 (Yadav and Chandra, 2014). At present, biofertilizers are under the FCO with specific quality standards. Biofertilizer production and its sales can be regulated and is a mandatory requirement for registration of manufacturing unit with the State Fertilizer Controller (who is generally the Commissioner or Director of Agriculture Department) under the statutory provision of FCO. Moreover, some officers of the Agriculture Department have been declared as Fertilizer Inspectors at the district level, who are authorized to inspect production and storage facilities and draw samples for quality analysis. National Centre of Organic Farming (NCOF), Ghaziabad under the Govt. of India and its six Regional Centre’s located at Bhubaneswar, Bangalore, Jabalpur, Nagpur, Hissar and Imphal have been declared as testing laboratories. Under the provisions of the FCO act, State Governments can also develop their own quality control laboratories and notify them under the FCO (1985). Peat, lignite, peat soil, humus, wood charcoal or similar material favouring the growth of microbial strain could also be used. The FCO specifications for different biofertilizers are presented below:

Biofertilizers are one of the important components of organic farming and sustainable agriculture. Awareness among farmers is increasing on the biological fertilizers. But, the success of biofertilizer inoculation depends on the quality of the inoculant used. Because farmers are unable to judge the quality of the inoculants at the time of purchase or even at use. The inoculant producers are negligent to institute quality control programmes. Unfortunately much of the inoculant produced in the world today is relatively of poor quality. In such situation maintaining high quality inoculant products depend on the internal implementation of effective quality control systems by manufacturer. A major goal of a quality standard is to control the quality of inoculant to be used in any country.

Quality control plays a vital role in ensuring food safety and freshness. Inspecting, testing, and grading fruits and vegetables is essential to confirm that food products meet safety standards. In today’s globalized food industry, quality control tests are conducted at various stages throughout the supply chain, from cultivation and harvesting to processing, packaging, transportation, and distribution.
In agriculture, the quality of grain is determined by its intended use. In ethanol production, factors such as starch content are crucial, while in food and feed manufacturing, properties like protein, oil, and sugar are of primary importance. In the milling industry, the soundness of grain is the most significant factor in determining its quality. For grain farmers, high germination percentages and seed dormancy are essential characteristics to monitor. For consumers, visual qualities such as color and flavor are key indicators of quality. Quality control ensures that the grain meets the needs of both producers and consumers while maintaining food safety.
RESOURCES FOR QUALITY CONTROL
PRE-HARVEST TESTING
Pre-harvest tests are essential tools that help growers assess the needs of their crops. These tests assist in making informed decisions regarding nutrition or pesticide applications, determining the optimal harvest windows, and supporting various farm management decisions. By conducting pre-harvest testing, farmers can enhance crop health, improve yields, and ensure that their crops are ready for harvesting at the best possible time.
Starch Iodine Test
Starch is a carbohydrate present in various plants, including apples. As apples ripen, the starch gradually converts into sugar. The amount of starch in an apple

Preservation methods are generally believed to be responsible for a depletion of naturally occurring antioxidants in food. Processed fruit and vegetables are expected to have a lower health protecting capacity than fresh ones. This is because, up to now, only selected and barely stable antioxidants of nutritional interest (e.g. ascorbic acid) have been commonly assessed as indicators of processing damage. On the basis of these supposed negative effects, in the past few years, the main tool for minimizing eventual processing damage was the strategy of ‘reconstitution’, achieved by the addition or the enrichment of the product with natural antioxidants.

It is well known that food processing can have many effects not all of which result in a loss of quality and health properties. Blanching also represents a useful tool in preventing enzymatic oxidations, which are the main cause of loss in naturally occurring antioxidants in raw material of plant origin. In fact, fruit and vegetables subjected to blanching retain most of their original antioxidant properties.

Lycopene appears to be a relatively stable compound during food processing but it has been reported that heating tomato pulp to produce paste, ketchup, juice and other products, can cause degradation of lycopene and other carotenoids. Other antioxidants naturally present in tomato, such as vitamin C, vitamin E, polyphenolic flavonoids and non-lycopene carotenoids, may play a role in preventing the degradation of lycopene. Thermal processes might break down cell walls and weaken the bonding forces between lycopene and the tissue matrix. Such disruptions in the cell wall fraction may enhance the release of phytochemicals from the matrix. This may make lycopene more accessible or more stable and thus increase the nutritional quality and antioxidant activity of product. An increase in carotenoids by thermal processing has also been attributed to enzymatic degradation which causes weakening in protein-carotenoid aggregates. Destruction of vitamin C is destroyed mainly due to oxidation reactions and the heat applied in the presence of air. The degree of loss of ascorbic acid is closely correlated to the drying temperature used for the production of the end product. The increase in the amounts of some phenolic substances having antioxidant activity was associated with two concomitant events: firstly, the thermally induced extraction of antioxidant molecules previously complexed or polymerized and secondly, the retention of active compounds caused by the inactivation of the enzymes involved in their catabolism.

The organic quality is based on standards, inspection, certification and accreditation. All organic food is produced and handled according to strict rules called ‘Organic Standards’. Organic standards do not define a quality status which can be measured in the final products (e.g. quantity of pesticides residues, heavy metals, etc). They define the way of production (e.g. that no chemical pesticides and fertiliser are used).

“Quality Control” constitutes the very backbone of the success in any industry, in any field of activity, may it be agriculture or spices, since quality is never an accident, but, the result of a conscientious effort, as rightly visualized by John Ruskin. The quality of spices is evaluated by the following three types of techniques:
1. Physical or sensory evaluation
2. Proximate physico-chemical composition and determination of chemical constituents
3. Nutritional assessment
In the following table (Table 61) presentation of the five proximate physicochemical compositions of 5 minerals and 5 vitamins in as many as 35 spices is presented. Since most of the spices are used basically in small quantities in both vegetarian and non vegetarian food as “Food Additives”, they contribute directly very little to human nutrition, but, indirectly spices can play a very valuable role in healthy human nutrition by not only helping to increase the appeal and appreciation of the food dishes, but, also in various other ways as agents with nutraceutical and pharmaceutical properties. For instance, Turmeric has been found to have immense pharmaceutical and nutraceutical properties. Most recently, it has been found to be very beneficial during the covid pandemic. Excepting in most restrictive diets, spices are regularly used in most food preparations and they can make a difference which persuades a patient to stay faithful to an otherwise unappealing diet. The analysis confirm that even the highest calorie spice, Poppyseeds, will add no more than 2 or 3 calories per serving in normal usage. This should be good news, in particular, to weight – watchers. Considering the amounts used, the others typically contribute no more than a calorie per serving, and, usually less than that. Having this definitive data at last, nutritionists and all those interested in good nutition may utilize spices to their full potential in making wholesome foods as excitingly delicious as they are good for us.

Quality control comprises the operational activities and techniques used to ensure that products satisfy established quality requirements. While quality assurance concentrates on preventing problems, quality control identifies and corrects defects during manufacturing or service delivery. In textile and apparel production, it covers yarn strength, fabric weight, dyeing accuracy, seam strength, stitch density, garment measurements, fit, finishing and safety compliance. Inspection, testing and feedback mechanisms are used to compare actual performance with specifications and initiate corrective measures. The principal objectives are to ensure conformity with standards, detect problems in raw materials and finished goods, minimize waste and rework, reduce the cost of poor quality and satisfy customers.

Quality Control addresses the standards and management practices required to ensure reliable and effective biofertilizer products. It introduces general quality concepts, quality-control parameters, microbial functions, and properties of microbial products. The chapter then explains quality-management procedures for important biofertilizer groups, including Rhizobium, nonsymbiotic nitrogen fixers, arbuscular mycorrhizal fungi, and phosphate-solubilizing bacteria. Quality-control measures are linked to product performance, consistency, storage, and application suitability. By emphasizing systematic assessment and management, the chapter highlights why quality assurance is essential for maintaining viable microbial populations and delivering predictable agricultural benefits from biofertilizers and related biological inputs. across production and distribution systems effectively today.

In this chapter, the importance of seafood in terms of their nutrition, and different methods of processing are dealt. Seafood quality and safety terminologies are explained briefly. The quality parameters affected by rigor mortis in raw, iced and cooked fish are given. Phenomenon of gaping described with reference to rigor mortis. Quality dimension of seafood is explained with intrinsic and extrinsic parameters. Various intrinsic factors affecting seafood quality such as f ish species, seasonal changes, parasite infestation and capture conditions are discussed in detail. The next part elucidates the sensory parameters affecting the seafood quality describing the objective and subjective method of quality assessments. Objective method covers both descriptive and discriminative tests. The final part mentions the Codex guidelines available for the conduction of seafood sensory assessment. At the end, the reader gains the knowledge on the basic information about seafood, role of rigor mortis in affecting seafood quality and the sensory quality assessment methods.

Objective

To learn the nutritional importance of seafood and their processing; rigor mortis and their role in seafood quality, intrinsic and extrinsic factors affecting seafood quality and sensory quality assessment methods.

1.1. Importance of Seafood

Seafood has been traditionally a part of diet in many parts of the world. In some countries like India, it constitutes the main supply of animal protein among coastal population. Seafood is a low-calorie food and easily digestible compared to other protein-rich meat and poultry products and hence, it is a good choice for elderly persons to obtain their daily protein requirements. Seafood has high-quality quality enriched with all the essential amino acids required for human health. It provides about 1/3rd of the average recommended daily allowance (RDA) of protein.

1. INTRODUCTION

Quality, i.e. the degree of excellence or superiority, is a combination of attributes, properties, or characteristics that each commodity value in of human food. The relative importance of each quality component depends upon the commodity and its intended use (e.g. fresh or processed) and varies among producers, handlers, and consumers. Appearance of quality, firmness and shelf-life are important from the point of view of wholesale and retail marketers. Consumers judge quality of fresh fruits and vegetables on the basis of appearance and firmness, flavour (eating) quality of the product. Consumers are also concerned about the nutritional quality and safety of fresh fruit and vegetables.